JP4922550B2 - Process for producing banaba leaf extract with high corosolic acid content - Google Patents

Process for producing banaba leaf extract with high corosolic acid content Download PDF

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JP4922550B2
JP4922550B2 JP2004075394A JP2004075394A JP4922550B2 JP 4922550 B2 JP4922550 B2 JP 4922550B2 JP 2004075394 A JP2004075394 A JP 2004075394A JP 2004075394 A JP2004075394 A JP 2004075394A JP 4922550 B2 JP4922550 B2 JP 4922550B2
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corosolic acid
extract
banaba
activated carbon
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欣也 高垣
千尋 森
宜仁 木村
尚明 吉田
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Toyo Shinyaku Co Ltd
Tokiwa Phytochemical Co Ltd
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Tokiwa Phytochemical Co Ltd
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Description

本発明は、バナバ葉を60容量%〜100容量%のエタノールを含有する水溶液で抽出し、得られた抽出物をバナバ葉の乾燥質量100質量部に対し10〜20質量%の活性炭で処理して抽出物中のコロソリン酸含有量を高めた後に、濃縮して得られた濃縮液中の沈殿物を分離し、沈殿物を回収する工程を包含する、コロソリン酸を高含有するバナバ葉抽出物の製造方法に関する。   In the present invention, banaba leaves are extracted with an aqueous solution containing 60% by volume to 100% by volume of ethanol, and the obtained extract is treated with 10 to 20% by mass of activated carbon with respect to 100 parts by mass of the dry mass of banaba leaves. An extract of banaba leaves high in corosolic acid, comprising the steps of separating the precipitate in the concentrate obtained by concentrating after increasing the corosolic acid content in the extract, and collecting the precipitate It relates to the manufacturing method.

バナバは熱帯アジアに棲息するミソハギ科の植物であり、古くからこの葉を煎じて飲料として食されており、バナバ葉には糖尿病に対する効果があることから、民間薬として使われてきていた。近年においては、食生活が豊かになった反面、偏った栄養の取りすぎによって生ずる肥満や肥満によって引き起こされる糖尿病などの生活習慣病の発症率、または発症はしていないが、発症の危険性が高い、いわゆる予備群が増加していることから、食品として摂取することで肥満や糖尿病を予防する、いわゆる健康食品の原料として広く用いられるようになってきている(例えば特許文献1)。   Banaba is a plant belonging to the family Lamiaceae that lives in tropical Asia. It has been used as a beverage since decocting this leaf, and banaba leaves have been used as a folk medicine because they have an effect on diabetes. In recent years, while eating habits have become richer, the incidence of lifestyle-related diseases such as obesity caused by too much biased nutrition and diabetes caused by obesity has not occurred, or there is no risk of onset. Since the so-called reserve group is increasing, it has been widely used as a raw material for so-called health foods that prevent obesity and diabetes by ingestion as food (for example, Patent Document 1).

一方、バナバ葉については成分を含め様々な検討がなされており、この中でもバナバ葉に含まれるコロソリン酸は、体内のブドウ糖移動の賦活物質であることが明らかになり、糖尿病及びその発症に影響を与える血糖値を低下させることができ、注目を集めている。   On the other hand, various studies have been conducted on banaba leaves, including ingredients. Among them, corosolic acid contained in banaba leaves has been found to be an activator of glucose movement in the body, affecting diabetes and its onset. It can lower blood sugar levels, and has attracted attention.

このように、バナバ葉より機能的な成分を抽出して、原料とする試みがなされており、バナバ葉を熱水抽出した後にスチレン−ジビニルベンゼン系樹脂により機能成分を抽出する方法や、バナバ葉からの抽出条件を検討し、コロソリン酸含有量を高める方法等が開示されている(例えば特許文献2〜3)。
特開2001−103928号公報 特開平7−228539号公報 特開2000−169384号公報
In this way, attempts have been made to extract functional components from banaba leaves and use them as raw materials. After extracting banaba leaves with hot water, a method of extracting functional components with styrene-divinylbenzene-based resin, or banaba leaves The method for increasing the corosolic acid content by examining the extraction conditions from the potato is disclosed (for example, Patent Documents 2 to 3).
JP 2001-103928 A JP-A-7-228539 JP 2000-169384 A

しかし、バナバ葉から得られる抽出物、特にコロソリン酸を高含有するバナバ葉を得ることは難しく、原料の違いや抽出条件の違いで、十分な含有量を持つバナバ葉抽出物が得られないといった問題点があった。   However, it is difficult to obtain an extract obtained from banaba leaves, in particular, banaba leaves containing a high amount of corosolic acid, and a banaba leaf extract having a sufficient content cannot be obtained due to differences in raw materials or extraction conditions. There was a problem.

そこで本発明者等は、バナバ葉からコロソリン酸を高含有する抽出物を得る方法について鋭意検討を重ねた結果、バナバ葉をエタノールを含有する水溶液で抽出し、この抽出物を活性炭処理し、さらに濃縮して沈殿物を回収することで、コロソリン酸含有量の高いバナバ葉抽出物を得られることを見出し、本発明に至った。   Therefore, as a result of intensive studies on a method for obtaining an extract containing corosolic acid in a high amount from banaba leaves, the present inventors extracted banaba leaves with an aqueous solution containing ethanol, treated the extract with activated carbon, and By concentrating and collecting the precipitate, it was found that a banaba leaf extract having a high corosolic acid content can be obtained, and the present invention has been achieved.

すなわち本発明は、バナバ葉を60容量%〜100容量%のエタノールを含有する水溶液で抽出して粗抽出液を得る工程;得られた粗抽出液をバナバ葉の乾燥質量100質量部に対し10〜20質量%の活性炭で処理し、コロソリン酸含有量を高めた処理液を得る工程;濃縮して得られた濃縮液中の沈殿物を分離し、沈殿物を回収する工程を包含する、乾燥質量換算で9質量%以上のコロソリン酸を含有するバナバ葉抽出物の製造方法に関する。   That is, the present invention is a step of extracting a banaba leaf with an aqueous solution containing 60% by volume to 100% by volume of ethanol to obtain a crude extract; the obtained crude extract is 10 parts per 100 parts by mass of the banaba leaf dry mass. A step of obtaining a treatment liquid having a corosolic acid content increased by treatment with ˜20% by mass of activated carbon; a step comprising separating the precipitate in the concentrated liquid obtained by concentration and collecting the precipitate; The present invention relates to a method for producing a banaba leaf extract containing 9% by mass or more of corosolic acid in terms of mass.

本発明によれば、バナバ葉を60容量%〜100容量%のエタノールを含有する水溶液で抽出し、さらに、原料の乾燥質量に対し10〜20質量%の活性炭処理することによって、乾燥質量換算で9質量%以上のコロソリン酸を含有するバナバ葉抽出物を得ることができる。   According to the present invention, banaba leaves are extracted with an aqueous solution containing 60% by volume to 100% by volume of ethanol, and further treated with 10 to 20% by mass of activated carbon with respect to the dry mass of the raw material. A banaba leaf extract containing 9% by mass or more of corosolic acid can be obtained.

以下本発明について説明する。なお、以下に説明する構成は、本発明を限定するものではなく、発明の趣旨の範囲で種々改変することができるものである。   The present invention will be described below. Note that the configuration described below does not limit the present invention and can be variously modified within the scope of the gist of the invention.

本発明の組成物の原料として使用されるバナバ葉は、フィリピンなどで産出するバナバ(Lagerstroemia Speciosa、 Linn. またはPers.)の生葉を乾燥して用いる。バナバ葉は腐食しやすいため、収穫した後に直ちに乾燥させて、乾燥物とするのが好ましい。生葉の乾燥は自然乾燥または風乾のいずれであってもよいが、好ましくは風乾により、強制的に乾燥させることが好ましい。乾燥は、水分含量が20質量%以下、好ましくは10質量%以下となるように行うことで、微生物の腐食を防止し、バナバ葉中の成分を安定に保持させることができる。   The banaba leaves used as the raw material of the composition of the present invention are dried banaba (Lagerstroemia Speciosa, Linn. Or Pers.) Fresh leaves produced in the Philippines and the like. Since banaba leaves are susceptible to corrosion, it is preferable to dry them immediately after harvesting to obtain dried products. Fresh leaves may be dried either naturally or air-dried, but preferably forcedly dried by air-drying. Drying is performed so that the moisture content is 20% by mass or less, preferably 10% by mass or less, thereby preventing the corrosion of microorganisms and keeping the components in the banaba leaf stably.

この乾燥物は、以下に述べる工程、(a)エタノール水溶液による抽出工程、(b)活性炭による処理工程、(c)濃縮して沈殿物を回収する工程を行うことで、コロソリン酸を高含有するバナバ葉抽出物を得ることができる。   This dried product contains corosolic acid at a high content by performing the steps described below, (a) an extraction step with an aqueous ethanol solution, (b) a treatment step with activated carbon, and (c) a step of concentrating and recovering the precipitate. Banaba leaf extract can be obtained.

(a)エタノール水溶液による抽出工程
まず、乾燥物をエタノール水溶液で抽出を行い、粗抽出液を得る。抽出には、乾燥物をそのまま抽出してもよいが粉砕または細断して抽出することで効率よく抽出することができる。乾燥物の粉砕には、カッター、スライサー、ダイサーなどの当業者に公知の任意の機械または道具により粉砕する。粉砕した乾燥物の粒径は20mm以下、好ましくは10mm以下、最も好ましくは1mm以下である。乾燥物からのアルコール水溶液による抽出は、アルコールの含有量が60〜100容量%となるように調製し、乾燥物へ添加する。アルコール含有量が60容量%より低いと、乾燥物からコロソリン酸が溶出しにくくなり、結果として得られる粗抽出物中のコロソリン酸含有量が低下するためである。抽出のためにエタノール水溶液を添加する量または抽出の温度及び抽出の方法は特に制限されるわけではないが、乾燥物から溶液中にコロソリン酸が溶出されるように調製する。乾燥物へ添加するエタノール水溶液の量は、好ましくは乾燥物1質量部に対し15質量部以上である。15質量部より少ないと、乾燥物から効率よくコロソリン酸を溶出することができない。抽出の温度は、エタノール水溶液が沸騰する温度以下で、好ましくは30℃〜80℃、より好ましくは、50℃〜80℃で加熱し、必要に応じて加熱還流とすることでエタノールの蒸発を防ぎながら抽出を行うことができる。また、攪拌を行うことで抽出の効率を高めることができ、コロソリン酸を高含有する抽出液を得ることができる。また、複数回にわたって抽出することで乾燥物中のコロソリン酸をさらに効率よく抽出できる。また、後に述べる活性炭による処理の前に抽出液中の不溶物を除去してもよい。
(A) Extraction step with aqueous ethanol solution First, the dried product is extracted with an aqueous ethanol solution to obtain a crude extract. For extraction, the dried product may be extracted as it is, but can be extracted efficiently by pulverizing or chopping. The dried product is pulverized by any machine or tool known to those skilled in the art, such as a cutter, a slicer, and a dicer. The particle size of the pulverized dry product is 20 mm or less, preferably 10 mm or less, and most preferably 1 mm or less. Extraction from the dried product with an aqueous alcohol solution is carried out so that the alcohol content is 60 to 100% by volume and added to the dried product. When the alcohol content is lower than 60% by volume, corosolic acid is hardly eluted from the dried product, and the corosolic acid content in the resulting crude extract is reduced. The amount of ethanol aqueous solution added for extraction or the temperature and method of extraction are not particularly limited, but it is prepared so that corosolic acid is eluted from the dried product into the solution. The amount of the aqueous ethanol solution added to the dried product is preferably 15 parts by mass or more with respect to 1 part by mass of the dried product. If it is less than 15 parts by mass, corosolic acid cannot be efficiently eluted from the dried product. The extraction temperature is below the temperature at which the aqueous ethanol solution boils, preferably 30 ° C. to 80 ° C., more preferably 50 ° C. to 80 ° C., and heating to reflux as necessary to prevent ethanol evaporation. Extraction can be performed. Moreover, the efficiency of extraction can be improved by performing stirring, and an extract containing a high amount of corosolic acid can be obtained. In addition, corosolic acid in the dried product can be extracted more efficiently by extracting multiple times. Moreover, you may remove the insoluble matter in an extract before the process by activated carbon described later.

(b)活性炭による処理工程
次いで、得られた粗抽出液を活性炭へ接触させ、夾雑物を除去することによってコロソリン酸を高含有した処理液を得る。通常、活性炭は脱色等を目的として使用されるが、本発明においては、処理液の固形分中のコロソリン酸含有量を高める目的で使用する。活性炭は、好ましくは表面積が200〜800m/g、より好ましくは400〜800m/gのものを使用することで、抽出液中の夾雑物を効率よく吸着させることができ、結果として溶液中のコロソリン酸含有量が高められる。この範囲の表面積を有する活性炭であれば、各種の市販品を使用することができ、具体的には、カヤマックス(日本化薬株式会社)、活性炭カルボラフィン(武田薬品工業株式会社)などを挙げることができる。活性炭の量は、抽出に用いたバナバ葉の乾燥質量で100質量部あたり10〜20質量%を添加する。活性炭の量が多いほど粗抽出液中の成分が多く吸着されるため、コロソリン酸も活性炭に吸着され、含有量が低下すると考えられるが、通常脱色に使用する量、例えば5〜10質量%よりも多い10〜20質量%の活性炭を用いることで、コロソリン酸の収量がよく、得られた処理液の固形分中のコロソリン酸含有量を高めることができる。なお、10質量%よりも少ないと、夾雑物の除去には不十分で、コロソリン酸の含有量を高めることが難しくなる。
(B) Treatment step with activated carbon Next, the obtained crude extract is brought into contact with activated carbon, and impurities are removed to obtain a treatment solution containing corosolic acid in a high amount. Normally, activated carbon is used for the purpose of decolorization or the like, but in the present invention, it is used for the purpose of increasing the content of corosolic acid in the solid content of the treatment liquid. Activated carbon preferably has a surface area of 200 to 800 m 2 / g, more preferably 400 to 800 m 2 / g, so that impurities in the extract can be adsorbed efficiently. Corosolic acid content of is increased. As long as the activated carbon has a surface area in this range, various commercially available products can be used. Specifically, Kayamax (Nippon Kayaku Co., Ltd.), activated carbon carborafin (Takeda Pharmaceutical Co., Ltd.) and the like can be mentioned. be able to. The amount of the activated carbon is 10 to 20% by mass per 100 parts by mass based on the dry mass of the banaba leaf used for extraction. The larger the amount of activated carbon, the more components in the crude extract are adsorbed, so corosolic acid is also adsorbed on the activated carbon, and the content is considered to decrease, but the amount usually used for decolorization, for example, from 5 to 10% by mass By using a large amount of activated carbon of 10 to 20% by mass, the yield of corosolic acid is good, and the content of corosolic acid in the solid content of the obtained treatment liquid can be increased. In addition, when less than 10 mass%, it is inadequate for the removal of a foreign material, and it will become difficult to raise content of corosolic acid.

活性炭と抽出液の接触の方法は、当業者が通常用いる方法、例えばカラム法やバッチ法等で行うことが可能である。なお、活性炭で処理する場合は、処理時の温度をエタノールが沸騰する温度以下、好ましくは50℃〜80℃で処理を行うことで、抽出液に含まれる固形分中のコロソリン酸含有量を高めることができ、特に、加熱還流することが好ましい。   The contact method between the activated carbon and the extract can be performed by a method commonly used by those skilled in the art, such as a column method or a batch method. In addition, when processing with activated carbon, the corosolinic acid content in solid content contained in an extract is raised by processing at the temperature at the time of processing below the temperature which ethanol boils, Preferably it is 50 to 80 degreeC. In particular, heating to reflux is preferred.

カラム法を用いて処理するには、例えば、まず、活性炭をカラムに充填し、そのカラムに抽出液を通液し、溶出してきた液を回収する。さらに必要に応じて充填した活性炭の体積に対し、1〜10倍量の抽出時に用いたものと同じ濃度のエタノール水溶液を通液させ、好ましくはこの操作を繰り返し、還流させる。これにより、多くの夾雑物は活性炭に吸着され、コロソリン酸はカラムから十分溶出されるので、得られた溶液の固形分中のコロソリン酸含有量を高くすることができる。   In order to perform the treatment using the column method, for example, first, activated carbon is packed in a column, the extract is passed through the column, and the eluted liquid is collected. Further, if necessary, an ethanol aqueous solution having the same concentration as that used for the extraction of 1 to 10 times the volume of the activated carbon is passed through, and this operation is preferably repeated and refluxed. Thereby, many impurities are adsorbed on the activated carbon and corosolic acid is sufficiently eluted from the column, so that the content of corosolic acid in the solid content of the obtained solution can be increased.

バッチ法を用いて処理するには、粗抽出液に活性炭に加え、攪拌または還流しながら30分〜1時間接触させた後に、濾過または遠心分離により液層を回収する。さらに、コロソリン酸の収率を挙げるために、残渣を抽出時に用いたものと同じ濃度のエタノール水溶液で洗浄し、洗浄液を回収することもできる。   In order to process using the batch method, the crude extract is added to activated carbon and brought into contact with stirring or refluxing for 30 minutes to 1 hour, and then the liquid layer is recovered by filtration or centrifugation. Furthermore, in order to increase the yield of corosolic acid, the residue can be washed with an aqueous ethanol solution having the same concentration as that used during extraction, and the washing solution can be recovered.

(c)濃縮して沈殿物を回収する工程
次いで得られた処理液を濃縮し、濃縮して得られた濃縮液中の沈殿物を分離し、沈殿物を回収する。濃縮の方法は、加熱乾燥や減圧濃縮等、当業者が通常用いる方法によって行うことができるが、好ましくは減圧濃縮により行うことで処理液中の成分の変性を抑えることができる。濃縮は、濃縮液中のエタノール濃度が60容量%以下、好ましくは0〜60容量%となるまで濃縮する。得られた濃縮液には、エタノールの含有容量が低下することによって、コロソリン酸が優先的に沈殿した沈殿物が生じるため、沈殿物に含まれるコロソリン酸含有量は飛躍的に向上する。また、沈殿物を十分生じさせるために、1℃〜50℃で1時間〜24時間、静置してもよい。この沈殿物を濃縮液から遠心分離や濾過等の当業者が通常用いる方法で分離し、沈殿物を回収することでコロソリン酸を高含有するバナバ葉抽出物を得ることができる。なお、濃縮工程にて得られた沈殿物、すなわちバナバ葉抽出物を60容量%以下、好ましくは30容量%以下のエタノール水溶液で洗浄し、夾雑物を除去することで、バナバ葉抽出物中のコロソリン酸含有量を高めることもできる。
(C) Step of concentrating to recover the precipitate Next, the obtained treatment liquid is concentrated, the precipitate in the concentrated liquid obtained by concentration is separated, and the precipitate is recovered. The concentration method can be carried out by methods commonly used by those skilled in the art, such as heat drying and vacuum concentration. Preferably, the concentration of the components in the treatment liquid can be suppressed by concentration under reduced pressure. Concentration is performed until the ethanol concentration in the concentrated solution is 60% by volume or less, preferably 0 to 60% by volume. In the obtained concentrated solution, a precipitate in which corosolic acid is preferentially precipitated is generated due to a decrease in the ethanol content, so that the corosolic acid content contained in the precipitate is dramatically improved. Moreover, in order to fully produce a deposit, you may stand still at 1-50 degreeC for 1 hour-24 hours. The precipitate is separated from the concentrated solution by a method commonly used by those skilled in the art such as centrifugation and filtration, and the precipitate is recovered to obtain a banaba leaf extract containing a high amount of corosolic acid. In addition, the precipitate obtained in the concentration step, that is, the banaba leaf extract is washed with an ethanol aqueous solution of 60% by volume or less, preferably 30% by volume or less, to remove impurities, thereby removing the banaba leaf extract. Corosolic acid content can also be increased.

このようにして得られたバナバ葉抽出物は、このまま飲料等に使用することもできるが、エタノールが含有されているため、好ましくは乾燥し、粉末化する。乾燥には、熱風乾燥、高圧蒸気乾燥、減圧濃縮乾固、電磁波乾燥、凍結乾燥、噴霧乾燥などの当業者が通常用いる任意の方法により行われるが、好ましくは減圧濃縮乾固または凍結乾燥及び噴霧乾燥である。乾燥は、固形分中の水分含量が10質量%以下、好ましくは5質量%以下となるまで乾燥することが好ましい。噴霧乾燥を行う場合は、必要に応じてデキストリン、シクロデキストリン、デンプン、マルトースのような賦形剤を添加して行われる。好適にはデキストリンが用いられ、抽出物とデキストリンとの比は、乾燥物質量比で1:5〜10:1が好ましい。   The banaba leaf extract thus obtained can be used as it is in beverages and the like, but is preferably dried and powdered because it contains ethanol. The drying is performed by any method commonly used by those skilled in the art, such as hot air drying, high pressure steam drying, vacuum concentration drying, electromagnetic wave drying, freeze drying, spray drying, etc., but preferably vacuum concentration drying or freeze drying and spraying. It is dry. The drying is preferably performed until the water content in the solid content is 10% by mass or less, preferably 5% by mass or less. When spray drying is performed, an excipient such as dextrin, cyclodextrin, starch or maltose is added as necessary. Preferably, dextrin is used, and the ratio of extract to dextrin is preferably 1: 5 to 10: 1 in terms of dry substance amount.

なお、乾燥して得られた抽出物の乾燥物は、固まりを形成することが多いため、乾燥後に粉砕したバナバ葉抽出粉末とすることが好ましい。粉末化することで、様々な形態に応用がしやすくなるだけでなく、食品として摂取した場合においては、吸収の効率が高くなり、優れた血糖値上昇抑制効果を得られやすくなる。   In addition, since the dried product of the extract obtained by drying often forms a lump, it is preferable to use a banaba leaf extract powder pulverized after drying. By powdering, not only can it be easily applied to various forms, but when it is ingested as a food, the absorption efficiency is increased, and an excellent blood glucose level increase suppressing effect can be easily obtained.

粉砕は、上記にて述べたバナバ葉の粉砕方法と同様の方法を採用することが可能である。   The pulverization can employ the same method as the banaba leaf pulverization method described above.

粉砕によって得られる粉末は、好ましくは1000μm以下、好ましくは500μm以下、より好ましくは20〜200μmとすることで、血糖値上昇抑制効果等の高い生理活性を得ることが可能である。   The powder obtained by pulverization is preferably 1000 μm or less, preferably 500 μm or less, and more preferably 20 to 200 μm, whereby high physiological activity such as an effect of suppressing an increase in blood glucose level can be obtained.

このようにして得られたバナバ葉抽出物は、コロソリン酸を高含有し、抽出物の乾燥質量でコロソリン酸を9質量%以上、好ましくは9質量%〜50質量%含有し得る。また、所定量の活性炭で処理することによって、バナバ葉の独特な風味が除去され、さらには、バナバ葉中の好ましくない成分も除去されていると考えられ、安全性が高く、かつ汎用的な食品原料として使用しやすい抽出物となる。   The banaba leaf extract thus obtained contains a high content of corosolic acid and can contain 9 mass% or more, preferably 9 mass% to 50 mass% of corosolic acid in terms of the dry mass of the extract. Moreover, by treating with a predetermined amount of activated carbon, the unique flavor of banaba leaf is removed, and further, it is considered that undesirable components in banaba leaf are also removed, which is highly safe and versatile. The extract is easy to use as a food ingredient.

本発明によって得られた、バナバ葉抽出物及びこの粉末は、そのまま用いられるか、あるいは賦形剤、増量剤、結合剤、増粘剤、乳化剤、着色料、香料、食品添加物、調味料などと混合され得る。例えば、ローヤルゼリー、プロポリス、ビタミン類(A、B群、C、D、E、K、葉酸、パントテン酸、ビオチン、及びこれらの誘導体等)ミネラル(鉄、マグネシウム、カルシウム、亜鉛等)、セレン、キチン・キトサン、レシチン、ポリフェノール(カテキン類、アントシアニン類、プロアントシアニジンなどの縮合型タンニン、ガロタンニン等の加水分解型タンニン、フラボノイド類及びこれらの誘導体等)、カロテノイド(リコピン、アスタキサンチン、ゼアキサンチン、ルテイン等)、サポニン(イソフラボン、ジンセサノイド、グリチルリチン酸等)、キサンチン誘導体(カフェイン等)、脂肪酸、アミノ酸、タンパク質(コラーゲン、エラスチン等)、ムコ多糖類(ヒアルロン酸、コンドロイチン、デルマタン、ヘパラン、ヘパリン、ケタラン、ならびにこれらの塩等)、アミノ糖(グルコサミン、アセチルグルコサミン、ガラクトサミン、アセチルガラクトサミン、ノイラミン酸、アセチルノイラミン酸、ヘキソサミン、およびそれらの塩等)、食物繊維(難消化性デキストリン、アルギン酸、グアガム、ペクチン、グルコマンナン等)、オリゴ糖(イソマルトオリゴ糖、環状オリゴ糖等)リン脂質及びその誘導体(フォスファチジルコリン、スフィンゴミエリン、セラミド等)、含硫化合物(アリイン、セパエン、タウリン、グルタチオン、メチルスルホニルメタン等)、糖アルコール、キノン類(コエンザイムQ10等)、リグナン類(セサミン等)及びこれらを含有する動植物抽出物、ウコン、ショウガ等の根菜類、麦若葉末等のイネ科植物やケール等のアブラナ科植物の緑葉等、他の食品素材と組み合わせられる。そしてさらに、ハードカプセル、ソフトカプセルなどのカプセル剤、錠剤、丸剤に成型されるか、または粉末、顆粒、ティーバッグなどの形態にする。これらは、その形状または好みに応じて、そのままか、あるいは水、湯、もしくは牛乳などに溶いて、または成分を浸出して飲むことができる。   The banaba leaf extract and this powder obtained by the present invention are used as they are, or excipients, extenders, binders, thickeners, emulsifiers, coloring agents, flavorings, food additives, seasonings, etc. Can be mixed with. For example, royal jelly, propolis, vitamins (A, B group, C, D, E, K, folic acid, pantothenic acid, biotin, and derivatives thereof) minerals (iron, magnesium, calcium, zinc, etc.), selenium, chitin Chitosan, lecithin, polyphenol (condensed tannins such as catechins, anthocyanins, proanthocyanidins, hydrolyzed tannins such as gallotannins, flavonoids and derivatives thereof), carotenoids (lycopene, astaxanthin, zeaxanthin, lutein, etc.), Saponins (isoflavones, ginsaneoids, glycyrrhizic acid, etc.), xanthine derivatives (caffeine, etc.), fatty acids, amino acids, proteins (collagen, elastin, etc.), mucopolysaccharides (hyaluronic acid, chondroitin, dermatan, heparan, heparan, Phosphorus, ketalan, and salts thereof), amino sugars (glucosamine, acetylglucosamine, galactosamine, acetylgalactosamine, neuraminic acid, acetylneuraminic acid, hexosamine, and salts thereof), dietary fiber (indigestible dextrin, alginic acid) , Guar gum, pectin, glucomannan, etc.), oligosaccharides (isomalto-oligosaccharides, cyclic oligosaccharides, etc.), phospholipids and derivatives thereof (phosphatidylcholine, sphingomyelin, ceramide, etc.), sulfur-containing compounds (alliin, sepaene, taurine, Glutathione, methylsulfonylmethane, etc.), sugar alcohol, quinones (coenzyme Q10, etc.), lignans (sesamin, etc.) and animal and plant extracts containing these, root vegetables such as turmeric, ginger, etc. And kale Green leaves, etc. of the Rana family plant, is combined with other food materials. Further, it is molded into capsules such as hard capsules and soft capsules, tablets and pills, or in the form of powders, granules, tea bags and the like. These can be taken as is or dissolved in water, hot water, milk or the like, or the components can be leached depending on the shape or preference.

また、本発明の抽出物または抽出物の粉末は、食品だけでなく、医薬品、医薬部外品、化粧品、トイレタリー用品などに広く適用し得る。例えば、化粧水、化粧クリーム、乳液、パック、ヘアトニック、シャンプー、ヘアリンス、トリートメント、ボディーシャンプー、洗顔剤、石鹸、ファンデーション、口紅、育毛剤、軟膏、入浴剤、歯磨剤、マウスウォッシュ、シップ、ゲルなどが挙げられ、当業者が用いることのできる基材や動植物抽出物を用いることができる。   Moreover, the extract or powder of the extract of the present invention can be widely applied not only to foods but also to pharmaceuticals, quasi drugs, cosmetics, toiletries and the like. For example, lotion, cosmetic cream, emulsion, pack, hair tonic, shampoo, hair rinse, treatment, body shampoo, facial cleanser, soap, foundation, lipstick, hair restorer, ointment, bath preparation, dentifrice, mouthwash, ship, gel The base material and animal and plant extract which can be used by those skilled in the art can be used.

本発明の抽出物の食品や化粧品などの外用剤への添加量は特に制限はないが、食品としては、好ましくは0.001〜100質量%、より好ましくは0.01質量%〜70質量%含有される。また、外用剤としては、好ましくは外用剤中に0.00001質量%〜50質量%、好ましくは0.0001質量%〜30質量%含有させれば、抽出物の持つ生理作用を得ることが可能である。   The amount of the extract of the present invention added to external preparations such as foods and cosmetics is not particularly limited, but as a food, it is preferably 0.001 to 100% by mass, more preferably 0.01% to 70% by mass. Contained. In addition, as an external preparation, the physiological action of the extract can be obtained by adding 0.00001% by mass to 50% by mass, preferably 0.0001% by mass to 30% by mass in the external preparation. It is.

以下、本発明をより詳細に説明するが、この範囲に限定されるものではない。   Hereinafter, the present invention will be described in more detail, but the present invention is not limited to this range.

(実施例1)
バナバ葉1kgへ70Lの85容量%エタノールを加え、80℃にて1時間加熱還流し、減圧下で濾過し抽出液を得た。さらに残渣に70Lの85容量%エタノールを加え、80℃にて1時間加熱還流し、減圧下で濾過し、この濾液を抽出液と合わせ、粗抽出液を得た。この粗抽出液に、活性炭カルボラフィン(武田薬品工業株式会社製)を100g添加し、1時間80℃にて還流後、減圧下にてろ過を行い、脱色処理液を得た。この脱色処理液を60℃減圧下にて10Lまで濃縮したところで濃縮を止め、20℃にて一晩放置したところ、沈殿物と上澄み液とに分離した。この液を、遠心分離機を用いて沈殿を回収し、得られた沈殿物を、再度30容量%エタノール5L中にて室温にて撹拌洗浄し、減圧下にてろ過し沈殿物を得た。この沈殿物を、60℃減圧下にて一晩乾燥したところ、乾燥物として15gを得た。得られた黄色の乾燥物中のコロソリン酸含量を、高速液体クロマトグラフィーを用いて測定したところ、コロソリン酸含量は9.8%であった。

Example 1
70 L of 85 vol% ethanol was added to 1 kg of banaba leaf, heated to reflux at 80 ° C. for 1 hour, and filtered under reduced pressure to obtain an extract. Further, 70 L of 85% by volume ethanol was added to the residue, heated to reflux at 80 ° C. for 1 hour, filtered under reduced pressure, and the filtrate was combined with the extract to obtain a crude extract. 100 g of activated carbon carborafine (manufactured by Takeda Pharmaceutical Company Limited) was added to the crude extract, and after refluxing at 80 ° C. for 1 hour, filtration was performed under reduced pressure to obtain a decolorization treatment solution. When this decolorization treatment liquid was concentrated to 10 L under reduced pressure at 60 ° C., the concentration was stopped and left at 20 ° C. overnight, whereupon it separated into a precipitate and a supernatant. A precipitate was collected from this liquid using a centrifuge, and the obtained precipitate was again washed with stirring in 5 L of 30 vol% ethanol at room temperature, and filtered under reduced pressure to obtain a precipitate. This precipitate was dried overnight at 60 ° C. under reduced pressure to obtain 15 g as a dried product. When the corosolic acid content in the obtained yellow dried product was measured using high performance liquid chromatography, the corosolic acid content was 9.8%.

Claims (1)

バナバ葉を60容量%〜100容量%のエタノールを含有する水溶液で抽出して粗抽出液を得る工程;得られた粗抽出液をバナバ葉の乾燥質量100質量部に対し10〜20質量%の活性炭で処理し、コロソリン酸含有量を高めた処理液を得る工程;濃縮して得られた濃縮液中の沈殿物を分離し、30容量%以下のエタノール水溶液で洗浄した沈殿物を回収する工程を包含する、乾燥質量換算で9質量%以上のコロソリン酸を含有するバナバ葉抽出物の製造方法。 Extracting banaba leaves with an aqueous solution containing 60% to 100% by volume of ethanol to obtain a crude extract; the obtained crude extract is 10-20% by mass with respect to 100 parts by mass of the dry mass of banaba leaves. A step of obtaining a treatment liquid treated with activated carbon to increase the content of corosolic acid; a step of separating the precipitate in the concentrated liquid obtained by concentration and collecting the precipitate washed with an aqueous ethanol solution of 30% by volume or less The manufacturing method of the banaba leaf extract containing 9 mass% or more corosolic acid in conversion of the dry mass which contains.
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