JP4834830B2 - レール分子固定方法及びナノ搬送デバイス - Google Patents
レール分子固定方法及びナノ搬送デバイス Download PDFInfo
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- JP4834830B2 JP4834830B2 JP2005135703A JP2005135703A JP4834830B2 JP 4834830 B2 JP4834830 B2 JP 4834830B2 JP 2005135703 A JP2005135703 A JP 2005135703A JP 2005135703 A JP2005135703 A JP 2005135703A JP 4834830 B2 JP4834830 B2 JP 4834830B2
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- Prior art keywords
- rail
- microtubule
- rail molecule
- molecule
- kinesin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- H—ELECTRICITY
- H02—GENERATION; CONVERSION OR DISTRIBUTION OF ELECTRIC POWER
- H02N—ELECTRIC MACHINES NOT OTHERWISE PROVIDED FOR
- H02N11/00—Generators or motors not provided for elsewhere; Alleged perpetua mobilia obtained by electric or magnetic means
- H02N11/006—Motors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B19/00—Machines or pumps having pertinent characteristics not provided for in, or of interest apart from, groups F04B1/00 - F04B17/00
- F04B19/006—Micropumps
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F05—INDEXING SCHEMES RELATING TO ENGINES OR PUMPS IN VARIOUS SUBCLASSES OF CLASSES F01-F04
- F05B—INDEXING SCHEME RELATING TO WIND, SPRING, WEIGHT, INERTIA OR LIKE MOTORS, TO MACHINES OR ENGINES FOR LIQUIDS COVERED BY SUBCLASSES F03B, F03D AND F03G
- F05B2250/00—Geometry
- F05B2250/80—Size or power range of the machines
- F05B2250/84—Nanomachines
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S977/00—Nanotechnology
- Y10S977/70—Nanostructure
- Y10S977/701—Integrated with dissimilar structures on a common substrate
- Y10S977/702—Integrated with dissimilar structures on a common substrate having biological material component
- Y10S977/705—Protein or peptide
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- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Nanotechnology (AREA)
- Mechanical Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
Vale, R.D., Reese, T.S. and sheetz, M.P., "Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility," Cell, vol. 42, pp. 39-50(1985) Howard, J., Hudspeth, A.J. and Vale, R., "Movement of microtubules by single kinesin molecules," Nature, vol. 342, pp. 154-159(1989) Vale, R.D., Funatsu, T., Pierce, D.W., Romberg, L., Harada, Y. and Yanagida, T., "Direct observation of single kinesin molecules moving along microtubules," Nature, vol. 380, pp. 451-453(1996) Svoboda, K., Schmidt, C., Schnapp, B, and Block, S., "Direct observation of kinesin stepping by optical trapping interferometry," Nature, vol.365, pp.721-727(1993) Nishiyama, M., Muto, E., Inoue, y., Yanagida, T. and Higuchi, H., "Substeps within the 8-nm step of the ATPase cycle of single kinesin molecules," Nat. Cell Biol., vol. 3, pp. 425-428(2001) Meyhofer, E. and Howard, J., "The force generated by a single kinesin molecule against an elastic load," Proc. Natl. Acad. Sci. U.S.A., vol. 92, pp.574-578(1995) Coppin, C.M., Pierce, D.W., Hsu, L. and Vale, R.D., "The load dependence of kinesin's mechanical cycle," Proc. Natl. Acad. Sci. U.S.A., vol. 94, pp.8539-8544(1997) Kojima, H., Muto, E., Higuchi, H. and Yanagida, T., "Mechanics of Single Kinesin Molecules Measured by Optical Trapping Nanometry," Biophys. J., vol. 73, pp. 2012-2022(1997)
(1)L42:波長が420〔nm〕以下の光をカットするUVカットフィルタ
(2)ロボン:波長が800〔nm〕以上の光をカットする赤外光カットフィルタ
(3)IF550:波長が500〜600〔nm〕の光を透過させるバンドパスフィルタ
(4)Y50:波長が500〔nm〕以下の光をカットするフィルタ
そして、図4(b)は、(1)〜(4)の4種類の光フィルタが透過する光の波長の関係を示している。なお、図4(b)において、横軸には波長を採ってある。
22 ガラス板
27 周辺凸部
28 中央凸部
29 ナノチャネル
31 微小管
32 キネシン
36 キネシンビーズ
Claims (10)
- (a)極性を備え、該極性に応じた方向に生体分子モータが移動するレール分子を固定する方法であって、
(b)生体分子モータ含有溶液によりコーティングすることによって基材上に生体分子モータを付着させ、
(c)レール分子を導入し、ATPを与えることにより、前記生体分子モータによってレール分子を移動させ、
(d)前記基材上に形成された配向用チャネル内に前記レール分子を進入させ、該レール分子が所定位置に到達すると、所定波長の光を照射して前記生体分子モータを失活させることにより、前記レール分子を所定方向に配向させて固定することを特徴とするレール分子固定方法。 - (a)前記基材上にチャネル形成部材を接合することによって、前記基材上に配向用チャネルを形成し、
(b)該配向用チャネルの一端から前記レール分子を前記配向用チャネル内に進入させ、
(c)該配向用チャネル内の所定位置に前記レール分子を固定する請求項1に記載のレール分子固定方法。 - 前記レール分子を固定した後、前記チャネル形成部材を除去する請求項2に記載のレール分子固定方法。
- 前記レール分子は細胞骨格繊維である請求項1に記載のレール分子固定方法。
- 前記所定波長は420〜500〔nm〕である請求項1に記載のレール分子固定方法。
- 前記光は60秒以上照射する請求項1に記載のレール分子固定方法。
- 複数本の前記レール分子を相互に平行に、かつ、同一方向に配向させて固定する請求項1に記載のレール分子固定方法。
- 前記レール分子を曲線又は折れ線を形成するように固定する請求項1に記載のレール分子固定方法。
- (a)請求項1〜8のいずれか1項に記載のレール分子固定方法によって固定されたレール分子と、
(b)該レール分子上を該レール分子の極性に応じた方向に移動する生体分子モータとを有し、
(c)該生体分子モータが前記レール分子に沿って搬送物を搬送することを特徴とするナノ搬送デバイス。 - 請求項1〜8のいずれか1項に記載のレール分子固定方法によって固定されたことを特徴とするナノ搬送デバイス用のレール分子。
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2005135703A JP4834830B2 (ja) | 2005-05-09 | 2005-05-09 | レール分子固定方法及びナノ搬送デバイス |
PCT/JP2005/021334 WO2006120774A1 (ja) | 2005-05-09 | 2005-11-21 | レール分子固定方法及びナノ搬送デバイス |
US11/919,640 US8088739B2 (en) | 2005-05-09 | 2005-11-21 | Method of fixing rail molecule and nano transport device |
US13/307,185 US20120070873A1 (en) | 2005-05-09 | 2011-11-30 | Method of fixing rail molecule and nano transport device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2005135703A JP4834830B2 (ja) | 2005-05-09 | 2005-05-09 | レール分子固定方法及びナノ搬送デバイス |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2006312211A JP2006312211A (ja) | 2006-11-16 |
JP4834830B2 true JP4834830B2 (ja) | 2011-12-14 |
Family
ID=37396295
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2005135703A Active JP4834830B2 (ja) | 2005-05-09 | 2005-05-09 | レール分子固定方法及びナノ搬送デバイス |
Country Status (3)
Country | Link |
---|---|
US (2) | US8088739B2 (ja) |
JP (1) | JP4834830B2 (ja) |
WO (1) | WO2006120774A1 (ja) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20140120631A1 (en) * | 2011-06-03 | 2014-05-01 | The Foundation For The Promotiom Of Industrial Science | Method of detecting protein and detecting device |
US9803231B2 (en) | 2011-12-29 | 2017-10-31 | Ibis Biosciences, Inc. | Macromolecule delivery to nanowells |
JP6487190B2 (ja) | 2014-11-21 | 2019-03-20 | サントル ナショナル ドゥラ ルシェルシュ シヤンティフィック | 分子検出システム |
GB201502866D0 (en) * | 2015-02-20 | 2015-04-08 | Drexler Kim E | Molecular motor |
JP6950522B2 (ja) * | 2017-12-27 | 2021-10-13 | ウシオ電機株式会社 | 微生物の不活化処理装置および細胞活性化処理装置、並びに微生物の不活化処理方法 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
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JP3341044B2 (ja) * | 2000-07-10 | 2002-11-05 | 独立行政法人産業技術総合研究所 | 微小駆動素子及びその製造方法 |
AU2003284274A1 (en) * | 2002-10-18 | 2004-05-04 | Florida State University | Biomolecular-based actuator |
JP4113950B2 (ja) * | 2003-08-13 | 2008-07-09 | 独立行政法人産業技術総合研究所 | 微粒子の片側のみをビオチン化した異方性ビチオン化ラテックス微粒子 |
-
2005
- 2005-05-09 JP JP2005135703A patent/JP4834830B2/ja active Active
- 2005-11-21 WO PCT/JP2005/021334 patent/WO2006120774A1/ja active Application Filing
- 2005-11-21 US US11/919,640 patent/US8088739B2/en not_active Expired - Fee Related
-
2011
- 2011-11-30 US US13/307,185 patent/US20120070873A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US8088739B2 (en) | 2012-01-03 |
US20100076180A1 (en) | 2010-03-25 |
JP2006312211A (ja) | 2006-11-16 |
US20120070873A1 (en) | 2012-03-22 |
WO2006120774A1 (ja) | 2006-11-16 |
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