JP4980160B2 - Mushroom cultivation method - Google Patents

Description

  The present invention relates to a novel method for cultivating mushrooms.

  The consumption of mushrooms, combined with food and health foods, is very large at approximately 275.0 billion yen in Japan alone (according to the 2005 Forestry Agency survey). Reflecting this, demand is increasing. Many of these mushrooms are artificially cultivated. For example, a hole is made in the raw wood and inoculated with the inoculum, and the fungus is spread in a natural environment such as a forest for one year (raw wood cultivation) It is cultivated by inoculating seeds that have been hardened by adding a nutrient source such as cultivated (fungus bed cultivation) in a facility equipped with air conditioning equipment. On the other hand, depending on the type of mushroom, artificial cultivation itself is difficult, and even if artificial cultivation is successful, commercialization may be difficult due to slow growth like Hatake-shimeji.

  As a method for promoting the growth of mushrooms, it is generally performed to appropriately add an application agent containing a nitrogen source such as bran and rice bran, and such fertilization has a certain effect.

  However, the effect of fertilization is naturally limited, and even if the amount of fertilizer used is large, not only a certain level of mushroom growth promotion effect can be expected, but if too much fertilizer is applied, it will hinder plant growth. Discarding an over-nutrient medium can eventually contaminate the soil.

  Thus, there is a need in the art to reduce fertilizer usage and increase mushroom production without degrading the soil environment.

  Ketol fatty acid isolated from Lemna paucicostata by the present inventor not only acts on the passive life activity of plants that occur when plant growth is suppressed, such as the promotion of flower bud formation. No. 11-29410), plant activation, for example, promotion of plant growth (expansion of foliage, promotion of growth of tuber tuberous root, etc.), inhibition of dormancy, imparting resistance to plant stress, regulation of plant growth such as anti-aging It also has an action (Japanese Patent Laid-Open No. 2001-131006).

  In Japanese Patent Application Laid-Open No. 2001-131006, ketol fatty acid is also referred to as a growth promoter for mushrooms. Specifically, the hyphal growth effect of Tamogitake and the fruit body growth promotion effect of Shiitake mushroom were confirmed in Test Example D. Yes. On the other hand, the gazette makes no mention of the optimal method of using ketol fatty acid for mushrooms and the time of use.

JP-A-11-29410 JP 2001-131006 A

  An object of this invention is to provide the novel cultivation method of mushrooms.

  In order to promote the growth of mushrooms, the present inventor examined the optimal method of using a specific ketol fatty acid. As a result, the mycelium after stress exposure, in particular, the mycelium after the fungus scraping process in fungal bed cultivation, It became clear that the amount of mushroom production increased by applying.

That is, this application includes the following inventions.
[1] Stress of a ketol fatty acid having 4 to 24 carbon atoms in which the carbon atom constituting the carbonyl group and the carbon atom in which the hydroxyl group is bonded is in the α-position, and the ketol fatty acid having 4 to 24 carbon atoms A method for cultivating mushrooms comprising a step of applying to a mycelium after exposure.
[2] The method for cultivating mushrooms according to [1], which is exposed to stress by a fungus scraping step in fungus bed cultivation.
[3] The method for cultivating mushrooms according to [1] or [2], wherein the application step is performed between the end of the fungus scraping step and the formation of the primordium.
[4] The method for cultivating mushrooms according to any one of [1] to [3], wherein the application step is performed within 24 hours after the end of the fungus scraping step.

  By applying a specific ketol fatty acid to mushrooms during the fungus scraping process in fungus bed cultivation, the yield of the final fruiting body is significantly increased as compared with the case where it is applied before the process. In addition, ketol fatty acid in an amount of about 3 to 60 ppm is required for promoting and activating flower bud formation in general plants. In the present invention, ketol fatty acid exhibits its effect from a concentration of about 1/100.

  Here, ketol fatty acid has a property that it is often produced when a plant is stressed. While not intending to be bound by theory, it is believed that the fungal scraping process causes stress to the mycelium, and the mushroom growth promoting effect according to the present invention is sprayed with ketol fatty acids generated by such stress. It seems to be caused by the action of fatty acids on the mycelium.

  Other effects include shortening the cultivation period. For example, when applied to eringi, a cultivation period of 9 days is usually required, but by using the cultivation method of the present invention, it is possible to accelerate the 1-2 day harvest date. In particular, the cultivation period can be further shortened by carrying out the method during the mycelium culture period. The mycelium culture is a step of growing mycelium in a mycelium (medium) before forming fruit bodies.

  As described above, the ketol fatty acid used in the present invention is a ketol fatty acid having 4 to 24 carbon atoms (hereinafter, this ketol fatty acid is also referred to as “specific ketol fatty acid”). That is, the specific ketol fatty acid is a fatty acid having 4 to 24 carbon atoms and having a hydroxyl group of alcohol and a carbonyl group of ketone in the same molecule.

  In the present invention, the specific ketol fatty acid has a carbon atom in which the carbon atom constituting the carbonyl group and the hydroxyl group are bonded at the α-position or the γ-position, in order to exert a desired mushroom growth effect. The α-position is particularly preferable from this viewpoint.

  The specific ketol fatty acid has a desired mushroom having 1 to 6 carbon-carbon double bonds (however, the number of double bonds does not exceed the number of carbon bonds of the ketol fatty acid). It is preferable for exhibiting the proliferation effect of the species.

  The specific ketol fatty acid preferably has 18 carbon atoms and two carbon-carbon double bonds are present.

  Specific examples of the specific ketol fatty acid include, for example, 9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid (hereinafter sometimes referred to as the specific ketol fatty acid (I)), 13-hydroxy -12-oxo-9 (Z), 15 (Z) -octadecadienoic acid (hereinafter sometimes referred to as specific ketol fatty acid (II)), 13-hydroxy-10-oxo-11 (E), 15 (Z ) -Octadecadienoic acid (hereinafter sometimes referred to as specific ketol fatty acid (III)), 9-hydroxy-12-oxo-10 (E), 15 (Z) -octadecadienoic acid [hereinafter referred to as specific ketol fatty acid ( IV) ”and the like.

  The chemical structural formulas of the specific ketol fatty acids (I) to (IV) are described below.

  The chemical structural formulas of other specific ketol fatty acids and the chemical synthesis methods of these specific ketol fatty acids are as disclosed in JP-A-2001-131006 (listed above).

  According to the present invention, the yield of mushrooms can be increased by applying the specific ketol fatty acid to the mycelium after stress exposure.

  When cultivating mushrooms by fungal bed cultivation, the sterilized mushroom medium is packed in a cultivation bottle such as a pot, and then the seed fungus is inoculated on the mushroom medium and the mycelium is cultured. After a predetermined culture period, scraping old bacteria on the surface of the medium (“fungal scraping” step), the mycelium is textured and becomes a fruit body (primitive formation).

  Here, the fungus scraping applies physical stress to the mycelium as described above. In a specific aspect, the present invention provides a method for cultivating mushrooms, which comprises applying the specific ketol fatty acid to the mycelium after the fungus scraping step in fungus bed cultivation.

  Preferably, the application is performed between the end of the fungus scraping process and the formation of the primordium. The application of the specific ketol fatty acid to the mycelium is preferably performed within 24 hours from the end of the fungus scraping step, and more preferably within 6 hours from the viewpoint of increasing the yield.

  The stress exposure used in the present invention is not intended to be limited to the fungus scraping step, and any stress may be used as long as it gives some stress to the mushrooms in the growth process. For example, dry stress, heat stress, osmotic pressure stress and the like can be mentioned.

  The upper limit of the amount of the specific ketol fatty acid applied to the mycelium of the mushroom is not particularly limited. On the other hand, the lower limit of the amount of the specific ketol fatty acid applied to the mycelium of the mushroom varies depending on the type of the mushroom and the size of the mycelium, but about 0.1 μM or more per application is a standard.

  The amount of the specific ketol fatty acid used can be selected according to the use mode, the type of mushroom to be used, and the specific dosage form containing the specific ketol fatty acid. As an aspect of the present invention, the specific ketol fatty acid can be used as it is. However, in consideration of the above-mentioned guideline for application of the specific ketol fatty acid, about 0.03 to 60 ppm is generally preferable with respect to the whole agent. Preferably, it is about 0.3 to 30 ppm. For example, in the case of oyster mushrooms and eringi, it is 0.05 to 50 ppm, preferably 0.1 to 40 ppm, and more preferably 0.3 to 30 ppm.

  Such dosage forms include, for example, dosage forms such as liquids, solids, powders, emulsions, bottom floor additives, etc., and depending on the dosage form, known carrier components that can be applied pharmacologically, Formulation adjuvants and the like can be appropriately blended so long as the growth promoting action of mushrooms, which is the desired effect of the present invention, is not impaired. For example, as a carrier component, when the dosage form containing a specific ketol fatty acid is a bottom floor additive or a solid agent, talc, clay, vermiculite, diatomaceous earth, kaolin, calcium carbonate, calcium hydroxide, clay, silica gel, etc. In the case of a liquid agent, water, aromatic hydrocarbons such as xylene, alcohols such as ethanol and ethylene glycol, ketones such as acetone, dioxane, tetrahydrofuran A liquid carrier such as ethers such as dimethylformamide, dimethyl sulfoxide, and acetonitrile is used as the carrier component. Examples of the adjuvant for preparation include anionic surfactants such as alkyl sulfates, alkylsulfonates, alkylarylsulfonates, dialkylsulfosuccinates, and cationic surfactants such as salts of higher aliphatic amines. , Polyoxyethylene glycol alkyl ether, polyoxyethylene glycol acyl ester, polyoxyethylene glycol polyhydric alcohol acyl ester, nonionic surfactant such as cellulose derivative, thickener such as gelatin, casein, gum arabic, A binder or the like can be appropriately blended.

  Further, if necessary, a general plant growth regulator, benzoic acid, nicotinic acid, nicotinic acid amide, pipecolic acid, etc. contain a specific ketol fatty acid as long as the desired effect of the present invention is not impaired. It can also be blended in the dosage form.

  The specific ketol fatty acid used in the above method may be sprayed, dripped, applied, etc. as a liquid or emulsion on a part or the whole of the mycelium and the mycelium in the fruit body primordium formation stage after the fungus scraping process, It can be absorbed from the medium as a powder.

  The frequency of application of the specific ketol fatty acid to mushrooms varies depending on the type of mushrooms, the purpose of application, etc., but basically the desired effect can be obtained by only one application. When applying multiple times, it is efficient to leave an interval of about 5 days.

  The kind of mushroom to which the specific ketol fatty acid can be applied is not particularly limited as long as it is cultivated by a cultivation method that requires a step of applying stress to the mushroom during cultivation, such as fungus scraping in fungus bed cultivation. Examples of such mushrooms include shiitake mushrooms, sea cucumbers, enokitake mushrooms, oyster mushrooms, beech shimeji mushrooms, maitake mushrooms, eringi mushrooms, mushroom shimeji mushrooms, ushiratake mushrooms, etc.

  Examples of the adjuvant for preparation include anionic surfactants such as alkyl sulfates, alkyl sulfonates, alkyl aryl sulfonates, dialkyl sulfone succinates, and cationic interfaces such as salts of higher aliphatic amines. Activators, polyoxyethylene glycol alkyl ethers, polyoxyethylene glycol acyl esters, polyoxyethylene glycol polyhydric alcohol acyl esters, nonionic surfactants of cellulose derivatives, thickeners such as gelatin, casein, gum arabic, and extenders Or a binder etc. can be mix | blended suitably.

  Furthermore, if necessary, a plant growth regulator, benzoic acid, nicotinic acid, nicotinic acid amide, pipecolic acid, etc. are blended in a dosage form containing a specific ketol fatty acid to the extent that the intended effect of the present invention is not impaired. I can do it.

  In the cultivation of mushrooms described later, 9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid is used as the specific ketolic acid. The specific ketolic acid was prepared using an enzymatic method as follows.

1. Preparation of rice germ-derived lipoxygenase 350 g of rice germ washed with petroleum ether, degreased and dried (250 g) was suspended in 1.25 L of 0.1 M acetate buffer (pH 4.5), and this suspension was homogenized. did.

  Subsequently, this homogenized extract was centrifuged at 16000 rpm for 15 minutes to obtain a supernatant (0.8 L). To the obtained supernatant, 140.8 g (0 to 30% saturation) of ammonium sulfate was added and left overnight at 4 ° C. Thereafter, centrifugation was performed at 9500 rpm for 30 minutes, and 232 g (70% saturation) of ammonium sulfate was added to the obtained supernatant (0.85 L), and the mixture was allowed to stand at 4 ° C. for 5 hours.

  Next, the mixture is centrifuged at 9500 rpm for 30 minutes in the same manner, and the resulting precipitate (30 to 70% saturated fraction of rice germ extract) is dissolved in 300 ml of pH 4.5 acetate buffer solution at 63 ° C. For 5 minutes. Thereafter, the generated precipitate was removed, and the resulting supernatant was desalted by dialysis (3 L × 3) using an RC dialysis tube (Spectrum pore 4: MWCO 12000-14000) and then the desired rice germ. A crude enzyme solution of lipoxygenase derived therefrom was obtained.

2. Preparation of allene oxide synthase derived from flax seed Flax seed was purchased from Ichimaru Falcos. To 200 g of this flaxseed, 250 ml of acetone was added and homogenized (20 s × 3), and the resulting precipitate was filtered with an eye funnel to remove the solvent. The precipitate was then suspended again in 250 ml of acetone and homogenized (10 s × 3) to obtain a precipitate. The precipitate was washed with acetone and ethyl ether, and then dried to obtain flaxseed acetone powder (150 g).

  The flaxseed acetone powder was suspended in 400 ml of 50 mM phosphate buffer (pH 7.0) under ice cooling, and this was extracted by stirring with a stirrer at 0 ° C. for 1 hour. The obtained extract was centrifuged at 11000 rpm for 30 minutes, and 105.3 g (0-45% saturation) of ammonium sulfate was added to the supernatant (380 ml) thus obtained, and the mixture was allowed to stand for 1 hour under ice cooling, and further 11000 rpm. The precipitate obtained by centrifugation at 30 minutes was dissolved in 150 ml of 50 mM phosphate buffer (pH 7.0), desalted by dialysis (3 L × 3), and crude allen oxide synthase derived from the desired flaxseed seed. An enzyme solution was obtained.

3. Preparation of sodium salt of α-linolenic acid Since α-linolenic acid used as a starting material has a remarkably low solubility in water, α-linolenic acid was sodium-chlorinated in order to facilitate its function as an enzyme substrate. That is, 530 mg of sodium carbonate was dissolved in 10 ml of purified water and heated to 55 ° C., and 278 mg of α-linolenic acid (Nacalai Tesque) was added dropwise thereto and stirred for 3 hours. After completion of the reaction, neutralization was performed with Dawex 50wxk (H ⁺ form) (manufactured by Dow Chemical Co.), and a precipitate was formed. This was filtered to remove the resin, washed with MeOH, and then the solvent was distilled off under reduced pressure. The product thus obtained was recrystallized from isopropanol to obtain the desired sodium salt of α-linolenic acid (1250 mg, 83%).

Production of 4.9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid Sodium salt of α-linolenic acid (15 mg: 50 μmol) obtained in 3 above was added with 0.1 M Dissolved in 30 ml of phosphate buffer (pH 7.0). Next, 3.18 ml of a crude enzyme solution of rice germ-derived lipoxygenase obtained according to 1 above was added to this solution at 25 ° C. in an oxygen stream, and the mixture was stirred for 30 minutes, and then further crude of lipoxygenase derived from rice germ. 3.18 ml of enzyme solution was added and stirred for 30 minutes. After the stirring, 34.5 ml of the crude enzyme solution of allene oxide synthase obtained in 2 above was added to the lipoxygenase reaction product under a nitrogen stream and stirred for 1 hour, and then diluted hydrochloric acid was added under ice cooling. The pH of the reaction solution was adjusted to 3.0.

Then, the reaction solution was extracted with CHCl ₃ -MeOH = 10: 1. Magnesium sulfate was added to the obtained organic layer for dehydration, and the solvent was distilled off under reduced pressure to dry the organic layer. The crude product thus obtained was again subjected to HPLC, and a peak recognized as the specific ketol fatty acid (I) (retention time: around 16 minutes) was collected. Chloroform was added to the collected fraction, the chloroform layer was separated and washed with water, and the chloroform was distilled off with an evaporator to obtain a purified product.

In order to confirm the structure of this purified product, ¹ H and ¹³ C-NMR spectra were measured with a deuterated methanol solution. As a result, in ¹ H-NMR, a terminal methyl group [δ0.98 (t)], two olefins [(δ5.25,5.40), (δ5.55,5.62)], a secondary hydroxyl group [δ4.09 (dd)] and a number of methylene-based signals were observed, presuming the same compound as the specific ketol fatty acid (I).

Furthermore, when the chemical shift value of ¹³ C-NMR was compared, the specific ketol fatty acid (I) [Japanese Patent Application Laid-Open No. 10-324602, page 7, column 11 under the first line and subsequent “production” ¹³ C-NMR chemical shift value in the “example (extraction method)” (described in the paragraph Nos. [0054] and [0055] from the second column on page 8, column 13, column 2). Therefore, the synthesized product obtained by the enzymatic method as described above is 9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid belonging to the specific ketol fatty acid defined above. It became clear that there was.

Preparation of propellant The specific ketol fatty acid is prepared into a propellant by appropriately diluting in pure water so as to have a desired concentration.

Examination of spraying timing of specific ketol fatty acids Using oyster mushrooms and bunshimeji, we investigated when it was most effective to spray specific ketol fatty acids during the cultivation period. In the above propellant, the concentration of 9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid was 30 ppm according to the concentration used by the present inventors in the experiment of the specific ketol fatty acid against carnation. (Yokoyama Yasuyuki, Plant Growth Regulation 40: 90-100, 2005). In addition to the control group in which the spray was not sprayed, the spray was sprayed on any of 3, 10, 14, 21, and 28 days after mycelial inoculation. As a matter of course, the spraying time is the time when the mycelium is growing and no fruiting body appears.

  Subsequently, separately from the test group after the mycelia inoculation, a test group was also provided after the fungus was scraped. The fungi were scraped after the mycelia had grown sufficiently, that is, on the 30th day of the mycelial culture in the case of oyster mushrooms and on the 85th day of the mycelial culture in the case of oyster mushrooms by disturbing the surface of the medium containing the sufficiently grown mycelia. After the fungus scraping step, the spray was sprayed onto the test area. Furthermore, immediately after the primordium begins to form, that is, in the case of Bunashimeji, on the 7th day after fungus scraping, in the case of oyster mushroom, on the third day after fungus scraping, the spray is sprayed in the same manner. Sprayed. The number of bottles used in the test was 32 in all test groups. The results for oyster mushrooms are shown in FIG. 1, and the results for bunashimeji are shown in FIG.

  As is apparent from FIGS. 1 and 2, the suitable time for spraying the specific ketol fatty acid, 9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid used in the present invention is a fungus scraping. Was after. Furthermore, it became clear that when the concentration of the specific ketol fatty acid was increased as revealed in this experiment, the production amount of mushrooms increased by nearly 20%.

1. Determination of optimum concentration of specific ketol fatty acids (9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid) in oyster mushroom and oyster mushroom cultivation Application example of 9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid in practical production of oyster mushroom is a wood-rotting fungus that is distributed all over the world in temperate regions. From the spring to the spring, it occurs in many overlapping hardwood dead trees, fallen trees and stumps. In Japan, facility cultivation mainly using fungus bed bottles is widely performed throughout the year. The spray prepared as described above was applied at the facility cultivation site, and its action was examined.

  A mixture of cedar bark and bran in a ratio of 5: 1 was packed in a plastic bottle (capacity 800 mL), sterilized and allowed to cool in an autoclave, and then inoculated with oyster mushroom seeds. The cells were cultured at 26 ° C. and 75% humidity for 30 days to spread the mycelium throughout. The spray was sprayed after the fungus scraping operation. In the propellant, 9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid was dissolved in water so as to be 0.1, 0.2, 0.5, 1 ppm, About 0.1 mL per atom was sprayed. The control group was not sprayed. In each test section, 55 to 59 bottles were used. After spraying, the spray was transferred to a room at 14 to 16 ° C. and controlled to a humidity of 85%. Nine days later, it seemed that it was in the best time for harvesting (time for shipping), and harvested. The raw weight of oyster mushrooms generated from each bottle was measured, and the average values were compared. The results are shown in FIG.

  As is apparent from FIG. 3, the amount of oyster mushrooms increased depending on the concentration of 9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid. In particular, when the spray concentration was 0.5 ppm, the production amount increased by 10% or more.

2. Application examples of 9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid in the practical production of eringi eringi is a mushroom that originally grows naturally in northern Africa, Central Asia and Europe. It has become popular and has been actively cultivated in Japan. Thus, after the myring of eringi grown by the same culture method as the above-mentioned cultivation of oyster mushrooms, the fungus was scraped, and then the spray was sprayed on the mycelium immediately after the fungus was scraped. The number used in the test was 60 in all test sections. As is clear from FIG. 4, 9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid showed a significant production increase effect at 0.2 ppm. In addition, the production of eringi not only increased, but the fruiting period was accelerated from 10 to 8 days. This can increase the number of times of cultivation when considering the mushroom anniversary cultivation, so that the efficiency of cultivation can be improved from this viewpoint.

  As for other mushrooms, the mycelium growth results by the mushroom cultivation method of the present invention are shown in FIG. As apparent from the results, the mushroom cultivation method of the present invention significantly increased the mycelium of any mushroom. In particular, with regard to Hatake-Shimeji and Ushiratake, the weight of the mycelium increased 1.5 times or more compared to the case where the spray was not sprayed.

Examination of specific ketol fatty acid activity in mushroom mycelium growth Mushroom mycelium culture is a widely used method mainly for mushrooms for health food. The effects of specific ketol fatty acids on mycelial growth were investigated. Oyster mushrooms, Ushiratake mushrooms, eringi, shiitake mushrooms, nameko, maitake mushrooms, and mushroom shimeji mycelia were planted in a 100 ml tall beaker containing 20 ml of SMY medium (1% sucrose, 1% malt extract, 0.4% yeast extract). 9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid was dissolved in 30 nM, 100 nM, and 130 pM in sterile water, and 0.1 mL per petri dish was sprayed from the top after mycelization. 100 nM corresponds to 0.03 ppm. After one month culture at 26 ° C., only fetches the mycelium, shown in FIG. 5 the results of the measurement of the weight.

  Although there is a degree of promotion, 9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid promoted the growth of mushroom mycelium depending on the concentration. In addition, the tendency for the effect of 9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid to promote mycelial growth showed a greater effect for mushrooms that originally had slower mycelial growth. Among the mushrooms shown in FIG. 5, 9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid exhibited a high growth promoting action against Hatake shimeji.

  According to the method of the present invention, by applying the specific ketol fatty acid at a specific time after the fungus scraping step, the mycelium weight of the mushroom can be remarkably increased and the harvest time can also be advanced. In particular, mushrooms with a slow growth rate can benefit from such an increase effect, and therefore the method of the present invention is suitable for such mushrooms that have been slow to grow and difficult to commercialize. In particular, although the present invention is known to have a good taste and a high pharmacological effect, it can be used for Hatake-shimeji mushrooms that have been impeded in full-scale practical use due to slow growth.

FIG. 1 is a graph showing the difference in oyster mushroom fruit body weight (g) depending on the spraying timing of a spray containing a specific ketol fatty acid. FIG. 2 is a graph showing a difference in fruit weight (g) of Bunashimeji depending on the spraying timing of the spray containing the specific ketol fatty acid. FIG. 3 is a bar graph showing optimum concentrations of ketol fatty acids (9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid) in oyster mushroom cultivation. FIG. 4 is a bar graph showing the optimum concentration of ketol fatty acid (9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid) in eringi cultivation. FIG. 5 is a line graph showing the mushroom mycelium growth promoting action of ketol fatty acids (9-hydroxy-10-oxo-12 (Z), 15 (Z) -octadecadienoic acid) on various mushrooms.

Claims (3)

The number of carbon atoms in which the carbon atom in which the carbon atom which comprises a carbonyl group, and the hydroxyl group couple | bonded of the process which carries out a fungus scraping in fungus bed cultivation, and a ketol fatty acid having 4 to 24 carbon atoms is in the α-position A method for cultivating mushrooms comprising a step of applying 4 to 24 ketol fatty acids at a concentration of 0.2 to 0.5 ppm to mycelia after being exposed to stress by the fungus scraping step . The method for cultivating mushrooms according to claim 1 , wherein the application step is performed after the end of the fungus scraping step until the primordium is formed. The cultivation method of the mushrooms of Claim 1 or 2 with which an application process is implemented within 24 hours after completion | finish of a fungus scraping process.

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