JP4773750B2 - Dispensing device, dispensing method and analyzer - Google Patents

Description

  The present invention relates to a dispensing device, a dispensing method, and an analyzer for dispensing a liquid by a syringe having a piston performing a liquid suction / discharge operation.

  2. Description of the Related Art Conventionally, a dispensing device that drives a piston to suck a liquid into a syringe and discharges the liquid from the syringe is known. In general, this piston is connected to a rotary motor via a rotation-linear motion converting mechanism such as a ball screw, and the rotational motion of this rotary motor is converted into a linear motion to perform a liquid suction / discharge operation. The dispensing apparatus controls the liquid dispensing operation by controlling the rotation of the rotary motor.

  By the way, when performing minute dispensing, this piston is used to reliably discharge a desired amount of liquid mass from the nozzle tip against the viscosity, surface tension, etc. of the liquid dispensed from the nozzle tip connected to the syringe. It is necessary to drive at high acceleration / deceleration and high speed. However, as described above, since the piston is driven via a rotary motor, a rotation-linear motion conversion mechanism, or the like, the predetermined drive speed may not be reached within a predetermined time. FIGS. 6A and 6B are schematic diagrams illustrating a drive profile that is a change in the drive speed (V) with respect to the drive time (t) of the piston. A broken line shows an ideal driving speed profile.

  As shown in FIG. 6A, when the piston driving speed V1 that has reached the predetermined time t0 is too low with respect to the predetermined driving speed Vth, the dispensed liquid 15B that does not detach from the nozzle 13 remains, and therefore the container 16 The dispensing liquid 15 </ b> C discharged to the amount is too small with respect to a predetermined dispensing amount.

  Also, as shown in FIG. 6B, when the piston drive speed does not reach the predetermined drive speed Vth within the predetermined time t0, the dispensed liquid 15D does not leave the tip of the nozzle 13 at all, and the container 16 Not dispensed. When performing minute dispensing, this piston needs to be driven along a drive profile that reaches a predetermined drive speed Vth within a predetermined time t0. That is, it is difficult to determine whether or not the dispensing is effective only with the piston driving distance. Therefore, a technique is disclosed in which a droplet ejected from the nozzle tip is imaged and whether or not dispensing is effective based on the image of the captured droplet (see Patent Document 1).

Japanese Patent Laid-Open No. 7-333231

  However, since the technique described above requires an image pickup device that picks up an image of a droplet and an image analysis device that analyzes an image of the picked-up liquid droplet, the dispensing device is complicated and enlarged, and the manufacturing cost is reduced. There was a problem of making it up.

  Further, when the shape of the liquid droplets ejected from the nozzle is not uniform, it is difficult to analyze the dispensing amount, and there is a problem that it cannot be reliably determined whether or not the dispensing is effective.

  The present invention has been made in view of the above, and an object of the present invention is to provide a dispensing device, a dispensing method, and an analyzer that can reliably determine whether dispensing is effective with a simple configuration. To do.

In order to achieve the above-mentioned object, a dispensing apparatus according to claim 1 is provided in a dispensing apparatus in which a syringe having a piston performs a liquid suction / discharge operation and dispenses the liquid. Storage means for storing a reference drive profile serving as a reference for the drive position and drive time, position detection means for detecting the execution drive position of the driven piston, and time counting for measuring the execution drive time of the driven piston And a drive control of the piston based on the reference drive profile and an execution drive profile obtained based on the execution drive position and the execution drive time of the piston, the reference drive profile and the execution drive Drive control means for comparing the profile and determining the suitability of the dispensing based on the comparison value;
It is provided with.

  In the dispensing device according to claim 2, in the above invention, the reference drive profile includes the drive time of the piston, the drive distance of the piston, and a predetermined time after the piston starts driving. It includes at least one of a driving speed of the piston in which a position, the driving position, and the driving time are associated with each other.

  According to a third aspect of the present invention, in the above-described dispensing device, the reference drive profile has a movement distance of the piston until the piston driving speed becomes zero for the first time after the piston starts driving. It is within a predetermined range.

  According to a fourth aspect of the present invention, in the above invention, the reference drive profile requires the piston to move before the piston drive speed becomes zero for the first time after the piston starts driving. The moving speed including acceleration / deceleration of the piston within a time or a range in which the piston has moved is within a predetermined range.

According to a fifth aspect of the present invention, there is provided a dispensing method according to the above invention, wherein a syringe having a piston performs a suction / discharge operation of a liquid to dispense the liquid in the dispensing method in which the piston is activated. A storage step for storing a reference drive profile serving as a reference of a position and a drive time, a position detection step for detecting an execution drive position of the driven piston, and a timing step for measuring the execution drive time of the driven piston And performing drive control of the piston based on the reference drive profile and obtaining an execution drive profile based on the execution drive position and the execution drive time of the piston, and the reference drive profile and the execution drive profile And a drive control step for determining the appropriateness of the dispensing based on the comparison value,
It is characterized by including.

  According to a sixth aspect of the present invention, in the above invention, the analyzer includes the dispensing device according to any one of the first to fourth aspects.

  The dispensing apparatus, the dispensing method, and the analyzing apparatus according to the present invention compare the reference drive profile of the piston that performs dispensing properly with the execution speed profile at which the dispensing is performed, thereby performing the dispense that has been performed. There is an effect that it is possible to reliably determine whether or not it is effective.

  Exemplary embodiments of a dispensing device, a dispensing method, and an analyzer according to the present invention will be described below in detail with reference to the accompanying drawings.

(Embodiment)
FIG. 1 is a block diagram showing a schematic configuration of a dispensing apparatus 1 according to the present invention. As shown in FIG. 1, the dispensing device 1 includes a control unit 2 that controls the dispensing operation, a storage unit 3 that stores a drive start position, a drive stop position, and a reference drive profile of the piston 10, and a liquid 15 The input unit 4 for inputting the viscosity and the dispensing amount, the display unit 17 for displaying whether or not the dispensing is effective, and the motor for controlling the motor 8 by inputting the control signal S1 from the control unit 2 A driver 5, a motor 8 driven by the motor driver 5, a position detector 7 that detects the position of the piston 10 based on the rotational position of the motor 8, and a position detected by the position detector 7 is converted into a detection signal S2. A signal converter 6 that performs conversion, a ball screw 9 that converts the rotational motion of the motor 8 into linear motion, a piston 10 that is coupled to the ball screw 9, a syringe 11 that houses the piston 10, and a reservoir 14 that houses liquid 15. , A three-way valve 12 for switching the suction and discharge by inputting the selection signal S3 from the control unit 2, and a nozzle 13 for discharging liquid 15 to the container 16.

  The control unit 2 includes a timer 20 that measures the driving time of the piston 10 and a comparison unit 21 that compares the reference drive profile stored in the storage unit 3 with the executed drive profile.

  Here, the dispensing operation of the dispensing apparatus 1 will be described. First, when the control unit 2 inputs the viscosity and the dispensing amount related to the liquid 15 via the input unit 4, the control unit 2 extracts a table corresponding to the viscosity and the dispensing amount from the storage unit 3.

  The control unit 2 outputs a selection signal S3 to the three-way valve 12, and switches the suction port of the three-way valve 12 to the reservoir 14 side. Thereafter, the control unit 2 outputs a control signal S1, moves the piston 10 to the drive start position described in the table, and sucks the liquid 15 from the reservoir.

  Next, the control unit 2 outputs a selection signal S3 to the three-way valve 12, switches the suction port of the three-way valve 12 to the syringe 11 side, outputs a control signal S1, and based on the reference drive profile described in the table The piston 10 is driven. This reference drive profile is set so that the movement distance of the piston 10 is within a predetermined range until the drive speed of the piston 10 becomes zero for the first time after the movement of the piston 10 starts. Furthermore, the time required from the start of the movement of the piston 10 until the speed becomes zero, or the movement speed including acceleration / deceleration of the piston 10 during that time is set to be within a predetermined range.

  The control unit 2 drives the piston 10 based on the reference drive profile and generates an execution drive profile of the piston 10 based on the timer 20 and the detection signal S2, and the comparison unit 21 generates the generated execution drive. Compare the profile with the reference drive profile.

  When the execution drive profile is within a predetermined range with respect to the reference drive profile based on the result of comparison by the comparison unit 21, the control unit 2 determines that the dispensing is valid, and the display unit 17 performs the dispensing. If the execution drive profile is outside the predetermined range with respect to the reference drive profile, it is determined that the dispense is invalid, and the display unit 17 displays that the dispense is invalid.

  Here, a table stored in the storage unit 3 will be described. FIG. 2 is a schematic diagram showing specific contents of the tables T1 to Tn. As shown in FIG. 2, each table T1 to Tn is described separately for each liquid viscosity, and a reference drive profile including a drive start position, a drive stop position, and a reference speed profile corresponding to the dispensing amount. Is described.

  The drive start position indicates the position with respect to the syringe 11 where the piston 10 starts driving, the drive stop position indicates the position with respect to the syringe 11 where the piston 10 stops driving, and the reference speed profile is driven from the start of driving of the piston 10. The speed profile until stop is shown.

  In these tables T1 to Tn, a reference drive profile in which the inner diameter of the syringe 11 is φ0.2 mm is described. For example, the table T1 shows a drive start position, a drive stop position, and a reference drive profile for dispensing the liquid 15 having a viscosity of 1.0 (mPa · s). When dispensing 90 (nL), the drive start position of the piston 10: 5, the drive stop position: 7.86, and the speed profile: A are shown. This indicates that 90 (nL) can be dispensed when the piston 10 is driven from the syringe 11 position: 5 to the syringe 11 position: 7.86 by the speed profile: A.

  In addition, when the liquid 15 is dispensed by 10 (nL), the drive start position: 1, the drive stop position: 1.32, and the speed profile: B of the piston 10 are shown. This indicates that 10 (nL) can be dispensed when the piston 10 is driven from the syringe position 1 to the syringe 11 position: 1.32 by the speed profile: B.

  Thus, the reference drive profile of the piston 10 varies depending on the dispensing amount. In addition, depending on the positional relationship of the syringe 11 with respect to the dispensing device 1, there is a case where the drive start position is prioritized and a case where the drive stop position is prioritized, both the drive start position and the drive stop position are described.

  Here, the speed profile will be described. FIG. 3 is a schematic diagram showing velocity profiles: A and B. As shown in FIG. 3, the speed profile: A is for 90 (nL) dispensing, and the speed profile: B is for 10 (nL) dispensing. In order to completely separate the liquid mass of the liquid 15 from the tip of the nozzle 13 in accordance with the dispensing amount, the speed profiles A and B have different accelerations.

  An operation procedure for determining whether the dispensing operation is valid will be described. FIG. 4 is a flowchart showing the dispensing operation. As shown in FIG. 4, first, the control unit 2 inputs the viscosity and dispensing amount of the liquid 15 (step S101). Next, the control unit 2 extracts a table corresponding to the viscosity and the dispensing amount input from the storage unit 3 (S102). Thereafter, the piston 10 is moved to the drive start position described in the extracted table (step S103), and the piston 10 is driven based on the reference drive profile described in the table (step S104).

  The control unit 2 acquires the execution drive profile that is actually executed based on the time measured by the timer 20 and the detection signal S2 (step S105). Therefore, the comparison unit 21 compares the execution drive profile with the reference drive profile (step S106). The control unit 2 determines whether or not the execution drive profile is within a predetermined range with respect to the reference drive profile (step S107). When it is determined that the execution drive profile is within the predetermined range (step S107, YES), dispensing is performed. Is determined to be valid (step S108), and the dispensing operation is terminated (step S109).

  On the other hand, when it is determined that the execution drive profile is not within the predetermined range with respect to the reference drive profile (step S107, NO), it is determined that the dispensing is invalid (step S110), and the fact that the dispensing is invalid is displayed (step S110). Step S111).

  When it is determined that the dispensing is invalid, the control unit 2 may discard the dispensing liquid or the entire container 16. Moreover, you may make it perform dispensing again. The linear motion-rotation conversion mechanism of the dispensing device according to the present invention may employ a rack and pinion mechanism instead of the ball screw 9 shown in FIG. Further, a linear motor may be used in place of the rotary motor 8 so that a linear motion-rotation conversion mechanism such as the ball screw 9 is unnecessary. Further, in the embodiment of FIG. 1, the quality determination result of the dispensing is displayed on the display unit 17, but the display unit 17 is not provided, and for example, when invalid dispensing is performed, the control unit 2 You may transmit an error signal to the control part of the analyzer which mounts the dispensing apparatus 1 by invention.

  In addition, the reference drive profile includes the drive start position, the drive stop position, and the reference drive profile of the piston 10 corresponding to the dispensing amount, but any one of these may be included, The comparison unit 21 may compare the execution drive profile corresponding to the reference drive profile.

  Next, an analyzer equipped with this dispensing device will be described. FIG. 5 is an upper surface of the analyzer 30 provided with the dispensing devices 1a and 1b. As shown in FIG. 5, this analyzer 30 has a work table 31. The work table 31 is a reaction table that mixes a specimen table 32 that contains a specimen, a reagent table 39 that contains a reagent, and a reagent and a specimen. 34, and also has dispensing devices 1a and 1b for dispensing reagents and specimens, respectively.

  In addition, the work table 31 includes a reading device 41 that individually identifies the reagent containers 40 stored in the reagent table 39, a light source 36 that irradiates analysis light to the reaction container 35 in which the reagent and the sample are mixed, and a reaction container. A light receiving element 37 for receiving the light transmitted through 35, and a cleaning device 38 for cleaning the reaction vessel 35 that has been analyzed.

  The dispensing apparatus 1a aspirates the sample from the sample container 33 accommodated in the accommodation chamber 32a of the sample table 32, and discharges the sample into the reaction container 35 accommodated in the accommodation chamber 34a of the reaction table 34, so that the dispensing apparatus 1b. Draws the reagent from the reagent container 40 accommodated in the accommodation chamber 39 a of the reagent table 39 and discharges the reagent into the reaction container 35.

  These two dispensing devices 1a and 1b individually dispense the sample and the reagent, respectively, and know the suitability of the dispensing individually, so that it is not necessary to perform a wasteful analysis and an erroneous analysis. Never do.

  In this embodiment, by comparing the reference drive profile stored in the storage unit 3 and the executed execution drive profile, it can be reliably determined whether or not the dispensing is effective. In addition, a reaction vessel supply and discharge device may be provided instead of the cleaning device 38 in FIG. 5, and in this case, the reaction vessel that has been analyzed does not stay in the device but is discharged out of the analysis device 30. The

  In this embodiment, the storage unit 3 creates the tables T1 to Tn for each viscosity of the liquid 15. However, the storage unit 3 does not divide each of the viscosities and describes all in one table. Good. Further, the predetermined range of the execution speed profile with respect to the reference speed profile may be changed according to the dispensing amount.

  The reference drive profile may be created when the dispensing apparatus 1 is shipped from the factory, when the apparatus is installed, when maintenance is performed, and when the apparatus is repaired. Further, it may be periodically updated so as to cope with a change with time. The reference speed profile may be created by actually performing a dispensing operation, or may be input to the storage unit 3 by simulation calculation or the like.

  Further, in this embodiment, the storage unit 3 stores the drive start position, the drive stop position, and the reference drive profile of the piston 10 together. However, any one of them is stored as a comparison unit. 21 may compare any one of the reference drive profile and the execution drive profile to determine whether dispensing is appropriate.

It is a block diagram which shows schematic structure of the dispensing apparatus concerning embodiment of this invention. It is a schematic surface which shows the content of the table which the memory | storage part concerning embodiment of this invention memorize | stores. It is a schematic diagram which shows the reference | standard speed profile concerning embodiment of this invention. It is a flowchart which shows the dispensing operation | movement concerning embodiment of this invention. It is a top view which shows schematic structure of the analyzer concerning embodiment of this invention. It is a schematic diagram which shows the case where the drive speed of a piston is too low. It is a schematic diagram which shows the case where the drive acceleration of a piston is too small.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Dispensing device 2 Control part 3 Memory | storage part 4 Input part 5 Motor driver 6 Signal converter 7 Position detector 8 Motor 9 Ball screw 10 Piston 11 Syringe 12 Three-way valve 13 Nozzle 14 Reservoir 15 Liquid 16 Container 17 Display part 20 Timer 21 Comparison Unit 22 Analyzing unit 30 Analyzing device 31 Work table 32 Sample table 32a, 34a, 39a Storage chamber 33 Sample container 34 Reaction table 35 Reaction vessel 36 Light source 37 Light receiving element 38 Washing device 40 Reagent container 41 Reading device

Claims (6)

In a dispensing device that performs a suction and discharge operation of a liquid having a piston and dispenses the liquid,
Storage means for storing a reference drive profile as a reference of a drive position and a drive time when starting the piston;
Position detecting means for detecting an execution drive position of the driven piston;
A time measuring means for measuring an execution drive time of the driven piston;
The drive control of the piston is performed based on the reference drive profile, the execution drive profile is obtained based on the execution drive position and the execution drive time of the piston, and the reference drive profile and the execution drive profile are obtained. A drive control means for comparing and judging whether the dispensing is appropriate based on the comparison value;
A dispensing device characterized by comprising:   The reference drive profile includes the drive time of the piston, the drive distance of the piston, a position after a predetermined time has elapsed since the piston started driving, and the piston corresponding to the drive position and the drive time. The dispensing apparatus according to claim 1, comprising at least one of driving speeds.   3. The reference drive profile according to claim 1, wherein the movement distance of the piston is within a predetermined range until the drive speed of the piston becomes zero for the first time after the start of driving of the piston. The dispensing device described.   The reference drive profile includes a time required for the piston to move or acceleration / deceleration of the piston within a range in which the piston moves until the piston driving speed becomes zero for the first time after the piston starts driving. The dispensing apparatus according to claim 3, wherein the moving speed is within a predetermined range. In a dispensing method in which a syringe having a piston performs a liquid suction and discharge operation to dispense the liquid,
A storage step of storing a reference drive profile that is a reference of a drive position and a drive time when starting the piston;
A position detection step of detecting an execution drive position of the driven piston;
A time measuring step for measuring an execution drive time of the driven piston;
The drive control of the piston is performed based on the reference drive profile, the execution drive profile is obtained based on the execution drive position and the execution drive time of the piston, and the reference drive profile and the execution drive profile are obtained. A drive control step of comparing and judging whether the dispensing is appropriate based on the comparison value;
A dispensing method characterized by comprising:   An analyzer comprising the dispensing device according to any one of claims 1 to 4.

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