JP4290841B2 - Melanocyte dendrite elongation inhibitor and cosmetics containing the same - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a melanocyte dendrite elongation inhibitor and an external preparation for cosmetics and the like, and more specifically, a melanocyte and macrophage suitable for suppressing the interaction between melanocytes and macrophages with a melanocyte dendrite elongation inhibitor. The present invention relates to an external preparation for skin for dealing with skin phenomena.
[0002]
[Prior art]
It is already known that melanocytes are the leading role of life phenomena related to pigments in animals, but melanin, which is such a pigment, is produced in melanocytes and how it moves to epidermal cells. It is not yet known in detail, but it is only known that macrophages are often involved in the movement of such melanin granules. It has been pointed out that the involvement of such macrophages is caused by producing melanocyte dendrite elongation factor (DEF). No attempt has been made to suppress the growth of melanocytes and to suppress the movement of melanin granules to prevent the skin from becoming darkened by suppressing the extension of dendrites. Furthermore, it is not known at all that the essence of peach, which is a plant of the Rosaceae family, has such a melanocyte elongation-inhibiting action.
[0003]
On the other hand, resolving pigment abnormalities caused by abnormalities in melanin granules produced by melanocytes is a long-standing problem for humankind to embody beautiful white skin. Various mechanisms have been obtained, but none has focused on the suppression of melanocyte dendrite elongation. It is not known at all that it is useful for the prevention and improvement of pigmented dysfunctions associated with. In addition, melanin production inhibitors such as ascorbic acid, which are well known in the art, are not yet effective against pigment abnormalities associated with inflammation and pigment abnormalities such as buckwheat, and a means for preventing or improving such pigment abnormalities. Development was desired.
[0004]
In addition, it is already known that the essence of rosaceae peach has antibacterial and anti-inflammatory effects, but this has the effect of suppressing the dendritic extension of melanocytes. It is not known at all, and therefore, a topical skin preparation such as cosmetics containing this suppresses dendrite elongation of melanocytes, and therefore, abnormal pigmentation, particularly, abnormal pigmentation caused by inflammation involving light. It is not known at all to be useful in the prevention and improvement of the disease.
[0005]
[Problems to be solved by the invention]
The present invention has been made under such circumstances, and it is an object of the present invention to provide an effective preventive or ameliorating means for pigment abnormalities such as pigment abnormalities accompanied by inflammation and buckwheat.
[0006]
[Means for solving problems]
In view of such a situation, the present inventors have sought for effective preventive or remedy measures for pigment abnormalities such as inflammation and pigment abnormalities such as buckwheat. By finding an excellent essence of melanocyte dendrite elongation-inhibiting action and adding a substance having such action to an external preparation for skin, such an external preparation for skin causes abnormalities in pigmentation such as inflammation and pigmentation such as buckwheat. They found it useful for prevention and improvement, and completed the invention. That is, the present invention relates to the following technique.
(1) A melanocyte dendrite elongation inhibitor comprising an extract of a petal of a Rosaceae peach petal with a polar solvent .
(2) The melanocyte elongation inhibitor according to (1 ), wherein dendrite elongation inhibition is caused by an inhibitory action of a macrophage-derived dendrite elongation promoting factor.
( 3 ) A skin external preparation for dealing with skin phenomena involving melanocytes and macrophages, comprising the melanocyte elongation inhibitor according to any one of (1) to (2).
( 4 ) A skin external preparation for dealing with skin phenomena involving melanocytes and macrophages according to ( 3 ), which is a cosmetic.
( 5 ) The skin phenomenon involving melanocytes and macrophages is skin darkening or buckwheat accompanied by light-induced inflammation. ( 3 ) or ( 4 ) For dealing with skin phenomena involving melanocytes and macrophages. Topical skin preparation.
( 6 ) A cosmetic for whitening, characterized by containing an extract of petals of rosaceae peaches and / or a solvent-removed product of the extract .
Below, the present invention will be described in detail focusing on embodiments.
[0007]
DETAILED DESCRIPTION OF THE INVENTION
(1) Melanocyte Dendrite Elongation Inhibitor of the Present Invention The melanocyte dendrite elongation inhibitor of the present invention comprises the essence of a rose family peach. Here, the essence refers to a plant body of the plant itself, a processed product obtained by drying, chopping, pulverizing the plant body, an extract obtained by extracting the plant body or the processed product with a solvent, and a solvent of the extract. It means a general term for the removed solvent-removed product, the extract or the purified fraction obtained by purifying the solvent-removed product by column chromatography or liquid-liquid extraction. Among these, as the dendrite elongation inhibitor of the melanocyte of the present invention, a solvent extract or a solvent removed product thereof can be preferably exemplified, and a polar solvent can be particularly preferably exemplified as such a solvent. Examples of such polar solvents include alcohols such as water, ethanol, methanol, 1,3-butanediol, and propylene glycol, esters such as ethyl acetate and methyl formate, ketones such as acetone and methyl ethyl ketone, chloroform, and the like. Preferred examples include one or more selected from halogenated hydrocarbons such as methylene chloride, nitriles such as acetonitrile, and ethers such as diethyl ether and tetrahydrofuran. Of these, water and / or alcohols are particularly preferable. In order to prepare such an extract, a 1 to 10-fold amount of a solvent is added to a plant body or a processed product, and it is immersed for several days at room temperature or for several hours at a temperature near the boiling point. Thereafter, the insoluble matter is removed by filtration or the like, and the solvent can be removed by vacuum concentration or lyophilization as necessary. As a part of the plant body used for such solvent extraction, there is no particular limitation because an effective substance is distributed throughout the plant body, but a part other than the xylem can be preferably exemplified, and among them the petal part, Many such components are contained, which is particularly preferable. The essence of rosaceae peach, which is the melanocyte dendrite elongation inhibitor of the present invention thus obtained, is excellent in the action of inhibiting melanocytes from stretching dendrite, so that melanin granules are transferred from melanocytes to the skin tissue. It has the action of preventing or improving pigment abnormalities such as blackening and buckwheat accompanied by inflammation, which occurs at the time of light irradiation, which suppresses movement and has such a mechanism of movement of melanin granules. Such an action is considered to be based on the mechanism by which the dendritic elongation factor of melanocytes released by macrophages inhibits melanocytes from acting. Of course, since pigment abnormalities take such a route in the production of melanin granules, the dendritic elongation inhibitor of melanocytes of the present invention also suppresses pigment abnormalities other than blackening and buckwheat accompanied by inflammation caused by light irradiation. Since such pigment abnormalities can be prevented or ameliorated by other means, the effect of the present invention is characterized by preventing or improving pigment abnormalities such as blackening or buckwheat accompanied by inflammation caused by the light irradiation. It can be said that it works.
[0008]
(2) Skin external preparation for dealing with skin phenomenon involving melanocytes and macrophages of the present invention The dendritic elongation inhibitor of melanocytes of the present invention inhibits melanocyte dendritic elongation factor released from macrophages from acting on melanocytes. Therefore, it is possible to suppress the life phenomenon in which melanocytes and macrophages work in a coordinated manner. By including such a dendritic elongation inhibitor of melanocytes in a topical skin preparation, melanocytes and macrophages are involved. Can cope with skin phenomenon. That is, the external preparation for skin of the present invention is for dealing with skin phenomena involving melanocytes and macrophages, and is characterized by containing the dendrite elongation inhibitor of melanocytes of the present invention. Here, the topical skin preparation as used in the present invention is a general term for compositions applied externally to the skin, and can be preferably exemplified by a skin external medicine containing a patch and a cosmetic containing a cleansing agent. Then, it is especially preferable that it is cosmetics. This is because the melanocyte dendrite elongation inhibitor of the present invention has high safety and mild action. As the skin phenomenon involving melanocytes and macrophages, particularly preferably, the above-mentioned pigmentation abnormality such as blackening or buckwheat accompanied by inflammation caused by light irradiation is mentioned as the most important problem, but other inflammatory reactions are also included. . In the skin external preparation for dealing with skin phenomena involving melanocytes and macrophages of the present invention, the preferred content of the dendritic elongation inhibitor for melanocytes is 0.001% by weight to 10% by weight with respect to the total amount of the external preparation for skin. More preferably, it is 0.01% by weight to 5% by weight. This may outgrowth inhibition effect of too small dendrites not exhibited, because the effect is too large, it may undermine the freedom of the other ingredients become plateaued.
[0009]
The skin external preparation for dealing with skin phenomena involving melanocytes and macrophages according to the present invention can contain any components that are usually used in cosmetics and external skin medicines in addition to the above essential components. Such optional components include, for example, hydrocarbons such as squalane, petrolatum, microcrystalline wax, esters such as jojoba oil, carnauba wax, octyldodecyl oleate, triglycerides such as olive oil, beef tallow, coconut oil, stearic acid, etc. , Fatty acids such as oleic acid, lithinoleic acid, higher alcohols such as oleyl alcohol, stearyl alcohol, octyldodecanol, anionic surfactants such as sulfosuccinic acid ester and sodium polyoxyethylene alkylsulfate, and amphoteric surfactants such as alkylbetaine salts Agents, cationic surfactants such as dialkylammonium salts, sorbitan fatty acid esters, fatty acid monoglycerides, polyoxyethylene adducts thereof, polyoxyethylene alkyl ethers, polyoxyethylenes Nonionic surfactants such as fatty acid esters, polyhydric alcohols such as polyethylene glycol, glycerin, 1,3-butanediol, thickening / gelling agents, antioxidants, ultraviolet absorbers, colorants, preservatives , Powder and the like can be contained. Of course, the inclusion of hydroquinones such as ascorbic acids and arbutin, which are conventional melanin production inhibitors, may also exhibit a synergistic effect and is advantageous.
[0010]
【Example】
Hereinafter, the present invention will be described in more detail with reference to examples, but it is needless to say that the present invention is not limited to these examples.
[0011]
<Example 1>
Add 5 liters of water to 500 g of rose petal petals, heat at 90 ° C. with stirring for 2 hours, cool to room temperature, filter to remove insoluble matter, and remove the dendrite elongation inhibitor 1 for melanocytes of the present invention. Obtained.
[0012]
<Example 2> Using the dendrite elongation inhibitor 1 for melanocytes of Example 1, the dendrite elongation inhibitory action was examined. That is, according to a conventional method, macrophages were collected from the mouse abdominal cavity and diluted with a minimal medium containing 10% FBS-added eagle to prepare a macrophage solution at a concentration of 2 × 10 ⁶ cells / ml. 90 μl of this was dispensed into a 35 mm petri dish and irradiated with ultraviolet rays at 0.05 mW / cm ² for 20 minutes. These dendrites elongation inhibitor for melanocytes were cultured overnight at added 37 ° C. dissolved in a solvent. Also, cut the tail of the other mouse, finely cut the tail epidermis into a petri dish, treat with 0.5% trypsin at 37 ° C overnight, separate it into epidermis and dermis using tweezers, and collect only the epidermis. The solution was treated with 0.5% trypsin at 37 ° C. for 20 minutes, and only melanocytes were collected as a filtrate by filtration. The filtrate containing this melanocyte was cultured at 37 ° C. for 48 hours in a medium in which 10% FBS, 10 ⁻⁴ M IBMX and 10 ng / ml TPA were added to Eagle's minimal medium. This was suspended in the same medium, dispensed at 1000 cells / well into 96-wells, and cultured overnight at 37 ° C. The melanocyte medium was discarded, washed 3 times with PBS, and replaced with 35 μl of minimal medium with 10% FBS-added eagle. To this was added 35 μl each of the culture supernatant of macrophages containing the specimen, cultured at 37 ° C. overnight, photographed under an optical microscope, and the length of dendrites was measured from this photograph. The results are shown in FIG. From this, it can be seen that the dendrite elongation inhibitor of melanocytes of the present invention is excellent in dendrite elongation-inhibiting action.
(Sample)
1) Dimethyl sulfoxide solution of melanocyte dendrite elongation inhibitor 1 (0.0005%)
2) Dimethyl sulfoxide (positive control)
3) Do not add macrophage supernatant (negative control)
[0013]
<Example 3>
Toxicity of the melanocyte dendrite elongation inhibitor 1 to melanocytes was screened by counting the number of cells, but it was equivalent to the vehicle, dimethyl sulfoxide, and no toxicity to melanocytes was observed.
[0014]
<Example 4>
Toxicity of the melanocyte dendrite elongation inhibitor 1 to macrophages was screened by MTT assay, but it was equivalent to the vehicle, dimethyl sulfoxide, and no toxicity to macrophages was observed.
[0015]
<Example 5>
A lotion was prepared according to the formulation shown below. That is, the prescription ingredients were heated and dissolved at 80 ° C., stirred and cooled to obtain a lotion. Using this lotion, the effect of improving blackening accompanied by inflammation caused by ultraviolet irradiation was investigated. That is, four sites of 2 cm × 4 cm were provided on the inner side of the upper arm of the person, and ultraviolet irradiation of 1.5 times the minimum amount of erythema was applied to these sites for 2 days to cause blackening accompanied by inflammation. ΔL values of these parts with respect to the standard white plate were measured, and the following lotion and the dendrite elongation inhibitor 1 in the following lotion were replaced with water (control product), and the dendrite elongation inhibitor 1 Was replaced with sodium ascorbate (Comparative product 1) and dendrite elongation inhibitor 1 was replaced with glutathione (Comparative product 2). Measured, and from these, (ΔL value after treatment) − (ΔL value before treatment) was calculated. The control product was 0.5, the lotion of the present invention was 1.3, and the comparative product 1 was 0.7. The comparative product 2 was 0.6, and it was found that the skin lotion which is an external preparation for skin of the present invention has an excellent improvement effect on blackening accompanied by inflammation caused by ultraviolet irradiation.
Melanocyte dendrite elongation inhibitor 1 0.1 parts by weight glycerin 5 parts by weight sulfated trehalose sodium 0.1 part by weight heparin analog 0.1 part by weight ethanol 5 parts by weight 1,3-butanediol 5 parts by weight methylparaben 0.1 Parts by weight water 84.6 parts by weight
<Example 6 > An emulsion was prepared according to the formulation shown below. That is, the prescription ingredients A, B, and C are heated and dissolved at 80 ° C., and after gradually adding and emulsifying B, the mixture is gradually added and neutralized, and the emulsified particles are prepared with a homogenizer, stirred and cooled, An emulsion was obtained. This thing was excellent in the suppression effect of buckwheat.
I)
Squalane 10 parts by weight cetanol 1.5 parts by weight isostearic acid triglyceride 5 parts by weight stearic acid 1 part by weight sorbitan sesquistearate 1.5 parts by weight polyoxyethylene behenyl ether 1 part by weight butylparaben 0.1 part by weight
B )
Melanocyte dendrite elongation inhibitor 1 0.5 part by weight sulfated trehalose sodium 0.1 part by weight heparin analog 0.1 part by weight 1,3-butanediol 5 part by weight methylparaben 0.1 part by weight carboxyvinyl polymer 0.3 Parts by weight water 40 parts by weight c)
Potassium hydroxide 0.2 parts by weight Water 33.6 parts by weight
【The invention's effect】
ADVANTAGE OF THE INVENTION According to this invention, the effective prevention or improvement means can be provided with respect to pigment abnormalities accompanying inflammation, pigment abnormalities, such as buckwheat.
[Brief description of the drawings]
FIG. 1 is a diagram showing the results of Example 2. FIG.

Claims (5)

Melanocyte dendrite elongation inhibitor consisting of an extract of polar petals from the rose petal. 2. The melanocyte elongation inhibitor according to claim 1, wherein dendrite elongation inhibition is caused by an inhibitory action of a macrophage-derived dendrite elongation promoting factor. A skin external preparation for dealing with skin phenomena involving melanocytes and macrophages, comprising the melanocyte elongation inhibitor according to any one of claims 1 to 2 ,
A skin external preparation, wherein the skin phenomenon is a skin blackening phenomenon or buckwheat accompanied by light-induced inflammation. The skin external preparation for skin phenomena involving melanocytes and macrophages according to claim 3, which is a cosmetic. A cosmetic for whitening comprising an extract of petals of a rose family peach and / or a solvent-removed product of the extract.

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