JP5080293B2 - Automatic analyzer and temperature abnormality detection method for liquid in reaction vessel - Google Patents

Description

  The present invention relates to an automatic analyzer that reacts a specimen with a reagent and analyzes the components of the specimen, and a liquid temperature abnormality detection method in the reaction container that detects a liquid temperature abnormality in the reaction container of the automatic analyzer. .

  Conventionally, in an automatic analyzer that analyzes a sample component by reacting a sample with a reagent and optically measuring the result of this reaction, the temperature of the liquid in the reaction vessel is kept constant. It is common to provide a constant temperature bath for storing a constant temperature liquid at a predetermined temperature around the periphery (see, for example, Patent Document 1). In this technology, it is assumed that the temperature of the thermostatic liquid is approximately equal to the temperature of the liquid in the reaction vessel, and the temperature of the liquid in the reaction vessel is adjusted to a desired set temperature by controlling the temperature of the thermostatic liquid. doing.

JP 2000-258425 A

  By the way, the temperature of the liquid in the reaction container may change as a new liquid is dispensed, and is not always equal to the temperature of the constant temperature liquid. However, when applying the above-described prior art, since it is assumed that the temperature of the constant temperature liquid and the temperature of the liquid in the reaction container are substantially equal, even if a temperature abnormality occurs in the liquid in the reaction container, As long as the temperature of the thermostatic liquid was normal, the temperature abnormality could not be detected.

  The present invention has been made in view of the above, and provides an automatic analyzer that can reliably detect an abnormal temperature of a liquid in a reaction vessel and a method for detecting an abnormal temperature of a liquid in a reaction vessel. Objective.

  In order to solve the above-described problems and achieve the object, an automatic analyzer according to the present invention reacts a sample and a reagent in a reaction container, and optically measures the result of the reaction, thereby measuring the sample. An automatic analyzer for analyzing a component, wherein a storage means for storing a calibration curve for giving the correlation of an absorbance-temperature correlation reagent having a correlation between absorbance and temperature, and when the absorbance-temperature correlation reagent is applied as a specimen and a reagent Conversion means for converting the absorbance of the absorbance-temperature correlation reagent obtained by optical measurement into temperature using the calibration curve stored in the storage means, and the temperature of the absorbance-temperature correlation reagent converted by the conversion means And determining means for determining the presence or absence of temperature abnormality of the absorbance-temperature correlation reagent in the reaction container according to a time change mode.

  Further, in the automatic analyzer according to the present invention, in the above invention, the determination unit is configured such that the temperature converted by the conversion unit is within a predetermined temperature range after the absorbance temperature correlation reagent is dispensed into the reaction container. On the basis of this time, the presence or absence of temperature abnormality of the absorbance temperature correlation reagent in the reaction container is determined.

  The automatic analyzer according to the present invention is the automatic analyzer according to the above invention, wherein the determination unit is configured so that a predetermined time elapses after the absorbance temperature correlation reagent is dispensed into the reaction vessel among the temperatures converted by the conversion unit. The presence or absence of temperature abnormality of the absorbance temperature correlation reagent in the reaction container is determined based on temperature.

  In the automatic analyzer according to the present invention, in the above invention, the temperature measuring means for measuring the temperature of the liquid contained in the reaction vessel, and the temperature measuring means when the absorbance temperature correlation reagent is applied as the liquid, And a calibration curve creating means for creating the calibration curve using the temperature to be measured and the absorbance of the absorbance temperature correlation reagent determined by the optical measurement.

  The automatic analyzer according to the present invention, in the above invention, comprises a reaction container holder that holds a plurality of the reaction containers, a heat retaining means that is provided in the vicinity of the reaction container holder, and that maintains a constant temperature, and the heat retaining apparatus. Temperature adjustment means for adjusting the temperature held by the means, wherein the calibration curve creating means measures the absorbance temperature correlation in the reaction container measured under a plurality of conditions in which the temperature held by the heat holding means is different from each other. The calibration curve is created using the absorbance and temperature of the reagent.

  Moreover, the automatic analyzer according to the present invention is characterized in that, in the above invention, the absorbance temperature correlation reagent is phenol red.

  The method for detecting a temperature abnormality of a liquid in a reaction container according to the present invention is an automatic analyzer that analyzes a component of the specimen by reacting a specimen and a reagent in the reaction container and optically measuring a result of the reaction. Is a method for detecting an abnormal temperature of a liquid in a reaction container, which detects an abnormal temperature of the liquid in the reaction container, and sequentially absorbs an absorbance-temperature correlation reagent having a correlation between absorbance and temperature into the reaction container as a sample and a reagent. On the other hand, an analytical operation step for obtaining the absorbance of the absorbance-temperature correlation reagent in the reaction container and a calibration curve that gives a correlation between the absorbance and the temperature in the absorbance-temperature correlation reagent are read from the storage means provided in the automatic analyzer. By using the read calibration curve, a conversion step for converting the absorbance obtained in the analysis operation step into a temperature, and conversion in the conversion step Characterized in that having, a determining step of determining whether the temperature abnormality of the absorbance temperature correlation reagents in the reaction vessel in accordance with aspects of the time variation of the temperature of the absorbance temperature correlation reagents.

  In the method for detecting a temperature abnormality of a liquid in a reaction container according to the present invention, in the above invention, the determination step is performed after the temperature converted in the conversion step dispenses the absorbance temperature correlation reagent into the reaction container. The presence or absence of temperature abnormality of the absorbance temperature correlation reagent in the reaction container is determined based on the time until entering a predetermined temperature range.

  In the method for detecting an abnormal temperature of a liquid in a reaction container according to the present invention, in the above invention, the determination step includes dispensing the absorbance-temperature correlation reagent among the temperatures converted in the conversion step into the reaction container. The presence or absence of temperature abnormality of the absorbance temperature correlation reagent in the reaction container is determined on the basis of the temperature after a predetermined time has elapsed.

  According to the present invention, an absorbance temperature correlation reagent having a correlation between absorbance and temperature is sequentially dispensed into a reaction container as a sample and a reagent, while an analysis operation for obtaining the absorbance of the absorbance temperature correlation reagent in the reaction container is performed to obtain an absorbance temperature. By using a calibration curve that correlates the absorbance and temperature in the correlation reagent, the absorbance obtained in the analysis operation is converted to temperature, and the converted absorbance in the reaction vessel is changed according to the mode of time change of the temperature of the temperature correlation reagent. Therefore, it is possible to accurately reflect the temperature in the reaction container. Therefore, the temperature abnormality of the liquid in the reaction container can be reliably detected.

  The best mode for carrying out the present invention (hereinafter referred to as “embodiment”) will be described below with reference to the accompanying drawings. Note that the drawings referred to in the following description are schematic, and when the same object is shown in different drawings, dimensions, scales, and the like may be different.

  FIG. 1 is a diagram showing a configuration of a main part of an automatic analyzer according to an embodiment of the present invention. The automatic analyzer 1 shown in FIG. 1 dispenses a specimen (sample) and a reagent into a reaction container, and optically measures a reaction occurring in the reaction container, and an automatic analysis including the measurement unit 101. And a data processing unit 201 that controls the apparatus 1 and analyzes the measurement result in the measurement unit 101, and the two units cooperate to automatically and biochemically analyze the components of a plurality of specimens. It is a continuous device.

  The measurement unit 101 includes a specimen container holder 4 that houses a plurality of racks 3 on which specimen containers 2 that contain specimens are mounted, a reagent container holder 6 that holds the reagent containers 5 and keeps them at a constant temperature, and specimens and reagents. A reaction container holder 8 that holds a reaction container 7 that reacts with each other, and a sample dispensing unit 9 that dispenses a sample contained in the sample container 2 held in the sample container holder 4 into the reaction container 7 by a metallic probe; A reagent dispensing unit 10 that dispenses the reagent contained in the reagent container 5 held in the reagent container holder 6 into the reaction container 7 by a metallic probe, a stirring unit 11 that stirs the liquid in the reaction container 7, A photometric unit 12 that receives analysis light irradiated from a light source and passes through the reaction vessel 7 and measures the intensity of a predetermined wavelength component, a temperature measurement unit 13 that measures the temperature of the liquid in the reaction vessel 7, and a reaction Wash the container 7 Cormorant includes a cleaning unit 14, a.

  FIG. 2 is a diagram showing the configuration of the reaction vessel holder 8 and its surroundings in the measurement unit 101. The reaction vessel holder 8 has a disk-shaped surface, a rotary table 81 that holds a plurality of reaction vessels 7 along the circumference of the surface, a shaft portion 82 that rotatably supports the rotary table 81, A drive unit 83 that rotates the shaft unit 82. The turntable 81 is formed using a material such as a metal having good thermal conductivity, and has a plurality of holding portions 811 that hold the reaction vessel 7. The holding unit 811 is provided with two windows 812 along the radial direction of the surface of the rotary table 81 for transmitting the analysis light projected from the photometry unit 12 toward the reaction vessel 7. The drive unit 83 is controlled by the rotation control unit 15.

  Below the reaction vessel holder 8, an annular thermostat 16 that circulates along the circumference of the rotary table 81 is provided. The thermostat 16 is made of a material having good thermal conductivity. A constant temperature liquid H is accommodated in a hollow portion that circulates and communicates in an annular shape inside the constant temperature bath 16. The constant temperature bath 16 has a concave portion 161 that surrounds the outer periphery of the holding portion 811 of the reaction vessel holder 8. For this reason, heat is transferred from the concave portion 161 to the reaction vessel 7 via the holding portion 811, and the temperature of the liquid inside the reaction vessel 7 approaches the temperature of the constant temperature liquid H. The thermostat 16 is provided with two windows (not shown) along the radial direction of the ring of the thermostat 16 for transmitting the analysis light projected by the light source of the photometry unit 12. The constant temperature bath 16 and the constant temperature liquid H are provided in the vicinity of the reaction vessel holder 8 and have at least a partial function of a heat retaining means that maintains a constant temperature.

  The temperature of the constant temperature liquid H is measured by the temperature sensor 17. The measurement result of the temperature sensor 17 is sent to the temperature control unit 18. The temperature control unit 18 controls driving of the heater 19 based on the measurement result of the temperature sensor 17. The heater 19 heats the constant temperature bath 16 under the control of the temperature control unit 18 and adjusts the temperature of the constant temperature liquid H. The set temperature of the constant temperature liquid H when the automatic analyzer 1 analyzes a normal sample is about the human body temperature (about 37 ° C.). The temperature control unit 18 and the heater 19 have at least a part of the function of the temperature adjusting unit that adjusts the temperature held by the heat retaining unit.

  FIG. 3 is a diagram illustrating a configuration of a main part of the temperature measurement unit 13. The temperature measurement unit 13 includes a thermistor 131 that measures the temperature inside the reaction vessel 7, an arm 132 that supports the thermistor 131 at the tip, a support shaft 133 that supports the base end of the arm 132 and can move up and down. The drive unit 134 moves the support shaft 133 up and down. The drive unit 134 is controlled by the drive control unit 20. The thermistor 131 moves up and down as the support shaft 133 moves up and down, and can move forward and backward with respect to the inside of the reaction vessel 7 (liquid storage portion) located at a position immediately below.

  The data processing unit 201 includes a keyboard, a mouse, and the like. The data processing unit 201 includes an input unit 21 for inputting information necessary for sample analysis and operation information of the automatic analyzer 1, a display and a printer, and information on sample analysis. And the like, and the absorbance of the liquid in the reaction container 7 is calculated based on the measurement result in the measurement unit 101, and the reaction is performed using the absorbance calculation result and various information such as a calibration curve and analysis parameters. A data generation unit 23 that calculates the components of the liquid inside the container 7, a calibration curve creation unit 24 that creates a calibration curve of an absorbance temperature correlation reagent having a correlation between absorbance and temperature, and a sample and a reagent that are absorbance temperature correlation reagents The conversion unit 25 that converts the absorbance measured during the analysis operation into a temperature using the calibration curve created by the calibration curve creation unit 24, and the temperature converted by the conversion unit 25 A determination unit 26 that determines the presence or absence of temperature abnormality of the absorbance temperature correlation reagent in the reaction container 7 according to the mode of change, a control unit 27 that controls the operation of the automatic analyzer 1, and a storage unit that stores various types of information 28. Among these, the control unit 27 is linked with the rotation control unit 15, the temperature control unit 18, and the drive control unit 20 of the measurement unit 101. In addition, the storage unit 28 stores the calibration curve created by the calibration curve creation unit 24, the determination conditions referred to by the determination unit 26, information about the sample and the reagent, and information including the analysis result of the sample.

  In the data processing unit 201 having the above-described configuration, the calibration curve creation unit 24, the conversion unit 25, the determination unit 26, and the storage unit 28 have at least some functions of the calibration curve creation unit, the conversion unit, the determination unit, and the storage unit. Each has. The data processing unit 201 is realized by a computer having a CPU, a ROM, a RAM, and the like.

FIG. 4 is a flowchart showing an outline of a calibration curve creation process performed by the automatic analyzer 1. In FIG. 4, the automatic analyzer 1 first adjusts the temperature of the constant temperature liquid H in the constant temperature bath 16 to a set temperature (step S1). The temperature of the constant temperature liquid H adjusted in step S1 has only a difference of about ± 0.5 ° C. from the temperature T ₀ set in the normal analysis. The temperature T ₀ is about the human body temperature (for example, about 37 ° C.).

  After the temperature of the constant temperature liquid H reaches the set value, the reagent dispensing unit 10 dispenses the absorbance temperature correlation reagent into the reaction container 7 (step S2). The absorbance-temperature correlation reagent has a correlation between absorbance and temperature. For example, phenol red can be applied. Hereinafter, the case where phenol red is applied as an absorbance temperature correlation reagent will be described.

  The reaction container 7 into which phenol red which is an absorbance temperature correlation reagent is dispensed is transferred to a position directly below the temperature measurement unit 13 by the rotation of the reaction container holder 8. The temperature measuring unit 13 measures the temperature of phenol red in the reaction container 7 by moving the thermistor 131 to the inside of the reaction container 7 after the support shaft 133 is lowered after the reaction container holder 8 is stationary (step) S3). The time from dispensing phenol red into the reaction vessel 7 in step S2 until measuring the temperature in step S3 is such that the temperature of phenol red in the reaction vessel 7 is substantially equal to the temperature of the constant temperature liquid H. The required time is set in advance.

  Thereafter, the automatic analyzer 1 performs photometry and absorbance calculation of the absorbance temperature correlation reagent (step S4). Specifically, after step S <b> 3, the reaction vessel holder 8 transfers the reaction vessel 7 whose temperature of phenol red is measured by the temperature measurement unit 13 and passes the photometry unit 12. The photometry unit 12 measures the intensity of the light that has passed through the reaction vessel 7 and sends the measurement result to the data generation unit 23. The data generation unit 23 calculates the absorbance of phenol red by using the measurement result of the photometry unit 12, and stores the calculated absorbance in association with the temperature measured by the temperature measurement unit 13 in the storage unit 28.

  The automatic analyzer 1 sets a plurality of temperatures of the constant temperature liquid H in advance, and repeats the processing of steps S1 to S4 for each set temperature to obtain a plurality of sets of absorbance and temperature. For this reason, the automatic analyzer 1 returns to step S1, and repeats a process, if the measurement process in all the preset temperature with respect to the constant temperature liquid H is not complete | finished (step S5, No). On the other hand, when the measurement process for all the set temperatures for the constant temperature liquid H is completed (step S5, Yes), the calibration curve creation unit 24 creates a calibration curve using the measurement result (step S6).

FIG. 5 is a diagram illustrating an example of a calibration curve created by the calibration curve creation unit 24. In FIG. 5, the horizontal axis represents absorbance and the vertical axis represents temperature. The calibration curve L is obtained by performing the processes of steps S1 to S4 described above twice, and the black dots indicate actual measurement values. The calibration curve L is a straight line and has the property that the absorbance increases as the temperature decreases. Thus, it is known that phenol red shows linearity in the relationship between absorbance and temperature in the vicinity of the set temperature T ₀ of the constant temperature liquid H during normal analysis. For this reason, when phenol red is applied as the absorbance temperature correlation reagent, if at least two sets of absorbance and temperature measurement values are obtained, a straight line passing through two points corresponding to the obtained two sets becomes a calibration curve. Therefore, when phenol red is applied as the absorbance temperature correlation reagent, the calibration curve creation process in the calibration curve creation unit 24 is facilitated. Note that the calibration curve is not limited to a straight line, but may be a curved line. When the calibration curve is a curve, the number of set temperatures of the constant temperature liquid H is preferably 3 or more.

  Next, a method for detecting a temperature abnormality in the liquid in the reaction vessel using the calibration curve created by the automatic analyzer 1 will be described with reference to the flowchart shown in FIG.

  First, the automatic analyzer 1 performs an analysis operation similar to a normal analysis operation using phenol red as the sample and the first and second reagents (step S11). Specifically, after the reagent dispensing unit 10 dispenses phenol red as the first reagent, the sample dispensing unit 9 dispenses phenol red as the sample, and the reagent dispensing unit 10 uses phenol red as the second reagent. To dispense. The amount to be dispensed into the reaction container 7 is small in the order of the first reagent, the second reagent, and the specimen. In particular, the amount of phenol red dispensed as the specimen is a small amount compared to the amount of phenol red dispensed as the first reagent. Since the reagent container holder 6 keeps the reagent at a low temperature, the phenol red dispensed as the first and second reagents is at a lower temperature than the room temperature phenol red dispensed as the specimen.

  In the course of the analysis operation of the automatic analyzer 1 in step S11, the reaction vessel holder 8 repeats a predetermined rotation operation, and therefore passes through the position where the photometry unit 12 is provided at a constant cycle. Since the photometry unit 12 always projects the analysis light during the analysis operation, it measures all the intensity of the light transmitted through the reaction vessel 7 passing through the optical path of the analysis light. Therefore, the photometry unit 12 performs a plurality of measurements on each reaction vessel 7 until a series of analysis operations are completed. The data measured by the photometry unit 12 is sent to the data processing unit 201 and used for calculation of absorbance in the data generation unit 23. The absorbance calculated by the data generation unit 23 is stored in the storage unit 28.

  Subsequent to the analysis operation described above, the conversion unit 25 converts all absorbances of phenol red calculated by the data generation unit 23 into temperatures by using the calibration curve stored in the storage unit 28 (step S12). .

  FIG. 7 is a diagram showing the relationship between the temperature T and time t of phenol red, in which the conversion unit 25 converts the absorbance. A curve C shown in FIG. 6 is a graph in which the temperature of phenol red in the reaction vessel 7 after the phenol red as the first reagent is dispensed in the reaction vessel 7 is arranged in time series. The black point on the curve C is a point corresponding to the measurement result, and gives a temperature-converted value of the absorbance at the time when it passes through the photometry unit 12 (photometry point). In the following, for convenience of explanation, the expressions specimen, first reagent, and second reagent are used, and these are all phenol red.

When the reagent dispensing unit 10 dispenses the first reagent into the reaction container 7 at time t ₁ , the dispensed first reagent is warmed by the constant temperature bath 16 and approaches the set temperature T ₀ of the constant temperature liquid H. Thereafter, the sample dispensing unit 9 dispenses the sample into the reaction container 7 at time t _S (t _S > t ₁ ). Since the dispensing amount of the specimen at room temperature is a small amount compared to the dispensing amount of the first reagent, the temperature change in the reaction vessel 7 due to the dispensing of the specimen is small. Therefore, the phenol red in the reaction vessel 7 becomes substantially constant at a temperature in the vicinity of the set temperature T ₀ before and after dispensing of the specimen. Thereafter, when the reagent dispensing unit 10 dispenses the second reagent into the reaction container 7 at time t ₂ (t ₂ > t _S ), the temperature of the second reagent is lower than the set temperature T ₀ , and therefore temporarily Accordingly, the temperature of the liquid inside the reaction vessel 7 is lowered. Thereafter, the temperature of the phenol red in the reaction vessel 7 rises again by being warmed in the thermostat 16 and reaches almost the set temperature T ₀ , and then becomes substantially constant.

After step S12, the determination unit 26 refers to the curve C to determine whether or not there is an abnormal temperature of phenol red in the reaction vessel 7 (step S13). Specifically, the determination unit 26 determines that the temperature of phenol red in the reaction vessel 7 is normal when the temperature (converted value) T of phenol red satisfies the following three conditions.
(1) A time Δt ₁ required from dispensing the first reagent to satisfying T ₀ −ΔT ≦ T ≦ T ₀ + ΔT is equal to or less than a predetermined value Δt ₀₁ (Δt ₁ <Δt ₀₁ ).
(2) The time Δt ₂ required to satisfy T ₀ −ΔT ≦ T ≦ T ₀ + ΔT after dispensing the second reagent is equal to or less than a predetermined value Δt ₀₂ (Δt ₂ <Δt ₀₂ ).
(3) Among the time zones in which the curve C is generated, the time zone from when the first reagent is dispensed until the predetermined time Δt ₀₁ elapses and the predetermined time Δt ₀₂ after the second reagent is dispensed are Except for the time period until the time elapses, T ₀ −ΔT ≦ T ≦ T ₀ + ΔT.
In the conditions (1) to (3), it is assumed that the temperature immediately after dispensing the first reagent and the second reagent is lower than T ₀ −ΔT. The predetermined constant ΔT is desirably about 0.5 ° C. at most.

  As a result of the determination by the determination unit 26, if any one of the above conditions (1) to (3) is not satisfied, the determination unit 26 determines that the phenol red in the reaction vessel 7 has a temperature abnormality ( Step S14, Yes). In this case, the output unit 22 outputs information notifying that there is an abnormality in the temperature of phenol red in the reaction vessel 7 (step S15). The operator performs maintenance of the automatic analyzer 1 based on the information output from the output unit 22.

  On the other hand, if the determination unit 26 determines that the conditions (1) to (3) described above are satisfied, the determination unit 26 determines that the temperature of phenol red in the reaction vessel 7 is normal (step S14, No). In this case, the automatic analyzer 1 ends the series of temperature abnormality detection processing, but may be automatically shifted to an analysis operation for a general sample or the like.

  Note that the determination condition when the determination unit 26 performs the determination in step S13 can be any one of the conditions (1) to (3), or the conditions (1) to (3) Any two of them can be used.

  It is also possible for the automatic analyzer 1 to periodically perform the processes of steps S11 to S15 at a predetermined cycle.

  In view of the fact that the amount of sample dispensed in the analysis operation is very small compared to the amount of reagent dispensed, the sample may not be dispensed in step S11. Thereby, it is not necessary to set the sample container 2 containing phenol red in the sample container holder 4 which is more complicated to set than the reagent container holder 6, and the temperature abnormality detection process can be performed more easily.

  According to the embodiment of the present invention described above, an absorbance-temperature correlation reagent having a correlation between absorbance and temperature is sequentially dispensed into a reaction container as a sample and a reagent, while the absorbance of the absorbance-temperature correlation reagent in the reaction container is determined. Perform the required analysis operation, and use the calibration curve that gives the correlation between the absorbance and the temperature in the absorbance temperature correlation reagent. Since the presence or absence of temperature abnormality of the absorbance temperature correlation reagent in the reaction container is determined according to the mode, the temperature in the reaction container can be accurately reflected. Therefore, the temperature abnormality of the liquid in the reaction container can be reliably detected.

  In addition, according to the present embodiment, since the temporal transition of the temperature state of the liquid in the reaction vessel when performing a series of analysis operations is grasped, the temperature abnormality of the liquid is detected under a plurality of conditions. It is possible to detect the temperature abnormality of the liquid with higher accuracy.

  Further, according to the present embodiment, since the automatic analyzer automatically creates a calibration curve, it is not necessary to prepare a separate calibration curve creation device. In addition, the burden on the user when creating a calibration curve can be reduced.

  In addition, according to the present embodiment, a calibration curve can be easily created because phenol red, which has a linear relationship between absorbance and temperature in the vicinity of the set temperature at the time of analysis, is used as the absorbance temperature correlation reagent.

The best mode for carrying out the present invention has been described so far, but the present invention should not be limited only by the above-described embodiment. For example, it is possible to perform temperature abnormality detection processing by sequentially dispensing phenol red as a specimen and a reagent to a plurality of reaction containers 7. In this case, the determination condition in the determination unit 26 may be set as follows.
(4) T ₀ −ΔT ′ ≦ T ≦ T ₀ + ΔT ′ when the temperature T converted from the absorbance with respect to the phenol red in each reaction vessel 7 has elapsed for a predetermined time after dispensing the first reagent. Meet. Here, ΔT ′ is approximately the same value as ΔT described above.

  The automatic analyzer according to the present invention can also be applied to a case where the holding portion of the reaction container holder is directly immersed in a constant temperature liquid.

  Thus, the present invention can include various embodiments and the like not described herein, and various design changes and the like can be made without departing from the technical idea specified by the claims. It is possible to apply.

It is a figure which shows the structure of the principal part of the automatic analyzer which concerns on one embodiment of this invention. It is a figure which shows the structure of the reaction container holder and its periphery. It is a figure which shows the structure of the principal part of a temperature measurement part. It is a flowchart which shows the outline | summary of the calibration curve creation process which the automatic analyzer which concerns on one embodiment of this invention performs. It is a figure which shows the example of the calibration curve which the automatic analyzer which concerns on one embodiment of this invention created. It is a flowchart which shows the outline | summary of a process of the temperature abnormality detection method of the liquid in the reaction container which concerns on one embodiment of this invention. It is a figure which shows the time change of the temperature (converted value from light absorbency) of the phenol red in reaction container.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Automatic analyzer 2 Specimen container 3 Rack 4 Specimen container holder 5 Reagent container 6 Reagent container holder 7 Reaction container 8 Reaction container holder 9 Specimen dispensing part 10 Reagent dispensing part 11 Stirring part 12 Photometric part 13 Temperature measuring part 14 Washing part DESCRIPTION OF SYMBOLS 15 Rotation control part 16 Constant temperature bath 17 Temperature sensor 18 Temperature control part 19 Heater 20 Drive control part 21 Input part 22 Output part 23 Data generation part 24 Calibration curve creation part 25 Conversion part 26 Determination part 27 Control part 28 Storage part 81 Rotary table 82 Shaft portion 83, 134 Drive portion 101 Measuring unit 131 Thermistor 132 Arm 133 Spindle 161 Recessed portion 201 Data processing unit 811 Holding portion 812 Window portion

Claims (9)

An automatic analyzer that analyzes a component of the specimen by reacting a specimen and a reagent in a reaction container and optically measuring a result of the reaction,
Storage means for storing a calibration curve that gives the correlation of the absorbance-temperature correlation reagent having a correlation between absorbance and temperature;
A conversion means for converting the absorbance of the absorbance temperature correlation reagent obtained by optical measurement when the absorbance temperature correlation reagent is applied as a specimen and a reagent into a temperature using the calibration curve stored in the storage means;
Determination means for determining the presence / absence of temperature abnormality of the absorbance temperature correlation reagent in the reaction container according to the mode of time change of the temperature of the absorbance temperature correlation reagent converted by the conversion means;
An automatic analyzer characterized by comprising: The determination means includes
Based on the time from when the temperature converted by the conversion means dispenses the absorbance temperature correlation reagent into the reaction container until it enters a predetermined temperature range, the temperature abnormality of the absorbance temperature correlation reagent in the reaction container The automatic analyzer according to claim 1, wherein presence or absence is determined. The determination means includes
Presence or absence of temperature abnormality of the absorbance temperature correlation reagent in the reaction container based on the temperature after a predetermined time has elapsed since dispensing the absorbance temperature correlation reagent into the reaction container among the temperatures converted by the conversion means The automatic analyzer according to claim 1, wherein the automatic analyzer is determined. Temperature measuring means for measuring the temperature of the liquid contained in the reaction vessel;
Creating a calibration curve using the temperature measured by the temperature measurement means when the absorbance temperature correlation reagent is applied as the liquid and the absorbance of the absorbance temperature correlation reagent determined by the optical measurement. Means,
The automatic analyzer according to any one of claims 1 to 3, further comprising: A reaction vessel holder for holding a plurality of the reaction vessels;
A heat retaining means provided in the vicinity of the reaction vessel holder and maintaining a constant temperature;
Temperature adjusting means for adjusting the temperature held by the heat retaining means;
With
The calibration curve creation means creates the calibration curve by using the absorbance and temperature of the absorbance temperature correlation reagent in the reaction container measured under a plurality of conditions with different temperatures held by the heat retention means. 5. The automatic analyzer according to claim 4, wherein   The automatic analyzer according to any one of claims 1 to 5, wherein the absorbance-temperature correlation reagent is phenol red. A reaction vessel in which an automatic analyzer that reacts a sample and a reagent in a reaction vessel and analyzes the components of the sample by optically measuring the result of the reaction detects an abnormal temperature of the liquid in the reaction vessel A temperature abnormality detection method for liquid in
An analytical operation step of sequentially calculating an absorbance-temperature correlation reagent having a correlation between absorbance and temperature as a specimen and a reagent, while obtaining the absorbance of the absorbance-temperature correlation reagent in the reaction container;
A calibration curve that gives correlation between absorbance and temperature in the absorbance temperature correlation reagent is read from the storage means provided in the automatic analyzer, and the absorbance obtained in the analysis operation step is converted into temperature by using the read calibration curve. A conversion step to
A determination step of determining whether there is a temperature abnormality of the absorbance temperature correlation reagent in the reaction container according to a mode of time change of the temperature of the absorbance temperature correlation reagent converted in the conversion step;
A method for detecting an abnormal temperature of a liquid in a reaction vessel. The determination step includes
Based on the time from when the temperature converted in the conversion step is dispensed into the reaction container, the temperature abnormality of the absorbance temperature correlation reagent in the reaction container. The method for detecting an abnormal temperature of a liquid in a reaction container according to claim 7, wherein presence or absence is determined. The determination step includes
Presence or absence of temperature abnormality of the absorbance temperature correlation reagent in the reaction container based on the temperature after a predetermined time has elapsed since dispensing the absorbance temperature correlation reagent into the reaction container among the temperatures converted in the conversion step The method for detecting a temperature abnormality of a liquid in a reaction container according to claim 7 or 8, wherein

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