JP4545622B2 - Luminescence measuring device - Google Patents

Description

  The present invention relates to a luminescence measuring apparatus that detects light generated in a sample in a sample cell.

The luminescence measuring device analyzes the sample by irradiating the sample containing the fluorescent material with excitation light and detecting the fluorescence generated in the sample, or detecting the chemiluminescence generated in the sample. (See Patent Document 1).
Japanese Patent No. 3183863

  However, in the conventional luminescence measuring device, even if a sample is analyzed based on the measurement result of fluorescence or chemiluminescence, the analysis may not be performed with high accuracy. The present invention has been made to solve the above-described problems, and an object of the present invention is to provide a luminescence measuring apparatus capable of analyzing a sample with high accuracy.

  The luminescence measuring apparatus according to the present invention includes (1-1) a first excitation unit that irradiates a sample in the first sample cell with excitation light, and (1-2) light generated in the sample in the first sample cell. A first detection unit for detecting; (1-3) a first injection unit for injecting a reagent into the first sample cell; and (2-1) a second excitation for irradiating the sample in the second sample cell with excitation light. (2-2) a second detection unit for detecting light generated in the sample in the second sample cell, (2-3) a second injection unit for injecting a reagent into the second sample cell, ( 3-1) a third excitation unit that irradiates the sample in the third sample cell with excitation light; (3-2) a third detection unit that detects light generated in the sample in the third sample cell; -3) a third injection unit for injecting a reagent into the third sample cell, (4-1) a fourth excitation unit for irradiating the sample in the fourth sample cell with excitation light, and (4-2) a fourth A fourth detector for detecting light generated in the sample in the sample cell; and (4-3) injecting a reagent into the fourth sample cell. A fourth injection unit, and (5) a control unit that performs control such that any two or more detection units among the first detection unit, the second detection unit, the third detection unit, and the fourth detection unit detect light simultaneously. And.

  Furthermore, the luminescence measuring apparatus according to the present invention has the following characteristics. That is, the optical systems of the first excitation unit, the second excitation unit, the third excitation unit, and the fourth excitation unit have the same configuration. The optical systems of the first detection unit, the second detection unit, the third detection unit, and the fourth detection unit have the same configuration. The first excitation unit, the second excitation unit, the third excitation unit, the fourth excitation unit, the first detection unit, the second detection unit, the third detection unit, and the fourth detection unit are the first sample cell and the second sample cell. , Arranged around the third sample cell and the fourth sample cell. The optical axes of the optical systems of the first excitation unit and the first detection unit in the first sample cell are orthogonal to each other, and the optical axes of the optical systems of the second excitation unit and the second detection unit in the second sample cell are The optical axes of the optical systems of the third excitation unit and the third detection unit in the third sample cell are orthogonal to each other, and the optical systems of the fourth excitation unit and the fourth detection unit in the fourth sample cell. These optical axes are orthogonal to each other. The optical axes of the optical systems of the first excitation unit, the second excitation unit, the third excitation unit, the fourth excitation unit, the first detection unit, the second detection unit, the third detection unit, and the fourth detection unit are as follows: It is parallel to either the first direction or the second direction orthogonal to each other.

  The luminescence measuring apparatus according to the present invention includes a first stirring unit for stirring the sample in the first sample cell, a second stirring unit for stirring the sample in the second sample cell, and a sample in the third sample cell. It is preferable to further include a third stirring unit for stirring the sample and a fourth stirring unit for stirring the sample in the fourth sample cell. In this case, for example, it is effective when the sample contains cells.

  In addition, the luminescence measurement device according to the present invention preferably further includes a display unit that displays the status of light detection by each of the first detection unit, the second detection unit, the third detection unit, and the fourth detection unit in real time. It is. Moreover, in the luminescence measuring apparatus according to the present invention, the control unit is any one of the first detection unit, the second detection unit, the third detection unit, and the fourth detection unit based on the light detection result by any one of the detection units. It is preferable to subtract the light detection result by the detection unit.

  According to the present invention, a sample can be analyzed with high accuracy.

  The best mode for carrying out the present invention will be described below in detail with reference to the accompanying drawings. In the description of the drawings, the same elements are denoted by the same reference numerals, and redundant description is omitted. For convenience of explanation, an xyz rectangular coordinate system is set and shown in the figure.

  FIG. 1 is a configuration diagram of a luminescence measuring apparatus 1 according to the present embodiment. The luminescence measuring apparatus 1 shown in this figure includes a first unit 10 including a first excitation unit 11 and a first detection unit 12, a second unit 20 including a second excitation unit 21 and a second detection unit 22, and a third excitation. A third unit 30 including the unit 31 and the third detection unit 32, and a fourth unit 40 including the fourth excitation unit 41 and the fourth detection unit 42. The luminescence measurement device 1 also includes a control unit 50 and a display unit 60.

  The first excitation unit 11 included in the first unit 10 irradiates the sample in the first sample cell 19 with excitation light. This excitation light is light having a wavelength that can excite the fluorescent substance contained in the sample in the first sample cell 19. The first detection unit 12 included in the first unit 10 detects light (fluorescence, chemiluminescence, etc.) generated in the sample in the first sample cell 19.

  The second excitation unit 21 included in the second unit 20 irradiates the sample in the second sample cell 29 with excitation light. This excitation light is light having a wavelength that can excite the fluorescent substance contained in the sample in the second sample cell 29. Further, the second detection unit 22 included in the second unit 20 detects light (fluorescence, chemiluminescence, etc.) generated in the sample in the second sample cell 29.

  The third excitation unit 31 included in the third unit 30 irradiates the sample in the third sample cell 39 with excitation light. This excitation light is light having a wavelength that can excite the fluorescent substance contained in the sample in the third sample cell 39. The third detection unit 32 included in the third unit 30 detects light (fluorescence, chemiluminescence, etc.) generated in the sample in the third sample cell 39.

  The fourth excitation unit 41 included in the fourth unit 40 irradiates the sample in the fourth sample cell 49 with excitation light. This excitation light is light having a wavelength that can excite the fluorescent substance contained in the sample in the fourth sample cell 49. The fourth detection unit 42 included in the fourth unit 40 detects light (fluorescence, chemiluminescence, etc.) generated in the sample in the fourth sample cell 49.

The optical systems of the first excitation unit 11, the second excitation unit 21, the third excitation unit 31, and the fourth excitation unit 41 have the same configuration. The optical systems of the first detection unit 12, the second detection unit 22, the third detection unit 32, and the fourth detection unit 42 also have the same configuration. The first sample cell 19, the second sample cell 29, the third sample cell 39, and the fourth sample cell 49 are each a rectangular parallelepiped shape, and are arranged at regular intervals in the x direction and the y direction. Around the first sample cell 19, the second sample cell 29, the third sample cell 39, and the fourth sample cell 49, the first excitation unit 11, the second excitation unit 21, the third excitation unit 31, and the fourth excitation unit. 41, the 1st detection part 12, the 2nd detection part 22, the 3rd detection part 32, and the 4th detection part 42 are arrange | positioned.
In the first sample cell 19, the optical axes of the optical systems of the first excitation unit 11 and the first detection unit 12 are orthogonal to each other. In the second sample cell 29, the optical axes of the optical systems of the second excitation unit 21 and the second detection unit 22 are orthogonal to each other. In the third sample cell 39, the optical axes of the third excitation unit 31 and the third detection unit 32 are orthogonal to each other. Further, in the fourth sample cell 49, the optical axes of the optical systems of the fourth excitation unit 41 and the fourth detection unit 42 are orthogonal to each other.

  Optics of the first excitation unit 11, the second excitation unit 21, the third excitation unit 31, the fourth excitation unit 41, the first detection unit 12, the second detection unit 22, the third detection unit 32, and the fourth detection unit 42, respectively. The optical axis of the system is parallel to either the x-axis direction or the y-axis direction orthogonal to each other. That is, the excitation light output from the first excitation unit 11 and applied to the sample in the first sample cell 19 is The light that travels in the −y direction and is generated by the sample in the first sample cell 19 and detected by the first detector 12 travels in the + x direction. The excitation light output from the second excitation unit 21 and applied to the sample in the second sample cell 29 travels in the −y direction, is generated in the sample in the second sample cell 29, and is detected by the second detection unit 22. Light travels in the -x direction. Excitation light output from the third excitation unit 31 and applied to the sample in the third sample cell 39 proceeds in the + y direction, is generated in the sample in the third sample cell 39, and is detected by the third detection unit 32. Light travels in the -x direction. Further, the excitation light output from the fourth excitation unit 41 and applied to the sample in the fourth sample cell 49 travels in the + y direction, is generated in the sample in the fourth sample cell 49, and is detected by the fourth detection unit 42. Light travels in the + x direction.

  The control unit 50 performs control such that any two or more detection units among the first detection unit 12, the second detection unit 22, the third detection unit 32, and the fourth detection unit 42 simultaneously detect light. That is, the control unit 50 controls the excitation light output in each of the first excitation unit 11, the second excitation unit 21, the third excitation unit 31, and the fourth excitation unit 41, and the first detection unit 12 and the second detection unit. 22, the light detection operation in each of the third detection unit 32 and the fourth detection unit 42 is controlled. The control unit 50 acquires the status of light detection and the detection results by the first detection unit 12, the second detection unit 22, the third detection unit 32, and the fourth detection unit 42, and sends them to the display unit 60 in real time. Display. In addition, the control unit 5 uses any one of the first detection unit 12, the second detection unit 22, the third detection unit 32, and the fourth detection unit 42 based on the light detection result of any one of the detection units. The light detection result is subtracted, and the subtraction result is displayed on the display unit 60.

  FIG. 2 is a configuration diagram of the first unit 10 included in the luminescence measurement apparatus 1 according to the present embodiment. The first unit 10 includes the first excitation unit 11 and the first detection unit 12 described above, and also includes a first stirring unit 13 and a first temperature control unit 14.

  The first excitation unit 11 includes a driver 111, a light emitting element 112, a lens 113, and an optical filter 114. The driver 111 is controlled by the control unit 50 and outputs a drive current to be supplied to the light emitting element 112. The light emitting element 112 inputs a driving current output from the driver 111 and outputs excitation light, and is preferably a light emitting diode or a laser diode. The lens 113 converges the light output from the light emitting element 112 and collects the excitation light in the first sample cell 19. The optical filter 114 selectively transmits excitation light having a wavelength capable of exciting the fluorescent substance in the sample out of the light output from the light emitting element 112. Note that in the case where a laser diode is used as the light emitting element 112 and the monochromaticity of light output from the light emitting element 112 is excellent, the optical filter 114 is unnecessary. The optical axis of the optical system from the light emitting element 112 to the first sample cell 19 is parallel to the y axis.

  The first detection unit 12 includes a lens 121, an optical filter 122, and a photodetector 123. The lens 121 inputs light generated by the sample in the first sample cell 19 and collimates this light. The optical filter 122 selectively blocks the scattering component of the excitation light out of the light reaching from the sample in the first sample cell 19 and transmits light of other wavelengths (fluorescence or chemiluminescence). The photodetector 123 receives the light transmitted through the optical filter 122, detects the intensity of the received light, and notifies the control unit 50 of the detection result. The photodetector 123 preferably includes a photomultiplier tube, and preferably can perform photon counting. The optical axis of the optical system from the first sample cell 19 to the photodetector 123 is parallel to the x-axis.

  The first stirring unit 13 includes a stirrer 131 and a stirring control unit 132. The stirrer 131 stirs the sample in the first sample cell 19 under the control of the stirring control unit 132. The first temperature adjustment unit 14 includes a temperature sensor 141, a heater 142, and a temperature control unit 143. The temperature sensor 14 measures the temperature of the sample in the first sample cell 19. The heater 142 maintains the temperature of the sample in the first sample cell 19 at a desired value under the control of the temperature control unit 143 performed based on the temperature measurement result.

  FIG. 3 is a perspective view of the first sample cell 19. The first sample cell 19 includes a sample cell holder 191 and a cuvette 192 that is a rectangular parallelepiped transparent container, and is arranged so that each side wall is parallel to either the xz plane or the yz plane. The sample cell holder 191 is provided with a stirrer 131 at the bottom, open at the top, and capable of inserting a cuvette 192. Further, the sample cell holder 191 has an incident window 193 for allowing excitation light to enter the inside on the side wall on the + y side, and an exit window 194 for emitting the light generated inside to the outside on the side wall on the + x side. .

  FIG. 4 is a partial cross-sectional view showing the configuration of the first excitation unit 11. The light emitting element 112 included in the first excitation unit 11 is fitted into the opening of the light emitting element holder 115. The optical filter 114 is fitted into the opening of the optical filter holder 116. The light output from the light emitting element 112 is irradiated to the sample in the first sample cell 19 through the opening of the light emitting element holder 115 and further through the opening of the optical filter holder 116 and the optical filter 114.

  FIG. 5 is a partial cross-sectional view showing the configuration of the first detection unit 12. The optical filter 122 included in the first detection unit 12 is fitted into the opening of the holder 124. The photodetector 123 includes a photomultiplier tube 125, a bleeder resistance circuit 126, a discriminator 127, a high-voltage power supply 128, and a connector 129, which are housed in a shield box. The photomultiplier tube 125 is given a predetermined voltage value to the multistage dynode by the bleeder resistance circuit 126, and the photoelectron generated by the incidence of the photon on the photocathode is multiplied by the multistage dynode, and the multiplication is performed. A current pulse corresponding to the electrons is output to the discriminator 127. The bleeder resistance circuit 126 resistance-divides the high voltage output from the high-voltage power supply 128 and gives a predetermined voltage value after the resistance division to the multistage dynodes of the photomultiplier tube 125. The discriminator 127 receives the current pulse output from the photomultiplier tube 125 and discriminates the pulse height. When light transmitted through the optical filter 122 out of the light generated in the first sample cell 19 enters the photocathode of the photomultiplier tube 125, photoelectrons are generated from the photocathode. The photoelectrons are multiplied by the multistage dynodes of the photomultiplier tube 125, and a current pulse corresponding to the multiplied electrons is output from the photomultiplier tube 125 and input to the discriminator 127. The current pulse is discriminated by the discriminator 127 and output from the connector 129 to the control unit 50.

  The first unit 10 has been described above with reference to FIGS. 2 to 5, but each of the second unit 20, the third unit 30, and the fourth unit 40 has the same configuration as the first unit 10. . That is, each of the second unit 20, the third unit 30, and the fourth unit 40 includes a stirring unit (having the same configuration as the first stirring unit 13) that stirs the sample in the corresponding sample cell. Including a temperature control unit (same configuration as the first temperature control unit 14) for adjusting the temperature in the cell, each excitation unit 21, 31, 41 has the same configuration as the first excitation unit 11, Each of the detection units 22, 32, and 42 has the same configuration as that of the first detection unit 12.

  FIG. 6 is a perspective view of the luminescence measuring apparatus 1 according to the present embodiment. As shown in this figure, on the substrate 2, the light emitting element holder 115, the optical filter holder 116, the photodetector 123, the holder 124, the first sample cell 19, and the second unit 20 included in the first unit 10. Light-emitting element holder 215, optical filter holder 216, photodetector 223, holder 224, second sample cell 29, light-emitting element holder 315 included in the third unit 30, optical filter holder 316, light detection 323, holder 324 and third sample cell 39, light emitting element holder 415, optical filter holder 416, photodetector 423, holder 424 and fourth sample cell 49 included in the fourth unit 40, and control unit 50 (Part of) is arranged with a fixed relative positional relationship.

  Each of the light emitting element holders 215, 315, and 415 has the same configuration as the light emitting element holder 115 described above. Each of the optical filter holders 216, 316 and 416 has the same configuration as the optical filter holder 116 described above. Each of the photodetectors 223, 323, and 423 has the same configuration as the photodetector 123 described above. Each of the holders 224, 324, and 424 has the same configuration as the holder 124 described above. The control unit 50 (a part of) disposed on the base 2 is a part that processes data output from each of the photodetectors 123, 223, 323, and 423, for example.

  FIG. 7 is a perspective view of the luminescence measuring apparatus 1 according to the present embodiment. In this figure, in addition to the structure shown in FIG. 6, the upper lid 3, the first injection part 15, the second injection part 25, the third injection part 35 and the fourth injection part 45 are shown. The upper lid 3 covers the first unit 10, the second unit 20, the third unit 30 and the fourth unit 40 from above the base 2. In this covered state, intrusion of ambient light into the space surrounded by the base 2 and the upper lid 3 is blocked. In this covered state, the first injection unit 15 can inject a reagent into the first sample cell 19, and the second injection unit 25 can inject a reagent into the second sample cell 29. The third injection unit 35 can inject a reagent into the third sample cell 39, and the fourth injection unit 45 can inject a reagent into the fourth sample cell 49.

  Each of the injection parts 15, 25, 35, 45 preferably includes a syringe that is detachable with respect to the upper lid 3. In this case, the reagent is aspirated when removed from the upper lid 3, and the upper lid The reagent can be injected into the corresponding sample cell when it is attached to 3. The reagent to be injected by each of the injection units 15, 25, 35, 45 is, for example, a stimulant for inducing some reaction to the sample in the corresponding sample cell.

  Next, an example of measurement using the luminescence measurement apparatus 1 according to the present embodiment will be described. Here, a case where fluorescence and chemiluminescence generated from a human neutrophil-like cell as a sample are simultaneously measured will be described. Each of the three sample cells 19, 29, 39 contains an aqueous solution of human neutrophil-like cells as a sample, and the other one sample cell 40 contains only a solvent (generally water) as a sample.

Here, the human neutrophil-like cell is RPMI medium containing 10% serum containing 0.5 mM Bt ₂ cAMP (dibutyryl cyclic adenosine phosphate) as a human monocytic cell line cell THP-1, and the cell density is 3 × 10 ^5. The cells / ml were cultured for 3 days at 37 ° C. in the presence of 5% CO ₂ . The sample neutrophil-like cell is preliminarily prepared as a calcium fluorescent indicator fluo-3 (1- [2-amino-5- (2,7-dichloro-6-hydroxy-3-oxy-9-xanthenyl) phenoxyl]- 2- (2-amino-5-methylphenoxy) ethane-N, N, N ', N'-tetraaceticacid), and chemiluminescent reagent CLA (Citrus luciferin derivative, 2-methyl-6-phenyl-3, 7-dihydroimidazo [1,2-a] pyrazin-3-one) is added to form a suspension.

  Each of the sample cells 19, 29, 39 containing such samples is set at a predetermined position. A sample cell 49 containing only the solvent is also set at a predetermined position. The samples contained in the sample cells 19, 29, 39, and 49 are stirred by the stirring unit and maintained at a constant temperature of 37 ° C. by the temperature control unit.

  When the above measurement preparation is completed, pulsed excitation light (wavelength 488 nm) is output from each of the excitation units 11, 21, 31, 41 at a constant repetition period (about milliseconds), and the excitation light is output from the sample cells 19, 29. , 39, 49 are irradiated on the sample. The light generated in the sample in each of the sample cells 19, 29, 39, 49 is detected by the detection units 12, 22, 32, 42, and a signal having a value corresponding to the detected light intensity is transmitted to the control unit. 50 is output.

  When detecting light generated from the sample in each of the sample cells 19, 29, and 39, fluorescence, chemiluminescence, and Raman scattered light are detected when the excitation light is irradiated, and chemiluminescence is detected when the excitation light is not irradiated. . On the other hand, when detecting light generated in the sample in the sample cell 49, Raman scattered light is detected when the excitation light is irradiated.

  Here, the fluorescence is light generated by irradiating excitation light to a complex formed by binding free calcium ions in a neutrophil-like cell and the calcium fluorescent indicator fluo-3, and its intensity is neutrophil. This represents the concentration of free calcium ions in the sphere-like cell, and the wavelength is 530 nm. Chemiluminescence is light generated when superoxide produced by human neutrophils reacts with chemiluminescent reagent CLA to produce CLA oxide, and its intensity represents the amount of superoxide produced The wavelength is 380 nm. Further, the Raman scattered light is light caused by the Raman scattering phenomenon accompanying the excitation light irradiation to the solvent in each sample cell, and the wavelength thereof is around 530 nm. Since the wavelengths of the fluorescent light and the Raman scattered light are close to each other, they cannot be separated immediately.

  The signals output from the light detection units 12, 22, 32, and 42 are input to the control unit 50 and processed as follows. First, the signal value output from each of the light detection units 12, 22, and 32 at the time of excitation light irradiation is subtracted from the signal value output from the light detection unit 42, thereby removing the Raman scattered light component. Become. The subtraction results of the signal values output from the light detection units 12, 22, and 32 represent the intensity of fluorescence and chemiluminescence when irradiated with excitation light, and represent the intensity of chemiluminescence when not irradiated with excitation light. The result of subtracting the latter from represents the intensity of fluorescence. In this way, the intensity of each of the fluorescence and chemiluminescence among the light generated in the sample in each of the light detection units 12, 22, 32 can be measured separately and with high accuracy.

  As described above, the stimulating agent is dropped onto the sample (neutrophil-like cell) by the injection units 15, 25, and 35 while performing excitation light irradiation, Raman scattered light component removal, chemiluminescence intensity detection, and fluorescence intensity detection. For example, neutrophil migratory peptide fMLP (formyl-methionyl-leucyl-phenylalanine) is used as a stimulant. Then, the control unit 50 analyzes the causal relationship and temporal relationship between the dropping of the stimulant to the sample, the chemiluminescence intensity (superoxide production amount) and the fluorescence intensity (intracellular calcium concentration) in real time, The analysis result is displayed on the display unit 60 in real time.

FIG. 8 is a graph showing the relationship between the fluorescent indicator (fluorescein) concentration and the detected light intensity. In this graph, the solid line indicates the case of the example in which the Raman scattered light component is removed by the method as described above, and the broken line indicates the case of the comparative example in which the Raman scattered light component is not removed. In the comparative example (broken line), the detected light intensity (fluorescence intensity + Raman scattered light intensity) is not proportional to the concentration of the fluorescent indicator due to the influence of the Raman scattered light. Since the scattered light component is removed, the detected light intensity (fluorescence intensity) is proportional to the fluorescent indicator concentration up to the region of the fluorescent indicator concentration of 10 ⁻¹¹ mol / L. From this, it can be seen that a fluorescent reagent with a lower concentration can be measured with high accuracy by removing the Raman scattered light component using the luminescence measuring apparatus 1 according to the present embodiment.

  Since the luminescence measuring apparatus 1 according to the present embodiment includes the four units 10, 20, 30, and 40, not only can the above-described effects be achieved, but also the following effects can be achieved. Can do. That is, even if the same cell population is used as a sample, the cell activity decreases with the passage of time. It cannot be distinguished whether it is due or not. On the other hand, in this embodiment, the same cell population is stored as sample in the sample cells 19, 29, 39, and the reference sample is stored in the sample cell 49, and these can be measured at the same time. It is very effective for quantification. Further, since both or any one of the fluorescence and chemiluminescence of each of the two types of samples can be measured, it is also effective for elucidating the mutual relationship and action mechanism of these two types of samples.

  Furthermore, the luminescence measuring apparatus 1 according to the present embodiment can be effective even in an assay using a chemiluminescent substance or a chemiluminescent substance. That is, since many chemiluminescent substances are unstable over time, it is difficult to measure a minute difference between a plurality of samples due to a change with time of chemical substances when measurement times are different. On the other hand, since the luminescence measuring apparatus 1 according to the present embodiment includes the four units 10, 20, 30, and 40, a plurality of samples can be measured at the same time. Can be measured.

It is a lineblock diagram of luminescence measuring device 1 concerning this embodiment. It is a lineblock diagram of the 1st unit 10 contained in luminescence measuring device 1 concerning this embodiment. 2 is a perspective view of a first sample cell 19. FIG. 3 is a partial cross-sectional view showing a configuration of a first excitation unit 11. FIG. 3 is a partial cross-sectional view illustrating a configuration of a first detection unit 12. FIG. It is a perspective view of the luminescence measuring device 1 concerning this embodiment. It is a perspective view of the luminescence measuring device 1 concerning this embodiment. It is a graph which shows the relationship between a fluorescent indicator (fluorescein) density | concentration and detected light intensity.

Explanation of symbols

  DESCRIPTION OF SYMBOLS 1 ... Luminescence measuring apparatus, 2 ... Base | substrate, 3 ... Top cover, 10 ... 1st unit, 11 ... 1st excitation part, 12 ... 1st detection part, 13 ... 1st stirring part, 14 ... 1st temperature control part, 15 ... 1st injection part, 19 ... 1st sample cell, 20 ... 2nd unit, 21 ... 2nd excitation part, 22 ... 2nd detection part, 25 ... 2nd injection part, 29 ... 2nd sample cell, 30 ... 1st 3 units, 31... Third excitation unit, 32... Third detection unit, 35... Third injection unit, 39... Third sample cell, 40. 45 ... 4th injection part, 49 ... 4th sample cell, 50 ... control part, 60 ... display part.

Claims (4)

A first excitation unit that irradiates the sample in the first sample cell with excitation light;
A first detector for detecting light generated in the sample in the first sample cell;
A first injection part for injecting a reagent into the first sample cell;
A second excitation unit for irradiating the sample in the second sample cell with excitation light;
A second detector for detecting light generated in the sample in the second sample cell;
A second injection part for injecting a reagent into the second sample cell;
A third excitation unit that irradiates the sample in the third sample cell with excitation light;
A third detector for detecting light generated in the sample in the third sample cell;
A third injection part for injecting a reagent into the third sample cell;
A fourth excitation unit for irradiating the sample in the fourth sample cell with excitation light;
A fourth detector for detecting light generated in the sample in the fourth sample cell;
A fourth injection part for injecting a reagent into the fourth sample cell;
A control unit that performs control so that any two or more of the first detection unit, the second detection unit, the third detection unit, and the fourth detection unit detect light simultaneously;
With
The optical systems of the first excitation unit, the second excitation unit, the third excitation unit, and the fourth excitation unit have the same configuration,
The optical systems of the first detection unit, the second detection unit, the third detection unit, and the fourth detection unit have the same configuration,
The first excitation unit, the second excitation unit, the third excitation unit, the fourth excitation unit, the first detection unit, the second detection unit, the third detection unit, and the fourth detection unit, Arranged around the first sample cell, the second sample cell, the third sample cell and the fourth sample cell;
In the first sample cell, the optical axes of the optical systems of the first excitation unit and the first detection unit are orthogonal to each other,
In the second sample cell, the optical axes of the optical systems of the second excitation unit and the second detection unit are orthogonal to each other,
In the third sample cell, optical axes of the optical systems of the third excitation unit and the third detection unit are orthogonal to each other,
In the fourth sample cell, the optical axes of the optical systems of the fourth excitation unit and the fourth detection unit are orthogonal to each other,
Optics of the first excitation unit, the second excitation unit, the third excitation unit, the fourth excitation unit, the first detection unit, the second detection unit, the third detection unit, and the fourth detection unit, respectively. The optical axis of the system is parallel to either the first direction or the second direction orthogonal to each other;
A luminescence measuring device characterized by that.   A first stirring unit for stirring the sample in the first sample cell; a second stirring unit for stirring the sample in the second sample cell; and a third stirring unit for stirring the sample in the third sample cell; The luminescence measurement apparatus according to claim 1, further comprising a fourth stirring unit that stirs the sample in the fourth sample cell.   The display device according to claim 1, further comprising a display unit configured to display in real time the status of light detection performed by each of the first detection unit, the second detection unit, the third detection unit, and the fourth detection unit. Luminescence measuring device.   The control unit detects light from one of the first detection unit, the second detection unit, the third detection unit, and the fourth detection unit based on a light detection result by any one of the detection units. The luminescence measuring apparatus according to claim 1, wherein the result is subtracted.

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