JP5044179B2 - Method for producing deodorized organic fertilizer, deodorized organic fertilizer, and method for cultivating sugar beet or flower using the same - Google Patents

Description

  The present invention relates to a method for producing a deodorized organic fertilizer, a deodorized organic fertilizer, and a method for cultivating sugar beet or florets using the same. More specifically, the present invention relates to a method for producing a deodorized organic fertilizer characterized by mixing a specific porous carrier and a specific microorganism mixture with animal manure.

  Traditionally, compost production methods using domestic animal manure produce nitrogen compounds such as ammonia and hydrogen sulfide during fermentation, which causes bad odors and requires reworking. The problem is that it takes time, and a large-scale facility is required for composting without causing bad odor, and the following inventions have been made to solve the problem.

  For example, a method of composting livestock manure without generating bad odor by performing facultative anaerobic fermentation for about 2 to 5 months without forced aeration or reversal, and performing aerobic fermentation for about 1 month with reversal finish is reported. (See Patent Document 1). This method requires large equipment and composting takes time.

  The manufacturing method of pulverizing organic waste, mixing microorganisms, anaerobically fermenting, and drying makes it possible to fertilize in a short time without generating bad odors, but composting equipment is still necessary ( Patent Document 2).

  In addition, a method of collecting and culturing indigenous microorganisms and using them for compost production has been developed. In this method, it is necessary to cultivate microorganisms collected from mountain forests with time and effort (see Patent Document 3). .

  Furthermore, a compost produced by adding anaerobic inoculum PTA-1773 to organic materials such as shrimp and / or crab residue and fermenting at 50-90 ° C. under aerobic conditions has been invented. Since mixed bacteria with bacteria and / or actinomycetes are used, there is a problem that it is difficult to obtain mixed bacteria used for production (see Patent Document 4).

  In addition, a method for producing compost that has been mixed with plant raw materials such as straw, straw and dead leaves, chicken manure and Bacillus subtilis, turned over, and fermented for about 4 months to 1 year has been reported. The problem is that the required period is long (see Patent Document 5).

  As described above, various improvements have been studied. However, there has been reported a method for fertilizing animal manure without causing a bad odor such as ammonia, without turning it over, and without using a special device in a short period of time. Absent.

JP 2002-274986 A Japanese Patent Laid-Open No. 08-183686 JP 2004-305186 A Japanese Patent Laid-Open No. 2003-219864 Japanese Patent Laid-Open No. 05-301791

  An object of this invention is to provide the manufacturing method of the fertilizer which does not generate malodors, such as ammonia, and can be manufactured in a short period of time and low cost.

  The present inventors have found that by mixing a specific porous carrier and a specific mixed microorganism with animal manure, a fertilizer can be produced in a short period of time without generating malodor. Moreover, it discovered that this fertilizer accelerated | stimulated the growth of a plant and was very useful.

  That is, the present invention relates to Bacillus subtilis, Bacillus rikenformis, Bacillus circulans, Bacillus polymixer, Clostridium cellulolyticum, Clostridium aerotolerance, Bacillus azotofixance, Bacillus macerans, Clostridium acetobutylicum and The present invention relates to a method for producing a deodorized organic fertilizer characterized by mixing and fermenting a mixed microorganism comprising Clostridium pasterianum, a porous carrier selected from Shirasu and / or Shirasu balloon, and animal manure.

  In the said manufacturing method, it is preferable to manufacture without performing turnover during fermentation.

  In the production method, the fermentation period is preferably 20 to 50 days.

  The present invention also relates to a deodorized organic fertilizer obtained by the production method.

  The present invention also includes Bacillus subtilis, Bacillus rikenformis, Bacillus circulans, Bacillus polymixer, Clostridium cellulolyticum, Clostridium aerotolerance, Bacillus azotofixance, Bacillus macerans, Clostridium acetobutylicum and Clostridium. -The present invention relates to a deodorized organic fertilizer formed by mixing a mixed microorganism comprising Pasterianum, a porous carrier selected from Shirasu and / or Shirasu balloon, and animal manure.

  The present invention further relates to a method for cultivating sugar beet or florets, characterized in that the fertilizer is used.

  In the production method of the present invention, an excellent organic fertilizer can be produced in a short period of time and labor saving without generating malodors, and no specially large equipment is required.

  In the production method of the present invention, the excrement of domestic animals such as cattle, chickens and pigs is suppressed, and the generation of malodorous substances such as ammonia that are emitted when the fungi in these excretions ripen the fiber, etc. It can be used as a deodorized organic fertilizer without spending time, labor, and expenses without performing work such as turning over. For this reason, the deodorized organic fertilizer obtained is useful because it contains more elements such as nitrogen, phosphorus and potassium contained in the excrement than normal compost.

  The fertilizer of the present invention can grow sugar beet or florets in a short period of time without generating malodor.

  Further, since the fertilizer of the present invention has a high ability to grow crops, it is possible to easily cultivate sugar beet or flowers in a small amount of mixed soil, a small box and a narrow space, and it is very suitable for upland cultivation. .

  Furthermore, since the natural strength of the field is restored by the use of the fertilizer of the present invention, the antibacterial and anti-pest properties of sugar beet or flowers are increased, and it is possible to cultivate sugar beet or flowers without depending on agricultural chemicals or chemical fertilizers. Become.

  The mixed microorganisms used in the present invention include Bacillus subtilis, Bacillus rikenformis, Bacillus circulans, Bacillus polymixer, Clostridium cellulolyticum, Clostridium aerotolerance, Bacillus azotofix, Bacillus macerans, Clostridium. Acetobutylicum and Clostridium pasterianum, both of which are derived from soil. These microorganisms can reproduce in anaerobic conditions, usually in the presence of plant fibers in the soil. These are bacteria that are generally used for testing and research, and are commercially available or readily available that are deposited at microorganism depository institutions and microorganism preservation institutions. The mixed microorganism of the present invention preferably contains lipopeptide-producing bacteria, cellulase-producing bacteria, and nitrogen-fixing bacteria. Specifically, for example, Bacillus subtilis ATCC No. 21332 and 6051 and Bacillus rikenformis ATCC No. 39307 and 14580, Bacillus circulans ATCC No. 9500, Bacillus polymixer ATCC No. 842, Clostridium cellularity cam ATCC No. 35319 and Clostridium Aerotolerance ATCC No. 43524, Bacillus azotofixans ATCC No. 35681, Bacillus macerans ATCC No. 8244, Clostridium acetobutylicum ATCC No. 8 824 and Clostridium pasterianum ATCC No. It is preferred to use a strain such as 6013.

  The proportion of each microorganism in the mixed microorganism of the present invention is not particularly limited, but it is preferable to add an equal amount by dry cell weight.

  As the mixed microorganism of the present invention, a dry cell in a quiescent state in which intracellular moisture is dried and the activity of the microorganism is stopped except for a liquid phase which is a place of metabolic function of the cell may be used as it is, or dry You may use the microbial cell in the form suspended in the culture solution. Moreover, it can also be used as a mixed microorganism preparation produced by cultivating dried bacterial cells in a culture solution and then mixing and immobilizing the bacterial cell suspension with a small amount of a porous carrier. From the viewpoint of easy handling and easy mixing with a large amount of manure and porous carrier, it is preferable to use a mixed microorganism preparation.

  The mixed microorganism preparation can be produced by mixing a microorganism suspension obtained by suspending microorganisms in a culture solution with a porous carrier. Specifically, 500 mg of a culture solution is prepared by mixing molasses in water at a ratio of 1 mg to 3 mg, and 0.3 g to 0.5 g of dry mixed microorganisms in which each microorganism is mixed in an equal amount by dry weight is added. This can be produced by culturing for 2 to 3 days, mixing with 10 kg to 20 kg of shirasu (water content 0% to 5%) having a particle size of 20 μm to 3 mm, and immobilizing.

  The porous carrier used in the present invention is selected from Shirasu and / or Shirasu balloon.

  Shirasu is generally grayish white, semi-consolidated, porous and composed of a wide range of particle shapes, most of which consists of sand and granular sand. Mineral composition is mainly composed of volcanic glass and plagioclase, and pyroxene, quartz, magnetic steel, etc. are minor components. The true specific gravity of Shirasu is in the range of 2.3 to 2.5. The chemical composition contains about 70% by mass of silicic acid, about 14% by mass of alumina, and about 8% by mass of alkali oxide. Shirasu can be used as it is, but may be used after heat sterilization.

  The shirasu balloon is a porous and spherical product produced by heat-treating shirasu at 800 to 1200 ° C. and foaming. It is a lightweight material because of its low specific gravity, and is an environmentally friendly material that is colorless and harmless and has excellent chemical resistance.

  The particle diameters of the shirasu and shirasu balloon used in the present invention are preferably 20 μm to 3 mm. At 20 μm or less, water retention is reduced, and since there are few pores that can be used to absorb microorganisms, it is not very suitable for fixing as a microbial preparation. Fertilizer manufactured with a particle size of 3 mm or more is mixed with soil in fields, rice fields, or pots. Sometimes the particle size is too large, and the aggregate structure is broken, which is not preferable.

  Furthermore, the animal manure used in the present invention is not particularly limited, but any one or two or more of cow manure, chicken manure, and pig manure can be selected and used.

  The three elements necessary for crops, nitrogen, phosphorus and potassium, are 2.5% for raw cow dung, 4.1% phosphorus, 0.3% potassium, and 5.0% nitrogen for raw chicken dung. , Phosphorus 4.7%, Potassium 2.3%, Pig manure raw nitrogen 4.3%, Phosphorus 4.9%, Potassium 0.6% (Fertilizer manual, 4th edition, corporation, farming and fishing village culture However, since conventional compost takes too much time to produce, the content of these three elements is very low. However, in the present invention, manure can be used in a short period of time, so that a very excellent fertilizer having a three-element content higher than that of normal compost can be obtained.

  Examples of raw materials that can be added to the fertilizer of the present invention include waste cultivated enoki mushrooms and charcoal. Further, rice bran, agricultural waste, domestic waste, rice husk, sugarcane bacus, rice flour, etc., which are usually used for producing fertilizer and compost may be added. Of these, rice flour and the like are rich in amino acids and the like, and it is preferable to add 1 to 3 kg to 10 kg of feces depending on the purpose.

  The method for producing a deodorized organic fertilizer of the present invention includes, for example, mixing a mixed carrier, animal excrement, and a porous carrier selected from Shirasu and / or Shirasu balloon, sealing the surface, and leaving it for several tens of days. Can be done.

  Another method for producing the deodorized organic fertilizer of the present invention includes, for example, a mixed microorganism preparation in which a porous carrier selected from Shirasu and / or Shirasu balloon is impregnated with a culture solution of a mixed microorganism and fixed to animal manure and It can be performed by mixing with a porous carrier, sealing the surface, and leaving it for several tens of days.

  The fertilizer of the present invention can be produced at any place, for example, on concrete and soil. The surface may have any shape as long as it can be sealed, and it is convenient and preferable to cover the surface with a vinyl sheet.

  Usually, the generation of ammonia causes a germination disorder and an initial development disorder, so it takes several months to half a year before animal manure can be used as compost. Since there is almost no generation | occurrence | production, it can be set as the usable fertilizer which does not hurt a root | route even after sowing and planting in 20 to 50 days after mixing of mixed microorganisms. If the fermentation period is shorter than 20 days, ammonia may remain to some extent, which may inhibit plant growth. When it is longer than 50 days, nutrients such as nitrogen, phosphorus and potassium are decreased, and the efficacy as a fertilizer tends to be decreased.

  The mixing ratio of each component is preferably 2 to 3% for mixed microorganisms and 5% to 7% for the porous carrier with respect to the weight of animal manure.

  The fertilizer of the present invention can be used without particular limitation for the cultivation of various sugar beet or florets. Suitable examples include mizuna, komatsuna, mini radish, mini carrot, mini turnip, onion, salmon, green peas, spinach, cucumber, bitter gourd, eggplant, red cabbage, broad bean, sweet pea, chrysanthemum and chrysanthemum.

  Hereinafter, the present invention will be described in detail with reference to examples, but the present invention is not limited thereto.

Production Example 1
The microorganisms listed in Table 1 were mixed in equal amounts by dry weight to prepare dry mixed microorganisms. 0.3 g of this dry mixed microorganism was added to a culture solution containing 2 g of molasses in 500 mg of water, and cultured at 18-23 ° C. for 4 days. This was mixed and fixed in 10 kg of shirasu (water content 0% to 5%) having a particle size of 20 μm to 3 mm to produce a mixed microorganism preparation A.

Production Example 2
A mixed microorganism preparation B was produced in the same manner as in Production Example 1 except that 5 kg of Shirasu Balloon WB-601 (manufactured by Axes Chemical Co., Ltd.) having an average particle diameter of 141.92 μm was used instead of Shirasu.

  In the following Examples 1-6 and Comparative Examples 1-6, all fertilizers were manufactured by sealing the surface with a vinyl sheet and fermenting without turning back.

Example 1 and Comparative Example 1
10 kg of cow dung, 10 kg of enoki waste culture soil, 200 g of mixed microbial preparation A and 10 kg of shirasu collected in Kokubun Kiyomizu Forest of Kirishima City, Kagoshima Prefecture, or 10 kg of cow dung waste and 10 kg of enoki mushroom soil. (Comparative Example 1) was mixed and placed on the soil and fermented for 30 days from July 10 to August 8, 2002.

  In Example 1, the compost did not become sludge, and the generation of malodor could be suppressed, but the fertilizer of Comparative Example 1 became sludge in the vinyl sheet, and the eyes were opened with malodor and gas. I was unable to do so. Changes in temperature and ammonia concentration during fermentation were measured on the first, tenth, twenty and thirty days using a GASTEC detector tube type gas meter (scale range: 1 to 30 ppm; manufactured by GASTECH, product number GV-100S) did. The results are shown in Table 2.

  From Table 2, the fertilizer of Example 1 had a mild temperature change, and the fermentation proceeded gradually. Moreover, it became clear that the ammonia concentration dropped to about one third on the 10th day, and almost no ammonia was generated on the 20th day. In the fertilizer of Comparative Example 1, a rapid temperature increase continued until the 30th day. As for the ammonia concentration, the upper limit of detection was already 30 ppm or more on the 10th day, and a high ammonia concentration of 30 ppm or more was shown until the 30th day.

Example 2 and Comparative Example 2
In farmland in Kirishima City, Kagoshima Prefecture, 1t of cow dung, mixed microorganism preparation A1kg and Shirasu 10kg (Example 2) collected from Kiribun Shimizu Forest in Kirishima City, Kagoshima Prefecture are mixed and stacked in the field, or only 1t of cow dung (comparative example) 2) was placed on the soil and fermented from October 3rd to November 3rd, 2003 for 31 days.

  In Example 2, the compost did not become sludge, and it was possible to suppress the generation of malodor, but the fertilizer of Comparative Example 2 became sludge in the vinyl sheet, and the eyes were opened with malodor and gas. I was unable to do so. Changes in temperature and ammonia concentration during fermentation were measured on the first, tenth, twenty and thirty days using a GASTEC detector tube type gas meter (scale range: 1 to 30 ppm; manufactured by GASTECH, product number GV-100S) did. The results are shown in Table 3.

  From Table 3, the fertilizer of Example 2 had a mild temperature change, and fermentation proceeded gradually. Moreover, it became clear that the ammonia concentration dropped to about a half on the 10th day, and almost no ammonia was generated on the 20th day. In the fertilizer of Comparative Example 2, the rapid temperature increase continued until the 30th day. As for the ammonia concentration, the upper limit of detection was already 30 ppm or more on the 10th day, and a high ammonia concentration of 30 ppm or more was shown until the 30th day.

  Moreover, in Example 2, weed growth was observed around the accumulated fertilizer, and the weed grew after about 20 days in the vinyl sheet. Thus, in Example 2, it was demonstrated that as fermenting progresses, no fertilizer generates gas that would harm plants such as ammonia gas.

Example 3 and Comparative Example 3
In farmland in Kirishima City, Kagoshima Prefecture, 1 t of cow dung, 1 kg of mixed microorganism preparation, 5 kg of charcoal, 10 kg of rice bran, and 10 kg of shirasu collected in the mountain forest of Kokubun Kiyomizu, Kirishima City, Kagoshima Prefecture (Example 3) or 1 kg of cow dung and 10 kg of rice bran (comparative example) 3) was mixed and piled up on the soil of the field, and fermented for 32 days from January 13 to February 13, 2004.

  In Example 1, the compost did not become sludge, and the generation of malodor could be suppressed, but the fertilizer of Comparative Example 1 became sludge in the vinyl sheet, and the eyes were opened with malodor and gas. I was unable to do so. Changes in temperature and ammonia concentration during fermentation were measured on the first, tenth, twenty and thirty days using a GASTEC detector tube type gas meter (scale range: 1 to 30 ppm; manufactured by GASTECH, product number GV-100S) did. The results are shown in Table 4.

  From Table 4, the temperature change of the fertilizer of Example 3 was gentle, and fermentation proceeded gradually. Moreover, it became clear that the ammonia concentration dropped to about three-quarters on the 10th day, and almost no ammonia was generated on the 30th day. In the fertilizer of Comparative Example 3, the temperature increased rapidly until the 20th day. As for the ammonia concentration, the upper limit of detection was already 30 ppm or more on the 10th day, and a high ammonia concentration of 30 ppm or more was shown until the 30th day.

  Moreover, in Example 3, weed growth was recognized around the piled fertilizer, and weeds grew after about 20 days in the vinyl sheet. Thus, in Example 3, it was demonstrated that as fermenting progresses, no gas that would harm plants such as ammonia gas was generated from the fertilizer.

Example 4 and Comparative Example 4
(Example 4) Mixing and stacking 10t of cattle manure, 10kg of mixed microorganism preparation A, 20kg of charcoal, 40kg of rice bran, and 40kg of shirasu collected from the forests of Kiiri-cho, Kagoshima Alternatively, 2 t of cow dung was stacked (Comparative Example 4) and fermented for 30 days from February 5, 2004 to March 5, 2004.
In Example 4, it was possible to suppress the generation of malodor and gas, but the fertilizer of Comparative Example 4 generated malodor and gas. Changes in temperature and ammonia concentration during fermentation were measured on day 1, 10, 20 and 29 using a GASTEC detector tube type gas meter (scale range: 1 to 30 ppm; manufactured by GASTECH, product number GV-100S) did. The results are shown in Table 5.

  From Table 5, the temperature change of the fertilizer of Example 4 was gentle, and fermentation proceeded gradually. Moreover, it became clear that the ammonia concentration dropped to about a half on the 10th day, and almost no ammonia was generated on the 20th day. In the fertilizer of Comparative Example 3, the temperature increased rapidly until the 20th day. As for the ammonia concentration, the upper limit of detection was already 30 ppm or more on the 10th day, and a high ammonia concentration of 30 ppm or more was shown until the 29th day. As a result, it was demonstrated in Example 4 that as fermenting progresses, no fertilizer generates gas that would harm plants such as ammonia gas.

Example 5 and Comparative Example 5
Cattle manure 10t, mixed microorganism preparation B10kg, charcoal 20kg, rice bran 40kg and Shirasu Balloon SSB-400 (manufactured by Axes Chemical Co., Ltd., 49.3-1.4 microns) (Example 5) or cow dung 2t was stacked (Comparative Example 5) and fermented for 32 days from May 20 to June 20, 2004.

  In Example 5, it was possible to suppress the generation of malodor and gas, but the fertilizer of Comparative Example 5 generated malodor and gas. Changes in temperature and ammonia concentration during fermentation were measured on the first, tenth, twenty and thirty days using a GASTEC detector tube type gas meter (scale range: 1 to 30 ppm; manufactured by GASTECH, product number GV-100S) did. The results are shown in Table 6.

  From Table 6, the temperature change of the fertilizer of Example 5 was gentle, and fermentation proceeded gradually. Moreover, it became clear that the ammonia concentration dropped to about a half on the 10th day, and almost no ammonia was generated on the 20th day. In the fertilizer of Comparative Example 5, the rapid temperature increase continued until the 30th day. As for the ammonia concentration, the upper limit of detection was already 30 ppm or more on the 10th day, and a high ammonia concentration of 30 ppm or more was shown until the 30th day. As a result, in Example 5, it was demonstrated that as fermenting progresses, no gas that would harm plants such as ammonia gas was generated from the fertilizer.

Example 6 and Comparative Example 6
On the concrete in the parking lot in Osaki-cho, Kagoshima Prefecture, 500 kg of cow dung, 500 kg of chicken dung, 1 kg of mixed microorganism preparation, and 10 kg of shirasu collected from the mountain forest of Osaki-cho, Kagoshima Prefecture (Example 6), or 500 kg of cow dung and chicken dung 500 kg (Comparative Example 6) was mixed and stacked, and fermented for 32 days from August 21, 2005 to September 21, 2005.

  In Example 6, the generation of malodor and gas could be suppressed, but the fertilizer of Comparative Example 6 became sludge in the vinyl sheet and generated malodor and gas. Changes in temperature and ammonia concentration during fermentation were measured on the first, tenth, twenty and thirty days using a GASTEC detector tube type gas meter (scale range: 1 to 30 ppm; manufactured by GASTECH, product number GV-100S) did. The results are shown in Table 7.

  From Table 7, the fertilizer of Example 6 had a mild temperature change, and the fermentation proceeded gradually. Moreover, it became clear that the ammonia concentration dropped to about one third on the 20th day, and almost no ammonia was generated on the 30th day. In the fertilizer of Comparative Example 6, the rapid temperature increase continued until the 20th day. As for the ammonia concentration, the upper limit of detection was already 30 ppm or more on the 10th day, and a high ammonia concentration of 30 ppm or more was shown until the 30th day. Thus, in Example 6, it was demonstrated that as fermenting progressed, no fertilizer generated gas that would harm plants such as ammonia gas.

Example 7 Mizuna Cultivation Mix 5kg of fertilizer of Example 1, 2.5kg of black soil and 2.5kg of humus soil, put them in a plastic box for raising seedlings (W60cm x D30cm x H4cm) in a net house, and on September 5 Several 20 mizuna seeds were planted at 5 cm intervals. It germinated after 4 days, and then thinned out to one strain at one place, and all 20 strains could be harvested after 40 to 45 days. Leaf length grew to 25-30 cm.

  Also, 1 kg of fertilizer from Kunsei Kasei No. 7 (Mitsubishi Chemical Co., Ltd.), 3 kg of black soil and 3 kg of humus were mixed, and on April 5, 20 seeds of mizuna were planted at 5 cm intervals. Germination occurred after 4 days, and 17 strains could be harvested from 45 to 65 days later, but 3 strains were not large enough to be harvested. The length of the harvested leaves was 15-20 cm.

Example 8 Komatsuna cultivation 5 kg of fertilizer of Example 1, 2.5 kg of black soil and 2.5 kg of humus soil were mixed, and on September 5 in a plastic box for raising seedlings in a net house (W 60 cm x D 30 cm x H 4 cm) Into this, several seeds of Komatsuna were seeded at 40 places at intervals of 5 cm. It germinated after 4 days, thinned out after 12 days to give one strain at one place, and all 40 strains could be harvested after 44 to 49 days. Leaf length grew to 26-33 cm.

  We mixed 1 kg of fertilizer from Kunsei Kasei 7 (Mitsubishi Chemical Co., Ltd.), 3 kg of black soil and 3 kg of humus soil, and on April 5, we planted several seeds of komatsuna at several intervals of 5 cm. It germinated after 4 days, and it was thinned out on the 15th day to make one strain at one place, and 15 strains could be harvested from 50 to 60 days, but 25 strains were not large enough to be harvested. The length of the harvested leaves was 15-20 cm.

Example 9 Mini Radish Cultivation 5 kg of fertilizer of Example 1, 2.5 kg of black soil, and 2.5 kg of humus soil are mixed and placed in a plastic box (W45 cm x D35 cm x H8 cm) in a net house. Several radish seeds were planted at intervals of 5 cm at 40 locations. It germinated after 5 days, thinned out after 9 days to give one strain at one place, and all 40 strains could be harvested after 49 days. The mini radish had a diameter of 2 to 3 cm and a length of 5 to 6 cm.

  We mixed 1 kg of fertilizer and 5 kg of black soil and 3 kg of humus soil from Kunsei Kasei No. 3 (manufactured by Katakura Chikkarin Co., Ltd.). On February 28, 40 seeds of mini radish were seeded at 4 cm intervals. It germinated after 5 days, and after 10 days, it was thinned to one strain at one place, and 38 strains could be harvested from 45 to 60 days later, but the two strains were not large enough to be harvested. The harvested mini radish had an average diameter of about 2.0 cm and a length of about 4.9 cm.

Example 10 Mini Turnip Cultivation Mix 5kg of fertilizer of Example 1, 2.5kg of black soil, and 2.5kg of humus soil and put it in a plastic box (W45cm x D35cm x H8cm) on February 28th. Several 40 pieces were placed at 5 cm intervals. It germinated after 5 days, thinned out after 9 days to give one strain at one place, and all 40 strains could be harvested after 49 days. The mini-cube grew to 2 to 3 cm in diameter and 2 to 3 cm in length.

  We mixed 1 kg of fertilizer and 5 kg of black soil and 3 kg of humus soil from Kunsei Kasei No. 3 (manufactured by Katakura Chikkarin Co., Ltd.). It germinated after 5 days and thinned out after 10 days to give one strain at one place, and 35 strains could be harvested from 45 to 60 days later, but 5 strains were not large enough to be harvested. The harvested mini-cubs grew to an average diameter of about 1.9 cm and an average length of about 2 cm.

Example 11 Chrysanthemum cultivation 5 kg of fertilizer of Example 1, 2.5 kg of black soil, and 2.5 kg of humus soil were mixed and placed in a planter (W40 cm × D20 cm × H25 cm). Transplanted on the day. A month later, Chrysanthemam grew to a height of about 30 cm and became so large that it overflowed from the planter.

  1 kg of fertilizer, 3 kg of black soil, and 3 kg of humus soil from Union Chemical No. 3 (manufactured by Katakura Chikkarin Co., Ltd.) were mixed, and two strains about 10 cm in height were transplanted on July 13. One month later, Chrysanthemam grew to a height of about 25-28 cm.

Example 12 Onion cultivation 250 kg of the fertilizer of Example 2 was sown in a 5 tsubo field, and the onion was transplanted on December 21 and harvested on April 5.

  In the field used this time, only the onion of about 10 cm in diameter was produced even if it applied union chemical No. 7 (Mitsubishi Chemical Co., Ltd.), normal cow dung, and chicken dung for the past 10 years, but the fertilizer of Example 2 was used. The onion of diameter 12-15cm was made by giving. No pesticides were used.

Example 13 Strawberry cultivation 500 kg of the fertilizer of Example 2 was planted in a 10 tsubo field, and strawberries were transplanted on December 21 and harvested on March 21. In this field, union chemical 7 (manufactured by Mitsubishi Chemical Co., Ltd.), ordinary cow dung, and chicken dung, only a strawberry with a diameter of about 2 cm was produced, but the fertilizer of Example 2 was applied. Many strawberries with a diameter of 3 to 4 cm, which is almost twice the average year, were made. In the usual year, the insecticide bactericide Olate solution (Otsuka Chemical Co., Ltd.) sprayed three or four times was sprayed only once.

Example 14 Green pea cultivation 250 kg of the fertilizer of Example 2 was sown in a 5 tsubo field, the green peas were sown on December 28, and harvested on March 28. In this field, IB Kasei (manufactured by Mitsubishi Chemical Aguri Co., Ltd.) and normal cow dung only grew to about 1.7m in height, but the fertilizer of Example 2 was applied. And grew to about 2.2m in height. The pesticide marathon emulsion (manufactured by Nippon Agricultural Chemicals Co., Ltd.) sprayed four or five times a year only needs to be sprayed only twice when the fertilizer of the present invention is applied.

Example 15 Spinach cultivation In a greenhouse in Aya-cho, Miyazaki Prefecture, a soil sampled from a mountain forest in Aya-machi is placed on a stand of 0.7 m height x 1.2 m width x 15 m length, and spinach is added. When 50 rows were seeded at intervals of 15 cm, germination occurred after 5 or 6 days. Twenty days later, 15 kg of the fertilizer of Example 3 was applied to 15 rows out of 50 rows, and 20 kg of the chemical fertilizer mixture was added to the remaining 35 rows, and phosphorus ammonium sulfide 555 (manufactured by Corp Chemical Co., Ltd.) was applied. Then, 60 days after germination, spinach fertilized with chemical fertilizer showed only 5-10 cm of growth and was not yet large enough to be harvested compared to spinach fertilized with fertilizer of Example 3. Grew to 25-30 cm and could be harvested.

Example 16 Cucumber cultivation 4 t of the fertilizer of Example 4 was added to 300 tsubo of farmland in Ibusuki City, Kagoshima Prefecture, and cucumbers were cultivated. On September 2, 20 cucumber strains of about 20 cm were transplanted and harvested on October 20. The length of the cucumbers was 30-40 cm and the diameter was 5-8 cm, growing 30% larger than that of the usual year, and the yield increased by about 20%. There was no generation of pests, and no pesticides were sprayed.

Example 17 Bitter gourd cultivation 4t of the fertilizer of Example 4 was introduced into 300 tsubo of farmland in Ibusuki City, Kagoshima Prefecture, and bitter gourd was grown. On September 5, 10 seedlings of bitter gourd approximately 20 cm were transplanted and harvested on November 20. The bitter gourd was 20-30 cm in length and 8-10 cm in diameter, growing about 35% larger than the average year, and the yield increased by 20%. Pesticides were not used during this period, but no pests were generated.

Example 18 Eggplant cultivation 4 t of the fertilizer of Example 4 was introduced into 300 tsubo of farmland in Ibusuki City, Kagoshima Prefecture. On September 5, 10 seedlings of approximately 15 cm of eggplant were transplanted and harvested on November 15. The length was 20-30 cm and the diameter was 5-8 cm, growing about 20% larger than the usual one, and the yield increased by 25%. Pesticides were not used during this period, but no pests were generated.

Example 19 Red Cabbage Cultivation 6 t of the fertilizer of Example 5 was introduced into 400 tsubo of farmland in Ibusuki City, Kagoshima Prefecture, and on October 15 a 10 cm red cabbage seedling was transplanted. The outer leaves of the normal year were 15-20 cm 66 days after transplanting, whereas the outer leaves of the red cabbage to which the fertilizer of the present invention was applied grew greatly to 30-45 cm. In addition, the pesticide spraying performed four times in the normal year was not performed once.

Example 20 Broad bean cultivation 12 t of the fertilizer of Example 5 was introduced on 1000 tsubo of farmland in Ibusuki City, Kagoshima Prefecture on August 25, and 2-5 cm broad bean seedlings were transplanted at 50 cm intervals on September 4. The first harvest was made on November 10, about one week earlier than the usual harvest schedule. The damage caused by root rot and aphids was reduced to 10% compared to 20% in the previous year. The spraying of the disinfectant Daisen Stainless (manufactured by Dow Chemical Japan Co., Ltd.) was performed twice, while it was performed four times a year. Adjuvant Affirm Emulsion (manufactured by Syngenta Japan Co., Ltd.) was used 4 times compared to 6 times per year.

Claims (6)

A mixture containing Bacillus subtilis, Bacillus rikenholmis, Bacillus circulans, Bacillus polymixer, Clostridium cellulolyticum, Clostridium aerotolerance, Bacillus azotofixance, Bacillus macerans, Clostridium acetobutylicum and Clostridium pasterianum A method for producing a deodorized organic fertilizer, characterized by mixing and fermenting a porous carrier selected from microorganisms, shirasu and / or shirasu balloon, and animal manure. The production method according to claim 1, wherein the production is performed without turning back during the fermentation. The production method according to claim 1 or 2, wherein the fermentation period is 20 to 50 days. The deodorized organic fertilizer obtained by the manufacturing method of Claim 1. A mixture consisting of Bacillus subtilis, Bacillus rikenholmis, Bacillus circulans, Bacillus polymixer, Clostridium cellulolyticum, Clostridium aerotolerance, Bacillus azotofixance, Bacillus macerans, Clostridium acetobutylicum and Clostridium pasterianum A deodorized organic fertilizer obtained by mixing a porous carrier selected from microorganisms, shirasu and / or shirasu balloon, and animal manure. A method for cultivating sugar beet or florets, wherein the fertilizer according to claim 4 or 5 is used.