JP5034230B2 - Molded body and container - Google Patents

Description

  The present invention relates to a molded body in contact with a solution containing at least a calcium salt and a phosphate or carbonate, and particularly relates to a molded body that uses the molded body as a container or a tubular body.

  Unlike glass containers, containers and tubes formed from synthetic resin compositions are lightweight, hard to break, and easy to handle. Therefore, storage containers for blood, chemicals, infusions, and tubes such as tubes Widely used as a body.

  Recently, synthetic resin is used for what is called a culture vessel used when cells are cultured in a medium. For example, a medium is placed in a bag-like container made of a gas-permeable film. An encapsulated one has been proposed (see, for example, Patent Documents 1 and 2). In such a culture vessel containing a medium, after introducing and sealing the cells to be cultured in the culture vessel, it can be simply left in a culture vessel under a carbon dioxide atmosphere, for example, without being contaminated with bacteria. There is an advantage that cell culture can be performed.

  FIG. 5 is an example of a conventional culture vessel. As shown in the figure, the culture container 90 is configured by sealing a culture medium 92 in a gas-permeable container main body 91, and the container main body 91 is provided with three ports 93, 94, and 95. Ports 93 and 94 are used for sampling during culture and collection of cultured cells. A pipe body 98 made of polyvinyl chloride is connected to the port 95, and the distal end side of the pipe body 98 is connected to the pipe bodies 96 and 97 and branches in two directions. One distal end side tube 96 is used to inject the culture medium 92 into the container main body 91 when the culture container 90 is manufactured. After the manufacture, the distal end is fused by a heat seal, a high frequency seal or the like. Are sealed. The other distal end side tube body 97 is for injecting cells to be cultured into the container body 91, and the distal end is sealed with a cap or the like.

  When the culture vessel 90 is used, a syringe (not shown) filled with cells to be cultured is connected to the tip of the tube body 97, and the cells 97 and 98 are used as flow paths in the container body 91. Inject. Thereafter, the tube body 98 is sealed at a position C shown in the figure, and the container body 91 is sealed. For sealing the tubular body 98, a sealing means such as a pinch cock, a clip, heat sealing or fusion by high frequency sealing is used.

JP-A-6-78968 Japanese Patent Publication No. 6-97986

  In sealing the tubular body 98, the pinch cock or clip may be caught by an instrument or the like when moving the culture vessel 90 or performing other operations. On the other hand, in the case of fusion by heat sealing or high-frequency sealing, there is no risk of disengagement like a pinch cock or the like, and after fusion, the fusion part is cut so that the tip side is closer to the fusion part. The tubes 96 and 97 can be separated from the container body 91. Thereby, when carrying the culture vessel 90 or leaving it in the incubator, there is an advantage that unnecessary tubes 96 and 97 do not interfere with the work.

  However, the inventors have found that the fusion of the tube body 98 becomes incomplete after several days have passed since the culture vessel 90 was manufactured. Further, when a solution containing calcium salt and phosphate or carbonate is sealed in the culture vessel 90, white matter is deposited on the inner wall of the tube 98 to prevent the tube 98 from being fused, It was found that particulate matter precipitated in the container 90.

  The present invention has been made in view of such problems, and prevents the generation of precipitates that cause incomplete fusion in a molded body for contacting a solution containing at least a calcium salt and a phosphate or carbonate. It aims at providing the means to do.

  Another object of the present invention is to ensure that the tube connected to the container body in which a solution containing at least a calcium salt and a phosphate or carbonate is enclosed is at an arbitrary position where the solution can flow. The object is to provide means capable of fusing.

  The present inventors can seal a tube body connected to a container body in which a solution containing at least calcium salt and phosphate or carbonate is sealed by fusion at an arbitrary position immediately after manufacture. As a result of finding the problem that the fusion of the tubular body becomes incomplete as the number of days after manufacture elapses, and as a result of earnest research to solve the problem, the substance deposited on the inner surface of the tubular body is melted. The present inventors have found a fact that has a negative effect on wearing, and have focused on this fact to complete the present invention.

  This deposited substance is considered to be generated by a chemical reaction between a molded body formed by molding the synthetic resin composition and a solution containing at least a calcium salt and a phosphate or carbonate. In general, stearates are added to the synthetic resin composition as a heat stabilizer. The heat stabilizer is added to prevent thermal deterioration of the resin component when molding the synthetic resin composition, and prevents coloring of the molded body due to resin burns. This stearic acid salt, which is a heat stabilizer, was found to precipitate as a substance that inhibits fusion by reacting with the substance in the solution, and the present invention was completed.

The present invention provides a container body in which a solution containing at least a calcium salt and phosphate or carbonate is enclosed, and the container body so that the solution can flow into a pipe through an opening provided in the container body. And a tube body that can be sealed by fusion at an arbitrary position where the solution can flow, the tube body being 0 parts per 100 parts by weight of the thermoplastic resin component . It is molded from a synthetic resin composition containing 0.0001 to 0.01 parts by weight of calcium stearate.

  In the present invention, the molded body is formed by molding a synthetic resin composition, and a known molding method such as injection molding, extrusion molding, or casting molding can be arbitrarily employed. Arbitrary shapes, such as a sheet shape, a tube shape, a bag shape, a bottle shape, can be employ | adopted for the shape of a molded object. In addition, by forming a sheet-shaped molded body into a cylindrical shape and fusing the edges, or by stacking two sheet-shaped molded bodies and fusing the edges, a bag shape can be formed. A tubular tube body may be connected to the container.

The thermoplastic resin component is preferably a vinyl chloride resin.

According to the container according to the present invention, the tube connected to the container main body, in which a solution containing at least calcium salt and phosphoric acid or carbonate is sealed, is connected to the thermoplastic resin component of 100 parts by weight. Therefore, even if it is in the same atmosphere as the solution sealed in the container body or the solution flows, it was formed from a synthetic resin composition containing 0.0001 to 0.01 parts by weight of calcium stearate. Even if contact is made, precipitation of calcium stearate or the like can be prevented from being deposited on the inner surface of the tube. Thereby, the said pipe body can be melt | fused and sealed at the arbitrary positions where the said solution can flow.

  Hereinafter, preferred embodiments of the present invention will be described with reference to the drawings as appropriate. In the present embodiment, the culture container is described as an example of the molded body according to the present invention. However, the molded body according to the present invention is not limited to the culture container, and stores blood, drug solution, infusion, and the like. It can be widely used for containers for distribution and pipes for distribution.

  FIG. 1 shows a container 1 according to an embodiment of the present invention. As shown in the figure, the container 1 is formed by connecting a tube body 3 to a container body 2 made of a polyolefin resin having gas permeability.

  As shown in the figure, the container body 2 is formed by superposing two substantially identical rectangular resin sheets and sealing the periphery thereof by a fusion means such as a heat seal or a high frequency seal. As a constituent material of the resin sheet, a gas such as oxygen or carbon dioxide is permeable, and specifically, a polyolefin-based resin sheet such as polyethylene, polyvinyl chloride, or polyethylene-vinyl acetate copolymer is used. Can be used. By constructing the container body 2 from such a gas-permeable resin sheet, for example, if the sealed container 1 is left in an incubator under a carbon dioxide atmosphere, cells are cultured in the container body 2. At this time, oxygen, carbon dioxide, and the like that are necessary for the gas flow through the resin sheet, and there is an advantage that cells can be cultured in the container body 2 without being contaminated with bacteria.

  The container body 2 is provided with three ports 4, 5, 6 (openings) on one side thereof. These ports 4, 5, and 6 sandwich the tubular member 3 and the cylindrical member made of synthetic resin between the resin sheets when the two resin sheets constituting the container body 2 are fused. It is formed by. The cylindrical members constituting the ports 4 and 5 are so-called membrane tube bodies 7 in which one end of the tube body outside the container body 2 is sealed with a diaphragm. The ports 4 and 5 formed by the membrane tube body 7 are used when taking out the cultured cells from the container body 2 or sampling the cells during the culture. In addition, the ports 4 and 5 are arbitrary structures in this invention, and the number of these ports is not specifically limited. Of course, the configuration of each port can be changed to a known and arbitrary configuration.

  The port 6 is fused so that the tube body 3 is sandwiched between two resin sheets constituting the container body 2. The tube body 3 extends from the container body 2 and is connected to the branch connector 8. The branch connector 8 is a cylindrical member made of synthetic resin, has three openings communicated with each other, and a tubular body can be connected to each opening. Tubes 9 and 10 are connected to the other openings of the branch connector 8, so that the tube 3 extending from the back body 2 is branched into two tubes 9 and 10. Yes.

  The tube body 9 is used for injecting a culture medium into the container body 2. In the container 1 according to the present embodiment, since the culture medium is sealed in the container main body 2 at the time of manufacture, the tube body 9 is used at the time of manufacturing the container 1, and the end of the tube 9 is heated after the manufacture of the culture medium. It is fused and sealed with a seal or a high frequency seal.

  The tube body 10 is used to inject cells to be cultured into the container body 2. The end of the tube body 10 is sealed with a cap 11, and in use, the cap 11 is removed, and a syringe filled with a cell suspension is connected to the tube body 10, and the tube body 3, A cell suspension is injected into the back body 2 using 10 as a flow path. In addition, the sealing means of the tubular body 10 is not limited to the cap 11, and other known and arbitrary sealing means can be used.

  If the medium enclosed in the container body 2 is a solution containing at least a calcium salt and a phosphate or carbonate, other components may be appropriately selected according to the cells to be cultured. For example, as long as it is a medium for animal cells, BME medium, MEM medium, RPMI-1640 medium, and the like can be used. Further, when the container body 2 is used as a container for infusion, sodium chloride or glucose may be added to the solution to be sealed. The cells cultured in the container 1 are not limited to animal cells, but are particularly included in cells contained in living body fluids such as blood, spinal fluid, and lymphocytes, living tissues, organs, and the like. Suitable for cell culture.

  Calcium salts are used as intracellular messengers for regulating various functions such as cell skeleton maintenance and intracellular transmission. Carbonate is used for the purpose of maintaining the above-mentioned medium at a predetermined pH and for the purpose of adding it as a carbon source for cells to be cultured. For example, 2- [4- (Hydroxyethyl) -1-piperazinyl] ethanesulfonic aid reagent (hereinafter referred to as “HEPES”) is a HEPES-balanced Krebs-Ringer bicarbonate by adjusting the pH even as a solution of HEPES alone. It can also be used as a buffer (hereinafter abbreviated as “HEPES buffer”), but when used as a HEPES buffer, sodium chloride, potassium chloride, calcium chloride, magnesium chloride, sodium dihydrogen phosphate, Sodium bicarbonate is added along with HEPES. Phosphate is used for the purpose of maintaining a solution such as a medium at a predetermined pH, the source of cell membranes, and ATP synthesis. In the present invention, the action of calcium salt or carbonate is not particularly limited. For example, when the container according to the present invention is used as a container for infusion storage instead of a culture container, the calcium salt is added to the solution as a calcium supply source.

  If the added phosphate is, for example, disodium hydrogen phosphate, the final concentration is preferably 20 to 1600 mg / L, and more preferably 40 to 800 mg / L. For example, if the calcium salt to be added is calcium chloride, the final concentration is preferably 30 to 2000 mg / L, and more preferably 100 to 300 mg / L. For example, if the carbonate to be added is sodium hydrogen carbonate, the final concentration is preferably 200 to 9000 mg / L, and more preferably 1200 to 40000 mg / L.

  The medium enclosed in the container 1 may be any medium containing calcium salt and phosphate or carbonate, and particularly in a container containing calcium chloride and disodium hydrogen phosphate or sodium hydrogen carbonate. Precipitation of substances that cause incomplete fusion by reaction with stearate is significant.

  Since the purpose of using carbonate is not directly related to the gist of the present invention, the solution sealed in the container body 2 may contain carbonate used for other purposes. In addition to sodium hydrogen carbonate, the carbonate may be other carbonates such as sodium carbonate and magnesium carbonate hydroxide.

  The container 2 and the tube 3 are made of synthetic resin such as thermoplastic elastomer such as soft polyvinyl chloride, polyethylene, polypropylene, polyester, ethylene-vinyl acetate copolymer, polyurethane, polyester elastomer, and styrene-butadiene-styrene copolymer. In view of flexibility, softness, and cost, a tube made of soft polyvinyl chloride is suitable, but is not particularly limited to polyvinyl chloride.

  The container 2 and the tube 3 made of soft polyvinyl chloride are obtained by molding a resin composition in which a plasticizer is blended with a vinyl chloride resin. Examples of the vinyl chloride resin include polyvinyl chloride, vinyl chloride monomer copolymer, vinyl chloride monomer graft polymer, and chlorinated vinyl chloride. In such a vinyl chloride resin molding process, an organic tin compound and a stearic acid compound are added as stabilizers to the vinyl chloride resin component in order to prevent deterioration of the mechanical strength and color tone of the molded product. . Thereby, the thermal stability at the time of shaping | molding of a vinyl chloride-type resin composition improves.

  The calcium stearate is blended at 0.01 parts by weight or less with respect to 100 parts by weight of the vinyl chloride resin. Generally, in order to improve the thermal stability at the time of molding of a vinyl chloride resin composition, calcium stearate is blended at 0.3 to 10 parts by weight with respect to 100 parts by weight of the vinyl chloride resin. However, when the container 1 is connected to the container body 2 as the tubular body 3 and is used in the same atmosphere as the medium enclosed in the container body 2 or in a state where the medium can be contacted, Calcium stearate is deposited on the inner surface of the tube body 3 due to the influence of at least calcium salt and phosphate or carbonate in the medium. The precipitated calcium stearate inhibits the fusion of the tube 3 and the tube 3 is not completely sealed.

  Therefore, the amount of calcium stearate deposited on the inner surface of the tube 3 is reduced by setting the calcium stearate contained in the tube 3 to 0.1 parts by weight or less with respect to 100 parts by weight of the vinyl chloride resin. be able to. Thereby, the pipe body 3 can be reliably fused. The fused portion of the tube body 3 is cut to hold the container body 2 in a closed state from the outside, and the distal end side of the tube body 3, the branch connector 8, and the tube bodies 9 and 10 are separated from the back body 2.

  Further, if calcium stearate is not blended at all with respect to 100 parts by weight of the vinyl chloride resin, the mechanical strength of the molded tube 3 may be lowered or the tube 3 may be colored. It is preferable to blend calcium phosphate in the range of 0.001 to 0.1 parts by weight with respect to 100 parts by weight of the vinyl chloride resin. In addition, you may mix | blend another lubricant, a processing aid, antioxidant, etc. with the vinyl chloride-type resin composition used as the material of the pipe body 3 as needed.

  As described above, the container 1 according to the present embodiment includes a tube body 3 connected to the container body 2 in which a medium containing at least a calcium salt and a phosphate or carbonate is enclosed via the port 6. Since it was formed from a vinyl chloride resin composition containing 0.01 parts by weight or less of calcium stearate with respect to parts by weight of the vinyl chloride resin, it was in the same atmosphere as the medium sealed in the container body 2. However, even if the culture medium flows and comes into contact, precipitation of calcium stearate or the like from the inner surface of the tube 3 can be reduced.

  Therefore, after injecting the cell suspension into the container body 2 using the tubes 3 and 10 as flow paths, the tube 3 can be fused at a position P by heat sealing or high frequency sealing and completely sealed. It is. After the fusion, as shown in FIG. 2, the tube body 3 is cut at the fusion portion 12 of the tube body 3, so that the distal end side of the tube body 3, the branch connector 8, and the tube bodies 9 and 10 are connected to the container body. 2 can be separated. As a result, when the container 1 is carried or left in the incubator, the unnecessary tubes 9 and 10 do not interfere with the work, so the usability of the container body 2 is improved and the space in the incubator is reduced. Can be realized.

Hereinafter, modifications of the present invention will be described.
FIG. 3 shows a container 13 according to a modification of the present invention. As shown in the figure, the container 13 is formed by connecting two container bodies 2 and a secondary container 14. The container main body 2 is the same as that of the said embodiment, and the same code | symbol has shown the same member in the figure. As described above, since the container body 2 is made of a polyolefin resin having gas permeability, gas components in the atmosphere circulate in the container body 2 during storage and transportation, and are enclosed in the container body 2. May affect the pH of the prepared medium. Therefore, the container 1 is sealed in an airtight bag or the like during storage or transportation after manufacture, and is taken out from the bag during use. On the other hand, in this container 13, an airtight secondary container 14 is connected to the container main body 2 via pipe bodies 15 and 18, and the medium is enclosed in the secondary container 14. Thereby, the gas component in air | atmosphere does not distribute | circulate in the secondary container 14 at the time of storage or transportation, and a culture medium can be maintained at desired pH until it is used.

  The secondary container 14 has the same configuration as the container body 2 except that a confidential resin sheet is used as a constituent material, and two substantially identical rectangular resin sheets are superposed and their peripheral edges are overlapped. Is sealed by a fusing means such as a heat seal or a high frequency seal.

  The secondary container 14 is provided with one port 16 (opening) on one side thereof. Similarly to the port 6, the port 16 is also attached by sandwiching the tube body 15 between two resin sheets.

  The tube body 15 extends from the secondary container 14 and is connected to the connector 17. The connector 17 is a cylindrical member made of a synthetic resin, and is a so-called broken bar having a fragile fracture portion inside. The rupture portion closes the flow path in the connector 17, and the connector 17 is opened by holding the finger 17 from the outside with a finger or the like, and the rupture portion is ruptured so that the flow path is opened while maintaining the closing property with respect to the outside. It is supposed to be. A tube body 18 is connected to the other end of the connector 17, and the tube body 18 forms a port 19 of the container body 2.

  The medium enclosed in the secondary container 14 is a medium similar to that in the above embodiment, and is a solution containing at least carbonate. In addition, the tube 15 is made of a heat-resistant material such as soft polyvinyl chloride, polyethylene, polypropylene, polyester, ethylene-vinyl acetate copolymer, polyurethane, polyester elastomer, styrene-butadiene-styrene copolymer, and the like. It is a tube made of a synthetic resin such as a plastic elastomer, and is preferably made of soft polyvinyl chloride as a main material in consideration of flexibility, flexibility and cost.

  The tube 15 made of soft polyvinyl chloride is obtained by molding a resin composition in which a plasticizer is blended with a vinyl chloride resin. Examples of the vinyl chloride resin include polyvinyl chloride, vinyl chloride monomer copolymer, vinyl chloride monomer graft polymer, and chlorinated vinyl chloride. The tubular body 15 is obtained by molding from a synthetic resin composition containing 0.1 parts by weight or less of calcium stearate with respect to 100 parts by weight of the vinyl chloride resin. Further, in order to prevent the mechanical strength of the molded product from being lowered or colored, it is preferable to blend calcium stearate in the range of 0.001 to 0.1 parts by weight with respect to 100 parts by weight of the vinyl chloride resin. is there. In addition, you may mix | blend another lubricant, a processing aid, antioxidant, etc. with the vinyl chloride-type resin composition used as the material of the pipe body 15 as needed.

  Thereby, the amount of precipitates such as calcium stearate deposited on the inner surface of the tube body 15 can be reduced, and the tube body 15 can be reliably fused. That is, the container 13 according to the present modification includes 100 parts by weight of the tube body 15 connected via the port 16 to the secondary container 14 in which a medium containing at least calcium salt and phosphate or carbonate is enclosed. In the same atmosphere as the medium sealed in the secondary container 14, the vinyl chloride resin was molded from a vinyl chloride resin composition containing 0.1 parts by weight or less of calcium stearate. Alternatively, even if the culture medium flows and contacts, the amount of precipitates such as calcium stearate from the inner surface of the tube body 15 can be reduced.

  Therefore, the broken portion of the connector 17 is broken to open the flow path, and after the medium is injected from the secondary container 14 into the container body 2 using the tubular bodies 15 and 18 as the flow path, the tubular body 15 is heated at the position Q. It is possible to seal completely by fusing with a seal or a high frequency seal. After the fusion, as shown in FIG. 4, the tube body 15 is cut at the fusion portion 20 of the tube body 15, whereby the port 15 side part of the tube body 15 and the secondary container 14 are removed from the container body 2. Can be separated. As a result, the empty secondary container 14 does not interfere with the operation of injecting the cell suspension into the container body 2, so that the usability of the container body 2 is improved and the space in the incubator is saved. realizable.

Examples of the present invention will be described below.
In the main body container 2 made of a polyethylene sheet, 0 parts by weight of calcium stearate (Example 1), 0.002 parts by weight (Example 2), 0.1 parts by weight (comparative) with respect to 100 parts by weight of the vinyl chloride resin. Examples 1), 0.3 parts by weight (Comparative Example 2) were respectively connected to each tubular body 3 obtained by molding a vinyl chloride resin composition, and Examples 1, 2 and Comparative The container 1 of Examples 1 and 2 was obtained.

  In the main body container 2 of each example and each comparative example, 140 mg / L sodium chloride, 48 mg / L sodium dihydrogen phosphate, 5958 mg / L HEPES balanced Krebs-Ringer bicarbonate buffer, 350 mg / L The culture medium which mixed sodium hydrogencarbonate of was enclosed.

After the container 1 of each example and each comparative example was allowed to stand at room temperature for 1 week, the deposit on the inner surface of the tube 3 was visually confirmed and evaluated according to the following evaluation criteria.
When no precipitate is observed: “None”
When no precipitate is observed, but slightly observed when sputtered with a spatula: “trace”
When precipitates are clearly observed: “Large”
Furthermore, about the thing by which the deposit was confirmed visually, a deposit was analyzed with a Fourier-transform infrared spectrophotometer (henceforth "FT-IR"), and a calcium stearate (in the table | surface, "st-Ca"). ") Is abbreviated as follows.
When calcium stearate is detected: “st-Ca detection”
When calcium stearate is not detected: “st-Ca not detected”
Not analyzed: “Unanalyzed”
Further, the tube body 3 was fused by a method described in the instruction manual with a high frequency seal (Zebra Co., Ltd., Omnisealer model 2380), and evaluated according to the following evaluation criteria.
If the fused part cannot be released by hand: “○”
When the fused part can be released by hand: “△”
If fusion is not possible: “×”
These evaluation results are shown in Table 1.

なお、析出物をＦＴ−ＩＲで確認したところ、比較例１及び比較例２で析出された析出物は主にステアリン酸カルシウムであった。

In addition, when the deposit was confirmed by FT-IR, the deposit deposited in Comparative Example 1 and Comparative Example 2 was mainly calcium stearate.

  As is clear from Table 1, in Comparative Examples 1 and 2, a large amount of precipitates containing calcium stearate as a main component was deposited on the inner surface of the tube 3, and the fusion by the high frequency seal was insufficient or could not. On the other hand, in Examples 1 and 2, deposits did not deposit on the inner surface of the tube body 3, and fusion by high frequency sealing was good. In Example 1, the tube 3 was confirmed to be brown.

  A vinyl chloride resin composition containing 0.01 parts by weight of calcium stearate (Examples 4 to 14) and 0.3 parts by weight (Comparative Examples 3 to 13) with respect to 100 parts by weight of the vinyl chloride resin is molded. Thus, a tube 3 was obtained. Each tubular body 3 was connected to a main body container 2 made of a polyethylene sheet to form each container 1 of Examples 4 to 14 and Comparative Examples 3 to 13.

  In the main body container 2 of Example 4 and Comparative Example 3, 48 mg / L disodium hydrogen phosphate, 5958 mg / L HEPES buffer, 140 mg / L calcium chloride, and 350 mg / L sodium bicarbonate were mixed. The resulting solution was enclosed.

  In the main body container 2 of Example 5 and Comparative Example 4, a solution obtained by mixing 48 mg / L disodium hydrogen phosphate, 5958 mg / L HEPES buffer, and 140 mg / L calcium chloride was sealed.

  In the main body container 2 of Example 6 and Comparative Example 5, 48 mg / L disodium hydrogen phosphate, 5958 mg / L HEPES buffer, 350 mg / L calcium chloride, and 350 mg / L sodium bicarbonate were mixed. The resulting solution was enclosed.

  In the main body container 2 of Example 7 and Comparative Example 6, a solution obtained by mixing 48 mg / L disodium hydrogen phosphate, 140 mg / L calcium chloride, and 350 mg / L sodium bicarbonate was sealed.

  In the main body container 2 of Example 8 and Comparative Example 7, a solution obtained by mixing 5958 mg / L HEPES buffer, 140 mg / L calcium chloride, and 350 mg / L sodium hydrogen carbonate was sealed.

  In the main body container 2 of Example 9 and Comparative Example 8, a solution obtained by mixing 48 mg / L disodium hydrogen phosphate and 5958 mg / L HEPES buffer was sealed.

  In the main body container 2 of Example 10 and Comparative Example 9, a solution obtained by mixing 48 mg / L disodium hydrogen phosphate and 140 mg / L calcium chloride was sealed.

  In the main body container 2 of Example 11 and Comparative Example 10, a solution formed by mixing 48 mg / L disodium hydrogen phosphate 350 mg / L sodium hydrogen carbonate was sealed.

  In the main body container 2 of Example 12 and Comparative Example 11, a solution obtained by mixing 5958 mg / L of HEPES buffer and 140 mg / L of calcium chloride was sealed.

  In the main body container 2 of Example 13 and Comparative Example 12, a solution obtained by mixing 5958 mg / L HEPES buffer and 350 mg / L sodium hydrogen carbonate was sealed.

  In the main body container 2 of Example 14 and Comparative Example 13, a solution obtained by mixing 140 mg / L calcium chloride and 350 mg / L sodium hydrogen carbonate was sealed.

  After leaving the container 1 of each example and each comparative example at 4 ° C. for 1 week, visually confirming the precipitation of calcium stearate as a main component on the inner surface of the tube 3 as described above, The tube 3 was fused with a high frequency seal, and the state was evaluated in the same manner as described above. Furthermore, about what the deposit was confirmed visually, it analyzed by FT-IR and evaluated similarly to the above-mentioned. These results are shown in Table 2.

  As is clear from Table 2, in Examples 4 to 14, no precipitate was observed in the tube body 3, and all showed good fusion results. In Comparative Examples 3, 4, 6, 7, 9, and 13, precipitates were observed in the tube 3 and fusion could not be performed. And as a result of analyzing by FT-IR, it was mainly confirmed that it was calcium stearate. Thereby, it became clear that the precipitation of the precipitate mainly composed of calcium stearate involves at least calcium salt and phosphate or carbonate in the solution. It was also found that the tube 3 could be fused if the calcium stearate contained in the tube 3 was 0.01 parts by weight with respect to 100 parts by weight of the resin component.

  A propylene-based resin composition obtained by blending 0.0002 parts by weight of calcium stearate with 100 parts by weight of polypropylene-based resin was molded, and the obtained tubular body and container were used as Container 1 of Example 15. In the container 1, a medium comprising 140 mg / L sodium chloride, 48 mg / L sodium dihydrogen phosphate, 5958 mg / L HEPES buffer, and 350 mg / L sodium bicarbonate was sealed. After this container 1 was allowed to stand at 4 ° C. for 1 week, the precipitation of calcium stearate on the inner surface of the tube 3 was visually confirmed, and no precipitate was observed even when sputtered with a spatula.

FIG. 1 is a diagram showing a schematic configuration of a container 1 according to an embodiment of the present invention. FIG. 2 is a view showing a state in which the tubular body 3 is cut at the fused portion 12. FIG. 3 is a diagram showing a schematic configuration of the container 13 according to a modification of the present invention. FIG. 4 is a view showing a state in which the tubular body 15 is cut at the fused portion 20. FIG. 5 is a diagram showing a schematic configuration of a conventional culture vessel 90.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1,13 ... Container 2 ... Container main body 3,15 ... Tube 4, 5, 6, 16, 19 ... Port (opening)

Claims (2)

A container main body in which a solution containing at least calcium salt and phosphate or carbonate is enclosed; and the solution is connected to the container main body through an opening provided in the container main body so that the solution can flow into the pipe; A tube body that can be sealed by fusion at any position where the solution can flow,
The said tubular body is a container shape | molded from the synthetic resin composition containing 0.0001-0.01 weight part calcium stearate with respect to 100 weight part thermoplastic resin component . The container according to claim 1, wherein the thermoplastic resin component is a vinyl chloride resin.

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