JP4417860B2 - Cell culture equipment - Google Patents

Description

  The present invention relates to a cell culture facility for culturing cells, and more particularly to a cell culture facility in which a cell culture apparatus is arranged in a clean room.

  Conventionally, in order to cultivate cultures such as various microorganisms and cells, a culture apparatus (incubator) that accommodates the cultures and adjusts the culture environment has been used. In particular, tissue culture of mammalian cells such as human cells has recently been actively performed using such a culture apparatus. For example, fertilized eggs and cardiomyocytes are cultured.

  In order to prevent contamination of bacteria, dust, etc. from the atmosphere into the culture object, such a culture apparatus generally has a class of 100,000 (100,000 dust of 0.5 μm diameter contained in one cubic foot). It is necessary to install it in the clean space of the following), and it is placed in a clean room managed in class 100,000.

And this clean room generally increases the cleanliness of the clean room by discharging the air flow from the ceiling through the air filter in the floor direction and sucking this air flow from the suction port provided on the inner wall near the floor. (For example, see Non-Patent Document 1).
"Nikkei Biobusiness March 2003", Nikkei Business Publications, February 2003, p60-71

  However, in the conventional clean room, the box-shaped culture apparatus is placed in the room as it is, so that the room space has to be widened. In addition, the culture apparatus changed the flow of airflow for increasing the cleanliness, and the cleanliness in the room was low.

  Furthermore, depending on the place where this culture apparatus is installed, the place where the culture apparatus is installed is limited because the suction port for sucking an air flow for increasing the cleanliness is blocked.

  In addition, since the inspection and repair of the culture apparatus had to be performed in a clean room, the work could not be easily performed, and at that time, the cleanliness in the clean room could be affected.

Therefore, in the present invention according to claim 1, the cell culture apparatus is disposed in a clean room in which air is blown from the ceiling via an air filter and the air is sucked from an air inlet provided on the floor or an inner wall near the floor. a cell culture facilities, the arrangement of the cell culture apparatus of the clean room, both when the protruded main body portion of the cell culture apparatus from the inner wall surface of the clean room external side, the inner and the cultures outlet portion of a cell culture apparatus It is characterized by being substantially flush with the wall surface.

  Moreover, in this invention which concerns on Claim 2, in the cell culture equipment of Claim 1, the to-be-cultured object operation stand for test | inspecting to-be-cultured object is equipped with the 2nd inner wall surface facing a cell culture apparatus, It is characterized in that the front end face portion is located on substantially the same plane.

  Moreover, in this invention which concerns on Claim 3, in the cell culture equipment of Claim 1 or Claim 2, the door which covers the to-be-cultured material taking-out part of a cell culture apparatus, and a door in the direction parallel to an inner wall surface A slide mechanism for sliding is provided.

  Moreover, in this invention which concerns on Claim 4, in the cell culture equipment of Claim 1 or Claim 2, a cell culture apparatus is a substantially box-shaped tray which opened the upper part, and for accommodating a tray inside A cell culture chamber having an opening, and the front portion of the tray is exposed from the opening in a state where the tray is accommodated in the cell culture chamber.

  The invention according to claim 5 is characterized in that, in the cell culture facility according to claim 4, a heater is provided on a front portion of the tray.

  Further, in the present invention according to claim 6, in the cell culture facility according to claim 4 or 5, the tray has at least a plurality of vent holes formed on the bottom surface thereof.

  Moreover, in this invention which concerns on Claim 7, in the cell culture equipment of any one of Claims 4-6, in the state which accommodated the several tray in the cell culture room, a shelf part is provided between trays. The shelf is provided with an antenna for wirelessly communicating with the IC tag.

  Further, in the present invention according to claim 8, in the cell culture facility according to claim 7, the cell culture device is placed on a tray, accommodates a culture object, and is attached with an IC tag. And a wireless unit that communicates with the IC tag via the antenna, and a control unit that detects information of the IC tag acquired by the wireless unit and manages the culture object based on the detection result. It is characterized by that.

  Further, in the present invention according to claim 9, in the cell culture facility according to claim 8, the cell culture apparatus includes a communication interface that communicates with an external computer and a communication interface that is transmitted from the external computer via the interface. The storage device for storing the information of the container take-out procedure, the lock mechanism for locking the tray in the storage state to be unremovable, and the container take-out are performed in a procedure different from the information of the container take-out procedure stored in the storage means. When this is detected, the lock mechanism is operated to lock the container so that it cannot be taken out.

  Further, in the present invention according to claim 10, in the cell culture facility according to claim 9, the cell culture apparatus has alarm means for emitting an alarm sound or an alarm light, and the control means is stored in the storage means. When it is detected that the container has been removed in a procedure different from the information on the container removal procedure, an alarm sound or warning light is emitted by the alarm means.

  And in this invention, there exists an effect described below.

That is, according to the present invention of claim 1, the cell culture apparatus is provided in a clean room in which air is blown from the ceiling via the air filter and the air is sucked from an air inlet provided on the floor or an inner wall near the floor. a placement cell culture facilities, the arrangement of the cell culture apparatus of the clean room, both when the protruded main body portion of the cell culture apparatus from the inner wall surface of the clean room external side, the cultures outlet portion of a cell culture apparatus The air flow channel that is blown into the clean room from the ceiling through the air filter is not obstructed by the cell culture device, and the air intake provided on the floor or the inner wall near the floor. It will be sent to the intake duct through the mouth, and it becomes easy to maintain cleanliness in the clean room.

  According to the second aspect of the present invention, the culture object operation table for inspecting the culture object is provided on the substantially same surface as the second inner wall surface facing the cell culture device, and its front end surface. Since the part is arranged so that the part is located, the flow path of air sent from the air filter into the clean room is sent to the intake duct without being disturbed by the culture object operation table, and the cleanliness in the clean room It becomes easy to keep.

  Further, according to the present invention according to claim 3, since the door that covers the part to be cultured to be taken out of the cell culture device and the slide mechanism that slides the door in a direction parallel to the inner wall surface are provided, The door does not get in the way when working.

  According to the fourth aspect of the present invention, the cell culture device includes a substantially box-shaped tray having an upper opening, and a cell culture chamber having an opening for accommodating the tray inside. Since the front part of the tray is exposed from the opening while being accommodated in the cell culture chamber, it can be grasped at a glance whether or not the tray is accommodated in the cell culture chamber.

  According to the fifth aspect of the present invention, since the heater is provided on the front portion of the tray, the temperature in the vicinity of the opening, which is difficult to maintain the temperature, can be kept constant, and is generated due to variations in temperature. Problems such as condensation can be avoided.

  Further, according to the present invention of claim 6, since the tray has a plurality of vent holes formed at least on the bottom surface thereof, the entire cell culture chamber can be ventilated, and the temperature, humidity and Co2 concentration in the tray can be obtained. Is kept constant.

  According to the seventh aspect of the present invention, the cell culture chamber is provided with a shelf between the trays in a state where a plurality of trays are accommodated, and the shelf communicates wirelessly with the IC tag. Therefore, even when the tray or shelf plate is made of a metal plate having a plurality of holes, it is possible to reliably communicate with the IC tag of the container 25.

  According to the present invention of claim 8, the cell culture device is placed on the tray, accommodates the culture object, and has an IC tag attached to the container, the IC tag and the antenna. Since a wireless unit for communication and information on the IC tag acquired by the wireless unit are detected, and a control unit for managing the culture object is provided based on the detection result, work mistakes by the operator are prevented. It becomes possible.

  According to the present invention of claim 9, the cell culture device stores a communication interface that communicates with an external computer, and information that stores information on a container removal procedure transmitted from the external computer via the interface. And a lock mechanism that locks the tray in the storage state so that it cannot be removed, and the lock mechanism is detected when it is detected that the container has been removed in a procedure different from the information of the container removal procedure stored in the storage means. Since the container is locked so that the container cannot be taken out, the operator can easily understand that there is an error in the work procedure, and work errors can be reduced.

  Further, according to the present invention of claim 10, the cell culture device has an alarm means for emitting an alarm sound or an alarm light, and the control means is a procedure different from the information on the container removal procedure stored in the storage means. When it is detected that the container has been taken out, an alarm sound or warning light is emitted by the alarm means, so that the operator can easily understand that there is an error in the work procedure and reduce work errors. It becomes possible to do.

  Hereinafter, the culture equipment in the embodiment of the present invention will be specifically described with reference to the drawings.

  1 is a perspective view of a cell culture facility according to an embodiment of the present invention, FIG. 2 is a plan sectional view of the cell culture facility according to an embodiment of the present invention, and FIG. 3 is a transverse sectional view of the cell culture facility according to an embodiment of the present invention. is there.

  As shown in FIGS. 1 and 2, the cell culture facility according to the embodiment of the present invention includes a clean room 1 having a class 1 million cleanliness, and cell culture chambers 21 a and 21 b for culturing a culture object such as a fertilized egg. The cell culture apparatus 2a, 2b and the culture object operation table of the present invention used for taking out the culture object accommodated in the cell culture apparatus 2a, 2b and inspecting the culture object, etc. Adjacent to the cabinet 3 and the clean room 1 and an air shower room 4 for removing germs and dust adhering to the worker in a short time, and changing clothes for the operator to change clothes next to the air shower room 4 It is comprised from the chamber 5 and the air conditioner 6 etc. for maintaining the cleanliness of the clean room 1 or the air shower chamber 4.

  And the doors 7a-7c are provided between the cell culture equipment exterior and the changing room 5, between the air shower room 4 and the changing room 5, and between the clean room 1 and the air shower room 4, respectively. An operator can enter and exit the clean room 1 through the changing room 5 and the air shower room 4 from outside the cell culture facility.

  In addition, this cell culture facility is provided with air supply ducts 8a to 8f which are air passages sent from the air conditioning device 6 to the clean room 1, the air shower room 4, and the changing room 5, and thus sent. Air is sent to each room through air filters 9a to 9c provided on the roof top of each room. And the air sent to each room in this way is inhaled by the air conditioner 6 through the intake ducts 10a to 10d provided in the room.

  Moreover, as shown in FIG. 3, the air sent from the air conditioner 6 is sent into the clean room 1 through the air filter 9c from the air supply duct 8f provided in the upper part of the roof of the clean room 1.

  The air sent to the clean room 1 is sucked from a plurality of air inlets 12a to 12d provided near the floor of the inner wall surface 11a and an air inlet 12e provided near the floor on the rear surface of the safety cabinet 3. The intake ports 12a to 12e may be arranged on the floor portion.

  Thus, in order to maintain the cleanliness of the clean room 1, the air inlet of the clean room 1 as a whole becomes easy because the air inlet is provided on the ceiling and the air outlet is provided on or near the floor. In addition, accumulation of dust and the like near the floor can be prevented.

  In the cell culture devices 2a and 2b, the main body portions 13a and 13b project outward from the inner wall surface 11a, and the culture object take-out port portions 14a and 14b are substantially flush with the inner wall surface 11a. .

  Therefore, the flow path of the air blown into the clean room 1 from the ceiling through the air filter 9c is not obstructed by the cell culture device 2, but through the air inlets 12a to 12e provided in the inner wall near the floor. Therefore, the cleanliness in the clean room 1 can be easily maintained.

  Furthermore, since the culture object take-out port portions 14a and 14b are substantially flush with the inner wall surface 11a, the operator can easily take out the culture object.

  Moreover, since it is not necessary to arrange the cell culture device 2 in the clean room 1, the area of the clean room 1 can be reduced, and thus the driving ability of the air conditioning device 6 can be reduced.

  Moreover, as shown in FIG. 2, the main body portions 13a and 13b of the cell culture device 2 are outside the clean room so that the mechanism and control portions of the cell culture device 2 can be inspected and repaired from outside the cell culture equipment. Therefore, the inspection and repair work in the clean room 1 is not necessary, and it is possible to avoid complications such as wearing work clothes for the clean room, and the work becomes extremely easy. .

  Here, the main body portions 13a and 13b of the cell culture device 2 are composed of at least the cell culture chambers 21a and 21b of the cell culture device 2 and their control portions.

  On the other hand, the safety cabinet 3 is arranged on the substantially same surface of the inner wall surface 11b facing the inner wall surface 11a so that the front end surface portion 31d is located. And the safety cabinet 3 is arrange | positioned in the position facing the cell culture apparatus 2 so that the main-body part may protrude outside the inner wall surface 11b. The main body 31 of the safety cabinet 3 includes at least an operation table 31a for operating the culture object. In the present embodiment, the operation table 31a that operates at a substantially central portion in the height direction and operates the culture object, and the intake that is positioned in the back of the operation table and sucks air to increase the cleanliness of the operation table. A mouth 31b, an air intake duct 10b for sending air sucked from the air inlet to the air conditioner 6, and a worker foot insertion space 31c, which is located at the lower part of the operation table and into which an operator's foot can be put. Etc.

  Therefore, the flow path of the air sent from the air filter 9c into the clean room 1 is sent to the intake duct without being obstructed by the safety cabinet 3, and it becomes easy to maintain the cleanliness in the clean room 1. .

  Moreover, since it is not necessary to arrange the safety cabinet 3 in the clean room 1, the area of the clean room 1 can be reduced, and thus the driving ability of the air conditioner 6 can be reduced.

  In addition, since the safety cabinet 3 is arranged at a position facing the cell culture device 2, the specimen of the culture object can be easily obtained. That is, since the distance between the cell culture device 2 and the safety cabinet 3 is close, the distance to carry the culture object can be shortened and the operation can be facilitated. Moreover, since the distance is short, when carrying a to-be-cultured object, the risk of spilling the contents can be reduced.

  Next, the configuration of the cell culture device 2 will be specifically described with reference to the drawings.

  FIG. 4 is a front view of the first cell culture device of the embodiment of the present invention.

  As shown in FIG. 4, the cell culture apparatus 2 covers the culture object take-out port portions 14a and 14b with slide doors 20a and 20b that are openable and slidable. By slid 20a and 20b upward and locking at a predetermined position, the culture object take-out port portions 14a and 14b are exposed. The slide doors 20a and 20b are disposed on the clean room 1 side with the inner surfaces substantially the same as the inner wall surface 11a.

  Thus, since the slide doors 20a and 20b that can slide up and down are used, there is no need to increase the width between the cell culture apparatus and the safety cabinet. Note that the sliding direction may be the left-right direction as long as it can slide in a direction parallel to the inner wall surface 11b.

  That is, in the case of a hinged door, the size must be designed in consideration of the width between the cell culture device 2 and the safety cabinet 3, which is complicated. In addition, when the door is opened, the work may be disturbed.

  However, since the sliding door is adopted, there is no such drawback.

  The mechanism for sliding the slide doors 20a, 20b is arranged as slide rails on the left and right sides of the culture object take-out port portions 14a, 14b, and the slide doors 20a, 20b are lifted up to a predetermined position. When the slide doors 20a and 20b are engaged with the slide rails and the slide doors are locked, the slide door is locked. The lock is released by pulling the slide doors 20a and 20b downward with a predetermined force. Adopted mechanism.

  In addition, the slide doors 20a and 20b are provided with two recesses at the lower end portion for inserting a finger on the outer surface (clean room side), so that the user can easily raise and lower the slide doors 20a and 20b. .

  A heater is attached to the entire inner surface of the slide doors 20a and 20b, that is, the surface facing the culture-subject take-out port portions 14a and 14b in order to alleviate temperature variations in the cell culture chambers 21a and 21b. It has been. The heat from the heater is uniformly released into the cell culture chambers 21a and 21b by the heat diffusion member attached to the inner surfaces of the slide doors 20a and 20b.

  The above has described the structure in which the cell culture chambers 21a and 21b are covered with the slide door 20, but the cell culture devices 2a and 2b that do not require such doors in the cell culture chambers 21a and 21b will be described below with reference to the drawings. Will be described in detail.

  FIG. 5 is a front view of the second cell culture device according to the embodiment of the present invention, and FIG. 6 is a view showing a state in which the tray is pulled out from the second cell culture device according to the embodiment of the present invention.

  As shown in FIG. 5, the front panels 221aa to 221aw and 221ba to 221bw of the plurality of trays 22aa to 22aw and 22ba to 22bw are exposed, and the front panels 221aa to 221aw and 221ba to 221bw correspond to the cell culture chamber 21a. It functions as a substitute for the door of 21b.

  And as shown in FIG. 6, 14a and 14b are formed in the to-be-cultured material taking-out opening 23aa-23aw and 23ba-23bw opened in the substantially rectangular shape in order to accommodate each tray 22aa-22aw and 22ba-22bw The tray take-out panels 24a and 24b are arranged on substantially the same plane as the inner wall surface 11a, and the front panels 221aa to 221aw and 221ba to 221bw can cover the openings 23aa to 23aw and 23ba to 23bw. Further, it is formed from a flat plate having an area larger than the substantially rectangular area of the openings 23aa to 23aw and 23ba to 23bw.

  It should be noted that alphabets may be omitted from the reference numerals to which alphabets are added, and in this case, any one or all of those represented by alphabets. For example, the tray 22 refers to one or all of the trays 22aa to 22aw and 22ba to 22bw, and the opening 23 refers to any one or all of the openings 23aa to 23aw and 23ba to 23bw. Shall.

  In FIG. 6, only the tray 22bf, the opening 23bf, the tray take-out panel 24b, the front panel 221bf, the heater 222bf, the temperature sensor 223bf, the metal flat plates 224bf to 227bf, the connection terminals 228bf, the container 25bf, etc. are shown by reference numerals. Other symbols are omitted.

  As described above, the cell culture device 2 is configured, and the front panel 221 of each tray 22 is in close contact with the surface of the tray take-out panel 24, and the front panel 221 closes the opening 23, so that the inside of the cell culture chamber 21 And the inside of the clean room 1 are blocked, and the temperature, humidity, and Co2 concentration in the cell culture chamber 21 are kept constant. The tray 22 is provided with a container 25 such as a petri dish in which a culture object is stored.

  Further, as shown in FIG. 6, each tray 22 is formed in a substantially box shape with an upper portion as an opening, and except for the front panel 221, metal flat plates 224 to 239 such as an aluminum plate having a mesh-shaped small-diameter hole are provided. 227. The entire diameter of the cell culture chamber can be ventilated by the small-diameter holes of the metal flat plates 224 to 227, and the temperature, humidity and Co2 concentration in the tray 22 are kept constant. That is, the small-diameter holes of the metal flat plates 224 to 227 are made to function as vent holes.

  Note that this vent hole only needs to be formed in at least the metal flat plate 227, but by forming it in the metal flat plates 224 to 226, the ventilation in the cell culture chamber 21 is further improved, and the temperature and humidity inside the cell culture chamber 21 are improved. In addition, the Co2 concentration can be kept uniform.

  Here, a heater 222 bent into a continuous S-shape inside or inside (front side of the cell culture chamber) of each tray 22 so that heat is uniformly released from the entire front panel 221. Is arranged.

  Further, the front panel 221 is provided with a temperature sensor 223 for measuring the temperature of the heater 222, and the heat released by the heater 222 is made constant based on the temperature detected by the temperature sensor 223. Can do.

  Therefore, the temperature in the vicinity of the opening 23, which is difficult to maintain the temperature, can be kept constant, and problems such as condensation caused by temperature variations can be avoided.

  Further, a connection terminal 228 for connecting the heater 222 and the temperature sensor 223 to the main body of the cell culture device 2 is provided at the rear end of the tray 22, and the tray 22 is accommodated in the cell culture chamber 21. The connection terminal 228 is connected to the control unit of the cell culture apparatus 2 main body.

  Further, the front panel 221 of the tray 22 has a convex portion 229 projecting in a substantially vertical direction on the inner wall surface 11a, and the user can pull out and store the tray 22 using the convex portion 229. is there.

  FIG. 7 is a cross-sectional view showing a state in which the tray of the second cell culture device of the embodiment of the present invention is housed. As shown in this figure, the tray 22 is taken out into each opening 23. A shielding plate 26 is provided for shielding the cell culture chamber 21 and the clean room 1 from each other.

  One end of the shielding plate 26 is pivotally connected to the back side of the upper tray take-out panel 24 of the opening 23, and the other end is an open end.

  When the tray 22 is removed, the other end of the shielding plate 26 comes into contact with the back side of the tray take-out panel 24 below the opening 23 due to its gravity.

  Further, when the tray 22 is inserted into the opening 23, the other end of the shielding plate 26 is lifted upward by the rear end plate 224 of the tray 22, and then slides on top of the left and right end plates 225, 226 of the tray. Stop at position. FIG. 7 shows this state.

  Since such a shielding plate 26 is provided, even when the tray 22 is pulled out, it is possible to minimize the change in the state of the cell culture chamber, and the heater 222 on the front panel 221 of the tray 22 is combined. Thus, it is possible to dispense with a door for taking out the culture object.

  As shown in FIG. 6, the tray take-out panel 24 has a lamp 15 attached to the side surface of the opening 23 and is controlled by the control unit 18 described later.

  As shown in FIG. 7, a plurality of shelf plates 27, which are the shelf portions of the present invention, are provided in parallel with the trays 22 between the upper and lower trays 22 when the trays 22 are accommodated.

  Similar to the tray 22, the shelf plate 27 is formed of a metal flat plate such as an aluminum plate having a mesh-shaped small-diameter hole. The small-diameter hole of the metal flat plate allows the entire cell culture chamber 21 to be ventilated. The temperature, humidity and Co2 concentration in the tray 22 are kept constant.

  The shelf plate 27 is provided with an antenna 28 for communicating with an IC tag (wireless tag) attached to the container 25. The antenna 28 is connected to a wireless unit 19 described below. ing.

  As described above, since the antenna 28 is attached to each shelf 27, even when the tray 22 or the shelf 27 is made of a metal plate having a plurality of holes, it reliably communicates with the IC tag of the container 25. can do.

  In addition, since each antenna 28 has a wireless signal having a strength that can communicate with only one culture vessel, the culture vessel is placed on each tray 22 depending on whether a control unit described later can communicate with a tag attached to the culture vessel. It can be determined whether or not.

  Hereinafter, a control block diagram of the cell culture apparatus will be specifically described with reference to the drawings.

  FIG. 8 is a diagram showing a control block diagram of the cell culture device according to the embodiment of the present invention, and FIG. 9 is a diagram showing connection points with each tray according to the embodiment of the present invention.

  As shown in FIG. 8, the cell culture apparatus 2 includes a gas supply unit 30 that supplies Co2 gas having a predetermined concentration (for example, 5%), and an inflow amount of gas from the gas supply unit 30 to the cell culture chamber 21. An inflow amount adjusting unit 39 for adjusting the volume, a control panel 32 having a display unit and an input unit, an antenna 28 attached to each shelf plate 27, and a lamp provided on the tray take-out panel 24 so as to correspond to the tray 22 15, a communication interface 16 for communicating with an external computer 34, a storage unit 17 for storing control contents and various setting contents, a control unit 18 for controlling the entire cell culture apparatus, and an antenna 28, connected to the cell A wireless unit 19 for communicating with the IC tag in the culture chamber 21, a main body heater 35 for heating the cell culture chamber 21, a main body temperature sensor 36 for measuring the temperature in the cell culture chamber 21, and the cell culture chamber A humidity sensor 37 etc. is provided to measure the humidity in 21. It has been.

  Further, as shown in FIG. 9, each tray 22 is provided with a connection terminal 228, and a heater power supply line 40 for supplying power for heating the heater attached to the front panel 221 of the tray 22, A tray temperature sensor signal line 41 for detecting the temperature of the cell culture chamber 21 and a tray accommodation detection line 42 for detecting the accommodation of the tray 22 are provided.

  The control unit 18 detects the temperature from the tray temperature sensor signal line 41, and supplies the power to the heater 222 in the container so that the temperature of the front panel of the tray becomes the set temperature. Control the power supplied.

  In this way, the inside of the cell culture chamber 21 can be heated evenly from the tray front panel 221 by the heater 222 and from the upper part of the cell culture chamber 21 by the main body heater 35, so that condensation occurs on the front panel 221 of the tray. Can be easily prevented.

  The operation relating to the tag of the cell culture device 2 configured as described above will be described in detail with reference to the drawings.

  FIG. 10 is a flowchart showing an operation related to the IC tag of the cell culture device 2.

  First, when an operator inputs an operation procedure of the cell culture apparatus 2 from the external computer 34 via the communication interface 16 (step S1), information on the operation procedure is stored in the storage unit 17 by the control unit 18 ( Step S2).

  Here, the operation procedure is a container take-out procedure of how and when to take out the cultures contained in each container 25 in what order and how, for example, the container 25c is removed on the morning of January 10 After taking out the sample from the cell culture device 2 at 9 o'clock and returning the container 25c to the cell culture device 2 and taking out the sample from the container 25d at 2:00 pm on January 10th, the container 25d is taken into the cell culture device. It is a procedure such as returning to 2.

  Next, the control unit 18 determines whether or not the time required to operate the container 25 has arrived based on the operation procedure information stored in the storage unit 17 (step S3).

  When it is determined that the time required to operate the container 25 has arrived, the tray 22 in which the container 25 that needs to be operated is stored is analyzed. This analysis is performed based on the tag information of the container 25 stored in the storage unit 17 together with the operation procedure information.

  That is, the communication range of the antenna 28 provided on the shelf board 27 is determined by the radio signal having a strength such that only the IC tag of the enclosure 25 placed on the tray 22 above the shelf board 27 is used. It can be determined whether the tray 22 is placed.

  Then, the lamp 15 corresponding to the tray 22 on which the container 25 is placed blinks (step S4).

  Thus, since the lamp 15 corresponding to the tray 22 on which the container 25 is placed blinks, the operator can easily take out the tray 22 on which the target container 25 is placed.

  When the control unit 18 determines that the tray 22 has been removed from the cell culture chamber 21 or stored by the operator (step S5), the control unit 18 operates the operation procedure stored in the storage unit 17. It is determined whether or not the information matches (step S6).

  For example, when the tray 22 is pulled out, if the tray is a tray 22 different from the tray 22 corresponding to the blinking lamp 15, it is determined that the operation procedure information does not match.

  If the control unit 18 determines that the operation procedure is not followed (step S6: Y), the control unit 18 locks the cell culture device 2 (step S8). This locking is performed by a locking mechanism between each tray 22 and the cell culture chamber 21. That is, the control unit 18 drives a locking portion (not shown) provided in the vicinity of the inside of the tray take-out panel 24 and engages with a locking portion (not shown) of each tray 22 to perform locking.

  As described above, when the lock mechanism is operated by the control unit 18, the tray 22 accommodated in the cell culture chamber 21 cannot be taken out.

  However, the tray 22 taken out from the cell culture chamber 21 does not have to be locked in the locking mechanism corresponding to the extracted opening 23. In this case, the tray 22 taken out is not in the cell culture chamber 21. Can be accommodated.

  In addition to this locking mechanism, or in addition to this locking mechanism, an alarm means (not shown) that emits an alarm sound or warning light is provided, and an operation procedure in which the operation of the tray 22 is stored in the storage unit 17 by the control unit 18 When it is determined whether or not it matches the information, an alarm sound or / and an alarm light may be emitted by the alarm means. Further, this can be displayed on the control panel 32.

  In this way, the operator can easily understand that there is an error in the work procedure, and the work mistake can be reduced.

  On the other hand, when the control unit 18 determines that the operation procedure is being followed (step S6: N), the control unit 18 determines whether all the operation procedure information stored in the storage unit 17 has been completed (step S7).

  And when all the operation procedure information memorize | stored in the memory | storage part 17 is complete | finished (step S7: Y), the control part 18 complete | finishes these operation | movement.

  If all the operation procedure information stored in the storage unit 17 has not been completed (step S7: N), it is determined whether or not the lamp 15 needs to blink (step S9), and the lamp 15 needs to blink. If there is (step S9: Y), the lamp 15 blinks (step S10).

  For example, when the operator takes out the tray 22 corresponding to the lamp 15 according to the blinking of the lamp 15, the control unit 18 indicates that the drawer 22 is stored according to the operation procedure information stored in the storage unit 17. In order to notify the worker, the lamp 15 is continuously blinked.

  When the operator stores the pulled out tray 22, the control unit 18 ends the blinking of the lamp 15.

  In the present embodiment, the communication range of the antenna 28 provided on the shelf board 27 is accommodated by a radio signal having a strength such that only the tag of the container 25 placed on the tray 22 above the shelf board 27 is accommodated. Although the container 25 and the tray 22 are associated with each other, the container 25 and the tray 22 may be associated with each other by other methods.

  For example, each time the tray 22 is pulled out or accommodated in the cell culture chamber 21 by the control unit 18, the relationship between the IC tag of the container 25 that can communicate with each antenna 28 and the tray 22 is confirmed. The storage unit 17 stores the association of which container 25 is placed in the storage unit 17.

  In this way, each time the container 25 is accommodated, it can be determined which tray 22 the container 25 is placed on, and the information is stored in the storage unit 17. 18 can grasp on which tray 22 the container 25 is placed.

  As described above, since the IC tag is detected, when a plurality of containers 25 are accommodated in the cell culture apparatus 2, the problem that the containers 25 are mistaken in the past has been solved. It becomes possible to do. In addition, it is very easy to take out the container 25 from the cell culture device 2 and to record and manage the container.

It is a perspective view of the cell culture equipment of an embodiment of the present invention. It is a plane sectional view of the cell culture equipment of an embodiment of the present invention. It is a cross-sectional view of the cell culture facility of the embodiment of the present invention. It is a front view of the 1st cell culture equipment of the embodiment of the present invention. It is a front view of the 2nd cell culture device of an embodiment of the present invention. It is a perspective view which shows the state which pulled out the tray from the 2nd cell culture apparatus of embodiment of this invention. It is a cross-sectional view which shows the state which accommodated the tray of the 2nd cell culture apparatus of embodiment of this invention. It is a figure which shows the control block diagram of the cell culture apparatus of embodiment of this invention. It is a figure which shows the connection location with each tray of embodiment of this invention. It is a flowchart which shows the operation | movement regarding the tag of the cell culture apparatus of embodiment of this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Clean room 2 Cell culture apparatus 3 Safety cabinet 6 Air conditioning apparatus 11 Inner wall surface 13 Cell culture apparatus main-body part 14 To-be-cultured material taking-out part 19 Radio | wireless part 20 Slide door 21 Cell culture room 22 Tray 25 Container 27 Shelf board 28 Antenna 29 Radio section

Claims (10)

A cell culture facility in which a cell culture device is arranged in a clean room in which air is blown from the ceiling via an air filter and the air is sucked from an air inlet provided on the floor or an inner wall near the floor,
The arrangement of the cell culture apparatus to the clean room, the protruded main body portion of the cell culture apparatus to the external side of the inner wall surface of the clean room together, and the object to be culture outlet portion of the cell culture apparatus the inner wall surface A cell culture facility characterized by having substantially the same surface. The cell culture facility according to claim 1,
A cell culture facility in which a culture object operation table for inspecting a culture object is disposed so that a front end surface portion thereof is positioned on substantially the same surface as a second inner wall surface facing the cell culture device. The cell culture facility according to claim 1 or 2,
A door that covers a portion of the cell culture device to be cultured,
A cell culture facility comprising a sliding mechanism for sliding the door in a direction parallel to the inner wall surface. The cell culture facility according to claim 1 or 2,
The cell culture device comprises:
A substantially box-shaped tray having an upper opening, and a cell culture chamber having an opening for accommodating the tray inside,
A cell culture facility characterized in that the front portion of the tray is exposed from the opening while the tray is accommodated in the cell culture chamber. The cell culture facility according to claim 4,
A cell culture facility, wherein a heater is provided on a front portion of the tray. The cell culture facility according to claim 4 or 5, wherein
The cell culture facility, wherein the tray has a plurality of vent holes formed at least on a bottom surface thereof. The cell culture facility according to any one of claims 4 to 6,
In the cell culture chamber, in a state where a plurality of the trays are accommodated, a shelf is provided between the trays,
A cell culture facility, wherein the shelf is provided with an antenna for wirelessly communicating with an IC tag. The cell culture facility according to claim 7,
The cell culture device comprises:
A container that is placed on the tray and that contains the object to be cultured and has an IC tag attached thereto,
A wireless unit that communicates with the IC tag via the antenna;
A cell culture facility, comprising: control means for detecting information on the IC tag acquired by the wireless unit and managing the culture object based on the detection result. The cell culture facility according to claim 8, wherein
The cell culture device comprises:
A communication interface for communicating with an external computer;
Storage means for storing information on a container removal procedure transmitted from the external computer via the interface;
A locking mechanism that locks the tray in a retracted state so that it cannot be removed;
When it is detected that the container has been removed in a procedure different from the container removal procedure information stored in the storage means, the lock mechanism is operated to lock the container so that it cannot be removed. A cell culture facility. The cell culture facility according to claim 9, wherein
The cell culture device comprises:
Having an alarm means for emitting an alarm sound or an alarm light,
The control means emits an alarm sound or a warning light by the alarm means when detecting that the container has been taken out in a procedure different from the information of the container take-out procedure stored in the storage means. Cell culture equipment.

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