JP4904086B2 - Jellyfish decomposition waste liquid treatment apparatus and treatment method, and microorganism - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an apparatus and a method of efficiently treating a salt-containing waste liquid such as a decomposition waste liquid of a jellyfish to decompose and dispose of at a low cost without using a special apparatus and causing environmental pollution, and a microorganism used for at least one of the treating apparatus and the treating method. <P>SOLUTION: The apparatus for treating the salt-containing waste liquid has a support onto which the microorganism capable of growing in the salt-containing liquid is fixed and a contact means for bringing the salt-containing waste liquid into contact with the support. In the method of treating the salt-containing waste liquid, the salt-containing waste liquid is brought into contact with the support onto which the microorganism capable of growing in the salt-containing liquid is fixed. The microorganism growing in the salt-containing waste liquid is used at least for one of the treating apparatus or the treating method. <P>COPYRIGHT: (C)2007,JPO&amp;INPIT

Description

  INDUSTRIAL APPLICABILITY The present invention is used in at least one of a processing apparatus and a processing method that makes it possible to dispose of a salt-containing waste liquid such as an organic substance decomposition processing liquid such as jellyfish without polluting the environment. Related to microorganisms.

  Jellyfish floating in seawater, etc. occur mainly during warm periods and cause various damages. For example, Echizen jellyfish, which occur in large quantities in the seas near Japan, appear on the coasts of various regions along the ocean current, and in recent years have caused tremendous damage to fisheries such as coastal net fishing. Also, in coastal plant facilities such as power plants and steelworks that require a large amount of cooling water, seawater intake is provided to use seawater as cooling water. Damage due to blockage, that is, damage that water intake is restricted or stopped and hinders its operation has become a problem. Furthermore, the jellyfish mainly composed of the Echizen jellyfish landed in the fishing net or the jellyfish landed at the seaside plant facility, once landed, becomes industrial waste and cannot be discharged into the ocean or discarded as it is. There is a problem.

  Therefore, conventionally, various methods for discarding jellyfish once landed have been studied (see Patent Documents 1 to 9). In any treatment method, organic waste liquid containing salt is inevitably discharged. Particularly, jellyfish is an organic waste liquid containing a large amount of salt because 95% or more of the body components are moisture. The jellyfish decomposition waste liquid mainly contains proteins, lipids, free amino acids, polysaccharides, minerals, etc. In other words, BOD components (biochemical oxygen demand), COD (chemical oxygen demand) Amount) component, phosphorus, and nitrogen are contained in large amounts, and a high concentration of 3% by mass or more is also included. If such jellyfish decomposition waste liquid is discharged as it is, rivers, oceans, lakes, groundwater, etc. will be contaminated, resulting in environmental pollution due to eutrophication and salt damage. Therefore, it is desired to provide a processing apparatus and a processing method that can be disposed of without waste.

  However, the salt-containing waste liquid such as the jellyfish decomposition waste liquid corrodes the processing apparatus when performing mechanical disposal due to the salt contained therein, and cannot be efficiently decomposed and discarded. On the other hand, when performing biological treatment using enzymes or microorganisms, although efficient decomposition and disposal treatment is possible compared to the mechanical waste treatment, sufficient enzyme activity is not obtained, There are problems such as the death of microorganisms. For this reason, conventionally, the decomposition waste liquid of the jellyfish has been once diluted with fresh water and then decomposed and discarded using activated sludge or the like. However, in this case, a large amount of fresh water for dilution is required, a dilution step is required, which is not efficient, and special equipment such as an activated sludge treatment facility is required, which is not convenient. . Accordingly, there is still provided a processing apparatus and a processing method that can dispose of salt-containing waste liquids such as jellyfish decomposition waste liquids at low cost efficiently and without requiring special equipment and without causing environmental pollution. The current situation is not.

JP 2001-300505 A JP 2000-5738 A Japanese Patent Laid-Open No. 11-244833 JP 2000-145196 A JP 2001-198566 A JP 2003-53303 A JP-A-11-179327 JP 2001-95564 A JP 2002-136852 A

  An object of the present invention is to solve the conventional problems and achieve the following objects. That is, the present invention provides a processing apparatus and a processing method for disposing of salt-containing waste liquids such as jellyfish decomposition waste liquids at low cost and efficiently without requiring special equipment and without causing environmental pollution. Another object of the present invention is to provide a microorganism that can be used in at least one of the processing apparatus and the processing method.

In order to solve the above-mentioned problems, the present inventor has made extensive investigations, and as a result, a salt-containing waste liquid such as a jellyfish decomposition waste liquid is brought into contact with a carrier on which microorganisms that can grow in the salt-containing liquid are immobilized. By processing, it has been found that it can be disassembled and disposed of efficiently and inexpensively without requiring special equipment, and without causing environmental pollution. Means for solving the problems are as follows.
<1> An apparatus for treating a salt-containing waste liquid, comprising: a carrier on which microorganisms capable of growing in a salt-containing liquid are immobilized; and a contact means for bringing the salt-containing waste liquid into contact with the carrier. In the apparatus for treating a salt-containing waste liquid according to <1>, the salt-containing waste liquid is brought into contact with the carrier on which the microorganism is fixed by the contact means. At this time, the salt-containing waste liquid is efficiently decomposed to a level at which organic substances such as proteins contained therein can be disposed of by the action of the microorganism. As a result, the salt-containing waste liquid treatment apparatus functions as a so-called bioreactor. According to the salt-containing waste liquid treatment apparatus, the salt-containing waste liquid containing proteins such as jellyfish can be efficiently produced at low cost. It does not require special equipment and can be disassembled and discarded without causing environmental pollution. The treatment apparatus for the salt-containing waste liquid, like a general bioreactor, can (1) be capable of continuous treatment, (2) comprehensively immobilize microorganisms and enzymes, and increase the treatment capacity per unit volume. (3) It is possible to perform high-speed processing, (4) the apparatus can be made compact, and (5) microorganisms according to the purpose of wastewater treatment such as nitrifying bacteria can be fixed.
<2> The apparatus for treating a salt-containing waste liquid according to <1>, wherein the carrier on which microorganisms that can grow in the salt-containing liquid are fixed is formed of any one of a polysaccharide, a protein, a synthetic polymer, and an inorganic substance. is there. In the apparatus for treating a salt-containing waste liquid according to <2>, since the carrier is formed of a polysaccharide or the like, the microorganism is easily and efficiently fixed and easily detached from the carrier. There is nothing to do. Therefore, the salt-containing waste liquid can be decomposed only by bringing the salt-containing waste liquid to be treated into contact with the carrier.
<3> The apparatus for treating a salt-containing waste liquid according to any one of <1> to <2>, wherein the carrier on which microorganisms that can grow in the salt-containing liquid are fixed has a porous structure. In the apparatus for processing a salt-containing waste liquid according to <3>, since the carrier has a porous structure, the salt-containing waste liquid to be processed can pass through the carrier and is fixed in the carrier. In addition, since the contact area with the microorganism is large, the salt-containing waste liquid is efficiently decomposed.
<4> The apparatus for treating a salt-containing waste liquid according to any one of <1> to <3>, wherein the microorganism that can grow in the salt-containing liquid includes a salt-resistant organic matter waste liquid-using microorganism group in sludge. In the apparatus for treating a salt-containing waste liquid according to <4>, the salt-containing waste liquid is efficiently decomposed by the action of the microorganism group using the salt-resistant organic waste liquid.
<5> The salt-containing waste liquid according to any one of <1> to <4>, wherein the microorganism that can grow in the salt-containing liquid is fixed to the carrier by at least one of an attachment method, a crosslinking method, and a comprehensive method It is a processing device. In the apparatus for treating a salt-containing waste liquid according to <5>, since the microorganism is fixed to the carrier by an adhesion method or the like, the microorganism is not easily detached from the carrier, and the The salt-containing waste liquid is decomposed.
<6> The above <1> to <5, wherein a carrier on which microorganisms that can grow in a salt-containing liquid are fixed is contained in a container, and the contact means is a liquid-feeding device that feeds the salt-containing waste liquid into the container. > Is a treatment apparatus for salt-containing waste liquid according to any of the above. In the apparatus for treating a salt-containing waste liquid according to <6>, the container functions as a reaction tank of a bioreactor, and the salt-containing waste liquid is contained in the container containing the carrier by the liquid feeding unit. The liquid is sent. As a result, the salt-containing waste liquid is decomposed discontinuously or continuously.
<7> The apparatus for processing a salt-containing waste liquid according to <6>, wherein the liquid-feeding device includes a pipe that guides the salt-containing waste liquid and a liquid-feeding pump that feeds the salt-containing waste liquid toward the carrier. is there. In the salt-containing waste liquid treatment apparatus according to <7>, the salt-containing waste liquid is fed through the pipe by the action of the liquid feed pump.
<8> The apparatus for treating a salt-containing waste liquid according to any one of <6> to <7>, wherein the container includes a dissolved oxygen concentration adjusting unit that adjusts a dissolved oxygen concentration in a salt-containing waste liquid contained therein. It is. In the salt-containing waste liquid treatment apparatus according to <8>, the dissolved oxygen concentration in the salt-containing waste liquid stored in the container is controlled to be constant by the dissolved oxygen concentration adjusting means. For this reason, the decomposition activity of the salt-containing waste liquid by the microorganisms fixed to the carrier is always kept stable and constant. As a result, the decomposition treatment efficiency of the salt-containing waste liquid is excellent.
<9> The apparatus for treating a salt-containing waste liquid according to any one of <1> to <8>, further including a sterilization unit that sterilizes the salt-containing waste liquid brought into contact with the carrier by the contact unit. In the salt-containing waste liquid treatment apparatus according to <9>, microorganisms and the like detached from the carrier are effectively sterilized by the sterilizing means in the salt-containing waste liquid to be discarded.
<10> The apparatus for treating a salt-containing waste liquid according to any one of <1> to <9>, further including a desalting means for performing a desalination treatment on the salt-containing waste liquid brought into contact with the carrier by the contact means. is there.
<11> The salt content according to any one of <9> to <10>, wherein at least one of the sterilizing means and the desalting means is a voltage application device that applies a voltage to the salt-containing waste liquid discharged from the container. This is a waste liquid treatment device. In the apparatus for treating a salt-containing waste liquid according to <11>, a voltage is applied to the salt-containing waste liquid to be disposed of by the voltage application device, so that microorganisms and the like contained in the salt-containing waste liquid can be effectively used. It is sterilized and the salt-containing waste liquid is effectively desalted. As a result, for example, the salt-containing waste liquid to be discarded can be used as a fertilizer or the like.
<12> The apparatus for treating a salt-containing waste liquid according to any one of <1> to <11>, wherein the salt-containing waste liquid is an organic waste liquid. In the salt-containing waste liquid treatment apparatus according to <12>, since the salt-containing waste liquid is an organic waste liquid, if the salt-containing waste liquid is disposed of as it is, it causes environmental pollution. Since the organic matter is decomposed and disposed of, there is no risk of environmental pollution.
<13> The apparatus for treating a salt-containing waste liquid according to <12>, wherein the organic waste liquid is a jellyfish decomposition liquid. In the apparatus for treating a salt-containing waste liquid according to <13>, since the organic waste liquid is the jellyfish decomposition solution, the organic waste liquid contains an organic substance and a salt, but the efficiency of the microorganisms immobilized on the carrier is improved. Thus, the organic matter is decomposed and removed.
<14> The apparatus for treating a salt-containing waste liquid according to any one of <1> to <13>, wherein a salt concentration in the salt-containing waste liquid is 1% by mass or more. In the apparatus for treating a salt-containing waste liquid according to <14>, the salt concentration in the salt-containing waste liquid is relatively high, but due to the action of the microorganisms immobilized on the simple substance, the salt efficiently Organic substances contained in the salt-containing waste liquid are decomposed.
<15> A method for treating a salt-containing waste liquid, comprising contacting a salt-containing waste liquid with a carrier on which microorganisms that can grow in the salt-containing liquid are immobilized. In the salt-containing waste liquid treatment method according to <15>, the salt-containing waste liquid is brought into contact with a carrier on which the microorganism is immobilized. At this time, organic substances and the like contained in the salt-containing waste liquid are efficiently decomposed and removed by the action of the microorganisms. For this reason, according to the method for treating a salt-containing waste liquid, the salt-containing waste liquid containing proteins such as jellyfish can be decomposed at low cost efficiently without requiring special equipment and without causing environmental pollution. Can be discarded.
<16> The apparatus for treating a salt-containing waste liquid according to any one of <1> to <14>, which is capable of growing in a salt-containing liquid, and the method for treating a salt-containing waste liquid according to <15>. It is a microorganism characterized by being used in at least one of them.
<17> The microorganism according to <16>, which includes a microorganism group using salt-resistant organic matter waste liquid in sludge.
<18> The microorganism according to any one of <16> to <17>, comprising yeast.
<19> The microorganism according to <18>, further including a bacterial group.
<20> The microorganism according to any one of <18> to <19>, wherein the yeast is Rhodotorula mucilaginosa.
<21> The microorganism according to any one of <18> to <20>, wherein the yeast is Rhodotorula mucilaginosa 126-Age strain (Accession No. FERM P-20896).
<22> The group of bacteria comprises bacteria belonging to the genus Sphingobacteria, bacteria belonging to the genus Pseudomonas, bacteria belonging to the genus Flavobacterium, and bacteria belonging to the genus Bacillus The microorganism according to any one of <19> to <21>, which is a bacterial group consisting of at least one species selected from the group.
<23> The aforementioned <22> wherein the bacterium belonging to the genus Sphingobacterium is at least any one selected from Sphingobacterium multivolum and Sphingobacterium spiritiborum <22 >.
<24> The microorganism according to any one of <22> to <23>, wherein the bacterium belonging to the genus Bacillus is Bacillus pumilus.
<25> The microorganism according to any one of <16> to <24>, which is obtained by accumulating a group of microorganisms using salt-resistant organic waste liquid in sludge in a salt-containing waste liquid to be treated.

  According to the present invention, conventional problems can be solved, and salt-containing waste liquids such as jellyfish decomposition waste liquid can be decomposed efficiently at low cost without requiring special equipment and causing environmental pollution. It is possible to provide a processing apparatus and a processing method that can be disposed of, and a microorganism that can be used in at least one of the processing apparatus and the processing method.

(Salt-containing waste liquid treatment equipment, treatment method, and microorganism)
The apparatus for treating a salt-containing waste liquid according to the present invention comprises a carrier on which microorganisms that can grow in the salt-containing liquid are immobilized, and contact means for bringing the salt-containing waste liquid into contact with the carrier. It has other means suitably selected, for example, sterilization means.
The method for treating a salt-containing waste liquid according to the present invention includes a contact step of bringing a salt-containing waste liquid into contact with a carrier on which microorganisms that can grow in the salt-containing liquid are immobilized, and other treatments appropriately selected as necessary. For example, a sterilization process is included. The salt-containing waste liquid treatment method can be preferably carried out using the salt-containing waste liquid treatment apparatus of the present invention. That is, the contact step can be suitably performed by the contact unit, the sterilization step can be performed by the sterilization unit, and the other processing can be performed by the other unit.
Hereinafter, although the processing apparatus of the salt containing waste liquid of this invention is demonstrated, the processing method of the salt containing waste liquid of this invention is demonstrated through the description.
Further, the microorganism of the present invention is used in at least one of the processing apparatus and the processing method. The microorganisms of the present invention will also be described through the following description of the apparatus for treating a salt-containing waste liquid of the present invention.

<Microorganism>
The microorganism is not particularly limited as long as it can grow in the salt-containing liquid, and can be appropriately selected according to the purpose. For example, it preferably includes a salt-resistant organic waste liquid-utilizing microorganism group. When the microorganism contains the group of microorganisms using the salt-resistant organic waste liquid, it is advantageous in that it is excellent in decomposition efficiency of the salt-containing waste liquid and can be subjected to continuous treatment, repeated treatment, and the like.

There is no restriction | limiting in particular as the said salt-resistant organic substance waste liquid utilization microorganism group, Although it can select suitably according to the objective, For example, the salt tolerance organic substance waste liquid utilization microorganism group in sludge can be used.
There is no restriction | limiting in particular as said sludge, Although it can select suitably according to the objective, For example, a seabed sludge is preferable, Furthermore, a layer (for example, about 5-20 cm from the seabed surface, for example, preferably 5 Submarine sludge collected from about 10 cm) is more preferable. Submarine sludge collected from a layer close to the seafloor surface is advantageous in that COD and the like can be more efficiently reduced under aerobic conditions.

Further, the microorganism is particularly preferably a microorganism obtained by accumulating and culturing a group of microorganisms using salt-resistant organic waste liquid in the sludge in a salt-containing waste liquid to be treated. As such microorganisms, for example, organic waste liquid after decomposing jellyfish is mixed with sludge, preferably with the above-mentioned seabed sludge, and mixed with nutrient salts such as sugars and phosphates as necessary. And a microorganism group obtained by acclimatizing a microorganism group that propagates using the organic waste liquid as a main nutrient source (a jellyfish waste liquid utilizing microorganism group). Such a microorganism group can be actively propagated using the salt-containing waste liquid such as a jellyfish decomposition solution to be treated as a main nutrient source, and can effectively remove COD components and the like in the organic waste liquid. This is advantageous.
The microorganism group using the salt-resistant organic waste liquid is selected from microorganism groups having a higher ability to decompose and remove COD components and the like by repeating the acclimatization sequentially using the salt-containing waste liquid such as a jellyfish decomposition solution. Can be integrated.

  The type of the microorganism is not particularly limited as long as the organic matter contained in the salt-containing waste liquid can be decomposed. For example, yeast, bacteria (bacteria), mold, filamentous fungi such as actinomycetes, protozoa, Etc. These may be used individually by 1 type and may use 2 or more types together. The microorganism may be, for example, an aerobic bacterium or an anaerobic bacterium, but in general, an aerobic bacterium is preferably exemplified.

Moreover, it is particularly preferable that the microorganism contains yeast.
By using at least yeast as the microorganism, the amount of sludge generated during waste liquid treatment can be reduced more efficiently, COD and the like can be reduced more efficiently, and more This is advantageous in that it can be made compact.

There is no restriction | limiting in particular as said yeast, Although it can select suitably according to the objective, For example, Rhodotorula mucilaginosa (Rhodotorula mucilainosa) etc. are mentioned suitably. The Rhodotorula musillaguinosa is not particularly limited and may be appropriately selected depending on the intended purpose. The 126-Aege strain deposited under the number FERM P-20896 can be suitably used. The various properties of the 126-Age strain are as shown in the examples described later, for example.
Use of Rhodotorula musillaguinosa as the yeast is advantageous in that it is suitable for use in combination with other microorganisms such as bacteria (bacteria), and COD can be more efficiently reduced.
In addition, the said yeast can also be obtained by isolate | separating from the salt-resistant organic substance waste liquid utilization microbe group in the said sludge, for example.

  In addition, the yeast is preferably used alone, but it is also preferable to use it together with other microorganisms as described above, for example. For example, bacteria (bacteria group) can be used in combination with the yeast. By using the yeast and bacteria (bacteria group) together, there is an advantage that the pigment produced by the yeast can be suppressed and the clarification of the waste liquid can be promoted.

The bacterium (bacteria) is not particularly limited and may be appropriately selected depending on the intended purpose. Examples thereof include bacteria belonging to the genus Sphingobacteria, bacteria belonging to the genus Pseudomonas, flavobacteria Examples include bacteria belonging to the genus (Flavobaterium), bacteria belonging to the genus Bacillus, and the like.
The bacterium belonging to the genus Sphingobacterium is not particularly limited and may be appropriately selected depending on the intended purpose. Etc.
The bacterium belonging to the genus Bacillus is not particularly limited and may be appropriately selected depending on the intended purpose. Examples thereof include Bacillus pumilus.

  In addition, there is no restriction | limiting in particular as each usage-amount ratio in the case of using together the said yeast and other microorganisms, such as bacteria (bacteria group), According to the objective, it can select suitably.

<Carrier>
There is no restriction | limiting in particular as a support | carrier which fixes the said microorganisms, According to the kind, purpose, etc. of the said microorganisms, the shape, a structure, a magnitude | size, a material, etc. can be selected suitably.
Examples of the shape of the carrier include a spherical shape, a granular shape, a lump shape (pellet shape), a sheet shape, a column shape, a net shape, and a capsule shape. These may be used individually by 1 type and may use 2 or more types together.
As the structure of the carrier, it may be formed of one kind of member, may be formed of two or more members, may be a single layer structure, or is a laminated structure. Also good. These may be used individually by 1 type and may use 2 or more types together.
Further, the fine structure of the carrier is not particularly limited as long as the microorganism immobilized on the carrier can be brought into contact with the salt-containing waste liquid. preferable. When the carrier has these structures, the contact area between the microorganisms immobilized on the carrier and the salt-containing waste liquid can be increased, which is advantageous in that the decomposition treatment efficiency of the salt-containing waste liquid is excellent. .
The size of the carrier can be appropriately selected according to the size of a container or the like that contains the carrier, the type of the microorganism, and the like. The size of the carrier may be uniform (constant) or different from each other.

Suitable examples of the material for the carrier include polysaccharides, proteins, synthetic polymers, and inorganic substances. These may be used individually by 1 type and may use 2 or more types together.
Examples of the polysaccharide include cellulose, dextran, agarose, sodium alginate, agar, carrageenan and the like. Among these, various microorganisms can be maintained at a high concentration and excellent in the permeability of components in the waste liquid. Agar is preferred in that the granulation operation is easy, the toxicity is low, and the treatment and disposal are easy.
As the protein, for example, inactivated one is preferable, and among them, gelatin, albumin, collagen and the like can be mentioned.
Examples of the synthetic polymer include acrylamide, polyvinyl alcohol, polyethylene glycol, sodium polyacrylate, polyvinyl chloride, polystyrene, polyurethane, and a photocurable resin.
Examples of the inorganic material include silica gel, activated carbon, sand, zeolite, porous glass, anthracite, zeolite, foam brick, molten slag, and among these, silica gel and activated carbon which are porous materials are more preferable. preferable.

The method for immobilizing the microorganism on the carrier is not particularly limited and can be carried out according to a known method and can be appropriately selected according to the purpose. For example, an adhesion method (carrier binding method), cross-linking, A law, a comprehensive method, etc. are mentioned suitably. These may be used alone or in combination of two or more.
The adhesion method (carrier binding method) is a method of immobilizing the microorganism on the surface of the carrier that is insoluble in water. The crosslinking method is a method of crosslinking with a reagent having two or more functional groups. The enveloping method is a method of encapsulating the microorganism in a gel lattice (lattice type) or coating with a polymer film (microcapsule).

  In addition, there is no restriction | limiting in particular as a fixing position of this microorganism at the time of fix | immobilizing the said microorganisms to the said support | carrier, Although it can select suitably according to the objective, etc., such as aerobic and anaerobic property of the said microorganisms. For example, when the microorganism is aerobic, the microorganism is preferably immobilized in the vicinity of the surface of the carrier. When the microorganism is anaerobic, the microorganism is contained inside the carrier. It is preferable that it is fixed to.

The case of producing a gel-like carrier using agar as the immobilization carrier will be described below.
The agar preferably granulates the carrier at a concentration of 3% by mass, which is a concentration of 3% by mass, for example, with little leakage of immobilized microorganisms or penetration into the interior, excellent durability, long life, and high stability. .
Water is mixed so that the agar becomes 3% by mass, and the agar is dissolved while stirring at 60 ° C. or higher. Thereafter, in order to stably immobilize the microorganism group having no heat resistance, it is preferable to mix with the microorganism at as low a temperature as possible (for example, 55 ° C. or less) as long as it does not solidify.
As the microorganism, for example, a microorganism collected by an operation such as centrifugation can be used.
The agar mixed with the microorganisms is cooled to room temperature and solidified, and then placed in a molding container and cut into a desired shape to obtain the microorganism-immobilized carrier.

<Container>
The carrier on which the microorganisms are immobilized (hereinafter sometimes referred to as “microorganism-immobilized carrier”) is preferably contained in a container when brought into contact with the salt-containing waste liquid. It is preferable that the carrier is contained in the container in that the contact between the carrier and the salt-containing waste liquid can be performed efficiently and controlled.
There is no restriction | limiting in particular as said container, The shape, a structure, a magnitude | size, a material, etc. can be suitably selected according to the objective.
Suitable examples of the shape of the container include a cylindrical shape.
Further, the material of the container is preferably formed of a salt-resistant material, more preferably formed of glass, resin, stainless steel, etc. Among these, the inside of the container is visible. Those are preferred.
There is no restriction | limiting in particular as a filling rate of the said microorganisms fixed support | carrier in the said container, According to the objective, it can select suitably, 100% may be sufficient, and less than 100% may be sufficient.
A plurality of the containers may be connected in parallel or in series depending on the load of the salt-containing waste liquid.

-Means for adjusting dissolved oxygen concentration-
The container is preferably provided with a dissolved oxygen concentration adjusting means for adjusting the dissolved oxygen concentration in the salt-containing waste liquid accommodated therein. In this case, it is advantageous in that the growth conditions of the microorganisms immobilized on the carrier accommodated in the container can always be maintained at suitable conditions, and in particular, due to a change in dissolved oxygen concentration, the salt-containing waste liquid This is advantageous in that fluctuations in decomposition activity and the like can be effectively suppressed.

  Examples of the dissolved oxygen concentration adjusting means include means capable of sending air into the container in the form of bubbles, air lift means, stirring means, and the like. The dissolved oxygen adjusting means has a control mechanism capable of increasing or decreasing the dissolved oxygen concentration in the container based on measurement information from an oxygen concentration sensor or the like provided in the container. Is preferred. In this case, there is no restriction | limiting in particular as said oxygen concentration sensor, According to the objective, it can select suitably, For example, a commercial item etc. can be used conveniently. There is no restriction | limiting in particular as said control mechanism, According to the objective, it can select suitably, For example, a feedback control mechanism, a PID control mechanism, a fuzzy control mechanism, etc. are mentioned.

  The dissolved oxygen concentration adjusting means is preferably controlled so that the dissolved oxygen concentration in the salt-containing waste liquid is preferably 2 mg / L or more, more preferably 4 mg / L or more in terms of DO value.

-Contact means-
The contact means is not particularly limited as long as the salt-containing waste liquid can be brought into contact with the carrier, and can be appropriately selected depending on the purpose. For example, the salt-containing waste liquid is fed into the container. Preferred examples include a liquid feeding device.
The liquid feeding device is not particularly limited and may be appropriately selected depending on the intended purpose. For example, a pipe for feeding the salt-containing waste liquid and a liquid feeding liquid for feeding the salt-containing waste liquid toward the carrier The thing etc. which have a pump are mentioned. In addition, there is no restriction | limiting in particular about the shape of a pipe | tube, a structure, a magnitude | size, a material, etc., According to the objective, it can select suitably. You may design the drive of the said liquid feeding pump so that control is possible by control means, such as a computer.

-Other means-
There is no restriction | limiting in particular as said other means, According to the objective, it can select suitably, For example, a disinfection means, a desalting means, a temperature control means, an illumination means, a coagulation precipitation means, a filtration means etc. are mentioned.

--Sterilization means--
As the sterilizing means, for example, the salt-containing waste liquid that is brought into contact with the carrier by the contact means and decomposed can be sterilized. For example, a voltage is applied to the salt-containing waste liquid. Examples thereof include a voltage application device to be applied, an ozone contact device for contacting ozone, and an ultraviolet irradiation device for irradiating ultraviolet rays. These may be used individually by 1 type and may use 2 or more types together.

--Desalting means--
As the desalting means, for example, the salt-containing waste liquid that is brought into contact with the carrier by the contact means and decomposed can be subjected to a desalting treatment. For example, for the salt-containing waste liquid, Examples include a voltage applying device that applies a voltage. In addition, when the processing apparatus of the said salt containing waste liquid is equipped with the voltage application apparatus as said sterilization means, desalting can be achieved with this voltage application apparatus.

--- Temperature adjusting means--
As the temperature means, it is sufficient that the salt-containing waste liquid brought into contact with the microorganism-immobilized carrier by the contact means can be adjusted to a temperature that does not inhibit the growth and decomposition reaction of the microorganism, for example, a thermostat, What combined suitably a heater, an air blower, etc. are mentioned.

--Lighting means--
By providing the illumination means, it is possible to increase the growth of aerobic microorganisms that exhibit a light reaction among the microorganisms, and to decompose the salt-containing waste liquid more effectively.
There is no restriction | limiting in particular as said illumination means, According to the objective, it can select suitably, An incandescent lamp, a fluorescent lamp, etc. are mentioned. Moreover, it may be a daylighting means for outdoor light.

-Means for aggregation and precipitation-
As the coagulation sedimentation means, for example, the salt-containing waste liquid is brought into contact with the microorganism-immobilized carrier by the contact means, and after the treatment, the generated suspended substance can be aggregated and precipitated. If it is, there will be no restriction | limiting in particular, According to the objective, it can select suitably, For example, what utilized reagents, such as calcium hydroxide, calcium oxide, sodium aluminate, etc. are mentioned.

--- Filtration means--
The filtration means is not particularly limited as long as it can remove the precipitate formed by the coagulation sedimentation means, and can be appropriately selected according to the purpose. For example, a column using activated carbon, etc. Is mentioned.

<Salt-containing waste liquid>
In the apparatus and method for treating a salt-containing waste liquid according to the present invention, the salt-containing waste liquid to be decomposed contains salt and contains organic substances to the extent that it cannot be disposed of as it is. If it is, there is no restriction | limiting in particular, Although it can select suitably according to the objective, For example, organic substance waste liquid etc. are mentioned.
The organic waste liquid is preferably in a mode in which the microorganisms immobilized on the carrier are easily decomposed. For example, in the case of a protein, it is preferably in a state of being cleaved to amino acids by a proteolytic enzyme or the like. .
In the process of generating the organic waste liquid, when the salinity concentration decreases, the decomposition efficiency by the microorganisms can be maintained, for example, by adding new salinity.
In addition, when the organic matter concentration in the organic waste liquid is low, one or more of saccharides such as glucose, nitrogen sources such as ammonium sulfate and ammonium chloride, and inorganic salts such as potassium chloride and sodium phosphate are appropriately added to the organic matter. Mixing with the waste liquid can contribute to the active growth of the microorganisms immobilized on the carrier.

-Jellyfish decomposition solution-
There are various kinds of the organic waste liquid, and any of these may be used. In the present invention, a jellyfish decomposition liquid is particularly preferable. In the jellyfish decomposition solution, there are organic molecules at the amino acid to peptide level, and it is extremely difficult in terms of technology and cost to further decompose the organic molecules that have been reduced to this level. According to the present invention, it is advantageous in that an organic substance contained in such a jellyfish decomposition solution can be effectively decomposed.

  The salt concentration in the salt-containing waste liquid is not particularly limited and may be appropriately selected depending on the purpose.For example, it is 1% by mass or more, and without adversely affecting the decomposition activity of the microorganism, From the viewpoint of efficiently decomposing organic matter in a salt-containing waste liquid such as a jellyfish decomposition solution, the concentration is preferably about seawater.

  The jellyfish decomposition solution is not particularly limited and may be appropriately selected according to the purpose. However, a solution obtained by decomposing jellyfish using a prionase enzyme developed by the present inventors is preferable, and A liquid obtained by decomposing jellyfish using a jellyfish decomposing apparatus developed by the present inventors is more preferable.

--Prionase enzyme--
The prionase enzyme is an enzyme developed by the present inventor and degrades abnormal prion proteins that cause prion diseases such as bovine spongiform encephalopathy (mad cow disease), scrapie (sheep), Creutzfeldt-Jakob disease, etc. It is an enzyme obtained from the enzyme prionase-producing microorganism. In general, the abnormal prion protein is very stable against various proteolytic enzymes, disinfectants and autoclave treatments, and treatment methods are limited. According to the prionase enzyme, the abnormal prion protein is The jellyfish collected in Tokyo Bay can be completely decomposed within 30 minutes under optimum conditions.

  The microorganisms that produce the prionase enzyme are microorganisms belonging to Streptomyces actinomycetes, which are known to produce a large amount of physiologically active substances such as antibiotics, anticancer agents, and immunosuppressants, and are not pathogenic. In general, a large number of actinomycetes are present in soil and are responsible for organic matter decomposition in the environment. The prionase enzyme exhibits optimal proteolytic activity on the alkali side at 70 ° C. The prionase enzyme can also decompose other types of jellyfish such as jellyfish jellyfish, bizen jellyfish, red jellyfish and scorpion jellyfish. The jellyfish decomposition solution after the decomposition of jellyfish by the prionase enzyme has a very low viscosity, and the molecules are cleaved from the amino acid constituting the protein to the size of the peptide.

As the Streptomyces genus actinomycetes, for example, those selected from sp.
As the SP species actinomycetes, for example, those having the following A to C, preferably A to D bacteriological properties are suitably exemplified, such as 99-GP-2D-5 strain (FERM P-19336) and More preferred are those selected from the derived strains.
A. Morphological properties (1) A relatively long wavy aerial hyphae is elongated from a branched basic hyphae and rarely has a hook or loop shape.
(2) The mature spore chain links 10 to 50 oval spore, and the size of the spore is about 0.6 to 0.7 × 0.8 to 1.0 micron.
(3) The spore surface is smooth.
(4) No roticular branch, mycelial bundle, spore and motility spore are observed.
B. The cell wall composition contains LL-type 2,6-diaminopimelic acid.
C. 90% or more, preferably 95% or more homology to Streptomyces actinomycetes due to a partial base sequence (400 to 500 bp) of 16S ribosomal RNA gene.
D. Growth state in various media (1) On the growth of yeast / malt agar medium (ISP-medium 2, cultured at 27 ° C.) and light yellow [2ea, LtWheat], grayish white [1 dc, Putty] to bright olive ash [ 1 1/2 ge, Lt Olive Gray] slightly aerial mycelia were formed, and no soluble pigment was observed.
(2) Oatmeal agar medium (ISP-medium 3, 27 ° C. culture), colorless to light yellow [1 1/2 ca, Cream], white aerial mycelia grow slightly, soluble pigment is observed I can't.
(3) On a starch / inorganic salt agar medium (ISP-medium 4, 27 ° C. culture) and colorless growth, white aerial mycelia are slightly formed and no soluble pigment is observed.
(4) Glycerin / asparagine agar medium (ISP-medium 5, 27 ° C. culture), growth is colorless, aerial hyphae do not grow, and no soluble pigment is observed.
(5) On a sucrose / nitrate agar medium (27 ° C. culture), colorless growth, white aerial mycelia are slightly formed and no soluble pigment is observed.

  Examples of the derived strain of Streptomyces sp. 99-GP-2D-5 (FERM P-19336) include progeny of Streptomyces sp. 99-GP-2D-5 and Streptomyces sp. 99-GP-2D. -5 strains which are artificial or natural mutants and have a protease-producing ability capable of degrading a hardly degradable protein. The Streptomyces sp. 99-GP-2D-5 strain, like other Streptomyces strains, is susceptible to changes in its properties, for example, by artificial mutation means using ultraviolet rays, X-rays, drugs, and the like. Can be mutated.

--- Jellyfish decomposition device-
The jellyfish decomposition device is a jellyfish decomposition device that uses a siphon. (1) No agitation device is required in the decomposition tank. (2) Since one pump can be operated, the running cost is reduced and maintenance management is performed. (3) The decomposition liquid does not overflow from the decomposition tank, (4) The state of decomposition can be easily observed, (5) The apparatus can be easily enlarged, and (6) Continuous It can be decomposed automatically.

  As a specific example of the jellyfish decomposition apparatus, a wet processing apparatus shown in FIG. 4 is preferably exemplified. In the apparatus shown in FIG. 4, a treatment liquid containing the prionase previously stored in the circulation / warming tank 50 (hereinafter, referred to as a “jellyfish decomposition composition”) is supplied to the gate valve 140 in the pipe as “ "Open", the gate valve 60, the gate valve 70, the gate valve 110 and the gate valve 130 are "closed", the liquid feed pump 4 provided in the pipe is driven to pump up, and transferred into the decomposition tank 10 . Then, the jellyfish decomposing composition flows into the decomposing tank 10 from the liquid flow inlet, contacts the jellyfish accommodated in the partition tank 20, and the jellyfish is contact-treated by the jellyfish decomposing composition. . At this time, the jellyfish is contact-treated so as to be pushed upward by the jellyfish decomposing composition flowing into the inside from the lower part of the decomposition tank 10. That is, the jellyfish is about to fall in the direction of gravity, whereas the jellyfish decomposing composition comes into contact with the jellyfish about to fall. For this reason, the contact treatment is effectively performed by the synergistic effect of the gravity applied to the jellyfish and the fluid pressure of the jellyfish decomposing composition, and the jellyfish is efficiently decomposed.

  A part of the jellyfish decomposition composition flowing into the decomposition tank 10 flows out into the siphon tube 30 from the liquid flow outlet provided in the lower part of the decomposition tank 10. Then, along with an increase in the liquid amount of the jellyfish decomposition composition flowing into the decomposition tank 10 (increase in the water level), it is connected to the liquid distribution outlet in the decomposition tank 10 and extends upward from the vicinity of the liquid distribution outlet. In the siphon tube 30 that is provided and curves downward near the upper part of the decomposition tank 10, the liquid amount of the jellyfish decomposition composition increases (the water level increases) at the same rate. When the liquid amount (water level) of the jellyfish decomposing composition in the siphon tube 30 reaches the curved portion of the siphon tube 30, the jellyfish decomposing composition in the decomposition tank 10 is liquid according to the principle of siphon. The material is continuously transferred from the distribution outlet to the outside through the siphon tube 30, and continuously transferred from the tip 170 of the siphon tube 30 into the circulation / heating tank 50. At this time, in the partition tank 20, the jellyfish falls by its own weight as the jellyfish decomposition composition is reduced, and when all of the jellyfish decomposition composition is transferred to the outside, the partition tank 20. It collides with the bottom surface of the plate and breaks into small pieces due to the impact and the like.

  The jellyfish decomposing composition transferred into the circulation / warming tank 50 is pumped up again by driving the liquid feed pump 40 provided in the pipe and transferred into the decomposition tank 10 again. Thereby, the second contact process is performed in the decomposition tank 10. At this time, the gate valve 140 in the piping is “open”, and the gate valve 60, gate valve 70, gate valve 110, and gate valve 130 are “closed”. In addition, if the liquid feeding pump 40 provided in the said piping is continuously driven, the re-transfer in the decomposition tank 10 and the recontact process in the decomposition tank 10 can be repeated continuously. By performing this contact treatment a plurality of times, the jellyfish accommodated in the partition tank 20 are completely decomposed and dissolved in the jellyfish decomposing composition.

  Further, in the circulation / heating tank 50, the jellyfish decomposition composition transferred by the siphon tube 30 is allowed to warm or react by passing through the heating tube 80 and circulating as necessary. it can. That is, the jellyfish decomposition composition is uniformly mixed by passing through the heating pipe 80 and circulating in the circulation / heating tank 50, and is also mixed into the jellyfish decomposition composition by the heater 90. By heating to the optimum temperature of the enzyme contained (when the temperature of the jellyfish decomposition composition has already reached the optimum temperature of the enzyme contained therein, it is necessary to operate the heater 90) The jellyfish degrading solution as a solution in which the jellyfish protein is uniformly dissolved as a result of complete and efficient degradation of undegraded jellyfish fragments (jellyfish protein) contained in the jellyfish decomposing composition A composition is prepared. In order to allow the jellyfish decomposition composition in the circulation / heating tank 50 to pass through the heating pipe 80 and circulate in the circulation / heating tank 50, the gate valve 70 in the piping is "open". The gate valve 60, the gate valve 130, and the gate valve 140 are set to “closed”, and the liquid feed pump 40 provided in the pipe is driven. At this time, all of the jellyfish decomposing composition in the circulation / heating tank 50 may be circulated in the circulation / heating tank 50 by passing through the heating tube 80, or the circulation / heating tank 50 may be circulated. Even if a part of the composition for decomposing jellyfish in the warm bath 50 is circulated in the circulation / heating bath 50 through the heating tube 80, the remaining portion is transferred into the decomposition bath 10. Good.

<Method of treating salt-containing waste liquid>
As a method for treating the salt-containing waste liquid, for example, as shown in the examples described later, the microorganisms show the best growth using the salt-containing waste liquid treatment apparatus of the present invention shown in FIG. The salt-containing waste liquid can be fed and circulated into the container provided with the microorganism-immobilized carrier that has been adjusted to a temperature capable of performing the process. By repeating the above steps as necessary, the organic matter in the salt-containing waste liquid is decomposed to a desired degree.

  The waste water from which the COD components and the like have been removed can be transferred to an electrolysis tank by, for example, operating a branch pipe for the purpose of sterilizing microorganisms leaking from the microorganism group-immobilized carrier. At this time, it is desirable that the transfer path of the waste liquid to the container filled with the immobilized microorganisms is closed by a branch pipe provided with a valve in order to prevent the microorganisms from being killed. As described above, the sterilization process can be repeated and returned to the container until the sterilization process is completed.

  Examples of the present invention will be described below, but the present invention is not limited to these examples.

(Example 1: Treatment of hot water treatment jellyfish waste liquid)
(1) Preparation of salt-containing waste liquid (organic jellyfish waste liquid) The jellyfish was heated with hot water at 90 ° C. or higher for about 1 hour, completely dissolved, and then cooled to room temperature to prepare a jellyfish waste liquid.

(2) Acclimatization of salt-resistant organic matter-utilizing microorganism group 100 mL of the jellyfish-derived salt-containing waste liquid obtained in (1) above was placed in a 500 mL Erlenmeyer flask, and 10 g of seabed mud was mixed. Furthermore, the salt-resistant organic matter-utilizing microorganism group contained in the seabed low mud was conditioned under the conditions of 27 ° C. and 180 rpm.

(3) Preparation of microorganisms 1 mL of the solution containing the salt-tolerant organic substance-utilizing microorganism group obtained in (2) above was mixed with the jellyfish organic waste liquid prepared in the same manner as in (1) above, and repeated acclimatization in the same manner. . By repeating the acclimatization several times, it is possible to accumulate a high concentration of microorganisms suitable for the treatment method of a highly active salt-containing waste liquid.

(4) Preparation of microorganism-immobilized carrier The microorganism group using the salt-tolerant organic waste liquid obtained in (3) was collected by centrifugation (10 minutes, 10,000 xg). After dissolving at 100 ° C, 3% agar (made by DIFCO) cooled to 50 ° C or less and microbial cells collected by centrifugation are mixed to a concentration of 1%, and a silicon tube (inner diameter 6 mm) Was injected with a syringe. Once the agar immobilized with the microorganisms injected into the silicon tube has solidified, it is extruded with a rod-shaped extruder thinner than the inner diameter of the silicon tube, and then the solidified agar is cut to a length of 3 mm to fix the microorganisms. A modified carrier was prepared.

(5) Treatment of salt-containing waste liquid Treatment was carried out using the apparatus shown in FIG. In FIG. 1, 1 is a wastewater tank, 2 is a transfer pump, 3 is a three-way cock, 4 is a venting device (dissolved oxygen concentration adjusting means), 5 is a bioreactor (container), 6 is an electrolysis tank (sterilizing means), 7 Indicates a gate valve.

  In the treatment process of the organic waste liquid, the organic waste liquid transferred from the waste liquid tank 1 by the transfer pump 2 at a flow rate of 150 mL per minute was operated so as to be transferred to the bioreactor 5 by the operation of the three-way cock. The bioreactor 5 was filled with the microorganism group-immobilized support prepared by the above methods (1) to (4), and air was supplied from the aeration apparatus so that the bioreactor was in an aerobic condition. The volume of the waste liquid tank 1 is 18 liters, and the volume of the bioreactor 5 is 1.8 liters. The waste liquid tank 1 passed through the bioreactor 5 and was circulated again to the waste liquid tank 1 to treat the organic waste liquid. The results are shown in FIG.

  From FIG. 2, the salt-containing waste liquid had a COD (chemical oxygen demand) value of 1,280 ppm before decomposition with the microorganism-immobilized support decreased to 122 ppm after 3 days, and further 88 ppm after 7 days. It turned out that it decreased to.

  After the treatment is finished, the waste liquid leaks from the bioreactor 5 into the organic waste liquid by passing through the electrolysis tank 6 and circulating the waste liquid tank 1 without passing through the bioreactor 5 by operating the three-way cock 3. The microbial population was sterilized. A platinum electrode was installed in the electrolysis tank, and the direct current of 9V and 9A was energized and circulated for 15 minutes to electrolyze the salt contained in the waste liquid as an electrolyte. The result of the bactericidal effect is shown in FIG.

From the results shown in FIG. 3, it was found that the microorganism group, which was 6.75 × 10 ⁷ per mL before electrolysis, was reduced to 1.14 × 10 ⁴ per mL by the electrolysis treatment for 15 minutes.

  The waste liquid treated water after the above steps can be discharged out of the system by releasing the gate valve 7. From the results of Example 1, it was confirmed that the COD component can be efficiently removed in a short time from the organic waste liquid containing salt by the treatment method according to the present invention.

(Example 2: Treatment of enzyme-treated jellyfish waste liquid)
In Example 1, except that the salt-containing waste liquid (organic jellyfish waste liquid), which is a decomposition target, was prepared by decomposing jellyfish by the following method using prionase as a degrading enzyme using the apparatus shown in FIG. The salt-containing waste liquid was treated in the same manner as in Example 1.

In the apparatus shown in FIG. 4, the treatment liquid containing prionase (composition for jellyfish decomposition) previously stored in the circulation / heating tank 50 is transferred into the decomposition tank 10 and stored in the partition tank 20. In contact with the jellyfish. The jellyfish is contact-treated so as to be pushed up by the jellyfish decomposition composition flowing into the interior from the lower part of the decomposition tank 10.
Part of the jellyfish decomposition composition that has flowed into the decomposition tank 10 flows out into the siphon tube 30 from the liquid flow outlet provided at the lower part of the decomposition tank 10. Then, along with an increase in the liquid amount of the jellyfish decomposition composition flowing into the decomposition tank 10 (increase in the water level), it is connected to the liquid distribution outlet in the decomposition tank 10 and extends upward from the vicinity of the liquid distribution outlet. In the siphon tube 30 that is provided and curves downward near the upper part of the decomposition tank 10, the liquid amount of the jellyfish decomposition composition increases (the water level increases) at the same rate. When the liquid amount (water level) of the jellyfish decomposing composition in the siphon tube 30 reaches the curved portion of the siphon tube 30, the jellyfish decomposing composition in the decomposition tank 10 is liquid according to the principle of siphon. The material is continuously transferred from the distribution outlet to the outside through the siphon tube 30, and continuously transferred from the tip 170 of the siphon tube 30 into the circulation / heating tank 50. At this time, in the partition tank 20, the jellyfish falls by its own weight as the jellyfish decomposition composition is reduced, and when all of the jellyfish decomposition composition is transferred to the outside, the partition tank 20. It collides with the bottom surface of the plate and breaks into small pieces due to the impact and the like.

  The jellyfish decomposing composition transferred into the circulation / warming tank 50 is pumped up again by driving the liquid feed pump 40 provided in the pipe and transferred into the decomposition tank 10 again. Thereby, the second contact process is performed in the decomposition tank 10. By performing this contact treatment a plurality of times, the jellyfish accommodated in the partition tank 20 are completely decomposed and dissolved in the jellyfish decomposition composition, thereby obtaining an organic waste liquid of jellyfish as the salt-containing waste liquid.

  In Example 2 in which the salt-containing waste liquid (jellyfish organic waste liquid) obtained by the above method was treated, the COD value decreased more rapidly than in Example 1, and it was found that the COD component could be removed efficiently.

(Example 3: Search for microorganisms suitable for treatment of waste liquid containing salt)
In Example 3, a search was made for microorganisms suitable for the treatment of the salt-containing waste liquid of the present invention. Specifically, a jellyfish waste liquid-use microorganism group that grows using the jellyfish waste liquid as a medium is searched as described in (1) to (2) below, and then the yeast and bacteria group in the obtained jellyfish waste liquid-use microorganism group Were identified as follows (3) to (4).

(1) Preparation of jellyfish waste liquid <Jellyfish sampling>
Moon jellyfish collected in Tokyo Bay were used. Jellyfish were collected using a net on board, and in order to prevent the jellyfish body component tissue from collapsing after thawing the jellyfish, the jellyfish was immersed in ethylene glycol, stored in a plastic bag, and stored frozen in dry ice. Thereafter, it was transported to the laboratory within 3 hours and stored at -60 ° C.

<Enzyme treatment of jellyfish>
The moon jellyfish in a plastic bag that had been stored frozen at −60 ° C. was thawed with running water. Next, a beaker was set on a hot stirrer, and 2 L of fresh water and a proteolytic enzyme (crude prionase) were mixed so as to be 0.1 to 0.2% in the beaker. Then, the jellyfish was decomposed | disassembled, always heating at 50 degreeC and putting the 1 mm mesh cage | basket in which the thawed moon jellyfish was put into a beaker, always stirring at low speed.
The jellyfish solution after decomposition (jellyfish waste solution) was transferred to a plastic tank and stored at 6 ° C.

<Ozone treatment of jellyfish waste liquid>
Using the ozone generator for the jellyfish waste liquid stored in the plastic tank for 3-4 months, the conditions of ozone generation amount (2g / hL), ozone concentration (0.5w%), jellyfish waste liquid temperature (10 ° C) Then, ozone treatment was performed for 4 hours. Thereafter, the jellyfish waste liquid was aerated under the conditions of maximum pressure (2.3 bar) and displacement (9.2 L / min) to remove residual ozone. In Example 3 and Examples 4 to 5 to be described later, the jellyfish was decomposed by prionase, and this ozone-treated waste liquid was used as a model of salt-containing waste liquid, unless otherwise specified.

(2) Search for microorganisms using jellyfish waste liquid 0.5 g of seabed sludge was placed in a 100 ml rotary flask. The submarine sludge is a Satake-type core sampler (separate company), which was collected by ship mainly from the coastal sea area in Tokyo Bay (for example, Shinonome Canal and Ariake in Koto-ku, Tokyo). Seabed sludge was collected in layers (core form) toward the seabed. For the purpose of activating the growth of microorganisms using jellyfish waste liquid, 0.1 g of glucose is mixed, and 50 ml of jellyfish waste liquid is further added, followed by shaking culture (180 rpm) at 27 ° C. in the seabed sludge. Accumulated culture of jellyfish waste liquid utilizing microorganisms was performed.
In order to search for microbial groups in the seabed sludge that proliferate in the jellyfish wastewater as a medium and efficiently reduce COD in the wastewater, the COD reduction rate and the depth of seabed sludge collection (depth from the seabed) ). The results are shown in FIG.
As a result, the highest COD reduction rate was 40.2% (4480 ppm → 2,680 ppm) in the sea mud collected at a depth of 5 cm from the seabed. Moreover, the COD reduction rate decreased as the core layer from which the sea mud was collected was deeper (FIG. 5).
From this result, it was recognized that more microorganisms that can reduce COD of the jellyfish waste liquid exist on the surface of the sea bottom than deep in the sea bottom. It is considered that the deeper the seabed, the more the sea mud is in an anaerobic state. In the culture under the aerobic condition set in this example, it is assumed that a high COD reduction rate was observed in the seafloor surface sample. Is done. From the above results, it was found that it is more desirable to collect microorganisms suitable for jellyfish waste liquid treatment from the surface of the seabed (for example, a depth of about 5 cm from the seabed).

(3) Identification of Yeast in Jellyfish Waste Liquid Utilizing Microorganism Group A plate of Yeast Malt Extract Agar (Becton Dickinson) medium (hereinafter sometimes referred to as YMA medium) is prepared, and shaking for 7 days in the above (2) As a result of culturing, the sample with the greatest COD reduction rate (sea mud collected at a depth of 5 cm from the seabed for 7 days in the jellyfish waste solution) was further accumulated in the jellyfish waste solution for 3 to 4 days. Using the obtained culture broth (containing a lot of jellyfish waste liquid-use microorganisms) as a specimen, streak culture was performed on a YMA plate medium with a flame-sterilized platinum loop. Two days later, the yeast colonies that appeared on the YMA plate medium were separated into a YMA slant medium under aseptic conditions and stored at 9 ° C. The slant-preserving yeast was subcultured about every other month.

-Observation of culture properties and morphology-
The preserved yeast was inoculated into a YMA plate medium, cultured at 27 ° C. for 3 days to 1 month, and colony properties were observed with the naked eye and a stereomicroscope SZH10. In addition, the microscopic characteristics of the specimen were observed by directly collecting bacterial cells from a plate to prepare a preparation and observing with a differential interference microscope BX50F4.

As a result of observing microscopic features, formation of vegetative cells having an oval shape to an elliptic shape was observed (FIG. 6). And it was confirmed that the vegetative growth was increased by budding. In addition, the formation of sexual reproductive organs was not observed on the plates cultured for 1 month.
Further, wet colonies were formed on the YM plate medium (FIGS. 7 and 8). The peripheral edge of each colony was smooth, and the color of the colony was reddish orange. Moreover, as a result of carrying out shaking culture (180 rpm, 27 degreeC) by putting an organic waste liquid in a rotary flask, the color tone of the organic waste liquid also changed from the cloudy color to the light red orange color (FIG. 9).

  As a result of morphological observation, the microbial cells separated from the specimen with the YMA medium were identified as yeast. Further, the morphological properties of the yeast are those of the known Rhodotorula mucilaginosa (for example, Barnett et al., 2000, Yeasts: Characteristics and Kent. 1998; see The Yeasts, a taxonomic study, 4th edition. Elsevier, Amsterdam, Netherlands).

-Physiological and biochemical property tests-
The physiological / biochemical property test of the yeast was conducted for each of the carbon source utilization test, saccharide fermentability test, nitrogen source utilization test, vitamin requirement test, temperature tolerance test, and drug resistance test. In addition, the culture | cultivation temperature of the said yeast other than a temperature tolerance test was performed at 25 degreeC.
The results of the physiological / biochemical property test are as shown in Tables 1 and 2 below.

* In Table 1, [+] indicates a positive reaction, [-] indicates a negative reaction, [w] indicates a weak positive reaction, and [D] indicates that a positive reaction was observed after 1 week or more from the start of the test.

* In Table 2, [+] indicates a positive reaction, [-] indicates a negative reaction, [w] indicates a weak positive reaction, and [D] indicates that a positive reaction was observed after 1 week or more from the start of the test.

  Physiological and biochemical properties of yeast isolated from the specimen in YMA medium are those of known Rhodotorula mucilaginosa (eg, Barnett et al., 2000; Yeasts: Characteristics and dentitives) .. University University Press, Cambridge, UK).

-28SrDNA-D1 / D2 region sequence analysis-
The preserved yeast was cultured at 25 ° C. for 7 days using a YMA plate medium, and genomic DNA was extracted from the collected colonies using DNeasy Plant Mini Kit (QIAGEN). Using this genomic DNA as a template, PCR amplification of the 18SrDNA 3′-end to 28SrDNA-D1 / D2 region DNA fragment was performed.
PCR is puReTaq Ready-To-Go PCR beads (Amersham Biosciences) and primer ITS5 (White et al., 1990; PCR protocols, a guide to methods and applications, Academic. And NL4 (see O'Donnell, 1993; The Fungal Homerph: Mitotic, Meiotic and Plemorphic Specification in Fungal Systemics, CAB International, Wallingford, p. stems Co., Ltd.) was carried out a thermal cycle on. The obtained DNA fragment was purified using QIAquick PCR Purification Kit (QIAGEN) and subjected to cycle sequencing reaction. The cycle sequencing reaction was performed on GeneAmp PCR System 9600 (Applied Biosystems) using ABI PRISM BigDye Terminator Kit (Applied Biosystems). For sequence analysis of the 28S rDNA-D1 / D2 region, primers NL1, NL2, NL3 and NL4 (O'Donnell, 1993; The Fungal Homeomorph: Mitotic, Miomatic and Plemorphic Specification in Bumping Instigation) .
[Primers used]
PCR primers;
ITS5 (Forward): 5′-GGAAGTAAAAGTCGTAACAAGG-3 ′ (SEQ ID NO: 1)
NL4 (Reverse): 5′-GGTCCGGTTTCAAGACGG-3 ′ (SEQ ID NO: 2)
Sequencing primer;
NL1 (Forward): 5′-GCATATCAATAAGCGGAGGAAAA-3 ′ (SEQ ID NO: 3)
NL2 (Reverse): 5′-CTCTCTTTTCAAAGTTCTTTCATCT-3 ′ (SEQ ID NO: 4)
NL3 (Forward): 5′-AGATGAAAAAGAACTTTGAAAAGAGAG-3 ′ (SEQ ID NO: 5)
NL4 (Reverse): 5′-GGTCCGTGTTTCAAGACGG-3 ′ (SEQ ID NO: 6)

The reaction product was purified using DyeEx ^™ 2.0 Spin Kit (QIAGEN), sequenced with ABI PRISM 3100 DNA Sequencer (Applied Biosystems), and AutoAssembler (Applied Biosystems Co.) The target nucleotide sequence was obtained.
The resulting sequence was as shown in Table 3 below (SEQ ID NO: 7).

In order to search a base sequence similar to the obtained sequence from the international DNA database (GenBank / EMBL / DDBJ), a homology search using BLAST (see, for example, Altschul et al., 1997; Nucleic Acids Research 25: 3389-3402) is performed. It was. An alignment is created using CLUSTAL W (for example, see Thompson et al., 1994; Nucleic Acids Research 22: 4673-4680) using the base sequences of the top 20 entries obtained as a result of searching the database, and MEGA ver3.1 (see, for example, Thompson et al., 1994; Nucleic Acids Research 22: 4673-4680). For example, the phylogenetic tree was created using the phylogenetic tree creation software of Kumar et al., 2004; Briefings in Bioinformatics 5: 150-163). For estimation of the phylogenetic tree, the neighborhood connection method (see, for example, Saitou and Nei, 1987; Molecular Biology and Evolution 4: 406-425) is used, and the reliability of each phylogenetic branch is determined by the bootstrap method (for example, Felsenstein, 1985; Evolution). 39: 783-791).
The created molecular phylogenetic tree is shown in FIG. In FIG. 10, the 28S rDNA-D1 / D2 base sequence (Table 3, previously described) of yeast isolated from the specimen is shown as “Table 3”.

  As a result, the 28SrDNA-D1 / D2 base sequence obtained from the yeast isolated from the specimen on the YMA medium showed a high homology with the base sequence of Rhodotorula mucilaginosa, which is a kind of basidiomycetous yeast. In addition, in the phylogenetic tree created based on the obtained sequence (FIG. 10), R.I. including the reference strain of mucilaginosa (CBS316; accession number AF070432). A large phylogenetic tree identical to the mucilaginosa group was formed.

From the above results, the yeast isolated from the jellyfish waste liquid-utilizing microorganism group specimen was identified as a bacterial species belonging to Rhodotorula mucilaginosa.
The Rhodotorula mucilaginosa 126-Aege strain separated and stored from the specimen in Example 3 was registered with the Patent Organism Depositary (IPOD) in the National Institute of Advanced Industrial Science and Technology as of April 21, 2006. Deposited as FERM P-20896.

(4) Identification of bacteria group in jellyfish waste liquid utilization microorganism group <DGGE analysis>
As a result of the shaking culture for 7 days in the above (2), the jellyfish was further collected with respect to the sample (the sea mud collected at a depth of 5 cm from the seabed was accumulated and cultured in the jellyfish waste liquid for 7 days). Using a culture solution (including many jellyfish waste liquid-use microorganisms) repeatedly accumulated and cultured in waste liquid for 3 to 4 days, the bacterial group contained in the specimen is analyzed using DGGE (denaturing agent gradient gel electrophoresis) as follows: Analysis was performed as described above.
The precipitate was collected from the specimen by centrifugation at 15,000 rpm for 20 minutes. Using this precipitate, DNA derived from bacteria in the specimen was extracted based on the method of Nagashima et al. (See Food Sci. Technol. Res. 2000, 6, 115-118).
About 200 bp (E. coli No. 341-534) of 16S rDNA (see Food Sci. Technol. Res. 2000, 6, 115-118) was amplified by PCR using the extracted DNA as a template. The used primers are as follows.

[Primers used]
GC-341f: 5′-GC clamp—CCTACGGGAGGCAGCAG-3 ′ (SEQ ID NO: 8)
534r: 5′-ATTACCCGCGCTGCTGGG-3 ′ (SEQ ID NO: 9)
(GC clamp: CGCCCGCCGCGCGCGGGCGGGGGGGGCCGGGGGGG)

  AmpliTaq Gold (Applied Biosystems) was used for the DNA polymerase, and GeneAmp PCR System 9600 (Applied Biosystems) was used for the thermal cycler. PCR conditions are 94 ° C., 7 minutes + (94 ° C., 60 seconds / 65 ° C. to 55 ° C., 60 seconds / 72 ° C., 120 seconds) × 20 cycles (set so that the annealing temperature decreases by 1 ° C. every 2 cycles) + (94 ° C., 60 seconds / 55 ° C., 60 seconds / 72 ° C., 120 seconds) × 15 cycles + 72 ° C., 10 minutes.

In order to confirm that the target sequence was amplified by PCR, the PCR product was electrophoresed on a 2% agarose gel. An amplified fragment electrophoresed in an agarose gel under ultraviolet irradiation was compared with a 100 bp DNA ladder (TOYOBO) as a marker, and an amplified fragment having a target length was confirmed.
The DGGE analysis was performed using a Dcode DGGE complete system (BIORAD). The denaturant concentration gradient was 30% → 60% in the electrophoresis direction, and the polyacrylamide gel concentration was 10%. Urea (BIORAD) and formamide (BIORAD) were used as the gel denaturant, and the electrophoresis conditions were a voltage of 200 V and a migration time of 3.5 hours.
After the electrophoresis, the denaturant concentration gradient gel was stained with Syber Green (Takara Bio Inc.), and excitation light emitted from the stained DNA under UV (310 nm) irradiation was photographed with a CCD camera. The obtained DGGE electrophoresis image is shown in FIG.
As a result of DGGE analysis, six types of bands (FIG. 11, a to f) showing DNA fragments of the bacterial group contained in the specimen were detected. Each band was further subjected to the following base sequence analysis and molecular phylogenetic analysis.

<Base sequence analysis>
Base sequence analysis was performed on each of the DNA fragments of the six bands a to f (FIG. 11) obtained by the DGGE analysis, and the attribution taxon of the strain from which each band was derived was estimated.
First, the purity of each band was confirmed by performing DGGE analysis again. The DGGE conditions were the same as described above. Subsequently, using the following analysis tool, the base sequence of each band was decoded by a known method, and the obtained base sequence was subjected to homology search.

[Band base sequence analysis tool]
Cycle sequence: BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems)
Sequence: ABI PRISM 3100 Genetic Analyzer System (Applied Biosystems)
Primers used: GC-341f, 534r (see Appl. Environ. Microbiol. 1993, 59, 695-700)
Analysis software: Auto Assembler (Applied Biosystems), DNASIS Pro (Hitachi Software Engineering)
Homology search: International base sequence database (GenBank / DDBJ / EMBL)

  The results of estimating the assignment of highly homologous sequences in the bands a to f from the results of the homology search are shown in Tables 4 to 9 below. The number (%) in parentheses indicates the homology rate obtained by the homology search.

From the results in Table 4, band a was estimated to be a sequence derived from a strain belonging to Sphingobacterium.
From the results of Table 5, band b was estimated to be a sequence derived from a strain belonging to Sphingobacterium.
From the results in Table 6, band c was estimated to be a sequence derived from a strain belonging to Pseudomonas.
From the results in Table 7, band d was estimated to be a sequence derived from a strain belonging to the Bacteroidetes gate.
From the results in Table 8, band e was estimated to be a sequence derived from a strain belonging to the Bacteroidetes gate.
From the results in Table 9, band f was estimated to be a sequence derived from a strain belonging to Bacillus.

<Molecular phylogenetic analysis>
Based on the results of the base sequence analysis, molecular phylogenetic analysis was performed using 16S rDNA of a close group of bacterial species suggested to be related to the strains indicated by the bands a to f, and the attribution classification of each band Groups were further estimated.
16S rDNA derived from a reference strain in a related group estimated from the results of base sequence analysis of 6 bands a to f from the international base sequence database (GenBank / DDBJ / EMBL) and the bacterial reference strain database (Techno Suruga) And created a molecular phylogenetic tree. In addition, the region used for creating the phylogenetic tree was approximately 200 bp (E. Coli No. 341-534) of 16S rDNA in order to match the amplification range of the primer used in the DGGE analysis. Analysis software includes DNASIS Pro (Hitachi Software Engineering), CLUSTAL W (see, for example, Nucleic Acids Research, 1994, 22: 4673-4680), and MEGA ver. 3.1 (for example, Briefings in Bioinformatics, 2004, 5, 150-163).

Bands (a, b, d, e) derived from Sphingobacterium and Bacteroidetes were combined as one phylogenetic tree, and the other bands (c, f) were prepared as one phylogenetic tree. Each phylogenetic tree is shown in FIGS.
12 to 14, numbers near the branch of the branch are bootstrap values (see, for example, Evolution, 1985, 39: 783-791), and a lower left line indicates a scale bar. T at the end of the stock name indicates that type of strain (Type strain).

From the results of the base sequence analysis, bands a, b, d, and e were suggested to be attributed to Bacteroidetes, so that 16S rDNA data derived from a larger number of genera belonging to Bacteroidetes, centered on Sphingobacterium. And a molecular phylogenetic tree was created (FIG. 12).
Also, since bands c and d are suggested to belong to Pseudomonas and Bacillus, respectively, 16S rDNA data derived from many reference strains belonging to Pseudomonas and Bacillus were obtained, and a molecular phylogenetic tree was created (FIG. 13). 14).

As a result of molecular phylogenetic analysis, 16S rDNA in band a formed a phylogenetic branch with 16S rDNA of Sphingobacterium multivolum IAM14316 ^T (AB100738), and thus was estimated to be closely related to Sphingobacterium multivorum (FIG. 12).
Further, 16SrDNA of band b formed a phylogenetic branch with 16SrDNA of Sphingobacterium spiritivorum ATCC 33861 ^T (M58778), which was a reference species, and thus was assumed to be closely related to Sphingobacterium spiritivorum (FIG. 12).
Both of these two bands have a high bootstrap value (see Evolution, 1985, 39: 783-791) indicating the reliability of the phylogenetic branch (branch), which is considered to have high stability of the phylogenetic tree.

As a result of the DGGE band base sequence analysis, the 16S rDNAs of the bands d and e, which have been designated as Bacteroidetes, are not included in the same cluster as Flavobacterium aquatile ATCC 11947 ^T (M62797), but belong to Flavobacterium Species-derived sequences and phylogenetic branches were formed (FIG. 12). Therefore, these bands were presumed to be sequences derived from a strain belonging to Flavobacterium.

The 16S rDNA of band c is composed of Pseudomonas rhizosphaerae IH5 ^T (AY152673), Pseudomonas flavescens B62 ^T (U01916), Pseudomonas graminis DSM11363 ^T (Y11150) and 16 SrDNA which form Pseudomonas graminis DSM11363 ^T (Y11150). It was presumed to be a sequence derived from a strain belonging to Pseudomonas, which is closely related to 16SrDNA.

Although the 16S rDNA of the band f is not included in the same cluster as the reference species Bacillus subtilis IAM12118 ^T (AB042061), it forms a phylogenetic branch with the 16S rDNA of Bacillus pumilus ATCC7061 ^T (AY87289) (FIG. 14). Presumed to be closely related to Bacillus pumilus 16SrDNA.

  The assigned taxonomic groups estimated from the results of molecular phylogenetic analysis of the obtained bands are summarized in Table 10.

From the above results, in the jellyfish waste liquid-utilizing microorganism group sample in the sea mud, bacteria belonging to the genus Sphingobacteria, bacteria belonging to the genus Pseudomonas, bacteria belonging to the genus Flavobacterium, And it turned out that the bacteria group which consists of bacteria which belong to Bacillus (Bacillus) genus is contained.
Still further, the bacterium belonging to the genus Sphingobacteria includes at least one of Sphingobacterium multivolum and Sphingobacterium spiritivorum, and belongs to the genus Bacillus. It has been found that bacteria include Bacillus pumilus.

(Example 4: Basic examination of waste liquid treatment capacity of each microorganism group-immobilized carrier)
In Example 4, the microorganisms suitable for the treatment of the salt-containing waste liquid identified in Example 3, that is, the yeast and the bacteria group, were separately or mixed and immobilized on a carrier, and the following (1) ) To (3), a basic study was conducted on the waste liquid treatment capacity of each microorganism group-immobilized carrier.
The combinations subjected to the comparative study in Example 4 are as shown in the following I) to IV). “Yeast + bacteria group” indicates that a carrier on which both yeast and bacteria group are immobilized was used.
I) Yeast-Bacterial group II) Yeast + Bacterial group-Yeast III) Yeast + Bacterial group-Bacterial group IV) Ozone treatment-Ozone untreated (both used yeast + bacteria group)

(1) Preparation of each microorganism group immobilization carrier (pellet) Each microorganism group immobilization carrier (pellet) was prepared as follows. The microorganism to be entrapped and immobilized is a group of bacteria (the sea mud collected at a depth of 5 cm from the seabed in Example 3 (2) above and accumulated and cultured in a jellyfish waste solution for 7 days. The jellyfish waste liquid-use microorganism group centering on bacteria obtained by repeated culture for 4 days and yeast (Rhodotorula mucilaginosa) isolated and stored in Example 3 above were further cultured in the jellyfish waste liquid for 3 to 4 days. The jellyfish waste liquid use yeast) was used alone or in combination.

Below, the preparation method of a pellet is described, taking the example of the preparation of the pellet which carried out the comprehensive fixation of the jellyfish waste liquid utilization yeast.
Jellyfish waste liquid (100 ml) was placed in a 500 ml rotary flask. Next, the jellyfish waste liquor-utilizing yeast is inoculated into the jellyfish waste liquor from the slant medium separated and stored in Example 2 (2) above, using a platinum sterilized flame-sterilized under aseptic conditions. did. Thereafter, shaking culture (180 rpm) was performed for 3 to 4 days to increase the number of bacteria.
Next, the culture solution was transferred to a centrifuge tube, centrifuged (10,000 rpm, 10 min, 4 ° C.), and the supernatant was removed. After removing the supernatant, distilled water (20 ml) was added to the centrifuge tube, mixed with the precipitate containing the jellyfish waste liquid utilizing yeast, and suspended. Next, agar (6 g) and distilled water (180 ml) were stirred and mixed in a 200 ml beaker and dissolved by heating.
After the agar lysis, the temperature of the agar lysate was lowered to 55 ° C. in order to prevent the mixed microorganisms from being killed, and a mixture of precipitates (20 ml) containing jellyfish waste liquid utilizing yeast was added and stirred. After agitation, agar suspended in yeast was injected into a silicone tube (36 tubes) having an inner diameter of 6 mm using a 10 ml syringe. After the injection, the mixture was left in a low temperature room at 6 ° C. for 10 minutes in order to quickly solidify the agar. After standing, agar (thin string-like shape) was extruded from the silicon tube using a stainless steel rod, and cut at intervals of about 3 mm using a stainless steel spatula. The cut agar (hereinafter referred to as pellet) was stored in a beaker with jellyfish waste liquid at 4 ° C.

The pellets in which the bacterial group was entrapped and immobilized, and the pellets in which both the yeast and the bacterium group were entrapped and immobilized, were prepared in the same manner as in the case of the yeast.
In addition, when both yeast and bacteria groups were entrapped and immobilized together, both were prepared so as to be equal in amount as the amount of the mixed solution added to the agar.

(2) Basic examination of waste liquid treatment with entrapped immobilized microorganisms 500 ml of jellyfish waste liquid obtained in the same manner as in Example 3 was put into two 1 L Erlenmeyer flasks, capped, sterilized by autoclave, and jellyfish A waste liquid was prepared. In this example, in order to eliminate the influence of microorganisms present in the jellyfish waste liquid from the beginning, the jellyfish waste liquid was autoclaved as described above. In addition, for items other than IV), the jellyfish waste liquid was pretreated with ozone according to the method described in Example 1 (1).

  Into the jellyfish waste liquid prepared as described above, pellets (200 ml) in which microorganisms of each item were comprehensively immobilized were placed. Thereafter, the flask was aerated in an incubator (32 ° C.), and the waste liquid was treated with each entrapping immobilized microorganism under aerobic conditions. For each of the combination elements I) to IV), the following evaluation items (COD, total nitrogen, total phosphorus, residual chlorine, pH) were evaluated and compared.

[Measurement of COD]
The COD was measured with a digital reactor block / DRB200 type (hereinafter referred to as a digital block) compliant with Japanese Industrial Standards (JIS). 1 ml of a measurement sample was taken in an Eppendorf tube, and centrifuged (15,000 rpm, 10 min, 4 ° C.) to remove solids. After centrifugation, 30 μl of the obtained sample supernatant was placed in a test tube by centrifugation, and 5,970 μl of MilliQ water was added to prepare a 200-fold diluted sample of the sample stock solution. After preparing the diluted sample, put the diluted sample (5 ml) into a test tube (COD measurement kit, glass tube with cap), and add reagent A solution (COD measurement kit, KMnO ₄ solution) (0.5 ml), The cap was closed and mixed by inversion. After mixing by inversion, the reaction was performed with a digital block at 100 ° C.
After 30 minutes, the test tube was removed from the digital block and cooled on ice. The cooling of the test tube was confirmed, and centrifugation (3,500 rpm) was performed to remove solid matter in the tube. The zero point adjustment was performed with a water quality analyzer (DR / 2400 type) using Milli-Q water as a control. Next, COD measurement was performed on the test tube containing the measurement sample.

[Measurement of total nitrogen (TN)]
The measurement of TN was performed with a digital block conforming to Japanese Industrial Standards (JIS). 0.5 ml of the measurement sample was put in a test tube (TN measurement kit, glass tube). The control was 0.5 ml of milli-Q water. A total nitrogen persulfate reagent powder pillow (TN measurement kit reagent) was mixed with the measurement sample, and the tube was mixed by inversion. The reaction was carried out with a digital block at 105 ° C.
After 30 minutes, the test tube was removed from the digital block and allowed to stand at room temperature. Thereafter, nitrogen reagent [A] (TN measurement kit reagent) was added, the cap was closed, and the tube was mixed by inversion. Three minutes later, total nitrogen reagent [B] (TN measurement kit reagent) was added, the cap was closed, and the tube was mixed by inversion. Two minutes later, the sample (2 ml) to which the total nitrogen reagent [A · B] was added as described above was mixed in a TN reagent C vial (TN measurement kit, test tube). Thereafter, the TN reagent C vial was thoroughly mixed by inversion. And the zero point adjustment was performed by the control | contrast and TN of the measurement sample was measured.

[Measurement of total phosphorus (TP)]
The measurement of TP was performed with a digital block conforming to Japanese Industrial Standards (JIS). 5 ml of the measurement sample was put in a test tube (TP measurement kit, glass tube). The control was 5 ml of Milli-Q water. After mixing the measurement sample, potassium persulfate powder pillow for phosphate (TP measurement kit reagent) was added, and the tube was mixed by inversion. The reaction was carried out with a digital block at 150 ° C.
After 30 minutes, the test tube was removed from the digital block and allowed to stand at room temperature. 1.54N NaOH (2 ml) was added and mixed by inversion. One minute later, a molybdenum vanadate reagent (0.5 ml) was mixed and mixed by inversion. Thereafter, the zero point was adjusted with the control, and the test tube was centrifuged (3,500 rpm) for 5 minutes, and TP of the measurement sample on which the suspended substance was precipitated was measured.

[Measurement of residual chlorine (Cl)]
The Cl was measured with a digital block in accordance with Japanese Industrial Standard (JIS). 10 ml of the measurement sample was placed in a glass tube. For control purposes, another 10 ml of the above measurement sample was placed in a glass tube. Thereafter, DPD free chlorine powder (Cl measurement kit reagent) was added to the measurement sample, and the tube was mixed by inversion. And zero point adjustment was performed by the control | contrast which does not mix DPD free chlorine powder, and measured Cl of the measurement sample.

[Measurement of pH]
The pH was measured with a pH meter.

(3) Results I) Yeast-bacteria group [COD reduction rate]
The maximum COD reduction rate when the yeast was cultured in the jellyfish waste solution was 53.1% (2,560 ppm → 1,200 ppm) on the seventh day. In contrast, the reduction rate of the bacterial group alone was 46.2% (2,600 ppm → 1,400 ppm) on the seventh day. In 7 days, the difference in the COD reduction rate when the yeast group and the bacterial group were cultured was 6.9%. The COD reduction rate 3 days after the start of the culture was 23.4% for the yeast and 4.6% for the bacterial group (FIG. 15).
[Total nitrogen (TN) reduction rate]
The maximum TN reduction rate when yeast was cultured in the jellyfish waste solution was 64.3% (73 ppm → 26 ppm) on the seventh day. In contrast, the reduction rate of the bacterial group alone was 56% (72 ppm → 32 ppm) on the seventh day. In 7 days, the difference in the TN reduction rate when the yeast and the bacterial group were cultured was 8.3% (FIG. 16). The TN decrease rate 2 days after the start of the culture was 49.4% for the yeast and 19.4% for the bacterial group.
[Total phosphorus (TP) reduction rate]
The maximum TP reduction rate when yeast was cultured in the jellyfish waste solution was 100% (26 ppm → 0 ppm) on the seventh day. Similarly, the reduction rate of the bacterial group alone was 100% (25.9 ppm → 0 ppm). In 7 days, there was no difference in the TP decrease rate when the yeast and the bacterial group were cultured. Two days after the start of the culture, the TP decrease rate was 39.61% for the yeast and 17.7% for the bacterial group (FIG. 17).
[Residual chlorine (Cl) reduction rate]
When the yeast was cultured in the jellyfish waste solution, the maximum Cl reduction rate was 37.7% on the seventh day (0.61 ppm → 0.38 ppm). The maximum reduction rate of the bacterial group alone was 32.8% (0.67 ppm → 0.45 ppm) on the second day. In 7 days, the difference in culturing the yeast and bacterial groups was 43.6% (FIG. 18).
[PH]
Changes in pH after the start of operation were measured over time (FIG. 19). The pH of the yeast changed from 5.89 to 4.52. On the other hand, the pH of the bacterial group changed from 5.74 to 4.56. Both changed to the acidic side as well, and the final pH showed almost the same value.
[Sludge generation amount]
In addition, Table 11 below shows the amount of sludge generated during the treatment of the waste liquid with the pellets according to the combination element conditions. In the yeast-bacteria group of I), the amount of sludge generation was 89.8% less in the yeast group than in the bacterial group (Table 11).

II) Yeast + Bacteria group-Yeast [COD reduction rate]
The maximum COD reduction rate when the yeast + bacteria group was cultured in the jellyfish waste solution was 48.8% (1,620 ppm → 830 ppm) on the seventh day. The decrease rate of yeast only on the 7th day was 46.1% (1,650 ppm → 890 ppm). In 7 days, the difference between the yeast + bacteria group and the yeast was 2.7% (FIG. 20). One day after the start of the culture, the COD reduction rate was 32.7% in the yeast + bacteria group, and 52.7% in the yeast.
[Total nitrogen reduction rate]
The maximum TN reduction rate when the yeast + bacteria group was cultured in the jellyfish waste solution was 4.6% (24 ppm → 22.9 ppm) on the third day. In yeast alone, it was 24.9% (23.7 ppm → 17.8 ppm) on the second day. (FIG. 21). The TN reduction rate 2 days after the start of the culture was 24.9% for yeast versus 0% for the yeast + bacteria group.
[Total phosphorus reduction rate]
The maximum TP reduction rate when the yeast + bacteria group was cultured in the jellyfish waste solution was 100% (13.6 ppm → 0 ppm) on the seventh day. Similarly, the reduction rate with only yeast was 100% (11.3 ppm → 0 ppm). There was no difference between yeast + bacteria group and yeast in 7 days (FIG. 22). The TP reduction rate 3 days after the start of the culture was 4.6% for yeast versus 46.9% for the yeast + bacteria group.
[Residual chlorine reduction rate]
When the yeast + bacteria group was cultured in the jellyfish waste solution, the maximum Cl reduction rate was 100% on the first day (0.25 ppm → 0.00 ppm). Similarly, with yeast alone, the reduction rate was 100% (0.5 ppm → 0 ppm) on the first day (FIG. 23). The Cl reduction rate one day after the start of the culture was 100% of the yeast + bacteria group, and the yeast was 100%, and there was no difference.
[PH measurement]
Changes in pH after the start of operation were measured over time (FIG. 24). The pH of the yeast + bacteria group changed from 7.26 to 7.44. On the other hand, the pH of the yeast changed from 7.01 to 7.42. In both cases, the pH was almost neutral.
[Sludge generation amount]
Yeast + bacteria group—Yeast alone produced 42.4% less sludge than yeast + bacteria group (Table 11, above).

III) Yeast + bacteria group-bacteria group [COD reduction rate]
The maximum COD reduction rate when the yeast + bacteria group was cultured in the jellyfish waste solution was 41.4% (1,400 ppm → 820 ppm) on the third day. The reduction rate of the bacterial group alone was 41.8% (1,340 ppm → 780 ppm) on the seventh day (FIG. 25). The COD reduction rate after 3 days from the start of the culture was 41.4% for the yeast + bacteria group, but 3.0% for the bacteria group.
[Total nitrogen reduction rate]
The maximum TN reduction rate when the yeast + bacteria group was cultured in the jellyfish waste solution was 97.5% (80 ppm → 2.0 ppm) on the third day. The reduction rate of the bacterial group alone was 88.5% (61 ppm → 7.0 ppm) on the second day (FIG. 26). The TN reduction rate 3 days after the start of the culture was 97.5% for the yeast + bacteria group, whereas it was 77.0% for the bacteria group.
[Total phosphorus reduction rate]
The maximum TP reduction rate when the yeast + bacteria group was cultured in the jellyfish waste solution was 100% (21.3 ppm → 0 ppm) on the third day. The reduction rate of the bacterial group alone was 100% on the seventh day (21.3 ppm → 0 ppm). There was no difference between the yeast + bacteria group and the bacteria group in 7 days (FIG. 27). The TP reduction rate 3 days after the start of the culture was 62.0% for the bacteria group compared to 100% for the yeast + bacteria group.
[Residual chlorine reduction rate]
The maximum Cl reduction rate when the yeast + bacteria group was cultured in the jellyfish waste solution was 100% (0 ppm → 0 ppm) on the first day. Similarly, the reduction rate of only the bacterial group was 100% (0.26 ppm → 0.00 ppm). In 7 days, the difference between the yeast + bacteria group and the bacteria group was 30.8% (FIG. 28). The Cl reduction rate 2 days after the start of the culture was 100% for the yeast + bacteria group, whereas it was 53.8% for the bacteria group.
[PH measurement]
The change in pH after the start of operation was measured over time (FIG. 29). The pH of the yeast + bacteria group changed from 4.27 to 6.13. The pH of the bacterial group was changed from 4.26 to 5.65. Both changed to the alkaline side, and the final pH showed almost the same value.
[Sludge generation amount]
Yeast + Bacterial Group-In the bacterial group, sludge generation was 52.5% less in the yeast + bacterial group than in the bacterial group (Table 11, above).

IV) Ozone treatment zone-Ozone untreated zone [COD reduction rate]
The maximum COD reduction rate when the yeast + bacteria group was cultured in the jellyfish waste solution was 74.9% (3,920 ppm → 985 ppm) on the sixth day in the ozone treatment group. Similarly, the decrease rate of the ozone-untreated section was 69.0% (3,480 ppm1 → 1,080 ppm). In 6 days, the difference between the ozone-treated area and the untreated area was 5.9% (FIG. 30).
[Total nitrogen reduction rate]
The maximum TN reduction rate when the yeast + bacteria group was cultured in the jellyfish waste solution was 100% (148 ppm → 0 ppm) on the sixth day in the ozone treatment group. Similarly, the decrease rate of the ozone-untreated section was 100% (156 ppm → 0 ppm). There was no difference between the ozone-treated area and the untreated area in 6 days (FIG. 31).
[Total phosphorus reduction rate]
The maximum TP reduction rate when the yeast + bacteria group was cultured in the jellyfish waste solution was 100% (42.7 ppm → 0 ppm) on the sixth day in the ozone treatment group. The decrease rate of the ozone-untreated section was 73.6% (43.6 ppm → 11.5 ppm). In 6 days, the difference between the ozone-treated area and the untreated area was 26.4% (FIG. 32). Two days after the start of the culture, the TP reduction rate was 12.4% in the ozone-treated group and 2.3% in the untreated group.
[Residual chlorine reduction rate]
The maximum Cl reduction rate when the yeast + bacteria group was cultured in the jellyfish waste solution was 100% (0.99 ppm → 0 ppm) on the second day in the ozone treatment group. Similarly, the decrease rate of the ozone-untreated section was 100% (0.79 ppm → 0 ppm), and there was no difference between the ozone-treated section and the untreated section (FIG. 33).
[PH measurement]
The change in pH after the start of operation was measured over time (FIG. 34). The pH of the ozone treatment changed from 4.99 to 6.82. And the pH of ozone non-treatment changed from 5.06 to 6.21. Moreover, both changed to the alkali side and the final pH showed almost the same value.
[Sludge generation amount]
Ozone treatment—The amount of sludge generated in the untreated ozone was 31.2% less than in the untreated ozone (Table 11, above).

As described above, according to Example 4, the yeast, Rhodotorula mucilaginosa identified in Example 3, can reduce organic matter, nitrogen, phosphorus, and residual chlorine in the jellyfish waste liquid particularly efficiently, and has a salinity concentration. It was shown to be very effective in the treatment of jellyfish waste liquid with a high organic matter concentration (FIGS. 15 to 29).
Further, from the results of Table 11, it was observed that the yeast can remarkably reduce the amount of sludge generated in the jellyfish waste liquid treatment compared to the bacterial group (I). In comparison, it was observed that sludge can be reduced by about 50% (IV). Since Rhodotorula mucilaginosa produces polysaccharide mannan, which is a viscous substance, outside the cells, it is presumed that the mannan agglomerates inorganic substances and floating substances in the waste liquid to enhance the effect of the waste liquid treatment. By the formation of flocs by aggregation of the inorganic substances and suspended solids, it is possible to prevent microorganisms from flowing out of the treatment process, and further, biofilms are formed in the treatment tank and in the bioreactor. It is thought that the microbial surface area of can be greatly increased.

  The yeast, Rhodotorula mucilaginosa, produces carotenoids, and the culture becomes reddish orange (see, for example, Folia Microiol., 49; 2004; 19-25). Since the production of pigments is reduced by mixed culture with bacterial groups, the combined use of the yeast and bacterial groups is also advantageous for clarifying the treatment solution after the coagulation-precipitation treatment. It is also shown from the results of this Example 4 that the treatment method using both yeast and bacteria group is also very effective for jellyfish waste liquid treatment (FIGS. 15 to 29).

  In addition, the remarkable difference with respect to a jellyfish waste liquid process was not recognized by the case where it does not perform with the case where ozone treatment is performed (FIGS. 30-34). Therefore, it was also confirmed from this that each of the above-mentioned effects on the jellyfish waste liquid treatment was not due to the effect of the ozone treatment, but due to the action of each microbial group. However, according to the ozone treatment, since organic substances such as lipids and polysaccharides that could not be decomposed by the prionase treatment are further decomposed by ozone, this ozone treatment may be performed in order to make it easier for microorganisms to use the waste liquid. It is also considered advantageous.

(Example 5: Waste liquid treatment by bioreactor using yeast + bacteria group immobilization support)
(1) Preparation of Yeast + Bacterial Group Immobilization Carrier A pellet in which both the yeast and the bacterial group were comprehensively immobilized was prepared in the same manner as in Example 4. In addition, the amount ratio between the yeast and the bacterial group was adjusted so that the amount of the mixed solution added to the agar was equal to each other as described above.

(2) Waste liquid treatment <Equipment>
Processing was performed using the apparatus of the present invention shown in FIG. In FIG. 1, 1 is a wastewater tank, 2 is a transfer pump, 3 is a three-way cock, 4 is a venting device (dissolved oxygen concentration adjusting means), 5 is a bioreactor (container), 6 is an electrolysis tank (sterilizing means), 7 Indicates a gate valve.
In the bioreactor, a stainless steel (SUS304) cage (1 mm mesh) was installed to hold the pellets. In the bioreactor, 90% of the pellet prepared in the above (1) was filled in a basket previously placed in the bioreactor so that the whole pellet was vented.
Further, in the jellyfish waste liquid prepared in the same manner as in Example 3 (1), 0.1% of the jellyfish waste liquid amount of glucose and the salt concentration of seawater are 3.0% (w / v). An amount of artificial seawater was added, and the temperature of the wastewater tank was set to a temperature at which the microbial community actively grew (32 ° C.).

<Waste liquid treatment>
The jellyfish waste liquid was treated in the following steps. The jellyfish waste liquid was transferred from the waste water tank into the bioreactor by the operation of the three-way cock. In addition, each bioreactor was connected by a silicon tube so that the jellyfish waste liquid passed through all three bioreactors. Then, the waste liquid that once passed through the bioreactor returned to the waste liquid tank, and the waste liquid was treated by a circulation process that was transferred again into the bioreactor. Further, air generated by an air pump (aeration device) was aerated in the bioreactor in order to make the bioreactor aerobic (FIG. 1). The treatment of the jellyfish waste liquid by the bioreactor was performed for 7 days in continuous operation.
The operating conditions of the apparatus are shown below.
[Operating conditions]
Pump model: CAS-1
Electricity use: 100V 50 / 60Hz
Current consumption: 1.8 / 1.4A
No load flow rate: 9.2 / 11 LPM
Maximum pressure: 2.3 bar or more Maximum vacuum: 292 mbar or more Port size: 8 mm

<Precipitation and filtration>
Seven days later, the 8 L volume of jellyfish waste liquid after the waste liquid treatment was transferred to a coagulation sedimentation apparatus, and 20 g of calcium hydroxide was added. After 1 hour, the supernatant of the aggregated precipitate was collected. Then, the supernatant obtained by coagulation precipitation was passed through an activated carbon column, and the jellyfish waste liquid was filtered.

(3) Waste liquid treatment results The following items were evaluated in the same manner as in Example 4. The results are shown below.
In addition, the measurement of suspended solids (SS) was performed using the digital block based on the Japanese Industrial Standard (JIS). 25 ml of the measurement sample was put in a glass tube dedicated to SS measurement. Milli-Q water was used as a control sample, zero point adjustment was performed, and SS of the measurement sample was measured.
[COD measurement results]
The COD decreased by 86.2% (2,320 ppm → 320 ppm) on the 4th day of the first treatment by the bioreactor and 88.7% (2,320 ppm → 260 ppm) in 7 days (FIG. 35). As a result of coagulation precipitation as a secondary treatment, the reduction rate was 89.7%, and as a result of performing activated carbon filtration, the reduction rate was 99.9% (FIG. 35).
In addition, after the start of the bioreactor operation, the waste liquid was changed every other week, and the COD reduction rate after the seventh day was examined. As a result, the first week was 86.0% (1,680 ppm → 235 ppm), the second week was 88.5% (3,440 ppm → 395 ppm), and the third week was 88.7% (2,320 ppm). → 260 ppm), the COD reduction efficiency increased as the number of repetitions increased. (FIG. 36).
[Total nitrogen (TN) measurement results]
On the second day of the primary treatment with the bioreactor, 92.7% (31 ppm → 3 ppm) and 90.2% (41 ppm → 4 ppm) TN decreased in 4 days (FIG. 37). Moreover, the reduction rate became 100% by the secondary treatment by coagulation sedimentation and activated carbon filtration (FIG. 37).
[Total phosphorus (TP) measurement results]
90% (40.1 ppm → 4 ppm) on the 4th day of the primary treatment by the bioreactor, and 80.5% (40.1 ppm → 7.8 ppm) TP decreased in 7 days (FIG. 38). In addition, as a result of coagulation precipitation and activated carbon filtration as a secondary treatment, the reduction rate was 98.8% (FIG. 38).
In addition, a reduction rate of almost 100% was observed every time.
[Residual chlorine (Cl) measurement results]
On the third day of the primary treatment by the bioreactor, 100% (0.85 ppm → 0 ppm) and 83.5% (0.85 ppm → 0.14 ppm) Cl decreased in 7 days (FIG. 39). In addition, as a result of coagulation precipitation and activated carbon filtration as a secondary treatment, the reduction rate was 82.4% (FIG. 39).
[Suspended matter (SS) measurement result]
19% (100 ppm → 81 ppm) SS decreased in 7 days of the primary treatment with the bioreactor (FIG. 40). Moreover, as a result of carrying out aggregation precipitation and activated carbon filtration as the secondary treatment, the reduction rate was 100% (FIG. 40).
[PH measurement result]
The average value of pH change (10 months) was determined (FIG. 41). In addition, the reason why the pH became alkaline in the coagulation sedimentation in the primary treatment is that the coagulation sedimentation was performed with calcium hydroxide.

[Changes in pellet morphology]
One month after the operation, the pellet in the reactor was translucent to clouding indicating microbial growth (FIG. 42). Moreover, as a result of microscopic observation, yeasts, bacteria, and protozoa were present in a well-balanced manner in the microorganisms in the entrapped and immobilized pellets (FIG. 43).
[Appearance of waste liquid for each process]
In addition, FIG. 44 shows the jellyfish waste liquid after ozone treatment (before treatment), the waste liquid after bioreactor treatment (primary treatment), the waste liquid after aggregation precipitation treatment (aggregation precipitation), and the waste liquid after activated carbon filtration (activated carbon filtration) in order from the left. . The coagulation sediment waste liquid was a slightly colored liquid, but became a completely transparent liquid by activated carbon filtration.
[Examination of coagulant used for coagulation sedimentation]
45 to 46 show the results of preliminary examination of the flocculant used for the coagulation sedimentation treatment. When calcium oxide (quick lime) (Fig. 45, second from the right), calcium hydroxide (slaked lime) (Fig. 45, right end), sodium aluminate (Fig. 45, fourth from the left) is added as a coagulating precipitant In this case, the upper layer waste liquid became transparent, and it took time for sodium aluminate to precipitate.

As a result, according to the bioreactor single treatment filled with pellets in which yeast and bacteria were entrapped and immobilized, within 4 days, the measurement values (reduction rate) of each item treated in the flask in Example 4 for 7 days were reached. A decreasing effect was observed. Therefore, the effect of the bioreactor treating waste liquid in a short period was recognized.
In addition, it can be seen from the results of FIG. 36 that even when the bioreactor is continuously operated, the COD reduction rate is not inferior, and conversely, the COD reduction rate increases as the number of operations is repeated. This is presumed that the microorganisms in the pellet were conditioned by the organic components in the waste liquid.
Moreover, the COD measurement result (FIG. 35) in Example 5 (treatment of prionase enzyme-treated jellyfish waste liquid) is compared with the COD measurement result (FIG. 2) in Example 1 (treatment of hydrothermal treatment jellyfish waste liquid). As a result, in Example 5, the COD reduction effect almost the same as that of Example 1 was observed despite the higher COD value in the jellyfish waste liquid before bioreactor treatment (2,320 ppm). Therefore, the bioreactor was found to be more suitable for the treatment of enzyme-treated jellyfish waste liquid.

(Example 6: Waste liquid treatment when using a microbial group cultured in a medium other than jellyfish waste liquid)
The bacteria group (microorganism group) used in Examples 4 to 5 is a microorganism group (including yeast) obtained by accumulating microorganisms in sludge that grows using jellyfish waste liquid as a medium. Each component in the jellyfish waste liquid decreased remarkably with the passage of days (FIGS. 15 to 41). However, in Example 6, the culture medium for accumulating the microorganisms in the sludge is the medium normally used for culturing microorganisms from the jellyfish waste liquid (S medium: bacto-seuton; 1.0%, galactose; 2.0%, corn steep). Liqueur; 0.5%, dextrin; 2.0%, ammonium sulfate; 0.2%, calcium carbonate; 0.2%, pH 6.5) The reduction rate of COD during jellyfish waste liquid treatment by the obtained microorganism group (including yeast) was less than 10% even after 3 days from the start of the culture. The results are shown in FIG.
From this, the accumulation culture of the microorganism group used for the waste liquid treatment in the present invention is performed using the waste liquid to be treated (salt-containing waste liquid, for example, jellyfish waste liquid) as a culture medium, and assimilating the nutrient components in the decomposition liquid. It has been found that it is desirable not to change the nutritional components extremely even during habituation.

  The apparatuses and reagents used in Examples 3 to 5 are shown in Tables 12 to 13 below.

  According to the present invention, salt-containing waste liquids such as jellyfish decomposition waste liquids can be landed in coastal areas because they can be decomposed and disposed of at low cost, efficiently, without special equipment, and without causing environmental pollution. It is suitable for treatment of living organisms such as jellyfish, and is particularly suitable for treatment of various salt-containing waste liquids useful for preventing pollution and eutrophication of sea areas.

FIG. 1 is an example of a schematic diagram of an apparatus for treating organic waste liquid containing salt using a bioreactor according to the present invention. FIG. 2 is a graph showing the daily change in COD concentration in a wastewater treatment system using the salt-resistant organic waste liquid-use microorganism of the present invention of Example 1. FIG. 3 is a graph showing the sterilization effect in the electrolysis tank in the wastewater treatment system using the salt-resistant organic waste liquid-use microorganism of the present invention of Example 1. FIG. 4 is a schematic explanatory diagram illustrating an example of a jellyfish decomposing apparatus that is an aspect of the wet processing apparatus. FIG. 5 is a graph showing the COD reduction rate (7 days of culture) by the jellyfish waste liquid utilizing microorganism group in the seabed sludge collected from each depth. FIG. 6 is a phase-contrast micrograph image of yeast, Rhodotorula mucilaginosa, isolated from the jellyfish waste liquid-use microorganism group in Example 3. FIG. 7 is a streak culture photographic image of the yeast Rhodotorula mucilaginosa isolated from the jellyfish waste liquid-use microorganism group in Example 3. FIG. 8 is a single colony photographic image of yeast, Rhodotorula mucilaginosa, isolated from the jellyfish waste liquid-using microorganism group in Example 3. FIG. 9 is a liquid culture photographic image of yeast and Rhodotorula mucilaginosa isolated from the jellyfish waste liquid-use microorganism group in Example 3 before and after the culture. FIG. 10 is a molecular phylogenetic tree showing the attribution of the yeast, Rhodotorula mucilaginosa 126-Age strain, isolated from the jellyfish waste liquid-using microorganism group in Example 3. FIG. 11 is a DGGE electrophoresis image in which a bacterial group in the jellyfish waste liquid-using microorganism group was detected in Example 3. FIG. 12 is a molecular phylogenetic tree showing the attribution of bands a, b, d, and e detected by DGGE analysis of Example 3. FIG. 13 is a molecular phylogenetic tree showing the attribution of band c detected by DGGE analysis of Example 3. FIG. 14 is a molecular phylogenetic tree showing the attribution of the band f detected by the DGGE analysis of Example 3. FIG. 15 is a graph showing the COD reduction rate of the basic study group I of Example 4. FIG. 16 is a graph showing the total nitrogen (TN) reduction rate of the basic study group I of Example 4. FIG. 17 is a graph showing the total phosphorus (TP) reduction rate of the basic study group I of Example 4. FIG. 18 is a graph showing the residual chlorine (Cl) reduction rate of the basic study group I of Example 4. FIG. 19 is a graph showing the pH measurement results of the basic study group I of Example 4. FIG. 20 is a graph showing the COD reduction rate of the basic study group II of Example 4. FIG. 21 is a graph showing the total nitrogen (TN) reduction rate of the basic study group II of Example 4. 22 is a graph showing the total phosphorus (TP) reduction rate of the basic study group II of Example 4. FIG. FIG. 23 is a graph showing the residual chlorine (Cl) reduction rate of the basic study group II of Example 4. FIG. 24 is a graph showing the pH measurement results of the basic study group II of Example 4. FIG. 25 is a graph showing the COD reduction rate of the basic study group III of Example 4. FIG. 26 is a graph showing the total nitrogen (TN) reduction rate of the basic study group III of Example 4. FIG. 27 is a graph showing the total phosphorus (TP) reduction rate of the basic study group III of Example 4. FIG. 28 is a graph showing the residual chlorine (Cl) reduction rate of the basic study group III of Example 4. FIG. 29 is a graph showing the pH measurement results of the basic study group III of Example 4. FIG. 30 is a graph showing the COD reduction rate of the basic study group IV of Example 4. FIG. 31 is a graph showing the total nitrogen (TN) reduction rate of the basic study group IV of Example 4. 32 is a graph showing the total phosphorus (TP) reduction rate of the basic study group IV of Example 4. FIG. FIG. 33 is a graph showing the residual chlorine (Cl) reduction rate of the basic study group IV of Example 4. FIG. 34 is a graph showing the pH measurement results of the basic study group IV of Example 4. FIG. 35 is a graph showing the COD reduction rate due to the treatment using the bioreactor in Example 5. FIG. 36 is a graph showing the COD reduction rate for each week when the operation period of the bioreactor is 3 weeks in Example 5. FIG. 37 is a graph showing the total nitrogen (TN) reduction rate by the treatment using the bioreactor in Example 5. 38 is a graph showing the total phosphorus (TP) reduction rate due to the treatment using the bioreactor in Example 5. FIG. FIG. 39 is a graph showing the residual chlorine (Cl) reduction rate by the treatment using the bioreactor in Example 5. 40 is a graph showing the suspended matter (SS) reduction rate by the treatment using the bioreactor in Example 5. FIG. FIG. 41 is a graph showing the results of pH measurement by the treatment using the bioreactor in Example 5. FIG. 42 is a photographic image showing the change in color tone of the pellets before and after the bioreactor operation. FIG. 43 is a photographic image showing the diversity of microorganisms in the pellets after operation of the bioreactor. FIG. 44 is an appearance photographic image of the waste liquid after each processing step. FIG. 45 is a photographic image showing the results of examining the difference in the aggregation and precipitation effect of each reagent with respect to the waste liquid after bioreactor treatment. FIG. 46 is a photographic image showing the results of examining the difference in the aggregation and precipitation effect of each reagent with respect to the waste liquid after bioreactor treatment. FIG. 47 is a graph showing the COD reduction rate when Rhodotorula mucilaginosa is cultured in S medium.

Explanation of symbols

1 Wastewater tank 2 Transfer pump 3 Three-way cock 4 Ventilator (dissolved oxygen concentration adjusting means)
5 Bioreactor (container)
6 Electrolysis tank (sterilization means)
7 Gate valve 10 Decomposition tank 20 Partition tank 30 Siphon tube 40 Liquid feed pump 50 Circulation / warming tank 60 Gate valve 70 Gate valve 80 Heating tube 90 Heater 100 Ozone generator 110 Gate valve 120 Check valve 130 Gate valve 140 Partition Valve 150 Activated carbon treatment tube 160 Jellyfish decomposition treatment water 170 Siphon tube passage treatment liquid outlet 180 Activated carbon treatment tube passage clean waste water

Claims (14)

A carrier and at least immobilize either microorganisms yeast and bacteria, and contact means for contacting the decomposition waste jellyfish decomposed by Purionaze enzyme to the carrier, decomposition waste jellyfish which is brought into contact with said contact means A coagulation-precipitation means for aggregating the treatment liquid, and a filtration means for removing the precipitate formed by the aggregation-precipitation means,
The yeast is Rhodotorula mucilaginosa,
The bacteria group belongs to the genus Sphingobacterium, Pseudomonas rhizosphaerae, Pseudomonas flavesens, Pseudomonas flavescens, Pseudomonas flavums, An apparatus for treating a jellyfish decomposition waste liquid , which is at least one selected from the group consisting of Bacillus pumilus . Carrier fixing the microorganisms is, polysaccharides, proteins, synthetic polymer, and the processing unit of the decomposition waste jellyfish of claim 1 which is formed in one of an inorganic material. Carrier fixing the microorganisms is, processor degradation effluent jellyfish according to any one of claims 1 to 2 having a porous structure. The processing apparatus of the jellyfish decomposition | disassembly waste liquid in any one of Claim 1 to 3 with which the microorganisms were fixed to the support | carrier by at least any one of the adhesion method, the crosslinking method, and the inclusion method. The jellyfish decomposition waste liquid according to any one of claims 1 to 4, wherein a carrier on which microorganisms are fixed is housed in a container, and the contact means is a liquid feeding device for feeding the jellyfish decomposition waste liquid into the container. Processing equipment. The treatment of the jellyfish decomposition waste liquid according to claim 5, wherein the liquid supply device includes a pipe for introducing the jellyfish decomposition waste liquid and a liquid supply pump for supplying the jellyfish decomposition waste liquid toward the carrier. apparatus. The processing apparatus of the jellyfish decomposition waste liquid in any one of Claim 5 to 6 with which the container was equipped with the dissolved oxygen concentration adjustment means which adjusts the dissolved oxygen concentration in the decomposition waste liquid of the jellyfish accommodated in an inside. The jellyfish decomposition waste liquid treatment apparatus according to any one of claims 1 to 7, further comprising a sterilization means for sterilizing the jellyfish decomposition waste liquid brought into contact with the carrier by the contact means. The apparatus for treating a jellyfish decomposition waste liquid according to any one of claims 1 to 8, further comprising a desalting means for performing a desalination treatment on the jellyfish decomposition waste liquid brought into contact with the carrier by the contact means. The jellyfish decomposition waste liquid treatment apparatus according to claim 9, wherein at least one of the sterilization means and the desalting means is a voltage application device that applies a voltage to the jellyfish decomposition waste liquid discharged from the container. The apparatus for treating a jellyfish decomposition waste liquid according to any one of claims 1 to 10, wherein the concentration of salt in the jellyfish decomposition waste liquid is 1% by mass or more. 12. The bacterium belonging to the genus Sphingobacterium is at least one selected from Sphingobacterium multivorum and Sphingobacterium spiritiborum. The processing apparatus of the decomposition waste liquid of the jellyfish in any one. Formed by bringing the jellyfish decomposition waste liquid decomposed with the prionase enzyme into contact with a carrier on which at least one of the microorganisms of the yeast and bacteria group is immobilized, and coagulating and precipitating the treatment liquid of the contacted jellyfish decomposition waste liquid A method for treating a jellyfish decomposition waste liquid that removes the aggregated precipitate,
The yeast is Rhodotorula mucilaginosa,
The bacteria group belongs to the genus Sphingobacterium, Pseudomonas rhizosphaerae, Pseudomonas flavesens, Pseudomonas flavescens, Pseudomonas flavums, A method for treating a jellyfish decomposition waste liquid , which is at least one selected from the group consisting of Bacillus pumilus . A microorganism characterized in that it is a Rhodotorula mucilaginosa 126-Aege strain (Accession No. FERM P-20896), which removes the COD component of the jellyfish decomposition waste liquid.