JP4768534B2 - Containerized green tea beverage - Google Patents

Containerized green tea beverage Download PDF

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JP4768534B2
JP4768534B2 JP2006189319A JP2006189319A JP4768534B2 JP 4768534 B2 JP4768534 B2 JP 4768534B2 JP 2006189319 A JP2006189319 A JP 2006189319A JP 2006189319 A JP2006189319 A JP 2006189319A JP 4768534 B2 JP4768534 B2 JP 4768534B2
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green tea
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glutamic acid
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JP2008011834A (en
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紀子 古市
枝里 板屋
宏和 高橋
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Kao Corp
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本発明は非重合体カテキン類を高濃度に含有し、かつ苦味及び渋味を抑制させた容器詰緑茶飲料に関する。   The present invention relates to a packaged green tea beverage containing a high concentration of non-polymer catechins and suppressing bitterness and astringency.

カテキン類の作用としては、コレステロール上昇抑制作用やα−アミラーゼ活性阻害作用などが報告されている。このような生理効果を発現させるためには、より簡便に大量のカテキン類を摂取するために、飲料にカテキン類を高濃度配合する技術が望まれていた。   As the action of catechins, an action to suppress cholesterol elevation and an action to inhibit α-amylase activity have been reported. In order to express such a physiological effect, in order to ingest a large amount of catechins more easily, a technique for blending catechins with a high concentration in a beverage has been desired.

カテキン類を高濃度に含む飲料は、飲んだときに苦味・渋味が強く感じられ、常飲が困難である。これら茶系飲料の苦味・渋味を低減する方法として、サイクロデキストリンを配合する方法(例えば特許文献1〜3)、テアニンを含有する「包種茶」を配合する方法(例えば特許文献4)、グルタミン酸を配合する方法(例えば特許文献5)が報告されている。
すなわち、特許文献1は、茶抽出物1質量部乾燥質量に対し、サイクロデキストリン2.5質量部以上を含有する茶抽出物含有組成物並びに同含有食品を、特許文献2は、カテキン類1質量%、カフェイン0.1質量%以下及びサイクロデキストリン0.1〜20質量%の各量を含む飲食物の製造に際し、茶抽出液に水蒸気賦活炭を作用させカフェインを吸着・除去する方法を、特許文献3は、カテキン及びサイクロデキストリンを各特定量含む容器詰飲料を開示している。
また、特許文献4は、通常の烏龍茶よりも発酵度の低い「包種茶」を50%以上配合することにより、テアニンの含有量を所定以上に設定し、茶の苦味や渋味を抑制する方法を、特許文献5は、カテキン及びグルタミン酸を各特定量含む茶系飲料を開示している。
特開平3−168046号公報 特開平10−4919号公報 特開2002−238518号公報 特開2000−197449号公報 特開2006−42728号公報
Beverages containing a high concentration of catechins are strongly bitter and astringent when ingested and are difficult to drink regularly. As a method of reducing the bitterness and astringency of these tea-based beverages, a method of blending cyclodextrin (for example, Patent Documents 1 to 3), a method of blending “packed tea” containing theanine (for example, Patent Document 4), A method for blending glutamic acid (for example, Patent Document 5) has been reported.
That is, Patent Document 1 describes a tea extract-containing composition and food containing 2.5 parts by mass or more of cyclodextrin with respect to 1 part by mass of tea extract, and Patent Document 2 discloses 1 mass of catechins. %, Caffeine 0.1 mass% or less and cyclodextrin 0.1-20 mass%, in the production of food and drink, a method of adsorbing and removing caffeine by acting steam activated charcoal on tea extract Patent Document 3 discloses a packaged beverage containing specific amounts of catechin and cyclodextrin.
In addition, Patent Document 4 sets the content of theanine to a predetermined level or more by blending 50% or more of “baked tea” having a lower degree of fermentation than ordinary oolong tea to suppress the bitterness and astringency of tea. Regarding the method, Patent Document 5 discloses a tea-based beverage containing specific amounts of catechin and glutamic acid.
JP-A-3-16846 Japanese Patent Laid-Open No. 10-4919 JP 2002-238518 A JP 2000-197449 A JP 2006-42728 A

しかし、非重合体カテキン類を高濃度に含む容器詰飲料に、サイクロデキストリンを配合することによって加熱殺菌処理後の苦味・渋味を低減させるべく検討したところ、多量のサイクロデキストリンが必要であった。また、多量のサイクロデキストリンを配合するとサイクロデキストリン自身の風味によって飲料本来の風味が損なわれてしまう欠点があり、使用量については自ずと限界が生じることが判明した。
また、非重合体カテキン類を高濃度に含む容器詰飲料に、テアニン又はグルタミン酸を配合することによって加熱殺菌処理後の苦味・渋味を低減させるべく検討したところ、多量のテアニン又はグルタミン酸が必要であった。多量のテアニン又はグルタミン酸を配合するとテアニン又はグルタミン酸自身の風味によって緑茶本来の風味が損なわれてしまう欠点があり、使用量については自ずと限界が生じることが判明した。
従って、本発明の目的は、飲料本来の風味を損うことなく、非重合体カテキン類を高濃度に含む容器詰飲料の苦味や渋味を低減する手段を提供することにある。
However, a study was conducted to reduce bitterness and astringency after heat sterilization by adding cyclodextrin to a packaged beverage containing a high concentration of non-polymer catechins, and a large amount of cyclodextrin was required. . Further, it has been found that when a large amount of cyclodextrin is blended, the original flavor of the beverage is impaired by the flavor of the cyclodextrin itself, and the amount used is naturally limited.
In addition, when we studied to reduce the bitterness and astringency after heat sterilization by adding theanine or glutamic acid to a packaged beverage containing a high concentration of non-polymer catechins, a large amount of theanine or glutamic acid was required. there were. It has been found that when a large amount of theanine or glutamic acid is added, the original flavor of green tea is impaired by the flavor of theanine or glutamic acid itself, and the amount used is naturally limited.
Accordingly, an object of the present invention is to provide a means for reducing the bitterness and astringency of a packaged beverage containing a high concentration of non-polymer catechins without impairing the original flavor of the beverage.

そこで本発明者は、非重合体カテキン類を高濃度に含む容器詰飲料の加熱殺菌後の苦味及び渋味を、風味を低下させることなく、低減させるべく種々検討した結果、テアニン、グルタミン酸又はその塩、サイクロデキストリンは、それぞれ単独では十分な効果が得られないが、これらの成分を特定量で含有させることにより、相乗的に作用して苦味及び渋味低減効果が得られ、飲料本来の風味を保持した容器詰飲料が得られることを見出した。   Therefore, the present inventors have conducted various studies to reduce the bitterness and astringency after heat sterilization of a packaged beverage containing a high concentration of non-polymer catechins without reducing the flavor, and as a result, theanine, glutamic acid or its Salt and cyclodextrin are not able to obtain sufficient effects by themselves, but by containing these components in specific amounts, synergistic effects can be obtained to reduce bitterness and astringency, resulting in the original flavor of the beverage. It was found that a container-packed beverage retaining the above can be obtained.

すなわち、本発明は、次の成分(A)〜(C):
(A)非重合体カテキン類 0.1〜0.5質量%、
(B)テアニン 0.003〜0.03質量%、
(C)グルタミン酸又はその塩 0.0008〜0.005質量%、
を含有し、
(C)/(B)が0.27以下で、かつ((B)+(C)×30)/(A)が0.3以上である容器詰緑茶飲料を提供するものである。
That is, the present invention includes the following components (A) to (C):
(A) Non-polymer catechins 0.1 to 0.5% by mass,
(B) Theanine 0.003-0.03% by mass,
(C) glutamic acid or a salt thereof 0.0008 to 0.005 mass%,
Containing
A container-packed green tea beverage in which (C) / (B) is 0.27 or less and ((B) + (C) × 30) / (A) is 0.3 or more is provided.

本発明によれば、非重合体カテキン類を高濃度で含む容器詰飲料であって、苦味、渋味が抑制され、かつ風味が良好な飲料が提供できる。   ADVANTAGE OF THE INVENTION According to this invention, it is a container-packed drink which contains non-polymer catechins by high concentration, Comprising: A bitter taste and astringency are suppressed and a drink with favorable flavor can be provided.

本発明における非重合体カテキン類とは、カテキン、ガロカテキン、カテキンガレート、ガロカテキンガレートなどの非エピ体カテキン類及びエピカテキン、エピガロカテキン、エピカテキンガレート、エピガロカテキンガレート等のエピ体カテキン類である。ここでいう非重合体カテキン類の濃度は、上記の合計8種の合計量に基づいて定義される。   Non-polymer catechins in the present invention are non-epimeric catechins such as catechin, gallocatechin, catechin gallate and gallocatechin gallate and epi-catechins such as epicatechin, epigallocatechin, epicatechin gallate and epigallocatechin gallate It is. The density | concentration of non-polymer catechin here is defined based on said total amount of 8 types.

本発明の容器詰飲料中には、水に溶解状態にある(A)非重合体カテキン類を、好ましくは0.1〜0.5質量%、好ましくは0.1〜0.4質量%、好ましくは0.11〜0.4質量%、好ましくは0.11〜0.3質量%、より好ましくは0.11〜0.2質量%含有する。非重合体カテキン類含量が0.1質量%未満である場合、体内に吸収される十分な摂取量ではなく、0.5質量%を超えると飲料の苦味が増加する。   In the packaged beverage of the present invention, (A) non-polymer catechins dissolved in water are preferably 0.1 to 0.5% by mass, preferably 0.1 to 0.4% by mass, Preferably it contains 0.11-0.4 mass%, Preferably it is 0.11-0.3 mass%, More preferably, it contains 0.11-0.2 mass%. When the content of non-polymer catechins is less than 0.1% by mass, it is not a sufficient intake amount absorbed into the body, and when it exceeds 0.5% by mass, the bitterness of the beverage increases.

本発明の容器詰飲料中の(A)非重合体カテキン類は、エピガロカテキンガレート、ガロカテキンガレート、エピカテキンガレート、カテキンガレートからなる(D)非重合体カテキンガレート体類と、エピガロカテキン、ガロカテキン、エピカテキン、カテキンからなる非ガレート体類に分類される。本発明容器詰飲料中の(A)非重合体カテキン類中の(D)非重合体カテキンガレート体類の割合((D)/(A):質量比)は0.3〜0.6、更に0.4〜0.6であることが非重合体カテキン類の苦味抑制の観点から好ましい。   (A) Non-polymer catechins in the packaged beverage of the present invention are epigallocatechin gallate, gallocatechin gallate, epicatechin gallate, catechin gallate (D) non-polymer catechin gallate bodies, and epigallocatechin , Gallocatechin, epicatechin, and catechins are classified into non-gallate compounds. The ratio ((D) / (A): mass ratio) of (D) non-polymer catechin gallates in (A) non-polymer catechins in the packaged beverage of the present invention is 0.3 to 0.6, Furthermore, it is preferable from a viewpoint of bitterness suppression of non-polymer catechins that it is 0.4-0.6.

本発明における前記の高濃度の非重合体カテキン類を有する容器詰飲料は、例えば緑茶抽出物の濃縮物又は緑茶抽出物の精製物であるカテキン製剤を配合して非重合体カテキン類濃度を調整することにより得ることができる。ここでいう緑茶抽出物の濃縮物とは、生茶葉もしくは乾燥緑茶葉から熱水もしくは水溶性有機溶媒により抽出した溶液から水分を一部除去し、場合によっては精製して非重合体カテキン類濃度を高めたものであり、形態としては、固体、水溶液、スラリー状など種々のものが挙げられる。   The container-packed beverage having the high concentration non-polymer catechins in the present invention is prepared by, for example, blending a catechin preparation which is a concentrate of green tea extract or a purified product of green tea extract to adjust the concentration of non-polymer catechins Can be obtained. The concentrate of green tea extract as used herein refers to the concentration of non-polymer catechins by removing a portion of water from a solution extracted from fresh tea leaves or dried green tea leaves with hot water or a water-soluble organic solvent and purifying in some cases. Examples of the form include various forms such as solid, aqueous solution, and slurry.

非重合体カテキン類を含有する緑茶抽出物の濃縮物水溶液としては市販の三井農林(株)「ポリフェノン」、伊藤園(株)「テアフラン」、太陽化学(株)「サンフェノン」などを溶解したものが挙げられる。また、これらを精製したものを用いてもよい。精製の方法としては、例えば緑茶抽出物の濃縮物を水又は水とエタノールなどの有機溶媒の混合物に懸濁して生じた沈殿を除去し、次いで溶媒を留去する方法がある。あるいは茶葉から熱水もしくはエタノールなどの水溶性有機溶媒により抽出した抽出物を濃縮したものを更に精製したもの、あるいは抽出物を直接精製したものを用いてもよい。用いる緑茶抽出物の濃縮物水溶液の非重合体カテキン類含有量が0.8質量%未満の場合には、加熱殺菌後の最終容器詰飲料の非重合体カテキン類の含有量が十分ではなく、50質量%を超える濃度では高粘度となり溶解に長時間を要する。緑茶抽出物の濃縮物水溶液中の好ましい非重合体カテキン類濃度は0.9〜30質量%であり、より好ましくは1〜20質量%である。   Concentrated aqueous solution of green tea extract containing non-polymer catechins is a solution of commercially available Mitsui Norin Co., Ltd. “Polyphenone”, ITO EN Co., Ltd. “Theafuran”, Taiyo Kagaku Co., Ltd. “Sunphenon”, etc. Can be mentioned. Moreover, you may use what refine | purified these. As a purification method, for example, there is a method in which a precipitate formed by suspending a concentrate of a green tea extract in water or a mixture of water and an organic solvent such as ethanol is removed, and then the solvent is distilled off. Alternatively, a product obtained by further concentrating an extract extracted from tea leaves with hot water or a water-soluble organic solvent such as ethanol, or a product obtained by directly purifying the extract may be used. If the content of non-polymer catechins in the aqueous concentrate solution of green tea extract to be used is less than 0.8% by mass, the content of non-polymer catechins in the final packaged beverage after heat sterilization is not sufficient, If the concentration exceeds 50% by mass, the viscosity becomes high and dissolution takes a long time. The preferred non-polymer catechin concentration in the aqueous concentrate solution of green tea extract is 0.9 to 30% by mass, more preferably 1 to 20% by mass.

本発明に用いる緑茶抽出液の原料となる緑茶は、Camellia属、例えばC.sinensis、C.assamica及びやぶきた種、又はそれらの雑種から得られる茶葉から製茶された乾燥茶葉が挙げられる。当該製茶された乾燥茶葉には、煎茶、番茶、玉露、てん茶、釜炒り茶等の緑茶類がある。   The green tea used as the raw material for the green tea extract used in the present invention is a genus Camellia, such as C.I. sinensis, C.I. Examples include dried tea leaves produced from tea leaves obtained from assamica and Yabuki species, or hybrids thereof. The tea leaves produced include green teas such as sencha, bancha, gyokuro, tencha, and kettle roasted tea.

これらの緑茶類のうち、生産量、価格、風味の点から、一般的には煎茶の一番茶及び二番茶が使用される。一番茶は、カフェイン量、アミノ酸量、うま味強度比が高く、アミノ酸組成比が低い。二番茶は、一番茶と比較して、カフェイン量、アミノ酸量、うま味強度比が低く、アミノ酸組成比が高い。緑茶抽出物の濃縮物は、二番茶と比較して、カフェイン量、アミノ酸量とも少ない。   Of these green teas, Sencha and Ichibancha are generally used from the viewpoint of production, price, and flavor. Ichibancha has a high caffeine content, amino acid content, and umami intensity ratio, and a low amino acid composition ratio. Nibancha has a lower caffeine content, amino acid content, and umami intensity ratio, and a higher amino acid composition ratio, compared to Ichibancha. The green tea extract concentrate has less caffeine content and amino acid content than Nibancha.

一番茶のみを用いて製造するときは、抽出液の配合量が多くなると、カフェイン量が多く、生理活性上好ましくない。また、カフェイン由来の苦味が強くなる。二番茶のみを用いると、アミノ酸量が少なく、うま味を十分に得ることができない。また、緑茶抽出物の濃縮物のみでは緑茶感が少なく不適である。   When manufacturing using only the most tea, if the amount of the extract is increased, the amount of caffeine is large, which is not preferable in terms of physiological activity. Moreover, the bitterness derived from caffeine becomes strong. If only Nibancha is used, the amount of amino acids is small and umami cannot be obtained sufficiently. In addition, a green tea extract alone is not suitable because it has little green tea feeling.

このように、一番茶、二番茶、緑茶抽出物の濃縮物をそれぞれ単品で配合するだけでは、本発明の容器詰緑茶飲料を得ることはできない。しかし、これらを組み合わせることによって苦味及び渋味が抑制された容器詰緑茶飲料を得ることができる。また、一番茶、二番茶から抽出されたエキスや、テアニン、グルタミン酸又はその塩をそのまま配合しても、本発明の容器詰緑茶飲料を得ることができる。   Thus, the container-packed green tea beverage of the present invention cannot be obtained only by blending the concentrates of Ichibancha, Nibancha, and green tea extract individually. However, by combining these, a packaged green tea beverage in which bitterness and astringency are suppressed can be obtained. Moreover, the container-packed green tea drink of this invention can be obtained even if it mix | blends the extract extracted from Ichibancha, Nibancha, theanine, glutamic acid, or its salt as it is.

本発明で使用する緑茶抽出液を得る方法については、ニーダー抽出等の攪拌抽出又はドリップ抽出等従来の方法により行う。また抽出時に水に予めアスコルビン酸ナトリウム等の有機酸又は有機酸塩類を添加してもよい。緑茶組成液は、水を用いて通常の抽出条件で製造される。緑茶葉から抽出する時の水の温度は、風味の観点から50〜100(沸騰水)℃が好ましく、更に好ましくは60〜100(沸騰水)℃である。緑茶葉からの抽出時の水の量は、緑茶葉に対して5〜60重量倍が好ましく、更に好ましくは5〜40重量倍である。緑茶葉からの抽出時間は1〜60分が好ましく、より好ましくは1〜40分、更に好ましくは1〜30分である。   About the method of obtaining the green tea extract used by this invention, it carries out by conventional methods, such as stirring extraction, such as kneader extraction, or drip extraction. Moreover, you may add organic acids or organic acid salts, such as sodium ascorbate, to water at the time of extraction beforehand. The green tea composition liquid is produced under normal extraction conditions using water. The temperature of water when extracting from green tea leaves is preferably 50 to 100 (boiling water) ° C., more preferably 60 to 100 (boiling water) ° C. from the viewpoint of flavor. The amount of water at the time of extraction from green tea leaves is preferably 5 to 60 times by weight, more preferably 5 to 40 times by weight with respect to green tea leaves. The extraction time from green tea leaves is preferably 1 to 60 minutes, more preferably 1 to 40 minutes, and still more preferably 1 to 30 minutes.

本発明において、アミノ酸組成比は、(C)グルタミン酸又はその塩/(B)テアニン(質量比)で表す。   In the present invention, the amino acid composition ratio is represented by (C) glutamic acid or a salt thereof / (B) theanine (mass ratio).

うま味強度比は、カテキン量に対するうま味の強さである。グルタミン酸又はその塩はテアニンの30倍のうま味強度があるため(非特許文献1)、((B)+(C)×30)/(A)カテキン量(質量比)で表す。   The umami intensity ratio is the intensity of umami relative to the amount of catechin. Since glutamic acid or a salt thereof has umami strength 30 times that of theanine (Non-patent Document 1), it is represented by ((B) + (C) × 30) / (A) catechin amount (mass ratio).

本発明において苦味及び渋味低減効果を得るには、アミノ酸組成比は0.27以下であることが必要である。0.27よりも高いと、うま味の相乗効果による十分な苦味及び渋味低減効果が得られない。   In order to obtain a bitterness and astringency reduction effect in the present invention, the amino acid composition ratio needs to be 0.27 or less. If it is higher than 0.27, sufficient bitterness and astringency reduction effect due to the synergistic effect of umami cannot be obtained.

更に、うま味強度比は0.3以上であることが必要である。0.3よりも低いと、うま味強度が不足し、十分な苦味及び渋味の低減効果及び茶本来の風味が得られない。   Furthermore, the umami intensity ratio needs to be 0.3 or more. If it is lower than 0.3, the umami strength is insufficient, and sufficient bitterness and astringency reduction effects and the original flavor of tea cannot be obtained.

本発明の容器詰飲料中に含有する(E)カフェイン濃度は、好ましくは0.005〜0.04質量%、更に好ましくは0.01〜0.035質量%、更に好ましくは0.01〜0.03質量%、更に好ましくは0.01〜0.024質量%である。0.005質量%よりも少ないと、緑茶飲料としての嗜好性が低下し、0.04質量%よりも多いと、カフェイン自体の味の影響が大きくなるため好ましくない。   The concentration of (E) caffeine contained in the container-packed beverage of the present invention is preferably 0.005 to 0.04% by mass, more preferably 0.01 to 0.035% by mass, and still more preferably 0.01 to It is 0.03 mass%, More preferably, it is 0.01-0.024 mass%. If the amount is less than 0.005% by mass, the palatability as a green tea beverage is lowered. If the amount is more than 0.04% by mass, the influence of the taste of caffeine itself is undesirably increased.

本発明の容器詰飲料中に含有する(B)テアニン濃度は、好ましくは0.003〜0.03質量%、更に好ましくは0.005〜0.025質量%である。0.003質量%よりも少ないと、緑茶感を感じられず嗜好性が低下し、0.03質量%よりも多いと異味を感じる。本発明における抽出液又はカテキン製剤にテアニンが不足している場合には、市販品を配合してうま味強度比を向上させることができる。たとえば、テアニンとして、市販品のサンテアニン(太陽化学製)などを使用できる。   The (B) theanine concentration contained in the packaged beverage of the present invention is preferably 0.003 to 0.03% by mass, more preferably 0.005 to 0.025% by mass. If the amount is less than 0.003% by mass, the green tea feeling is not felt, and the palatability is lowered. When theanine is insufficient in the extract or catechin preparation of the present invention, a commercially available product can be blended to improve the umami strength ratio. For example, commercially available suntheanine (manufactured by Taiyo Kagaku) can be used as theanine.

本発明の容器詰飲料中の(C)グルタミン酸又はその塩の濃度は、好ましくは0.0008〜0.005質量%、更に好ましくは0.001〜0.004質量%である。0.0008質量%よりも少ないと、緑茶感を感じられず嗜好性が低下し、0.005質量%よりも多いと、塩味を感じ、嗜好性が低下する。   The concentration of (C) glutamic acid or a salt thereof in the packaged beverage of the present invention is preferably 0.0008 to 0.005 mass%, more preferably 0.001 to 0.004 mass%. If the amount is less than 0.0008% by mass, the green tea feeling is not felt and the palatability is lowered. If the amount is more than 0.005% by mass, the salty taste is felt and the palatability is lowered.

本発明の容器詰飲料中に含有するグルタミン酸又はその塩は、グルタミン酸、グルタミン酸Na、グルタミン酸K、グルタミン酸Ca、グルタミン酸Mg等のうち、一種又はそれらの混合物でもよい。これらのうち、溶解性の点においてグルタミン酸又はグルタミン酸Naが好ましい。本発明における抽出液又はカテキン製剤にグルタミン酸又はその塩を配合してうま味強度比を向上させることができる。たとえば、グルタミン酸ナトリウムとして市販品の味の素RCなどを使用できる。   The glutamic acid or salt thereof contained in the packaged beverage of the present invention may be one or a mixture of glutamic acid, glutamic acid Na, glutamic acid K, glutamic acid Ca, glutamic acid Mg, and the like. Of these, glutamic acid or Na glutamate is preferable in terms of solubility. Glutamic acid or a salt thereof can be added to the extract or catechin preparation of the present invention to improve the umami intensity ratio. For example, commercially available Ajinomoto RC can be used as sodium glutamate.

本発明における緑茶飲料は、苦味抑制剤を配合すると、飲用しやすくなり好ましい。苦味抑制剤は、水溶性高分子、サイクロデキストリンが好ましい。水溶性高分子としては、ペクチン、デキストリン等が挙げられる。サイクロデキストリンとしては、α−、β−、γ−サイクロデキストリン及び分岐α−、β−、γ−サイクロデキストリンが使用できる。また、クラスターデキストリンなど他のサイクロデキストリンを併用しても良い。β−サイクロデキストリンとしては例えば、セルデックスB−50K、セルデックスSL−20(日本食品化工社製)やデキシパールβ−100(塩水港精糖社製)などを使用できる。γ−サイクロデキストリンとしては例えば、CAVAMAX W8 Food(株式会社シクロケム:WACKER社製))等が挙げられる。   The green tea beverage in the present invention is preferably mixed with a bitterness inhibitor because it becomes easy to drink. The bitterness inhibitor is preferably a water-soluble polymer or cyclodextrin. Examples of the water-soluble polymer include pectin and dextrin. As the cyclodextrin, α-, β-, γ-cyclodextrin and branched α-, β-, γ-cyclodextrin can be used. Further, other cyclodextrins such as cluster dextrin may be used in combination. As β-cyclodextrin, for example, Celdex B-50K, Celdex SL-20 (manufactured by Nippon Shokuhin Kako Co., Ltd.), Dexipal β-100 (manufactured by Shisui Minato Sugar Co., Ltd.) and the like can be used. Examples of γ-cyclodextrin include CAVAMAX W8 Food (Cyclochem Co., Ltd .: manufactured by WACKER)).

本発明におけるサイクロデキストリンは、容器詰飲料中の濃度が0.01〜0.3質量%、特に0.01〜0.25質量%となるように添加するのが、最終容器詰飲料の苦味渋味低減化効果、風味低下抑制、更には高温保存後の色相改善効果の点で好ましい。   The cyclodextrin in the present invention is added so that the concentration in the packaged beverage is 0.01 to 0.3% by mass, and particularly 0.01 to 0.25% by mass. It is preferable in terms of a taste reducing effect, a flavor reduction suppression, and a hue improving effect after high temperature storage.

本発明の容器詰飲料には、茶由来の成分にあわせて、酸化防止剤、香料、各種エステル類、色素類、保存料、調味料、酸味料、果汁エキス類、野菜エキス類、花蜜エキス類、pH調整剤、品質安定剤などの添加剤を単独、あるいは併用して配合しても良い。   In the container-packed beverage of the present invention, an antioxidant, a fragrance, various esters, a pigment, a preservative, a seasoning, an acidulant, a fruit juice extract, a vegetable extract, a nectar extract, etc. , Additives such as pH adjusters and quality stabilizers may be used alone or in combination.

本発明における緑茶飲料のpHは、非重合体カテキン類の化学的安定性のため、25℃でpH4〜7であり、好ましくはpH4.5〜7、より好ましくはpH5〜7であることが好ましい。pHの調整は、酸化防止効果を持たせる効果も含めてアスコルビン酸を配合することが好ましい。また、炭酸水素ナトリウムを使用するときは、3〜30%水溶液で配合するのが好ましい。pHの調整が不要の場合は、アスコルビン酸の金属塩を配合することが好ましいが、具体的にはアスコルビン酸ナトリウムが好ましい。   The pH of the green tea beverage in the present invention is preferably pH 4-7 at 25 ° C., preferably pH 4.5-7, more preferably pH 5-7, due to the chemical stability of non-polymer catechins. . As for the adjustment of pH, it is preferable to blend ascorbic acid including the effect of giving an antioxidant effect. Moreover, when using sodium hydrogencarbonate, it is preferable to mix | blend with 3 to 30% aqueous solution. When pH adjustment is unnecessary, it is preferable to add a metal salt of ascorbic acid, but specifically sodium ascorbate is preferable.

本発明の容器詰飲料に使用される容器は、一般の飲料と同様にポリエチレンテレフタレートを主成分とする成形容器(いわゆるPETボトル)、金属缶、金属箔やプラスチックフィルムと複合された紙容器、瓶などの通常の形態で提供することができる。ここでいう容器詰飲料とは希釈せずに飲用できるものをいう。   The container used for the container-packed beverage of the present invention is a molded container (so-called PET bottle) mainly composed of polyethylene terephthalate, a metal can, a paper container combined with a metal foil or a plastic film, and a bottle as in the case of general beverages. Etc. can be provided in the usual form. The term “packaged beverage” as used herein means a beverage that can be drunk without dilution.

また本発明の容器詰飲料は、例えば、金属缶のような容器に充填後、加熱殺菌できる場合にあっては食品衛生法に定められた殺菌条件で製造される。PETボトル、紙容器のようにレトルト殺菌できないものについては、あらかじめ上記と同等の殺菌条件、例えばプレート式熱交換器などで高温短時間殺菌後、一定の温度迄冷却して容器に充填する等の方法が採用される。また無菌下で、充填された容器に別の成分を配合して充填してもよい。更に、酸性下で加熱殺菌後、無菌下でpHを中性に戻すことや、中性下で加熱殺菌後、無菌下でpHを酸性に戻すなどの操作も可能である。   Moreover, the container-packed drink of this invention is manufactured on the sterilization conditions prescribed | regulated to the food hygiene law, for example, when it can heat-sterilize after filling a container like a metal can. For PET bottles and paper containers that cannot be sterilized by retort, sterilize under the same conditions as above, for example, after sterilizing at high temperature and short time with a plate heat exchanger, etc. The method is adopted. Moreover, you may mix | blend another component with the filled container under aseptic conditions. Furthermore, after sterilizing under heat, the pH can be returned to neutral under aseptic conditions, or after sterilizing under heat under neutral conditions, the pH can be returned to acidic conditions under aseptic conditions.

非重合体カテキン類及びカフェインの測定
メンブランフィルター(0.8μm)でろ過し、次いで蒸留水で希釈した試料を、島津製作所製、高速液体クロマトグラフ(型式SCL−10AVP)を用い、オクタデシル基導入液体クロマトグラフ用パックドカラム L−カラムTM ODS(4.6mmφ×250mm:財団法人 化学物質評価研究機構製)を装着し、カラム温度35℃でグラジエント法により測定した。移動相A液は酢酸を0.1mol/L含有の蒸留水溶液、B液は酢酸を0.1mol/L含有のアセトニトリル溶液とし、試料注入量は20μL、UV検出器波長は280nmの条件で行った(通常カテキン類及びカフェインの濃度は、質量/体積%(%[w/v])で表すが、実施例中の含有量は液量を掛けて質量で示した)。
Measurement of non-polymer catechins and caffeine Octadecyl group was introduced using a high-performance liquid chromatograph (model SCL-10AVP) manufactured by Shimadzu Corporation, which was filtered through a membrane filter (0.8 μm) and then diluted with distilled water. Packed column for liquid chromatograph L-column TM ODS (4.6 mmφ × 250 mm: manufactured by Chemical Substances Research Institute) was mounted, and the measurement was performed at a column temperature of 35 ° C. by a gradient method. The mobile phase A solution was a distilled aqueous solution containing 0.1 mol / L of acetic acid, the B solution was an acetonitrile solution containing 0.1 mol / L of acetic acid, the sample injection amount was 20 μL, and the UV detector wavelength was 280 nm. (Normally, the concentrations of catechins and caffeine are expressed as mass / volume% (% [w / v]), but the contents in the examples are expressed by mass by multiplying the liquid amount).

グルタミン酸、テアニンの測定
メンブランフィルター(0.45μm)でろ過し、次いで蒸留水で希釈した試料を、島津製作所製、高速液体クロマトグラフ(型式SCL−10AVP)を用い、ポストカラム蛍光誘導体化法により測定した。Li型陽イオン交換カラム Shim−pack Amino−Li(100mmL.×6.0mmI.D.:島津製作所製)を装着し、カラム温度39℃でグラジエント法により測定した。移動相A液は0.15Nクエン酸リチウム(メチルセロソルブ含有)(pH2.6)、B液は0.3Nクエン酸リチウムと0.2Mホウ酸の混合溶液(pH10)、C液は0.2M水酸化リチウムとした。反応液としては、ホウ酸−炭酸Buffer(0.384M炭酸ナトリウム、0.216Mホウ酸、0.108M硫酸カリウムの混合溶液)1000mL中、o−フタルアルデヒド0.8g、エタノール0.4g、N−アセチルシステイン1g、ポリオキシエチレンラウリルエーテル0.4g含有している溶液を用いた。試料注入量は20μL、蛍光検出器は、RF−535、EX348nm、Em450mの条件で行った(通常アミノ酸類及びアミノ酸の濃度は、質量/体積%(%[w/v])で表すが、実施例中の含有量は液量を掛けて質量で示した)。
Measurement of glutamic acid and theanine A sample filtered through a membrane filter (0.45 μm) and then diluted with distilled water was measured by a post-column fluorescence derivatization method using a high-performance liquid chromatograph (model SCL-10AVP) manufactured by Shimadzu Corporation. did. A Li-type cation exchange column Shim-pack Amino-Li (100 mm L. × 6.0 mm ID: manufactured by Shimadzu Corporation) was mounted, and measurement was performed by a gradient method at a column temperature of 39 ° C. Mobile phase A solution is 0.15N lithium citrate (containing methyl cellosolve) (pH 2.6), B solution is a mixed solution of 0.3N lithium citrate and 0.2M boric acid (pH 10), and C solution is 0.2M. Lithium hydroxide was used. As a reaction solution, boric acid-carbonate buffer (0.384 M sodium carbonate, 0.216 M boric acid, 0.108 M potassium sulfate mixed solution) in 1000 mL, o-phthalaldehyde 0.8 g, ethanol 0.4 g, N- A solution containing 1 g of acetylcysteine and 0.4 g of polyoxyethylene lauryl ether was used. The sample injection amount was 20 μL, and the fluorescence detector was RF-535, EX348 nm, and Em450m (normal amino acids and amino acid concentrations are expressed in mass / volume% (% [w / v]). The content in the examples is expressed by mass multiplied by the liquid amount).

風味の評価
パネラー6名により飲用試験を行い、4段階評価した。
Evaluation of Flavor A drinking test was conducted by 6 panelists, and was evaluated in 4 stages.

実施例1
静岡産の二番茶の緑茶葉(1)135gを65℃に加熱したイオン交換水4kgに加えて5分間抽出し、次いで抽出液から茶葉を取り除き、熱交換器で25℃以下に冷却し、緑茶抽出液(1)(アミノ酸組成比(=グルタミン酸/テアニン質量比):0.39)を得た。また、静岡産の一番茶の緑茶葉(2)135gも緑茶葉(1)と同様に処理し、抽出液を得た。次にネル濾過により抽出液中の沈殿物や浮遊物を取り除き、円盤型デプスフィルター(ゼータプラス10C)で濾過し、緑茶抽出液(2)(アミノ酸組成比:0.19)を得た。一方、市販の緑茶抽出物の濃縮物(三井農林(株)「ポリフェノンHG」)100gを95質量%エタノール490.9gに分散させ、活性炭クラレコールGLC(クラレケミカル社製)20gと酸性白土ミズカエース#600(水澤化学社製)50gを投入後、約10分間攪拌を続けた。そして、95%エタノール水溶液409.1gを10分間かけて滴下したのち、室温のまま約30分間の攪拌処理を続けた。その後、2号濾紙で活性炭及び沈殿物を濾過し、更に、孔径0.2μmの濾紙で濾過を行った。最後に水350mLを加えて減圧濃縮することによって精製物(1)(非重合体カテキン類15.5質量%、アミノ酸組成比0.02、うま味強度比0.04)を得た。緑茶抽出液(1)205.4g、緑茶抽出液(2)68.7g、精製物(1)4.5g、β−サイクロデキストリンとγ−サイクロデキストリンの混合物(セルデックスSL−20、日本食品化工製)8.8g及びアスコルビン酸ナトリウム0.4gを加え希釈後、5%炭酸水素ナトリウム水溶液を用いてpH6.2に調整し全量を1.000gにした。このときアミノ酸組成比は0.22、うま味強度比(=(テアニン+グルタミン酸×30)/カテキン質量比)は0.39であった。これを、UHT殺菌しPETボトルに充填した。この飲料の組成、風味評価結果を表1に示す。
Example 1
Shizuoka green tea leaves (1) 135g was added to 4kg of ion-exchanged water heated to 65 ° C and extracted for 5 minutes. Then, the tea leaves were removed from the extract and cooled to 25 ° C or lower with a heat exchanger. Extract liquid (1) (amino acid composition ratio (= glutamic acid / theanine mass ratio): 0.39) was obtained. In addition, 135 g of green tea leaf (2) of the most tea from Shizuoka was treated in the same manner as green tea leaf (1) to obtain an extract. Next, precipitates and suspended matters in the extract were removed by flannel filtration, and filtered through a disk-type depth filter (Zeta Plus 10C) to obtain a green tea extract (2) (amino acid composition ratio: 0.19). On the other hand, 100 g of commercially available green tea extract concentrate (Mitsui Norin Co., Ltd. “Polyphenone HG”) was dispersed in 490.9 g of 95% by mass ethanol, 20 g of activated carbon Kuraray Coal GLC (manufactured by Kuraray Chemical Co., Ltd.) and acidic clay Mizuka Ace # After adding 50 g of 600 (manufactured by Mizusawa Chemical Co., Ltd.), stirring was continued for about 10 minutes. And after dripping 409.1g of 95% ethanol aqueous solution over 10 minutes, the stirring process for about 30 minutes was continued at room temperature. Thereafter, the activated carbon and the precipitate were filtered with No. 2 filter paper, and further filtered with a filter paper having a pore size of 0.2 μm. Finally, 350 mL of water was added and concentrated under reduced pressure to obtain a purified product (1) (15.5% by mass of non-polymer catechins, amino acid composition ratio 0.02, umami strength ratio 0.04). Green tea extract (1) 205.4 g, Green tea extract (2) 68.7 g, Purified product (1) 4.5 g, Mixture of β-cyclodextrin and γ-cyclodextrin (Seldex SL-20, Nippon Shokuhin Kako) 8.8 g) and 0.4 g of sodium ascorbate were added for dilution, and the pH was adjusted to 6.2 using a 5% aqueous sodium hydrogen carbonate solution to make the total amount 1.000 g. At this time, the amino acid composition ratio was 0.22, and the umami intensity ratio (= (theanine + glutamic acid × 30) / catechin mass ratio) was 0.39. This was UHT sterilized and filled into a PET bottle. Table 1 shows the composition and flavor evaluation results of this beverage.

実施例2
鹿児島産の一番茶の緑茶葉(3)400gを65℃に加熱したイオン交換水12kgに加えて5分間抽出し、次いで抽出液から茶葉を取り除き、熱交換器で25℃以下に冷却した。次にネル濾過により抽出液中の沈殿物や浮遊物を取り除き、円盤型デプスフィルター(ゼータプラス10C)及び孔径0.2μmの濾紙で濾過し、減圧濃縮して緑茶エキス(3)(アミノ酸組成比:0.18)を510g得た。
この緑茶エキス(3)0.34gと、実施例1の緑茶抽出液(1)20.54g、精製物(1)4.5gを用い、実施例1と同様の配合を行った。このときアミノ酸組成比は0.22、うま味強度比は0.38であった。これを、UHT殺菌しPETボトルに充填した。この飲料の組成、風味評価結果を表1に示す。
Example 2
400 g of green tea leaves (3) of Ichibancha from Kagoshima were added to 12 kg of ion-exchanged water heated to 65 ° C. and extracted for 5 minutes, and then the tea leaves were removed from the extract and cooled to 25 ° C. or lower with a heat exchanger. Next, precipitates and suspended matters in the extract are removed by flannel filtration, filtered through a disk-type depth filter (Zeta Plus 10C) and filter paper having a pore size of 0.2 μm, and concentrated under reduced pressure to obtain a green tea extract (3) (amino acid composition ratio). : 0.18) was obtained.
Using this green tea extract (3) 0.34 g, the green tea extract (1) 20.54 g of Example 1, and 4.5 g of purified product (1), the same formulation as in Example 1 was performed. At this time, the amino acid composition ratio was 0.22, and the umami intensity ratio was 0.38. This was UHT sterilized and filled into a PET bottle. Table 1 shows the composition and flavor evaluation results of this beverage.

実施例3
実施例1の緑茶抽出液(1)255.4gに、緑茶の低温連続抽出物を酵素処理したエキスである緑茶アロマエキス3を10g溶解した。これに、実施例1の精製物(1)4.5gを用い、実施例1と同様の配合を行った。このときアミノ酸組成比は0.26、うま味強度比は0.39であった。これを、UHT殺菌しPETボトルに充填した。この飲料の組成、風味評価結果を表1に示す。
Example 3
In 255.4 g of the green tea extract (1) of Example 1, 10 g of green tea aroma extract 3, which is an enzyme-treated extract of a low temperature continuous extract of green tea, was dissolved. To this, 4.5 g of the purified product (1) of Example 1 was used and the same formulation as in Example 1 was performed. At this time, the amino acid composition ratio was 0.26 and the umami intensity ratio was 0.39. This was UHT sterilized and filled into a PET bottle. Table 1 shows the composition and flavor evaluation results of this beverage.

実施例4
実施例1の緑茶抽出液(1)255.4gに、1%テアニン水溶液7.6g及び1%グルタミン酸Na0.3gを溶解した。これに、実施例1の精製物(1)4.5gを用い、実施例1と同様の配合を行った。このときアミノ酸組成比は0.12、うま味強度比は0.44であった。これを、UHT殺菌しPETボトルに充填した。この飲料の組成、風味評価結果を表1に示す。
Example 4
In 255.4 g of the green tea extract (1) of Example 1, 7.6 g of 1% theanine aqueous solution and 0.3 g of 1% sodium glutamate were dissolved. To this, 4.5 g of the purified product (1) of Example 1 was used and the same formulation as in Example 1 was performed. At this time, the amino acid composition ratio was 0.12, and the umami intensity ratio was 0.44. This was UHT sterilized and filled into a PET bottle. Table 1 shows the composition and flavor evaluation results of this beverage.

実施例5
実施例1の緑茶抽出液(2)557.3gに、実施例1の緑茶抽出液(1)と精製物(1)を添加しなかった以外は、実施例1と同様の配合を行った。このときアミノ酸組成比は0.19、うま味強度比は1.27であった。これを、UHT殺菌しPETボトルに充填した。この飲料の組成、風味評価結果を表1に示す。
Example 5
The same formulation as in Example 1 was performed, except that the green tea extract (1) and purified product (1) of Example 1 were not added to 557.3 g of the green tea extract (2) of Example 1. At this time, the amino acid composition ratio was 0.19 and the umami intensity ratio was 1.27. This was UHT sterilized and filled into a PET bottle. Table 1 shows the composition and flavor evaluation results of this beverage.

実施例6
市販の緑茶抽出物の濃縮物(三井農林(株)「ポリフェノンHG」)100gを95.0質量%エタノール490.9gに分散させ、活性炭クラレコールGLC(クラレケミカル社製)20gと酸性白土ミズカエース#600(水澤化学社製)50gを投入後、約10分間攪拌を続けた。そして、40%エタノール水溶液409.1gを10分間かけて滴下したのち、室温のまま約30分間の攪拌処理を続けた。その後、2号濾紙で活性炭及び沈殿物を濾過し、更に、孔径0.2μmの濾紙で濾過を行った。最後に水350mLを加えて減圧濃縮することによって精製物(2)(非重合体カテキン類14質量%、アミノ酸組成比0.16、うま味強度比0.36)を得た。
この精製物(2)8.7gと、緑茶抽出液(1)255.4gを用い、精製物(1)と緑茶抽出液(2)を添加しなかった以外は実施例1と同様の配合を行った。このときアミノ酸組成比は0.23、うま味強度比は0.45であった。これを、UHT殺菌しPETボトルに充填した。この飲料の組成、風味評価結果を表1に示す。
Example 6
100 g of a commercially available concentrate of green tea extract (Mitsui Norin Co., Ltd. “Polyphenone HG”) is dispersed in 490.9 g of 95.0% by mass ethanol, 20 g of activated carbon Kuraray Coal GLC (manufactured by Kuraray Chemical Co., Ltd.) and acidic clay Mizuka Ace # After adding 50 g of 600 (manufactured by Mizusawa Chemical Co., Ltd.), stirring was continued for about 10 minutes. Then, 409.1 g of 40% ethanol aqueous solution was dropped over 10 minutes, and then the stirring treatment was continued for about 30 minutes at room temperature. Thereafter, the activated carbon and the precipitate were filtered with No. 2 filter paper, and further filtered with a filter paper having a pore size of 0.2 μm. Finally, 350 mL of water was added and concentrated under reduced pressure to obtain a purified product (2) (non-polymer catechins 14 mass%, amino acid composition ratio 0.16, umami strength ratio 0.36).
The same formulation as in Example 1 was used except that 8.7 g of this purified product (2) and 255.4 g of green tea extract (1) were not added, and purified product (1) and green tea extract (2) were not added. went. At this time, the amino acid composition ratio was 0.23 and the umami intensity ratio was 0.45. This was UHT sterilized and filled into a PET bottle. Table 1 shows the composition and flavor evaluation results of this beverage.

比較例1
宮崎産の二番茶の緑茶葉(4)(アミノ酸組成比(=グルタミン酸/テアニン質量比):0.52)135gを実施例1の緑茶葉(1)と同様に処理し、抽出液を得た。緑茶抽出物(4)を205.4g添加し、緑茶葉(1)及び緑茶葉(2)を添加しなかった以外は、実施例1と同様の緑茶飲料を製造した。このときアミノ酸組成比は0.32、うま味強度比は0.32であった。この飲料の組成、風味評価結果を表2に示す。
Comparative Example 1
A green tea leaf (4) of the second tea from Miyazaki (4) (amino acid composition ratio (= glutamic acid / theanine mass ratio): 0.52) was treated in the same manner as the green tea leaf (1) of Example 1 to obtain an extract. . A green tea beverage similar to Example 1 was produced except that 205.4 g of the green tea extract (4) was added and the green tea leaf (1) and the green tea leaf (2) were not added. At this time, the amino acid composition ratio was 0.32, and the umami intensity ratio was 0.32. Table 2 shows the composition and flavor evaluation results of this beverage.

比較例2
実施例4の緑茶抽出液(1)255.4gに、実施例4の1%テアニン水溶液7.6gを添加しなかった以外は、実施例4と同様の配合を行った。このときアミノ酸組成比は0.34、うま味強度比は0.38であった。これを、UHT殺菌しPETボトルに充填した。この飲料の組成、風味評価結果を表2に示す。
Comparative Example 2
The same formulation as in Example 4 was performed, except that 7.6 g of the 1% aqueous theanine solution of Example 4 was not added to 255.4 g of the green tea extract (1) of Example 4. At this time, the amino acid composition ratio was 0.34 and the umami intensity ratio was 0.38. This was UHT sterilized and filled into a PET bottle. Table 2 shows the composition and flavor evaluation results of this beverage.

比較例3
実施例5の緑茶抽出液(2)12.4gに、実施例1の精製物(1)7gを用い、実施例5と同様の配合を行った。このときアミノ酸組成比は0.14、うま味強度比は0.28であった。これを、UHT殺菌しPETボトルに充填した。この飲料の組成、風味評価結果を表2に示す。
Comparative Example 3
7 g of the purified product (1) of Example 1 was used for 12.4 g of the green tea extract (2) of Example 5, and the same blending as in Example 5 was performed. At this time, the amino acid composition ratio was 0.14 and the umami intensity ratio was 0.28. This was UHT sterilized and filled into a PET bottle. Table 2 shows the composition and flavor evaluation results of this beverage.

Figure 0004768534
Figure 0004768534

Figure 0004768534
Figure 0004768534

表1及び2から明らかなように、(A)高濃度の非重合体カテキン類を含有し、且つ(B)テアニン0.003〜0.03質量%、(C)グルタミン酸0.0008〜0.005質量%を含有し、(C)/(B)が0.27質量比以下、((B)+(C)×30)/(A)が0.3質量比以上である容器詰緑茶飲料は、相乗的に苦味及び渋味が抑制される。   As is apparent from Tables 1 and 2, (A) contains a high concentration of non-polymer catechins, and (B) 0.003 to 0.03% by mass of theanine, (C) glutamic acid 0.0008 to 0.00. 005% by mass, (C) / (B) is 0.27 mass ratio or less, and ((B) + (C) × 30) / (A) is 0.3 mass ratio or more. Is synergistically suppressed in bitterness and astringency.

Claims (6)

次の成分(A)〜(C):
(A)非重合体カテキン類 0.1〜0.5質量%、
(B)テアニン 0.003〜0.03質量%、
(C)グルタミン酸又はその塩 0.0008〜0.004質量%、
を含有し、
(C)/(B)が0.27以下で、かつ((B)+(C)×30)/(A)が0.3〜0.45である容器詰緑茶飲料。
The following components (A) to (C):
(A) Non-polymer catechins 0.1 to 0.5% by mass,
(B) Theanine 0.003-0.03% by mass,
(C) glutamic acid or a salt thereof 0.0008 to 0.004 % by mass,
Containing
A packaged green tea beverage having (C) / (B) of 0.27 or less and ((B) + (C) × 30) / (A) of 0.3 to 0.45 .
緑茶抽出物の精製物を配合したものである請求項1記載の容器詰緑茶飲料。   The packaged green tea beverage according to claim 1, which is a blend of a purified product of green tea extract. (A)非重合体カテキン類中の(D)非重合体カテキンガレート体類の割合(D)/(A)が0.3〜0.6である請求項1又は2記載の容器詰緑茶飲料。   (A) The ratio (D) / (A) of (D) non-polymer catechin gallates in non-polymer catechins is 0.3 to 0.6. . (E)カフェインを0.005〜0.04質量%含有する請求項1〜3のいずれか一項記載の容器詰緑茶飲料。   (E) The container-packed green tea drink according to any one of claims 1 to 3 containing 0.005 to 0.04 mass% of caffeine. (C)グルタミン酸又はその塩が、グルタミン酸、グルタミン酸Na、グルタミン酸K、グルタミン酸Ca及びグルタミン酸Mgから選ばれる1種又は2種以上である請求項1〜4のいずれか一項記載の容器詰緑茶飲料。   (C) Glutamic acid or its salt is 1 type (s) or 2 or more types chosen from glutamic acid, glutamic acid Na, glutamic acid K, glutamic acid Ca, and glutamic acid Mg, The container-packed green tea drink as described in any one of Claims 1-4. (F)サイクロデキストリンを0.01〜0.3質量%含有する請求項1〜5のいずれか一項記載の容器詰緑茶飲料。   (F) The container-packed green tea drink according to any one of claims 1 to 5, which contains 0.01 to 0.3% by mass of cyclodextrin.
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