JP4571540B2 - Method for treating seaweed containing fucoidan - Google Patents

Description

  The present invention is a treatment of seaweed containing fucoidan for extracting an extract such as fucoidan contained in edible drought seaweed Mozuku, Mekabu, Wakame, Kombu, Root Kombu, Hijiki, Asao, Funori, Red Moku, etc. Regarding the method.

  Conventionally, seaweeds such as mozuku, especially fucoidan contained in Okinawa mozuku, have excellent anti-cholesterol action, blood clearing action, anti-blood coagulation action, anti-cancer action, anti-AIDS virus action, anti-gastric ulcer action, etc. It is known to have a pharmacological effect, and therefore, development for efficiently extracting such fucoidan has been performed.

For example, a method has been disclosed in which fucoidan is extracted from cultured Okinawa Mozuku using an inorganic acid (concentration: 0.2 N) such as hydrochloric acid or sulfuric acid as an extractant and taking about 24 hours at room temperature ( Patent Document 1).
JP 10-165114 A

  However, the above-described fucoidan extraction method uses a highly corrosive strong acid as an extractant, and thus has a disadvantage of easily corroding manufacturing equipment.

  Therefore, by using an inorganic material having high corrosion resistance, it is possible to prevent the corrosion of the manufacturing equipment to some extent. However, since the inorganic material having high corrosion resistance is extremely expensive, it is economically disadvantageous. is there.

  Moreover, in the above extraction method, it is difficult to set the average molecular weight of the obtained fucoidan within a desired molecular weight, for example, in the range of 10,000 to 5,000,000.

Therefore, in the present invention, the salt from the seawater adhering to the seaweed is desalted, and the foreign matter and small animals attached to the seaweed are removed and washed, By immersing seaweed that has been desalted and washed in the desalting / washing process in an aqueous solution to which bittern has been added, the viscosity of alginic acid contained in seaweed is reduced by minerals such as Mg ions contained in bittern. Immersion treatment step to suppress, dehydration treatment step to dehydrate the seaweed soaked in the immersion treatment step, and shredding to shred the seaweed dehydrated in the dehydration treatment step using a shredding device A softening treatment step of swelling and softening the shredded seaweed by pressurizing and heating or immersing the shredded seaweed shredded in the shredding treatment step in the treatment step; In the softening process By circulating the softened shredded seaweed in the extraction processor, the shredded seaweed is continuously pulverized and refined, and the extract containing fucoidan is extracted and processed, The extract extracted in the extraction processing step is subjected to a low molecular weight treatment step through a low molecular weight treatment device having the same configuration as the extraction treatment device, and the low molecular weight treatment treatment is performed in the low molecular weight treatment step. The present invention provides a method for treating a seaweed containing fucoidan, characterized by comprising adding a bittern to a very fine insoluble fibrous polysaccharide and performing a final adjustment of a bittern .

  Here, bittern includes bittern (natural pure bittern) produced from natural sea salt (unadjusted salt) and bittern obtained by an ion exchange membrane. Natural pure bittern is made from 100% seawater. It is extracted from natural salt called natural sea salt produced by the Hiragana method and contains nearly 80 elements such as magnesium, calcium, potassium, copper, silicon, zinc, lithium and strontium. It is what.

  The present invention is also characterized by the following configuration.

(1) The extraction processor includes a flow path forming casing and a plate-shaped first support piece having an outer diameter close to the inner peripheral surface of the flow path forming casing and having a communication hole in the center. A first chamber forming body in which a large number of cylindrical first chamber forming pieces are arranged in an aligned state on one side surface, and a space that is spaced apart from the inner peripheral surface of the flow path forming casing by a certain width. A second chamber forming body formed by arranging a plurality of cylindrical second chamber forming pieces in an aligned state on one side surface of a plate-like second support piece formed in an outer diameter, The first and second chamber forming bodies of the first set and the first and second chamber forming bodies of the second set are stacked in an interview state in the path forming casing, and each forming body has the same size. In addition, the first support piece and the second support piece formed in the same shape are arranged so as to face each other three first chamber forming pieces so that one second chamber forming piece extends evenly. The three first chamber forming pieces and the one second chamber forming piece are communicated, and the peripheral portions of the first and second sets of second support pieces to be laminated and the inner peripheral surface of the flow path forming casing In between, a ring-shaped moving flow path is formed, and the shredded seaweed is provided on the first chamber forming body and the second chamber forming body in the first set from the communication hole. The first chamber forming piece → the second chamber forming piece → the first chamber forming piece → the second chamber forming piece, while flowing in a separated direction substantially perpendicular to the flow-down direction while meandering, reaches the outer peripheral edge. The first chamber forming piece → the second chamber forming piece → the first chamber forming piece on the peripheral side provided in the first chamber forming body and the second chamber forming body of the second set through the moving flow path → Extraction containing fucoidan from shredded seaweed that is repeatedly merged by flowing in the approach direction substantially perpendicular to the flow direction while meandering like the second chamber forming piece There is characterized in that so as to be extraction process, processes by the extraction processor that.

(2) The extraction processor is provided with a narrow flow path forming groove part formed by continuously forming a concave groove extending in the axial direction on the inner peripheral wall of the first and second chamber forming pieces, In the narrow channel forming groove when the shredded seaweed meanders and flows in the first and second chamber forming pieces, the area in contact with the shredded seaweed is increased. And processing by the extraction processor .

(1) The seaweed treatment method containing fucoidan according to the present invention described in claim 1 includes a desalting / cleaning treatment step, a dipping treatment step, a dehydration treatment step, a shredding treatment step, a softening treatment step, and an extraction treatment step. And a low molecular weight treatment process.

  In this way, the seaweed containing fucoidan is pretreated by immersing it in an aqueous solution to which bittern has been added, so the mineral alginate contained in the bittern suppresses the viscosity of alginic acid contained in the seaweed. can do.

  As a result, the seaweed as a subsequent process can be refined to easily extract an extract containing fucoidan, and the extraction efficiency of the extract can be increased.

  Then, by reducing the size of seaweed, fucoidan having a low molecular weight can be efficiently extracted.

  At this time, since a highly corrosive strong acid is not used as the extractant, it is possible to prevent a problem that the manufacturing equipment is corroded.

(2) The seaweed treatment method containing fucoidan according to the present invention described in claim 2 is the center provided by the shredded seaweed on the first chamber formation body and the second chamber formation body from the communication hole. The first chamber forming piece on the part side → the second chamber forming piece → the first chamber forming piece → the second chamber forming piece, while flowing in a separated direction substantially perpendicular to the flow-down direction while meandering, the outer peripheral edge The first chamber forming piece on the peripheral side provided in the first chamber forming body and the second chamber forming body in the second set through the moving flow path, and the second chamber forming piece → first chamber The extract containing fucoidan is extracted from shredded seaweed by flowing in the approach direction substantially perpendicular to the flow direction while meandering from the formed piece to the second chamber formed piece and repeatedly joined together. It is made to process by the extraction processor characterized by this.

  In this way, seaweed can be refined at the time of diversion by repeating the diversion and merging in a continuous manner, such as diversion → merge → diversion → merge. It is possible to efficiently perform a continuous processing operation that can extract the contained extract in a low temperature environment.

  At this time, since the extract can be extracted by refining the seaweed, it is possible to physically shear the polymer alginic acid which becomes an obstacle to the extraction, thereby reducing the viscosity of the alginic acid. As a result, an extract containing fucoidan can be efficiently extracted in a short time.

  And since a fiber can be ground | pulverized to a submicron level, the development of the foodstuff containing a fiber is attained, and it becomes easy to eat and drink the fiber shunted uniformly.

  In addition, the extract can be extracted without using chemicals such as inorganic chemicals and organic acids, so that loss of extracted components due to the chemicals can be avoided, and corrosion of the extractor due to the chemicals can be avoided. Can do.

  Furthermore, even seaweeds such as red mok that are hard and difficult to process can be pulverized quickly, and the extraction efficiency of the extract can be ensured satisfactorily in all seaweeds.

  Moreover, since high extraction efficiency can be ensured even under low temperature conditions (for example, 10 ° C. to 30 ° C.), it is possible to eliminate the trouble of preparing the environment under high temperature conditions (for example, 60 ° C. to 110 ° C.). Thus, the work efficiency for the extraction process can be increased.

And the average molecular weight of the fucoidan in the obtained extract can be easily set within a desired low molecular weight, for example, in the range of 10,000 to 60,000, and the fineness of the fiber can be achieved. .

  As a result, fucoidan that has been reduced in molecular weight and dramatically improved in vivo absorption can be efficiently extracted.

  Moreover, such fucoidan has excellent pharmacological effects such as anticholesterol action, blood clearing action, anticoagulation action, anticancer action, anti-AIDS virus action, and antigastric ulcer action.

  Furthermore, since the dietary fiber that is diverted to the extract is micronized to the nano level, it can be diverted uniformly.

  Moreover, it can be easily combined with moromi vinegar or ume vinegar containing organic substances and organic acids because of pH stability (maintenance of quality) and ease of drinking.

(3) In the method for treating seaweed containing fucoidan according to the present invention as set forth in claim 3, the grooves extending in the axial direction are continuously formed in the inner peripheral walls of the first and second chamber forming pieces in the circumferential direction. A narrow channel forming groove is formed, and the shredded seaweed in the narrow channel forming groove when the shredded seaweed meanders and flows in the first and second chamber forming pieces. It is made to process by the extraction processor characterized by having made the area which touches to increase .

Thus, when shredded seaweed is shunted physically and forcibly when shunted through the narrow channel forming groove, and when joined through the narrow channel forming groove, The extract is further finely divided by being physically and forcibly shredded. In other words, the tip periphery of the narrow channel forming groove is used as an orifice, and hydrodynamic shearing when shredded seaweeds pass through, pulverization by impact destruction, and further mixing by mechanical cavitation, etc. It is done efficiently. Therefore, when seaweed containing fucoidan is flowed, fucoidan molecules contained in seaweed are physically cleaved, and low molecular weight fucoidan is easily and reliably extracted as an extract. The extraction efficiency of fucoidan can be increased.

  Embodiments of the present invention will be described below with reference to the drawings.

  That is, the seaweed treatment method containing fucoidan according to the present invention comprises a desalting / cleaning treatment step (a), an immersion treatment step (b), a dehydration treatment step (c), and shredding as shown in FIG. Treatment step (d), softening treatment step (e) (f), extraction treatment step (g), concentration step (h), filtration treatment step (i) and low molecular weight treatment step (j) bittern addition step (k ) Or ultrafiltration step (l) and bittern addition step (m).

  Below, each said process is demonstrated, referring FIG.1 and FIG.2.

(1) Desalination / cleaning process (a)
In the desalting / cleaning treatment step (a), seaweeds 42 are accommodated in the desalting / cleaning treatment tank 41, and water is continuously supplied from the water supply unit 43 to the desalting / cleaning treatment tank 41. By allowing only water to overflow from the desalting / washing tank 41, salt derived from seawater adhering to the seaweed 42 is desalted, and foreign substances and small animals adhering to the seaweed 42 are removed. This is the process of cleaning.

  At this time, the desalting / cleaning treatment tank 1 is subjected to the desalting / cleaning treatment for a certain period of time, for example, 20 minutes while stirring at low speed.

(2) Immersion treatment process (b)
The immersion treatment step (b) is a pretreatment process of seaweed containing fucoidan, and an aqueous solution 45 to which bittern N is added is accommodated in the immersion treatment container 44, and the seaweed 42 is immersed in the aqueous solution 45. It is a journey.

  Here, the concentration of the bittern in the aqueous solution 45 is 0.2% to 1.5%, preferably 0.5%.

  And the time to immerse is 10 minutes-60 minutes, Preferably, it is 30 minutes.

  The temperature is preferably room temperature.

  This immersion treatment step (b) suppresses the viscosity of alginic acid contained in the seaweed 42 by immersing the seaweed 42 in the aqueous solution 45 to which the bittern N is added, and minerals such as Mg ions contained in the bittern N. The purpose is to do.

(3) Dehydration process (c)
In the dehydration step (c), the desalted seaweed 42 is transferred into the rotating tank 46 of the centrifuge, and the seaweed 42 is dehydrated by rotating the rotating tank 46 for a certain time, for example, 10 minutes. It is the process to do.

  Further, the dehydration treatment of the seaweed 42 can be carried out by moving it to a sieve and leaving it naturally.

(4) Shredding process (d)
The shredding process (d) is a process of shredding the dehydrated seaweed 42 using a shredding device such as a mixer 47.

  Here, the seaweed 42 is shredded uniformly to about 1 mm to 5 mm. Reference numeral 8 denotes shredded seaweed 42, hereinafter referred to as shredded seaweed.

(5) Softening process (e) (f)
The softening treatment step (e) is a step of swelling and softening the shredded seaweed 48 by pressurizing and heating the shredded seaweed 48.

  Here, the pressure to be applied is, for example, 1.2 to 2.0 atm, preferably 1.6 atm. The heating temperature is, for example, 102 to 120 ° C., preferably 115 to Can be set.

  This softening process (e) is a process for efficiently extracting the extract from the shredded seaweed 48 in the subsequent extraction process (g).

  The softening treatment step (f) is a step of swelling the softened seaweed 48 by immersing the shredded seaweed 48 in hot water at 80 ° C. to 100 ° C.

  These softening treatment steps (e) and (f) can be selected according to preference by the operator, and the shredded seaweed 48 that has been softened through any of the steps (e) and (f) Then, for example, it is cooled to room temperature.

(6) Extraction process (g)
The extraction processing step (g) continuously pulverizes the chopped seaweed 48 by repeatedly circulating the softened chopped seaweed 48 in the extraction processor 1 provided in the extract extraction processing apparatus A. And extracting the extract 6 containing fucoidan.

Here, the extraction processor 1 has a return channel 10 interposed between the inflow channel connection port portion 27 and the outflow channel connection port portion 28 of the processor body 20, and the upstream side of the processor body 20. An upstream three-way valve 11 and a downstream three-way valve 12 are provided in a portion of the return channel 10 located on the downstream side. The detailed configuration of the extraction processor 1 will be described later.

The processor body 20 forms a flow path from upstream (upper in the present embodiment) to downstream (lower in the present embodiment), and joins a number of flow dividing chambers 8 in the flow path. The shredded seaweed 48 that flows down in the same flow path by alternately arranging the chambers 9 in the flow-down direction is repeatedly divided in the multiple flow-dividing chambers 8 and repeatedly in the multiple merge-chambers 9. The combined flow is configured to be refined while being sequentially repeated in the flow direction.

  That is, a certain amount of shredded seaweed 48 is supplied into the processor main body 20 via the upstream three-way valve 11 so that the shredded seaweed 48 can be refined in the processor main body 20. The shredded seaweed 48 is shunted, merged, shunted, merged, and so on, so that the seaweed can be refined at the time of shunting and fucoidan at the time of joining. The continuous processing operation that can extract the extract containing sucrose can be performed efficiently in a low temperature environment.

  At this time, the shredded seaweed 48 can be refined and the extract can be extracted. Therefore, the alginic acid that is an obstacle to extraction can be physically sheared to reduce the viscosity of the alginic acid. As a result, an extract containing fucoidan can be efficiently extracted in a short time.

  Moreover, the shredded seaweed 48 chopped in the processor main body 20 is discharged from the outlet channel connection port 28 → downstream three-way valve 12 → return channel 10 → upstream three-way valve 11 → processor main body 20 inflow. The flow path connection port 27 is returned to the processing unit main body 20 so that the circulation processing operation for refining can be performed again.

  In this manner, the average molecular weight of fucoidan in the obtained extract can be easily set within a desired low molecular weight range, and the fineness of the fiber can be achieved.

  Here, this circulation processing operation can be repeated, for example, 2 to 10 times, depending on the degree of refinement of the fiber.

(7) Concentration process (h)
The concentration step (h) is a step of evaporating the moisture of the extract 6 obtained in the above-described extraction treatment step (g) by heating the extract 6 to 50 ° C. to 80 ° C. under reduced pressure conditions.

  In this way, in the concentration step (h), the extremely fine insoluble fibrous polysaccharide contained in the refined extract 6 can be uniformly divided.

(8) Filtration process step (i)
The filtration process step (i) is a process of filtering the extract 6 extracted in the extraction process step (g) through the coarse filter 50.

  Here, as the coarse filter 50, a coarse filter of 120 mesh to 400 mesh can be used.

  In this way, some insoluble fibrous polysaccharides that have not been refined in the extraction treatment step (h) are removed.

  At this time, 0.2% to 1.0% of insoluble fibrous polysaccharide is removed with respect to 10 parts by weight of the extract 6, but the extract 6 extracted by the extraction processor 1 is uniform. Therefore, the passability of the coarse filter 50 is good.

(9) Low molecular weight treatment process (j)
In the molecular weight reduction process (j), the extract 6 filtered through the coarse filter 50 described above has the same configuration as the extraction processor 1 of the extract extraction processing apparatus A provided in the extraction process (g). This is a process of reducing the molecular weight through the low molecular weight processor 51.

  In this way, the extract 6 is subjected to low molecular weight treatment by repeatedly diverting the flow in the multiple diversion chambers 8 and repeatedly repeating the flow in the multiple confluence chambers 9 in the downstream direction. Therefore, the average molecular weight of fucoidan in the obtained extract 6 can be easily set to a desired low molecular weight, for example, in the range of 10,000 to 60,000, and the fineness of the fiber can be achieved. Can be achieved.

  As a result, fucoidan that has been reduced in molecular weight and dramatically improved in vivo absorption can be efficiently extracted.

  Moreover, such fucoidan has excellent pharmacological effects such as anticholesterol action, blood clearing action, anticoagulation action, anticancer action, anti-AIDS virus action, and antigastric ulcer action.

  Furthermore, since the dietary fiber that has been diverted to the extract 6 has been refined to the nano level, it can be evenly diverted.

  Moreover, it can be easily combined with moromi vinegar or ume vinegar containing organic substances and organic acids because of pH stability (maintenance of quality) and ease of drinking.

  In addition, according to the grade which makes the extract 6 low molecular weight, the circulation processing operation in this low molecular weight processing process can be performed twice to 5 times, for example.

  In addition, depending on the type of seaweed, the ratio of the extract 6 to water can be adjusted to 50% V / V to 100% V / V by adding water before entering the molecular weight reduction treatment step (j). . Alternatively, depending on the type of seaweed, the concentration step (h) can be omitted.

(10) Bittern addition process (k)
In the bittern addition step (k), final adjustment is performed by adding bittern N to the nano-level ultrafine insoluble fibrous polysaccharide that has been subjected to the low molecular weight reduction treatment step (j). It is a journey.

  In the bittern addition step (k), a final extract 52 in which nano-level extremely fine insoluble fibrous polysaccharide and bittern N are uniformly separated can be obtained.

  Here, the concentration of bittern N added to the insoluble fibrous polysaccharide can be set to 0.5% to 5%, preferably 2%.

  The final extract 52 contains a low-molecular fucoidan, can be used as a raw material for soft drinks, and is excellent in absorbability to the human body. Pharmacological effects such as anticoagulant action, anticancer action, anti-AIDS virus action, and anti-gastric ulcer action are also excellent.

  At this time, the fluidity of blood can be improved and the relaxation effect can be enhanced by the synergistic effect of the bittern containing mineral components and fucoidan.

(11) Ultrafiltration process (l)
The ultrafiltration step (l) is a step in which the ultrafiltration device 53 performs ultrafiltration treatment on the extract 6 that has been extracted in the low molecular weight reduction treatment step (j).

Here, as the ultrafiltration device 53, for example, a polyacrylonitrile film, a polyamide film, a ceramic film, or a polysulfone film is formed in a cylindrical shape, and the extract 6 is fixed from one side open end of any film. At a pressure (for example, 1 to 7 kg / cm ² ) under pressure, and ultrafiltration treatment can be performed through the membrane.

  Then, by performing ultrafiltration treatment with the ultrafiltration device 53, a solution containing a large amount of fucoidan components can be obtained as an ultrafiltration concentrate. At this time, many fibers can be removed, and a transparent ultrafiltration concentrate can be obtained.

(12) Bittern addition process (m)
The bittern addition process (m) is a process in which bittern N is added to the ultrafiltration concentrate subjected to the ultratreatment in the ultrafiltration process (l) described above, and final adjustment is performed.

  In this bittern addition step (m), a transparent final extract 54 in which the ultrafiltration concentrate and bittern N are evenly divided can be obtained.

Here, the concentration of bittern N to be added to the ultrafiltration concentrate 0.5% to 5%, preferably, can and Turkey be set to 2%.

And this final extract 54 contains a low-molecular fucoidan, can be used as a raw material for soft drinks, and is excellent in absorbability to the human body, so anticholesterol action, blood clearing action, Pharmacological effects such as anticoagulant action, anticancer action, anti-AIDS virus action, and anti-gastric ulcer action are also excellent.

  At this time, the fluidity of blood can be improved and the relaxation effect can be enhanced by the synergistic effect of the bittern containing mineral components and fucoidan.

  Examples will be described below with reference to FIGS. 1 and 2.

(1) Desalting treatment step (a)
In the desalting treatment step (a), mozuku 2 as seaweed soaked in the desalting / cleaning treatment tank 41 is transferred, and water from the water supply unit 43 is continuously fed into the desalting / washing treatment tank 41 by 20 By supplying the water for a minute and allowing only water to overflow from the desalting / washing tank 41, the salt content derived from seawater adhering to the mozuku 42 is desalted, and foreign matter and small animals adhering to the mozuku 42 Was removed.

(2) Immersion treatment process (b)
In the immersion treatment step (b), 100 liters of the aqueous solution 45 having a bittern concentration of 0.5% is accommodated in the immersion treatment vessel 44, and about 100 kg of mozuku 42 is contained as seaweed containing fucoidan in the aqueous solution 45. Immersion treatment was performed for 30 minutes at room temperature.

(3) Dehydration process (c)
In the dehydration treatment step (c), the desalted Moske 42 was transferred into the rotating tank 46 of the centrifuge, and the Moske 42 was dehydrated by rotating the rotating tank 46 for 10 minutes.

(4) Shredding process (d)
In the shredding step (d), the dehydrated Mozuku 3 was shredded to a size of 3 mm or less using the mixer 47. As a result, shredded mozuku 48 as shredded seaweed was obtained.

(5) Softening process (e)
In the softening treatment step (e), the shredded moscow 8 was pressurized at 1.6 atm and heated at 115 ° C., so that the shredded moscow 48 was swollen and softened.

  Then, it was naturally cooled to room temperature.

(6) Extraction process (g)
In the extraction process step (g), the softened shredded mosk 48 is repeatedly circulated in the extraction processor 1 about three times, so that the shredded mosk 48 is continuously pulverized and refined, and fucoidan is removed. Extract 6 containing was extracted.

(7) Concentration process (h)
The concentration step (h) is a step of evaporating the moisture of the extract 6 obtained in the extraction process step (g) by heating to 65 ° C. under reduced pressure using the concentration device 49.

  In this way, in the concentration step (h), the extremely fine insoluble fibrous polysaccharide contained in the refined extract 6 could be evenly distributed.

(8) Filtration process step (i)
In the filtration process step (i), the extract 6 extracted in the extraction process step (g) was filtered through a 200-mesh coarse filter 50.

(9) Low molecular weight treatment process (j)
In the low molecular weight treatment step (j), the extract 6 filtered through the coarse filter 50 was subjected to a low molecular weight treatment through the low molecular weight treatment device 51. At this time, the circulation treatment operation was repeated three times.

(10) Bittern addition process (k)
In the bittern addition step (k), bittern N is added to the nano-scale ultrafine insoluble fibrous polysaccharide that has been subjected to the molecular weight reduction treatment step (j) described above to a concentration of 2%. Thus, final adjustment was performed to obtain a final extract 52.

(11) Ultrafiltration process (l)
In the filtration process step (l), the extract 6 subjected to the extraction process in the above-described low molecular weight treatment process (j) was subjected to an ultrafiltration process by the ultrafiltration device 53.

(12) Bittern addition process (m)
In the bittern addition process (m), bittern N is added to the ultrafiltration concentrate subjected to the ultrafiltration in the ultrafiltration process (l) described above so as to have a concentration of 2%, and final adjustment is performed. A transparent final extract 54 was obtained in which the outer filtrate concentrate and bittern N were evenly separated.

  The final extract 54 contained a low-molecular fucoidan, but a large amount of fiber was removed.

[Evaluation]
(1) Final Extract 52 The amount of fucose in the final extract 52 obtained was measured by high performance liquid chromatography, and it was 48% by weight. Moreover, when the sulfuric acid content of the polysaccharide in the obtained final extract 52 was measured by the barium sulfate weight method, it was 1.19% by weight. The yield of fucoidan obtained was calculated based on the amount of mozuku used, and a high value of 12% was obtained. Moreover, when the average molecular weight (average weight molecular weight) of the fucoidan contained in the obtained final extract 52 was measured by gel filtration, it was found to be 30,000 to 150,000 which is within the preferable average molecular weight range. It could be confirmed.

(2) Final Extract 54 The amount of fucose in the final extract 54 obtained was measured by high performance liquid chromatography, and it was 48% by weight. Moreover, when the sulfuric acid content of the polysaccharide in the obtained final extract 54 was measured by the barium sulfate weight method, it was 1.19% by weight. The yield of fucoidan obtained was calculated based on the amount of mozuku used, and a high value of 12% was obtained. Moreover, when the average molecular weight (average weight molecular weight) of the fucoidan contained in the obtained final extract 54 was measured by gel filtration, it was found to be 30,000 to 80,000 which is within the preferable average molecular weight range. It could be confirmed.

  In addition, “purely bitter fucoidan drink” as the final extract 54 was ingested (drinked) by the subject when the bitter compounding ratio was 1%, 2%, 3%, 5%, and left of the subject The peripheral blood flow at the middle fingertip was measured before and after ingestion, respectively.

  Here, “purely fucoidan drink” was obtained by ingesting 30 ml diluted with 120 ml of water, and the peripheral blood flow at the left middle fingertip of the subject was measured every predetermined time. The results are as shown in the bar graph of FIG. 3 and the line graph of FIG.

  As apparent from FIGS. 3 and 4, when 1% bittern was added, the blood flow increased after 5 minutes, but after 10 minutes, it was the same as when 3% or 5% bittern was added. The behavior was shown.

  On the other hand, when the bittern was mixed with 2%, the peripheral blood flow increased with time.

  FIG. 5 shows the blood of one subject (27 years old, female) who ingested 2% bittern, before ingestion, 15 minutes after ingestion, 30 minutes after ingestion, and 60 minutes after ingestion. It was taken with a photomicrograph.

  As is clear from FIG. 5, since the fluidity of blood is continuously improved by ingesting “purely fucoidan drink”, such “purely fucoidan drink” It can be said that there is an effect.

  In addition, FIG. 6 shows a “purely bitter fucoidan drink” containing 25% fucoidan and 2% bittern, a 20-year-old subject (27 years old, female), a 30-year-old subject (36 years old, male) and a 40-year-old subject. It is a measurement result that (49 years old, male) ingested. The measurement time was measured between 10 am and 11:30 am.

  As is clear from FIG. 6, an increase in peripheral blood flow was observed over time regardless of the age of the subject.

  In addition, FIG. 7 shows an electroencephalogram measured by an electroencephalograph of a subject who ingested 2% bittern, before ingestion, 15 minutes after ingestion, 30 minutes after ingestion, and 60 minutes after ingestion. Is.

  As is clear from FIG. 5, it can be said that a relaxed effect can be obtained by taking a “purely fucoidan drink” because a significant expression of α waves is observed.

  Next, the configuration of the extract extraction processing apparatus A will be described in detail with reference to FIGS.

[Configuration of Extract Extraction Processing Apparatus A]
As shown in FIG. 8, the extract extraction processing apparatus A has a fluidity process via an inflow channel 2 on the upstream side of a flow channel forming casing 1 as an extraction processor that forms a channel from upstream to downstream. The processed product supply unit 4 for supplying the product 3 (in this embodiment, softened shredded seaweed 48) is connected to the downstream side of the flow channel forming casing 1 via the outflow channel 5 and the extract. A processed product collection unit 7 for collecting 6 is connected. P <b> 1 is a processed material pressure feed pump provided in the middle of the inflow channel 2.

  Then, as shown in FIG. 8, in the flow path forming casing 1, a number of flow dividing chambers 8 and merging chambers 9 are alternately arranged in the flow direction to flow down the flow path forming casing 1. The object 3 flows in the separation direction substantially orthogonal to the flow-down direction while meandering in the large number of flow dividing chambers 8, and is repeatedly divided in each flow dividing chamber 8, and flows down while meandering in the large number of merge chambers 9. The refined extract is extracted from the fluidity-treated product 3 while the flow flowing in the approach direction substantially orthogonal to the flow direction and repeatedly flowing in each merge chamber 9 is sequentially repeated in the flow-down direction. An extract extraction process is provided.

  Further, as shown in FIG. 18, a return flow path 10 is interposed between an inflow path 2 connected to the upstream side of the flow path forming casing 1 and an outflow path 5 connected to the downstream side. The extract extraction process performed in the flow path forming casing 1 can be repeated the required number of times.

  Here, the return channel 10 is connected to the inflow channel 2 through the upstream three-way valve 11, and is connected to the outflow channel 5 through the downstream three-way valve 12. P2 is provided, and the return flow path 10 communicates with the inflow / outflow flow paths 2 and 5 via the upstream and downstream three-way valves 11 and 12, and the return pump P2 provided in the middle of the return flow path 10 , The extract 6 is circulated in the flow path forming casing 1 so that the extract extraction process can be repeated.

  As shown in FIG. 8, a plurality of narrow channel forming grooves 13 extending in the direction in which the fluid treatment product flows are formed on the peripheral walls of the diversion chamber 8 and the merge chamber 9 at intervals in the circumferential direction. Yes.

  Hereinafter, the configuration of the above-described extract extraction processing apparatus A will be described more specifically with reference to FIGS. 9 to 16.

  That is, as shown in FIG. 9, the extract extraction processing apparatus A has a large number of flow dividing chambers 8 and merging chambers 9 arranged in the flow path forming casing 1 as described above. 1 is formed with flange-shaped connecting flange pieces 21 and 22 at the upper end edge and the lower end edge of a casing body 20 as a cylindrical processing body extending in the vertical direction. In addition, the weekly edge portions of the disk-shaped upper lid body 23 and the lower lid body 24 are brought into surface contact with each other and are detachably coupled by coupling bolts 25 and 26.

  Then, an inflow channel connecting port 27 for communicating with the inflow channel 2 is formed at the center of the upper lid 23, and for communicating with the outflow channel 5 at the center of the lower lid 24. An outflow channel connecting port portion 28 is formed.

  In addition, as shown in FIGS. 9 to 12, a large number of flow dividing chambers 8 and merge chambers 9 are arranged so as to face each other with the first chamber forming body 30 and the second chamber forming body 31 as one set. It is formed by.

  Then, as shown in FIGS. 10 (a) and 11 (a), the first chamber forming body 30 is formed on one side surface of the disk-shaped first support piece 32 in a polygonal cylindrical shape (in this embodiment). A plurality of first chamber forming pieces 33 each having a hexagonal shape are arranged in an aligned state (honeycomb state), and one side end face of each first chamber forming piece 33 is integrally formed continuously.

  Here, as shown in FIG. 9, the peripheral edge portion 32a of the first support piece 32 is formed to have an outer diameter close to the inner peripheral surface of the casing body 20 of the flow path forming casing 1, and is formed at the central portion. Forms a communication hole 34 communicating with the first chamber forming piece 33 located in the center.

  Further, as shown in FIGS. 10 (b) and 11 (b), the second chamber forming body 31 is formed on one side surface of the disk-like second support piece 35 in a polygonal cylindrical shape (in this embodiment). A plurality of second chamber forming pieces 36 having a hexagonal shape are arranged in an aligned state (honeycomb state), and one side end face of each second chamber forming piece 36 is integrally connected.

  Here, the peripheral edge 35a of the second support piece 35 is formed to have an outer diameter that is spaced from the inner peripheral surface of the casing body 20 of the flow path forming casing 1 by a predetermined width t.

  In addition, the first chamber forming piece 33 and the second chamber forming piece 36 are formed in the same size and in the same shape so that the center portions of the first support piece 32 and the second support piece 35 coincide with each other and are opposed to each other. 10, the second chamber forming pieces 36 are arranged so as to face the three adjacent first chamber forming pieces 33, 33, 33 evenly, as shown in FIG. The one chamber forming piece 33, 33, 33 and one second chamber forming piece 36 are communicated with each other.

  In this manner, the first chamber forming body 30 is arranged in a state where the first chamber forming piece 33 is suspended from the first support piece 32 at the uppermost portion in the flow path forming casing 1, and the first chamber forming piece is also provided. The second chamber forming body 31 is disposed in a state where the second chamber forming piece 36 is opposed to the lower portion 33 from below.

  Then, the second support piece 35 of the second chamber formation body 31 of the second set is placed in surface contact with the second support piece 35 of the second chamber formation body 31 from below. The two chamber forming pieces 36 are arranged in a suspended state, and the first chamber forming body 30 is arranged with the first chamber forming pieces 33 opposed to the second chamber forming pieces 36 from below.

  Thereafter, the third set of the first chamber forming body 30 and the second chamber forming body 31 are arranged in the same manner as the first set, and the fourth set of the first chamber forming body 30 and the second chamber forming body 31 are arranged in two. The stacking operation of arranging in the same manner as the set is repeated (in the present embodiment, up to the sixth set).

  At this time, the communication hole 34 of the first chamber forming body 30 disposed at the uppermost stage is communicated with the inflow channel connecting port portion 27 of the upper lid 23, while the communication of the first chamber forming body 30 disposed at the lowermost stage is performed. The hole 34 is communicated with the outflow channel connecting port portion 28 of the lower lid 24, and the center portions of the first and second chamber forming bodies 30 and 31 are aligned with the axis of the channel forming casing 1.

  A ring-shaped moving flow path 37 is formed between the peripheral portions 35a and 35a of the second support pieces 35 and 35 that are stacked in a face-to-face state and the inner peripheral surface of the casing body 20. .

  In this way, when the fluid processed material 3 is caused to flow into the processed material supply section 4 → the inflow channel 8 → the inflow channel connection port 27 → the uppermost communication hole 34, as shown in FIG. The first chamber forming piece 33 → the second chamber forming piece 36 → the first chamber forming piece 33 → the second chamber forming piece 36 while meandering and substantially perpendicular to the flow direction (vertical downward direction in the present embodiment) In this embodiment, the fluidized product 3 is repeatedly diverted by flowing in the separating direction (substantially horizontal outward direction in the present embodiment).

  As a result, a number of flow dividing chambers 8 are formed by the first chamber forming piece 33 of the first chamber forming body 30 and the second chamber forming piece 36 of the second chamber forming body 31 in the uppermost set. become.

  The fluid processed product 3 that has reached the outer peripheral edge portion passes through the moving flow path 37 and the first chamber forming piece 33 on the peripheral edge side provided in the second chamber forming body 31 and the second chamber forming body 31 in the second set. → the second chamber forming piece 36 → the first chamber forming piece 33 → the second chamber forming piece 36, meandering in the approach direction (this embodiment) substantially orthogonal to the flow direction (vertical down direction in this embodiment) In this case, the fluid processed product 3 is repeatedly joined together.

  As a result, the multiple chambers 9 are formed by the first chamber forming piece 33 of the second chamber forming body 30 and the second chamber forming piece 36 of the second chamber forming body 31.

  While such splitting and merging are sequentially repeated in the downstream direction, the physically refined extract can be extracted from the fluid processed product 3.

  In other words, as described above, the fluid processed product 3 is formed by collision at right angles to the bottom and side walls of the first chamber forming bodies 30 and the second chamber forming bodies 31, and to form the first chamber forming pieces 33 and the second chambers. Hydrodynamic shearing by vortex mixing by splitting, merging, meandering between the pieces 36, and inflow between the plurality of first chamber forming pieces 33 and the second chamber forming pieces 36, and each first chamber forming piece 33 And the upper edge of each of the first and second chamber forming pieces 33, 36, hydrodynamic shearing when passing through an orifice which is a communication path between the first chamber forming piece 36 and each second chamber forming piece 36, Mixing by shearing, mechanical cavitation, and the like when passing through the container is performed.

  Here, the total number of shunts is determined by the number of chambers of the first and second chamber forming pieces 33 and 36 that are sequentially arranged radially from the center. For example, it may be hexagonal in plan view shown in FIG. For example, there are three rows of first chamber forming bodies 30 with 6 rooms, 12 rooms, and 18 rooms (36 rooms in total), and 3 rows with 3 rooms, 9 rooms, and 15 rooms (27 rooms in total). In the case where the second chamber forming body 31 is polymerized, the total number of divided flows reaches several thousand times.

  The total number of the diversions means that the diversion chamber 8 formed by the first chamber formation piece 33 and the second chamber formation pieces 36 that are in communication with each other in the first chamber formation body 30 and the second chamber formation body 31. In some cases, the number of flow dividing chambers 8 is a product of the number of flow dividing chambers 8 and the number of flow dividing chambers 8. It can be appropriately increased or decreased by increasing or decreasing the number of rows of the second chamber forming pieces 36.

  In the configuration as described above, in the present embodiment, as shown in FIG. 13, a plurality of narrow channels extending in the direction in which the fluid treatment product 3 flows is formed on the inner peripheral wall of the first chamber forming piece 33. The formation grooves 13 are formed at intervals in the circumferential direction.

  That is, in this embodiment, the narrow channel forming groove 13 is formed on the inner peripheral wall of the first chamber forming piece 33 on the axis of each of the first and second chamber forming pieces 33 and 36 as shown in FIG. A groove having a V-shaped cross section extending in the direction is formed continuously in the circumferential direction, and the fluid processed material 3 moves in the first and second chamber forming pieces 33 and 36 while meandering. In the narrow flow path forming groove 13 having a V-shaped cross section, the area in contact with the fluid processed product 3 is particularly increased.

  Further, the narrow channel forming groove 13 is formed on the inner peripheral wall of the second chamber forming piece 36 in the same manner as the first chamber forming piece 33.

  In this way, a plurality of narrow flow path forming grooves 13 extending in the direction in which the flowable product 3 flows are formed in the circumferential walls of the flow dividing chamber 8 and the merge chamber 9 at intervals in the circumferential direction. Therefore, the fluid processed product 3 is physically and forcibly shredded when being shunted through the narrow channel forming groove 13 in the shunting chamber 8, and the narrow channel in the joining chamber 9. When merged through the formation groove 13, an extract that is physically and forcibly shredded and further refined is extracted.

  That is, the tip peripheral edge of the narrow channel forming groove 13 having a V-shaped cross section is used as an orifice, hydrodynamic shearing when the fluid processed material 3 passes, pulverization due to impact breakage, Mixing by efficient cavitation or the like is performed efficiently.

  Therefore, for example, when seaweed or the like containing fucoidan is flowed as the fluid processed product 3 to the extract extraction processing apparatus A according to the present invention, the fucoidan molecule contained in the seaweed or the like is physically Since fucoidan that has been cut into a low molecular weight is easily and reliably extracted as an extract, the extraction efficiency of fucoidan can be increased.

  FIG. 15 is an enlarged cross-sectional view of the narrow flow path forming groove 13 as a first modification. The narrow flow path forming groove 13 is formed in a quadrangular cross section and is in contact with the fluid processed product 3. So that the fluidized product 3 passes through the hydrodynamic shear, the pulverization by impact destruction, and the mixing by mechanical cavitation and the like are performed more efficiently. ing.

  FIG. 16 is an enlarged cross-sectional view of a narrow flow path forming groove 13 as a modified example 2. The narrow flow path forming groove 13 is formed in an inverted trapezoidal cross section so that the fluid processed product 3 By further increasing the contact area, hydrodynamic shearing when the fluidized product 3 passes, grinding by impact fracture, and mixing by mechanical cavitation and the like are performed more efficiently. It is supposed to be.

  In the present embodiment, a return channel 10 is provided between the inflow channel 2 connected to the upstream side of the channel-forming casing 1 and the outflow channel 5 connected to the downstream side, so that The extract extraction process performed in the path forming casing 1 can be repeated the required number of times.

  That is, as described above, the upstream three-way valve 11 that communicates and connects the inflow channel 2 and the return channel 10 is operated to bring the inflow channel 2 and the return channel 10 into a non-communication state. By operating the downstream side three-way valve 12 that connects the channel 5 and the return channel 10 in communication, the outflow channel 5 and the return channel 10 are brought into a non-communication state. 4, the fluid processed material 3 is caused to flow into the flow channel forming casing 1 through the inflow channel 2, the extract extraction process is performed in the flow channel forming casing 1, and then the flowed product 3 is discharged through the outflow channel 5. It can be recovered in the processed product recovery unit 7.

  Moreover, when performing the extract extraction process in the flow path forming casing 1 a plurality of times, while the first extract extraction process is being performed in the flow path forming casing 1, The upstream three-way valve 11 that connects the return flow path 10 and the return flow path 10 is operated to bring the inflow flow path 2 and the return flow path 10 into communication, and the outflow flow path 5 and the return flow path 10 are connected to each other. By operating the downstream three-way valve 12 to bring the outflow channel 5 and the return channel 10 into communication, the extract 6 that has undergone the extract extraction process in the channel-forming casing 1 is returned. It can be returned to the flow path forming casing 1 again through the flow path 10.

  Then, after repeating the required number of extract extraction processing steps, the downstream three-way valve 12 is operated to bring the outflow passage 5 and the return passage 10 into a non-communication state, thereby forming the passage-forming casing 1. The extract 6 that has been subjected to the extract extraction process within the required number of times can be discharged through the outflow passage 5 and recovered in the processed product recovery section 7.

  In this way, since the extract extraction process can be repeated a required number of times, for example, when the fluid processed product 3 is seaweed containing fucoidan, fucoidan as an extract is extracted. The average molecular weight can be easily set to a desired molecular weight (for example, 10,000 to 5,000,000, preferably 60,000 or less), and an extract with the set molecular weight can be obtained with high accuracy. .

  Further, in the present embodiment, the first chamber forming piece 33 and the second chamber forming piece 36 are shown in a hexagonal shape in plan view, but are not limited to such shapes. The plan view shapes of the first chamber forming piece 33 and the second chamber forming piece 36 are shown in FIG. 17 as a triangle shown as modification example 1, FIG. 18 as a square shown as modification example 2, FIG. It can also be formed.

  In the present embodiment, the outer diameter of the first support piece 32 having a large diameter is brought into close contact with the inner peripheral surface of the casing body 20 to maintain the sealing function. A sealing member (not shown) such as an O-ring having heat resistance may be interposed between the peripheral surface and the outer peripheral edge of the first support piece 32 to maintain the sealing function.

The process explanatory drawing of the processing method of the front | former stage of the seaweed containing the fucoidan which concerns on this invention. The process explanatory drawing of the processing method of the back | latter stage of the seaweed containing the fucoidan which concerns on this invention. The bar graph which shows a blood flow measurement result. The line graph which shows a blood flow measurement result. The micrograph which shows the change of a test subject's blood flow over time. The line graph which shows a blood flow measurement result. The graph which shows the time-dependent change of a test subject's electroencephalogram. Cross-sectional explanatory drawing of the extract extraction processing apparatus which concerns on this invention. Cross-sectional explanatory drawing of the flow-path formation casing of the extract extraction processing apparatus. The front view (a) of a 1st chamber formation body, and the front view (b) of a 2nd chamber formation body. The perspective view (a) of a 1st chamber formation body, and the perspective view (b) of a 2nd chamber formation body. The II sectional view taken on the line of FIG. The expanded sectional view of the 1st chamber formation piece. The partial expanded sectional view of the 1st chamber formation piece. The partial expansion sectional view of the 1st chamber formation piece as modification example 1. FIG. The partially expanded sectional view of the 1st chamber formation piece as the modification example 2. FIG. The front explanatory view of the state where the 1st chamber formation object and the 2nd chamber formation object as change example 1 were arranged by superposition, and the division room or the confluence room was formed. The cross-sectional front explanatory drawing of the state which superposedly arrange | positioned the 1st chamber formation body as the modification example 2, and the 2nd chamber formation body, and formed the shunt chamber or the confluence | merging chamber. The front explanatory view of the state where the 1st chamber formation object and the 2nd chamber formation object as change example 3 were superposed, and the diversion room or the confluence room was formed.

Explanation of symbols

A Extract Extraction Processing Device 1 Channel Forming Casing 2 Inflow Channel 3 Flowable Processed Material 4 Processed Product Supply Unit 5 Outflow Channel 6 Extraction 7 Processed Product Recovery Unit 8 Dividing Chamber 9 Junction Chamber
10 Return flow path
41 Desalination / cleaning tank
42 Seaweed
43 Water supply section
44 Dipping container
45 Aqueous solution
46 Rotating tank
47 Mixer
48 Shredded seaweed
49 Extraction processor
49 Concentrator
50 Coarse filter
51 Low molecular processor
52 Final extract
53 Ultrafiltration equipment
54 Final extract

Claims (3)

A desalting / washing process that removes salt from seawater adhering to seaweeds and removes foreign substances and small animals adhering to seaweeds and washing them;
The seaweed desalted and washed in the desalting / washing process is immersed in an aqueous solution added with bittern, so that the viscosity of alginic acid contained in the seaweed by minerals such as Mg ions contained in the bittern. An immersion treatment process for suppressing
A dehydration treatment step of dehydrating the seaweed soaked in the immersion treatment step;
A shredding process for shredding seaweed dehydrated in the dewatering process using a shredding device;
A softening treatment step of swelling and softening the shredded seaweed by pressurizing and heating or immersing the shredded seaweed shredded in the shredding treatment step;
Extraction that extracts the fucoidan-containing extract while continuously pulverizing and pulverizing the shredded seaweed by distributing the shredded seaweed softened in the softening treatment step in the extraction processor. Processing steps;
The molecular weight reduction treatment step of subjecting the extract extracted in the extraction treatment step to a low molecular weight treatment through a low molecular weight treatment device having the same configuration as the extraction treatment device;
Adding bittern to the ultrafine insoluble fibrous polysaccharide subjected to low molecular weight treatment in the low molecular weight treatment step, and performing the final adjustment,
A method for treating seaweed containing fucoidan, comprising: The extraction processor is:
A flow path forming casing;
A large number of cylindrical first chamber forming pieces are formed on one side surface of a plate-like first support piece which has an outer diameter close to the inner peripheral surface of the flow path forming casing and has a communication hole in the center. A first chamber forming body arranged in an aligned state and continuously arranged;
A large number of cylindrical second chamber forming pieces are aligned on one side surface of the plate-like second support piece, which is formed to have an outer diameter with a certain width from the inner peripheral surface of the flow path forming casing. A second chamber forming body arranged in a row and connected,
Comprising
The first and second chamber forming bodies of the first set and the first and second chamber forming bodies of the second set are stacked in an interview state in the flow path forming casing, and each forming body has the same size. In addition, the first support piece and the second support piece formed in the same shape are arranged so as to face the three first chamber forming pieces adjacent to each other so that one second chamber forming piece is evenly straddled. Communicating one chamber forming piece with one second chamber forming piece;
A ring-shaped moving flow path is formed between the peripheral portions of the second support pieces of the first set and the second set stacked and the inner peripheral surface of the flow path forming casing,
The shredded seaweed is provided in the first chamber forming body and the second chamber forming body in the first set from the communication hole. The first chamber forming piece on the center side → the second chamber forming piece → the first chamber forming piece → the first A two-chamber forming piece that flows in a separating direction substantially perpendicular to the flow-down direction while meandering, such as a two-chamber forming piece, is shunted repeatedly, and the shredded seaweed that has reached the outer peripheral edge passes through the moving channel and forms a second set of first chamber-forming bodies And the first chamber forming piece on the peripheral side provided in the second chamber forming body → the second chamber forming piece → the first chamber forming piece → the second chamber forming piece, and the approach direction substantially orthogonal to the flow-down direction while meandering It is characterized by the fact that the extract containing fucoidan is extracted from shredded seaweed, and is repeatedly combined.
The processing method of the seaweed containing fucoidan according to claim 1, wherein the processing is performed by the extraction processor . The extraction processor is provided with a narrow channel forming groove formed by continuously forming a groove extending in the axial direction on the inner peripheral wall of the first and second chamber forming pieces in the circumferential direction. In the narrow channel forming groove when flowing in the first and second chamber forming pieces while the seaweed meanders, the area in contact with the shredded seaweed is increased,
The method for treating seaweed containing fucoidan according to claim 2, wherein the treatment is performed by the extraction processor .

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