JP4632178B2 - Operating method of anaerobic ammonia oxidation tank - Google Patents

Description

  The present invention relates to an operation method of an anaerobic ammonia oxidation tank, and more particularly to an operation method of an anaerobic ammonia oxidation tank that simultaneously denitrifies ammonia and nitrous acid in water to be treated by anaerobic ammonia oxidizing bacteria.

  Nitrogen components contained in sewage and industrial wastewater need to be removed for reasons such as causing eutrophication of lakes and marshes and reducing dissolved oxygen in rivers. Nitrogen components contained in sewage and industrial wastewater are mainly nitrogen components such as ammonia nitrogen, nitrite nitrogen, nitrate nitrogen, and organic nitrogen.

  Conventionally, this type of wastewater, if the nitrogen concentration is low, is also removed by ion exchange method and oxidation by chlorine, ozone, but in the case of medium to high concentration, biological treatment is adopted, Generally, it is operated under the following conditions.

  Biological treatment involves aerobic nitrification and anaerobic denitrification, and in aerobic nitrification, ammonia oxidizing bacteria (Nitrosomonas, Nitrosococcus, Nitrosospira, Nitrosolobus, etc.) and nitrite oxidizing bacteria (Nitrobactor, Nitrospina, Nitrococcus, Nitrospira, etc.) oxidize ammonia nitrogen and nitrite nitrogen, while anaerobic denitrification involves denitrification by heterotrophic bacteria (Pseudomonas denitrificans, etc.).

A nitrification tank for performing aerobic nitrification is operated within a load range of 0.2 to 0.3 kg-N / m ³ / day, and an anaerobic denitrification denitrification tank is loaded with a load of 0.2 to 0.4 kg-N / m. It is operated in the range of ³ / day. In order to treat the total nitrogen concentration of sewage of 30 to 40 mg / L, a residence time of 6 to 8 hours was required in the nitrification tank and 5 to 8 hours were required in the denitrification tank, and a large-scale treatment tank was required. In industrial wastewater containing only inorganic substances, nitrification tanks and denitrification tanks are designed with the same load as before, but organic substances are required for denitrification, and methanol with a concentration of 3 to 4 times the nitrogen concentration was added. . For this reason, there is a problem that not only the initial cost but also a great running cost is required.

  On the other hand, recently, a nitrogen removal method by an anaerobic ammonia oxidation method has attracted attention (for example, Patent Document 1). This anaerobic ammonia oxidation method is a method in which ammonia is used as a hydrogen donor, nitrous acid is used as a hydrogen acceptor, and ammonia and nitrous acid are simultaneously denitrified by an anaerobic ammonia oxidizing bacterium according to the following reaction formula.

(Chemical formula 1)
1.0 NH ₄ + 1.32NO ₂ + 0.066HCO ₃ + 0.13H ⁺ → 1.02N ₂ + 0.26NO ₃ + 0.066CH ₂ O _0.5 N _0.15 + 2.03H ₂ O
According to this method, since ammonia is used as a hydrogen donor, there are merits such as drastically reducing the amount of methanol used for denitrification and reducing the amount of sludge generated. It is considered to be an effective method.

However, anaerobic ammonia oxidizing bacteria that perform anaerobic ammonia oxidation reaction are a group of bacteria represented by Planctomycete, and it has been reported that the growth rate is as extremely low as 0.001 h ⁻¹ (Strous, M. et al .: Nature, 400, 446 (1999) Further, in Patent Document 2, the specific growth rate is a very small value of about 0.02 to 0.05 day- ^{1 and in} order to obtain twice the amount of cells. There is a report that it takes 14-35 days for culture.
JP 2001-37467 A JP 2003-24990 A

  For this reason, when trying to start up an anaerobic ammonia oxidation tank using anaerobic ammonia oxidizing bacteria from activated sludge, it takes a long time of acclimatization that has never been experienced before, and it is extremely inefficient There is.

  If it is a small laboratory-level experimental device, it is possible to shorten the start-up period by introducing sludge in which anaerobic ammonia-oxidizing bacteria have been cultured in advance into the anaerobic ammonia-oxidizing tank. If it is going to do the same thing with an actual apparatus, the culture plant which culture | cultivates anaerobic ammonia oxidation bacteria will be needed. However, in the practical application, the culture plant becomes huge, and not only a large equipment cost and operation management cost are required for operation, but also there is a disadvantage that it is necessary to adjust a large amount of nitrogen drainage for culturing. .

  Thus, in order to actually operate the anaerobic ammonia oxidation tank using the anaerobic ammonia oxidation method, there are still problems to be solved, and there is no actual operation example in Japan.

  The present invention has been made in view of such circumstances, the acclimatization period of anaerobic ammonia oxidizing bacteria having a slow growth rate can be shortened, it is not necessary to provide a culture plant, and anaerobic ammonia oxidizing bacteria have been extracted. It aims at providing the operating method of the anaerobic ammonia oxidation tank which does not also reduce the performance of one anaerobic ammonia oxidation tank.

According to claim 1 of the present invention, in order to achieve the above object, in anaerobic ammonia oxidation in which ammonia and nitrous acid are simultaneously denitrified by anaerobic ammonia oxidizing bacteria, acclimatization of the anaerobic ammonia oxidizing bacteria is performed. One part of the anaerobic ammonia-oxidizing bacteria is extracted from the anaerobic ammonia-oxidizing tank that is one actual device, and the other anaerobic ammonia-oxidizing bacterium that is used to acclimate the extracted anaerobic ammonia-oxidizing bacteria from now on a method of operating an anaerobic ammonium oxidation vessel to carry out the up operation up to put in, a fixing material which can freely flow through the anaerobic ammonium oxidizing tank, the anaerobic ammonium oxidation to the immobilizing material the immobilized material formed by gelling material or as the immobilizing material is deposited fixed bacteria and the anaerobic ammonium oxidizing bacteria The mixture polymerized using the anaerobic ammonium oxidizing bacteria is a comprehensive fixed fluidized immobilized materials in within gelling material comprising, in anaerobic ammonium oxidation vessel of the one real device, the immobilizing microorganisms 25% of the total amount of microbial immobilization material acclimatized, and the amount of microbial immobilization material withdrawn at one time from the anaerobic ammonia oxidation tank of one real device where the microbial immobilization material was acclimatized Withdrawing as follows and putting it into the anaerobic ammonia oxidation tank of the other actual device, and when the microorganism immobilization material is extracted from the anaerobic ammonia oxidation tank of the one actual device, A step of replenishing an anaerobic ammonia oxidizing bacteria tank with an unfamiliar new microorganism fixing material, and the microorganism fixing material from the anaerobic ammonia oxidizing tank of the one actual device Withdrawal is characterized in that the total amount of the acclimatized microbe-immobilized materials are withdrawn in a plurality of times so replaced in the new microbe-immobilized materials over a period of one month.
According to a third aspect of the present invention, in order to achieve the above object, in anaerobic ammonia oxidation in which ammonia and nitrous acid are simultaneously denitrified by anaerobic ammonia oxidizing bacteria, One part of the anaerobic ammonia oxidizing bacterium is withdrawn from the anaerobic ammonia oxidizing tank which is one of the actual devices that have been acclimatized, and the other anaerobic ammonia that is to be used to acclimatize the extracted anaerobic ammonia oxidizing bacteria from now on An operation method of an anaerobic ammonia oxidation tank that is put into an oxidation tank and started up, wherein the anaerobic ammonia oxidation bacteria are attached, fixed, or covered to an immobilizing material that can freely flow in the anaerobic ammonia oxidation tank. Using the immobilized fluid type microorganism immobilization material, the microorganism immobilization material is placed in the anaerobic ammonia oxidation tank of the one actual apparatus. The amount of microbial immobilization material extracted at one time from the anaerobic ammonia oxidation tank of one actual device to which the microbial immobilization material has been conditioned is reduced to 25% or less of the total amount of conditioned microbial immobilization material. Withdrawing so that the microbial immobilization material is extracted from the anaerobic ammonia oxidation tank of the one real device, and the anaerobic property of the one real device Replenishing the ammonia oxidizing bacteria tank with an unfamiliar new microorganism immobilizing material, and liquefying the acclimatized microorganism immobilizing material extracted from the anaerobic ammonia oxidizing tank of the one actual device, The entrapped immobilization support is formed by mixing activated sludge with the liquefied microorganism immobilization material and polymerizing the mixture of the mixed microorganism and the gelling material. Characterized in that it introduced into the anaerobic ammonium oxidation vessel of the other real devices.

    The present invention also serves to culture anaerobic ammonia-oxidizing bacteria in an anaerobic ammonia-oxidizing tank operated as an actual apparatus, and pulls out the cultured anaerobic ammonia-oxidizing bacteria and serves as a starter in another anaerobic ammonia-oxidizing tank On the basis of the idea, it is configured to shorten the acclimatization period of the other anaerobic ammonia oxidation tank and prevent the performance of one of the extracted anaerobic ammonia oxidation tanks, The anaerobic ammonia-oxidizing bacteria that have been acclimatized in the one anaerobic ammonia-oxidizing tank are designed to be microorganism-immobilized materials that are fixedly attached to the immobilizing material or fixed inclusively in order to facilitate the extraction. is there.

  According to the first aspect of the present invention, the anaerobic ammonia oxidizing bacteria that have been acclimatized in one anaerobic ammonia oxidizing tank are used for acclimatization of the other anaerobic ammonia oxidizing bacteria. The acclimatization period of ammonia-oxidizing bacteria can be shortened, and there is no need to provide a culture plant. In addition, by treating anaerobic ammonia-oxidizing bacteria as a microorganism immobilization material immobilized on an immobilizing material, the anaerobic ammonia-oxidizing bacteria can be extracted from one anaerobic ammonia-oxidizing tank or transferred to the other anaerobic ammonia-oxidizing tank. The anaerobic ammonia-oxidizing bacteria can be easily input, and the amount of extraction and the amount of input can be accurately controlled.

  In the present invention, the acclimatization period is a period required until the ammonia nitrogen concentration and the nitrite nitrogen concentration are simultaneously reduced to half, that is, the anaerobic ammonia oxidizing bacteria predominately propagate and exert anaerobic ammonia oxidizing activity. The period until it is done. In addition, the startup operation includes a case where the anaerobic ammonia oxidizing bacterium is started up in addition to the case where the newly installed anaerobic ammonia oxidizing tank is started up. In addition, one anaerobic ammonia oxidation tank and the other anaerobic ammonia oxidation tank may be provided in the same wastewater treatment facility, or may be provided in other wastewater treatment facilities, respectively.

  In the present invention, the amount of the microorganism-immobilized material extracted from the one anaerobic ammonia oxidation tank at a time is 25% or less of the total amount of the conditioned microorganism-immobilized material.

  This is because if the amount of the microorganism-immobilizing material extracted at one time from one of the acclimatized anaerobic ammonia oxidation tanks is 25% or less, the denitrification performance of one of the extracted anaerobic ammonia oxidation tanks will be improved. This is because there is almost no adverse effect. It is even better if the amount of the microorganism-immobilizing material extracted at a time is 10% or less with respect to the total amount.

  In this case, it is preferable to acclimatize an excessive microorganism fixing material in the one anaerobic ammonia oxidation tank on the assumption that the microorganism fixing material is pulled out.

  In this way, if one anaerobic ammonia oxidation tank is acclimatized with excess microbial immobilization material on the assumption that the microbial immobilization material is withdrawn, and an excessive amount of microbial immobilization material is extracted, anaerobic The required amount of microorganism-immobilizing material can always be held in the basic ammonia oxidation tank, and the denitrification performance is not affected at all. That is, the excess amount used an anaerobic ammonia oxidation tank as a culture tank.

In the present invention, the conditioned immobilization material is extracted from the one anaerobic ammonia oxidation tank, and the unaccustomed new microbial immobilization material is replenished. Here, the unfamiliar carrier means one having low anaerobic ammonia oxidation activity, and one having anaerobic ammonia oxidation activity of 1 (kg-N / m ³ -carrier / day) or less at a denitrification rate per carrier. Yes, it does not necessarily mean new. Unless denitration rate is “per carrier”, denitrification rate and volumetric load are evaluated by “per reactor volume”, and the unit is (kg-N / m ³ / day). The same applies hereinafter.

  If the extraction amount is 25% or less of the total amount of the microbial immobilization material, the denitrification performance of one of the extracted anaerobic ammonia oxidation tanks is hardly affected, but it is 25% in the next extraction. Since it exceeds, it can be pulled out only once. However, the amount of the microorganism fixing material in the anaerobic ammonia oxidation tank is not reduced by pulling out the microorganism fixing material and replenishing the unfamiliar microorganism fixing material as in the present invention. Therefore, if this is repeated, the acclimatized microorganism immobilization material can be successively drawn out from one anaerobic ammonia oxidation tank to another anaerobic ammonia oxidation tank to become an acclimatized inoculum.

Further, in the first aspect of the present invention, withdrawal of the microbe-immobilized materials from the one anaerobic ammonium oxidation tank, the total amount of the acclimatized microbe-immobilized materials are over 1 month new microbe-immobilized materials I pulled it out in multiple times so that it could be replaced .

  If a microorganism immobilization material is frequently extracted from an anaerobic ammonia oxidation tank many times in a short period of time, the proportion of the microorganism immobilization material that has not been fully adapted to the extracted anaerobic ammonia oxidation tank will increase. Denitrification performance is deteriorated. Therefore, if the extraction frequency is adjusted so that the microbial immobilization material has been withdrawn in several batches so that it is replaced with a new microbial immobilization material that has been replenished over one month, preferably over two months, it will be replenished. Since the new microbial immobilization material has been adapted, the influence on denitrification performance is reduced. As a pulling method, it is preferable to pull evenly. That is, it is preferable to make the amount of drawing multiple times the same every time, and if the amount is different for each drawing, the denitrification performance tends to fluctuate.

  In the present invention, the conditioned microbial immobilization material extracted from the one anaerobic ammonia oxidation tank is liquefied, and activated sludge is mixed with the liquefied microbial immobilization material, and the mixed microorganism and the gelled material are mixed. It is preferable to form a entrapping immobilization support by polymerizing a mixture of the above and the like, and to put the entrapping immobilization support into the other anaerobic ammonia oxidation tank.

  In this way, the number of entrapping immobilization carriers can be increased by mixing activated sludge with the liquefied entrapping immobilization carrier and increasing the amount. This is because the predominance ratio of the anaerobic ammonia-oxidizing bacteria is high, and it is easy to be adapted.

  As described above, according to the operation method of the anaerobic ammonia oxidation tank of the present invention, the anaerobic ammonia oxidation bacteria already adapted in one anaerobic ammonia oxidation tank is used for acclimatization of the other anaerobic ammonia oxidation bacteria. Therefore, it is possible to shorten the acclimatization period of anaerobic ammonia-oxidizing bacteria with a slow growth rate, eliminating the need for a culture plant, and reducing the performance of one anaerobic ammonia-oxidizing tank from which anaerobic ammonia-oxidizing bacteria have been extracted There is nothing.

  Hereinafter, preferred embodiments of the method for operating an anaerobic ammonia oxidation tank according to the present invention will be described in detail with reference to the accompanying drawings.

  FIG. 1 is a conceptual diagram of an anaerobic ammonia oxidation apparatus that implements the method of operating an anaerobic ammonia oxidation tank of the present invention.

  Of the two vertical anaerobic ammonia oxidation tanks shown in FIG. 1, one anaerobic ammonia oxidation tank is anaerobic ammonia oxidation having an acclimatized microorganism immobilization material 14A predominately propagated by anaerobic ammonia oxidizing bacteria. The other anaerobic ammonia oxidation tank is an unaccustomed anaerobic ammonia having a microorganism immobilization material 14B in which anaerobic ammonia oxidizing bacteria have not yet prevailed. In the oxidation tank (hereinafter referred to as “unfamiliar tank 12”), the start-up is performed from now on. In addition, although microorganisms fixing material 14A, 14B of this Embodiment demonstrates in the example of the fixed bed type microorganism fixing material 14A, 14B which adheres microorganisms around a nonwoven fabric, it is not limited to this. . For example, an adhesion / flow type microorganism immobilization material that adheres and immobilizes microorganisms around a granular immobilization material that can flow in a tank such as a gel or resin beads, or a microorganism containing and immobilizing microorganisms inside a hydrous gel It is possible to use a microbial immobilization material of inclusion / flow type that can freely flow inside.

  Examples of the material for the immobilization material include polyvinyl alcohol, alginic acid, polyethylene glycol gel, and plastic materials such as cellulose, polyester, polypropylene, and vinyl chloride, but are not particularly limited. As for the shape, it is preferable to use a product that has been formed into a spherical shape, a cylindrical shape, a porous shape, a cubic shape, a sponge shape, a honeycomb shape, or the like. Granules utilizing microbial self-granulation can also be used in the present invention.

  In the tanks of the acclimatized tank 10 and the unfamiliar tank 12, microbial immobilization materials 14A and 14B using a rod-shaped nonwoven fabric as an immobilization material are vertically immersed in the water to be treated, and are passed through the raw water pipes 16A and 16B. Water to be treated supplied from the bottoms of the tanks 10 and 12 by the raw water pumps 18A and 18B flows as an upward flow in the tanks 10 and 12, and is discharged from the upper parts of the tanks 10 and 12 through the treated water pipes 20A and 20B. Here, the microorganism immobilization material 14A shown in black in FIG. 1 means that the microorganism immobilization material 14B shown in white means an acclimatized microorganism immobilization material in which anaerobic ammonia-oxidizing bacteria predominately propagated. Means an unfamiliar microbial immobilization material or a newly supplemented microbial immobilization material in which anaerobic ammonia-oxidizing bacteria have not yet prevailed.

Dispersions 22A and 22B are provided at the bottoms of the conditioned tank 10 and the unfamiliar tank 12, and the water to be treated flowing from the raw water pipes 16A and 16B flows upward in the tanks 10 and 12 evenly. At the same time, the concentration of nitrite nitrogen (NO ₂ -N) is locally increased to prevent the anaerobic ammonia oxidizing bacteria from being deactivated.

  As shown in FIGS. 2 and 3, the conditioned tank 10 and the unconditioned tank 12 may be provided in the same wastewater treatment plant or in separate wastewater treatment plants.

  FIG. 2 shows that, in the same wastewater treatment plant, the acclimatization of the anaerobic ammonia-oxidizing bacteria from the A-series acclimatized tank 10 to the B-series unacclimatized tank 12 to be started up from now on is performed. It shows that the already-immobilized microorganism immobilization material 14A is moved. In this way, when operating a large number of treatment series A, B, C, D in the same wastewater treatment plant, the anaerobic ammonia oxidizing bacteria of the treatment series that has been launched earlier are combined with seed sludge of other treatment series. can do.

  FIG. 3 shows that the conditioned microorganism immobilization material 14A is moved from the already treated (operating) wastewater treatment plant A of anaerobic ammonia-oxidizing bacteria to another wastewater treatment plant F to be started up. Is shown. Thus, between the waste water treatment plants A and F, the sludge of the waste water treatment plant A that has been started up can be transferred to one waste water treatment plant F that is about to start operation.

  In order to carry out the operation method of the anaerobic ammonia oxidation tank of the present invention using the acclimatized tank 10 and the non-accommodated tank 12, as shown in FIG. A part of the chemical material 14 </ b> A is pulled out and put into the unfamiliar tank 12. Then, the acclimatized tank 10 is supplemented with an unaccustomed microorganism immobilization material 14B, and the operation is continued. On the other hand, in addition to the microorganism immobilization material 14A from the acclimatized tank 10, a start-up operation is performed on the unaccustomed tank 12. In this case, the acclimatized microorganism immobilization material 14A is not necessarily used as it is, and may be added to the unacclimated tank 12 as an acclimatized sludge by peeling off the immobilized anaerobic ammonia-oxidizing bacteria.

  In such an operation method of the present invention, the microorganism immobilization material 14A acclimatized in the conditioned tank 10 is the entrapping immobilization carrier, and a part of the total amount of the entrapping immobilization carrier in the acclimated tank 10 is charged into the unacclimated tank 12. In this case, the number of entrapping immobilization carriers to be introduced into the unfamiliar tank 12 may be increased by the method for producing entrapping immobilization carriers shown in FIG.

  As shown in FIG. 4, the entrapping immobilization carrier extracted from the conditioned tank 10 is liquefied by a homogenizer or the like (liquefaction step). At this time, it is preferable to add hydrazine, which is an intermediate metabolite of anaerobic ammonia-oxidizing bacteria, and a reducing agent that prevents contamination of oxygen, and / or hydroxylamine, which is an intermediate metabolite of anaerobic ammonia-oxidizing bacteria. Next, water or activated sludge is mixed with the liquefied entrapping immobilization support (mixing step). Next, the mixture mixed in the mixing step and the gelling material as the entrapping immobilization material are mixed, and water or dilute sulfuric acid as a reaction modifier is added to the gelling material. Then, by adding a polymerization initiator such as potassium peroxide to cause a polymerization reaction, the gelling material is gelled and the mixture is comprehensively fixed to the gelling material (inclusive fixing step). In this way, the number of entrapping immobilization carriers can be increased by mixing activated sludge with the liquefied entrapping immobilization carrier and increasing the amount. The predominance ratio of anaerobic ammonia-oxidizing bacteria is high, and it is in a state of being easily adapted.

  In addition, when the unfamiliar microorganism immobilization material 14A to be replenished to the acclimatized tank 10 and the unfamiliar microorganism immobilization material 14B to be charged to the unfamiliar tank 12 are of the adhesion fixing type, the immobilization material before being charged. Alternatively, the microorganisms such as sludge may be preliminarily adhered to the container. At this time, the organic matter adhering to the sludge inhibits the acclimatization of the anaerobic ammonia oxidizing bacteria. Therefore, after reducing the BOD concentration in the tank to 50 mg / L or less, the acclimatized anaerobic ammonia oxidation is performed. It is preferable to introduce bacteria. Alternatively, an immobilizing material to which microorganisms are not attached may be introduced to attach microorganisms during operation.

  Table 1 shows test results explaining that the acclimatization period of anaerobic ammonia-oxidizing bacteria having a slow growth rate can be shortened by performing the operation method of the present invention.

  In the test, an unfamiliar tank 12 configured as an experimental device was made up using an anaerobic ammonia oxidation method using inorganic synthetic wastewater. The addition in Table 1 is the case where the conditioned sludge itself floating in the conditioned tank 10 is added to the unconditioned tank 12 in addition to the addition of the conditioned microorganism immobilization material 14A. When not added.

  Test 1 is a case where a nonwoven fabric to which activated sludge to which anaerobic ammonia-oxidizing bacteria are not acclimatized is attached is put into the unfamiliar tank 12 and is started up.

As a start-up operation condition, a new nonwoven fabric was immersed in sewage sludge (sludge having a sludge concentration: MLSS 30000 mg / L), and the nonwoven fabric adhered with sewage sludge was put into the unfamiliar tank 12. Moreover, the sewage sludge itself was also added to the unfamiliar tank 12 except the nonwoven fabric to which the sewage sludge was adhered. For the raw water composition, referring to AAvan de Graaf et.al Microbiology (1996), 142, p2187-2196, the composition shown in Table 2 was used, and the concentration of nitrite nitrogen (NO ₂ -N) and ammoniacity were used. The operation was performed by changing the nitrogen (NH ₄ -N) concentration.

(Remarks) Ellement S1: EDTA: 5g / L, FeSO ₄ : 5g / L
T.A. Ellement S2: EDTA: 15g / L, ZnSO ₄ · 7H ₂ O: 0.43g / L, CoCl ₂ · 6H ₂ O: 0.24g / L, MnCl ₂ · 4H ₂ O: 0.99g / L, CuSO ₄ · 5H ₂ O : 0.25g / L, NaMoO ₄・ 2H ₂ O: 0.22g / L, NiCl ₂・ 6H ₂ O: 0.19g / L, NaSeO ₄・ 10H ₂ O: 0.21g / L, H ₃ BO ₄ : 0.014g / L
As a result, in the unfamiliar tank 12, simultaneous denitrification of ammonia and nitrous acid was started about 124 days after the start of operation, and anaerobic ammonia oxidation activity was confirmed. Thereafter, a denitrification rate of 0.45 (kg-N / m ³ / day) was confirmed on the 220th day. In the case of Test 1, the acclimatization period is 220 days, but the denitrification rate using the anaerobic ammonia oxidation method is still not sufficient.

  Test 2 is a case where nitrified sludge adhered to a non-woven fabric is put into an unfamiliar tank 12 and started up. Nitrification sludge is sludge sampled from the nitrification tank of the nitrification / denitrification equipment. As operating conditions, first, a new nonwoven fabric was dipped in nitrified sludge (sludge having a sludge concentration: MLSS 30000 mg / L), and the nitrified sludge was adhered to the nonwoven fabric. In addition to the non-woven fabric to which nitrified sludge adhered, nitrified sludge itself was also added to the unfamiliar tank 12. The addition amount is the same as that in Test 1 and is the same as the addition amount when the following test is also added. Others are the same as those in Test 1.

  As a result, even after 180 days of operation, simultaneous denitrification of ammonia and nitrous acid was not started, and anaerobic ammonia oxidation activity could not be confirmed. That is, the anaerobic ammonia-oxidizing bacteria did not proliferate preferentially by acclimatization, and the unacclimated tank 12 could not be started up.

  From Test 1 and Test 2, an unfamiliar microbial immobilization material in which anaerobic ammonia-oxidizing bacteria do not predominately propagate cannot take up the unfamiliar tank 12, or it takes a very long time to start up. You can see that

Tests 3 to 7 are operation methods of the present invention. The acclimatized microbial immobilization material 14A is extracted from the acclimatized tank 10 that has been operated in advance, and is put into the unacclimated tank 12 to start up the unacclimatized tank 12. It is what I did. In other words, the acclimatized tank 10 that had been operated in advance was charged with 80% as an apparent filling rate into the tank using a non-woven fabric as a fixed bed. Then, as shown in Table 2, nitrite nitrogen concentration (NO ₂ -N) using inorganic synthetic wastewater adjusted 200 mg / L, ammonia nitrogen concentration (NH ₄ -N) in 250 mg / L, Operation was performed at a denitrification rate of 3 to 5 (kg-N / m ³ / day). In this way, as the microorganism immobilization material 14 </ b> A of the conditioned tank 10 in tests 3 to 7, a seed bed having the above-mentioned nonwoven fabric as a fixed bed is used, and this is added to the unfamiliar tank 14. The amount of acclimated sludge was tested to be almost constant. In the test, the form of the microorganism immobilization material 14A in the conditioned tank 10 is a non-woven fabric fixed bed, but is not limited to this. Moreover, the form of the microorganism immobilization material added to the unfamiliar tank 14 is not limited to the non-woven fabric. In the test, in addition to the non-woven fabric, various forms of inclusion immobilization support, PVA gel beads, and granules were performed.

In Test 3, 5% of the conditioned microorganism immobilization material 14A is extracted from the conditioned tank 10 and put into the unconditioned tank 12, and the remaining 95% is a new article to which unconditioned activated sludge is attached. Of the microorganism immobilization material 14B. Further, the conditioned sludge itself in the conditioned tank 10 was not added. As a result, the denitrification rate of the unconditioned tank 12 becomes 3.0 (kg-N / m ³ / day) 52 days after the start of operation, and the anaerobic ammonia oxidation activity at the same level as that of the adapted tank 10 is obtained. I was able to get it. That is, the acclimatization period of Test 3 was 52 days, and a sufficient denitrification rate using the anaerobic ammonia oxidation method could be obtained.

In Test 4, 4% of the conditioned microorganism-immobilizing material 14A extracted 5% from the conditioned tank 10 is put into the unconditioned tank 12, and the remaining 1% is stripped of sludge to remove the unconditioned tank 12. It was thrown into. As a result, after 40 days from the start of operation, the denitrification rate of the unconditioned tank 12 became 3.3 (kg-N / m ³ / day), and the conditioned sludge itself was added, resulting in a better result than in Test 3. It was. The acclimatization period of Test 4 was 40 days, and a sufficient denitrification rate using the anaerobic ammonia oxidation method could be obtained.

  In Test 5, sludge was peeled off from the conditioned microorganism-immobilizing material 14A extracted 5% from the conditioned tank 10, the mixture mixed with the activated sludge was fixed with a polyethylene glycol-based gel, and formed into a pellet. A comprehensive immobilization support was obtained. The gel concentration was 15% by weight and the mixture concentration in the carrier was 1.5% by weight.

As a result of carrying out the test by introducing this entrapped immobilization carrier into the unfamiliar tank 12 so that the carrier filling rate is 30%, the denitrification rate of the unfamiliar tank 12 is 3 in about one month (31 days) after the start of operation. It became 3 (kg-N / m ³ / day), and it became possible to acclimatize in a shorter time than test 4.

In Test 6, the sludge was peeled off from the conditioned immobilization material 14A extracted 5% from the conditioned tank 10, and the gel beads made of PVA (polyvinyl alcohol) were immersed in the sludge for 24 hours, and then the sludge and the PVA gel beads were added. It put into the unfamiliar tank 12. The filling rate of the PVA gel beads into the unfamiliar tank 12 was set to 50% as an apparent volume. As a result, after 60 days from the start of operation, the denitrification rate of the unfamiliar tank 12 is increased to 1.8 (kg-N / m ³ / day), although it is smaller than the other tests 3, 4, and 5 above. I was able to. The reason why the denitrification rate was lower than those in Tests 3, 4, and 5 is considered to be that part of the acclimatized sludge adhering to the PVA gel beads peeled off and flowed out of the tank.

In Test 7, the sludge is peeled off from the conditioned immobilization material 14A extracted 5% from the conditioned tank 10, and the granules are immersed in the sludge for 24 hours, and then the unconditioned tank 12 is filled with the sludge and granules. did. The unfilled tank 12 was filled with granules so that the apparent volume was 40%, and was operated using a UASB apparatus. As a result, 50 days after the start of operation, the denitrification rate of the unconditioned tank 12 becomes 3.5 (kg-N / m ³ / day), and anaerobic ammonia oxidation activity at the same level as that of the conditioned tank 10 is obtained. I was able to. That is, the acclimatization period in Test 7 was 50 days, and a sufficient denitrification rate using the anaerobic ammonia oxidation method could be obtained.

  Moreover, as can be seen from Tests 3 to 7 in Table 1, the acclimatization period when the operation method of the present invention is performed is 1 to 2 months. From this, the unfamiliar microorganism immobilization material 14B replenished to the acclimatized tank 10 needs a acclimatization period of 1 month or more, and preferably acclimatizes for 2 months or more. Therefore, the extraction frequency from the conditioned tank 10 is such that the total amount of the conditioned microbial immobilization material 14A in the conditioned tank 10 is replenished to the microbial immobilization material 14B supplemented over one month or more, more preferably two months or more. It is preferable to pull out little by little at the switching speed. If the extraction is performed faster than the extraction frequency, a large amount of unfamiliar microorganism-fixing material occupies the acclimatized tank 10 and the denitrification performance of the acclimatized tank 10 is deteriorated.

  In the operation method of the present invention, the amount of the microorganism-immobilizing material 14 </ b> A extracted from the conditioned tank 10 at a time is preferably 25% or less of the total amount of the microorganism-immobilizing material 14 </ b> A of the conditioned tank 10.

  FIG. 5 is a test result of investigating the relationship between the amount of the microorganism immobilization material 14A withdrawn from the conditioned tank 10 at a time and the denitrification performance in the conditioned tank 10, and the denitrification performance measures the denitrification rate. It was evaluated by doing.

The test was conducted using the inorganic synthetic wastewater in Table 2 adjusted to nitrite nitrogen concentration (NO ₂ -N) 220 mg / L and ammoniacal nitrogen concentration (NH ₄ -N) 200 mg / L, and operating conditions with HRT being 3 hours. 6 conditioned tanks 10 (experimental equipment) were prepared. Nonwoven fabric was used as an immobilization material for the microorganism immobilization material 14A. In all six tanks, the denitrification rate before extracting the microorganism-immobilizing material 14A was 2.5 (kg-N / m ³ / day).

  And the extraction amount of the microorganism immobilization material 14A from the six acclimatized tanks 10 is 3%, 5%, 10%, 15%, 25%, 30% with respect to the whole microorganism immobilization material 14A. Pulled out.

The result is shown in FIG. The horizontal axis in FIG. 5 is the number of days that have elapsed since the microorganism-immobilized material 14 </ b> A is pulled out, and the vertical axis is the denitrification rate of the conditioned tank 10. As can be seen from FIG. 5, when the withdrawal amount is 10% or less, there is almost no decrease in the denitrification rate with the passage of the number of days after withdrawal.
When the withdrawal amounts are 15% and 25%, the denitrification rate slightly decreases from immediately after withdrawal until about 10 days, and returns to the original denitrification rate after 10 days. However, when the withdrawal amount reaches 30%, the denitrification rate decreases abruptly as the number of days since withdrawal has elapsed, and the denitrification rate is about 1 (kg-N / m ³ / day) on the fifth day. ), And after 30 days, there was no sign of recovery of the denitrification rate.

  From the results of FIG. 5, the amount of the conditioned microorganism immobilization material 14A that is extracted from the acclimatized tank 10 at a time is preferably 25% or less of the total amount of the microorganism immobilization material 14A, more preferably 10% or less. Yes, particularly preferably 5% or less.

  Therefore, when the acclimatized tank 10 is also used as a culture tank for culturing anaerobic ammonia-oxidizing bacteria, the amount of the microorganism-immobilizing material 14A extracted from the acclimatized tank 10 is 25% or less, and 1-2. It is important to pull out so as to switch to the microorganism-immobilized material 14B that has been replenished over a month or more.

In the operation method of the present invention, it is preferable to acclimatize the excess microorganism immobilization material 14A in the acclimatized tank 10 on the assumption that the microorganism immobilization material 14A is extracted. Using the acclimatized tank 10 operating at a denitrification rate of 3.0 (kg-N / m ³ / day), an excessive amount of 5% microbial immobilization material 14A corresponding to the extraction amount is excessive before extraction. The 5% microbial immobilization material 14A was withdrawn at a time, and at the same time, a continuous operation was performed by performing an extraction / replenishment operation to replenish a new microbial immobilization material 14B once a month. As a result, even when 5% of the microorganism-immobilizing material 14A was pulled out from the conditioned tank 10, the denitrification rate of the conditioned tank 10 did not decrease, and the quality of the treated water from the conditioned tank 10 did not deteriorate. Thereby, the acclimatized tank 10 was also used as a culture tank, and the acclimatized microorganism immobilization material 14A could be obtained once a month and used as an inoculum for other unaccustomed tanks 12.

  The above is a description of the extraction amount and extraction frequency of the microorganism immobilization material 14A extracted from the acclimatized tank 10 without deteriorating the denitrification performance, but the same amount of the microorganism immobilization material 14A from the acclimatized tank 10 is described. Since the denitrification performance of the conditioned tank 10 is adversely affected if the portion in the conditioned tank 10 to be extracted is different, it is necessary to set the extraction site appropriately.

  FIG. 6 shows the denitrification rate distribution of the conditioned tank 10 in order to set an appropriate extraction site.

In the test, the vertical conditioned tank 10 in which the water to be treated flows in the upward flow shown in FIG. 1 is used, and the microbial immobilization material 14A using a non-woven fabric as the immobilization material is used to perform inorganic synthesis. A wastewater treatment test using wastewater was conducted. In the acclimatized tank 10, there is provided a microorganism fixing material 14A in which a plurality of round bar-shaped nonwoven fabrics having a diameter of 100 mm and a length of 300 mm are immersed and arranged in the vertical direction. The composition of the water to be treated was the inorganic synthetic waste water shown in Table 2, and the operation was performed by changing the nitrite nitrogen (NO ₂ -N) concentration and the ammonia nitrogen (NH ₄ -N) concentration. The water temperature was 36 ° C, the HRT was 6 hours, and the nitrogen load was about 1.8 to 2.2 (kg-N / m ³ / day). The denitrification rate at this time was 1.3 to 1.62 (kg-N / m ³ / day).

  Then, one month later, one of the microorganism immobilization materials 14A was taken out from the conditioned tank 10, cut into 6 strips by 5 cm along the length direction, and the denitrification rate for each strip was examined. The results are shown in FIG.

  The bar graph in FIG. 6 shows the denitrification rate of each of the six strips obtained by cutting the nonwoven fabric of the microorganism-immobilizing material 14A. Reference numeral 1 denotes a strip at the bottom of the nonwoven fabric, which is a strip cut at a position of 50 mm from the bottom of the nonwoven fabric. Similarly, no. 2 is the second strip from the bottom. 3 is the third strip from the bottom. 4 is the fourth strip from the bottom. 5 is the fifth strip from the bottom. 6 is the sixth strip from the bottom.

  As can be seen from the results in FIG. 6, No. 2 corresponding to the lower half of the microorganism-immobilized material 14A. 1-No. No. 3 strip shows a high denitrification rate, No. 3 corresponding to the upper half of the microorganism immobilization material 14A. 4-No. Six strips showed a low denitrification rate. This is because the denitrification rate in the conditioned tank 10 is not uniform when viewed in the example of the vertical conditioned tank 10, and the inflow side (bottom part) to the outflow side (top part) of the treated water. It can be seen that the denitrification rate on the inflow side is large and the denitrification rate on the outflow side is small when viewed from the direction of the water to be treated. In other words, the activity of the anaerobic ammonia oxidizing bacteria on the inflow side is high, and the activity of the anaerobic ammonia oxidizing bacteria on the outflow side is small. This means that there is a risk of adversely affecting the denitrification performance of the conditioned tank 10 when the microbial immobilization material 14A is pulled out from a portion in a direction perpendicular to the flow direction of the water to be treated flowing in the conditioned tank 10. . That is, when the extraction site in the direction orthogonal to the flow direction of the water to be treated is the inflow side, a large amount of highly active anaerobic ammonia-oxidizing bacteria is extracted, and the denitrification rate of the conditioned tank 10 is increased. Will be significantly worse.

  Therefore, in the operation method of the present invention, when extracting the microorganism immobilization material 14A from the conditioned tank 10 so as not to adversely affect the denitrification performance, the flow direction of the water to be treated flowing in the conditioned tank 10 is It is important to extract the microorganism-immobilizing material 14A evenly from the parts in the parallel direction. In place of the microorganism fixing material 14A using a nonwoven fabric as the fixing material, a plurality of fixed bed type microorganism fixing materials 14A filled with cylindrical containers with PVA gel beads are immersed in the conditioned tank 10 in the vertical direction. In the same manner, the pull-out test was performed, and in this case, the microorganism-immobilized material 14A was evenly pulled out from the portion in the direction parallel to the flow direction of the water to be treated flowing in the conditioned tank 10, The denitrification rate of the conditioned tank 10 was hardly adversely affected. Thus, appropriately setting the site where the microorganism immobilization material 14A is pulled out is not limited to the fixed bed type microorganism immobilization material such as PVA gel beads filled in a nonwoven fabric or a cylindrical container.

Therefore, in order to confirm the relationship between the extraction site of the microorganism fixing material 14A and the denitrification performance, as shown in the conceptual diagram of FIG. The conditioned microorganism immobilization material 14A was pulled out from both the parts in the direction orthogonal to the direction. That is, 10 microorganism immobilization materials 14A are vertically arranged in the acclimatized tank 10, and each microorganism immobilization material 14A is cut into 10 strips, and each is supported by a support member (not shown). Two tanks were prepared. In the actual acclimatized tank 10, the microorganism immobilization material 14A is also arranged in the front and back direction of FIG. 7, but here, for easy understanding of the explanation, the microorganism immobilization material 14A arranged on one plane shown in the drawing is used. explain. Accordingly, an assembly of ten strips 13 surrounded by a broken line in FIG. 7, that is, a part in a direction parallel to the flow direction of the water to be treated if the microorganism immobilization material 14A before being cut into the strips 13 is pulled out. The microorganism immobilization material 14A is pulled out from the above. Further, when the aggregate 15 of the ten strips 13 surrounded by the two-dot chain line is pulled out, the microorganism immobilization material 14A is pulled out from a portion in a direction orthogonal to the flow direction of the water to be treated. In any case, the extraction amount is the same. About these two acclimatized tanks 10, the inorganic synthetic waste water shown in Table 2 was used, and the continuous operation was performed with a nitrogen load of about 1.8 to 2.2 (kg-N / m ³ / day). . The denitrification rate at this time was 1.3 to 1.62 (kg-N / m ³ / day). And after confirming that the denitrification performance was stabilized because the concentration of nitrite nitrogen in the treated water discharged from the conditioned tank 10 became 5 mg / L, the nonwoven fabric 1 was added from one conditioned tank 10. Ten strips 15 which were orthogonal to the flow direction were pulled out from the main drawing and the other conditioned tank 10. Ten strip assemblies 15 were drawn from the strip assembly 15 on the inflow position located 50 to 75 mm below the nonwoven fabric.

  As a result, when one non-woven fabric was pulled out from a portion parallel to the flow direction of the water to be treated, the nitrite nitrogen concentration of the treated water temporarily increased to 30 mg / L, but about 10 days. The nitrite nitrogen concentration in the treated water decreased to the original 5 mg / L. On the other hand, when ten strip assemblies 15 are pulled out from the portion orthogonal to the flow direction of the water to be treated, the denitrification rate of the conditioned tank 10 is reduced, and the nitrite nitrogen concentration of the treated water is 100 mg / The denitrification performance did not recover even after 30 days, exceeding L When the anaerobic ammonia oxidizing bacteria in the acclimatized tank 10 were examined, they were inactivated.

  In this way, by pulling out the microorganism fixing material 14A evenly from the portion in the direction parallel to the flow direction of the water to be treated flowing in the conditioned tank 10, the denitrification performance of the conditioned tank 10 is almost adversely affected. The microorganism immobilization material 14A can be pulled out without giving.

  FIG. 8 shows an immobilization material so that the microorganism immobilization material 14A can be easily pulled out from a portion in a direction parallel to the flow direction of the water to be treated flowing in the vertical conditioned tank 10. A certain nonwoven fabric is unitized. 8A is a conceptual side view, and FIG. 8B is a conceptual top view.

  As shown in FIG. 8, in the water to be treated in the vertical acclimatized tank 10, a large number of microbial immobilization materials 14A using a rod-shaped nonwoven fabric as an immobilization material are vertically arranged in two upper and lower stages. The microorganism immobilization materials 14A are arranged with a certain gap (interval) therebetween. The upper and lower two-stage microorganism immobilization material 14A is arranged such that the upper microorganism immobilization material 14A is disposed above the lower gap, and the lower microorganism immobilization material 14A is disposed below the upper gap. . Moreover, the upper end and lower end of the nonwoven fabric in the microorganism immobilization material 14 </ b> A in the same row are connected by a connecting rod 17. As a result, as shown in FIG. 8B, a square plate-like unit 21 is formed in which a plurality of microorganism immobilization materials 14A are supported at intervals between a pair of upper and lower connecting rods 17 and 17. On each of the upper and lower stages of the conditioned tank, a plurality of units 21 are supported by a frame casing 23 that is open on all sides. In the casing 23, guide grooves 25 for inserting the units 21 are formed in a vertical direction at regular intervals. By inserting the units 21 into the guide grooves 25, the plurality of units 21 are arranged in the flow direction of the water to be treated. They can be arranged parallel to each other.

  In this way, if the microorganism immobilization material 14A is made into a fixed bed type and immersed in the conditioned tank 10 as a plurality of units 21 parallel to the flow direction of the water to be treated, the unit 21 is pulled out to be treated. The microorganism-immobilizing material 14A can be pulled out evenly and easily from a portion in a direction parallel to the water flow direction. In addition, the arrangement of the upper and lower microbial immobilization materials 14A in the acclimatized tank 10 is staggered, and the baffle plate 22A for dispersion is arranged at the inlet of the water to be treated. It can be dispersed throughout the conditioned tank 10. This prevents an area where the nitrite nitrogen concentration locally increases in the conditioned tank 10. In the anaerobic ammonia oxidation method that simultaneously denitrifies ammonia and nitrous acid, nitrous acid is necessary. However, if the concentration of nitrite nitrogen exceeds 80 mg / L, the activity of anaerobic ammonia oxidizing bacteria is inhibited. This is because it is not preferable that an area in which the concentration of nitrite nitrogen is locally increased is generated in the finished tank 10.

  FIG. 8 shows an example of the vertical conditioned tank 10, but FIG. 9 shows the case of the horizontal conditioned tank 10, and the same members and devices in FIG.

  In the case of the horizontal conditioned tank 10, the water to be treated (raw water) flows from the inflow part (left side in FIG. 9) to the outflow part (right side in FIG. 9) as shown in FIG. 9 (A). ) In the horizontal direction. Therefore, as shown in FIG. 9B, among a large number of conditioned microorganism fixing materials 14A arranged at intervals in the conditioned tank 10, a row of microorganisms parallel to the flow direction of the water to be treated. Only the fixing material (non-woven fabric shown in black) is connected by the connecting rod 17 in the same manner as in FIG. 6 to form a unit 21, and this unit 21 is pulled out. In this way, by forming one extraction unit 21, it is not necessary to make all the microorganism-immobilizing material 14 </ b> A into the unit 21, so that the unitization becomes easy and the production cost of the unit 21 is low. Become. Further, as shown in FIG. 9C, the units 21 provided in a row are divided into two units 21A and 21B on the inflow side and the outflow side, and the units 21A and 21B are extracted according to the denitrification performance of the conditioned tank 10. May be changed. However, in the case of the horizontal conditioned tank 10 as well, as with the vertical conditioned tank 10 of FIG. 8, all of the microorganism immobilization material 14A may be unitized.

Conceptual diagram of an anaerobic ammonia oxidation apparatus that is implemented by the method for operating an anaerobic ammonia oxidation tank of the present invention. The schematic diagram which showed the case where the operation method of the anaerobic ammonia oxidation tank of this invention is performed in the same wastewater treatment plant The schematic diagram which showed the case where the operation method of the anaerobic ammonia oxidation tank of this invention is performed in a different wastewater treatment plant Explanatory drawing of the method of manufacturing the entrapping immobilization carrier to be put into the unfamiliar tank when the microbial immobilization material accustomed in the acclimatized tank is the entrapping immobilization carrier Relationship diagram showing the relationship between the amount of microbial immobilization material withdrawn from a conditioned tank at once and the denitrification rate Explanatory drawing explaining distribution of denitrification speed in acclimatized tank Explanatory drawing explaining the extraction site | part of the microorganisms immobilization material for not deteriorating the denitrification performance of the acclimatized tank In the anaerobic ammonia oxidation apparatus for carrying out the present invention, a vertical acclimatized tank comprising a unit in which a microorganism immobilization material is unitized so as to be parallel to the flow direction of water to be treated. The anaerobic ammonia oxidation apparatus which implements this invention WHEREIN: The block diagram of the horizontal type | formula already-fitted tank provided with the unit which unitized the microorganisms fixing material so that it might become parallel to the flow direction of to-be-processed water.

Explanation of symbols

  DESCRIPTION OF SYMBOLS 10 ... Acclimatized tank, 12 ... Unaccommodated tank, 13 ... Strip, 14A ... Acclimatized microorganism immobilization material, 14B ... Unaccommodated or supplemented microorganism immobilization material, 15 ... Strip assembly, 16A, 16B ... Raw water piping , 17 ... Connecting rod, 18A, 18B ... Raw water pump, 20A, 20B ... treated water pipe, 21 ... unit, 23 ... casing, 25 ... guide groove

Claims (5)

In anaerobic ammonia oxidation in which ammonia and nitrous acid in treatment water are simultaneously denitrified by anaerobic ammonia-oxidizing bacteria, from the anaerobic ammonia-oxidizing tank, which is one actual device that has been adapted to the anaerobic ammonia-oxidizing bacteria, An anaerobic ammonia oxidation tank that pulls out a part of the anaerobic ammonia oxidation bacteria and puts the extracted anaerobic ammonia oxidation bacteria into the anaerobic ammonia oxidation tank, which is the other actual equipment to be acclimatized. Driving method,
An immobilization material capable of freely flowing in the anaerobic ammonia oxidation tank, wherein the anaerobic ammonia oxidation bacteria are adhered and immobilized on the immobilization material, or an immobilization material formed of a gelling material as the immobilization material. Using a fluid type microorganism immobilization material in which a mixture containing a material and the anaerobic ammonia oxidizing bacteria is polymerized and the anaerobic ammonia oxidizing bacteria are comprehensively immobilized in a gelled material ,
Acclimatizing the microorganism immobilization material in the anaerobic ammonia oxidation tank of the one real device;
The microbial immobilization material is pulled out from the anaerobic ammonia oxidation tank of one actual apparatus to which the microbial immobilization material has been conditioned, and the microbial immobilization material is withdrawn so that the amount of the microbial immobilization material is 25% or less of the total amount of the conditioned microbial immobilization material. The step of putting it in the anaerobic ammonia oxidation tank of the actual device of
When the microorganism-immobilized material is pulled out from the anaerobic ammonia oxidation tank of the one actual device, a step of replenishing an unfamiliar new microorganism-immobilized material to the anaerobic ammonia-oxidizing bacteria tank of the one actual device; With
The extraction of the microorganism-immobilizing material from the anaerobic ammonia oxidation tank of the one actual device is divided into a plurality of times so that the total amount of the conditioned microorganism-immobilizing material is replaced with the new microorganism-immobilizing material over one month or more. The method of operating an anaerobic ammonia oxidation tank characterized by being pulled out.   2. The method of operating an anaerobic ammonia oxidation tank according to claim 1, wherein an excess amount of the microorganism immobilization material is acclimatized to the anaerobic ammonia oxidation tank on the premise that the microorganism immobilization material is pulled out. In anaerobic ammonia oxidation in which ammonia and nitrous acid in treatment water are simultaneously denitrified by anaerobic ammonia-oxidizing bacteria, from the anaerobic ammonia-oxidizing tank, which is one actual device that has been adapted to the anaerobic ammonia-oxidizing bacteria, An anaerobic ammonia oxidation tank that pulls out a part of the anaerobic ammonia oxidation bacteria and puts the extracted anaerobic ammonia oxidation bacteria into the anaerobic ammonia oxidation tank, which is the other actual equipment to be acclimatized. Driving method,
Using a fluid-type microorganism immobilization material in which the anaerobic ammonia-oxidizing bacteria are attached and fixed or included and fixed to an immobilization material that can freely flow in the anaerobic ammonia oxidation tank,
Acclimatizing the microorganism immobilization material in the anaerobic ammonia oxidation tank of the one real device;
The microbial immobilization material is pulled out from the anaerobic ammonia oxidation tank of one actual apparatus to which the microbial immobilization material has been conditioned, and the microbial immobilization material is withdrawn so that the amount of the microbial immobilization material is 25% or less of the total amount of the conditioned microbial immobilization material. The step of putting it in the anaerobic ammonia oxidation tank of the actual device of
When the microorganism-immobilized material is pulled out from the anaerobic ammonia oxidation tank of the one actual device, a step of replenishing an unfamiliar new microorganism-immobilized material to the anaerobic ammonia-oxidizing bacteria tank of the one actual device; With
The acclimatized microorganism immobilization material extracted from the anaerobic ammonia oxidation tank of the one actual apparatus was liquefied, activated sludge was mixed with the liquefied microorganism immobilization material, and the mixed microorganism and the gelled material were mixed. A method for operating an anaerobic ammonia oxidation tank, comprising forming a entrapping immobilization support by polymerizing a mixture, and introducing the entrapping immobilization support into an anaerobic ammonia oxidation tank of the other actual apparatus.   4. The method of operating an anaerobic ammonia oxidation tank according to claim 3, wherein an excess amount of the microorganism immobilization material is acclimatized to the anaerobic ammonia oxidation tank on the premise that the microorganism immobilization material is pulled out.   The extraction of the microorganism-immobilizing material from the anaerobic ammonia oxidation tank of the one actual device is divided into a plurality of times so that the total amount of the acclimatized microorganism-immobilizing material is replaced with the new microorganism-immobilizing material over one month. The method of operating an anaerobic ammonia oxidation tank according to claim 3 or 4, wherein the anaerobic ammonia oxidation tank is pulled out.

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