JP4350007B2 - Hair restorer - Google Patents

Description

  The present invention relates to a hair growth / hair nourishing agent for the purpose of promoting hair growth and preventing hair loss.

Conventionally, what contains a proanthocyanidin as an active ingredient is known as this kind of hair growth and hair nourishing agent (refer patent document 1). Patent Document 1 has a description that herbal extracts such as assembly extract, garlic extract, carrot extract and aloe extract may be blended as optional components.
International Publication No. 96/000561 Pamphlet

  However, since the hair growth / hair nourishing agent of Patent Document 1 contains only proanthocyanidins as an active ingredient, there is a problem that it is difficult to obtain further hair growth / hair growth effects. Furthermore, Patent Document 1 does not describe the effect when a herbal extract is blended as an optional component, and such knowledge is not obtained at all.

  The present invention has been made paying attention to the problems existing in the prior art as described above. The object is to provide a hair-restoring / hair-restoring agent that can enhance the hair-restoring / hair-restoring effect by exerting a synergistic effect.

In order to achieve the above object, the hair-restoring / hair-growing agent of the invention described in claim 1 comprises (A) proanthocyanidins and (B) aloe extract and (C) ginseng extract as active ingredients, proanthocyanidins (a) contains 0.05 to 2 wt%, (B) the aloe extract contains 0.0001 wt% dry mass basis, the (C) ginseng extract dried The content is 0.0001 to 0.1% by mass based on the mass.

  According to the present invention, it is possible to enhance the hair growth / hair restoration effect by exerting a synergistic effect.

DESCRIPTION OF EMBODIMENTS Hereinafter, embodiments embodying the present invention will be described in detail.
The hair growth and hair nourishing agent of this embodiment contains (A) proanthocyanidins and (B) aloe extract as active ingredients. (A) Proanthocyanidins are contained in an amount of 0.01 to 10% by mass. (B) Aloe extract contains 0.0001-0.1 mass% on the basis of dry mass.

  (A) Proanthocyanidins are contained as an active ingredient that exhibits a hair-growth / hair-restoring effect. Proanthocyanidin refers to a compound obtained by polymerizing a flavan-7-ol derivative represented by the following general formula (1).

（式中、Ｒ¹及びＲ²はそれぞれ独立に水素原子、水酸基、又は−ｏ−ガロイル基を示し、Ｒ³、Ｒ⁴、Ｒ⁵、Ｒ⁶及びＲ⁷はそれぞれ独立に水素原子又は水酸基を示す。）
上記一般式（１）で示されるフラバン−７−オール誘導体の具体例としては、カテキン、エピカテキン、ガロカテキン、エピガロカテキン、アフゼレチン、エピアフゼレチン等が挙げられる。

(In the formula, R ¹ and R ² each independently represent a hydrogen atom, a hydroxyl group, or —o-galloyl group, and R ³ , R ⁴ , R ⁵ , R ^6, and R ⁷ each independently represent a hydrogen atom or a hydroxyl group. Show.)
Specific examples of the flavan-7-ol derivative represented by the general formula (1) include catechin, epicatechin, gallocatechin, epigallocatechin, afuzeletin, and epiafzeletin.

  Proanthocyanidin is a dimer or more of the flavan-7-ol derivative represented by the above general formula (1), preferably 2 to 10 mer, more preferably 2 to 5 mer, further preferably 2 to 3. It is a mer. Proanthocyanidins exceeding the 10-mer of the flavan-7-ol derivative may make it difficult to sufficiently exert the hair-restoring / hair-restoring effect.

  Proanthocyanidins are obtained by extraction and purification from plants, and can also be obtained by chemical synthesis. Plants used as raw materials for proanthocyanidins include plants such as grape genus, apple genus, barley genus, oyster genus, coco genus, cacao genus, pine genus, common bean genus, and pea genus. , Barley, oyster, palm, cacao, pine, azuki bean, peanut and the like. Proanthocyanidins are extracted from plants according to a known method, and the extraction solvent is water, alcohols such as methanol, ethanol and isopropyl alcohol, ketones such as acetone and methyl ethyl ketone, and esters such as methyl acetate and ethyl acetate. Etc. These extraction solvents may be used alone or as a mixed solvent in which a plurality of types are combined.

  As proanthocyanidins, grape seed extract proanthocyanidins, apple-derived proanthocyanidins, pine-derived proanthocyanidins, purified proanthocyanidins oligomers, procyanidins B-1, procyanidins B-2, from the viewpoint of easily exhibiting the effect as an active ingredient One kind selected from procyanidin B-3 and procyanidin C-1 is preferable. Examples of commercially available proanthocyanidins include “APC40” manufactured by Kyowa Hakko Kogyo Co., Ltd.

  The content of proanthocyanidins in the hair growth / restoration agent is 0.01 to 10% by mass, preferably 0.05 to 5% by mass, and more preferably 0.1 to 2% by mass. If this content is less than 0.01% by mass, sufficient hair-growth / hair-growth effects cannot be obtained. On the other hand, when it exceeds 10 mass%, it does not act as an active ingredient with respect to the hair-growth / hair-restoration effect. When proanthocyanidins are contained as a proanthocyanidin solution dissolved in a solvent, the content of proanthocyanidins is based on the dry mass.

  (B) Aloe extract is contained in order to exert a synergistic effect with proanthocyanidins. The aloe extract is obtained by extracting from a lily family Aloe vera, Kidachi aloe or the like as a raw material with a solvent at room temperature or under heated conditions. Examples of commercially available products of aloe extract include aloe vera liquid (manufactured by Ichimaru Falcos Co., Ltd.), aloe extract (raw materials: Kidachi Aloe, manufactured by Maruzen Pharmaceutical Co., Ltd.), and the like.

  As an extraction solvent for the aloe extract, water, alcohols such as methanol, ethanol and propanol, polyhydric alcohols such as propylene glycol and butylene glycol, ketones such as acetone and methyl ethyl ketone, esters such as methyl acetate and ethyl acetate Chain, cyclic ethers such as tetrahydrofuran and diethyl ether, halogenated hydrocarbons such as dichloromethane, chloroform and carbon tetrachloride, hydrocarbons such as hexane, cyclohexane and petroleum ether, aromatic carbonization such as benzene and toluene Examples include hydrogens, polyethers such as polyethylene glycol, and pyridines such as pyridine and methylpyridine. These extraction solvents may be used alone or as a mixed solvent in which a plurality of types are combined. Among these extraction solvents, at least selected from methanol, ethanol, hydrous methanol (methanol content 10 to 95% by mass) and hydrous ethanol (ethanol content 10 to 95% by mass) from the viewpoint of good extraction efficiency. One type is preferred.

  The content of the aloe extract in the hair growth / restoration agent is 0.0001 to 0.1% by mass, preferably 0.0005 to 0.05% by mass, more preferably 0.001 based on the dry mass of the aloe extract. -0.01 mass%. When this content is less than 0.0001 or more than 0.1% by mass, a sufficient synergistic effect is not exhibited. In particular, when the aloe extract is blended in an amount of 0.15% by mass or more, the hair-growth / hair-restoring effect is lowered as compared with the case where the aloe extract is not blended. In addition, “based on dry mass” in the content of the aloe extract is calculated from the mass after removing the solvent, that is, the dry mass of the aloe extract, when blending the aloe extract (aloe extract) containing the solvent. % By weight.

  In the hair growth / hair nourishing agent, the mass ratio of proanthocyanidins to the aloe extract [mass ratio = (dry mass of proanthocyanidins) / (dry mass of aloe extract)] is preferably 0.3 to 10,000, more preferably 1. ˜5000, more preferably 5˜1000. When this mass ratio is 0.3 to 10,000, the synergistic effect with proanthocyanidins can be exhibited more remarkably.

  (B) From the viewpoint of enhancing the synergistic effect with proanthocyanidins by using together with the aloe extract, it is preferable to further contain (C) ginseng extract. Panax ginseng extract can be obtained by extracting ginseng of the genus Arachnaceae, as a raw material, at room temperature or under warming conditions with a solvent. Examples of commercially available ginseng extract include carrot extract SK (manufactured by Koei Kogyo Co., Ltd.), Ginseng dry extract (manufactured by Indena), carrot extract (manufactured by Maruzen Pharmaceutical Co., Ltd.), and the like.

  The content of the ginseng extract in the hair growth / hair nourishing agent is 0.0001 to 0.1% by mass, preferably 0.0005 to 0.001% by mass, more preferably 0.001 based on the dry mass of the ginseng extract. It is -0.05 mass%. When this content is less than 0.0001 or more than 0.1% by mass, a sufficient synergistic effect cannot be obtained. In addition, “based on dry mass” in the content of ginseng extract is calculated from the mass after removing the solvent, that is, the dry mass of the aloe extract, when the ginseng extract containing the solvent (Ginseng extract) is blended. % By weight.

  In the hair growth and nourishing agent, the mass ratio of proanthocyanidins to the total mass of the aloe extract and ginseng extract [mass ratio = (dry mass of proanthocyanidins) / (sum of the dry mass of aloe extract and ginseng extract)] is Preferably, it is 0.2-5000, More preferably, it is 0.5-1000, More preferably, it is 2-500. When this mass ratio is 0.2 to 5000, the synergistic effect with proanthocyanidins can be more remarkably exhibited.

  In the hair growth / hair nourishing agent, the mass ratio of the ginseng extract to the aloe extract [mass ratio = (dry mass of ginseng extract) / (dry mass of aloe extract)] is preferably 0.005 to 500, more preferably. Is 0.05 to 100, more preferably 0.1 to 50. When this mass ratio is 0.005 to 500, the synergistic effect with proanthocyanidins can be exhibited more remarkably.

  The hair growth and nourishing agent includes benzalkonium chloride, salicylic acid, chlorhexidine hydrochloride, photosensitizer 201, chlorhexidine gluconate, chlorxylenol, trichlorocarbanilide, halocarbanoic acid and other fungicides, glycyrrhizic acid and Derivatives such as diphenhydramine hydrochloride, guaiazulene, carbenoxolone disodium, sicon extract, agez, l-menthol, hydrocortisone acetate, prednisolone, etc. Moisturizers such as summer grass extract and saffron extract, keratolytic agents such as salicylic acid, resorcin, and lactic acid, topical stimulants such as mint oil, estrone, estradiol, ethinyl est Follicular hormones such as diol, pyridoxine and its derivatives, anti-seborrheic agents such as sulfur, thioxolone, vanside, and lecithin, nutrients such as amino acids, cystine, cysteine, methionine, serine, vitamins, isopropyl myristate, lecithin, squalane Oils such as higher fatty acids and higher alcohols, surfactants such as polyoxyethylene sorbitan fatty acid esters, sorbitan fatty acid esters, polyoxyethylene fatty acid esters and glycerin fatty acid esters, polyhydric alcohols such as propylene glycol, glycerin and macrogol, dibutyl Effects of the present invention include antioxidants such as hydroxytoluene and isopropyl gallate, sequestering agents such as ethylenediaminetetraacetate and its salts, solvents such as water and ethanol, thickeners, dyes, and fragrances. It can be appropriately mixed within a range that does not interfere.

  Examples of the dosage form of the hair growth / restoration agent include liquid, emulsion, gel, foam, and cream. Examples of product forms of the hair growth / hair growth agent include hair tonic, hair lotion, hair cream, hair growth / hair growth shampoo, hair growth / hair growth rinse, hair gel, hair mist, hair foam, aerosol, ointment and the like.

  Now, the hair growth and hair nourishing agent is obtained by mixing the component (A), the component (B), the component (C) and other components. This hair growth and hair nourishing agent is used by applying an appropriate amount to the scalp once to several times a day. Here, when a herbal extract other than the aloe extract is blended with proanthocyanidins, it is equivalent to the hair growth / hair restoration effect of proanthocyanidins alone, or conversely, the hair growth / hair restoration effect of proanthocyanidins itself is inhibited. The hair growth and hair nourishing agent of this embodiment contains a predetermined amount of the component (A) and the component (B). That is, by including a predetermined amount of proanthocyanidins and a predetermined amount of aloe extract, an effect superior to that obtained when each of the proanthocyanidins and aloe extract is contained alone, that is, a synergistic effect is exhibited. In addition, the hair-growth / hair-growth effect in this hair-growth / hair-growth agent includes hair growth promotion, hair loss prevention, improvement of hair thickness, and increase in the amount of hair.

According to the embodiment described in detail above, the following effects are exhibited.
-The hair growth and hair nourishing agent of this embodiment contains the (A) component and the (B) component. While content of (A) component is 0.01-10 mass%, content of (B) component is 0.0001-0.1 mass% on the basis of dry mass. Therefore, the component (A) and the component (B) can act synergistically to exert a synergistic effect on hair growth / hair growth. As a result, the hair growth / hair restoration effect can be enhanced.

  -It is preferable that the hair growth and hair nourishing agent of this embodiment contains 0.0001-0.1 mass% of (C) component on the basis of dry mass. By using a predetermined amount of the component (C) together with the component (B), a synergistic effect on hair growth / hair growth can be further exhibited. As a result, it is possible to further enhance the hair growth and nourishing effect.

In addition, the said embodiment can also be changed and comprised as follows.
-The component (C) may be omitted.
-As herbal extracts other than the (B) component and the (C) component, for example, a essence extract, a garlic extract, etc. may be blended within a range that does not inhibit the action of the (B) component and the (C) component.

  -When blending the (A) component, the (B) component, and the (C) component, they may be blended in a liquid form dissolved in a solvent, or in a dry state after removing the solvent, that is, in a solid form such as a powder. Also good.

Next, the technical idea that can be grasped from the above embodiment will be described below.
(B) the proanthocyanidins, the general formula (1) Ru compounds der flavan-7-ol derivative is polymerized represented by education wool baldness remedy.

(B) the mass ratio of the proanthocyanidin for aloe extract [mass ratio = (dry weight of proanthocyanidins) / (dry weight of aloe extract)] is 0.3 to 10000 der Ru education wool baldness remedy.

(C) the mass ratio of said Panax extract on aloe extract [mass ratio = (dry weight of the ginseng extract) / (dry weight of aloe extract) is 0.05 to 500 der Ru education wool hair tonic .

Next, the embodiment will be described more specifically with reference examples, prescription examples and comparative examples.
( Reference Example 1 and Formulation Example 2, Comparative Examples 1-7)
Each component shown in Table 1 was mixed to prepare a hair growth and nourishing agent. The unit of the numerical value indicating the blending amount of each component in Table 1 is mass%, and the mass% of proanthocyanidins, aloe extract and ginseng extract is a value based on dry mass.

The hair growth and hair nourishing agents in each case were confirmed by the human epithelial keratinocyte growth test described below for hair growth and hair growth effects.
<Human epithelial keratinocyte proliferation test>
1) Human epithelial keratinocytes (HEK, manufactured by Toyobo Co., Ltd.) were cultured in a 60 mm dish (Type I collagen coated) in Growth medium.

2) Trypsinization was performed at the time of confluence, and then a cell suspension was prepared in Growth medium, and cells were seeded in a 96-well microplate (Type I collagen coated) at a cell concentration of 4 × 10 ³ / well. .

3) Cultured for 1 day in Growth medium.
4) The hair growth / hair nourishing agent of each example was dissolved in a basic medium so as to be 0.01% by mass, and then sterilized by filtration with a 0.22 μm filter.

  5) After removing the human epithelial keratinocyte culture medium cultured in a 96-well microplate and washing with phosphate buffer [PBS (−)], add 100 μl each of the hair-growth and nourishing agent-containing medium obtained in 4) above. did.

6) The cells were cultured for 2 days at 37 ° C. and 5% CO ₂ .
7) Add 10 μl of Growth medium containing 5 mg / ml MMT [3- (4,5-dimethylthiazoyl-yl) -2,5-diphenyltetrazolium bromide] reagent to the hair growth / hair nourishing agent-containing medium, The cells were cultured at 37 ° C. and 5% CO ₂ for 4 days.

8) Formazan extract (0.04N isopropanol hydrochloric acid diluted 300 times with isopropanol) was added in 100 ml portions and shaken.
9) The absorbance at 570 nm was measured using a microplate reader. On the other hand, an experiment in which no hair growth / hair growth agent was added as a control was performed in the same manner, and the degree of cell growth in the hair growth / hair growth agent of each example was calculated with the cell growth degree obtained from the absorbance at this time being 100.

表１の結果から明らかなように、参考例１及び処方例２では細胞増殖度の値が１５０以上となり、育毛・養毛効果が十分に発揮されることがわかる。特に、処方例２では（Ｂ）成分に加えて（Ｃ）成分が含有されているため、細胞増殖度の値が１９０以上となり、育毛・養毛効果が顕著に発揮されている。

As is clear from the results in Table 1, it can be seen that in Reference Example 1 and Formulation Example 2, the value of the degree of cell proliferation is 150 or more, and the hair growth / hair restoration effect is sufficiently exhibited. In particular, in Formulation Example 2, since the component (C) is contained in addition to the component (B), the value of the degree of cell proliferation is 190 or more, and the hair growth / hair restoration effect is remarkably exhibited.

Here, the value of the cell proliferation degree in Reference Example 1 is 26 or more higher than the value of the cell proliferation degree in Comparative Examples 1 and 2 each containing proanthocyanidins and an aloe extract alone. From these results, it can be seen that a synergistic effect on hair growth and hair restoration can be obtained by using the component (A) and the component (B) in combination.

  In Comparative Example 3, since the aloe extract is not contained, the value of the cell proliferation degree is equivalent to that of Comparative Example 1 containing proanthocyanidins alone. In Comparative Examples 4 to 7, the concentration of proanthocyanidins or the concentration of aloe extract is out of the predetermined concentration range. Therefore, the value of the degree of cell proliferation in Comparative Examples 4 to 7 is equal to or lower than that of Comparative Example 1 in which proanthocyanidins are added alone.

Claims (1)

(A) Proanthocyanidins and (B) Aloe extract and (C) Panax ginseng extract as active ingredients, (A) Proanthocyanidins are contained in an amount of 0.05 to 2% by mass, and (B) Aloe The extract contains 0.0001 to 0.1% by mass based on the dry mass, and the (C) ginseng extract contains 0.0001 to 0.1% by mass based on the dry mass.・ Hair nourishing agent .