JP4236538B2 - バイオセンサ - Google Patents
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- JP4236538B2 JP4236538B2 JP2003291150A JP2003291150A JP4236538B2 JP 4236538 B2 JP4236538 B2 JP 4236538B2 JP 2003291150 A JP2003291150 A JP 2003291150A JP 2003291150 A JP2003291150 A JP 2003291150A JP 4236538 B2 JP4236538 B2 JP 4236538B2
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Description
本発明は、液体試料が導入されるキャビティと前記キャビティ内より外部に通じる空気孔とを具備し、導入された前記液体試料と試薬の反応により液体試料中の成分を分析可能なバイオセンサについてである。すなわち、全体の主要構成は図1に示したものと同等の構成であるが、少なくともキャビティの外表面における空気孔の周辺部が化学的な処理を施すことで撥水性を有することに特徴がある。そして空気孔の周辺部の表面自由エネルギーが43mN/m以下であることに特徴がある。
プラスチックフィルムの材質としては、ポリエステル、ポリオレフィン、ポリアミド、ポリエステルアミド、ポリエーテル、ポリイミド、ポリアミドイミド、ポリスチレン、ポリカーボネート、ポリ−ρ−フェニレンスルフィド、ポリエーテルエステル、ポリ塩化ビニル、ポリ(メタ)アクリル酸エステルが挙げられる。また、これらの共重合体やブレンド物やさらに架橋したを用いることもできる。 さらに、上記プラスチックフィルムの中でも、ポリエステル、例えば、ポリエチレンテレフタレート、ポリエチレン2,6−ナフタレート、ポリエチレンα,β−ビス(2−クロルフェノキシ)エタン4,4’−ジカルボキシレート、ポリブチレンテレフタレートなどが好ましく、これらの中で機械的特性、作業性などの品質、経済性などを総合的に勘案すると、ポリエチレンテレフタレートが特に好ましく用いられる。
(1)表面自由エネルギー
表面自由エネルギーおよびその各成分(分散力、極性力、水素結合力)が既知の4種の液体(本発明ではPanzerによる方法IV(日本接着協会誌vol.15、No.3、p96に記載の水、エチレングリコール、ホルムアミド、ヨウ化メチレンの数値を用いた)を用い、20℃、50%RHの条件下で接触角計CA−D型(協和界面科学(株)製)にて各液体での接触角を測定した。この値を拡張Fowkes式とYoungの式より導入される下記式を用いて各成分を計算した。
(γSd・γLd)1/2+(γSp・γLp)1/2+(γSh・γLh)1/2=γL(1+cosθ)/2
ここでγLd、γLp、γLh、γLは測定液の分散力、極性力、水素結合力の各成分および各成分のトータルの表面自由エネルギーを示し、γSd、γSp、γSh は測定面上の分散力、極性力、水素結合力の各成分を示す。また、θは測定面上での測定液の接触角を表す。1つの測定面に対し5個測定を行いその平均値をθとした。既知の値およびθを上記の式に代入し、連立方程式により測定面の3成分を求める。なお計算は数値計算ソフトである「Mathematica」の「FindMinimum」のコマンドを用いた。
塗布液を塗布した基材100cm2の重量を測定し(A)、次に塗布前の基材100cm2の重量を測定し(B)、(A−B)×100で付着量(g/m2)を算出した。
次に、各部材の作製方法について以下に説明する。
(1)スペーサ
東レ(株)製ポリエチレンテレフタレートフィルム”ルミラー”(タイプ100E20)を基材とした。
東レ(株)製ポリエチレンテレフタレートフィルム“ルミラー”(タイプ100T60)面に付加反応型シリコーン(東レダウコーニングシリコーン製LTC350G)を0.1g/m2塗布したものを基材とし、パンチングプレスにてφ0.5mmの空気孔を設けた。(空気孔の周辺部の表面自由エネルギーは12.0mN/mであった)
東レ(株)製ポリエチレンテレフタレートフィルム“ルミラー”(タイプ100T60)にポリエステルフィルム製造工程中にアクリル系樹脂を0.1g/m2塗布した(インラインコート法)ものを基材とし、パンチングプレスにてφ0.5mmの空気孔を設けた。(空気孔の周辺部の表面自由エネルギーは41.0mN/mであった)
東レ(株)製ポリエチレンテレフタレートフィルム“ルミラー”(タイプ100T60)を基材とし、パンチングプレスにてφ0.5mmの空気孔を設けた。(空気孔の周辺部の表面自由エネルギーは46.9mN/mであった)
東レ(株)製ポリエチレンテレフタレートフィルム“ルミラー(タイプ250H10)”5上に、スクリーン印刷法により作用極1及び対極2からなる電極を設け、その上に酵素(グルコースオキシダーゼ)、電子伝達体(フェリシアン化カリウム)および親水性高分子(カルボキシメチルセルロース)などを含む試薬層10を形成し、その上から、前記各部材の作製方法(1)の手順で作製した切り欠け部を有するスペーサ7と、前記各部材の作製方法(2)または(3)または(4)の手順で作製したカバーAまたはカバーBまたはカバーCとを接着することで、血液が導かれるキャビティを備えた血糖センサを作製した。空気孔9はφ0.5mmとし、各々のカバーの裏面(キャビティと接する面)には界面活性剤をコーティングした。
2 対極
3 作用極用リード
4 対極用リード
5 絶縁基板
6 カバー
7 スペーサ
8 吸引口
9 空気孔
10 試薬層
11 キャビティ
Claims (4)
- 液体試料が導入されるキャビティと、該キャビティ内より外部に通じる空気孔とを具備し、導入された前記液体試料と試薬との反応により液体試料中の成分を分析可能なバイオセンサにおいて、少なくとも前記キャビティの外表面における前記空気孔の周辺部が化学的な処理を施すことで撥水性を有していることを特徴とするバイオセンサ。
- 基板とカバーによりキャビティが構成され、該キャビティは、液体試料の入口とその入口より毛細管現象にて液体試料の導入を促進する為の空気孔とを備え、導入された前記液体試料と試薬との反応により液体試料中の成分を分析可能なバイオセンサにおいて、前記キャビティを構成する基板またはカバーの外表面のうち、少なくとも前記空気孔の周辺部が化学的な処理を施すことで撥水性を有していることを特徴とするバイオセンサ。
- 前記化学的な処理が撥水性の高い物質を塗布することであることを特徴とする請求項1または2に記載のバイオセンサ。
- 前記撥水性の高い物質が、シリコーンオイル、シリコーン系、ハイドロカーボン系、フルオロカーボン系、ワックス系、ポリエチレンイミンーオクタデシルイソシアネート系やポリ(メタ)アクリル酸エステル系、ポリスチレン系、ポリエチレン系、ポリプロピレン系樹脂であることを特徴とする請求項3記載のバイオセンサ。
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