JP4650314B2 - Biosensor electrode section - Google Patents

Description

The present invention relates to a biosensor electrode part. More specifically, the present invention relates to a biosensor electrode portion in which a sample solution is introduced onto an electrode using surface tension.

When the biosensor electrode part has a counter electrode other than the working electrode or a counter electrode and a reference electrode arranged on the same surface of the planar substrate , (1) Directly drop the measurement sample on the electrode or on the electrode substrate Measurement was performed using a structure in which a spacer having a groove was disposed on the cover, and a cover provided with air holes was further disposed thereon. In the method (1), there is a problem that it takes time and labor from sampling to dripping. On the other hand, the method (2) has an advantage that the measurement sample is guided directly onto the electrode, so that time and time are not taken. On the other hand, it has the disadvantage that a complicated process is required in the production of the biosensor electrode part , such as the need to install air holes. Further, since the electrodes are provided on one plane, an amount of sample solution covering the surface of these electrodes is required for accurate measurement.
Japanese Patent Laid-Open No. 1-291153

On the other hand, in order to further reduce the amount of the sample, a biosensor electrode portion in which the electrodes have a facing structure has been proposed. Such a biosensor electrode portion has a structure in which electrodes are provided on the surfaces of two planar substrates, respectively, and a spacer made of a resist layer, an adhesive layer, or the like is sandwiched so that these electrodes face each other. By adopting the facing electrode structure, the distance between the electrodes, that is, the distance between the substrates can be reduced as much as possible, so that the sample volume can be further reduced. It has become very efficient.
USP 6,071,391

  However, the high-efficiency electrode reaction in the planar electrode facing structure proposed in Document 2 is limited to the two-electrode method composed of a combination of a working electrode and a counter electrode that can utilize the characteristics of the facing. Therefore, when there are more types of electrodes than the two-electrode method, for example, the three-electrode method composed of a combination of a working electrode, a counter electrode, and a reference electrode, one electrode is provided on one substrate and the other substrate is provided. Two electrodes are provided and bonded with a spacer so that they face each other, but such a structure is similar to the reaction between two electrodes provided in the same plane shown in Patent Document 1. In addition, there is a disadvantage that the efficiency is remarkably lowered.

  Also, in these biosensor electrode parts, a resist layer or an adhesive layer, which serves as a spacer, is formed by printing, etc., so that the thickness of those layers varies depending on slight differences in solvent concentration, humidity, temperature, etc. As a result, there was a problem that the sample volume was changed and the reproducibility was lowered. That is, the improvement of the production yield in order to overcome these problems has been a major issue.

The object of the present invention is to enable the amount of measurement sample to be kept to the minimum necessary without reducing the measurement sensitivity even in the biosensor electrode portion having not only two electrodes but also more electrodes, and further manufacturing. The aim is to provide a product with improved yields.

The present invention is a biosensor electrode portion composed of two or more convex curved plate members, of which at least two members are made of a conductor constituting the electrode, and are defined such that the convex curved surfaces face each other. This is achieved by a biosensor electrode section that is arranged with a sample liquid introduced onto the electrode by surface tension acting between them.

Since at least two convex members are used, even in the case of the bipolar method, the amount of the sample is further reduced compared to the conventional biosensor electrode part. Specifically, the sample volume required for detection is regulated to 1 μL or less. In addition, even with three or more electrodes or two or more electrode systems, it is possible to reduce the amount of the sample without reducing the measurement sensitivity. In addition, since the distance between the electrodes is accurately defined by the spacer, the manufacturing yield can be improved.

The biosensor electrode part is composed of two or more convex curved plate members. Of these, at least two members are made of a conductor constituting an electrode , and examples of the conductor include carbon, silver, silver / silver chloride, platinum, gold, nickel, copper, palladium, titanium, iridium, lead, tin oxide, Platinum black or the like is used. Here, as the carbon, carbon nanotubes, carbon microcoils, carbon nanohorns, fullerenes, dendrimers, or derivatives thereof can be used. In addition, the other members may be the above-described conductors or may be electrically insulating materials, for example, plastics such as acrylic, polyimide, polystyrene, and polyethylene terephthalate, biodegradable materials, and paper.

Here, the convex curved surface of the convex curved plate member is not only a curved plate plate, but also a cylindrical, cylindrical, crescent column, semi-column, elliptical column, fan column, or side column convex shape of at least one curved column. Part . When a columnar object is used, a columnar body having a spherical end is preferably used from the viewpoint of facilitating sample introduction.

2 or more convex curved plate member is a convex curved surface of each convex curved plate member is arranged at a prescribed interval so as to face. With this arrangement, the measurement sample solution can be introduced into the biosensor electrode part by the surface tension acting between the two. Here, it is also conceivable to construct an electrode system including three or more electrodes by arranging three or more planar plate-like members at regular intervals. However, in the case of a combination of plate-like members, it is necessary to narrow the width of the plate and make the distance between the electrodes as small as possible in order to smoothly introduce the sample liquid due to surface tension. Unlike the case where two plates are used at the center of the plate, the surface tension does not work strongly at both ends, while a strong surface tension works between adjacent plates. There is a tendency to be slightly damaged. On the other hand, since the convex member is used in the present invention, it is possible to avoid such a decrease in surface tension.

The electrode may be a two-pole method formed with a working electrode and a counter electrode or a three-pole method formed with a working electrode and a counter electrode, a reference electrode, or an electrode method with more poles. Moreover, you may be comprised by 2 or more sets of electrode systems. For example, the three members can be arranged to face each other, or arranged so that a part of the side surface of the convex curved plate member made of a plurality of conductors is opposed to the periphery of the cylinder or column made of the conductors. You can also As described above, in the biosensor electrode part according to the present invention, since two or more convex members are used, even in the case of the bipolar method, the sample amount can be further reduced compared to the conventional biosensor electrode part. Makes it possible to regulate the sample volume required for detection to 1 μL or less, and to reduce the amount of the sample without degrading the measurement sensitivity even in the case of three or more electrodes or two or more electrode systems. be able to.

In this way, the electrodes have a facing structure in which the electrodes are three-dimensionally arranged relative to each other. In order to form such a structure, each convex curved member is joined to a support, and the support is used as a spacer. Fixed. In addition to the case where only one convex curved member is joined to the support, it is also possible to join a plurality of members. In the latter case, when forming a biosensor array in which a large number of biosensor electrode portions described later are arranged. , The density of the biosensor electrode part can be increased. In addition, since the distance between the electrodes is accurately defined by the spacer, the sample volume can be precisely defined, and the manufacturing yield can be greatly improved.

  Although it does not specifically limit about what is joined to a nonelectroconductive member as a support body, About the thing joined to a conductive member, it is preferable that it consists of a conductive material similarly. The conductive member and the conductive support may be formed integrally. As a spacer for fixing the support, plastic such as acrylic, polyimide, polystyrene, polyethylene terephthalate, biodegradable material, paper, etc. An insulating material is used. At this time, the support constitutes a terminal connected to the measuring device. The spacer also acts as a stopper for defining the sample volume. The stopper can also be formed by rolling a columnar body that forms an electrode instead of a spacer.

  A reagent layer (electrode reaction part) can be formed on the electrode. The reagent layer is formed by a dip (immersion) method, a dispenser method, or a screen printing method, and the immobilization of the reagent layer on the electrode surface or the substrate surface can be performed by an adsorption method with drying or a covalent bond method.

  Examples of the reagent disposed in the electrode reaction part include those containing glucose oxidase (GOD) which is an oxidase and potassium ferricyanide as a mediator when configured for blood glucose measurement. When the reagent is dissolved by the blood, the enzyme reaction is started. As a result, potassium ferricyanide coexisting in the reaction layer is reduced and potassium ferrocyanide, which is a reduced electron carrier, is accumulated. The amount is proportional to the substrate concentration, ie the glucose concentration in the blood. The reduced electron carrier accumulated for a certain time is oxidized by an electrochemical reaction. An electronic circuit in the measurement apparatus main body, which will be described later, calculates and determines a glucose concentration (blood glucose level) from the anode current measured at this time, and displays it on a display unit arranged on the surface of the main body.

  In addition, a surfactant and lipid can be applied to the periphery of the sample introduction port or blood collection port and to the surface of the electrode part or reagent layer (electrode reaction part). Alternatively, these surfactants and lipids may be included in the reagent described in the previous section. By applying a surfactant or lipid, the sample can be moved smoothly.

  In the biosensor electrode section described above, the movement of the sample liquid is largely due to the surface tension acting between the plates, but in addition to this, the movement of the sample liquid is caused by causing a potential difference between the electrodes. It is also possible to use a liquid feeding system (Japanese Patent Laid-Open No. 2005-199231) using electrowetting that enables smooth progress. The liquid feeding system by electrowetting uses the principle that adsorption of positive ions becomes dominant when the potential is changed to a negative potential, and adsorption of negative ions becomes dominant when the potential is changed to a positive potential. The liquid feeding system to which electrowetting is applied has an excellent effect of realizing smooth liquid feeding of the sample liquid without requiring the application of the above-described surfactant or the like.

  In the biosensor electrode part provided with the reagent layer filled with the above blood collection, the blood collection and the reagent react when the blood collected from the blood collection port comes into contact with the reagent layer in the electrode part. This reaction is monitored, for example, as an electrical change in the electrode.

  In addition, a plurality of biosensor electrode portions of the present invention can be arranged and used as an array. Also according to such an embodiment, it is possible to simultaneously measure multiple items of sample solutions.

  The biosensor electrode portion having the above-described configuration can also be used with a puncture needle for collecting body fluid from the skin of the subject. Since it is necessary to puncture the subject, the puncture needle is preferably sharp and strong enough to withstand this, and is preferably a thin puncture needle in order to suppress pain during puncture. Specifically, a 21-33 gauge thing by Terumo company is used. The puncture needle may be a hollow needle or a rod-like needle as long as it can penetrate the subject's skin.

  The biosensor electrode unit used together with the puncture needle is preferably subjected to a series of operations of puncture, blood collection, and measurement by a measurement device having a puncture drive. In this case, for example, with respect to puncture driving, it is desirable to have a mechanism in which the needle passes through the electrode part of the biosensor and breaks through the skin of the subject, and a mechanism that quickly returns to the original position immediately after puncturing.

As a measurement device for a needle-integrated biosensor, an instrument that ensures operability and durability for repeated and reliable measurement using a biosensor electrode section and is easy to carry is used. The measuring device provided has a biosensor electrode part inserted vertically so that the puncture needle can pass vertically into the sample liquid introduction part of the biosensor electrode part at the bottom, and the terminal of the biosensor electrode part is connected to the connector of the measuring apparatus. The connection is ready for measurement, and then the preparation for measurement is completed by pulling the trigger to give the puncture drive, and then the puncture / blood sampling / measurement sequence is performed by pressing the puncture start button switch. A system that automatically operates and finally obtains the measurement result is used.

An example of the structural features of the measuring device will be described in more detail. In this measurement apparatus, the puncture needle drive unit and the measurement apparatus unit are integrated, and the puncture needle drive unit includes a trigger unit, a puncture start button unit, and a drive unit using an elastic body such as a spring. The puncture needle is installed in the puncture device for each measurement. On the other hand, for the measuring device section, the sensor introduction section, the connector, the electrochemical measurement circuit, the memory section, the operation panel, the measurement section that measures the electrical values at the electrodes of the biosensor, and the display that displays the measurement values at the measurement section Further, a radio wave, for example, Bluetooth (registered trademark) can be mounted as a wireless means. With such a slide structure, the puncture drive is received while the biosensor electrode portion is securely held, so that the strength of the entire measuring apparatus can be increased. Further, when the biosensor electrode unit is composed of three columns, it is possible to adopt a bilaterally symmetric structure, in which case the three terminals of the biosensor electrode unit detector determine the position of each terminal. It is possible to provide a mechanism that can be connected to the measuring device without any problem.

  The puncture drive of the measuring device is preferably a mechanism that returns quickly after tapping the upper part of the needle-integrated biosensor in the vertical direction, and preferably has a mechanism that can adjust the depth at which the skin of the subject is punctured.

  The measuring device must have voice guidance and voice recognition functions for visual impairment caused by diabetes, measurement data management functions using the built-in radio clock, communication functions for medical data such as measurement data, and charging functions. Can do.

  The electrochemical measurement method in the measurement unit of the measurement apparatus is not particularly limited, and potential step chronoamperometry, coulometry, cyclic voltammetry, or the like can be used.

As described above, the needle-integrated biosensor electrode section of the present invention does not limit the user, that is, can handle universal projects.

The biosensor electrode portion of the embodiment according to the present invention will be described in detail with reference to the drawings. However, the present invention is not limited to the following examples unless it exceeds the gist.

Example 1
FIG. 1 is a diagram showing a configuration example and a usage example of a biosensor electrode unit according to the present invention. a) i) shows a side view of the biosensor electrode unit 9. Three curved plate-like electrodes 1 forming a convex surface are each partially joined to a columnar support body 3 at an upper portion, and each support body 3 is fixed to a spacer 2 so that each curve forming a convex surface is formed. The face plate electrodes 1 are opposed to each other at equal intervals to constitute a biosensor electrode portion. The spacer 2 also functions as a stopper 4 that stops the sample liquid 5 from rising. The biosensor electrode portion 9 configured in this way is shown above the sample solution 5. a) ii) is a cross-sectional view taken along the line AA ′ before being immersed in the sample solution 5. The three curved plate-like electrodes 1 are shown to be joined to the cylindrical support 3 respectively, and the three curved plate-like electrodes 1 are arranged at equal intervals due to the presence of spacers. A space formed between the electrodes serves as a sample transport path (electrode reaction part) 13. b) shows a state immediately after the biosensor electrode portion 9 enters the liquid surface of the sample liquid 5 and before the sample liquid 5 is introduced into the sample transport path 13 by the surface tension acting between the three curved plate-like electrodes. The state of is shown. c) i) shows a state in which the sample liquid 5 is removed from the liquid surface of the sample liquid 5 after filling the sample transport path 13 of the biosensor electrode unit 9. Here, the sample liquid 5 introduced into the sample transport path 13 can stably remain only between the three curved plate-like electrodes 1 on which surface tension acts. c) ii) shows a plan view of the biosensor electrode unit 9, and each support 3 is fixed to the spacer 2. c) iii) is a sectional view taken along line BB ′ shown in c) i). Here, a state is shown in which the sample liquid 5 is stably held in the sample transport path 13 by the surface tension acting between the three curved plate-like electrodes 1. c) iv) is a cross-sectional view taken along the line CC ′ shown in c) ii). Here, the state in which the sample solution 5 fills the sample transport path 13 of the biosensor electrode unit 9 is also shown. In this figure, the biosensor electrode portion using the curved plate-like electrode 1 having three convex surfaces is shown as an example, but the biosensor electrode using the curved plate-like electrode having more convex surfaces than this is shown. it may be a part or curved plate-like electrodes 1 do not constitute the electrodes one combination such curved plate forming the convex surface of the biosensor electrode portions forming the two convex. When at least two of the curved plates form electrodes, a support (support) that supports them serves as a terminal, so that the spacer 2 is preferably an insulator. If it is this form, the 3 electrode method can be used by making each curved-surface board which comprises three convex surfaces into a single electrode, and can obtain an electrode reaction efficiently three-dimensionally. Moreover, you may perform multiitem measurement because the curved-surface board which comprises a convex surface comprises a some working electrode, respectively.

Comparative Example 1
FIG. 2 is a diagram illustrating a configuration example of a biosensor electrode unit including a convex curved plate member and a concave curved plate member . The three curved plates are arranged so that the convex surfaces of the three curved plate-like electrodes 1 face outward with respect to the center of the biosensor electrode portion, and face the convex portions of the curved plate-like electrodes 1. The concave surfaces of the electrode 1 are arranged so as to face inward with respect to the center. a) i) is a biosensor electrode in which the spacer 2 that defines the distance between the electrodes is fixed to each of the three curved plate-like electrodes 1 and the three curved plate-like electrodes 1 that form the concave surface. A side view of part 9 is shown. The spacer 2 also acts as a stopper 4 that stops the sample liquid 5 from rising. The biosensor electrode portion 9 configured in this way is shown above the sample solution 5. a) ii) shows an AA ′ cross-sectional view of the biosensor electrode portion before being immersed in the sample solution 5. As shown here, the curved plate-like electrode 1 having three convex surfaces is disposed to face the curved plate-like electrode having a concave surface at a specified distance, and constitutes a biosensor electrode portion. Yes. Each pair of the three sets of curved plate-like electrodes 1 is arranged at equal intervals due to the presence of spacers. b) shows that, immediately after the biosensor electrode portion 9 enters the liquid surface of the sample solution 5, the sample solution 5 has three sets of curved plate-like electrodes, due to the surface tension acting between the three sets of curved plate-like electrodes 1. The state before being introduced into the sample transport path 13 provided therebetween is shown. c) i) shows a state in which the sample liquid 5 is removed from the liquid surface of the sample liquid 5 after filling the sample transport path 13 of the biosensor electrode unit 9 . c) ii) shows a plan view of the biosensor electrode unit 9, and each support 3 is fixed to the spacer 2. c) iii) is a sectional view taken along line BB ′ shown in c) i). Here, the state in which the sample liquid 5 is stably held in the sample transport path 13 by the surface tension acting between the three sets of curved plate-like electrodes 1 is shown. c) iv) is a sectional view taken along the line CC ′ shown in c) i). Here, the state in which the sample solution 5 fills the sample transport path 13 of the biosensor electrode unit 9 is also shown. With this structure, no sample liquid is introduced because surface tension does not act on the inside of the curved plate-like electrode 1 having three convex surfaces.

Example 2
FIG. 3 is a diagram showing still another configuration example and one usage example of the biosensor electrode unit according to the present invention. The biosensor electrode portion shown in this figure is arranged with a prescribed distance between two cylindrical electrodes 1 instead of the curved plate-like electrodes 1 forming the three convex surfaces shown in FIG. The sample liquid is introduced between the electrodes by using the surface tension acting between the curved surfaces forming the convex surfaces which are the side surfaces of the two cylinders. a) shows a side view of the biosensor electrode section 9. Here, a thick cylindrical column at the bottom constitutes the electrode 1, and an upper portion connected to the cylindrical column is a cylindrical support 3 that is thinner than the lower portion, and serves as a terminal for sending an electrode signal to a measuring instrument. Playing a role. In this case, the material of the upper and lower cylinders may be the same or different. As in FIG. 1, a spacer 2 is provided for strictly defining the spatial arrangement of these two cylinders, that is, the distance between the electrodes. The spacer 2 is provided with a through hole for arranging two cylindrical terminals. b) i) is a perspective view, and there is a sample solution 5 below the biosensor electrode part 9, and a cross-sectional view along the broken line AA ′ shown therein is shown in b) ii). Yes. Here, it can be seen that the two cylindrical electrodes 1 are arranged close to each other at a distance where they do not contact each other. A space between these electrodes is a sample transport path 13. c) shows a state in which the biosensor electrode unit 9 is in contact with the sample solution 5. d) shows a state in which the sample liquid 5 is introduced by the surface tension acting between the two cylindrical electrodes 1 from the sample introduction port 12 of the biosensor electrode section 9 to the sample transport path 13, in i) a perspective view, and ii) Fig. 3 is a front longitudinal sectional view of i), and Fig. 3 is a sectional view taken along line BB 'of i). As can be seen in these figures, the sample liquid 5 is introduced between the two lower cylindrical electrodes, but the surface tension does not reach the thin cylinder forming the terminal above it, so that it is more than necessary. The sample solution is not introduced. That is, this cylindrical structure is a stopper 4 for defining the volume of the sample solution. In order to define a further constant volume of the sample solution 5, it is effective to first make the lower surface of each cylinder in contact with the sample solution 5 hydrophobic so that, for example, if the sample solution 5 is an aqueous solution, it repels it. It is. Examples thereof include tetrafluoroethylene and the like. Here, the electrode portion 1 may be formed only in a range where the surface tension of the sample liquid reaches between the two cylinders, or the entire surface of the cylinder may constitute the electrode. In this case, in addition to the two-electrode method, for example, it is possible to provide a two-electrode method on one cylinder and use the three-electrode method. In the latter case, the most efficient electrode response can be obtained by using a working electrode and a reference electrode for one cylinder, and a counter electrode that requires a larger area than the other electrodes for one cylinder.

Comparative Example 2
FIG. 4 is a diagram showing still another configuration example and one example of use of the biosensor electrode unit including the convex curved plate member and the concave curved plate member. The curved plate-like electrodes 1 having three concave surfaces are arranged at equal intervals around the cylindrical electrode 1 having a spherical tip, forming a biosensor electrode portion. a) i) shows a side view of the biosensor electrode portion 9 disposed above the sample solution 5, and also shows a cross-section AA ′ shown in a) ii) a) i). The figure is shown. In a) i), it is shown that the cylinder 1 arranged at the center is narrower in the lower part where the spacer 2 is fitted. The narrowed portion is obtained by collecting a predetermined volume of the sample liquid 5 by the surface tension acting between the surfaces of the curved plate electrode 1 and the cylindrical electrode 1 having three concave surfaces, and does not raise the sample liquid any more. It plays the role of the stopper 4 for. b) shows a state in which the biosensor electrode unit 9 is immersed in the liquid surface of the sample solution 5; c) i) shows a state after the sample solution 5 fills the sample transport path 13 of the biosensor electrode unit 9; The state which removed from the liquid level of the liquid 5 is shown. c) ii) shows a longitudinal sectional view of the center line shown in c) i). As shown in this figure, it can be seen that the sample solution is filled only between the curved plate-like electrode 1 and the cylindrical stopper. c) iii) shows a cross-sectional view along B-B ′ shown in c) i). A state is shown in which the sample solution is quantitatively introduced only into the three curved plate-like electrodes 1 and the surface portion of the cylinder facing the portion.

Example 3
FIG. 5 is a diagram showing still another configuration example and one usage example of the biosensor electrode unit according to the present invention. Unlike FIG. 3 , three cylinders with rounded tips are arranged at equal intervals with a specified distance, and the thickness of the cylinder is the same up to the spacer 2. The electrode 1 is fixed to the spacer 2 because the portion extending to is thinner than the lower electrode portion 1. a) As shown in i), the spacer 2 serves as a stopper 4 for the sample solution. a) ii) is an assembly drawing of the biosensor electrode unit 9, and the biosensor electrode unit can be easily and reliably assembled by passing the cylinders 1 having different thicknesses through the spacer 2 on the upper and lower sides. The terminal 3 is easily passed through the spacer 2 by rounding the tip. b) shows an i) perspective view and ii) AA ′ cross-sectional view of the biosensor electrode section 9, and b) a sample solution 5 is placed below i). c) shows a state in which the biosensor electrode unit 9 is in contact with the sample solution 5, and d) shows a spacer 2 in which the sample solution 5 serves as the stopper 4 of the biosensor electrode unit 9 after the biosensor electrode unit 9 is pulled up. A state in which the sample transport path 13 is reached is shown. In the case of this biosensor electrode part , since the sample solution 5 may enter between the spacer 2 and the terminal 3, the property of the solvent of the sample solution 5, for example, when it is water-soluble, at least the terminal 3 and the spacer 2 are It is preferable to take a method such as using a hydrophobic material for the contacting interface. Here, d) iii) shows the CC ′ cross section shown in d) i). As shown in this figure, in the biosensor electrode section 9 of the present invention, the three cylindrical electrodes 1 are adjacent to each other with a certain distance by the spacer 2, so that the sample solution 5 and the three cylindrical electrodes 1 The surface tension acting between the surfaces is generated, and an excellent effect is obtained such that a constant volume of the sample liquid 5 can be held.

Example 4
FIG. 6 shows an example of a biosensor array using the biosensor electrode section shown in FIG. A biosensor in which the biosensor electrode portions 9 shown in FIG. 5 are arranged in 12 rows and 8 columns at equal intervals, these are fitted into through holes provided in the flat substrate 11, and 96 biosensor electrode portions are provided. An array is shown. In a), the biosensor electrode portions 9 are regularly arranged by the flat substrate 11, and in b) a side view thereof is shown. With such an embodiment, it is possible to measure multiple sample liquids simultaneously.

Example 5
FIG. 7 is a diagram showing still another configuration example and one usage example of the biosensor electrode unit according to the present invention. The biosensor electrode unit 9 shown in FIG. 5 is that the tip of the cylinder forming the electrode 1 is a flat surface, and that the biosensor electrode unit is configured by fitting the narrowed portion of the terminal 3 into the spacer 2. Is different. The configuration in which the narrowed portion of the terminal 3 is fitted in the spacer 2 makes it possible to easily hold the terminal 3 in the spacer 2, so that the assembly is easy and the yield can be improved. is there. The details of the assembly process are shown in a) ii) by the AA 'cross-sectional view of a) i). The perspective view after assembly is shown in b) i) and the BB' cross-sectional view in b) ii. ) Shows a cross-sectional view along CC ′ in b) iii). Further, a perspective view of the biosensor electrode part 9 into which the sample solution 5 is introduced is shown in d) i), a DD ′ sectional view in d) ii), and a EE ′ sectional view in iii).

Example 6
FIG. 8 shows another example of a biosensor array. In a), the biosensor electrode parts 9 are arranged in 12 rows and 8 columns at equal intervals, these are fitted into through holes provided in the flat substrate 11, and 96 biosensor electrode parts are provided. Is shown. The biosensor electrode unit 9 used here has the same structure as the biosensor electrode unit shown in FIG. 7, but the terminal 3 portion is formed in a through hole provided in the flat substrate 11 as shown in b). It has a shape where the tip is narrowed so as not to go deeper than specified. Moreover, in order to make it easy to insert the terminal 3 into the flat substrate 11, the tip is rounded.

Example 7
FIG. 9 is a diagram illustrating a configuration example and a usage example of the biosensor electrode unit for multi-item simultaneous measurement. Three sets of biosensor electrode portions shown in FIG. 3 are arranged at equal intervals with a prescribed distance on the side surface of one cylindrical electrode 1. a) is a side view of the biosensor electrode unit 9, b) i) is a perspective view thereof, and ii) is a cross-sectional view taken along line AA ′, and b) a sample solution 5 is placed below i). Yes. c) shows a state in which the biosensor electrode part 9 is in contact with the sample solution 5; d) i) shows a sample in which the sample solution 5 reaches the stopper 4 of the biosensor electrode part 9 after the biosensor electrode part 9 is pulled up. A state of reaching the conveyance path 13 is shown. d) ii) is a cross-sectional view taken along line BB ′ shown in d) i). As shown in this figure, the area of contact with the sample solution 5 is larger in the thick cylindrical electrode 1 arranged at the center than in each pair of thin cylindrical electrodes 1, so that this can be used as a common counter electrode. There is a feature that four sets of three electrode systems can function. In addition, in order to accurately define the volume of the sample solution 5 as in FIG. 4, the lower surface of these cylindrical electrodes 1 is made of a material having a poor affinity with the sample solution, thereby effectively attaching the sample solution. Can be suppressed.

Example 8
FIG. 10 is a diagram showing still another configuration example and one usage example of the biosensor electrode unit according to the present invention. The biosensor electrode unit 9 shown in FIG. 7 is different from the biosensor electrode unit 9 in that the biosensor electrode unit is configured by passing the cylindrical terminal 3 through the spacer 2 and the shape of the electrode 1. a) i) is a view showing the process of assembling each member, ii) is a plan view of the biosensor electrode part excluding the spacer 2, iii) is a bottom view thereof, and iv) is a plan view of the completed biosensor electrode part . FIG. V) shows the bottom view. Since the three curved surface portions forming the convex surface of each member face each other, the sample liquid 5 is transported in the same manner as in FIG. b) shows a state in which the biosensor electrode unit 9 is in contact with the sample solution 5, and c) shows a sample transport path from which the sample solution 5 reaches the stopper 4 of the biosensor electrode unit 9 after the biosensor electrode unit 9 is pulled up. It shows how it reaches 13. d) shows a bottom view of the biosensor electrode part excluding the spacer 2 into which the sample solution has been introduced.

Example 9
Figure 11 is a diagram showing an example of using both the puncture needle biosensor electrode unit shown in FIG. 10. As shown in a), the puncture needle is arranged and used so that it can move vertically between the supports (terminals) of the biosensor electrode part . If the shape of the biosensor electrode part shown in FIG. 10, unlike the biosensor electrode part shown in FIGS. 1 to 9, the three terminals 3 are arranged at a distance from the electrode 3, There is a feature that the puncture needle 15 can be arranged in the center of the space. Further, this shape is characterized in that the stepped portion between the terminal and the electrode also functions as the stopper 4 for the sample solution 5. a) i) is a side view of the biosensor electrode unit 9 in which the puncture needle is arranged so that the center between the supports (terminals) of the biosensor electrode unit can be moved vertically, and ii) is the spacer 2 of i). The plan view removed, iii) is a bottom view excluding the spacer 2 of i). As is clear from these drawings, the puncture needle 15 provided with the puncture needle support 27 is arranged so as to be able to penetrate the sample transport path 13 of the biosensor electrode unit 9. b) is before the puncture needle 15 into the skin 7 of the patient is punctured through the biosensor electrode in the unit state, c) the state in the puncture, d) in after puncturing, the puncture needle 15 is the sample transfer path In the state where the blood sample 8 which is moved upward from 13 and the blood sample 8 which is the sample solution 5 is conveyed from the puncture blood collection port 14 to the electrode reaction unit 13, and e), the measurement of the blood sample component conveyed to the electrode reaction unit 13 is performed. Each status is shown.

Example 10
FIG. 12 is a diagram illustrating an example of use of the biosensor electrode unit illustrated in FIG. 11 including a measurement device. In a), a pen-type measuring device 16 with a puncture drive is shown. The measuring device 16 is provided with a mounting portion 24 for the needle 15 necessary for puncturing and a mounting portion 21 for the biosensor electrode unit 9 both below the measuring device 16. An operation panel 17 is provided at the center, and a trigger 19 for puncture is provided at the top. b) a state where the puncture needle 15 and the biosensor electrode unit 9 are attached to the measuring device 16, c) a state where the trigger 19 for puncture is pulled, and d) a biosensor which is the puncture blood collection port 14 In the state where the tip of the electrode unit 9 is placed on the skin 7 of the subject and a series of operations of puncture, blood collection and measurement are performed, e), the measurement of blood components is completed and the trigger unit is pushed in Thus, in the state in which both the puncture needle 15 and the biosensor electrode unit 9 are removed from the measuring device 18, f), the state in which the attached cap 25 is attached is shown to remove the measuring device 18 after use. .

Example 11
FIG. 13 is a diagram showing an example of packaging of the biosensor electrode unit shown in FIG. As shown in a), the puncture needle 15 and the biosensor electrode part 9 are enclosed in separate compartments in the package 22 and hermetically packaged by a removable film-like lid (packaging film) 23. As an example of its use, first, as shown in b) to c), the packaging film 23 is peeled off, and the puncture needle 15 is attached to the measuring device 16. Next, as shown in d) to f), the biosensor electrode unit 9 is attached. After the measurement is completed, the used puncture needle 15 and the biosensor electrode unit 9 are returned to the original package 22 as shown in g), covered with a lid for preventing infection as shown in h), and then discarded together with the package. It is preferable to do. Thus, by making the puncture needle 15 and the biosensor electrode unit 9 used in one measurement into one set of the package 22, both operability and hygienic safety after use can be provided. For example, in order to prevent such a package 22 from being disturbed even if it is carried, it is possible to use about 10 sticks as a group as shown in i), and separate and use each package 22 at the time of use. .

Example 12
FIG. 14 is a diagram showing an example of a biosensor electrode unit according to the present invention using a liquid feeding system to which electrowetting is applied. In this figure, a liquid feeding system in a state where three cylinders are arranged close to each other is shown. Each cylinder is provided with a conductor that constitutes an electrode having a prescribed area with the insulating portion 31 interposed therebetween, and a working electrode 28 as a driving electrode or a counter electrode 29 as a reference electrode, a non-electrode, depending on the potential operation. It can be any part 30. The two cylinders shown below have the same pattern of the electrode and the insulating portion 31, and are different from the pattern of the upper one cylinder. By utilizing this deviation, liquid feeding by electrowetting becomes possible.

For example, in a), a constant volume of the sample liquid 5 is stagnated between three cylinders due to surface tension. In this state, no electric potential is applied to any conductor in contact with the sample solution 5. Next, one of the lower cylinders in contact with the sample liquid is the working electrode 28, and the part of the upper cylinder in contact with the sample liquid is the counter electrode 29, and the working electrode 28 has a potential of about −0.7V. By applying, the sample solution 5 moves to the position shown in b) according to the arrow direction (hereinafter, the potential application direction is shown). Here, as shown in b), the sample solution 5 is moved to the position shown in c) by applying a potential to the two conductors. When the sample liquid 5 moves to the inside of the liquid supply flow path, when a new sample liquid is introduced as shown in d) and a potential is applied, the two sample liquids 5 are placed in the liquid supply flow path as shown in e). Mixed in. For example, when one of the two sample solutions 5 before mixing here is glucose oxidase (GOD) aqueous solution and the other is blood collection as a biological sample, the mixture sample solution 5 of e) is included during blood collection. Hydrogen peroxide is generated according to the glucose concentration. Therefore, when a potential of +0.7 V is applied to the working electrode in this state, a current value corresponding to the concentration of hydrogen peroxide can be obtained, and this system makes it possible to measure a blood glucose level during blood collection . Here, glucose and hydrogen peroxide are completely decomposed by application of a measurement potential.

  Next, f) to h) show a state in which a new reagent solution is mixed with the previously introduced reagent solution by the same method. If lactate oxidase is contained in the new sample solution used for these operations, after these sample solutions 5 are mixed, the peroxidation according to the concentration of lactic acid contained in the blood sample from which the glucose concentration was measured first. Since hydrogen is produced, the lactic acid concentration can be measured in the same manner as glucose. Thus, by introducing the sample liquid 5 between the cylinders having three conductive portions, the sample liquid 5 can be mixed and mixed. Further, for example, by performing the potential operation as shown in i) from the state of h), the sample solution can be dispensed.

Example 13
FIG. 15 is a diagram showing another example of the biosensor electrode unit according to the present invention using a liquid feeding system to which electrowetting is applied. Since the pitch of the conductors of the three cylinders shown here are different from each other, it is possible to supply liquid more accurately than the method shown in FIG. 14 by finely controlling the conductors arranged in the three cylinders. It becomes. This figure shows a state where three types of sample liquids 5 are independently fed at regular intervals.

Example 14
FIG. 16 is a diagram showing still another example of the biosensor electrode unit according to the present invention using a liquid feeding system to which electrowetting is applied. The four cylinders shown in this figure are arranged at the same distance between the top and bottom, respectively, and by setting the two conductors on the top and bottom to the same polarity, higher driving force can be achieved. There are features that can be obtained. In any of FIGS. 14-16, the code | symbol 3 in drawing is used not only as a support body but as a terminal.

Example 15
FIG. 17 shows still another example of a biosensor array. Basically, two convex curved plate-like electrodes 1 are joined to the columnar support 3 and arranged opposite to the convex curved plate electrode 1 joined to the other cylindrical support 3. A biosensor array having a two-electrode biosensor electrode section is constructed. a) is a bottom view showing a state in which the two-electrode biosensor electrode portions 9 are arranged in 10 rows and 10 columns at equal intervals in the vertical and horizontal directions, and these are fixed to the flat substrate 11 via the support 3. b) shows an AA ′ section shown in a), and c) shows a BB ′ section. In such an embodiment, the sample solution is introduced into the sample transport path by the surface tension acting between the two convex curved plate members, and since it is in the form of an array, it is possible to measure multiple items of sample solution simultaneously. it can. Further, as compared with the biosensor array 10 shown in FIG. 6 or FIG. 8, by providing a plurality of electrodes on one columnar body, there is an excellent effect that the biosensor electrode portion 9 can be integrated in a small area.

Here, when the two electrodes (conductors) provided on the cylindrical support 3 are both used as counter electrodes with respect to the electrodes facing each other, the cylindrical support 3 is made of a conductive material. One terminal can be used after being joined to two electrodes. On the contrary, whether these two electrodes are used as different polarities or both are used as counter electrodes, the configuration in which each electrode is wired to each terminal, that is, the two electrodes are Each may be independent. In such a case, the columnar support 3 is made of an electrically insulating material. In such a biosensor array, the two convex curved plate members need to face each other accurately, so that each columnar support 3 needs to be fixed and arranged on the flat substrate 11 in an accurate orientation. Therefore, preferably, a mechanism for positioning that defines the orientation of each biosensor electrode portion 9 is provided in the through hole of the flat substrate 11 and / or the electrode support 3, for example, a mechanism such as a relationship between a key and a key hole. . The contents described in this paragraph also apply to the biosensor array described below.

Example 16
FIG. 18 shows still another example of a biosensor array. Two convex curved plate-like electrodes 1 are basically joined to the columnar support 3, and opposed to the convex curved plate electrodes 1, 1 joined to the other two cylindrical support 3. A biosensor array is shown having biosensor electrode portions constructed by placement. a) is a bottom view showing a state in which the biosensor electrode portions 9 are arranged in a honeycomb state and these are fixed to the flat substrate 11 via the support 3. b) shows an AA ′ section shown in a), and c) shows a BB ′ section. In such an embodiment, the sample liquid is introduced into the sample transport path 13 by the surface tension acting between the three convex curved plate-like electrodes, and each biosensor electrode section 9 is a maximum of a three-electrode system. Two sets of measurements in a two-electrode system can be performed by using one measurement electrode or a common counter electrode, and a multi-item sample solution can be measured simultaneously because of the array shape. Similarly to FIG. 17, the three electrodes provided on the cylindrical body may have the same terminal or may be independent of each other, and can be appropriately used according to the application. A positioning mechanism that defines the orientation of the biosensor electrode unit 9 may be provided in the through hole of the support 3 and the flat substrate 11.

Example 17
FIG. 19 shows still another example of a biosensor array. All of the biosensor arrays shown in this figure are arranged in a state where the density of the biosensor electrode portion is increased as compared with FIGS. In any of the figures, two or four convex curved plate-like electrodes 1 are basically joined to a cylindrical or elliptical cylindrical support 3, and the other four cylindrical supports 3 are joined. A biosensor array having a biosensor electrode portion configured by being arranged to face an electrode 1 of a convex curved plate is shown. a) is basically an elliptical columnar support 3 in which four convex curved plate-like electrodes 1 are joined so that the electrodes 1 joined to three different supports 3 are opposed to each other, and A bottom view shows a state in which cylindrical support bodies 3 in which two convex curved plate members are joined to each other are alternately arranged.

  In FIG. 19b), basically, a cylindrical support 3 to which three convex curved plate-like electrodes 1 are joined is opposed to an electrode 1 joined to three different cylindrical supports 3. The biosensor array which has the biosensor electrode part arrange | positioned in is shown with the bottom view. FIG. 19 c) shows a convex curved plate-like shape in which basically three convex curved plate-like electrodes 1 are joined to a cylindrical support 3 and joined to the other two cylindrical supports 3. The biosensor array which has the biosensor electrode part comprised by arrange | positioning facing the electrode 1 is shown with the bottom view.

  In the embodiment shown in FIG. 19, the sample liquid is introduced into the sample transport path 13 by the surface tension acting between the three convex curved plate-like electrodes, as in FIG. Two sets of measurements in a two-electrode system are possible by using a measurement with a three-electrode system or a common counter electrode, and it is of course possible to measure multiple items of sample solution simultaneously because of the array shape. Since it is arranged in a state where the density is increased, the biosensor electrode array can be made more compact. Similarly to FIG. 17, the three electrodes provided on the cylindrical body may have the same terminal or may be independent of each other, and can be appropriately used according to the application. A positioning mechanism that defines the orientation of the biosensor electrode unit 9 may be provided in the through hole of the support 3 and the flat substrate 11.

Example 18
FIG. 20 shows still another example of a biosensor array. Each of the biosensor arrays shown in this figure is provided with a convex curved plate-like electrode 1 on each end face of one rectangular parallelepiped, and a convex curved plate-like electrode provided on the short side of another rectangular parallelepiped. A number of biosensor electrode portions are configured to face the electrode 1. a) is a bottom view showing a state in which the two-electrode biosensor electrode portions 9 are arranged in 5 rows and 4 columns at equal intervals in the vertical and horizontal directions, and these are fixed to the flat substrate 11 via a support. b) shows an AA ′ cross-sectional view shown in a), and c) shows a BB ′ cross-sectional view. In such an embodiment, the sample solution is introduced into the sample transport path by the surface tension acting between the two convex curved plate-like electrodes, and is in the form of an array, so multiple sample solutions are measured simultaneously. be able to.

  FIG. 20d) shows that the biosensor electrode unit 9 is formed by arranging three rectangular parallelepiped convex curved plate-like electrodes 1 so as to face each other. A bottom view of a biosensor array that allows two sets of measurements in a two-electrode system by using a common counter electrode is shown. The biosensor electrode portions 9 are arranged in a honeycomb shape, and these are fixed to through holes provided in the flat substrate 11 via a support. In such an embodiment, the sample liquid is introduced into the sample transport path by the surface tension acting between the three convex curved plate-like electrodes and is in the form of an array, so that multiple sample liquids are measured simultaneously. be able to.

  FIG. 20e) forms a biosensor electrode portion 9 by arranging four rectangular parallelepiped convex curved plate-like electrodes 1 so as to face each other, which is measured at the maximum in a four-electrode system, or A biosensor array that enables two sets of measurements in a three-electrode system by using one counter electrode and one reference electrode in common, or three sets of measurements in a two-electrode system by using one counter electrode in common It is shown in the figure. The biosensor electrode portions 9 are arranged in a grid pattern, and these are fixed to through holes provided in the flat substrate 11 via a support. In such an embodiment, the sample liquid is introduced into the sample transport path by the surface tension acting between the four convex curved plate-like electrodes, and is in the form of an array, so that multiple sample liquids are measured simultaneously. be able to.

  FIG. 20f) forms a biosensor electrode part 9 by arranging six rectangular parallelepiped convex curved plate-like electrodes 1 so as to face each other, which is measured at the maximum in a six-electrode system, or A biosensor array that enables four sets of measurements in a three-electrode system by using one counter electrode and one reference electrode in common, or five sets of measurements in a two-electrode system by using one counter electrode in common It is shown in the figure. The biosensor electrode portions 9 are arranged in a mesh shape, and these are fixed to through holes provided in the flat substrate 11 via a support. In such an embodiment, the sample solution is introduced into the sample transport path by the surface tension acting between the six convex curved plate-like electrodes, and is in the form of an array, so that multiple items of sample solution are measured simultaneously. be able to. In addition, each biosensor array shown in FIG. 20 may have three electrodes provided on the columnar body as in FIG. It can be properly used according to the situation.

Example 19
FIG. 21 shows still another example of the biosensor electrode array. The convex curved plate-like electrode 1 provided on the end face of the Y-shaped columnar body is the convex curved plate-like electrode 1 or rectangular solid provided on the other two Y-shaped columnar body end faces. The biosensor electrode portion 9 is formed by being arranged so as to face the convex curved plate-like electrode 1 provided on the end face. a) is a side view of a Y-shaped columnar body in which a convex curved plate-like electrode 1 is provided on an end surface. The three curved electrodes 1 forming a convex surface are respectively joined to the end surfaces of the Y-shaped columnar body. b) shows an AA ′ cross-sectional view of a) and FIG.

  d) is a convex curved plate in which the convex curved plate-like electrode 1 provided on the end surface of the Y-shaped columnar body shown in a) is provided on the other two Y-shaped columnar end surfaces. The electrode 1 having a curved surface or a convex curved plate 1 provided on the end face of a rectangular parallelepiped and the convex curved plate 1 provided on the end face of a Y-shaped columnar body are opposed to the bio. The bottom view of the biosensor array in which the sensor electrode portion is fixed to the through-hole provided in the flat substrate 11 via the support is shown, and e) is provided on the end face of the Y-shaped columnar body shown in a). A biosensor electrode portion arranged so that the convex curved plate-like electrode 1 is opposed to the convex curved plate-like electrode 1 provided on the other two Y-shaped columnar body end faces, The bottom view of the biosensor array fixed to the through-hole provided in the plane board | substrate 11 via the support body is shown. In these biosensor electrode part arrays 10, each biosensor electrode part 9 is formed by arranging three convex curved members at intervals of 120 °. The biosensor electrode unit 9 can perform measurement with a maximum of three electrodes or two sets of measurements in a two-electrode system by using one counter electrode in common. If it is such an aspect, since sample liquid will be introduce | transduced into a sample conveyance path by the surface tension which acts between three convex curved-surface members, and it is an array form, it can measure many sample liquids simultaneously. . Similarly to FIG. 17, the three electrodes provided on the cylindrical body may have the same terminal or may be independent of each other, and can be appropriately used according to the application.

Example 20
FIG. 22 shows still another example of a biosensor array. The convex curved plate-like electrode 1 provided on the end surface of the cross-shaped columnar body is arranged so as to face the convex curved plate-like electrode 1 provided on the other three cross-shaped columnar body end surfaces. As a result, the biosensor electrode portion 9 is formed. a) is a side view of a cross-shaped columnar body provided with a convex curved plate-like electrode 1 on its end face. Four curved electrodes 1 forming a convex surface are joined to end faces of Y-shaped columnar bodies, respectively. b) shows an AA ′ cross-sectional view of a) and FIG.

  d) shows a biosensor electrode unit configured by arranging four cross-shaped pillars each provided with a convex curved plate-like electrode 1 on each end face shown in a) so that the electrodes 1 face each other. The bottom view of the biosensor array fixed to the through-hole provided in the plane board | substrate 11 through the support body in the shape of a mesh is shown. This biosensor electrode unit can measure at maximum four electrode systems, or two sets of measurements in a three-electrode system by using one counter electrode and one reference electrode in common, or one counter electrode in common. Three sets of measurements in a two-electrode system are possible. In such an embodiment, the sample liquid is introduced into the sample transport path by the surface tension acting between the four convex curved members and is in the form of an array, so that multiple items of sample liquid can be measured simultaneously. . e) is a cross-sectional view taken along the line C-C ′ shown in d). In such a biosensor array 10, as in FIG. 17, the three electrodes provided on the cylindrical body may have the same terminal or may be independent of each other. Can be used properly.

It is a figure which shows one structural example and one usage example of the biosensor electrode part which consists of three convex curved-surface board members. It is a figure which shows the example of 1 structure of the biosensor electrode part which consists of three convex curved plate members, and three concave curved plate members, and its usage example. It is a figure which shows one structural example and its usage example of the biosensor electrode part which consists of two cylindrical members. It is a figure which shows the example of 1 structure of the biosensor electrode part which has arrange | positioned three concave curved-surface board members around a cylindrical member, and its usage example. It is a figure which shows one structural example and its usage example of the biosensor electrode part which consists of 3 cylindrical members. It is a figure which shows an example of the biosensor array which consists of a biosensor electrode part which concerns on this invention. It is a figure which shows the other structural example of the biosensor electrode part which consists of 3 cylindrical members, and its usage example. It is a figure which shows the other example of the biosensor array which consists of a biosensor electrode part which concerns on this invention. It is a figure which shows one structural example of the biosensor electrode part which has arrange | positioned 8 cylindrical members around a cylindrical member, and its usage example. It is a figure which shows one structural example of the biosensor electrode part which consists of 3 columnar bodies, and its usage example. It is a figure which shows an example which uses the biosensor electrode part shown in FIG. 10 with a puncture needle . It is a figure which shows one usage example of the biosensor electrode part shown in FIG. 11 including a measuring apparatus. It is a figure which shows the example of 1 packaging of the biosensor electrode part shown in FIG. 11, and the attachment example to a measuring device. It is a figure which shows an example of the biosensor electrode part which consists of 3 cylindrical members using the liquid feeding system which applied electrowetting. It is a figure which shows the other example of the biosensor electrode part which consists of 3 cylindrical members using the liquid feeding system which applied electrowetting. It is a figure which shows an example of the biosensor electrode part which consists of 4 cylindrical members using the liquid feeding system which applied electrowetting. A biosensor array composed of a biosensor electrode unit provided with two convex curved plate-like electrodes on one cylindrical body and opposed to the convex curved plate-like electrode provided on another cylindrical body. It is a figure which shows an example. Bio composed of two convex curved plate-shaped electrodes provided biosensor electrode portion made so as to face a convex curved plate-shaped electrodes provided on the cylindrical body of the other two in the cylindrical body of single It is a figure which shows an example of a sensor array. Three, or four convex curved plate-shaped electrodes are provided on the columnar body of one, from the biosensor electrode portion made so as to face a convex curved plate-shaped electrodes provided on the cylindrical body of the other two It is a figure which shows three examples of the biosensor array which becomes . A biosensor electrode formed by providing a convex curved plate-like electrode on each end face of one rectangular parallelepiped and facing a convex curved plate-like electrode provided on each end face of one or more other rectangular parallelepipeds It is a figure which shows an example of the biosensor array which consists of a part. A convex curved plate electrode is provided on each end face of the Y-shaped column, and a convex curved plate electrode provided on the end face of the rectangular parallelepiped or a convex curved plate provided on the end face of the Y-shaped column. it is the electrode and the counter is a diagram showing an example of a biosensor array of the biosensor electrode portions formed by. A biosensor electrode unit provided with a convex curved plate-like electrode on each end face of the cross-shaped columnar body and opposed to the convex curved plate-shaped electrode provided on the end faces of the other three cross-shaped columnar bodies It is a figure which shows an example of the biosensor array which consists of .

1 Electrode (conductor)
2 Spacer 3 Support (terminal)
4 Stopper 5 Sample solution 6 Puncture needle 7 Finger 8 Blood collection 9 Biosensor electrode section 10 Biosensor array 11 Planar substrate 12 Sample inlet 13 Sample transport path (electrode reaction section)
14 Puncture blood collection port 15 Puncture needle 16 Measuring device 17 Operation panel 19 Trigger 20 Puncture button 21 Sensor mounting portion 22 Package 23 Lid 24 Puncture needle mounting portion 25 Cap 27 Puncture needle support 28 Working electrode (drive electrode)
29 Counter electrode (reference electrode)
30 Non-electrode part 31 Insulating part

Claims (11)

A biosensor electrode unit comprising two or more convex curved plate members, at least two of which are made of a conductor constituting the electrode, and are arranged at a prescribed interval so that the convex curved surfaces face each other And a biosensor electrode unit, wherein the sample liquid is introduced onto the electrode by surface tension acting between them.   2. The biosensor according to claim 1, wherein the convex curved surface of the convex curved plate member is a cylindrical, columnar, crescent column, semi-column, elliptical column, fan column, or side column convex portion having at least one curved surface. Electrode part.   Simultaneous measurement of multiple items is possible by arranging the convex curved plates of a plurality of conductive curved plates made of a plurality of conductors facing each other around a cylinder or column made of a conductor at a specified interval. The biosensor electrode part according to claim 1.   The biosensor electrode part according to claim 1 or 2, wherein each convex curved member is joined to a support and the distance between the electrodes is defined by fixing the support to a spacer.   The biosensor electrode part according to claim 4, wherein the spacer is made of an electrically insulating material, and the support joined to the conductor is made of a conductive material, whereby the support constitutes a terminal connected to the measuring device. .   The biosensor electrode part according to claim 1 or 2, further comprising a stopper for defining a sample volume.   The biosensor electrode part according to claim 6, wherein the spacer serves as a stopper for defining the sample volume.   The biosensor electrode section according to any one of claims 1 to 7, wherein the sample volume is 1 µL or less. The biosensor according to any one of claims 1 to 8, wherein the movement of the sample liquid onto the electrode is performed by applying an electric potential to at least one member of the conductors constituting the electrode and utilizing electrowetting. Electrode part.   The biosensor electrode part according to any one of claims 1 to 9, which is used for a biosensor array in which a plurality of biosensor electrode parts are arranged.   The needle-integrated biosensor electrode section according to any one of claims 1 to 10, which is used together with a puncture needle.

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