JP4789904B2 - Urine test paper test rod - Google Patents

Description

  The present invention relates to a paper test stick used for human urinalysis, and in particular, a urinalysis paper test characterized in that an ink composition containing a urinalysis indicator is applied to the surface by printing. Regarding the stick.

  Conventionally, in finding, diagnosing, and treating diseases of domestic animals such as cats, dogs, and other pet animals, the state of the animals is exclusively observed. In addition, a test piece in which a filter paper impregnated with a test reagent is attached to a support for detection of glucose in urine is used as a test material for urine testing of domestic animals. For example, Patent Document 1 discloses an animal in which an ink composition containing a urine test indicator is attached and pulp fibers are formed in a piece or twisted shape to form an animal excrement disposal material. Urinalysis test materials are disclosed, in which case the urine test indicator is an indicator for detecting urine sugar, an indicator for detecting urine protein, an indicator for detecting urine pH, an indicator for detecting urine nitrogen-containing components, or an indicator for detecting urinary occult blood It is described that the ink composition contains a urinalysis indicator, a water-absorbing powder, and a binder. However, the test material for urinalysis is applied to domestic animals such as cats, dogs and other pet animals, and is not applicable to humans, and the pulp fiber to which the ink composition is attached is twisted. The test material formed in this way is used as an animal excretion treatment material, and is not directly contacted with urine when excreted into humans.

For humans, it is extremely important to quickly and easily know, for example, the state of urine fluid, the presence or absence of various components in the fluid, and the content thereof in the discovery, diagnosis, treatment, etc. of various diseases. For example, knowing the acid alkalinity (pH) of urine quickly and easily, and knowing the presence or absence of urine sugar, urine protein, urine nitrogen-containing components, urine occult blood, etc. This leads to diagnosis, early detection, prevention and treatment, and is extremely important.
Thus, the simple and rapid detection of the urine state and various components in the urine is extremely important in the discovery, diagnosis, treatment, etc. of various diseases. Various body fluid test bodies comprising a test body support and a reagent layer formed on the support are used.
In this type of body fluid test body, it is desired that the body fluid test body can be discharged and discarded in a flush toilet because the disposal of the body fluid test body after use is easy. Discloses a body fluid test body using a water dispersible base material as a support, and Patent Document 3 discloses a water dispersible base material used as a support for a body fluid test body. .
However, the body fluid test body uses paper or a plastic sheet as a support, and does not use a paper test rod.

Japanese Patent No. 2896021 Japanese Patent Laid-Open No. 60-238763 Japanese Patent Laid-Open No. 03-170570

  The present invention relates to a test specimen for urinalysis used for human urinalysis, and can easily contact urine with the test specimen without discoloring and becoming unusable even when stored in a high humidity environment. Urine test specimen made of urine test paper that can be discharged and discarded into a flush toilet after use without contaminating the fingertips with excreted urine and that can reduce the amount of the urine test indicator-containing ink composition. The purpose is to provide a bar.

  As a result of diligent research regarding urine test specimens used in human urinalysis, the present inventors have used paper test bars made of water-soluble or water-dispersible paper as urine test specimens. By using this test bar, an ink composition containing a urine test indicator is printed on the test bar without discoloring and becoming unusable even when stored in a high humidity environment compared to a plate-like support. To form an application part, and urine is brought into contact with the application part of the test rod for urinalysis, whereby the state of the urine fluid and the urine Detects the presence or absence of various components in it, and in particular, it is used for detecting human urine, and can be easily touched by picking with a finger when urinating and can be flushed after use. It is a paper test stick, and it is printed with a urine test indicator-containing ink composition by printing. By, whereas deposited by dipping, urinalysis test bars capable of reducing the adhesion significantly, and have completed the present invention found that the above problems can be solved.

That is, the present invention is as follows.
1. A urine test paper test bar for testing human urine, and an ink containing a urine test indicator on the surface and inside of a paper bar formed by twisting water-soluble or water-dispersible paper the compositions have a coating part coated by printing, urinalysis paper test bars, characterized in that printing on front of the base paper to form a rod-like body applied by the printing is twisted.
2. 1. The water-soluble or water-dispersible paper has a thickness of 10 to 90 g / m ² . A test stick made of urine test paper as described in 1.
3. 1. The paper stick-shaped application part is a part that leaves a range in which urine does not adhere to a finger when the stick is picked up with a finger and attached to urine. Or 2. The test stick made of urine test paper according to any one of the above.
4). 1. The urine test indicator is a urine sugar detection indicator, a urine protein detection indicator, a urine pH detection indicator, a urine nitrogen-containing component detection indicator, or a urine occult blood detection indicator . ~ 3. The test stick made of urine test paper according to any one of the above.
5. The above-mentioned 1. characterized in that the application by printing is application by planographic printing or relief printing . ~ 4. The test stick made of urine test paper according to any one of the above.
6). The application part is one of a horizontal stripe pattern, a vertical stripe pattern, and a mesh pattern . ~ 5. The test stick made of urine test paper according to any one of the above.
7). 5. The striped pattern described above, wherein the striped pattern is obtained by printing and applying an ink composition containing a urine test indicator with a different stripe . A test stick made of urine test paper as described in 1.

  According to the present invention, by using a paper test bar, it is possible to easily perform urinalysis of human excretion urine without discoloration and inability to use even when stored in a high humidity environment compared to a plate-like support. It is possible to reduce the amount of adhesion by printing and applying the urinalysis indicator-containing ink composition without polluting the fingertips for picking the test rod with excreted urine. It was possible to provide a test stick made of urine test paper.

Hereinafter, embodiments of the present invention will be described in detail.
First, the paper bar-like body constituting the support of the urine test paper test bar of the present invention will be described.
The paper rod-shaped body formed by twisting the water-soluble or water-dispersible paper forming the support of the urine test paper test bar of the present invention is the urine formed in the coating portion applied by printing on the support. During the color reaction between the ink composition containing the test indicator and a predetermined component in excreted urine and within the time required to determine the subsequent color change of the reagent layer, the portion where the urine is immersed dissolves. Although it does not disperse or disperse, the used one is drained to a flush toilet, that is, it does not dissolve or disperse in the presence of a small amount of water, but easily dissolves or disperses with a large amount of water. It is a paper rod-like body formed by twisting paper having properties, that is, a paper string shape made of paper having a so-called dissolving or dispersing property.

As described above, the requirements for the support of the urine test body are that it is easy to dispose of the urine test body after use. However, during the time required for determining the color change of the reagent layer during the color reaction between the ink composition applied to the support and a predetermined component in the urine and the subsequent urine, That is, the attached part does not dissolve or disperse.
When the paper rod-shaped body is brought into contact with excreted urine, it is necessary to pick one end with a finger and make contact with the excreted urine, so it is important to have a certain rigidity. is there.
Examples of water-dispersible substrates that form paper rods include water-soluble or water-dispersible paper. Examples of water-soluble or water-dispersible paper include recycled pulp and pulp that are water-soluble such as carboxymethylcellulose. Examples include paper made using a water-soluble resin as a binder, and paper made using a water-soluble resin such as carboxymethylcellulose as a binder is most preferable. Specifically, it is preferable that paper such as toilet paper is twisted to form a paper bar-like body like a paper string.

When the paper rod-shaped body is brought into contact with excreted urine, it is necessary to pick one end with a finger and bring it into contact with excreted urine, so it is important to have a certain rigidity. The thickness of the water-soluble or water-dispersible paper is not particularly limited, but is 10 to 90 g / m ² , preferably 15 to 70 g / m ² , particularly preferably 20 to 60 g / m ^2. . The paper thickness is usually expressed in weight (g / m ² ), and the actual thickness depends on the type of paper, but is 0.15 mm for 38 g / m ² paper and 25 g / m ² It was 0.1 mm. If the thickness is less than 10 g / m ² , wide paper is required to obtain the required rigidity, and if it exceeds 90 g / m ² , it becomes difficult to twist it into a rod-like body. In addition, in order to have both rigidity and printing characteristics, the ink-absorbing thin paper with excellent printing characteristics and the thick paper thicker than the rigid thin paper are overlapped, and the ink-absorbing thin paper is the surface of the paper rod It is also preferable to obtain a paper rod-like body by twisting as shown in FIG. As the ink-absorbing thin paper, one having a thickness of 3 to ²⁰ g / m ² , preferably 5 to 15 g / m ² , particularly preferably 7 to 10 g / m ² is used.

The paper bar obtained from the paper is discolored and unusable even when the test bar on which the bar-shaped body is used as a support and the indicator-containing ink composition for urinalysis is printed and stored in a high humidity environment. Can be saved without. The urinalysis paper test stick of the present invention is stored in a toilet because it is used for urinalysis, but since the toilet is generally provided adjacent to or adjacent to the bathroom, it is often in a high humidity environment. It is important that the storage stability under the high humidity environment is excellent. The excellent storage stability of the rod-shaped body in a high-humidity environment is that the volume of the rod-shaped body support relative to the surface area is significantly larger than that of a plate-shaped support. It is estimated that the large volume is due to absorbing moisture and minimizing the effect on the indicator-containing ink composition.
Further, the rod-like body needs to have a certain rigidity, and the size thereof is 3 to 20 cm in length, preferably 5 to 17 cm, more preferably 7 to 15 cm, and its diameter is 1 to 1 cm. It is 8 mm, preferably 2 to 7 mm, particularly preferably 3 to 6 mm. If the length is less than 3 cm, the fingertips may be soiled when adhering excreted urine, and if it exceeds 20 cm, handling is difficult and waste is increased. In addition, if the diameter is less than 1 mm, the volume with respect to the surface area is insufficient, moisture is not sufficiently absorbed, the storage stability in a high humidity environment is lowered, and the rigidity is insufficient so that it is difficult to add the ink composition to the print, resulting in discoloration. Judgment is difficult, and when the thickness exceeds 8 mm, the amount of the ink composition attached increases, which makes handling and storage difficult.
Furthermore, the application of the ink composition to the paper rod-shaped body of the present invention by printing may be performed on the entire surface of the rod-shaped body, but it is desirable to apply it to the portion left by hand.

Next, the ink composition of the present invention will be described.
The indicator for urinalysis contained in the ink composition of the present invention changes its color when it comes into contact with excreted urine, and qualitatively or quantitatively detects urine pH, urinary protein, glucose, occult blood, etc. Tetrabromphenol blue is used as a protein test for urine, and kurcose oxidase, veroxidase, 2.7-diaminofluorine dihydrochloride, N- (3-sulfoprovir is used as a test for glucose in urine. ) -3,3 ′, 5,5′-tetramethylbenzidine sodium, etc., as urine occult blood test agents, hydrogen peroxide cumene, tetramethylbenzine, etc., as urine urobilinogen test agents, Methanic acid, 3,4 methylenedioxybenzenediazonium tetrafluoroborate, etc. are urine ketone body test agents such as glycine, sodium nitroplumide And the like.

Examples of urine pH test agents include phenolphthalein, bromthymol blue, bromocresol purple, tetraiodophenolsulfonephthalein, brilliant yellow, phenol red, cresol red, and ethyl orange.
Furthermore, in order to obtain an ink composition containing these urine test indicators, these urine test indicators and cellulose and / or a derivative of the cellulose are dispersed or dissolved in an organic solvent to obtain an ink composition. To do. Specifically, cellulose and / or cellulose derivatives such as varnish, hydroxypropyl cellulose, etc. are added and dispersed or dissolved in an organic solvent such as methanol, ethanol, toluene, sodium hydroxide, etc. to obtain an ink composition. .

The ink composition may contain conventionally known additives such as a binder and a stabilizer.
For example, the ink composition for detecting urine pH will be specifically described. In the ink composition for detecting urine pH, an indicator composition comprising a pH indicator, a quaternary ammonium salt or an amine salt, a binder and a water-absorbing powder is contained in a solvent. However, the quaternary ammonium salt or amine salt may be excluded. The pH indicator can be used as long as the indicator changes in color tone according to the excretion urinary hydrogen ion concentration, and a wide range of pH regions can be measured by selecting and combining a plurality of indicators. For example, if methyl red and bromothymol blue are used in combination as a pH indicator, the pH can be determined in the range of pH 5-9.

When an appropriate amount of a quaternary ammonium salt or an amine salt is added to the indicator composition, the faded color with time is greatly suppressed, and a clear color can be obtained. As the quaternary ammonium salt, an alkyltrimethylammonium salt, an alkyldimethylbenzine ammonium salt, a sapamin type ammonium salt, or the like can be used. Of these, alkyldimethylbenzine ammonium salts are preferred. Further, a primary amine salt, secondary amine salt or polyethylene glycol which is a cationic surfactant can also be used.
Binders include sugarcane starch, potato starch, konjac flour, funari, agar, sodium alginate, trooaoi, tongaro gum, gum arabic, glue, levan, gelatin, casein, collagen and other natural hydrophilic polymer compounds, methylcellulose, hydroxypropyl Cellulose derivatives such as cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, semi-synthetic hydrophilic polymer compounds such as carboxymethyl starch and dialdehyde starch, polyvinyl alcohol, polyacrylamide, polyvinyl pyrrolidone or copolymers thereof, polyacrylic acid Synthetic polymer compounds such as sodium and polyethylene oxide are used.

As water-soluble polymer compounds having film formation, cellulose resins such as nitrocellulose, cellulose acetate, and ethyl cellulose, polyester resins, alkyd resins, polyurethane resins, epoxy resins, acrylic resins, vinyl chloride resins, epoxy resins, emulsions, etc. are used. It is done. Of these, urethane resin and polyvinyl butyral are preferred because they do not adversely affect the color reaction of the PH indicator.
The solvent is preferably a reagent, in particular, one that can dissolve or disperse the binder uniformly and stably. Specifically, non-solvents such as aromatic hydrocarbons, aliphatic hydrocarbons, esters, alcohols, etc. An aqueous solvent or water or a mixture thereof is used. In addition to the above components, a small amount of a wetting agent, for example, a nonionic surfactant, an anionic surfactant, a cationic surfactant, polyethylene glycol, or the like can be blended in the pH detection ink composition. . As the basic substance, ammonium hydroxide, alkali metal hydroxide, alkaline earth metal hydroxide or the like is used.
The water-absorbing powder promotes the contact between excreted urine and the pH indicator by being blended in the indicator composition, and promotes the color reaction of the pH indicator. As such a water-absorbing powder, those which are extremely acidic or alkaline when contacted with water are not suitable. Kaolin, synthetic silica, glass, cellulose block, calcium carbonate, aluminum silicate, etc. are used.

Furthermore, application by printing of the ink composition will be described.
In the present invention, it is necessary to apply the ink composition to the paper rod-like body by printing. 1A shows a urine test paper test bar of the present invention obtained by solid printing, and FIG. 1B shows a urine test paper test bar printed and applied in a horizontal stripe pattern.
Examples of printing on paper rods include printing means such as planographic printing, offset printing, intaglio printing, gravure printing, letterpress printing, letterpress printing, silk screen printing, brush coating printing, preferably brush coating printing, Lithographic printing and letterpress printing are preferred. FIG. 3 shows printing application of an ink composition using a brush coating type printing means, a planographic rotation type printing means, and a relief printing type printing means.

  Of course, the printing on the paper rod-shaped body is carried out by printing on the portion where the surface of the rod-shaped body is formed on the paper before the rod-shaped body is formed, or on the portion where various patterns are formed. It is good also as a rod-shaped body by twisting the paper which printed the thing. FIG. 1 (C) shows a urinalysis paper test bar of the present invention in which a mesh pattern is printed on a surface portion of a base paper on which a rod-shaped body is formed in advance, and then the base paper is twisted to form a rod-shaped body. The printed portion of the rod-shaped body may be provided with a printed portion on a part of the rod-shaped body, but a mesh pattern is applied to the entire surface of the base paper constituting the rod-shaped body, and then the base paper is twisted to form the rod-shaped body. A printing portion may be provided on the entire surface. That is, in the present invention, it is desirable to print by leaving a part to be picked by hand, but a printing part may be provided on the entire surface of the rod-like body without leaving a part to be picked, and in particular, a part such as a mesh pattern or a horizontal stripe pattern may be provided. When printing is performed, the entire surface of the rod-shaped body may be printed.

Conventionally, since the application of the urine test indicator-containing ink composition to the paper rod-like body was applied by dipping treatment, the ink composition penetrated into the inside of the rod-like body and required a large amount of ink composition. However, in the present invention, the ink composition is printed only on the surface of the rod-shaped body, or only the portion appearing on the surface in printing on the base paper-like body, or even on the entire surface, the pattern is printed. Since the adhesion of the ink composition is relatively small with respect to the immersion treatment, it is extremely preferable economically. FIG. 2 shows a urinalysis paper test bar obtained by conventional dip coating. In the rod-like body, the ink composition penetrated to the inside, and a large amount of the ink composition was consumed.
Further, when the ink composition is applied in a horizontal stripe shape or a spiral stripe shape, it is preferable to use an ink composition having a different urine test indicator for each striped pattern, and by using a different urine test indicator-containing ink composition. Multiple items of urine testing can be performed in a single operation.

Hereinafter, the present invention will be described more specifically with reference to examples, but the present invention is not limited to these examples.
[ Reference Example 1 ]
-Paper rod-like body Using a recycled paper with a basis weight of 38 g / m ² , a long paper roll with a width of 160 mm is twisted to obtain a paper rod-like body having a long paper string shape. The body was cut into a length of 10 cm to obtain a paper bar having a diameter of 4 mm and a length of 10 cm.
Ink composition for urine pH test The liquid for detecting urine pH was 17.6 ml of methyl red 20 mg / dl ethanol solution, 25 ml of 200 mg / dl ethanol solution of bromthymol blue, and 50 ml of 12 g / dl ethanol solution of polyvinylpyridone as a binder. And ethanol was added to make a total volume of 100 ml. To this pH detection solution, an alkyldimethylbenzine ammonium salt as a quaternary ammonium salt and polyvinyl alcohol as a film-forming water-soluble polymer compound were added to obtain an ink composition for urine pH test using ethanol as a solvent.

-Application of ink composition by printing The above-obtained urine pH detection ink composition was applied by lithographic printing to the 4 cm portion of one end of the paper bar obtained as described above, and a test bar made of urine pH test paper Got.
When the urine being excreted by a healthy person was brought into contact with the obtained urine pH test paper test rod, it could be used in the same manner as a normal test material.
-Preservability of paper test bar The obtained paper test bar was left in a room with a humidity of 77%, a room with a humidity of 60%, and a room with a humidity of 57% for 4 hours and 20 minutes. There was no change.
On the other hand, the following experiment was tried to evaluate the storage stability of the paper test bar when left in a bathroom with a humidity of 95%.

  That is, when a test paper printed on a piece of tissue paper and the test paper twisted into a rod-like body are stored in a bathroom with a humidity of 95%, the printing ink colored orange is In the case of the test paper, the whole color changed to yellow in 30 minutes, but in the case of the rod-shaped body, the color gradually changed from yellow to yellow, and 1/5 (2 cm) from both ends became yellow 20 minutes after storage. Strange, 2/5 yellowed in 40 minutes, 3/5 yellowed in 70 minutes, 4/5 yellowed in 100 minutes, 5/5 (10 cm) yellowed in 120 minutes, The whole thing turned yellow in the same 130 minutes. In other words, the test paper turned yellow in 30 minutes, but if the test paper was twisted to form a rod-like body, it took 130 minutes for the whole to turn yellow, and the rod-like body had better storage stability. I know that.

[ Reference Example 2 ]
In Reference Example 1, application of the ink composition by printing was carried out by letterpress printing, and the urine pH detection ink composition was applied with 4 mm wide stripes at 3 mm intervals to obtain a urine pH test paper test rod.
The obtained urine pH test paper test bar could be used in the same manner as in Reference Example 1.

[Example 3]
After applying the ink composition for urine pH test on the base paper before being twisted, printing it with a letterpress in a spiral lattice pattern at an equal interval of 1 mm in width and 5 mm, and then twisting to form a rod-like body, A test stick made of inspection paper was obtained.
The obtained urine pH test paper test bar could be used in the same manner as in Reference Example 1.

[Comparative Example 1]
In Reference Example 1, the ink composition was applied by dip coating, and the urine pH detection ink composition was applied to obtain a urine pH test paper test stick.
The obtained urine pH test paper test bar could be used in the same manner as in Reference Example 1, but was consumed by dip coating, and therefore consumed a larger amount of ink composition than print coating.

  The urinalysis paper test bar according to the present invention, when a human excretes urine, is picked with a finger and brought into contact with the urine, and by its discoloration reaction, the components in the urine are recognized, which is useful for diagnosis of diseases, etc. For the most part, it contributes to prevention and is used to improve health.

(A) shows the urine test paper test bar of the present invention obtained by solid printing, (b) shows the urine test paper test bar printed and applied in a horizontal stripe pattern, and (c) shows the surface of the rod-shaped body in advance. The urinalysis paper test bar of the present invention is shown in which a base paper portion is formed by printing, and then the base paper is twisted to form a rod-like body. A urine test paper test bar obtained by conventional dip coating is shown. The printing application of the ink composition using a brush coating type printing means, a planographic rotation type printing means, and a relief printing type printing means is shown.

Claims (7)

A urine test paper test bar for testing human urine, and an ink containing a urine test indicator on the surface and inside of a paper bar formed by twisting water-soluble or water-dispersible paper the compositions have a coating part coated by printing, urinalysis paper test bars, characterized in that printing on front of the base paper to form a rod-like body applied by the printing is twisted. Water-soluble or the thickness of the water-dispersible paper, urinalysis Paper test bars according to claim 1, characterized in that the 10~90g / m ^2. Coating portions of paper rod-shaped body is, any one of claims 1 or 2, characterized in that a portion of urine left range not adhere to the finger when to urine deposited picking rod-shaped body with a finger A test stick made of urine test paper as described in 1. Urine test indicator, urine sugar detection indicator, urine protein detection indicator, urine pH detection indicator, according to claim 1, wherein the urine nitrogen-containing component detection indicator or occult blood detection indicator The test stick made of urine test paper according to any one of the above. Is applied by printing, planographic printing, urinalysis Paper test bars according to claim 1, characterized in that the application by relief printing. Coating unit, horizontal stripes patterns, stripes, urinalysis Paper test bars according to claim 1, characterized in that any pattern of the mesh pattern. The test stick made of urine test paper according to claim 6 , wherein the striped pattern is obtained by printing and applying an ink composition containing an indicator for urine test with different stripes.

Images