JP4801030B2 - Inspection kit - Google Patents

Description

The present invention relates to a sample processing method and a test kit obtained by collecting a patient's nose, pharynx, conjunctiva, urethra, uterus, vagina, feces, urine, sputum, etc. with a sample collection jig. The present invention relates to a test kit for efficiently handling and processing a specimen that is desired to be processed without touching a handler.
In particular, the present invention relates to a test kit suitable for preparing a test sample for use in an immunoassay reagent.

  The test sample for immunoassay is immersed in an extract previously placed in a test container with biological samples such as tear fluid, eyelids, sputum, saliva, stool, gauze, cotton swabs, etc. The sample is then dispersed or dissolved to prepare an extract.

  By the way, as a test kit for performing a predetermined test on a specimen that is desired to be processed without touching the handler from a hygienic viewpoint, conventionally, for example, FIG. 9, FIG. 10, FIG. 11 (a), ( Test kits for testing stool toxins as shown in b) are used.

  As shown in FIG. 9, FIG. 10, FIG. 11 (a), (b), this test kit functions as a container for containing a sample and diluting with a diluent, and also has a lid on one side (ie, A filter 53 for filtering the diluted mixed solution 52 obtained by diluting the specimen with the diluent, is disposed in the cap / container 51 that also functions as a filter-side lid (described below) and the filter holding portion 55a on the one end side. On the other end side, a dropping tube 55 provided with a nozzle portion 54 for dropping the diluted mixed solution 52 filtered by the filter 53, and a nozzle side cap that functions as a lid on the nozzle portion 54 side of the dropping tube 55 56.

  When using this test kit, as shown in FIG. 10, as shown in FIG. 11 (a), the cap / container 51 contains the diluted mixed solution 52 in which the specimen is diluted with the diluted solution. In addition, the side of the dropping tube 55 on which the filter 53 is disposed is inserted into the opening of the cap / container 51, and further, as shown in FIG. The diluted mixed solution 52 is filtered by passing through the filter 53 and filled in the dropping tube 55.

  Thereafter, as shown in FIG. 12, the body portion 55 b of the dropping tube 55 is pressed with a finger to bend (bend), so that the filtered diluted mixed solution 52 in the dropping tube 55 is impregnated with the test solution. It is dropped on the plate 61 and the presence or absence (or some) of the toxin is inspected.

  By using the test kit as described above, the fecal toxin test can be performed efficiently.

  However, in the case of the conventional inspection kit, since the nozzle portion 54 of the dropping tube 55 is not sealed, the internal diluted mixed liquid 52 may leak out if the inspection kit is shaken or turned sideways. For this reason, it is difficult to sufficiently mix the diluted mixed liquid 52, and the extraction efficiency may be lowered, which may hinder the inspection and may cause a problem on hygiene.

  Further, as other conventional specimen storage containers, as shown in FIG. 13, a container main body 72 for storing a diluted specimen 71 containing influenza virus, and the diluted specimen 71 can be filtered and supplied onto a test device. A specimen storage container 76 including a filter 73 and a nozzle 74 that passes the diluted specimen 71 that has passed through the filter 73 and supplies the diluted specimen 71 to the test device 75 has been proposed (see Patent Document 1).

  However, even in the case of the specimen storage container 76 of Patent Document 1, since the nozzle 74 is not sealed, the diluted specimen 71 inside may leak out if the specimen storage container 76 is shaken or turned sideways. There is a problem that it is not preferable in terms of hygiene.

In addition, if the specimen contains highly infectious virus or microorganism, the test sample preparation method as described above may require secondary disposal such as nosocomial infections unless proper disposal management of used swabs and extracts is performed. May cause general infection spread.
International Publication No. 2004/081568 Pamphlet

The present invention solves the above-mentioned problems. For example, infectious bacteria and viruses such as highly infectious influenza viruses and adenoviruses, and tuberculosis and avian influenza viruses having a high risk of secondary infection. Provides test kits for safe and efficient handling and processing of specimens that are not desirable to be exposed to the environment or exposed to the external environment, such as specimens used in laboratory tests The purpose is to do.

In order to solve the above problems, the test kit of the present invention (Claim 1 ) is:
(a) a sample collection jig provided with a sample collection unit to collect a sample at least on one end side;
(b) An opening is provided on one end side, and a bottom having a thin-film portion is provided on the other end side opposite to the side on which the opening is provided, and the sample collection of the sample collection jig A container main body in which an extraction liquid to which the collected specimen is to be transferred is immersed;
(c) an opening sealing lid for sealing the opening of the container body;
(d) The sample collection part of the sample collection jig is immersed in the extract stored in the container body, the collected sample is transferred to the extract, and the sample collection jig is stored. by the opening sealing cover remains at the stage of the container body is sealed, the thin film portion of the bottom portion of the container main body, a through hole forming tip portion for forming a through hole for passing the extract And a step portion is provided between the thin portion on the distal end side and the thick portion on the proximal end side. An opening jig provided with a liquid passage disposed so as to allow the extracted liquid that has passed through the through-hole to be taken out to the outside, including the formed tip for forming the through-hole .
(e) A liquid that seals the liquid passage after taking out at least a part of the extract from the through-hole formed in the thin film portion of the container body to the outside through the liquid passage. And a passage sealing lid.

Further, the test kit of claim 2 is the configuration of the invention of claim 1 ,
Before immersing the specimen collection jig, an opening sealing lid for sealing the opening of the container body, and the specimen collection section after specimen collection are immersed in the extract to remove the specimen. In the stage after shifting to the extract, an opening sealing lid different from the opening sealing lid for sealing the opening of the container body is prepared,
As the opening sealing lid for sealing the opening of the container main body before the specimen is transferred to the extraction liquid, the extraction liquid is sealed by the container main body and the opening sealing lid. An extract sealing lid that can be sealed in the space is prepared,
As an opening sealing lid for sealing the opening of the container body at a stage after the sample is transferred to the extract, the sample collection jig is sealed together with the extract and the container body and the opening. A sealing lid with a specimen collecting jig accommodation function that can be sealed in a sealed space formed by a stopper is provided.

According to a third aspect of the present invention, there is provided the test kit according to the second aspect, wherein the sealing lid with the specimen collecting jig receiving function seals the specimen collecting jig in the sealed space without bending the specimen collecting jig. It is characterized by having dimensions that can be used.

Moreover, the test kit according to claim 4 is the structure of any one of claims 1 to 3 ,
(a) the bottom of the container body is disposed in a raised bottom at a position retracted from the tip of the other end of the container body;
(b) The opening jig is
A cylindrical portion that fits into an opening jig housing region from the other end side to the bottom on the inner peripheral side of the container main body so as to be in close contact with the inner peripheral surface of the container main body,
The through-hole-forming sharp tip disposed on one end of the cylindrical portion;
A liquid extraction passage communicating with an inner portion of the cylindrical portion, the end of which functions as an extraction liquid outlet, and a liquid passage sealing lid for sealing the liquid passage A liquid extraction flow path that constitutes the liquid passage together with an inner portion of the cylindrical portion that functions as a sealing lid attachment portion to be attached;
By pushing the unsealing jig into the unsealing jig housing area, the space between the unsealing jig and the container body is sealed, and a through-hole is formed in the thin film portion of the bottom of the container body. It is structured as follows.

According to a fifth aspect of the present invention, there is provided the inspection kit according to any one of the first to fourth aspects, wherein the liquid passage of the opening jig is provided with a filter for filtering and removing solid matter. It is characterized by being.

According to a sixth aspect of the present invention, there is provided the inspection kit according to any one of the first to fifth aspects of the present invention, wherein the through-hole forming point of the opening jig has a penetrating direction that penetrates the thin film-like portion. At least one slit that is substantially parallel is formed, and in a state where a through-hole is formed by breaking through the thin film-like portion of the bottom portion of the container body, the extract in the container body passes outside through the slit. It is configured to be taken out.

According to a seventh aspect of the present invention, there is provided the inspection kit according to any one of the first to sixth aspects, wherein the opening is provided in a side wall near the other end side region provided with the bottom of the container body. It is characterized by being more flexible than the side wall near the one end side region.

Also, test kit of claim 8, in the configuration of any one of the claims 1-7, wherein the specimen collecting jig is characterized by a cotton swab with a swab at least one end.

In addition, the test kit according to claim 9 is used for an infectious disease test.

The test kit according to claim 10 is used for testing for influenza virus.

The test kit of the present invention (Claim 1 ) includes (a) a sample collecting jig provided with a sample collecting part to collect a sample on at least one end side, and (b) an opening on one end side. A container main body having a bottom portion having a thin film-like portion on the end side, and containing an extraction liquid to which the sample is to be transferred by immersing the sample collecting portion of the sample collecting jig; and (c) an opening of the container main body. An opening sealing lid to be sealed; and (d) an extraction liquid in a thin film-like portion at the bottom of the container body at the stage where the container body is sealed with the opening sealing lid while the specimen collecting jig is accommodated. A through-hole forming point for forming a through-hole through which a distal end side is formed , and a distal end side thin portion and a proximal end side thick portion, the distal end side thin portion, a through hole forming tip portion a step portion is formed between the thickness of thick portion of the base end side, passes through the through hole An unsealing jig provided with a liquid passage for allowing the liquid to pass through and taking it out to the outside; and (e) a liquid for sealing the liquid passage after taking out at least a part of the extract through the liquid passage and providing it for inspection. Since it has a passage sealing lid, the sample should touch the handler, be exposed to the outside, or be scattered in the process from sample collection by the sample collection jig to disposal of the sample after the test. Can be prevented.

  In addition, the through-hole forming point includes a thin portion on the distal end side and a thick portion on the proximal end side, and a thin portion on the distal end side and a thick portion on the proximal end side Since the stepped portion is formed between them, the stepped portion comes into contact with the vicinity of the through-hole of the thin film-like portion at the bottom of the container body, and the thin-film-like portion is deformed. A gap is easily formed in the vicinity, and the extracted liquid in the container body can be more easily taken out to the outside through the gap.

Further, as in the test kit of claim 2 , in the configuration of the invention of claim 1 , an opening sealing lid for sealing the opening of the container body at a stage before the specimen collection jig is immersed; The opening sealing lid that is different from the opening sealing lid that seals the opening of the container body at the stage after the specimen collecting portion after specimen collection is immersed in the extraction liquid and the specimen is transferred to the extraction liquid As an opening sealing lid that seals the opening of the container body in the stage before the specimen is transferred to the extraction liquid, the extraction liquid is placed in a sealed space formed by the container body and the opening sealing lid. An extraction liquid sealing lid that can be sealed is prepared, and the specimen collection jig is used as an opening sealing lid that seals the opening of the container body at a stage after the specimen is transferred to the extraction liquid. Sample collection that can be sealed together with the extract in a sealed space formed by the container body and the opening sealing lid By preparing a sealing lid with a container storage function, it is possible to securely seal the specimen collection jig together with the extract in a sealed space, and handle specimens that should be tested for influenza virus. Therefore, it is possible to handle a sample that is likely to become a problem more hygienically and efficiently, and the present invention can be effectively realized.

Further, as in the test kit of claim 3 , in the configuration of the invention of claim 2 , as a sealing lid with a function of containing a specimen collecting jig, the specimen collecting jig is sealed in a sealed space without being bent. When a sample with a dimension that can be used is used, it is sealed in a sealed space formed by the container body and a sealing lid with a sample collecting jig accommodation function without deforming the sample collecting jig. It is possible to make the present invention more effective.

Further, as in the test kit according to claim 4 , in the configuration of any one of claims 1 to 3 , (a) the bottom of the container body is raised to a position retracted from the tip on the other end side of the container body. (B) a cylindrical portion that fits into the opening jig housing area on the inner peripheral side of the container body so as to be in close contact with the inner peripheral surface of the container body, and a cylindrical shape A tip for forming a through hole disposed on one end side of the portion, and a liquid extraction passage communicating with the inner portion of the cylindrical portion, the end portion serving as an extraction liquid outlet, and An opening jig comprising a liquid extraction flow path that constitutes a liquid passage together with an inner portion of the cylindrical portion, which functions as a sealing lid attachment portion to which a liquid passage sealing lid for sealing the liquid passage is attached. Is pressed between the opening jig and the container body, and the container body is sealed. When the through-hole is formed in the thin film-like portion of the bottom, the through-hole is formed in the thin-film-like portion of the bottom of the container body by pushing the opening jig into the opening jig housing area of the container main body. It becomes possible to seal between the opening jig and the container main body, and it is possible to ensure that the extract can be taken out only from the liquid extraction flow path, which makes the present invention more effective. It becomes possible.

Further, as in the test kit of claim 5 , in the configuration of any one of claims 1 to 4 , the liquid passage of the opening jig is provided with a filter for filtering and removing solid matter By doing so, it is possible to filter the extract containing the specimen and use it in a state in which no solid content is contained. That is, the test kit having the configuration of claim 8 is particularly significant when a specimen containing a solid content is handled.

Further, as in the inspection kit according to claim 6 , in the configuration of any one of the inventions according to claims 1 to 5 , the opening jig has a through hole forming pointed portion substantially at a penetrating direction penetrating the thin film portion. When at least one parallel slit is formed, when the through-hole is formed by piercing the thin film-like portion at the bottom of the container body with the through-hole forming point, the extract in the container body is passed through the slit. It can be reliably taken out to the outside, and the present invention can be more effectively presented.

Further, as the test kit of claim 7, in the configuration of any one of the claims 1 to 6, the side wall portion near the other end region with a bottom portion of the container main body, an opening portion is provided When configured to be more flexible than the side wall portion near the one end side region, the side wall portion near the other end side region is pressed so that the extraction liquid and the sample collecting portion of the sample collecting jig are sufficiently blended to increase the extraction efficiency. It is possible to improve, and when discharging the extract from the container body, the extract can be quickly discharged by pressing the side wall near the other end side region.

Further, in the configuration of the invention according to any one of claims 1 to 7 , as in the test kit according to claim 8 , a special sample is obtained by using a cotton swab having a cotton ball at least on one end side as a specimen collecting jig. The specimen can be collected without the need for a jig, and the present invention can be further effectively realized.
In the present invention, the sample collection jig is not limited to the above-described cotton swab, and uses a configuration having various sample collection units capable of collecting and holding a sample such as a brush on one end side. It is possible.

In addition, the test kit of the present invention is suitable for use in infectious disease tests like the test kit of claim 9 , and by using this test kit, infectious disease tests can be performed safely and efficiently. It becomes possible to carry out.

In addition, the test kit of the present invention is suitable for use in testing for influenza virus, as in the test kit of claim 10 , and by using this test kit, the test for influenza virus can be performed safely. Moreover, it can be carried out efficiently.

  The features of the present invention will be described in more detail below with reference to examples of the present invention.

  In Example 1, a test kit used for testing for influenza virus will be described as an example.

  FIG. 1 is an exploded view showing a configuration of a kit for testing influenza virus according to one embodiment of the present invention (a cross-sectional view of the main part), and FIG. 2 is a diagram showing an opening jig 14 that configures the kit for testing shown in FIG. (A) is a front sectional view and (b) is a side view.

  As shown in FIGS. 1 and 2, the test kit A of this embodiment includes (a) a sample collecting jig 2 provided with a sample collecting unit 1 on which one sample is to be collected, and (b) one end. An opening 3 on the side and a bottom 5 having a thin film portion 4 on the other end opposite to the side on which the opening 3 is provided. The sample collection unit 1 is immersed (see FIG. 3), and the container main body 6 that contains the extraction liquid to which the collected sample is to be transferred is contained, and (c) the opening 3 of the container main body 6 is sealed and extracted. An extraction liquid sealing lid 7a for sealing the liquid 8 in a sealed space formed by the container body 6 (opening sealing lid 7 used in a stage before the specimen is transferred to the extraction liquid 8); ) The sample collection part 1 of the sample collection jig 2 is immersed in the extract 8 contained in the container body 6 (see FIG. 3), and the collected sample is transferred to the extract 8. After that, the specimen collection jig 2 is sealed with a specimen collection jig accommodation function for sealing in the sealed space 9 (see FIGS. 4 and 5) formed by the container body 6 together with the extraction liquid 8 without bending the specimen collection jig 2. When the container body 6 is sealed by the lid 7b (the opening sealing lid 7 used in the stage after the specimen is transferred to the extract 8) and the sealing lid 7b with the specimen collecting jig accommodating function, the container The thin film portion 4 of the bottom portion 5 of the main body 6 is provided with a through hole forming point 12 for forming a through hole 11 (see FIGS. 6B and 6C) through which the extract 8 passes, and the through hole 11 An opening jig 14 provided with a liquid passage 13 disposed so that the extract 8 that has passed through can be taken out to the outside, and (e) formed in the thin film portion 4 of the container body 6. At least a part of the extract 8 is taken out from the through hole 11 through the liquid passage 13 and inspected. And a liquid passage sealing lid 15 for sealing the liquid passage 13 after being provided.

In this embodiment, a cotton swab provided with a cotton ball, which is the sample collection unit 1, on one end side is used as the sample collection jig 2 for collecting a sample.
In addition, as a cotton swab, you may use what formed the cotton ball by flocking synthetic fiber.
Furthermore, in the present invention, the specimen collecting jig is not limited to the above-described cotton swab, but has various configurations including a specimen collecting section capable of collecting and holding a specimen such as a brush on one end side. Can be used.

  Further, in this embodiment, except for the specimen collecting jig 2, each constituent member constituting the test kit is a transparent or translucent material having flexibility (in this embodiment, low density polyethylene). The amount of the contents can be visually confirmed, and after forming the through hole 11 (see FIGS. 6B and 6C), the container body 6 is pressed and deformed ( For example, the extraction liquid 8 can be dripped and supplied from the outlet 18 at the tip of the liquid passage 13 of the opening jig 14 by lightly pressing the body of the container body 6.

  In addition, an extraction liquid sealing lid 7a for sealing in a sealed space formed by the opening 3 of the container main body 6 and the container main body 6 (opening sealing used in a stage before the specimen is transferred to the extraction liquid 8). Screws are formed on the lid 7), and both are screwed together so that the opening 3 is sealed by the extract liquid sealing lid 7a.

  Further, a screw is also provided on the sealing lid 7b with a specimen collecting jig accommodating function (the opening sealing lid 7 used in a stage after the specimen is transferred to the extract 8) that seals the opening 3 of the container body 6. It is formed, both are screwed together, and the opening 3 is configured to be sealed by a sealing lid 7b with a specimen collecting jig housing function.

  Further, in the inspection kit A of this embodiment, the bottom 5 of the container body 6 is arranged in a raised bottom at a position retracted from the lower end of the container body 6. The opening jig 14 is a cylindrical part that fits into the opening jig housing area 16 that is an inner circumferential space below the bottom part 5 of the container body 6 so as to be in close contact with the inner circumferential surface of the container body 6. 17, the through hole forming point 12 disposed on one end side of the cylindrical portion 17, and the inner portion of the cylindrical portion 17, and the end portion functions as an extraction port 18 for the extract 8, And the liquid extraction flow path 19 that constitutes the liquid passage 13 together with the inner portion of the cylindrical portion that functions as a sealing lid attachment portion to which the liquid passage sealing lid 15 for sealing the liquid passage is attached. I have. Then, by pushing the opening jig 14 into the opening jig housing region 16, the gap between the opening jig 14 and the container body 6 is sealed, and the through-hole 11 is formed in the thin film portion 4 of the bottom 5 of the container body 6. Is formed.

  As a result, the opening jig 14 is pushed into the opening jig housing region 16 of the container body 6 to form the through-hole 11 in the thin film-like portion 4 of the bottom 5 of the container body 6, and the opening jig 14 and the container body 6 can be sealed, and the extract 8 can be reliably taken out only from the liquid extraction channel 19 without causing the extract 8 to leak from other portions.

Further, as shown in FIGS. 2 (a) and 2 (b), the extract 8 is also contained in the liquid passage 13 of the opening jig 14 even when the extract 8 contains a solid derived from the specimen. A filter 20 is provided for filtering and removing solid matter so that the solid matter can be filtered and used for inspection.
Note that the filter 20 may serve to reduce the viscosity of the extract from which the specimen is extracted.

  Further, a slit 21 (FIG. 2 (b), FIG. 6 (FIG. 2B), FIG. c)) is formed. Thereby, when the through-hole 11 is formed by piercing the thin-film-like portion 4 of the bottom portion 5 of the container body 6 with the through-hole forming point 12, the extract 8 in the container body 6 is passed through the slit 21 to the outside. It becomes possible to take it out reliably.

  Further, in order to make it easier to take out the extract 8 in the container body 6 to the outside more reliably, the tip 12 for forming the through hole of the opening jig 14 includes a thin portion 12a on the distal end side and a proximal end. The step portion 12c is formed between the thin portion 12a on the distal end side and the thick portion 12b on the proximal end side. In the case of such a configuration, the stepped portion 12c pushes the thin film-like portion 4 of the bottom 5 of the container body 6 to cause deformation, so that a gap is easily formed in the vicinity of the through hole 11, and the inside of the container body 6 It becomes easier to take out the extract 8 through the gap more reliably. That is, the thin film portion 4 of the bottom portion 5 is pierced by the thin portion 12a on the tip side, and the ruptured thin film portion 4 is surely pushed upward by the step portion 12c so that the extraction liquid is placed in the vicinity of the through hole 11. 8 can be reliably formed, and the extract 8 in the container body 6 can be reliably taken out through the passage.

  Next, a method for using the above-described test kit, that is, a method for processing a specimen using the test kit and testing for influenza virus will be described.

  (1) First, a sample (for example, a mucous membrane of the throat) is collected on one end side by a sample collecting jig 2 provided with a sample collecting unit 1 to collect a sample.

  (2) The extract-sealed lid 7a is removed from the opening 3 of the container body 6 having the bottom 5 having the thin film-like portion 4, and the extract 8 contained in the container body 6 as shown in FIG. The sample collection part 1 of the sample collection jig 2 is immersed, and the collected sample is transferred to the extract 8.

(3) Then, as shown in FIG. 4, the opening 3 of the container body 6 is sealed with a sealing lid 7 b having a specimen collection jig housing function, and the specimen collection jig 2 is extracted together with the extract 8 together with the container body. 6 and a sealed space 9 formed by a sealing lid 7b with a specimen collecting jig accommodating function.
In this state, the extract 8 is sealed in the sealed space 9, so that the sample and the extract can be sufficiently mixed by rocking or shaking the whole.

Moreover, in this invention, the side wall part 31 (FIG. 4) vicinity of the other end side area | region 31 (FIG. 4) provided with the bottom part 5 of the container main body 6 is a side wall vicinity 32 (FIG. 4) of the one end side area | region provided with the opening part 3. It is desirable to be more flexible than the part.
Specifically, the side wall of the container body 6 near the other end side region 31 provided with the bottom 5 can be deformed by pressing with a finger, and when the pressing force is removed, the original shape is restored. It is preferable to have a degree of flexibility.
As described above, as a method of making the side wall portion near the other end side region 31 provided with the bottom 5 of the container body 6 more flexible than the side wall portion near the one end side region 32 provided with the opening 3, for example, A method of making the thickness of the side wall portion near the other end side region 31 thinner than the thickness of the side wall portion near the one end side region 32 is exemplified.
In this case, the side wall portion near the other end side region 31 is pressed to squeeze the sample collecting part 1 of the sample collecting jig 2, and the extraction liquid 8 and the sample collecting part 1 are sufficiently adapted to increase the extraction efficiency. It becomes possible to improve and is preferable. Further, when the extract 8 is discharged from the container body 6, it is convenient to quickly discharge the extract 8 by pressing the side wall portion near the other end side region 31.

(4) Next, as shown in FIGS. 5, 6 (a), 6 (b), (c), the thin film-like portion 4 of the bottom 5 of the container body 6 is provided with a through-hole forming point 12. The opening jig 14 is fitted to the inner peripheral side of the bottom portion 5 of the container body 6 with the liquid passage sealing lid 15 attached, and further pushed into the bottom portion 5 to form a through hole of the opening jig 14. A through-hole 11 through which the extract 8 passes is formed in the thin film-like portion 4 of the bottom 5 by the pointed end portion 12 for use.
At this time, since the liquid passage sealing lid 15 is attached, the extract 8 does not leak to the outside even if the through hole 11 is formed.

  (5) Thereafter, as shown in FIG. 7, the liquid passage sealing lid 15 is removed, and at least a part of the extract 8 is removed from the through hole 11 formed in the thin film-like portion 4 of the container body 6 through the liquid passage. Take it out for inspection.

  At this time, since the slit 21 and the step portion 12c that are substantially parallel to the penetration direction penetrating the thin film portion 4 are formed in the through-hole forming point 12 of the opening jig 14, the through-hole forming point 12, when the through-hole 11 is formed by breaking through the thin film-like portion 4 of the bottom 5 of the container body 6, the step 12 c is formed on the bottom 5 of the container body 6 as shown in FIGS. 6 (b) and 6 (c). Since the thin film-like portion 4 is deformed by coming into contact with the vicinity of the through-hole of the thin film-like portion 4, a gap through which the extraction liquid 8 passes is easily formed in the vicinity of the through-hole 11 and the slit 21 is extracted. Since it becomes the flow path of the liquid 8, the extract 8 can be reliably taken out and can be used for inspection easily.

  (6) As described above, after the extraction liquid 8 is taken out, as shown in FIG. 8, the liquid passage is sealed with the liquid passage sealing lid 15 so that the extraction liquid 8 does not leak outside. The entire test kit can be discarded (for example, disposed in a dedicated disposal container or the like).

  This makes it possible to efficiently handle and process specimens that are not desirable to touch the handler from a hygiene perspective or to be exposed to the external environment, such as specimens used for influenza virus testing. Become.

  In the above embodiment, as the opening sealing lid 7, the extraction liquid sealing lid 7a (the opening sealing lid 7 used in the stage before the specimen is transferred to the extraction liquid 8) and the specimen collecting jig housing function The test kit provided with the attached sealing lid 7b (the opening sealing lid 7 used in the stage after the specimen is transferred to the extract 8) has been described as an example. 7, one opening sealing lid 7 in both stages before and after the specimen is transferred to the extract 8 (that is, both the function of sealing the extract and the function of accommodating the specimen collecting jig) It is also possible to use an opening sealing lid having the above function to reduce the number of parts.

  In addition, the liquid passage sealing lid 15 can be formed into a cap shape that covers the entire lower end portion of the opening jig 14, for example, and in this case, the extraction liquid after the sample extraction is opened. It is possible to more reliably cover the portion where the extract may adhere after being dropped from the liquid extraction passage 19 of the tool 14, which is preferable from the viewpoint of preventing infection.

  The invention of the present application is not limited to the above embodiment in other respects as well, the specific configuration of the specimen collection jig provided with the specimen collection section, the shape and structure of the container body and the opening sealing lid, and the opening treatment Various applications and modifications can be made within the scope of the invention with respect to the configuration of the tool, particularly the arrangement of the slits and stepped portions.

  As described above, according to the present invention, it is not desirable to touch the handler from the viewpoint of hygiene or to be exposed to the external environment, such as a specimen used for infectious bacteria or virus inspection. The specimen can be handled and processed safely and efficiently. Therefore, the present invention can be widely applied to the technical field of specimen processing and the field of test kits used therefor.

According to one embodiment of the present invention, a container body, an extraction liquid sealing lid that is an opening sealing lid, a sealing lid with a specimen collecting jig accommodating function, an opening jig, and a liquid passage sealing lid It is an exploded view (main part sectional view) of a configuration of a test kit provided. It is a figure which shows the opening jig | tool which comprises the test | inspection kit concerning one Example of this invention, (a) is front sectional drawing, (b) is a side view. It is a figure which shows one process of the method using the test kit concerning one Example of this invention, and immerses the sample collection part of a sample collection jig in an extract, and transfers the collected sample to an extract It is a figure which shows a process. It is a figure which shows 1 process of the method using the test kit concerning one Example of this invention, and immerses the sample collection part of a sample collection jig in an extract, The sealing lid with a sample collection jig accommodation function It is a figure which shows the state which covered the lid. It is a figure which shows 1 process of the method using the test kit concerning one Example of this invention, and is a figure which shows the process of forming a through-hole in the thin film-like part of the bottom part of a container main body. (a), (b), (c) is a figure which shows 1 process of the method using the test kit concerning one Example of this invention, and forms a through-hole in the thin film-like part of the bottom part of a container main body. It is a principal part enlarged view which shows a process. It is a figure which shows 1 process of the method using the kit for a test | inspection concerning one Example of this invention, and is a figure which shows the process of taking out extract liquid outside through a liquid passage from a through-hole, and using for a test | inspection. It is a figure which shows 1 process of the method using the test kit concerning one Example of this invention, and seal | sticks a liquid channel with a liquid channel sealing lid, and the whole test kit in the state where an extract does not leak outside It is a figure which shows the process of enabling to discard. It is a figure which shows the conventional container used as a test kit for the toxin test in a stool. It is a figure explaining the usage method of the conventional container shown in FIG. (a), (b) is a figure which shows 1 process of the method of using the conventional container shown in FIG. 9, respectively. It is a figure which shows the other process of the method of using the conventional container shown in FIG. It is a figure which shows the structure of the other conventional conventional container for container accommodation.

DESCRIPTION OF SYMBOLS 1 Specimen collection part 2 Specimen collection jig 3 Opening part 4 Thin film part 5 Bottom part 6 Container main body 7 Opening part sealing lid 7a Extraction liquid sealing lid 7b Sealing lid with specimen collection jig accommodation function 8 Extraction liquid 9 Sealed space DESCRIPTION OF SYMBOLS 11 Through-hole 12 Tip part for through-hole formation 12a Thin part at the front end side 12b Thick part at the base end side 12c Step part 13 Liquid passage 14 Opening jig 15 Liquid passage sealing lid 16 Opening jig accommodation area 17 Cylindrical portion 18 Extraction port 19 Liquid extraction flow path 20 Filter 21 Slit 31 Near the other end side region 32 Near the one end side region A Inspection kit

Claims (10)

(a) a sample collection jig provided with a sample collection unit to collect a sample at least on one end side;
(b) An opening is provided on one end side, and a bottom having a thin-film portion is provided on the other end side opposite to the side on which the opening is provided, and the sample collection of the sample collection jig A container main body in which an extraction liquid to which the collected specimen is to be transferred is immersed;
(c) an opening sealing lid for sealing the opening of the container body;
(d) The sample collection part of the sample collection jig is immersed in the extract stored in the container body, the collected sample is transferred to the extract, and the sample collection jig is stored. by the opening sealing cover remains at the stage of the container body is sealed, the thin film portion of the bottom portion of the container main body, a through hole forming tip portion for forming a through hole for passing the extract And a step portion is provided between the thin portion on the distal end side and the thick portion on the proximal end side. An opening jig provided with a liquid passage disposed so as to allow the extracted liquid that has passed through the through-hole to be taken out to the outside, including the formed tip for forming the through-hole .
(e) A liquid that seals the liquid passage after taking out at least a part of the extract from the through-hole formed in the thin film portion of the container body to the outside through the liquid passage. An inspection kit comprising a passage sealing lid. Before immersing the specimen collection jig, an opening sealing lid for sealing the opening of the container body, and the specimen collection section after specimen collection are immersed in the extract to remove the specimen. In the stage after shifting to the extract, an opening sealing lid different from the opening sealing lid for sealing the opening of the container body is prepared,
As the opening sealing lid for sealing the opening of the container main body before the specimen is transferred to the extraction liquid, the extraction liquid is sealed by the container main body and the opening sealing lid. An extract sealing lid that can be sealed in the space is prepared,
As an opening sealing lid for sealing the opening of the container body at a stage after the sample is transferred to the extract, the sample collection jig is sealed together with the extract and the container body and the opening. test kit of claim 1, wherein the specimen collecting tool housing function sealing lid that can be sealed in the sealed space formed by sealing the lid are provided. The specimen collecting tool housing function sealing lid is claim 2, wherein said those having a size capable of sealing to the enclosed space without bending the specimen collecting tool Inspection kit. (a) the bottom of the container body is disposed in a raised bottom at a position retracted from the tip of the other end of the container body;
(b) The opening jig is
A cylindrical portion that fits into an opening jig housing region from the other end side to the bottom on the inner peripheral side of the container main body so as to be in close contact with the inner peripheral surface of the container main body,
The through-hole-forming sharp tip disposed on one end of the cylindrical portion;
A liquid extraction passage communicating with an inner portion of the cylindrical portion, the end of which functions as an extraction liquid outlet, and a liquid passage sealing lid for sealing the liquid passage A liquid extraction flow path that constitutes the liquid passage together with an inner portion of the cylindrical portion that functions as a sealing lid attachment portion to be attached;
By pushing the unsealing jig into the unsealing jig housing area, the space between the unsealing jig and the container body is sealed, and a through-hole is formed in the thin film portion of the bottom of the container body. It is comprised as follows. The test | inspection kit in any one of Claims 1-3 characterized by the above-mentioned. The inspection kit according to claim 1 , wherein a filter for filtering and removing solid matter is disposed in the liquid passage of the opening jig. At least one slit substantially parallel to the penetrating direction penetrating the thin film portion is formed in the through hole forming point of the opening jig, and the thin film portion at the bottom portion of the container body is formed. The inspection according to any one of claims 1 to 5 , wherein the extraction liquid in the container body is taken out to the outside through the slit in a state where the through-hole is formed by breaking through. For kit. The side wall portion in the vicinity of the other end side region provided with the bottom of the container body is configured to be more flexible than the side wall portion in the vicinity of the one end side region in which the opening is provided . 6. The test kit according to any one of 6 . The test kit according to claim 1 , wherein the specimen collecting jig is a cotton swab provided with a cotton ball at least on one end side. The test kit according to claim 1 , wherein the test kit is used for an infectious disease test. The test kit according to claim 1 , wherein the test kit is used for a test for influenza virus.

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