JP4616097B2 - Method for producing nutritional functional materials derived from genus fish - Google Patents

Description

The present invention relates to a method for producing a nutritional functional material derived from a genus fish, and more particularly to a method for producing a nutritional functional material derived from a genus fish for antioxidant, antihypertensive and anti-aging purposes. is there.

Nutritional functional materials that are being actively researched have the effect of preventing various diseases, so it is possible to expect a reduction in medical costs, which is an enormous burden, and also to support the possibility of daily health. have. In particular, there are many reports that marine raw materials that have been eaten in the past have had effective functionalities through research, and further search for nutritional functional materials is still desired.

There are also several problems with using marine raw materials such as fish. For example, certain components such as collagen are abundant in tissues such as skin and tendons, but in large animals such as cattle and pigs, it is easy to separate and remove skin from other tissues and organs. But it is difficult for fish.

Therefore, as a method for extracting specific components such as collagen from fish without separating such tissues, a method for producing non-bovine-derived modified collagen as disclosed in JP-A-2004-99659 has been developed. However, this method only uses the whole fish body including bones, muscles and internal organs as raw materials, and does not commercialize the whole fish body as a nutritional functional material. The effect has not been elucidated. In addition, the manufacturing process is still complicated.

The present inventor has paid attention to the genus fish as a raw fish species. Although this fish species has a considerable catch as an outer road in the vicinity of Toyama Bay, some of it is used locally for food and is not shipped to the secondary market. However, as a result of confirming and researching its functionality, it was found that it contains an excellent nutritional functional component useful for antioxidant action, antihypertensive action, anti-aging action, and learning enhancement.

Therefore, the present inventor makes effective use of “genge” whose use is limited as described above, and further develops a nutritional functional material using the whole fish body including bones, muscles and internal organs of the genus fish. The purpose is to provide a relatively simple method for producing a nutritional functional material derived from astragalus fish that does not require the separation of fish tissues as described above and does not inhibit various nutritional functions of the astragalus fish. Successfully developed.

According to the present invention, there is provided a method for producing a nutrient functional material from a genus fish, a step of washing and selecting fish of the genus fish, cutting all or part of the selected fish into an appropriate size, and forming a minced shape. A step of heat-treating a minced sample in water, a step of freeze-drying the heat-treated sample, and a step of pulverizing the freeze-dried sample. By these steps, a freeze-dried powder material derived from genus fish having nutritional functionality is obtained.

Freeze-drying allows the sample to be dehydrated without heat treatment, thereby preventing thermal denaturation of the contained components. As a result, the nutritional functional material obtained by the present invention can contain a nutritional functional component derived from a genus fish that is useful for antioxidant, antihypertensive, anti-aging, and learning enhancement at a high content.

There are six typical species of genus fish: Yokosuji Kurogenge, Sarasagaji, Koguchibigenge, Kantengage, Shirogenge and Ashinga Genge. It is. These fish live on continental shelf slopes with a depth of 200 m or more, and some are used for food, but most are discarded even if caught. All the genus fish can be used in the present invention, but the common algae known as “Genge” is easily available.

In the present invention, the entire fish body including bones, muscles, and internal organs can be used as a raw material. Furthermore, by using only a specific part of the fish tissue as a raw material, a nutritional functional material with an increased specific functionality can be obtained. It can also be manufactured.

For example, when the surface tissue is peeled off from the fish body by washing and sorting and only the surface tissue of the genus fish rich in collagen is produced according to the production method according to the present invention, the collagen mainly used in cosmetics, health foods, etc. The nutritional functional composition material.

In addition, by adding coenzyme Q10, γ-tocophenol, DHA (docosahexaenoic acid), edible fatty oil, glycerin fatty acid ester, and the like, the antioxidant action, antihypertensive action, antiaging action and learning enhancement function are synergistically It can also be used as a nutritional functional material derived from an enhanced genus fish.

The nutritive functional material derived from the Astragalus fish according to the present invention thus produced can be simply blended into a general food or the like in its original form, and can also be encapsulated, tableted, tableted, or capletted. By doing so, it is also possible to provide a shape suitable for regular carrying.

In the case of a shape suitable for regular carrying such as tableting, conventional excipients, disintegrants and the like can be further added, and encapsulation is not limited to soft capsules, It may be a hard capsule.

The method for producing a nutritional functional material according to the present invention can be effectively utilized without losing the nutritional function (component) derived from the Astragalus fish. Oxidation, collagen content and peptide functionality, antioxidant activity of coenzyme Q10, cell activation and anti-aging, antioxidant activity of γ-tocophenol and improvement of water balance in the body, and further DHA (docosahexaenoic acid ) Provides a method for producing a nutritional functional material that can promote metabolic activation comprehensively by reducing and anti-oxidizing cholesterol and triglycerides, and that can be expected to prevent aging, enhance beauty and enhance physical fitness. it can.

Example 1
After washing and sorting the Kantengage captured in Toyama Bay, it was cut into a size of about 2 cm, and 1 kg of the cut lump was transferred to a mixer equipped with a rotary blade to obtain a sample crushed into minced shapes. The minced sample was boiled in water boiled to about 100 ° C. and then freeze-dried to obtain a nutritional functional material derived from a genus genus fish that had been dried to be agglomerated. The pulverized composition is pulverized into a powder form, and then an excipient, a disintegrant and the like are added and mixed, and then tableted by a known method to give 50 mg of the tablet composed of the nutritional functional material according to the present invention. Produced.

Example 2
100 mg of the nutritional function according to the present invention obtained by adding excipients, disintegrants and the like to the same powdery nutritional functional material obtained in Example 1 and mixing them, and tableting by the same method as in Example 1 A tablet made of a functional material was prepared.

Example 3
Ingredients shown in Table 1 below are added to and mixed with the same pulverulent functional nutrient material obtained in Example 1, and the mixed material is filled into capsules by a known method, and the internal volume Soft capsules containing 300 mg of the nutritional functional material according to the present invention were made.

Example 4
Ingredients shown in Table 2 below are added to and mixed with the same pulverulent functional functional material obtained in Example 1, and the mixed material is filled into capsules by a known method. Hard capsules containing 330 mg of the nutritional functional material according to the present invention were made.

Test Example The active oxygen scavenging activity was measured using an electron spin resonance apparatus using soft capsules and hard capsules containing tablet-type Gengefreeze Dry and Gengefreeze Dry of Examples 1 to 4 as samples. JEOL, JES-FR30 type: electron spin resonance apparatus (ESR) manufactured by JEOL Ltd. was used as a measuring instrument. Samples used in this test were tablets made of Genge Freeze Dry (Genge FD) of Examples 1 and 2: 50 mg and 100 mg each, 1 soft capsule containing Genge Freeze Dry of Example 3 The test was performed using one and two hard capsules containing one and two hard capsules containing the Genge Freeze Dry of Example 4. As pre-treatment, 0.1 NHCL (corresponding to pH in human stomach, pH 1.5), distilled water of pH 8.0 (corresponding to pH in human small intestine), MeOH, and 70% EtOH Each of the antioxidant active substances in the sample injection was extracted using the above solvent. Using the obtained supernatant as a sample, in a 96-well microwell, 0.2 mM FeSO ₄ solution: 37.5 μL, H ₂ O: 30 μL, sample: 30 μL, 10-fold diluted solution DMPO (5,5-Dimethyl-1 -Pyrroline-N-Oxide, Dojindo Laboratories): 20 μL, 1 mM H ₂ O ₂ : 75 μL were sequentially added and mixed to obtain a reaction solution. The reaction solution was allowed to react at room temperature for 45 seconds, and then ESR analysis was performed to measure the rate of elimination of hydroxyl radicals. The test results are shown in Table 3 below.

From the test results shown in Table 3, active oxygen scavenging activity was observed in tablets made of Genge FD according to the present invention and capsules containing Genge FD. In the soft capsule of Example 3, no active oxygen scavenging activity was observed, but it is considered that the fat used in the encapsulation process inhibited the active oxygen scavenging activity.
In other samples, high active oxygen scavenging activity was shown by extraction with MeOH or weak alkaline water.

Claims (1)

A method for producing a nutritional functional material from genus fish,
(A) a process of washing and sorting fish bodies;
(B) a step of cutting the entire selected fish body including bones, muscles and / or internal organs into an appropriate size to form a minced sample;
(C) heat-treating the minced sample in water;
(D) freeze-drying the heat-treated sample,
(E) crushing the freeze-dried sample;
Said method comprising.