JP4713688B1 - Bioethanol production method - Google Patents
Bioethanol production method Download PDFInfo
- Publication number
- JP4713688B1 JP4713688B1 JP2010252747A JP2010252747A JP4713688B1 JP 4713688 B1 JP4713688 B1 JP 4713688B1 JP 2010252747 A JP2010252747 A JP 2010252747A JP 2010252747 A JP2010252747 A JP 2010252747A JP 4713688 B1 JP4713688 B1 JP 4713688B1
- Authority
- JP
- Japan
- Prior art keywords
- aqueous solution
- citric acid
- caustic
- plant
- plant body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 10
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 81
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 36
- 239000007864 aqueous solution Substances 0.000 claims abstract description 36
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 26
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims abstract description 21
- 239000000243 solution Substances 0.000 claims abstract description 19
- 229920002678 cellulose Polymers 0.000 claims abstract description 13
- 239000001913 cellulose Substances 0.000 claims abstract description 13
- 235000011121 sodium hydroxide Nutrition 0.000 claims abstract description 12
- 239000003518 caustics Substances 0.000 claims abstract description 10
- 235000011118 potassium hydroxide Nutrition 0.000 claims abstract description 7
- 241000196324 Embryophyta Species 0.000 claims description 38
- 238000000855 fermentation Methods 0.000 claims description 17
- 230000004151 fermentation Effects 0.000 claims description 17
- 238000010438 heat treatment Methods 0.000 claims description 16
- 239000003513 alkali Substances 0.000 claims description 10
- 241000220259 Raphanus Species 0.000 claims description 5
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 13
- 239000002028 Biomass Substances 0.000 abstract description 5
- 239000000284 extract Substances 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 11
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 9
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 238000010979 pH adjustment Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000255925 Diptera Species 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 241000218645 Cedrus Species 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000218691 Cupressaceae Species 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 235000020289 caffè mocha Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
【課題】植物体であるバイオマスを簡単な方法で効率よくエタノール化する方法を提供する。
【解決手段】セルロースを含有する植物体をｐＨ１２以上のカセイカリまたはカセイソーダの水溶液に浸漬するアルカリ処理工程、前記アルカリ処理工程で処理された植物体をｐＨ１〜５のクエン酸水溶液に浸漬し該クエン酸水溶液とともに９０〜１４０℃に加熱し、植物体を浸漬した状態の前記クエン酸水溶液にカセイカリまたはカセイソーダを加えて該クエン酸水溶液のｐＨを５を越えて７．５以下になるように調整し、調整された水溶液を発酵させる、発酵した水溶液からエタノールを抽出するバイオエタノールの製造方法である。
【選択図】なしThe present invention provides a method for efficiently ethanolizing biomass which is a plant body by a simple method.
An alkaline treatment step of immersing a plant body containing cellulose in an aqueous solution of caustic or caustic soda having a pH of 12 or more, and immersing the plant body treated in the alkaline treatment step in an aqueous citric acid solution of pH 1 to 5 Heat to 90-140 ° C. with an aqueous solution, add caustic potash or caustic soda to the citric acid aqueous solution in a state where the plant body is immersed, and adjust the pH of the citric acid aqueous solution to more than 5 to 7.5 or less, It is the manufacturing method of the bioethanol which extracts the ethanol from the fermented aqueous solution which ferments the adjusted aqueous solution.
[Selection figure] None
Description
本発明は、バイオエタノールを得る製造方法に関する。 The present invention relates to a production method for obtaining bioethanol.
バイオエタノールの製造方法としては、例えば、バイオマスの水スラリーを圧力４〜４０ＭＰａに加圧し、加圧された水スラリーを火力発電装置のボイラから供給される蒸気と反応器内で熱交換させることにより前記圧力での飽和温度以下かつ２５０〜４００℃の範囲の温度に加熱して前記バイオマスに含有されるヘミセルロースおよびセルロースを加水分解して糖を生成し、生成された糖を発酵させるバイオエタノールの製造方法が知られている。（例えば、特許文献１参照）また、セルロースを酵素糖化させて得た糖を発酵させてエタノールを得る方法が知られている。（例えば、特許文献２参照） As a method for producing bioethanol, for example, a biomass water slurry is pressurized to a pressure of 4 to 40 MPa, and the pressurized water slurry is subjected to heat exchange in a reactor with steam supplied from a boiler of a thermal power generator. Production of bioethanol that is heated to a temperature equal to or lower than the saturation temperature at the pressure and in the range of 250 to 400 ° C. to hydrolyze hemicellulose and cellulose contained in the biomass to produce sugar, and to ferment the produced sugar The method is known. (For example, refer patent document 1) Moreover, the method of fermenting the saccharide | sugar obtained by carrying out the enzymatic saccharification of a cellulose and obtaining ethanol is known. (For example, see Patent Document 2)
これらは大規模な高圧装置あるいは高価なセルロース糖化酵素を使用することが工業化のうえでネックとなっている。 The use of a large-scale high-pressure apparatus or an expensive cellulose saccharifying enzyme has become a bottleneck in industrialization.
本発明の目的は、植物体であるバイオマスを簡単な方法で効率よくエタノール化する方法を提供しようとすることである。 An object of the present invention is to provide a method for efficiently ethanolizing biomass that is a plant body by a simple method.
本発明の要旨とするところは、セルロースを含有する植物体をｐＨ１２以上のカセイカリまたはカセイソーダの水溶液に浸漬するアルカリ処理工程、
前記アルカリ処理工程で処理された植物体をｐＨ１〜５のクエン酸水溶液に浸漬し該クエン酸水溶液とともに９０〜１４０℃に加熱する加熱工程、
前記植物体を浸漬した状態の前記クエン酸水溶液にカセイカリまたはカセイソーダを加えて該クエン酸水溶液のｐＨを５を越えて７．５以下になるように調整するｐＨ調整工程、
調整された水溶液を発酵させる発酵工程、
発酵した水溶液からエタノールを抽出する抽出工程
を含むバイオエタノールの製造方法であることにある。
The gist of the present invention is that an alkali treatment step of immersing a plant containing cellulose in an aqueous solution of caustic potash or caustic soda having a pH of 12 or more,
A heating step of immersing the plant treated in the alkali treatment step in a citric acid aqueous solution having a pH of 1 to 5 and heating the plant to 90 to 140 ° C. with the citric acid aqueous solution;
PH adjusting step of adjusting caustic acid or caustic soda to the citric acid aqueous solution in a state in which the plant body is immersed to adjust the pH of the citric acid aqueous solution to more than 5 and not more than 7.5,
A fermentation process for fermenting the adjusted aqueous solution,
It exists in the manufacturing method of bioethanol including the extraction process which extracts ethanol from fermented aqueous solution.
前記バイオエタノールの製造方法においては、前記発酵工程が、前記ｐＨ調整工程で調整された水溶液にさらに大根を加えた液を発酵させる工程であり得る。 In the bioethanol production method, the fermentation step may be a step of fermenting a solution obtained by adding radish to the aqueous solution adjusted in the pH adjustment step.
本発明によると、植物体であるバイオマスから簡単な方法で効率よくバイオエタノールを得る製造方法が提供される。 ADVANTAGE OF THE INVENTION According to this invention, the manufacturing method which obtains bioethanol efficiently by the simple method from the biomass which is a plant body is provided.
本発明のバイオエタノールの製造方法は、
セルロースを含有する植物体をｐＨ１２以上のカセイカリまたはカセイソーダの水溶液に浸漬するアルカリ処理工程、
前記アルカリ処理工程で処理された植物体をｐＨ１〜５のクエン酸水溶液に浸漬し該クエン酸水溶液とともに９０〜１４０℃に加熱する加熱工程、
前記植物体を浸漬した状態の前記クエン酸水溶液にカセイカリまたはカセイソーダを加えて該クエン酸水溶液のｐＨを５を越えて７．５以下になるように調整するｐＨ調整工程、
調整された水溶液を発酵させる発酵工程、
発酵した水溶液からエタノールを抽出する抽出工程
を含んで構成される。
The method for producing bioethanol of the present invention comprises:
An alkali treatment step of immersing a plant containing cellulose in an aqueous solution of caustic potash or caustic soda having a pH of 12 or more;
A heating step of immersing the plant treated in the alkali treatment step in a citric acid aqueous solution having a pH of 1 to 5 and heating the plant to 90 to 140 ° C. with the citric acid aqueous solution;
PH adjusting step of adjusting caustic acid or caustic soda to the citric acid aqueous solution in a state in which the plant body is immersed to adjust the pH of the citric acid aqueous solution to more than 5 and not more than 7.5,
A fermentation process for fermenting the adjusted aqueous solution,
It includes an extraction process for extracting ethanol from the fermented aqueous solution.
本発明に用いられる植物体はセルロースを含有するものであり、ほかにリグノセルロースが含有されていてもよい。本発明に用いられる植物体としては、松、杉、檜、その他の樹木などの山野に生息する樹木や山野に生息する草木類や湖沼などの水系環境に生息する藻類、水草類が例示される。 The plant used in the present invention contains cellulose and may contain lignocellulose in addition. Examples of plants used in the present invention include trees that inhabit mountains such as pine, cedar, cypress, and other trees, plants and trees that inhabit the mountains, and algae and aquatic plants that inhabit aquatic environments such as lakes. .
上記アルカリ処理工程にあっては、カセイカリまたはカセイソーダの水溶液１００重量部に対して植物体が５〜１５重量部（乾燥重量）用いられることが好ましい。アルカリ処理工程における処理液の温度は室温〜１００℃が好ましい。処理時間は、処理液の温度が室温の場合は２０〜４０時間が好ましい。適した処理時間は処理液の温度が高いほど短くなり、処理液の温度が１００℃の場合は１〜２分が好ましい。処理時間が長くなりすぎると液が黒ずんできて、上記加熱工程におけるセルロースの糖化が阻害される。 In the alkali treatment step, it is preferable to use 5 to 15 parts by weight (dry weight) of the plant body with respect to 100 parts by weight of the caustic potash or caustic soda aqueous solution. The temperature of the treatment liquid in the alkali treatment step is preferably room temperature to 100 ° C. The treatment time is preferably 20 to 40 hours when the temperature of the treatment liquid is room temperature. A suitable treatment time is shorter as the temperature of the treatment liquid is higher. When the temperature of the treatment liquid is 100 ° C., 1 to 2 minutes is preferable. If the treatment time is too long, the liquid will darken and saccharification of cellulose in the heating step will be inhibited.
カセイカリまたはカセイソーダの水溶液には植物体の処理によりリグニン等の非セルロースの多糖類を含む不純物が溶出するので、アルカリ処理によりこれら不純物が植物体から除去され後工程での発酵が効率よく行われる。上記加熱工程にあっては、アルカリ処理工程で処理された植物体を水洗したのちｐＨ１〜５のクエン酸水溶液に浸漬し該クエン酸水溶液とともに９０〜１４０℃に加熱することが好ましい。加熱時間は１０〜５０時間が好ましい。上記加熱工程により、植物体のセルロースが糖化される。この加熱工程においてはクエン酸水溶液１００重量部に対して植物体がもとの乾燥重量換算で５〜１５重量部投入されていることが好ましい。 Impurities containing non-cellulose polysaccharides such as lignin are eluted in the aqueous solution of caustic potash or caustic soda by the treatment of the plant body, so that these impurities are removed from the plant body by the alkali treatment, and fermentation in the subsequent process is efficiently performed. In the heating step, it is preferable that the plant treated in the alkali treatment step is washed with water and then immersed in an aqueous citric acid solution having a pH of 1 to 5 and heated to 90 to 140 ° C. together with the aqueous citric acid solution. The heating time is preferably 10 to 50 hours. The cellulose of a plant body is saccharified by the said heating process. In this heating step, it is preferable that 5 to 15 parts by weight of the plant body in terms of the original dry weight is added to 100 parts by weight of the citric acid aqueous solution.
上記ｐＨ調整工程においては、処理された植物体を含む加熱工程後の処理液を室温あるいは室温に近い温度に冷却した後カセイカリまたはカセイソーダを加えることが好ましい。また、この冷却の前または後に処理された植物体を含む加熱工程後の処理液をミキサーで攪拌することが好ましい。 In the pH adjustment step, it is preferable to add caustic potash or caustic soda after cooling the treatment liquid after the heating step containing the treated plant body to room temperature or a temperature close to room temperature. Moreover, it is preferable to stir the process liquid after the heating process containing the plant body processed before or after this cooling with a mixer.
上記発酵工程にあっては、ｐＨ調整工程で処理された植物体を含む加熱工程後の処理液に酵素、酵母、または微生物を添加して発酵を行わせる。ｐＨ調整工程で処理された植物体を含む加熱工程後の処理液を５時間以上、特に１０〜１００時間発酵可能温度に保つことが好ましい。この発酵可能温度としては酵素や酵母の種類にもよるが２０〜３７℃が好ましい。この発酵過程において、発酵にかかわる酵素がクエン酸塩に作用し、この作用が発酵の促進に寄与する。 In the said fermentation process, an enzyme, yeast, or microorganisms are added to the processing liquid after the heating process containing the plant body processed at the pH adjustment process, and fermentation is performed. It is preferable to keep the treatment solution after the heating step including the plant body treated in the pH adjustment step at a temperature allowing fermentation for 5 hours or more, particularly 10 to 100 hours. The fermentable temperature is preferably 20 to 37 ° C, although it depends on the type of enzyme or yeast. In this fermentation process, enzymes involved in fermentation act on citrate, and this action contributes to the promotion of fermentation.
また、上記発酵工程にあっては、ｐＨ調整工程で処理された植物体を含む加熱工程後の処理液に酵素、酵母、または微生物と大根を添加して発酵を行わせることがさらに好ましい。大根は当初投入された植物体１００重量部に対して３〜５重量部添加されることが好ましい。これにより、エタノールの収率が２〜３割程度向上する。 Moreover, in the said fermentation process, it is still more preferable to add an enzyme, yeast, or microorganisms, and a radish to the processing liquid after the heating process containing the plant body processed at the pH adjustment process, and to perform fermentation. The radish is preferably added in an amount of 3 to 5 parts by weight with respect to 100 parts by weight of the plant body initially charged. Thereby, the yield of ethanol improves about 20-30%.
上記発酵工程において、処理液中の糖がエタノールに転化する。 In the fermentation process, the sugar in the treatment liquid is converted to ethanol.
上記抽出工程における、液中のエタノールを抽出する方法は特に限定されず、例えば、蒸留により多段濃縮する常法を用いることができる。 The method for extracting ethanol in the liquid in the extraction step is not particularly limited, and for example, a conventional method of multi-stage concentration by distillation can be used.
本発明により、乾燥した植物体に対して１５重量％以上のエタノールを得ることができる。 According to the present invention, 15% by weight or more of ethanol can be obtained based on the dried plant body.
本発明の植物体糖化方法にあっては、草木類を用いる場合、カセイカリまたはカセイソーダの水溶液に浸漬する前に草木類を加圧盤で挟んで加圧して形態をほぐすことが糖化の効率上好ましい。 In the plant saccharification method of the present invention, when using vegetation, it is preferable from the viewpoint of the efficiency of saccharification that the plant is squeezed with a pressure plate before being dipped in an aqueous solution of caustic or caustic soda to press the plant.
本発明により植物体に含まれるセルロースを効率よく糖化することができる。また、本発明は高圧装置や高価なセルロース糖化酵素を購入して使用することがないので経済的である。 According to the present invention, cellulose contained in a plant can be efficiently saccharified. Further, the present invention is economical because it does not purchase and use a high-pressure apparatus or an expensive cellulose saccharifying enzyme.
実施例１
試料として琵琶湖に生息している水草（オオカナダモ）（乾燥重量３００ｇ）を用い、ｐＨ１３のカセイカリ水溶液３Ｌに２４時間浸漬したのち水洗し、ｐＨ２のクエン酸水溶液４Ｌに投入した。この試料を投入状態のクエン酸水溶液を１２１℃に昇温し、１０時間この温度にキープした。次いでこの試料を投入状態のクエン酸水溶液を室温に自然冷却後カセイカリを加えて中和し、ミキサーで攪拌した。次いでこの液に酵母（白神コダマ酵母 ドライ）を２ｇ加えて室温で２４時間発酵させた。発酵液を２段蒸留して濃度８０重量％のエタノール水溶液５０ｍＬを得た。
Example 1
As a sample, aquatic plants (Ocana mosquito) inhabiting Lake Biwa (dry weight 300 g) were immersed in 3 L of caustic solution of pH 13 for 24 hours, washed with water, and put into 4 L of citric acid solution of pH 2. The aqueous citric acid solution in which this sample was charged was heated to 121 ° C. and kept at this temperature for 10 hours. Then, the citric acid aqueous solution in which this sample was charged was naturally cooled to room temperature, neutralized by adding caustic, and stirred with a mixer. Next, 2 g of yeast (Shirakami Kodama Yeast Dry) was added to this solution and fermented at room temperature for 24 hours. The fermentation broth was distilled in two stages to obtain 50 mL of an aqueous ethanol solution having a concentration of 80% by weight.
実施例２
試料として琵琶湖に生息している水草（オオカナダモ）（乾燥重量３００ｇ）を用い、ｐＨ１３のカセイカリ水溶液３Ｌに２４時間浸漬したのち水洗し、ｐＨ２のクエン酸水溶液４Ｌに投入した。この試料を投入状態のクエン酸水溶液を１２１℃に昇温し、１０時間この温度にキープした。次いでこの試料を投入状態のクエン酸水溶液を室温に自然冷却後カセイカリを加えて中和し、次いでこの液に大根１５ｇを加えてミキサーで攪拌した。次にこの液に酵母（白神コダマ酵母 ドライ）を２ｇと室温で２４時間発酵させた。発酵液を２段蒸留して濃度８０重量％のエタノール水溶液６７ｍＬを得た。
Example 2
As a sample, aquatic plants (Ocana mosquito) inhabiting Lake Biwa (dry weight 300 g) were immersed in 3 L of caustic solution of pH 13 for 24 hours, washed with water, and put into 4 L of citric acid solution of pH 2. The aqueous citric acid solution in which this sample was charged was heated to 121 ° C. and kept at this temperature for 10 hours. Next, the citric acid aqueous solution in which the sample was charged was naturally cooled to room temperature, neutralized by adding caustic, and then 15 g of radish was added to this solution and stirred with a mixer. Next, 2 g of yeast (Shirakami Kodama yeast dry) was fermented to this solution at room temperature for 24 hours. The fermentation broth was distilled in two stages to obtain 67 mL of an aqueous ethanol solution having a concentration of 80% by weight.
比較例１
試料として琵琶湖に生息している水草（オオカナダモ）（乾燥重量３００ｇ）を用い、ｐＨ２のクエン酸水溶液４Ｌに投入した。この試料を投入状態のクエン酸水溶液を１２１℃に昇温し、１０時間この温度にキープした。次いでこの試料を投入状態のクエン酸水溶液を室温に自然冷却後カセイカリを加えて中和し、ミキサーで攪拌した。次いでこの液に酵母（白神コダマ酵母 ドライ）を２ｇ加えて室温で２４時間発酵させた。発酵液を２段蒸留したがエタノールの生成は認められなかった。
Comparative Example 1
As a sample, aquatic plants (Ocana mocha) inhabiting Lake Biwa (dry weight 300 g) were used and introduced into 4 L of citric acid aqueous solution at pH 2. The aqueous citric acid solution in which this sample was charged was heated to 121 ° C. and kept at this temperature for 10 hours. Then, the citric acid aqueous solution in which this sample was charged was naturally cooled to room temperature, neutralized by adding caustic, and stirred with a mixer. Next, 2 g of yeast (Shirakami Kodama Yeast Dry) was added to this solution and fermented at room temperature for 24 hours. The fermentation broth was distilled in two stages, but no ethanol was observed.
本発明により得られるエタノールは石油系の燃料の代替として好適に使用できる。 Ethanol obtained by the present invention can be suitably used as an alternative to petroleum-based fuels.
Claims (1)
前記アルカリ処理工程で処理された植物体をｐＨ１〜５のクエン酸水溶液に浸漬し該クエン酸水溶液とともに９０〜１４０℃に加熱する加熱工程、
前記植物体を浸漬した状態の前記クエン酸水溶液にカセイカリまたはカセイソーダを加えて該クエン酸水溶液のｐＨを５を越えて７．５以下になるように調整するｐＨ調整工程、
調整された水溶液にさらに大根を加えた液を発酵させる発酵工程、
発酵した水溶液からエタノールを抽出する抽出工程
を含むバイオエタノールの製造方法。 An alkali treatment step of immersing a plant containing cellulose in an aqueous solution of caustic potash or caustic soda having a pH of 12 or more;
A heating step of immersing the plant treated in the alkali treatment step in a citric acid aqueous solution having a pH of 1 to 5 and heating the plant to 90 to 140 ° C. with the citric acid aqueous solution;
PH adjusting step of adjusting caustic acid or caustic soda to the citric acid aqueous solution in a state in which the plant body is immersed to adjust the pH of the citric acid aqueous solution to more than 5 and not more than 7.5,
A fermentation process for fermenting a solution obtained by adding radish to the prepared aqueous solution;
A method for producing bioethanol comprising an extraction step of extracting ethanol from a fermented aqueous solution .
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2010252747A JP4713688B1 (en) | 2010-11-11 | 2010-11-11 | Bioethanol production method |
| BRPI1104975-8A BRPI1104975A2 (en) | 2010-11-11 | 2011-09-22 | bioethanol production method |
| PCT/JP2011/076338 WO2012063958A1 (en) | 2010-11-11 | 2011-11-09 | Process for production of bioethanol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2010252747A JP4713688B1 (en) | 2010-11-11 | 2010-11-11 | Bioethanol production method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP4713688B1 true JP4713688B1 (en) | 2011-06-29 |
| JP2012100594A JP2012100594A (en) | 2012-05-31 |
Family
ID=44292670
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010252747A Expired - Fee Related JP4713688B1 (en) | 2010-11-11 | 2010-11-11 | Bioethanol production method |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JP4713688B1 (en) |
| BR (1) | BRPI1104975A2 (en) |
| WO (1) | WO2012063958A1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014132052A (en) * | 2013-01-07 | 2014-07-17 | Oji Holdings Corp | Fuel composition |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6328597B2 (en) * | 1982-11-16 | 1988-06-09 | Shinnenryoyu Kaihatsu Gijutsu Kenkyu Kumiai | |
| JPH04179488A (en) * | 1990-11-09 | 1992-06-26 | Agency Of Ind Science & Technol | Continuous method for fermenting alcohol using agglutinative microorganism |
| JP2006281024A (en) * | 2005-03-31 | 2006-10-19 | Tsukishima Kikai Co Ltd | Adsorbent, its manufacturing method and method for producing alcohol or organic acid |
| WO2008090707A1 (en) * | 2007-01-25 | 2008-07-31 | Japan International Research Center For Agricultural Sciences | Method for production of ethanol or lactic acid |
| JP2010088421A (en) * | 2008-09-11 | 2010-04-22 | Yasuo Fukutani | Production method of bioethanol |
| WO2010050223A1 (en) * | 2008-10-30 | 2010-05-06 | 王子製紙株式会社 | Saccharide production process and ethanol production process |
| JP2010518850A (en) * | 2007-02-26 | 2010-06-03 | コリア インスティチュート オブ インダストリアル テクノロジー | Production method of biofuel using seaweed |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4770987B2 (en) * | 2008-12-09 | 2011-09-14 | 東レ株式会社 | Method for producing sugar solution |
-
2010
- 2010-11-11 JP JP2010252747A patent/JP4713688B1/en not_active Expired - Fee Related
-
2011
- 2011-09-22 BR BRPI1104975-8A patent/BRPI1104975A2/en not_active IP Right Cessation
- 2011-11-09 WO PCT/JP2011/076338 patent/WO2012063958A1/en active Application Filing
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6328597B2 (en) * | 1982-11-16 | 1988-06-09 | Shinnenryoyu Kaihatsu Gijutsu Kenkyu Kumiai | |
| JPH04179488A (en) * | 1990-11-09 | 1992-06-26 | Agency Of Ind Science & Technol | Continuous method for fermenting alcohol using agglutinative microorganism |
| JP2006281024A (en) * | 2005-03-31 | 2006-10-19 | Tsukishima Kikai Co Ltd | Adsorbent, its manufacturing method and method for producing alcohol or organic acid |
| WO2008090707A1 (en) * | 2007-01-25 | 2008-07-31 | Japan International Research Center For Agricultural Sciences | Method for production of ethanol or lactic acid |
| JP2010518850A (en) * | 2007-02-26 | 2010-06-03 | コリア インスティチュート オブ インダストリアル テクノロジー | Production method of biofuel using seaweed |
| JP2010088421A (en) * | 2008-09-11 | 2010-04-22 | Yasuo Fukutani | Production method of bioethanol |
| WO2010050223A1 (en) * | 2008-10-30 | 2010-05-06 | 王子製紙株式会社 | Saccharide production process and ethanol production process |
Also Published As
| Publication number | Publication date |
|---|---|
| BRPI1104975A2 (en) | 2013-03-26 |
| JP2012100594A (en) | 2012-05-31 |
| WO2012063958A1 (en) | 2012-05-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5633839B2 (en) | Method for converting lignocellulosic biomass | |
| CN104774876B (en) | A kind of method of lignocellulose biomass comprehensive utilization | |
| WO2015005589A1 (en) | Method for preparing sugar, bioethanol or microbial metabolite from lignocellulosic biomass | |
| Singh et al. | Assessment of different pretreatment technologies for efficient bioconversion of lignocellulose to ethanol | |
| CN110272509A (en) | A kind of fiber biomass high-efficiency pretreatment separation hemicellulose and its method of comprehensive utilization | |
| JP2006075007A (en) | Method for pretreating lignocellulose and method for producing ethanol | |
| JP2005027541A (en) | Method for producing monosaccharides and / or oligosaccharides and method for separating wood component | |
| CN101538597A (en) | Method for disposing straws by sulfur trioxide gas | |
| JP2007151433A (en) | Method for pretreatment of lignocellulose and method for producing ethanol | |
| JP2012055302A (en) | Method for producing ethanol | |
| JP5504397B2 (en) | Ethanol production method | |
| JP5700619B2 (en) | Method for producing alcohol | |
| JP4713688B1 (en) | Bioethanol production method | |
| CN104762333B (en) | The method that xylitol is prepared using winter bamboo shoot shell | |
| CN110257440A (en) | Improved method for the second generation ethyl alcohol production by lignin fiber cellulosic biomass | |
| JP4886074B2 (en) | Biotreatment saccharification pretreatment method and saccharification method using the pretreatment method | |
| KR101238932B1 (en) | Manufacturing method of biobutanol from lignocellulosic biomass | |
| JP2009022165A (en) | Method for producing ethanol | |
| WO2011125992A1 (en) | Method for treating plant biomass, method for producing saccharide from plant biomass, and method for producing alcohol and/or organic acid from plant biomass | |
| CN107513545A (en) | A kind of method for strengthening abandoned biomass hydrothermal pretreatment using lignin-degrading bacteria | |
| CN102864180A (en) | Method for simultaneously preparing ferulic acid, xylo-oligosaccharides and ethanol from spent grains | |
| Liu et al. | Bacterial Cellulose Production by Acetobacter xylinum CGMCC 1.2378 Using Coconut Shell Acid Hydrolysate as Carbon Source | |
| CN105331641A (en) | Method for preparing succinic acid by using water hyacinth as fermentation raw material | |
| JP2012085544A (en) | Method for saccharifying herb-based biomass | |
| CN109097503A (en) | A method of inosite is prepared by bamboo shoots raw material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20110214 |
|
| R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20140401 Year of fee payment: 3 |
|
| R154 | Certificate of patent or utility model (reissue) |
Free format text: JAPANESE INTERMEDIATE CODE: R154 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20140401 Year of fee payment: 3 |
|
| R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20140401 Year of fee payment: 3 |
|
| LAPS | Cancellation because of no payment of annual fees |