JP4587281B2 - 癌転移抑制能測定する方法およびその測定器 - Google Patents
癌転移抑制能測定する方法およびその測定器 Download PDFInfo
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- JP4587281B2 JP4587281B2 JP2004182162A JP2004182162A JP4587281B2 JP 4587281 B2 JP4587281 B2 JP 4587281B2 JP 2004182162 A JP2004182162 A JP 2004182162A JP 2004182162 A JP2004182162 A JP 2004182162A JP 4587281 B2 JP4587281 B2 JP 4587281B2
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Description
がん転移研究会編『がんの浸潤・転移研究マニアル』 金芳堂 1994年
さらに、特許文献1に記載された方法は、癌細胞の観点から癌細胞の内皮細胞への浸潤能、運動能を評価する系である。
1) 癌細胞の原発巣での増殖
2) 周辺組織への浸潤、
3) 血管内への侵入
4) 血管内移動
5) 転移臓器血管への定着
6) 血管外脱出を経て、
転移臓器組織で増殖し、転移巣形成に至るステップにおいて、血管内への侵入に相当し、病期は進行した段階であり、転移抑制、転移予防が手遅れとなステージとなる。
International Journal of Oncology 21:541-546 2002
2 ケモタキシスチャンバー
3 24穴培養プレート
4 細胞外マトリックス構成成分
5 血管内皮細胞
6 癌細胞
7 培養コンパートメント上層部
8 培養コンパートメント下層部
9 血管内皮細胞の開裂程度部分
10 抵抗測定コンパートメント容器
11 コンパートメント上層部電極
12 コンパートメント下層部電極
13 抵抗測定容器蓋
14 抵抗測定容器EVOM(WPI社)
15 抵抗測定コンパートメントEndohm(WP1社)
Claims (2)
- 抗転移剤の候補となる物質を、癌細胞の側ではなく、癌細胞が転移する,宿主側の内皮細胞の観点から選別するための癌転移抑制能測定する方法であって、血管内皮細胞と癌細胞を非接触共存培養することで生ずる、血管内皮細胞の細胞間隔程度を、内皮細胞を横切る電気的抵抗量で評価することを特徴とする癌転移抑制能測定する方法。
- 上部が開口し、底面に癌細胞を配置する24穴細胞培養プレートと、化学走性、即ち,ケモタキシスを解析するための癌転移抑制能測定器であって、抵抗測定コンパートメント容器を設け、該容器の内部に、底部に細孔を有するポリカーボネイトフィルターを設けたケモタキシスチャンバーと、前記24穴細胞培養プレートの底面が、ケモタキシスチャンバーと接触しないよう懸隔手段により懸隔し、小区画を形成し、該小区画内の血管内皮細胞を飽和状に培養し、前記24穴細胞培養プレート内に癌細胞を共存培養することで生ずる血管内皮細胞の細胞間隔程度を、前記抵抗測定コンパートメント容器の上層部と下層部との間に、前記ポリカーボネイトフィルターを介して一定最短距離で電極を挿み、電気的抵抗量で評価することを特徴とすることを特徴とする癌転移抑制能測定器。
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JP2004182162A JP4587281B2 (ja) | 2004-06-21 | 2004-06-21 | 癌転移抑制能測定する方法およびその測定器 |
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JP2004182162A JP4587281B2 (ja) | 2004-06-21 | 2004-06-21 | 癌転移抑制能測定する方法およびその測定器 |
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JP2006003293A JP2006003293A (ja) | 2006-01-05 |
JP4587281B2 true JP4587281B2 (ja) | 2010-11-24 |
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Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
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JP5330657B2 (ja) * | 2007-07-24 | 2013-10-30 | 泰 田川 | 癌転移能評価方法及び癌転移能評価のための三次元二重膜構造 |
DE102009052673A1 (de) * | 2009-11-12 | 2011-05-19 | Nanoanalytics Gmbh | Vorrichtung zur Bestimmung der Impedanz von Zellschichten |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05211893A (ja) * | 1992-01-31 | 1993-08-24 | Agency Of Ind Science & Technol | 血管内皮モデル調製用器及びこれを用いた癌細胞の転移 能の測定方法 |
JPH06316543A (ja) * | 1993-03-10 | 1994-11-15 | Morinaga Milk Ind Co Ltd | スチルベン誘導体とスチルベン同族体誘導体及びそれ らの用途 |
JP2002518678A (ja) * | 1998-06-12 | 2002-06-25 | セネス リミティド | 高処理量スクリーン |
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EP1539992B1 (en) * | 2002-07-20 | 2009-11-11 | Acea Biosciences, Inc. | Impedance based devices and methods for use in assays |
FR2846426B1 (fr) * | 2002-10-28 | 2004-12-10 | Bio Merieux | Procede de dosage du ngf pour le diagnostic in vitro du cancer du sein et utilisation en therapie |
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05211893A (ja) * | 1992-01-31 | 1993-08-24 | Agency Of Ind Science & Technol | 血管内皮モデル調製用器及びこれを用いた癌細胞の転移 能の測定方法 |
JPH06316543A (ja) * | 1993-03-10 | 1994-11-15 | Morinaga Milk Ind Co Ltd | スチルベン誘導体とスチルベン同族体誘導体及びそれ らの用途 |
JP2002518678A (ja) * | 1998-06-12 | 2002-06-25 | セネス リミティド | 高処理量スクリーン |
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