JP3521435B2 - Glucose test ink composition and test sheet using the same - Google Patents
Glucose test ink composition and test sheet using the sameInfo
- Publication number
- JP3521435B2 JP3521435B2 JP05550193A JP5550193A JP3521435B2 JP 3521435 B2 JP3521435 B2 JP 3521435B2 JP 05550193 A JP05550193 A JP 05550193A JP 5550193 A JP5550193 A JP 5550193A JP 3521435 B2 JP3521435 B2 JP 3521435B2
- Authority
- JP
- Japan
- Prior art keywords
- glucose
- glucose test
- ink composition
- sheet
- antioxidant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、ブドウ糖混入溶液、特
に尿などの体液中のブドウ糖を検出する検査シートを形
成するのに適したインキ組成物及びそれを用いて形成さ
れた検査シートに関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an ink composition suitable for forming a test sheet for detecting glucose in a glucose-mixed solution, particularly body fluid such as urine, and a test sheet formed using the same. Is.
【０００２】[0002]
【従来の技術】尿などの体液中のブドウ糖量を簡便に検
出できるシートは、糖尿病の診断及び管理に有用であ
る。現在、ブドウ糖検査シートにおいて製造工程による
形態で分類すると以下の２つに大別される。
（ａ）含浸タイプ：ブドウ糖酸化酵素、ペルオキシダー
ゼ、被酸化性指示薬、結合剤からなる試薬組成物を水ま
たは水−アルコール系溶媒中に溶解または分散させる。
そして、この液に濾紙を含浸させ、乾燥後、濾紙をシー
トに貼着、断裁して作成されたブドウ糖検査シート。
（ｂ）印刷タイプ：ブドウ糖酸化酵素、ペルオキシダー
ゼ、被酸化性指示薬、結合剤からなる試薬組成物が溶媒
中に溶解あるいは分散されてなるインキを支持体上に印
刷してなるブドウ糖検査シート。2. Description of the Related Art A sheet capable of easily detecting the amount of glucose in a body fluid such as urine is useful for diagnosis and management of diabetes. Currently, glucose inspection sheets are roughly classified into the following two types according to the manufacturing process. (A) Impregnation type: A reagent composition comprising glucose oxidase, peroxidase, oxidizable indicator, and binder is dissolved or dispersed in water or a water-alcohol solvent.
Then, a glucose test sheet prepared by impregnating this solution with a filter paper, drying the filter paper, affixing the filter paper to the sheet, and cutting. (B) Printing type: A glucose test sheet obtained by printing on a support an ink in which a reagent composition comprising glucose oxidase, peroxidase, an oxidizable indicator and a binder is dissolved or dispersed in a solvent.
【０００３】上記（ａ）の含浸タイプのブドウ糖検査シ
ートは、製造工程が複雑であるため製造効率の低下によ
るコストの上昇を招いていた。これに対し（ｂ）の印刷
タイプのブドウ糖検査シートは含浸タイプにおける濾紙
の含浸、乾燥、貼着の工程を、インキの印刷、乾燥に置
き換え、製造工程の簡素化による大量生産、コストの低
下に優れている。The impregnated type glucose test sheet of (a) described above has a complicated manufacturing process, resulting in a decrease in manufacturing efficiency and an increase in cost. On the other hand, the printing type glucose test sheet of (b) replaces the impregnation, drying, and sticking process of the filter paper of the impregnating type with printing and drying of ink, which leads to mass production and cost reduction by simplifying the manufacturing process. Are better.
【０００４】これらのブドウ糖検査シートにおいて、被
酸化性指示薬はペルオキシダーゼにより酸化されて発色
する指示薬である。この被酸化性指示薬の例としはベン
ジジン類が広く知られている。このうち特にｏ−トリジ
ンが反応性の優位さから広く用いられている。そして、
ブドウ糖が存在すると酵素の酸化作用により、ｏ−トリ
ジンは青い呈色を示す。In these glucose test sheets, the oxidizable indicator is an indicator which is oxidized by peroxidase to develop a color. Benzidines are widely known as examples of the oxidizable indicator. Of these, o-tolidine is particularly widely used because of its superior reactivity. And
In the presence of glucose, o-tolidine shows a blue color due to the oxidative action of the enzyme.
【０００５】しかしながら、このｏ−トリジンを用いた
ブドウ糖検査シートは、大気中に放置しておくと除々に
黄褐色に変色してくる。その結果、上記のごとく青く呈
色するｏ−トリジンの色調に変化を生じさせ、さらに言
えば黄褐色の程度により呈色の再現性を阻害する。ブド
ウ糖検査シートは体液中の糖を診断及び管理するもので
あり、このような再現性の阻害は誤判定を下す原因につ
ながる。However, the glucose test sheet using this o-tolidine gradually turns yellow-brown when left in the atmosphere. As a result, the color tone of o-tolidine, which is colored blue as described above, is changed, and more specifically, the degree of yellowish brown hinders the reproducibility of coloration. The glucose test sheet is for diagnosing and managing sugar in body fluid, and such inhibition of reproducibility leads to erroneous determination.
【０００６】前述したブドウ糖検査シートの黄褐色化の
原因は、大気中の酸化物が被酸化性指示薬に作用する事
に起因すると考えられ、問題点として改善されるべきも
のである。 この様な背景に対する解決策として、従
来、被酸化性指示薬の安定化を促す目的で抗酸化剤が添
加されている。この抗酸化剤として顕著に用いられてい
る例としてはトコフェロール類が挙げられる。トコフェ
ロール類は常温にて液性を示す。The cause of the above-mentioned yellowish brown color of the glucose test sheet is considered to be due to the fact that the oxide in the air acts on the oxidizable indicator, and it should be improved as a problem. As a solution to such a background, an antioxidant is conventionally added for the purpose of promoting stabilization of the oxidizable indicator. Examples of prominently used antioxidants include tocopherols. Tocopherols are liquid at room temperature.
【０００７】そのためブドウ糖検査用インキ中では溶液
として溶解あるいは分散している。しかし前記製造方法
において、ブドウ糖検査用インキを支持体上に塗布した
後、乾燥させる際、液性であるトコフェロール類が残留
する可能性がある。こうような状況が起きた場合、被酸
化性指示薬の抗酸化剤として添加しているトコフェロー
ル類の残留液成分が、逆に被酸化性指示薬の酸化反応の
場として働くという欠点を有していた。Therefore, it is dissolved or dispersed as a solution in the glucose test ink. However, in the above-mentioned manufacturing method, when the glucose test ink is applied onto the support and then dried, liquid tocopherols may remain. When such a situation occurs, the residual liquid component of the tocopherols added as an antioxidant of the oxidizable indicator has the drawback that it acts as a place for the oxidation reaction of the oxidizable indicator. .
【０００８】[0008]
【発明が解決しようとする課題】本発明は、液性抗酸化
剤が乾燥後も検査シート中に残留し、逆に被酸化性指示
薬の酸化反応を促すという問題点に着目してなされたも
ので、その課題とするところは、ブドウ糖検査シート中
の液性を完全に除去し、大気中に放置しても酸化作用に
よる変色のないブドウ糖検査シートを提供することにあ
る。DISCLOSURE OF THE INVENTION The present invention has been made by focusing on the problem that the liquid antioxidant remains in the inspection sheet even after drying, and conversely promotes the oxidation reaction of the oxidizable indicator. Then, the problem is to provide a glucose test sheet that completely removes the liquid property in the glucose test sheet and is not discolored due to the oxidation action even when left in the atmosphere.
【０００９】[0009]
【課題を解決するための手段】本発明は、上述の課題を
解決するためになされたものであって、被酸化性指示
薬、結合剤、吸水性粉末、抗酸化剤およびブドウ糖酸化
酵素、ペルオキシダーゼからなるブドウ糖検査用インキ
において、前記抗酸化剤にフラボノイド系抗酸化剤を用
いるブドウ糖検査用インキ組成物である。The present invention has been made to solve the above-mentioned problems, and comprises an oxidizable indicator, a binder, a water-absorbing powder, an antioxidant and glucose oxidase, and a peroxidase. In the glucose test ink, the ink composition for glucose test uses a flavonoid antioxidant as the antioxidant.
【００１０】また、上記のブドウ糖検査用インキ組成物
を、支持体上に塗布してなるブドウ糖検出機能を有する
ブドウ糖検査シートである。A glucose test sheet having a glucose detection function, which is obtained by applying the above-mentioned glucose test ink composition onto a support.
【００１１】言い換えると、ブドウ糖検査用インキ組成
物において、被酸化性指示薬の抗酸化剤として非液性つ
まり粉末であるフラボノイド系を添加し、そのインキ組
成物を支持体上に印刷してブドウ糖検査シートを形成す
るものである。In other words, in a glucose test ink composition, a non-liquid or powdered flavonoid system is added as an antioxidant of an oxidizable indicator, and the ink composition is printed on a support to perform a glucose test. It forms a sheet.
【００１２】本発明において用いられるフラボノイド系
抗酸化剤とは、具体的にはヘスペリジン、ヘスペリチ
ン、ケルセチンのようなものが挙げられる。Specific examples of the flavonoid antioxidants used in the present invention include hesperidin, hesperitin, and quercetin.
【００１３】[0013]
【作用】本発明は、被酸化性指示薬の抗酸化剤として非
液性つまり粉末であるフラボノイド系抗酸化剤を用いる
ので、ブドウ糖検査用インキ組成物の支持体上への印
刷、乾燥後においても粉末であるので、安定であり、大
気中の酸化物による被酸化性指示薬の酸化作用から起こ
るブドウ糖検査シートの黄褐色化を抑制することができ
る。In the present invention, since a non-liquid, that is, a flavonoid antioxidant that is a powder is used as an antioxidant of an oxidizable indicator, even after printing the glucose test ink composition on a support and drying. Since it is a powder, it is stable, and it is possible to suppress the yellowish brown coloration of the glucose test sheet caused by the oxidizing action of the oxidizable indicator by the oxides in the air.
【００１４】[0014]
【実施例】本発明を、実施例を挙げて詳細に説明する。The present invention will be described in detail with reference to examples.
【００１５】＜実施例１＞下記組成のブドウ糖検査用イ
ンキ組成物を乳鉢で微細化および分散化して調製した。Example 1 A glucose test ink composition having the following composition was prepared by pulverizing and dispersing in a mortar.
【００１６】 （ブドウ糖検査用インキ組成物） クエン酸 ０．３０重量部 クエン酸ナトリウム １．１０重量部 ドデシル硫酸ナトリウム ０．５０重量部 ヘスペレチン ０．４０重量部 n-ペンチルアルコール ２６．５５重量部 ポリビニルアセタール樹脂 １．８５重量部 o-トリジン ０．７５重量部 微結晶セルロース １４．６２重量部 ブドウ糖酸化酵素 ０．５０重量部 ペルオキシダーゼ ０．４１重量部[0016] (Glucose test ink composition) Citric acid 0.30 parts by weight Sodium citrate 1.10 parts by weight Sodium dodecyl sulfate 0.50 part by weight Hesperetin 0.40 parts by weight n-pentyl alcohol 26.55 parts by weight Polyvinyl acetal resin 1.85 parts by weight o-Tolysine 0.75 parts by weight Microcrystalline cellulose 14.62 parts by weight Glucose oxidase 0.50 part by weight Peroxidase 0.41 parts by weight
【００１７】以上の組成物からなるブドウ糖検査用イン
キを、スクリーン印刷により白色ポリエチレンシート上
に５φとなるよう印刷した。スクリーン版は１５０メッ
シュ、ゾル厚８０μｍのものを用いた。得られた印刷物
を６０℃で３０分間乾燥後、スティックに断裁してブド
ウ糖検査シートを作製した。得られたブドウ糖検査シー
トを０〜５００ｍｇ／ｄｌのブドウ糖濃度である尿中に
浸漬したところ、尿中のブドウ糖濃度が高くなるにつれ
濃厚な青い呈色を示し、この色調変化による定量性は十
分であった。The glucose test ink comprising the above composition was printed on a white polyethylene sheet by screen printing so as to have a diameter of 5φ. The screen plate used was 150 mesh and had a sol thickness of 80 μm. The obtained printed matter was dried at 60 ° C. for 30 minutes and then cut into a stick to prepare a glucose test sheet. When the obtained glucose test sheet was immersed in urine having a glucose concentration of 0 to 500 mg / dl, it showed a deep blue coloration as the glucose concentration in the urine increased, and the quantification due to this color tone change was not sufficient. there were.
【００１８】＜比較例＞実施例１のブドウ糖検査用イン
キ組成物において、ヘスペレチンの代わりに従来技術の
抗酸化剤であるＤＬα−トコフェロールを添加して実施
例１と同様にしてブドウ糖検査シートを作製した。実施
例１と比較例１のブドウ糖検査シートを大気中に数日間
放置して評価したところ、実施例１で得られた検査シー
トの方が色調の変化が少なかった。結果を（表１）に示
す。Comparative Example A glucose test sheet was prepared in the same manner as in Example 1, except that DLα-tocopherol, which is a conventional antioxidant, was added to the glucose test ink composition of Example 1 in place of hesperetin. did. When the glucose test sheets of Example 1 and Comparative Example 1 were left to stand in the air for several days and evaluated, the test sheet obtained in Example 1 showed less change in color tone. The results are shown in (Table 1).
【００１９】[0019]
【表１】 [Table 1]
【００２０】上記（表１）において、測定器はミノルタ
カメラ（株）製 色彩式差計ＣＲ−２４１を用いた。表
において、Ｈ：色相、Ｖ：明度、Ｃ：彩度、ΔＥ：Ｌａ
ｂ表色系における色差、すなわち、Ｌ、ａ、ｂ空間にお
ける２点間の直線距離を示す。In the above (Table 1), a color difference meter CR-241 manufactured by Minolta Camera Co., Ltd. was used as a measuring instrument. In the table, H: hue, V: lightness, C: saturation, ΔE: La
The color difference in the b color system, that is, the linear distance between two points in the L, a, and b spaces is shown.
【００２１】[0021]
【発明の効果】本発明に係わるフラボノイド系化合物
は、ブドウ糖検査シートにおいて抗酸化剤として働き、
前記化合物自身が非液性なので、従来技術と比べてブド
ウ糖検査用インキを印刷し、乾燥した後も安定であり、
検出部の色調変化を抑える効果を奏する。The flavonoid compound according to the present invention functions as an antioxidant in a glucose test sheet,
Since the compound itself is non-liquid, it is more stable after printing the glucose test ink than in the prior art and drying it.
The effect of suppressing the change in color tone of the detection unit is exerted.
【００２２】従って、ブドウ検査シートの呈色安定性が
向上し、使用有効期間が延びる。Therefore, the coloration stability of the grape inspection sheet is improved and the effective period of use is extended.
───────────────────────────────────────────────────── フロントページの続き (56)参考文献 特開 平５−7499（ＪＰ，Ａ) 特開 昭47−5748（ＪＰ，Ａ) 特開 昭60−178356（ＪＰ，Ａ) 特開 昭60−178358（ＪＰ，Ａ) 特開 平５−76396（ＪＰ，Ａ) (58)調査した分野(Int.Cl.7，ＤＢ名) C12Q 1/00 - 3/00 G01N 31/22 ＢＩＯＳＩＳ／ＭＥＤＬＩＮＥ／ＷＰＩＤ Ｓ（ＳＴＮ) ＪＩＣＳＴファイル（ＪＯＩＳ)─────────────────────────────────────────────────── ─── Continuation of the front page (56) Reference JP-A-5-7499 (JP, A) JP-A-47-5748 (JP, A) JP-A-60-178356 (JP, A) JP-A-60- 178358 (JP, A) JP-A-5-76396 (JP, A) (58) Fields investigated (Int.Cl. 7 , DB name) C12Q 1/00-3/00 G01N 31/22 BIOSIS / MEDLINE / WPID S (STN) JISST file (JOIS)
Claims (2)
酸化剤およびブドウ糖酸化酵素、ペルオキシダーゼから
なる糖検査用インキにおいて、前記抗酸化剤にフラボノ
イド系抗酸化剤を用いることを特徴とするブドウ糖検査
用インキ組成物。1. A sugar test ink comprising an oxidizable indicator, a binder, a water-absorbing powder, an antioxidant, a glucose oxidase, and a peroxidase, wherein a flavonoid antioxidant is used as the antioxidant. A glucose test ink composition.
物を、支持体上に塗布してなるブドウ糖検出機能を有す
るブドウ糖検査シート。2. A glucose test sheet having a glucose detection function, which is obtained by applying the glucose test ink composition of claim 1 on a support.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP05550193A JP3521435B2 (en) | 1993-03-16 | 1993-03-16 | Glucose test ink composition and test sheet using the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP05550193A JP3521435B2 (en) | 1993-03-16 | 1993-03-16 | Glucose test ink composition and test sheet using the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH06261794A JPH06261794A (en) | 1994-09-20 |
| JP3521435B2 true JP3521435B2 (en) | 2004-04-19 |
Family
ID=13000408
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP05550193A Expired - Fee Related JP3521435B2 (en) | 1993-03-16 | 1993-03-16 | Glucose test ink composition and test sheet using the same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3521435B2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4552096B2 (en) * | 2000-08-29 | 2010-09-29 | 東洋紡績株式会社 | Colored paper and method for producing the same |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0653075B2 (en) * | 1984-02-24 | 1994-07-20 | 大日本印刷株式会社 | Glucose detection ink composition and test body formed using the same |
| JPH0653074B2 (en) * | 1984-02-24 | 1994-07-20 | 大日本印刷株式会社 | Body fluid test body |
| JPH057499A (en) * | 1991-07-02 | 1993-01-19 | Dainippon Printing Co Ltd | Printing ink composition for highly sensitively detecting glucose for otc, production thereof and application thereof |
| JPH0576396A (en) * | 1991-09-18 | 1993-03-30 | Terumo Corp | Stabilized material for color identification test and tool for color identification test |
-
1993
- 1993-03-16 JP JP05550193A patent/JP3521435B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH06261794A (en) | 1994-09-20 |
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