JP5037098B2 - Agricultural and horticultural compositions - Google Patents

Description

  The present invention relates to a composition for agricultural and horticultural use containing a bacterium belonging to the genus Bacillus and a specific chemical pesticide, which can reduce the application amount of the chemical pesticide and reduce the burden on the environment.

  Control of crop diseases and insects, and weeds that adversely affect crop growth include crop rotation and field conversion, cultivated or physical control using solar heat, chemical control using chemical pesticides, and control by using pheromones against pests. Control by using pest resistant varieties, antagonistic microorganisms against attenuated viruses and pathogens, biological control using natural enemies against pests, mites, nematodes and the like. Among these, development research on chemical pesticides, especially organic synthetic pesticides, has developed remarkably until recently, and many drugs with high efficacy and various actions have been developed one after another, and various application methods have also been developed. It was. Chemical control methods using these are widely used because they greatly contribute to the control of pests and weeds in crops and further save labor in control work.

  However, these chemical pesticides sometimes affect the survival of organisms in the surrounding environment as well as the target to be controlled. In order to reduce the environmental impact of chemical pesticides as much as possible, for example, we devised spraying methods and amounts so that chemical pesticides do not flow into water systems such as rivers, and we also spent a great deal of effort on waste liquid treatment. Yes.

  In recent years, biological control, which is considered to have a smaller environmental impact than chemical pesticides, has attracted attention, and some have already been put into practical use. For example, microorganisms used for disease control of agricultural and horticultural plants include filamentous fungi belonging to the genus Trichoderma, Gliocladium, Talaromyces, bacteria belonging to the genus Agrobacterium, Pseudomonas and Bacillus.

However, the disease control of agricultural and horticultural plants by microorganisms is often not sufficiently effective. Therefore, attempts have been made to use microorganisms having an effect of controlling diseases of agricultural and horticultural plants together with chemical pesticides (for example, Non-patent Document 1, Patent Document 1, Patent Document 2, Patent Document 3, Patent Document 4, Patent Document). 5, Patent Document 6, Patent Document 7), which is effective for a wide range of control targets, and a combination of a microorganism and a chemical pesticide that has a higher synergistic effect when used in combination.
Syst, Fungic. Antifungal Compd. Vol. 2 291 pp. 257-267 DD267420A1 Publication JP-T 6-511258 JP-A-10-109913 JP 2005-512947 A JP 2005-29477 A JP 2006-96753 A Japanese Patent Application No. 2005-171785

  An object of the present invention is to provide an agricultural and horticultural composition that can obtain a high control effect even if the load on the environment is reduced by reducing the amount of chemical pesticide used, and is effective for a wide range of control targets. To do.

  As a result of intensive studies to solve the above problems, the present inventors can solve the above problems by using a spore of a bacterium belonging to the genus Bacillus together with a specific chemical pesticide and / or a natural pesticide. I found out. The present invention has been completed based on this finding.

The gist of the present invention is as follows.
(1) A composition for agricultural and horticultural use that contains bacterial spores belonging to the genus Bacillus and one or more agricultural and horticultural agricultural chemical components and has an action of controlling undesirable diseases of plants.
(2) The agricultural and horticultural composition according to (1), wherein the agricultural and horticultural agricultural chemical component is an agricultural and horticultural sterilizing component.
(3) The agricultural and horticultural composition according to (2), wherein the agricultural and horticultural bactericidal component is selected from a copper compound, mepronil, bench-avaricarb isopropyl, penthiopyrad, and polyoxin.
(4) The agricultural and horticultural composition according to (1), wherein the agricultural and horticultural agricultural chemical component is an agricultural and horticultural insecticidal component.
(5) The agricultural and horticultural composition according to (4), wherein the agricultural and horticultural insecticidal component is selected from BT agents, spinosad, milbemectin and imidacloprid.
(6) The agricultural and horticultural composition according to (4) or (5), which has an action of controlling harmful insects harmful to plants in addition to the action of controlling undesirable diseases of plants.
(7) The agricultural and horticultural composition according to (1), wherein the agricultural and horticultural agricultural chemical component is an agricultural and horticultural plant growth regulating component.
(8) The agricultural and horticultural composition according to (7), wherein the plant growth regulating component for agricultural and horticultural use is selected from prohexadione salt and trinexapac ethyl.
(9) In addition to the action of controlling undesired diseases of plants, it also has at least one action of regulating plant growth, grass shape, tree shape, flowering, and fruit setting (7) or (8) The composition for agricultural and horticultural use according to 1.
(10) The agricultural or horticultural composition according to any one of (1) to (9), wherein the bacterium belonging to the genus Bacillus is Bacillus subtilis.
(11) A bacterium belonging to the genus Bacillus is a Bacillus sp. The agricultural and horticultural composition according to any one of (1) to (9), which is D747 (accession number FERM BP-8234).
(12) A method for controlling plant diseases, comprising treating the plant or body with the composition for agriculture or horticulture according to any one of (1) to (11).
(13) A method for controlling pests, comprising treating a plant body with the composition for agriculture and horticulture according to any one of (4) to (6).
(14) At least one of plant growth, grass shape and tree shape, flowering, and fruiting, characterized by treating the plant or horticultural composition according to any one of (7) to (9) How to adjust.
(15) A plant disease characterized by suspending bacterial spores belonging to the genus Bacillus and one or more agricultural and horticultural agricultural chemical ingredients in water to form a mixed solution, and treating the mixed solution into a plant body Control method.
(16) A pest characterized by suspending a spore of a bacterium belonging to the genus Bacillus and one or more insecticidal components for agricultural and horticultural use in water to form a mixed solution, and treating the mixed solution into a plant body Control method.
(17) A spore of a bacterium belonging to the genus Bacillus and one or more kinds of plant growth regulating ingredients for agricultural and horticultural use are suspended in water to form a mixed solution, and the mixed solution is processed into a plant body. A method of adjusting at least one of plant growth, grass shape and tree shape, flowering, and fruit setting.

  The composition for agricultural and horticultural use according to the present invention has a high synergistic effect between microorganisms, which are active ingredients, and chemical pesticides and / or natural pesticides. As a result, the load on the environment can be reduced. Furthermore, the composition of the present invention containing an insecticidal component or a plant growth regulating component as an active ingredient can provide a pest controlling effect and a crop growth regulating effect as well as a disease controlling effect, thereby contributing to labor saving in farm work.

  The composition for agriculture and horticulture of the present invention contains spores obtained from a culture of bacteria belonging to the genus Bacillus as an active ingredient.

  Bacteria belonging to the genus Bacillus used in the present invention are not particularly limited as long as they belong to the genus Bacillus, but preferably include bacteria belonging to the genus Bacillus that antagonize phytopathogenic fungi, more preferably Bacillus subtilis and Bacillus sp. D747.

  This strain D747 is a strain isolated from the air in Kikukawa City, Shizuoka Prefecture, and is currently being used by the National Institute of Advanced Industrial Science and Technology, the Patent Biological Deposit Center as Bacillus sp. It is deposited internationally as D747 (accession number FERM BP-8234).

  This Bacillus sp. The bacterial properties of strain D747 are as shown below. The bacteriological properties were tested based on Bergey's Manual of Systematic Bacteriology, volume 1 (1984).

(A) Morphological properties Form: Neisseria gonorrhoeae Size: Width 1.0 to 1, 2 μm, Length 3 to 5 μm
Mobility: +
Flagellar state: Periflagellate Endospore: +
Spore position: center Spore expansion: −

(B) Culture properties Colony color: White to light brown Meat agar plate culture: White to creamy colonies, wrinkled surface

(C) Physiological properties Gram stainability: +
Reduction of nitrate: +
MR test: −
VP test: +
Indole production: −
Hydrolysis of starch: +
Utilization of citric acid: +
Inorganic nitrogen source: +
Oxidase: −
Catalase: +
Growth pH
6.8, meat extract medium: +
5.7, meat extract medium: +
Growth temperature 30 ° C: +
50 ° C .: −
Growth NaCl concentration 2%: +
5%: +
7%: +
Aerobic growth: +
Anaerobic growth: −
OF test: O
Egg yolk reaction: −
Acid production from glucose: +
Acid production from mannitol: −
Acid production from L-arabinose: −
Acid production from D-xylose: −
Gas production from glucose: −
β-galactosidase: −
NaCl and KCl requirements: −

  The composition for agricultural and horticultural use according to the present invention has a Bacillus sp. D747 is contained. Although the D747 strain can be used alone in the agricultural and horticultural composition of the present invention, a mutant of the D747 strain can also be used alone or together with the D747 strain. The mutant has the bacteriological characteristics of the above-mentioned D747 strain and has a plant disease control action, and is a natural mutant, a mutant using ultraviolet rays or chemical mutants, a cell fusion strain and a gene. Recombinant strains can also be used. The D747 strain contained in the agricultural and horticultural composition of the present invention includes a mutant of the D747 strain.

  The spore used in the present invention is obtained from a culture of bacteria belonging to the genus Bacillus. Bacteria belonging to the genus Bacillus can be cultured according to the usual culture method for bacteria belonging to the genus Bacillus, such as reciprocal shaking culture, jar fermenter culture, liquid culture such as a culture tank, and solid culture. For example, in addition to a common medium such as a meat extract medium, a medium containing glucose, peptone, yeast extract, and the like can be mentioned. In addition to the liquid medium, a solid medium such as a slope medium containing agar and a plate medium may be used. The D747 strain can be grown by culturing to obtain the desired cell mass.

  Any carbon source that can be assimilated by the above strain can be used as the carbon source of the medium. For example, in addition to sugars such as glucose, galactose, lactose, sucrose, maltose, malt extract, and starch hydrolysate, various synthetic or natural carbon sources that can be used by the strain D747 can be mentioned. Similarly, as a nitrogen source of the medium, various synthetic or natural products that can be used by the strain, including organic nitrogen-containing materials such as peptone, meat extract, and yeast extract, can be used. In accordance with a conventional method for culturing microorganisms, inorganic salts such as sodium chloride and phosphate, metal salts such as calcium, magnesium and iron, trace nutrients such as vitamins and amino acids can be added as necessary.

  The culture can be performed under aerobic conditions such as shaking culture and aeration culture. The culture temperature is 20-30 ° C., preferably 25-30 ° C., pH 5-8, preferably 6-7, and the culture period is 1-4 days, preferably 2-3 days.

  As a method for separating spores from a culture of bacteria belonging to the genus Bacillus obtained as described above, a method such as membrane separation, centrifugation, or filtration separation can be used. The obtained spore fraction can be used as it is for the agricultural or horticultural composition of the present invention in a state of containing a certain amount of water, or a dried product using a drying method such as freeze-drying or spray-drying as necessary. It is also possible to use this for the agricultural and horticultural composition of the present invention.

  The present invention newly found that the plant disease control action by spores derived from Bacillus bacteria is synergistically enhanced by the combined use with agricultural and horticultural agrochemical ingredients, and has been completed based on this useful new knowledge. .

  In the present invention, when the agricultural and horticultural agrochemical component is an agricultural and horticultural sterilizing component, not only is it excellent in synergistically enhancing the sterilizing effect, but also unexpectedly, It has been found for the first time that bactericidal action is synergistically enhanced even when bacterial spore is used in combination with agricultural horticultural insecticidal ingredients or agricultural and horticultural plant growth control ingredients, which are not or have very low agricultural and horticultural agricultural chemical ingredients. In that respect, it is very new, unique and outstanding. It is very innovative and characteristic that the action effect of the fungicide is synergistically enhanced by the combined use with insecticides and / or plant growth regulators.

  Therefore, the present invention further includes an invention relating to an agricultural and horticultural composition having the following plant disease control action. That is, the spore of the genus Bacillus is (A), the agricultural and horticultural agricultural chemical component is (B), and among (B), the agricultural and horticultural sterilizing component, the insecticidal component, and the plant growth regulating component are (B -1), (B-2), and (B-3), the present invention also provides the following agricultural and horticultural compositions for controlling plant diseases.

I: (A) + (B)
II: (A) + (B-1)
(A) + (B-2)
(A) + (B-3)
III: (A) + (B-1) + (B-2)
(A) + (B-1) + (B-3)
(A) + (B-2) + (B-3)
(A) + (B-1) + (B-2) + (B-3)

  In addition, when an insecticidal component or a plant growth regulating component is used as an agrochemical component, an insecticidal action or a plant growth regulating action is exhibited in addition to a plant disease controlling action.

  The agricultural and horticultural agrochemical component used in the present invention provides a synergistic control effect when used in combination with bacterial spores belonging to the genus Bacillus. These agricultural and horticultural pesticide ingredients are commercially available or are known as agricultural and horticultural pesticides. These ingredients are the Agricultural Handbook published by the Japanese Plant Protection Association (2002), and the Agricultural Handbook published by the Japanese Plant Protection Association. (2005), Kumiai Agricultural Chemicals Directory published by the National Federation of Agricultural Cooperatives (2005) and SHIBUYA INDEX (2005) published by the Federation.

  Examples of agricultural and horticultural agricultural chemical components that can be used include bactericidal components, insecticidal components, acaricidal components, nematicidal components, herbicidal components, plant growth regulating components, and the like. Specific examples of the bactericidal component include, for example, acibenzoral S methyl, azoxystrobin, amisulbrom, isotianil, isoprothiolane, ipconazole, iprodione, iprovalicarb, iprobenfos, iminoctazine albesylate, iminoctadine acetate, imibenconazole, echromomezole, edifenphos, Ethaboxam, edifenphos, oxadixyl, oxytetracycline, oxpoconazole fumarate, oxolinic acid, orissastrobin, kasugamycin, captahol, carpropamide, carbendazole, quinoxyphene, quinomethionate, captan, quintozene, guazatine, cresoxime methyl, chloronecidomil, azolotamidoyl , Diethofencarb, Diclocimet, Di Lomedin, dithianon, dinebu, difenoconazole, cyflufenamide, diflumetrim, cyproconazole, cyprozinyl, simeconazole, dimethomorph, simoxanyl, dimoxystrobin, ziram, streptomycin, zoxamide, dazomet, thiadiazine, thiazinyl, thiabendioazole, thiuramthium flutethramate , Tetraconazole, tebuconazole, triadimephone, tricyclazole, triflumizole, trifloxystrobin, trifolin, tolcrofosmethyl, torniphanide, validamycin, picooxystrobin, viteltanol, hydroxyisoxazole, himexazole, pyrifenox, pyributycarb , Pyrimethanil, pyrokilo , Viroclostrobin, vinclozolin, famoxadone, phenazine oxide, phenamidone, fenamimol, phenoxanyl, ferrimzone, fenbuconazole, fenhexamide, folpamide, fosulfide, blasticidin S, flametopir, fluazinam, fluoxastrobin, flupicolide, fluorimide , Fludioxonil, Fursulfamide, Flutolanil, Proquinazide, Prochloraz, Procymidone, Propamocarb hydrochloride, Propiconazole, Propineb, Probenazole, Hexaconazole, Benomyl, Pefrazoate, Pencyclon, Benchavalicarb isopropyl, Benchopirad, Boscalido, Polyoxine, Polyoxine Carbamate, Holpet, Mandipropamide, Manzeb, Mannebu , Microbutanyl, metalaxyl, metminostrobin, metolaphenone, mepanipyrim, mefenoxam, mepronil, silver, inorganic copper compound, organic copper compound, sulfur compound, organic zinc, potassium bicarbonate, sodium bicarbonate, fatty acid glyceride, shiitake mycelium extract, The microbial pesticides selected from the genus Erbinia, Pseudomonas, Bacillus, Talaromyces, Trichoderma, and Fusarium can be used. Examples of the copper compound include inorganic copper compounds such as basic copper sulfate, anhydrous copper sulfate, cupric hydroxide, basic copper chloride, and organic copper compounds such as organic copper and nonylphenol sulfonate copper. be able to.

  Specific examples of the insecticidal component, the acaricidal component and the nematicidal component include, for example, 1.3 dichloropropene (1.3-D), BPMC, BPPS, BRP, CL900187, cryolite, CVMP, CYAP, DCIP, DD , DCIP, DDVP, DEP, DMTP, ECP, EPN, MEP, MIPC, MPP, NAC, N-methyldithiocarbamate ammonium (NCS), NNI-0101, PAP, PHC, RU15525, XDE-175, XMC, ZXI8901, Acrinatrin , Azamethifos, azinephos-ethyl, azinephos-methyl, acequinosyl, acetamiprid, acetoprole, acephate, azocyclotin, abamectin, amitraz, alanibalbu, aldicarb, alpha-cypermethrin, allethrin, i Carbophos, isoxathione, isoprocarb, imiciaphos, imidacloprid, imiprothrin, indoxacarb, esfenvalerate, etiophencarb, ethion, ethiprole, ethylthiomethone, etoxazole, etofenprox, ethoprophos, emamectin, endosulfane, pentometline, oxymetholone , Sodium oleate, carbam, carbam sodium, kazusafos, cadreslin, cartap, carbaryl, carbosulfan, carbofuran, gamma cyhalothrin, xylylcarb, quinalphos, quinoprene, quinomethionate, coumaphos, clothianidin, clofentezin, chromaphenoid, chlor Ethoxyphos, chlordane, chloropicrin Chlorpyrifos, Chlorpyrifos-methyl, Chlorfenapyr, Chlofentezine, Chlorfenvinphos, Chlorfluazuron, Chromafenozide, Chlormefos, Sanopyrafene, Cyanophos, Diafentiuron, Dienochlor, Dicrotophos, Cycloprotolin, Dichlorvos, Disulfotone, Dinotefuran , Ciphenothrin, cifluthrin, diflubenzuron, cyflumethofene, cyhexatin, cypermethrin, dimethylvinphos, dimethoate, silafluophene, cyromazine, spinosad, spirodiclofen, spirotetramat (spirotetramat), spiromesifen, sulprophos, sulfotep, zeta Cypermethrin, diazinon, taufulvalinate, thiacloprid, thiamethoxam, thio Dicarb, thiocyclam, thiosultap, thiophanox, thiometone, tetrachlorvinphos, tetradiphone, tetramethrin, tebupyrimfos, tebufenozide, tebufenpyrad, tefluthrin, teflubenzuron, demeton S-methyl, temefos, delice, deltamethrin, terbufos, tralomethrin, transtromethrin , Triazamate, triazophos, trichlorfone, triflumuron, trimetacarb, tolfenpyrad, sodium = methyldithiocarbamate, nared, nitenpyram, nemadectin, novallon, nobiflumuron, hydroprene, bamidthione, parathion, parathion methyl, halfenprox, halophenozrine, bioalethrin, bioalethrin , Bistrifluron, Pidaf Nthione, bifenazate, bifenthrin, pymetrozine, pyracrofos, pyridafenthion, pyridaben, pyridalyl, pyriproxyfen, pyrimicarb, pyrimidifen, pyrimiphosmethyl, pyrethrin, famflu, fipronil, phenazaquin, phenamiphos, phenisobromolate, fenitrothion, phenoxycarb, phenoxycarb, phenoxycarb Fenobucarb, fenthion, phentoate, fenvalerate, fenpyroximate, fenbutantin oxide, fenpropatoline, butcarboxyme, butoxycarboxyme, buprofezin, furthiocarb, praletrin, fluacrylpyrim, flucycloxuron, flucitrinate, flusulfamide, fulvalinate , Flupyrazophos Flufenoxuron, fulvendiamide, flumethrin, flurimfen (flufenem), prothiophos, flonicamid, propaphos, propixur, profenofos, propetamphos, bromopropyrate, beta-cypermethrin, beta cyfluthrin, hexaflumuron, hexithiazox, heptenophos, Permethrin, bensultap, benzoepin, bendiocarb, benfuracarb, phoxime, hosalone, phosthiazete, phosphamidone, phosmet, formethanate, folate, machine oil, malathion, milbemectin, mecarbam, mesulfenphos, mesomil, metaldehyde, metaflumizone, methamidophos, methicarb, methicarb Methyl isothiocyanate, methoxy phenozide, methotri , Methoprene, metorcarb, mevinphos, monocrotophos, lambda cyhalothrin, linaxyl, lufenuron, resmethrin, repimectin, roteno, propylene glycol monofatty acid ester, nicotine sulfate, levamisole hydrochloride, ethylene oxide, fenbutastin oxide, fatty acid glyceride, morantel tartrate, rapeseed oil , Starch, soybean lecithin, BT, Verticillium regani, Pasteuria penetrance can be used. The BT agent as used herein is a general term for agricultural chemicals using the bacteria Bacillus thuringiensis, and includes crystal proteins produced by bacteria, viable spore, and a mixture of both, and any of them can be used in the present invention.

  Specific examples of herbicidal components include, for example, 2,3,6-TBA, 2,4-D, 2,4-DB, 2,4-PA, ACN, CAT, DBN, DCBN, DCMU, DCPA, DNOC, DPA , EPTC, HC-252, IPC, KIH-485, MCPA, MCPA, isopropylamine salt, MCPA, ethyl, MCPA, sodium, MCPA, thioethyl, MCPB, MCPP, MDBA, MDBA, isopropylamine salt, MDBA, sodium salt, PAC, SAP, S-metolachlor, TCTP, TM-435, aioxynil, acronifene, acifluorfen, azimusulfuron, ashram, acetochlor, atrazine, anilophos, amicarbazone, amidosulfuron, amitrol, aminopyralide, amiprophosmethyl Ametrine, alachlor, aloxidim, ansimidol, iodosulfuron, isouron, isoxaflutol, isoxaben, isoproturon, imazaquin, imazapyr, imazametabenz, imazametapyr (imazapic), imazamox, imazetapyrone, imodasulfuron, prodruganesulfone・ Methyl, ethalfluralin, ethoxysulfuron, etofumesate, ettobenzanide, disodium endal, oxadiazone, oxadiargyl, oxadiclomephone, oxasulfuron, oxyfluorfen, oryzalin, olbencarb, fenfentrol, carfentrazone-ethyl, carbbutylate, carbetamide, Quizarohop P, Quizarohop P Eth, Quizaro P.P.Tefryl, Quizalofop-Ethyl, Quinoclamine, Quincolac, Kinmerac, Cumyllon, Glyphosate, Glyphosate / Ammonium Salt, Glyphosate / Isopropylamine Salt, Glyphosate / Potassium Salt, Glyphosate / Trimesium Salt (Sulfosate) Salt, glufosinate, glufosinate sodium salt, cretodim, clodinahop, clopyralid, clomazone, clomeprop, chloransram methyl, chloridazon, chlorimuron ethyl, chlorsulfuron, chlortal dimethyl, chlorphthalim, chlorprofam, chlormecort, chlorotoluron, cyanazine, diuron, Dicamba, cycloate, cycloxydim, diclosram, cyclosulfamuron, diclobeni , Diclohop methyl, dichloroprop, dichlorprop-P, diquat, dithiopyr, ciduron, dinitramine, sinidone ethyl, sinosulfurone, dinoterb, cyhalohop butyl, diphenamide, diflufenican, diflufenzopyr, diflumetrim, simazine, dimethachlor , Dimetamethrin, dimethenamide, cimethrin, dimethylpiperate, dimeflon, symmetrine, sulcotrione, sulfentrazone, sulfosulfuron, sulfomethuron-methyl, cetoxydim, terbacil, dimeron, thiazopyr, thiencarbazone, thiocarbazyl, thiobencarb, thiazulone, thifensulfur , Decyl alcohol, desmedifam, tetrapion, tenyl chlor, tebuthiuron, tep Roxydim, tefriltrione, terbutyrazine, terbutridine, terbumethone, tembotrione, topramison, tolalkoxydim, triadifram, triasulfuron, triarate, trietadine, triclopyr, trisulfuron methyl, tritosulfuron, trifluralin, trifloxysulfuron sodium salt, tribenuron Methyl, naptalam, naproanilide, napropamide, nicosulfuron, nebulon, norflurazon, paraquat, haloxyhop, haloxyhop-P, haloxyhop-P-methyl, halosulfuron-methyl, bialaphos, pixaden, picloram, picolinaphene, bispyribak sodium salt, bifenox, Pyraclonil, pyrasulfotol, pyrazoxifene, pyrazosulfuro・ Ethyl, pyrazolinate (pyrazolate), vilanaphos, pyraflufen ・ ethyl, pyrithiobac ・ sodium salt, pyridate, pyriftalide, pyributycarb, pyribenzoxime, pyrimisulfane, pyrimisulfuron methyl, pyriminobac methyl, phenoxaprop P. Phenoxaprop-ethyl, fenchlorim, fentrazamide, fenmedifam, foramsulfuron, butachlor, butaphenacyl, butamifos, butyrate, butralin, butroxidim, flazasulfuron, framprop-M, fluazifop, fluazifop-P, fluometuron, fluoroglycophene , Flucarbazone sodium salt, flucetosulfuron, fluthiaset methyl, flupirsulfuron, flufena Set, flupoxam, flumioxazin, full microlac pentyl, flumeturum, flurprimidol, fluroxypyr, pretilachlor, prodiamine, prosulfuron, prosulfocarb, propoxahop, propachlor, propazine, propanil, propizzamide, propisochlor, Profam, propoxycarbazone, propoxycarbazone sodium salt, propoxydim, bromacil, promethrin, prometon, bromoxynyl, bromobutide, florasulam, hexazinone, bethrosin, petoxamide, benazoline, penoxsulam, pebrate, benzphendizone, benzulide, bensulfuron methyl , Benzobicyclon, benzophenap, bentazone, pentanochlor, bencho curve, pende Metalin, pentoxazone, benfluralin, benfrate, fomesafen, forchlorfenuron, maleic acid hydrazide, mecoprop, mecoprop-P.potassium salt, mesosulfuron methyl, mesotrione, metazachlor, metabenzthiazulone, metamitron, metamihop, methyldimuron , Metoxuron, Methoslam, Methosulfuron / Methyl, Metobenzuron, Metolachlor, Metribuzin, Mepicoat Chloride, Mefenacet, Monolinuron, Molinate, Lactofen, Linuron, Rimsulfuron, Renacyl, Zantomonas campestris, Drexerella monoceras it can.

  Specific examples of plant growth regulating components include, for example, abscisic acid, inabenfide, indole, uniconazole P, ethiclozeate, etephone, oxine sulfate, calcium formate, cloxiphonac, chlormequat, choline, cyanamide, dichlorprop, gibberellin, daminozide, Decyl alcohol, butoruraline, trinexapac ethyl, paclobutrazole, paraffin, pyraflufenethyl, butorarin, flurprimidol, prohydrojasmon, prohexadione calcium salt, benzylaminopurine, pendimethalin, forchlorfenu Ron, maleic hydrazide, mepiquat chloride, wax, MCPA / thioethyl, MCPB, 1-naphthylacetamide, 4-CPA, calcium chloride, calcium sulfate , Calcium peroxide, chlorella extract, those selected from mixture herbal extract can be used.

  The agricultural and horticultural composition of the present invention is prepared by adding a spore of the bacterium belonging to the genus Bacillus and the agricultural and horticultural agrochemical component according to a normal method for producing a microbial preparation, and adding a surfactant and other auxiliary agents as necessary It may be used as a preparation. Specific examples of preparations include dosage forms such as granules, powders, wettable powders, suspension preparations, emulsions, and coating agents. Examples of preferred carriers include solid carriers such as talc, bentonite, clay, kaolin, diatomaceous earth, white carbon, vermiculite, slaked lime, silica sand, ammonium sulfate, urea, porous, water, isopropyl alcohol, xylene, cyclohexanone, methylnaphthalene, alkylene Examples thereof include liquid carriers such as glycol. Examples of the surfactant and dispersant include dinaphthyl methane sulfonate, alcohol sulfate ester salt, alkyl aryl sulfonate, lignin sulfonate, polyoxyethylene glycol ether, polyoxyethylene alkyl aryl ether, and polyoxyethylene sorbitan. Examples include monoalkylates. Examples of the auxiliary agent include carboxymethyl cellulose, polyethylene glycol, propylene glycol, gum arabic, and xanthan gum, and examples of the protective agent include skim milk and pH buffering agent. In this case, the amount of the D747 strain and / or its culture, as well as the application time and application amount, can be appropriately determined according to the case of the above live bacteria.

  The composition for agricultural and horticultural use of the present invention thus obtained can be stored for a long period of time regardless of the form of the preparation. For example, after being stored as a wettable powder at room temperature for 48 months, A control value equivalent to that without the addition of the agrochemical component can be obtained.

  The composition for agricultural and horticultural use according to the present invention, depending on the application form, fungi belonging to Algaemycetes, Ascomycetes, Basidiomycetes, Zygomycetes, and Deuteromycetes, And plant diseases caused by bacteria can be controlled.

  Specific examples of plant pathogens that can be controlled by the agricultural and horticultural compositions of the present invention include Pseudoperonospora, such as Pseudoperonospora cubensis and Venturia. For example, Venturia inaequalis, Erysiphe genus, such as wheat powdery mildew (Erysiphegraminis), Pyricularia bacterium, for example, Pyricularia borax Botrytis cinerea, Rhizoctonia spp. For example, Rhizoctonia solani, Cladosporium genus, such as Tomato leaf mold (Cladosporum fulvum), Colletotrichum genus, eg, Strawberry anthracnose (Colletotrichum pulmonica) ) Genus bacteria such as wheat red rust fungus (Puccinia recondita), Septoria genus bacteria such as wheat blight fungus (Septoria nodorum), genus Sclerotinia (eg, Sclerotini sclerotia sclerotia) (Pythium) genus, for example, cucumber seedling Bacterial fungi (Pythium debaryanum Hesse), genus Gaeumanomyces, such as wheat blight fungus (Gaemannnmyces graminis), and bacteria such as Burkholderia genus, bacteria such as B. However, the present invention is not limited to these examples.

  In addition, the plant pests that can be controlled by the agricultural and horticultural composition of the present invention, which include insecticide components, are specifically Ayayotou, Rice Butter, Phterophyllum yam, Tamanayaga, Cottonback Kiriba, Green Tobacco moth, White-bellied Tomato, Spodoptera litura, Kabrayaga, Mushrooms Lepidopterous insect pests such as Skashiba, Chanohosuga, Koagaga, Oiga; Tobacco whitefly, Onsitsunajiri, Mikantogekonajirami, Cottonfly Mussels, Snowy Venus, Ringworm Aphid, Japanese Aphid Aphid, Falcon Aphid, Bean Aphid, Komikan Aphid, Citrus Aphid, Grape Aphid, Barley Aphid, Potato Aphid, Chanomidori Himebai Leafhopper, Himetobunka, Flying planthopper, White-footed planthopper, White-footed leafhopper, Thai one-winged leafhopper, White-footed leafhopper, Ruby-worm, Olive-caterpillar, San-house scale insect, Apple-spotted scale, Red-bellied scale, Agaric scale Iceria scale insects, apple whales, citrus whales, minamiaoka Hemiptera pests such as worms, beetle beetles, nasigunbui; Crustacea insects such as larvae, spider mites, spider mites, tick worms, mites However, the present invention is not limited to these examples.

  Furthermore, the composition containing the plant growth regulating component in the composition for agricultural and horticultural use of the present invention, for example, adjusting the growth of the plant, changing the grass shape or the tree shape to a more preferable one, adjusting the flowering or fruit setting, Although the promotion of rooting of crops, growth promotion, fruit enlargement, seedlessness, promotion of flowering, or reduction of crop height and shaping, prevention of lodging, etc. can be mentioned. It is not limited by the examples.

  Examples of crops to which the agricultural and horticultural composition of the present invention is applied include grains (eg, rice, wheat, barley, corn, buckwheat), potatoes (eg, potato, sweet potato, taro, yam), legumes (eg, Soybeans, green beans, azuki beans, peas), vegetables (for example, cucumbers, tomatoes, eggplant, peppers, cabbage, Chinese cabbage, radish, lettuce, carrots, leeks, onions, strawberries, spinach, celery), fruit trees (for example, apples, Pear, sugarcane, peach, grape, oyster, citrus, kiwi), special crop (eg cotton, rapeseed, sunflower, beet, sugarcane, tobacco), turf, tree, ornamental plant (eg rose, chrysanthemum, tulip, And the like, but the present invention is limited by these examples. Not. Moreover, the composition for agricultural and horticultural use of the present invention can be similarly used for crops transformed by a technique such as genetic recombination.

  The agricultural and horticultural composition of the present invention can be applied directly as it is, or diluted with water or the like. When applying directly as it is, a method of spraying the powder directly on the plant, a method of applying the coating agent to a part of the plant, a method of spraying the powder or granule on the soil, a method of mixing the powder or granule in the soil, a powder Powdered seeds and tubers, a method of smearing suspension preparations on seeds and tubers, a method of spraying granules and suspension preparations in paddy field water, and the like. is not. When diluting with water, etc., a wettable powder, granule, suspension formulation, emulsion, etc. are diluted with water to a concentration suitable for use, and the suspension is sprayed onto the plant. Application method, a method of spraying on the soil, a method of irrigating the soil, a method of smearing on seeds and tubers, a method of spraying in the paddy field water, etc. Absent. In addition, a spreading agent, a fertilizer, and other agricultural chemicals may be added to the water diluted to apply the agricultural and horticultural composition of the present invention, if necessary. Furthermore, the composition for agricultural and horticultural use of the present invention is mixed, applied alternately or simultaneously with other fungicides, insecticides, nematicides, herbicides, plant growth regulators, fertilizers, soil improvement materials and the like. It is also possible.

The application amount of the composition for agricultural and horticultural use according to the present invention varies depending on the type of pest, the type of applied plant, the dosage form, etc., and thus cannot be specified in general. For example, when spraying a suspension diluted with water or the like. Is a spore concentration of 10 ⁵ to 10 ¹⁰ cfu (colony forming unit) / ml, preferably 10 ⁷ to 10 ⁹ cfu / ml. The application rate is preferably 0.5 to 1000 liters of suspension per 10 ares.

  The present invention will be specifically described by the following examples, but the present invention is not limited to these examples.

(Production Example 1: Production of wet cells)
Bacillus sp. Isolated from the air in Shizuoka Prefecture. D747 strain (FERM BP-8234) was cultured on a plate medium, and the isolated colonies were inoculated into a flask. In a 20 ml broth medium (meat extract 1%, peptone 1%, sodium chloride 0.5%), 27 C., 120 rpm, and shaking culture for 1 day, the resulting culture solution was glucose 1%, soluble starch 2%, polypeptone 0.5%, dry yeast 1%, defatted soybean 1%, KH ₂ PO ₄ 0.2%, After inoculating 20 L of a pH 6.0 medium containing 0.2% sodium chloride and 0.3% calcium carbonate, and shaking culture at 27 ° C., 120 rpm for 3 days, centrifugal collection (10,000 × g, 15 And suspended in sterile water to wash the media components. This operation was performed twice to obtain wet cells (spore fraction) having a wet weight of about 1 kg. This spore fraction is a Bacillus sp. It contains 50% by weight of D747 spores by dry weight.

(Production Example 2: Production of powder spore)
The Bacillus sp. 1 kg of the D747 spore fraction was suspended in 5 L of distilled water and then treated with a spray dryer (Niro Japan) (inlet temperature 150 ° C., outlet temperature 100 ° C.). By crushing the dried product obtained by the spray dryer, about 100 g of dry powder spores were obtained. This dry powder is Bacillus sp. D747 spores containing 6 × 10 ¹¹ cfu / g or more.

  Next, the preparation method will be specifically described with reference to typical preparation examples of the agricultural and horticultural composition of the present invention. In the following description, “%” indicates a weight percentage.

(Formulation example 1: wettable powder)
Bacillus sp. Obtained as described above. Powder spores of D747 strain 3.3%, agricultural and horticultural fungicide polyoxin complex 1.0%, naphthalenesulfonic acid formalin condensate sodium salt 1.5%, polyoxyethylene alkylaryl 1.5%, diatomaceous earth 26 % And clay 67.7% were mixed and ground uniformly to obtain a wettable powder.

(Formulation example 2: wettable powder)
Bacillus sp. Obtained as described above. 6.7% powder spores of D747 strain, 1% of agricultural and horticultural bactericidal compound penthiopyrad, naphthalenesulfonic acid formalin condensate sodium salt 1.5%, polyoxyethylene alkylaryl 1.5%, diatomaceous earth 26%, clay 63 .3% was uniformly mixed and ground to obtain a wettable powder.

(Formulation example 3: wettable powder)
Bacillus sp. Obtained as described above. D747 strain powder spores 13%, agricultural and horticultural insecticide spinosad 20%, naphthalenesulfonic acid formalin condensate sodium salt 1.5%, polyoxyethylene alkylaryl 1.5%, diatomaceous earth 26%, clay 38% Uniformly mixed and ground to obtain a wettable powder.

(Formulation example 4: wettable powder)
Bacillus sp. Obtained as described above. 10% powder spore of D747 strain, 10% viable spore of agricultural and horticultural insecticide, 1.5% naphthalenesulfonic acid formalin condensate sodium salt, 1.5% polyoxyethylene alkylaryl, 26% diatomaceous earth, clay 51% was uniformly mixed and ground to obtain a wettable powder.

(Formulation example 5: wettable powder)
Bacillus sp. Obtained as described above. Powder spore of D747 strain 3.3%, plant growth regulating compound prohexadione calcium salt for agriculture and horticulture 5%, naphthalenesulfonic acid formalin condensate sodium salt 1.5%, polyoxyethylene alkylaryl 1.5%, 26% diatomaceous earth and 62.7% clay were mixed and ground uniformly to obtain a wettable powder.

(Formulation example 6: wettable powder)
Bacillus sp. Obtained as described above. 3.3% powder spore of D747 strain, 5% plant growth regulating compound trinexapac ethyl for agricultural and horticultural use, 1.5% naphthalenesulfonic acid formalin condensate sodium salt, 1.5% polyoxyethylene alkylaryl, diatomaceous earth 26% and 62.7% of clay were mixed and ground uniformly to obtain a wettable powder.

(Formulation example 7: wettable powder)
Bacillus sp. Obtained as described above. 10% powder spore of D747 strain, 1% milbemectin for agricultural and horticultural insecticide, 1.5% naphthalenesulfonic acid formalin condensate sodium salt, 1.5% polyoxyethylene alkylaryl, 26% diatomaceous earth, 60% clay Uniformly mixed and ground to obtain a wettable powder.

(Formulation Example 8: Powder)
Bacillus sp. Obtained as described above. The powder spore of D747 strain was uniformly mixed and pulverized with 10% of the powder spore, 1% of the agricultural and horticultural fungicide mepronil, 6% of diatomaceous earth, and 83% of clay to obtain a powder.

(Formulation Example 9: Granules)
Bacillus sp. Obtained as described above. 10% powder spores of D747 strain, 1% agricultural and horticultural insecticide compound imidacloprid, 2% sodium lauryl alcohol sulfate, 5% sodium lignin sulfonate, 2% carboxymethylcellulose and 80% clay were mixed and ground uniformly. . 20 parts by weight of water was added to 100 parts by weight of the mixture, kneaded, processed into granules of 14 to 32 mesh using an extrusion granulator, and dried to form granules.

(Formulation example 10: wettable powder)
Bacillus sp. Obtained as described above. 10% powder spore of D747 strain, 1.5% naphthalenesulfonic acid formalin condensate sodium salt, 1.5% polyoxyethylene alkylaryl, 26% diatomaceous earth, 61% clay, and uniformly pulverized, wettable powder It was.

(Formulation example 11: wettable powder)
Agricultural and horticultural bactericidal substance polyoxin complex 1.0%, naphthalene sulfonic acid formalin condensate sodium salt 1.5%, polyoxyethylene alkylaryl 1.5%, diatomaceous earth 26%, clay 70% uniformly mixed and ground. To obtain a wettable powder.

(Formulation example 12; wettable powder)
Agricultural and horticultural bactericidal compound, penthiopyrad 1%, naphthalene sulfonic acid formalin condensate sodium salt 1.5%, polyoxyethylene alkylaryl 1.5%, diatomaceous earth 26%, clay 70% are uniformly mixed and ground to hydrate An agent was used.

(Formulation example 13: wettable powder)
Agricultural and horticultural insecticide Spinosad 20%, Naphthalenesulfonic acid formalin condensate sodium salt 1.5%, polyoxyethylene alkylaryl 1.5%, diatomaceous earth 26%, clay 51% are uniformly mixed and pulverized. An agent was used.

(Formulation example 14: wettable powder)
Agricultural and horticultural insecticide BT viable spore 10%, naphthalenesulfonic acid formalin condensate sodium salt 1.5%, polyoxyethylene alkylaryl 1.5%, diatomaceous earth 26%, clay 61% A wettable powder.

(Formulation Example 15: wettable powder)
Agro-horticultural plant growth regulating compound Prohexadione calcium salt 5%, Naphthalenesulfonic acid formalin condensate sodium salt 1.5%, polyoxyethylene alkylaryl 1.5%, diatomaceous earth 26%, clay 66% uniformly mixed This was pulverized into a wettable powder.

(Formulation Example 16: wettable powder)
Agricultural and horticultural plant growth regulating compound trinexapac ethyl 5%, naphthalenesulfonic acid formalin condensate sodium salt 1.5%, polyoxyethylene alkylaryl 1.5%, diatomaceous earth 26%, clay 66% uniformly mixed and ground Thus, a wettable powder was obtained.

(Formulation Example 17: wettable powder)
Agricultural and horticultural insecticide Milbemectin 1%, Naphthalenesulfonic acid formalin condensate sodium salt 1.5%, Polyoxyethylene alkylaryl 1.5%, Diatomaceous earth 26%, Clay 70% are mixed and ground uniformly and hydrated An agent was used.

(Formulation Example 18: Powder)
An agricultural and horticultural bactericidal compound mepronil 1%, diatomaceous earth 6%, and clay 93% were uniformly mixed and ground to obtain a powder.

(Formulation example 19: granule)
Agricultural and horticultural insecticidal compound imidacloprid 1%, sodium lauryl alcohol sulfate 2%, sodium lignin sulfonate 5%, carboxymethyl cellulose 2% and clay 90% were uniformly mixed and ground. 20 parts by weight of water was added to 100 parts by weight of the mixture, kneaded, processed into granules of 14 to 32 mesh using an extrusion granulator, and dried to form granules.

  Next, test examples show that the agricultural and horticultural composition of the present invention is useful as a plant disease control agent.

(Test Example 1: Cucumber gray mold control effect test)
Ten cucumber seeds (variety: Sagamihanjiro) were sown in 9 cm x 9 cm PVC pots, grown in a greenhouse for 7 days, and cucumber seedlings with cotyledons were used as test plants. The composition for agricultural and horticultural use of the present invention prepared by appropriately changing the blending amount so as to obtain the test concentration according to Formulation Examples 1 to 7, Bacillus sp. D747 and the agricultural and horticultural fungicide, agricultural and horticultural insecticide, and agricultural and horticultural plant growth regulator prepared according to Formulation Examples 11 to 17 were diluted with water so that the active ingredient had a predetermined concentration. 10 ml per pot was sprayed. After air drying, a paper disc soaked with a spore suspension of Botrytis cinerea was placed on the surface of the cucumber cotyledon and maintained in a wet chamber at 20 ° C. Three days after the inoculation, the lesion diameter of the cotyledon was obtained, and the control value (%) was obtained from Equation 1 based on the obtained numerical value.

  The control effect expected when two types of active compounds and microorganisms are mixed and treated can be obtained from the calculation formula of Colby (calculation of synergistic and antagonistic responses of herbicide combinations, calculating) Synergistic and Antagonistic Responses of Herbicide Combination, Weed 15, 20-22, 1967). The calculation formula of Colby is shown in Formula 2.

  In Formula 2, X represents the control effect (control value) when one active compound or microorganism is treated at a concentration of m (ppm or cfu / ml), and Y represents the other active compound or microorganism n (ppm or The control effect (control value) when treated at a concentration of cfu / ml). In that case, E is the control when the former active compound or microorganism is mixed and treated to a concentration of m (ppm or cfu / ml) and the latter active compound or microorganism to a concentration of n (ppm or cfu / ml). It represents the expected value due to the additive effect (control value).

  The control effect of the agricultural and horticultural composition of the present invention is as follows. The control effect obtained by treating only D747, and the control effect obtained by treating only the agricultural and horticultural fungicide, insecticide and plant growth regulator as Y is larger than the calculated value (E) obtained from the calculation formula of Colby. If it becomes, it will show that the control effect by this combination is exhibited by the synergistic effect. Table 1 shows the control value (%) of the experimental value obtained by the test and the control value (%) of the calculated value obtained from the calculation formula of Colby.

Note) Pesticide component (A) Polyoxin complex (B): Penthiopyrad (C); Bench varicarb isopropyl (D): Cupric hydroxide (E): Spinosad (F): BT raw Fungal spores (G): Prohexadione calcium salt (H): Trinexapac ethyl (I): Milbemectin (J): Mepronil (K): Imidacloprid

(Test Example 2: Rice Blast Control Effect Test)
About 15 seeds of rice seed (variety: Aichi Asahi) were sown in 7 cm diameter clay pots, grown in a greenhouse for 2 to 3 weeks, and rice seedlings in which the fourth leaf was completely developed were used as test plants. The composition for agricultural and horticultural use of the present invention prepared by appropriately changing the blending amount so as to obtain the test concentration according to Formulation Examples 1 to 7, Bacillus sp. D747 and the agricultural and horticultural fungicide, agricultural and horticultural insecticide, and agricultural and horticultural plant growth regulator prepared according to Formulation Examples 11 to 17 were diluted with water so that the active ingredient had a predetermined concentration. 10 ml per pot was sprayed. After air-drying, a conidial spore suspension of Pyricularia oryzae was spray-inoculated and immediately placed in a humid chamber at 25 ° C. for 24 hours. Then, it moved to the greenhouse, and the number of lesions on the 4th leaf was investigated 5 days after the inoculation. Table 2 shows the control value (%) of the experimental value obtained by the test and the control value (%) of the calculated value obtained from the calculation formula of Colby.

Note) Pesticide component (A): polyoxin complex (B): pentiopyrad (C): benchavalicarbisopropyl (D): cupric hydroxide (E): spinosad (F): BT Viable spore (G): Prohexadione calcium salt (H): Trinexapac ethyl (I): Milbemectin (J): Mepronil (K): Imidacloprid

  As is clear from the above test examples, the agricultural and horticultural composition according to the present invention has an excellent controlling effect due to a synergistic effect even at a concentration that shows only an insufficient controlling effect with individual antagonistic microorganisms and agricultural and horticultural agricultural chemical ingredients alone. Therefore, even if the amount of agricultural and horticultural agricultural chemical components used is reduced, a high control effect can be obtained. In addition, a wide antibacterial spectrum can be sufficiently expected.

Claims (15)

Bacillus sp. A plant disease control agent comprising one or more selected from spore of D747 (FERM BP-8234) and cupric hydroxide, mepronil, bench avalicarb isopropyl, penthiopyrad, and polyoxin complex . Bacillus sp. A plant disease control agent comprising one or more selected from spore of D747 (FERM BP-8234) and live BT spore, spinosad, milbemectin and imidacloprid . The agent according to claim 2, which also has an action of controlling harmful insects harmful to plants. Bacillus sp. A plant disease control agent comprising a spore of D747 (FERM BP-8234) and one or two selected from prohexadione calcium salt and trinexapacethyl . The agent according to claim 4, which also has at least one of the functions of regulating plant growth, grass shape, tree shape, flowering, and fruit setting. The agent according to any one of claims 1 to 5, wherein the plant disease is a plant disease caused by Botrytis and / or Pyricularia. The agent according to claim 6, wherein the plant disease is a plant disease caused by Botrytis cinerea and / or Pyricularia oryzae. Method for controlling plant diseases, which comprises treating the plants a composition according to any one of claims 1-7. Characterized by processing the plant bodies agent according to claim 3, method for controlling plant diseases and pests. A method for controlling at least one of plant disease control, plant growth, plant shape, tree shape, flowering, and fruit setting, characterized by treating the agent according to claim 5 with a plant body. Bacillus sp. Suspension of D747 (FERM BP-8234) and one or more selected from cupric hydroxide, mepronil, bench abaricarb isopropyl, penthiopyrad, polyoxin complex are suspended in water to form a mixed solution, A method for controlling plant diseases, which comprises treating the mixture with a plant. Bacillus sp. Suspends of D747 (FERM BP-8234) and live BT spores, spinosad, milbemectin and imidacloprid are suspended in water to form a mixed solution, and the mixed solution is processed into a plant body. A method for controlling plant diseases and pests. Bacillus sp. Suspending D747 (FERM BP-8234) and one or two selected from prohexadione calcium salt and trinexapac ethyl in water to form a mixed solution, and treating the mixed solution into a plant A method for controlling plant diseases and controlling at least one of plant growth, grass shape, tree shape, flowering, and fruit setting. The method according to any one of claims 8 to 13, wherein the plant disease is a plant disease caused by a genus Botrytis and / or a bacterium belonging to the genus Pyricularia. The method according to claim 14, wherein the plant disease is a plant disease caused by Botrytis cinerea and / or Pyricularia oryzae.