JP4964508B2 - Seed disinfectant - Google Patents

Description

  The present invention relates to a seed disinfectant.

  The silver-supported zeolite is an antibacterial zeolite in which part or all of the ion-exchangeable ions in the zeolite are substituted with ammonium ions and / or silver ions (Japanese Patent Laid-Open No. 4-28646, Patent Document 1) "Component (a)"). In addition, silver-supported zeolite has an activity spectrum against nematodes, algae, mosses, and phytopathogenic fungi that damage plants in the field of agriculture and horticulture. It is described in Japanese Patent Application Laid-Open No. 2002-80302 (Patent Document 2) that it exhibits control activity against diseases. However, a high concentration chemical solution is necessary to obtain a stable and high control effect using a chemical solution containing this silver-supported zeolite. If treatment with such a high concentration chemical solution is performed, there is a risk of causing phytotoxicity to crops. There is sex.

On the other hand, methyl-1- (n-butylcarbamoyl) -2-benzimidazole carbamate (hereinafter referred to as “benomyl”), 1,2-bis (3-methoxycarbonyl-2-thioureido) benzene (hereinafter referred to as “thiophanate methyl”). ), (E) -4-chloro-α, α, α-trifluoro-N- (1-imidazol-1-yl-propoxyethylidene) -o-toluidine (hereinafter referred to as “triflumizole”), N—. Propyl-N- {2- (2,4,6-trichlorophenoxy) ethyl} imidazole-1-carboxamide (hereinafter referred to as “prochloraz”), 2-{(4-chlorophenyl) methyl} -5- (1-methyl ethyl) -1- (IH-1,2,4-triazol-1-ylmethyl) - consequent Ropentanoru (hereinafter "ipconazole" And 4- (2,2-difluoro-1,3-benzodioxol-4-yl) pyrrole-3-carbonitrile (hereinafter referred to as “fludioxonil”) are known agricultural and horticultural techniques. It is effective for powdery mildew in various crops. In addition, as a seed disinfectant, it has excellent effects on rice seed blight seedling disease, sesame leaf blight, rice blast, etc., and is widely used as a commercially available pesticide [[Agricultural Chemicals Handbook 2001] (Japan Plant Protection Association 2001) Issued on Nov. 1, 2000), Non-Patent Document 1].

However, so far, it has excellent penetrability to seeds (deeply infected seeds) that have been infected deeply into the seeds, and has better bactericidal activity than conventional copper fungicides. Agricultural and horticultural fungicides that can effectively control all fungal diseases, bacterial diseases, and nematodes (especially rice shinga nematode) diseases, and can effectively sterilize various resistant bacteria at low doses are known. Absent.
JP-A-4-28646 JP 2002-80302 A “Agricultural Chemicals Handbook 2001” (issued on November 1, 2001, Japan Plant Protection Association)

Heretofore, a mixture comprising a benzimidazole fungicide or a sterol biosynthesis inhibitor and thiuram, a copper agent and an oxolinic acid has been widely used as a seed disinfectant. These are known compounds known in the above-mentioned “Agricultural Chemicals Handbook 2001 Edition” (Non-Patent Document 1). However, in recent years, as a result of the continuous use of these mixed agents for many years, benzimidazole compound-resistant fungal seedling fungus, oxolinic acid-resistant rice blast fungus and brown streak fungus have emerged and become a problem.

  In addition, for phlezoate and prochloraz, which are one of the sterol biosynthesis inhibitors, there is no known low-sensitivity bacterium with poor control effect to date, but triflumizole is a low-sensitivity rice sapling fungus with low control effect. There is an outbreak report. Further, the copper disinfectant has a problem that the disinfecting power is not so strong, and a large amount of the disinfectant causes phytotoxicity depending on conditions. As described above, it is difficult to say that conventional seed disinfectants are necessarily satisfactory in terms of effects, drug-resistant bacteria, and safety of phytotoxicity, and development of excellent drugs with few such problems is desired.

  The present invention is intended to solve such problems associated with the prior art, has excellent permeability to deeply infected seeds, etc., has better bactericidal power than conventional copper fungicides, and is capable of transmitting seeds. Agricultural and horticultural fungicide (new seed disinfectant) that can effectively control all fungal diseases, bacterial diseases, and nematode (especially rice shinga nematode) diseases, and can effectively sterilize various resistant bacteria at low doses. ).

  In order to achieve the above-mentioned object, the present inventors have achieved a low-dose seed disinfectant that can exert stable effects on seed-borne fungi, bacterial diseases, nematode diseases, etc. We intensively studied to develop the agent.

  As a result, if two types of silver-carrying zeolite and a specific agricultural and horticultural fungicide are used in combination, it is possible to effectively control major seed-borne fungal diseases, bacterial diseases and nematode diseases, The inventors have found that a synergistic effect is obtained against filamentous fungi and that a very high seed disinfection effect can be exhibited at a low concentration, and the present invention has been completed.

That is, the gist of the present invention is that
(a) a silver-carrying zeolite;
(b) Methyl-1- (n-butylcarbamoyl) -2-benzimidazole carbamate (“benomyl”), 1,2-bis (3-methoxycarbonyl-2-thioureido) benzene (“thiophanate methyl”), (E) -4-chloro-α, α, α-trifluoro-N- (1-imidazol-1-yl-propoxyethylidene) -o-toluidine (“triflumizole”), N-propyl-N- {2- ( 2,4,6-trichlorophenoxy) ethyl} imidazole-1-carboxamide (“prochloraz”), 2-{(4-chlorophenyl) methyl} -5- (1-methylethyl) -1- (1H-1,2, , 4-triazol-1-ylmethyl) - consequent Ropentanoru ( "ipconazole"), and 4- (2,2-difluoro-1,3-Benzojio At least one selected from among the sole 4-yl) pyrrole-3-carbonitrile ( "fludioxonil"),
And a seed disinfectant characterized by comprising

  In the present invention, the silver ion content in the silver-supported zeolite (a) is preferably 0.1 to 15.0% by weight, and with respect to 100 parts by weight of the silver-supported zeolite (a), The content of the component (b) is desirably 1 to 2000 parts by weight.

  The seed disinfectant according to the present invention is suitable for rice fungal diseases, bacterial diseases and nematode diseases.

According to the present invention, it has excellent penetrability to deeply infected seeds and the like, and is superior in bactericidal activity compared to commercially available copper fungicides. Seed infectious fungal diseases, bacterial diseases and nematodes (especially rice gingale nematodes) ) Agricultural and horticultural fungicides (new seed disinfectants) that can effectively control all diseases and effectively kill various resistant bacteria at low doses are provided.

Hereinafter, the seed disinfectant of the present invention and the labor-saving control method for rice diseases using the same will be described in detail.
[Seed disinfectant]
The seed disinfectant according to the present invention includes, as essential active ingredients, silver-supported zeolite (a) and component (b), that is, methyl-1- (n-butylcarbamoyl) -2-benzimidazole carbamate (“benomyl”). "),
1,2-bis (3-methoxycarbonyl-2-thioureido) benzene ("thiophanate methyl"),
(E) -4-chloro-α, α, α-trifluoro-N- (1-imidazol-1-yl-propoxyethylidene) -o-toluidine (“triflumizole”),
N-propyl-N- {2- (2,4,6-trichlorophenoxy) ethyl} imidazole-1-carboxamide ("Prochloraz"),
2 - {(4-chlorophenyl) methyl} -5- (1-methylethyl) -1- (IH-1,2,4-triazol-1-ylmethyl) - consequent Ropentanoru ( "ipconazole") and,
At least one selected from 4- (2,2-difluoro-1,3-benzodioxol-4-yl) pyrrole-3-carbonitrile (“fludioxonil”);
And are contained. The seed disinfectant according to the present invention contains the component (a) and the component (b) as essential components, but the active ingredients that can be blended are not limited thereto.
<(A) Silver supported zeolite>
The silver-carrying zeolite (a) contained in the seed disinfectant according to the present invention is obtained by substituting a part or all of the ion-exchangeable ions in the zeolite with silver ions (JP-A-4-28646). As a zeolite used, any of natural zeolite and synthetic zeolite can be used.

Zeolites is generally aluminosilicate having a three dimensional framework structure, represented by _{XM 2 / n O · Al 2} O 3 · YSiO 2 · ZH 2 O as a general formula. Here, M represents an ion capable of ion exchange, and is usually a monovalent or divalent metal ion. n is the valence of (metal ion). X and Y represent the respective metal oxides and silica coefficients, and Z represents the number of crystal water.

  Specific examples of the zeolite include, for example, A-type zeolite, X-type zeolite, Y-type zeolite, T-type zeolite, high silica zeolite, sodalite, mordenite, analcite, clinoptilolite, chiapasite, erionite and the like. Can be mentioned. However, it is not limited to these.

The amount of silver ions to be supported on the zeolite is the content of silver ions in the silver-supported zeolite (a) from the viewpoint of safety against phytotoxicity to rice [measurement method: measured value by either atomic absorption method or fluorescent X-ray method, preferably Is preferably 0.1 to 15% by weight, more preferably 1 to 10% by weight. Furthermore, the content of the silver-carrying zeolite in the preparation is 10 to 70% by weight, preferably 30 to 50% by weight, taking into consideration the safety of phytotoxicity and the workability of the preparation.
(Preparation of silver supported zeolite)
In order to prepare a silver-supported zeolite, a preliminarily prepared “aqueous solution containing silver ions” (silver ion-containing solution) is brought into contact with the zeolite to replace the ion-exchangeable ions and silver ions in the zeolite. (Ion exchange) may be performed. The contact can be carried out at a temperature of usually 10 to 70 ° C., preferably 40 to 60 ° C., usually for 3 to 24 hours, preferably about 10 to 24 hours, batchwise or continuously (for example, column method). In addition, it is preferable to adjust pH of the said aqueous solution to 3-10 normally, Preferably it is 5-7. It is preferable to carry out the ion exchange reaction after adjusting to such a pH value because silver oxide or the like can be prevented from precipitating on the zeolite surface or in the pores thereof.

  In addition, a salt is normally used as a silver ion supply source in the aqueous solution. For example, silver nitrate, silver sulfate, silver perchlorate, silver acetate, diammine silver nitrate, diammine silver sulfate and the like can be used.

  The content of silver ions in the zeolite can be appropriately controlled by adjusting the silver ion (salt) concentration in the aqueous solution. For example, as the silver ion content of the silver-supported zeolite, the silver ion content is 0.1 to 5% by adjusting the silver ion concentration in the aqueous solution to 0.002 M / liter to 0.15 M / liter. A supported zeolite can be obtained.

After the above ion exchange, the obtained silver-supported zeolite contains water, an excessive amount of silver ions, a salt and the like, and is usually washed thoroughly with water and then dried. The drying is preferably performed by heating at a temperature of about 105 to 115 ° C. under normal pressure, or by heating at a temperature of about 70 to 90 ° C. under reduced pressure (eg, 1 to 30 torr). .
<Component (b)>
As component (b), methyl-1- (n-butylcarbamoyl) -2-benzimidazole carbamate, 1,2-bis (3-methoxycarbonyl-2-thioureido) benzene, (E) -4-chloro-α , Α, α-trifluoro-N- (1-imidazol-1-yl-propoxyethylidene) -o-toluidine, N-propyl-N- {2- (2,4,6-trichlorophenoxy) ethyl} imidazole- 1-carboxamide, 2 - {(4-chlorophenyl) methyl} -5- (1-methylethyl) -1- (IH-1,2,4-triazol-1-ylmethyl) - sik Ropentanoru, 4- (2, And one or more compounds selected from 2-difluoro-1,3-benzodioxol-4-yl) pyrrole-3-carbonitrile. . In the present invention, the compounds contained in these components (b) are used singly or in combination of two or more.

  These components (b) are known agricultural and horticultural fungicides listed in the “Agricultural Chemicals Handbook 2001 Edition” (Non-Patent Document 1) mentioned in the section “Background Art”, and are themselves seeds. As a disinfectant, it has excellent effects on rice seed potato batter seedlings, sesame leaf blight, blast, etc., and is widely used as a commercially available pesticide.

In the seed disinfectant of the present invention, the component (b) is usually contained in an amount of 1 to 2000 parts by weight, preferably 5 to 200 parts by weight, with respect to 100 parts by weight of the silver-carrying zeolite (a). It is desirable from the viewpoint of seed disinfection effect against various diseases.
<Preparation of seed disinfectant (formulation method)>
The seed disinfectant of the present invention comprises at least two active ingredients of the above-mentioned silver-carrying zeolite (a) and the above-mentioned ingredient (b), or the active ingredients separately from a suitable carrier and auxiliary agent such as a surface active agent. One-part seed disinfectant or two-part seed by blending with agents, binders, stabilizers, etc. and formulating into wettable powders, emulsions, flowables, hydrated granules, emulsions, etc. according to conventional methods Can be manufactured as a disinfectant.

  The mixing ratio of the active ingredient in the present invention is a weight ratio, and the component (b) is usually added in an amount of 1 to 2000 parts by weight, preferably 5 to 200 parts by weight with respect to 100 parts by weight of the silver-supported zeolite (a). Is preferred. However, the blending ratio can be appropriately changed depending on the conditions at the time of use.

As the carrier that can be used, any solid or liquid can be used as long as it is commonly used as a bulking agent, an excipient or the like in an agrochemical formulation, and is not limited to a specific one.
For example, as solid carriers, mineral powders (kaolin, bentonite, clay, talc, diatomaceous earth, silica, vermiculite, calcium carbonate, etc.), natural polymers (wheat flour, starch, crystalline cellulose, carboxymethylcellulose, gelatin, etc.), sugars (Glucose, maltose, lactose, sucrose, etc.), ammonium sulfate, urea and the like.

  Liquid carriers include water, ethyl alcohol, ethylene glycol, propylene glycol, benzyl alcohol, benzene, toluene, xylene, methylnaphthalene, acetone, methyl ethyl ketone, cyclohexanone, ethyl acetate, butyl acetate, ethylene glycol acetate, propylene glycol monomethyl ether , Dipropylene glycol monomethyl ether, hexane, petroleum ether, solvent naphtha, kerosene, light oil and the like.

  In the preparation of wettable powders, flowables, emulsions and the like, surfactants or emulsifiers are used for the purpose of emulsification, dispersion, solubilization, wetting, foaming, spreading and the like. Examples of such surfactants include nonionic, anionic, cationic, and amphoteric types such as those described in [0011] to [0014] of Japanese Patent Publication No. 2002-80313. .

  Specifically, for example, polyoxyethylene nonylphenyl ether, sodium lignin sulfonate, polyoxyethylene castor oil ether, polyoxyethylene / polyoxypropylene block polymer, sodium lauryl sulfate and the like are used.

In addition to these, various adjuvants such as antioxidants, photodegradation inhibitors, physical property improvers, active ingredient stabilizers, viscosity modifiers, antifreeze agents and the like can be used.
Further, the seed disinfectant of the present invention may contain other bactericidal components, insecticidal components, plant growth regulating components, and these may be blended with the above-described components as necessary when preparing the seed disinfectant. It can be used by mixing.
A wettable powder, for example, in a wettable powder , polyoxyethylene nonylphenyl ether, polyoxyethylene alkyl ether in an amount of 1 to 2000 parts by weight of component (b) with respect to 100 parts by weight of the above-mentioned silver-supported zeolite (a). 1 to 1000 parts by weight of a surfactant or an emulsifier such as sodium lignin sulfonate, and 1 to 1000 parts by weight of a solid carrier such as white carbon or clay, and an extender in an amount of 1 to 1000 parts by weight. Alternatively, the desired wettable powder can be obtained by blending little by little in any order, mixing and grinding until uniform.

The content of the silver-supported zeolite (a) having the above silver ion content in such a wettable powder is usually 10 to 70% by weight, preferably about 30 to 50% by weight. In such wettable powder, it is desirable to adjust the hydration property within 3 minutes or 2 minutes and the apparent specific gravity to 0.4 or more.
Flowable agent For example, in the flowable agent, the component (b) is added in an amount of 5 to 200 parts by weight with respect to 100 parts by weight of the silver-carrying zeolite (a) having the silver ion content, polyoxyethylene nonylphenyl ether, polyoxy Surfactant or emulsifier such as ethylene alkyl ether or sodium lignin sulfonate in an amount of 1 to 1000 parts by weight, thickener such as xanthan gum in an amount of 0.1 to 1000 parts by weight, and water in an amount of 100 to 10000 (1 The desired flowable agent can be obtained by using them in an amount of 10 parts by weight, mixing them little by little in any order or mixing and dispersing until uniform.

The content of the silver-supported zeolite in such a flowable agent is usually 10 to 60% by weight, preferably about 30 to 50% by weight.
[Rice disease control method]
As the disinfecting method using the seed disinfectant according to the present invention, (i): the seed disinfectant of the present invention is diluted 10 to 1000 times with water, and the amount of the obtained diluted solution is 0.7 to 4 A method of immersing a double amount (volume) of rice seed bran in the diluted solution, or (b): using powdered wettable powder as it is without diluting, and corresponding to 0.1 to 4.0% by weight of the seed bran Method of dressing seed pods with powdered wettable powder, (c): Spraying a 5 to 40-fold diluted solution of the above seed disinfectant using a dedicated spraying device so as to be equivalent to 3% of the seed pod weight The method of doing is mentioned.

In the seed immersing method in the seed disinfectant diluted solution (b) above, the seed pods are contained in a low-concentration chemical solution (example: 100
If it is immersed for a long time (eg, about 6 to 72 hours) in high-concentration chemical solution (eg, about 10 to 100 times dilution) (for about 10 to 30 minutes) Good.

  The seed disinfectant of the present invention includes a first component containing component (a) (silver-supported zeolite) and component (b) (that is, any one of benomyl, thiophanate methyl, triflumizole, prochloraz, ipconazole, fludioxonil). A two-component preparation comprising a second component containing two or more species), or a commercially available single agent (single agent (a)) containing component (a) and component ( Using a single agent containing b) (single agent (b)), etc., after immersing the seed in one single agent (a) and then treating the seed, the other agent (single agent (b)) is further immersed. Proximity treatment such as treatment, and each single agent (a), (b) is mixed in a predetermined amount ratio, seeds are immersed in this, or the seeds are simultaneously treated by powdering and spraying. Is obtained.

As conditions for soaking in water, for example, about 10 to 15 ° C. [Example: 15 ° C.] Water × 6 to 9 days [Example: 6 days] is preferable.
Moreover, as germination treatment conditions, for example, about 30 to 32 ° C. [Example: 32 ° C.] × 15 to 18 hours [Example: 15 hours] is desirable.

The above rice disease control method is suitable when the rice disease is a filamentous fungal disease, a bacterial disease, a rice shingle nematode disease, or the like as described above.
Hereinafter, the method for controlling rice diseases will be described in more detail.

  In the present invention, the method for treating seed pods with the above-mentioned drug (seed disinfectant) is performed as follows. That is, in the powder dressing method, rice seed meal and seed disinfectant are placed in a rotary drum, and the drum is rotated so that the medicine is uniformly coated on the seed meal. For example, (1) a method of spraying a chemical directly using a nozzle suitable for seeds falling from the hopper, (2) a vibrating guide plate is attached from the hopper to the nursery box, For example, a method of spraying a chemical solution using a suitable nozzle when the seed pod passes through, (3) a method of attaching a drum that leads from the hopper to the seedling box and spraying the chemical solution to the seed pod passing through the drum can be used.

In addition, the above-mentioned spraying machine is provided with a chemical receiver that collects the chemical liquid that has not been sprayed on the seed pod, and is returned to the chemical tank by the pump. As a method for spraying a chemical solution to a small-scale seed pod, the seed pod is put in a rotating machine such as a mortar mixer, and a predetermined amount of medicine is sprayed uniformly with an appropriate sprayer.
[Effect of the invention]
Firstly, the seed disinfectant of the present invention includes a silver-supported zeolite (a) and the specific component (b) (that is, any one or two of benomyl, thiophanate methyl, triflumizole, prochloraz, ipconazole, fludioxonil). 2 types of active ingredients are used in combination, so it is highly effective against rice sapling, rice blast, and sesame leaf blight, especially against rice blast and sesame leaf blight Synergistic effects are recognized, and it also shows a high control effect against rice seedling blight, rice blight and brown streak.

  The silver-carrying zeolite (a) contained in the seed disinfectant of the present invention is widely known that silver ions have a strong bactericidal effect, and the eluted silver ions exhibit an antibacterial action non-selectively. it is conceivable that. On the other hand, benzimidazole fungicides such as benomyl and thiophanate methyl contained in component (b), which is another essential component, are thought to inhibit mitotic fission by binding to microtubule proteins of pathogenic bacteria. ["Agricultural Chemicals Handbook 2001 Edition" (issued on November 1, 2001, Japan Plant Protection Association, page 252)].

In addition, triflumizole, prochloraz, and ipconazole contained in component (b) inhibit the biosynthesis of ergosterol, which is an essential component contained in the cell membrane of pathogenic bacteria, and act by losing membrane function. ["Agricultural Chemicals Handbook 2001 Edition" (issued on November 1, 2001 by the Japan Plant Protection Association) page 274].
Furthermore, fludioxonil is also considered to act on the cell membrane of pathogenic bacteria ["Agricultural chemical handbook 2001 version" (issued on November 1, 2001, Japan Society for Plant Protection) page 328].

In the present invention, by combining (mixing) these two active ingredients (a) and (b) having different action characteristics, a high synergistic effect is exhibited, and the rice seedling disease, rice blast disease It is thought that it has led to an excellent seed disinfection effect against sesame leaf blight.
Secondly, the seed disinfectant of the present invention shows an effect as high as that against oxolinic acid-resistant rice blast fungus and brown streak fungus as well as against oxolinic acid-sensitive rice fever bacterial fungus and brown streak fungus.

Thirdly, the seed disinfectant of the present invention is highly effective against rice gingale nematode disease.
[Example]
Next, the seed disinfectant and the labor-saving control method using the seed disinfectant according to the present invention will be described more specifically with reference to examples, but the present invention is not limited to these examples.

In addition, silver ion content in the component (a) (silver carrying zeolite) used in the following examples and test examples is 4.45% (% by weight). In the examples, all parts are parts by weight.
<Examples of preparation and test of seed disinfectant>
[Example 1 (wettable powder)]
(1) Silver The carrier prepared zeolite of the zeolite (a1) A- type zeolite _{(Na 2 O · Al 2 O} 3 · 1.9SiO 2 · XH 2 O: average particle size 1.5 [mu] m, trade name: "Zeomic" (Sinen Zeomic Co., Ltd.) was used, and AgNO ₃ was used as a salt for providing silver ions.

  Water is added to 1 kg of the above zeolite powder heated and dried at 110 ° C. to make a slurry of 1.3 liters, and then stirred and degassed. Further, an appropriate amount of 0.5N nitric acid solution and water are added to adjust the pH to 6. To a 1.8 liter slurry.

Next, for silver ion exchange, 3 liters of a 0.15M silver nitrate solution is added to make a total volume of 4.8 liters, and this slurry is kept at 40-60 ° C. and is brought into an equilibrium state while stirring for 10-24 hours. Kept in the reached state. After completion of the ion exchange, the zeolite phase was filtered and washed with room temperature water or warm water until there was no excess silver ion in the zeolite phase. This was heated and dried at 110 ° C. to obtain a silver-supported zeolite sample (referred to as silver-supported zeolite (a1) and component (a1)) having a silver loading of 4.45%.
(2) 50 parts of the above component (a1) (silver-supported zeolite), 20 parts of benomyl, 2 parts of polyoxyethylene alkyl ether and 28 parts of clay as the above component (b) are uniformly mixed and pulverized to obtain a wettable powder. Obtained.
[Example 2 (wettable powder)]
35 parts of thiophanate methyl, 50 parts of the above-mentioned silver-supported zeolite (a1), 1 part of polyoxyethylene alkyl ether, 3 parts of sodium lignin sulfonate and 11 parts of clay were uniformly mixed and ground to obtain a wettable powder.
[Example 3 (wettable powder)]
20 parts of triflumizole, 50 parts of the above-mentioned silver-supported zeolite (a1), 1 part of polyoxyethylene alkyl ether, 5 parts of calcium lignin sulfonate and 24 parts of clay were uniformly mixed and pulverized to obtain a wettable powder.
[Example 4 (wettable powder)]
5 parts of prochloraz, 50 parts of the above-mentioned silver supported zeolite (a1), 2 parts of polyoxyethylene alkyl ether, 3 parts of sodium lignin sulfonate, 3 parts of white carbon, clay
37 parts were mixed uniformly and pulverized to obtain a wettable powder.
[Example 5 (wettable powder)]
5 parts of ipconazole, 50 parts of the above-mentioned silver-supported zeolite (a1), 2 parts of polyoxyethylene alkyl ether and 45.5 parts of clay were uniformly mixed and ground to obtain a wettable powder.
[Example 6 (wettable powder)]
2.5 parts of fludioxonil, 50 parts of the above-mentioned silver-supported zeolite (a1), 1 part of polyoxyethylene alkyl ether, 3 parts of sodium lignin sulfonate and 11 parts of clay were uniformly mixed and pulverized to obtain a wettable powder.

Seed disinfection treatment was performed using a preparation prepared according to the above preparation examples (Examples 1 to 6). [Test example]
Next, the seed disinfection effect of the seed disinfectant according to the present invention will be described with reference to test examples.
<Test Example 1> (Rabbit seedling disease control effect test)
Infected pods (variety “Niigata Hayao”) inoculated with benzimidazole compound-sensitive bacteria at the flowering stage were sterilized by the following method (i), (ii) or (iii).
(I) In the immersion treatment method, 150 g of seed pods were immersed in 270 ml of a seed disinfectant (chemical solution) according to the present invention at a predetermined concentration for 24 hours and then dried in the shade for 6 hours.
(Ii) In the powder dressing method, 150 g of seed meal and a predetermined amount of the seed disinfectant according to the present invention were put in an Erlenmeyer flask and powdered.
(Iii) Further, in the spraying method, the seed disinfectant according to the present invention is diluted with water to 150 g of seed meal, and 4.5 ml of a chemical solution having a predetermined concentration (equivalent to 3% of the seed meal weight) is evenly distributed in the seed seed. It sprayed using the small air sprayer so that it might adhere.

This chemical-treated cocoon was soaked in tap water twice as much as the seed potato volume at 15 ° C. for 5 days.
Thus, the amount of 15 g out of 150 g of the seed pods each treated with a medicine is used as a seedling box (vertical x horizontal x horizontal), which is 1/10 the size of a normal nursery box (vertical x horizontal x height = 60 cm x 30 cm x 3 cm). Height = 12 cm × 15 cm × 3 cm) and immediately covered with soil. After covering the soil, germination was carried out by holding at 32 ° C. for 2 days. After the emergence, the seedling was shielded from light with a cold cocoon in the greenhouse, after which the cold cocoon was removed and the seedlings were managed in a glass greenhouse.

In addition, as the seedling culture soil, commercially available “Kumiai Synthetic Culture soil No. 3” (manufactured by Mitsui Toatsu Fertilizer Co., Ltd.) was used.
30 days after sowing, all seedlings in the nursery box were examined for the number of diseased seedlings and diseaseless seedlings that showed symptoms of rice seedling disease such as chief, withering, etc., and the diseased seedling rate (%) by the following formula [Equation 1] And the control value (%) was obtained from [Equation 2]. Regarding phytotoxicity, the degree of emergence, growth, etc. were observed and indicated as the following phytotoxicity.

(Degree of chemical damage)-: None, ±: Slight, +: Small, ++: Medium, +++: Large.
The results are shown in Table 1.

<Test Example 2> (Rice effect control for rice blast)
Naturally infected rice cakes (variety: Koshihikari) collected from a field with frequent rice blast were sterilized by the following method. In the dipping treatment method, 150 g of seed pods were dipped in 270 ml of a chemical solution having a predetermined concentration for 24 hours and then shaded for 6 hours. In the dressing treatment method, 150 g of seed meal and a predetermined amount of a commercially available drug were placed in an Erlenmeyer flask and dressed. In addition, in the spraying method, a commercially available drug is diluted with water for 150 g of seed meal, and 4.5 ml of a chemical solution (equivalent to 3% of the weight of the seed meal) is uniformly attached to the seed meal. Spraying was performed using a player. This drug-treated koji was soaked at 15 ° C. for 5 days in tap water twice the volume of the koji.

  Thus, the amount of 15 g out of 150 g of the seed pods treated with each of the chemicals is increased to 1/10 of a normal seedling box (vertical x horizontal x height = 60 cm x 30 cm x 3 cm). S = 12 cm × 15 cm × 3 cm). Thereafter, budding treatment was carried out at 20 ° C. for 4 days under uncovered soil conditions. After 2 days of budding, the seedlings were shielded from light with a cold cocoon in the greenhouse, and thereafter, the chilled cocoons were removed and the seedlings were managed in a glass greenhouse.

In addition, the commercially available “Kumiai Synthetic Soil No. 3” (manufactured by Mitsui Toatsu Fertilizer Co., Ltd.) was used as the seedling culture soil.
30 days after sowing, the number of diseased seedlings and the number of diseaseless seedlings were investigated for all seedlings in the nursery box, and the diseased seedling rate (%) was obtained by the following formula [Equation 3], and the control value (%) was obtained by [Equation 4]. Asked. Regarding phytotoxicity, the degree of emergence, growth, etc. were observed and indicated as the following phytotoxicity.

  The results are shown in Table 2.

(Drug damage degree)
-: None,
±: Fine,
+: Small,
++: Medium,
+++: Large.

<Test Example 3> (Rice sesame leaf blight control effect test)
Infected pods (variety: Kinuhikari) inoculated with rice sesame leaf blight fungus at the flowering stage were sterilized by the following method. In the dipping treatment method, 150 g of seed pods were dipped in 270 ml of a chemical solution having a predetermined concentration for 24 hours and then shaded for 6 hours. In the dressing treatment method, 150 g of seed meal and a predetermined amount of a commercially available drug were placed in an Erlenmeyer flask and dressed. In addition, in the spraying method, a commercially available drug is diluted with water for 150 g of seed meal, and 4.5 ml of a chemical solution (equivalent to 3% of the weight of the seed meal) is uniformly attached to the seed meal. Spraying was performed using a player. This drug-treated koji was soaked at 15 ° C. for 5 days in tap water twice the volume of the koji.

Thus, the amount of 15 g out of 150 g of the seed pods treated with each of the chemicals is increased to 1/10 of a normal seedling box (vertical x horizontal x height = 60 cm x 30 cm x 3 cm). S = 12 cm × 15 cm × 3 cm) and immediately covered with soil. Then, after covering, the seedling was treated for 2 days at 32 ° C., and after 2 days of germination, the seedling was shielded from light with a cold cocoon in the greenhouse, after which the cold cocoon was removed and the seedlings were managed in a glass greenhouse.
In addition, the commercially available "Kumiai Synthetic Culture No. 3" (manufactured by Mitsui Toatsu Fertilizer Co., Ltd.) was used as the seedling cultivation soil.

  30 days after sowing, all seedlings in the seedling box were examined for the number of rice sesame seedlings and healthy seedlings according to the following survey criteria, and the degree of disease was determined by the following equation [Equation 5]. The control value (%) was determined. Regarding phytotoxicity, the degree of emergence, growth, etc. were observed and indicated as the following phytotoxicity.

The results are shown in Table 3.
(Investigation criteria for pathogenesis)
N0: the number of seedlings that are not sick,
N1: the number of seedlings that are affected in the sheath leaf,
N3: the number of seedlings that are diseased in the first leaf
N5: Number of seedlings with extremely poor growth due to disease.

(Drug damage degree)
-: None, ±: Slight, +: Small, ++: Medium, +++: Large.

<Test Example 4> (Rice Bacterial Disease Control Effect Test)
Infected pods (variety: Kinuhikari) inoculated with rice seedling blight fungus at the flowering stage were sterilized by the following method. In the dipping treatment method, 150 g of seed pods were dipped in 270 ml of a chemical solution having a predetermined concentration for 24 hours and then shaded for 6 hours. In the dressing treatment method, 150 g of seed meal and a predetermined amount of a commercially available drug were placed in an Erlenmeyer flask and dressed. In addition, in the spraying method, a commercially available drug is diluted with water for 150 g of seed meal, and 4.5 ml of a chemical solution (equivalent to 3% of the weight of the seed meal) is uniformly attached to the seed meal. Spraying was performed using a player. This drug-treated koji was soaked at 15 ° C. for 5 days in tap water twice the volume of the koji.

Thus, the amount of 15 g out of 150 g of the seed pods treated with each of the chemicals is increased to 1/10 of a normal seedling box (vertical x horizontal x height = 60 cm x 30 cm x 3 cm). S = 12 cm × 15 cm × 3 cm) and immediately covered with soil. Then, after covering, the seedling was treated for 2 days at 32 ° C., and after 2 days of germination, the seedling was shielded from light with a cold cocoon in the greenhouse, after which the cold cocoon was removed and the seedlings were managed in a glass greenhouse.
In addition, the commercially available “Kumiai Synthetic Soil No. 3” (manufactured by Mitsui Toatsu Fertilizer Co., Ltd.) was used as the seedling culture soil.

30 days after sowing, all the seedlings in the seedling box were examined for the number of rice seedlings and the number of healthy seedlings according to the following survey criteria, and the degree of disease was determined by the following equation [Equation 7], [Equation 8] The control value (%) was determined by Regarding phytotoxicity, the degree of emergence, growth, etc. were observed and indicated as the following phytotoxicity.
(Investigation criteria for pathogenesis)
N0: the number of seedlings that are not sick,
N1: number of seedlings where whitening is observed in the second leaf,
N2: The number of seedlings in which the first leaf is whitened and growth inhibition is observed,
N3: remarkable growth suppression or number of dead seedlings.

(Degree of chemical damage)-: None, ±: Slight, +: Small, ++: Medium, +++: Large.

<Test Example 5> (Rice blast fungus control effect test)
As a test rice cake, rice (variety: Kinuhikari) rice that was obtained by inoculating rice suspensions of rice blast bacteriomycete oxolinic acid-sensitive bacteria and resistant bacteria (1 × 10 ⁸ CFU / ml) under reduced pressure. Rice cake with bacterial wilt disease was used. The seeds were sterilized by the following method. In the dipping treatment method, 150 g of seed pods were dipped in 270 ml of a chemical solution having a predetermined concentration for 24 hours and then shaded for 6 hours. In the dressing treatment method, 150 g of seed meal and a predetermined amount of a commercially available drug were placed in an Erlenmeyer flask and dressed. In addition, in the spraying method, a commercially available drug is diluted with water for 150 g of seed meal, and 4.5 ml of a chemical solution (equivalent to 3% of the weight of the seed meal) is uniformly attached to the seed meal. Spraying was performed using a player. This drug-treated koji was soaked at 15 ° C. for 5 days in tap water twice the volume of the koji.

The amount of 15 g out of 150 g of the seed pods treated with the drug in this way was added to a normal nursery box (vertical ×
A seedling box (vertical × horizontal × height = 12 cm × 15 cm × 3 cm) having a size one tenth of horizontal × height = 60 cm × 30 cm × 3 cm was seeded and immediately covered with soil. Then, after covering, the seedling was treated for 2 days at 32 ° C., and after 2 days of germination, the seedling was shielded from light with a cold cocoon in the greenhouse, after which the cold cocoon was removed and the seedlings were managed in a glass greenhouse.
In addition, the commercially available “Kumiai Synthetic Soil No. 3” (manufactured by Mitsui Toatsu Fertilizer Co., Ltd.) was used as the seedling culture soil.

  30 days after sowing, the number of seedlings and the number of healthy seedlings of rice blast fungus disease were investigated according to the following survey criteria for all seedlings in the seedling box, and the disease severity was determined by the following equation [Equation 9]. The control value (%) was determined. Regarding phytotoxicity, the degree of emergence, growth, etc. were observed and indicated as the following phytotoxicity.

The results are shown in Table 5.
(Investigation criteria for pathogenesis)
N0: the number of seedlings that are not sick,
N1: number of seedlings where whitening is observed in the second leaf,
N2: The number of seedlings in which the first leaf is whitened and growth inhibition is observed,
N3: The number of seedlings with significant growth failure or rot.

(Degree of chemical damage)-: None, ±: Slight, +: Small, ++: Medium, +++: Large.

<Test Example 6> (Rice brown disease control effect test)
The rice bran was obtained by inoculating rice (variety: Kinuhikari) rice bran with oxalinic acid-sensitive and resistant bacterial suspensions (1 × 10 ⁸ CFU / ml) of rice brown streak. A streak-infected moth was used. The seeds were sterilized by the following method. In the dipping treatment method, 150 g of seed pods were dipped in 270 ml of a chemical solution having a predetermined concentration for 24 hours and then shaded for 6 hours. This chemical-treated cocoon was soaked in tap water twice as much as the seed potato volume at 15 ° C. for 5 days.

Thus, the amount of 15 g out of 150 g of the seed pods treated with each of the chemicals is increased to 1/10 of a normal seedling box (vertical x horizontal x height = 60 cm x 30 cm x 3 cm). S = 12 cm × 15 cm × 3 cm) and immediately covered with soil. Then, after covering, the seedling was treated for 2 days at 32 ° C., and after 2 days of germination, the seedling was shielded from light with a cold cocoon in the greenhouse, after which the cold cocoon was removed and the seedlings were managed in a glass greenhouse.
In addition, the commercially available “Kumiai Synthetic Soil No. 3” (manufactured by Mitsui Toatsu Fertilizer Co., Ltd.) was used as the seedling culture soil.

30 days after sowing, the number of rice brown streak diseased seedlings and the number of healthy seedlings were investigated according to the following survey criteria for all seedlings in the seedling box, and the disease severity was determined by the following formula [Equation 11] and controlled from [Equation 12]. The value (%) was determined. Regarding phytotoxicity, the degree of emergence, growth, etc. were observed and indicated as the following phytotoxicity.

The results are shown in Table 6.
(Investigation criteria for pathogenesis)
N0: the number of seedlings that are not sick,
N1: the number of seedlings in which disease is found in the incomplete leaves,
N2: the number of seedlings where disease is observed in the first leaf,
N3: Number of seedlings withered or diseased on the second leaf.

(Degree of chemical damage)-: None, ±: Slight, +: Small, ++: Medium, +++: Large.

<Test Example 7> (Relative nematode control effect test)
As the test pods, rice scentless nematode naturally infected pods were used as rice (variety: Koshihikari) pods. The seeds were sterilized by the following method. In the dipping treatment method, 150 g of seed pods were dipped in 270 ml of a chemical solution having a predetermined concentration for 24 hours and then shaded for 6 hours. This chemical-treated cocoon was soaked in tap water twice as much as the seed potato volume at 15 ° C. for 5 days.

Thus, the amount of 15 g out of 150 g of the seed pods treated with each of the chemicals is increased to 1/10 of a normal seedling box (vertical x horizontal x height = 60 cm x 30 cm x 3 cm). S = 12 cm × 15 cm × 3 cm) and immediately covered with soil. Then, after covering, the seedling was treated for 2 days at 32 ° C., and after 2 days of germination, the seedling was shielded from light with a cold cocoon in the greenhouse, after which the cold cocoon was removed and the seedlings were managed in a glass greenhouse.
In addition, the commercially available “Kumiai Synthetic Soil No. 3” (manufactured by Mitsui Toatsu Fertilizer Co., Ltd.) was used as the seedling culture soil.

The seedlings 28 days after sowing were transplanted into paddy fields and harvested by a conventional method during the harvesting period.
Threshing is performed after harvesting, and the number of damaged grains is investigated for 5000 threshed grains. The percentage of damaged grains (%) is obtained from the following formula [Equation 13], and the control value (%) is obtained from the following formula [Equation 14]. Calculated.

  The results are shown in Table 7.

(Degree of chemical damage)-: None, ±: Slight, +: Small, ++: Medium, +++: Large.

Claims (3)

(a) a silver-carrying zeolite;
(b) Methyl-1- (n-butylcarbamoyl) -2-benzimidazole carbamate, 1,2-bis (3-methoxycarbonyl-2-thioureido) benzene, (E) -4-chloro-α, α, α -Trifluoro-N- (1-imidazol-1-yl-propoxyethylidene) -o-toluidine, N-propyl-N- {2- (2,4,6-trichlorophenoxy) ethyl} imidazole-1-carboxamide, 2 - {(4-chlorophenyl) methyl} -5- (1-methylethyl) -1- (IH-1,2,4-triazol-1-ylmethyl) - sik Ropentanoru, 4- (2,2-difluoro - 1,3-benzodioxol-4-yl) pyrrole-3-carbonitrile, at least one selected from
And as an active ingredient,
A seed disinfectant used for at least one disease selected from the group consisting of rice sapling seedling disease, rice blast disease and rice sesame leaf blight . The seed disinfectant according to claim 1, wherein the silver ion content in the silver-supporting zeolite (a) is 0.1 to 15.0% by weight. The seed disinfectant according to any one of claims 1 to 2, wherein the content of the component (b) is 1 to 2000 parts by weight with respect to 100 parts by weight of the silver-supporting zeolite (a).