JP4184414B1 - Production method of barley syrup - Google Patents

Abstract

The present invention provides a method for producing barley syrup using barley as a raw material, in which the viscosity of the obtained syrup is sufficiently low, and a food and medium containing barley syrup obtained by the production method.
A method for producing barley syrup comprising a decomposition step of decomposing barley or a pulverized product thereof at 20 to 65 ° C. in the presence of α-amylase. In the decomposition step, an enzyme such as β-glucanase, pullulanase, or protease may coexist.
[Selection figure] None

Description

  The present invention relates to a method for producing barley syrup.

Conventionally, syrup (waterpox) mainly made of cereals such as barley has been used as an additive such as mirin or a raw material for sparkling sake. As a method for producing such a syrup, for example, a method including a liquefaction step, a saccharification step, and a protein degradation step described in Patent Document 1, a method including a liquefaction step and a saccharification step described in Patent Document 2, and the like are known. .
JP 2006-262839 A JP 2005-323556 A

  However, the conventional syrup manufacturing methods including the methods described in Patent Documents 1 and 2 have a problem that the syrup has a high viscosity during manufacturing, and handling of the resulting syrup becomes difficult.

  Therefore, the present invention has been made in view of the above circumstances, the viscosity of the resulting syrup is sufficiently low, a method for producing barley syrup using barley as a raw material, and a food containing barley syrup obtained by the production method and An object is to provide a medium.

This invention provides the manufacturing method of barley syrup provided with the decomposition | disassembly process which decomposes | disassembles barley or its ground material at 20-65 degreeC in alpha-amylase presence, and obtains a saccharified liquid .

  According to this production method, the temperature in the decomposition step is set to a range of 20 to 65 ° C., and the presence of α-amylase suppresses the increase in the molecular weight of β-glucan, and the viscosity of the resulting syrup is sufficiently low. can do.

  Furthermore, in the conventional method for producing barley syrup, β-amylase contained in the raw barley as a raw material is deactivated in the liquefaction step, and therefore β-amylase must be newly added after the liquefaction step. On the other hand, in the production method of the present invention, the temperature is in the range of 20 to 65 ° C., so that the activity of β-amylase contained in barley is sufficiently maintained. Therefore, sugar can be produced without newly adding an enzyme such as β-amylase, and barley syrup can be produced with a smaller number of steps than in the past. Therefore, the manufacturing method of the present invention can reduce the manufacturing cost and improve the production efficiency.

  In the decomposition step, pullulanase is preferably allowed to coexist in order to hydrolyze the α-1,6-glucoside bond (branched portion) in starch and to generate sugar more efficiently. In the production method of the present invention, since the temperature is in the range of 20 to 65 ° C., the degradation with α-amylase and the degradation with pullulanase can be performed simultaneously. Therefore, according to the manufacturing method of the present invention, the manufacturing cost can be reduced and the production efficiency can be improved.

  Moreover, in the said decomposition | disassembly process, in order to decompose | disassemble the protein derived from the barley of a raw material, and to produce | generate an amino acid, it is preferable to make protease coexist. In the production method of the present invention, since the temperature is in the range of 20 to 65 ° C., the degradation with α-amylase and the degradation with protease can be simultaneously performed, and the production cost can be reduced.

  Moreover, in the said decomposition | disassembly process, in order to decompose | disassemble the beta-glucan contained in barley or its ground material, and to make the viscosity of the obtained syrup further lower, it is preferable to make beta-glucanase coexist. In the production method of the present invention, since the temperature is in the range of 20 to 65 ° C., the degradation with α-amylase and the degradation with β-glucanase can be performed simultaneously. Therefore, according to the manufacturing method of the present invention, the manufacturing cost can be reduced and the production efficiency can be improved.

  This invention provides the foodstuff containing the barley syrup obtained by the above-mentioned manufacturing method. Since the barley syrup obtained by the above-mentioned production method has a low viscosity, it can be suitably used for a wide variety of foods. Examples of the “food” include solid ingredients such as bread, yogurt, cheese, confectionery, snacks, seasonings such as miso, vinegar, miso, soy sauce, butter, and soft drinks, sake, beer, and sparkling sake. And beverages such as shochu.

  Moreover, this invention provides the culture medium containing the barley syrup obtained by the above-mentioned manufacturing method. By using the barley syrup obtained by the above-described production method as a fermentation medium, a new fermented food utilizing the functional substance contained in the barley can be produced.

  ADVANTAGE OF THE INVENTION According to this invention, the viscosity of the syrup obtained can provide the food and culture medium containing the barley syrup obtained by the manufacturing method of the barley syrup which uses barley as a raw material, and the said manufacturing method. Furthermore, according to the manufacturing method of the present invention, the manufacturing cost can be reduced and the production efficiency can be improved.

  Hereinafter, preferred embodiments of the present invention will be described.

  In a preferred embodiment of the production method according to the present invention, first, barley or a pulverized product thereof is decomposed in water in the presence of α-amylase to obtain a saccharified solution (decomposition step). In this decomposition step, carbohydrates such as starch in the raw barley are decomposed into low molecular sugars.

  As barley, all kinds of barley such as Nijo, Rojo, bare, and leather can be used. Moreover, as a barley used as a raw material, all tissues and fractions derived from barley seeds such as whole grains, wheat grains, and straw can be used.

  In the present invention, syrup is produced from barley as a raw material, but is not necessarily limited thereto. For example, cereals such as wheat, oats, oats, rye, and rice are used alone or in combination with barley. Can also be used.

(Α-amylase)
As the α-amylase, conventionally known ones can be used. For example, commercially available amylase AD “Amano” 1 (manufactured by Amano Enzyme), Christase T10S (manufactured by Yamato Kasei Co., Ltd.), Christase YC15S (Yamato) Kasei Co., Ltd.) can be used. The amount of α-amylase added can be appropriately adjusted in accordance with the activity of α-amylase, for example, 0.01 to 1 part by mass with respect to 100 parts by mass in total of barley or a pulverized product thereof. can do. As the α-amylase, an enzyme in which β-glucanase activity is mixed can also be used.

(Enzyme)
Furthermore, in the decomposition step, β-glucanase is preferably allowed to coexist in order to decompose the raw barley-derived β-glucan and further reduce the viscosity. A conventionally known β-glucanase can be used. When β-glucanase is allowed to coexist, the amount added is preferably 0.01 to 1 part by mass with respect to 100 parts by mass in total of barley or a pulverized product thereof.

  Furthermore, in the decomposition step, pullulanase may coexist in order to hydrolyze the α-1,6-glucoside bond (branched portion) in the starch derived from barley as a raw material and to generate sugar more efficiently. preferable. A conventionally known pullulanase can be used. When pullulanase is allowed to coexist, the amount added is preferably 0.01 to 1 part by mass with respect to 100 parts by mass in total of barley or a pulverized product thereof. In addition, as a pullulanase, the enzyme in which (beta) -glucanase activity is mixed can also be used.

  Furthermore, in the decomposition step, it is preferable to coexist with a protease in order to decompose the raw barley-derived protein and produce amino acids. As the protease, conventionally known ones can be used. When the protease is allowed to coexist, the amount added is preferably 0.01 to 1 part by mass with respect to 100 parts by mass in total of barley or a pulverized product thereof. The barley syrup obtained by the production method of the present invention contains many amino acids such as GABA and glutamic acid, and can be suitably used for various functional foods, fermentation media and the like. In addition, as a protease, an enzyme in which β-glucanase activity is mixed can also be used.

  When enzymes such as β-glucanase, pullulanase and protease coexist, the order of adding these enzymes is not particularly limited, and may be added simultaneously with α-amylase, or may be added before or after α-amylase is added. . Further, at least two of α-amylase, β-glucanase, pullulanase and protease may be mixed and the mixture may be added.

(Disassembly process)
In the decomposition step, the temperature at which the barley or the pulverized product thereof is decomposed is 20 to 65 ° C, preferably 45 to 65 ° C, and more preferably 50 to 60 ° C. When this temperature is less than 20 ° C., the decomposition of carbohydrates such as starch in the raw barley is insufficient. Moreover, when this temperature is 20 degreeC or more, carbohydrates, such as a starch in the raw barley, are decomposed | disassembled. Furthermore, when this temperature is 65 ° C. or lower, the viscosity of the resulting syrup is lowered.

  Moreover, the reaction time in a decomposition | disassembly process can be suitably adjusted according to the activity of α-amylase, a reaction scale, etc., for example, can be 30 minutes-24 hours.

  The density | concentration of the barley used for a decomposition | disassembly process is 0.5-80 mass% with respect to water, and it is preferable in it being 2-60 mass%, and it is more preferable in it being 2.5-40 mass%.

  The decomposition step may be a batch type or a continuous type. In the case of a batch type, it may be appropriately stirred during the decomposition step. In the case of a continuous type, raw barley and water are mixed in advance, and decomposed while being heated at a predetermined temperature and residence time while being fed by a pump to obtain a saccharified solution.

(Post-decomposition process)
Next, the insoluble part is removed from the saccharified solution obtained in the above-described decomposition step by centrifugation or filter press. Furthermore, the target soluble barley syrup can be obtained by filtering the remaining soluble part with diatomaceous earth, activated carbon or the like as an auxiliary agent, and further purifying by performing fine filtration.

(Pretreatment process)
In addition, the above-mentioned manufacturing method may be provided with the pre-processing process which pre-processes barley or its ground material at 20-40 degreeC before a decomposition | disassembly process.

  In the pretreatment step, barley or a pulverized product thereof is reacted in water at 20 to 40 ° C. for 30 minutes to 24 hours, for example. Thereby, endogenous enzymes in barley can be activated, and the content of amino acids such as GABA and glutamic acid, proteins, peptides and the like in the obtained barley syrup can be improved.

(Barley syrup)
The barley syrup obtained by the above production method contains amino acids such as GABA and glutamic acid. In addition, the kind and content of amino acids contained in barley syrup can be changed by appropriately adjusting the kind of raw barley and the kind of enzyme used in the decomposition step.

  Moreover, the obtained barley syrup can be appropriately processed to a state suitable for the application. Examples of such treatment include concentration treatment and sterilization / powdering treatment. In particular, when barley syrup having a high viscosity obtained by a conventional production method is used, sterilization / powdering treatment is difficult. According to the barley syrup obtained by the production method of the present invention, such sterilization / powdering treatment can be easily performed.

(Use of barley syrup)
Barley syrup obtained by the above-mentioned production method is, for example, solid ingredients such as bread, yogurt, cheese, confectionery, snacks, seasonings such as miso, vinegar, miso, soy sauce, butter, and soft drinks, sake, beer It can be suitably used for foods such as beverages such as happoshu and shochu.

  Moreover, the new barley syrup obtained by the above-mentioned manufacturing method can manufacture the new fermented food which utilized the functional substance of barley by using it as a fermentation culture medium.

  EXAMPLES Hereinafter, although an Example demonstrates this invention further in detail, this invention is not limited to this.

[Preparation of crushed barley]
CDC Fibar (produced in Canada in 2006) was crushed with a cyclone mill in the form of whole grains and used as a raw material for barley syrup. In addition, 50 g of this barley pulverized product was placed on a 75 μm mesh, 150 μm mesh, 300 μm mesh, 600 μm mesh, 1000 μm mesh, and 2000 μm mesh sieve and placed on a sieve for 5 minutes. Diameter measurement was performed. The obtained results are shown in FIG. Further, when the crude protein ratio of the pulverized barley was measured by the Kjeldahl method, the value was 18.5% in terms of anhydrous.

[Enzyme preparation]
As α-amylase, Christase YC15S (trade name, manufactured by Daiwa Kasei Co., Ltd.), as protease, protease S “Amano” G (trade name, manufactured by Amano Enzyme Co., Ltd.) as β-amylase, Tokyo Chemical Industry Co., Ltd. The pullulanase manufactured by Amano Enzyme Co., Ltd. was prepared using β-amylase manufactured by the company as the pullulanase. In the following examples, 25 mg of these enzymes were dissolved in 1000 μl of H ₂ O, and 40 μl of the solution was used.

(Example 1)
Put _H 2 O40ml to 50ml Falcon tube, and incubated (preheating) of _{H 2} O to 50 ° C.. To this, 1.0 g of the barley pulverized product described above was added, α-amylase was added to 0.1% (w / w%) of barley, and the mixture was shaken for 4 hours with a shaker in an air incubator maintained at 50 ° C. Decomposed (decomposition process). Thereafter, the barley syrup of Example 1 was obtained by quenching with an ice bath, centrifuging at 800 rpm for 15 minutes, and filtering the supernatant with a filter paper (manufactured by ADVANTEC).

(Example 2)
A barley syrup of Example 2 was obtained in the same manner as in Example 1 except that protease was added in addition to the α-amylase described above.

(Example 3)
Barley syrup of Example 3 was obtained in the same manner as Example 1 except that the incubation temperature and the internal temperature of the air incubator were changed from 50 ° C to 60 ° C.

Example 4
Barley syrup of Example 4 in the same manner as in Example 1 except that the incubation temperature and the internal temperature of the air incubator were changed from 50 ° C. to 60 ° C. and that protease was added in addition to the α-amylase described above. Got.

[Evaluation 1 of barley syrup]
The barley syrup obtained in Examples 1 to 4 was measured for viscosity, filtration rate, β-glucan concentration, SN (Soluable Nitrogen), amino acid concentration, and Brix.

(Measurement of viscosity)
For barley syrup obtained in Examples 1 to 4, using an Ubbelohde viscometer, the viscosity was measured at 20.00 ° C. undiluted samples diluted neat sample or H _{2 O.} The results are shown in Table 1.

(Evaluation of filtration rate)
The time required for the filtration in Examples 1 to 4 described above was measured. Table 1 shows the results of evaluation as “A” when the filtration time is within 15 minutes, “B” when 15 to 30 minutes, and “C” when it exceeds 30 minutes.

(Measurement of β-glucan concentration)
The barley syrups obtained in Examples 1 to 4 were each diluted 7.5 times with H ₂ O, and then filtered with a 0.45 μm filter. The concentration of β-glucan was measured. The results are shown in Table 1.
Two high-pressure pumps:
Shodex (Showa Denko) DS-4 (H ₂ O 1.0 ml / min.)
HITACHI L-6000 Pump (reaction solution 2.0 ml / min.)
Auto sampler:
No. 1: System Instruments Co., Ltd. Autosampler Model AS-09
No. 2: System Instruments Co., Ltd. Autosampler Model 33
Fluorescence detector: Spectral fluorescence detector RF-10AXL for Shimadzu high performance liquid chromatograph (excitation wavelength 360 nm, fluorescence wavelength 420 nm)
Column thermostat: Shodex (Showa Denko KK) OVEN AO-30C
Deaeration device: ERC Co., Ltd. ERC-3215
Data processor: System Instruments Co., Ltd. Chromatocoder21
Mixing coil: round Teflon tube with an inner diameter of 0.5 mm and an empty volume of 0.5 ml to a diameter of 7 cm. Gel filtration column:
Shodex SUGAR BT-603
Column size: 6φ × 50mm
Column end connection screw: male screw type, no. 10-32UNF
Column material: SUS 316
Filler: Polyhydroxymethacrylate Exclusion limit molecular weight: 1 × 10 ⁵ (Pullane)

(Measurement of SN)
About the filtrate of the barley syrup obtained in Examples 1-4, SN was measured by the Kjeldahl method. The results are shown in Table 1.

(Measurement of Brix)
For the barley syrup filtrates obtained in Examples 1-4, Brix was measured with ATAGO RX-5000. The results are shown in Table 1.

(Measurement of amino acid concentration)
The barley syrup obtained in Examples 1 to 4 was filtered with Ultracel YM-10 Regenerated Cellulose 10,000 MWCO (manufactured by MILLIPORE), and the filtrate was diluted with H ₂ O and AccQ · FLUOR REAGENT KIT (manufactured by Waters) ) And derivatized by the AccQ · Tag method, and the concentration of amino acids was measured. The obtained results are shown in Table 1. In the table, “total a.a.” represents all free amino acids of protein constituent amino acids excluding undetectable tryptophan, “GABA” represents γ-aminobutyric acid, and “Glu” represents glutamic acid. . Moreover, the following apparatus was used for the measurement.
Apparatus: 2695 separation module, column heater, 2475 multi-λ fluorescence detector, Empower personal mobile phase A: 166 mM sodium acetate, 5.6 mM triethylamine (pH 5.7)
Mobile phase B: 166 mM sodium acetate, 5.6 mM triethylamine (pH 6.8)
Mobile phase C: Acetonitrile mobile phase D: H ₂ O
Column: AccQ-Tag Amino Acid Analysis Column (3.9 × 150 mm) + Sentry Nova C18
Column temperature: 39 ° C
Injection volume: 39 μL
Detection: Ex 250 nm Em 395 nm GAIN 10

(Example 5)
Put _H 2 O40ml to 50ml Falcon tube, and incubated (preheating) of _{H 2} O to 60 ° C.. To this, 1.0 g of the barley pulverized product described above was added, α-amylase was added to 0.1% (w / w%) of barley, and the mixture was shaken for 24 hours with a shaker in an air incubator maintained at 60 ° C. Decomposed (decomposition process). Thereafter, the barley syrup of Example 5 was obtained by quenching with an ice bath, centrifuging at 800 rpm for 15 minutes, and filtering the supernatant with a filter paper (manufactured by ADVANTEC).

(Example 6)
A barley syrup of Example 6 was obtained in the same manner as Example 5 except that β-amylase was added in addition to the α-amylase described above.

(Example 7)
A barley syrup of Example 7 was obtained in the same manner as in Example 5 except that pullulanase was added in addition to the α-amylase described above.

(Example 8)
Barley syrup of Example 8 was obtained in the same manner as Example 5 except that β-amylase and pullulanase were added in addition to the above α-amylase.

Example 9
Barley syrup of Example 9 was obtained in the same manner as Example 5 except that β-amylase, pullulanase and protease were added in addition to the above α-amylase.

[Evaluation 2 of barley syrup]
About the barley syrup obtained in Examples 5 to 9, the β-glucan concentration, amino acid concentration, Brix, and sugar concentration were measured. Note that the β-glucan concentration, amino acid concentration, and Brix were measured by the same method as in the evaluation 1 of the barley syrup described above. The results are shown in Table 2.

(Measurement of sugar concentration)
The filtrate of barley syrup obtained in Examples 5 to 9 was heat-treated at 100 ° C. for 10 minutes and then rapidly cooled in an ice bath. This was centrifuged at 15000 rpm and 5 ° C. for 15 minutes, and the supernatant was diluted with 0.1% benzoic acid and subjected to measurement of the sugar concentration. The results are shown in Table 2. The following apparatus was used for measuring the sugar concentration.
Device: DIONEX DX-300
Mobile phase A: 0.1M sodium hydroxide
Mobile phase B: 0.1M sodium hydroxide, 1M sodium acetate
Column: CarboPac PA1
Injection volume: 15 μl

(Example 10)
Put _H 2 O400ml in 500ml tubes and incubated (preheating) of _{H 2} O to 60 ° C.. 50 g of the above-mentioned barley pulverized product was added thereto, α-amylase, β-amylase and pullulanase were added to each barley 0.1% (w / w%) and the internal temperature was kept at 60 ° C. The shaker in the air incubator For 24 hours to decompose (decomposition process). Thereafter, the barley syrup of Example 10 was obtained by quenching with an ice bath, centrifuging at 800 rpm for 15 minutes, and filtering the supernatant with a filter paper (manufactured by ADVANTEC).

(Comparative Example 1)
Put _H 2 O400ml in 500ml tubes and incubated (preheating) of _{H 2} O to 55 ° C.. To this, 50 g of the barley pulverized product described above was added, α-amylase was added to 0.05% (w / w%) of barley, and the mixture was shaken for 1 hour with a shaker in an air incubator maintained at 55 ° C. Thereafter, the temperature was raised to 90 ° C. over 1 hour, and 0.05% (w / w%) of α-amylase was added to the barley and reacted for 1 hour to obtain a liquefied liquid. Next, the obtained liquefied liquid was cooled to 60 ° C., β-amylase was added at 0.1% (w / w%) of barley, and reacted at 60 ° C. for 24 hours to obtain a saccharified liquid. Thereafter, protease was added to barley 0.1% (w / w%) and reacted at 60 ° C. for 24 hours. The barley syrup of Comparative Example 1 was obtained by filtering the reaction solution through filter paper.

[Evaluation 3 of barley syrup]
About the barley syrup obtained in Example 10 and Comparative Example 1, the viscosity, the concentration of β-glucan, SN and Brix were measured in the same manner as in the case of the barley syrup evaluation 1 described above. The obtained results are shown in Table 3.

It is a bar graph which shows the result of having performed the particle size measurement of the pulverized product of barley.

Claims (6)

A method for producing barley syrup, comprising a decomposition step of decomposing barley or a pulverized product thereof at 20 to 65 ° C in the presence of α-amylase to obtain a saccharified solution .   The method for producing barley syrup according to claim 1, wherein pullulanase is allowed to coexist in the decomposition step.   The method for producing barley syrup according to claim 1 or 2, wherein a protease is allowed to coexist in the decomposition step.   The method for producing barley syrup according to any one of claims 1 to 3, wherein β-glucanase is allowed to coexist in the decomposition step.   The foodstuff containing the barley syrup obtained by the manufacturing method of Claims 1-4.   The culture medium containing the barley syrup obtained by the manufacturing method of Claims 1-4.

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