JP3840491B2 - Sample liquid measuring device - Google Patents

Description

  The present invention relates to a sample liquid measuring device for measuring a toxic substance present in a sample.

Today, there are many substances (broadly toxic substances) that are harmful to living organisms in the environment that surrounds us. For example, in addition to trihalomethane in tap water and dioxins in the atmosphere, pollution of raw water and groundwater by so-called toxic substances such as cyan and cadmium is also popular in newspapers. In order to detect such poisons in real time, an “ecosensor” using a lipid bilayer membrane as shown in FIG. 9 has been proposed (see, for example, Patent Document 1). The eco-sensor has a plurality of water tanks 117 and includes micropores 104 in which a lipid bimolecular film capable of acting on poisons is formed on a part of the substrate thin film 103 that is a tank partition wall. By taking the potential difference of the liquid with the reference electrode 133, it is possible to sense the poisonous substance. In addition, since the lipid bilayer membrane deteriorates over time and the sensitivity becomes weak, it is necessary to recreate the membrane immediately before measurement in the actual sensor. In order to realize this, the lipid liquid stored in the storage tank 131 is discharged into the micropores 104 by the discharge unit 130 using an ink jet mechanism, and a lipid bilayer membrane is automatically formed.
JP 2001-91494 A (paragraph number “0008”, FIG. 3)

  However, the lipid that has not been used for forming the lipid bilayer membrane is attached to the vicinity of the micropores 104 or the water tank 117, and there has been a problem of inhibiting the formation of the lipid bilayer membrane. As shown in FIG. 10, among the lipid liquid 105 discharged from the lipid discharge nozzle 102, the lipid liquid that was not used for film formation in the micropores 104 adheres to the substrate thin film 103 as dirt 106 due to the lipid liquid. It was left.

  In view of the above problems, an object of the present invention is to provide a sample liquid measuring device capable of ensuring the stability and reproducibility of a lipid bilayer by removing unnecessary lipid liquid or lipid. .

  In order to achieve the above object, the first feature of the present invention is that the sample liquid storage section, the reference liquid storage section, and the sample liquid storage section and the reference liquid storage section are positioned between the sample liquid and the reference liquid. It has a substrate thin film with micropores in which a lipid bilayer film is in contact with each other and a mechanism for forming a lipid bilayer film by discharging a lipid solution, and measuring the potential difference between the sample solution and the reference solution. And a sample liquid measuring device for detecting a lysate in the sample liquid, the sample liquid measuring device including an unnecessary lipid liquid adhering to the micropores or a mechanism for ejecting a cleaning liquid to the lipid. To do. Here, the “mechanism for discharging a lipid solution to form a lipid bilayer” refers to, for example, a lipid discharge nozzle that discharges a lipid solution by a jet water flow or the like. The “mechanism for ejecting the cleaning liquid onto the unnecessary lipid liquid or lipid attached to the micropores” refers to, for example, a cleaning nozzle that ejects the cleaning liquid by a jet water flow or the like.

  According to the sample liquid measuring apparatus according to the first feature of the present invention, unnecessary lipid liquid or lipid attached to the micropores can be removed, and stability and reproducibility of the lipid bilayer membrane can be ensured. .

  In the sample liquid measuring apparatus according to the first feature, the temperature of the cleaning liquid when reaching the unnecessary lipid liquid or lipid may be equal to or higher than the dissolution temperature of the lipid. According to this sample liquid measuring device, the effect of washing is further increased by dissolving the lipid.

  Furthermore, in the sample liquid measuring device according to the first feature, when the cleaning liquid is ejected to the unnecessary lipid liquid or lipid, the water level of the sample liquid and the reference liquid may be lower than the micropores. According to this sample liquid measuring device, the water flow from the mechanism that ejects the cleaning liquid to the unnecessary lipid liquid or lipid attached to the micropores is not lost to the liquid in the aquarium, and kinetic energy is not lost to the liquid while keeping the momentum. Cleaning can be performed.

  The second feature of the present invention is that the sample liquid storage section, the reference liquid storage section, and the lipid bimolecule located between the sample liquid storage section and the reference liquid storage section and in contact with both the sample liquid and the reference liquid A substrate thin film having micropores formed with a membrane and a mechanism for discharging a lipid solution to form a lipid bilayer, and measuring the potential difference between the sample solution and the reference solution to dissolve the solution in the sample solution It is a sample liquid measuring device for detecting the above, and a sample liquid measuring device provided with a mechanism provided on a substrate thin film that heats the micropores to a melting temperature of the lipid or higher. Here, the “mechanism provided on the substrate thin film that heats the micropores above the melting temperature of the lipid” refers to, for example, a thin film heater attached to the substrate thin film.

  According to the sample measuring apparatus according to the second feature, it is possible to remove unnecessary lipid liquid or lipid adhering to the periphery of the micropores, and to ensure the stability and reproducibility of the lipid bilayer membrane.

  The third feature of the present invention is that the sample liquid storage section, the reference liquid storage section, and the lipid bimolecule located between the sample liquid storage section and the reference liquid storage section and in contact with both the sample liquid and the reference liquid A substrate thin film having micropores formed with a membrane and a mechanism for discharging a lipid solution to form a lipid bilayer, and measuring the potential difference between the sample solution and the reference solution to dissolve the solution in the sample solution It is a sample liquid measuring device for detecting a sample liquid, and a sample liquid measuring device including a mechanism provided on a substrate thin film that vibrates a micropore. Here, the “mechanism provided on the substrate thin film that vibrates the micropores” refers to, for example, a thin film vibrator attached to the substrate thin film.

  According to the sample measuring apparatus according to the third feature, it is possible to remove unnecessary lipid liquid or lipid adhering to the periphery of the micropore, and to ensure the stability and reproducibility of the lipid bilayer membrane.

  The fourth feature of the present invention is that the sample liquid container, the reference liquid container, and the lipid bimolecule that is located between the sample liquid container and the reference liquid container and in which both the sample liquid and the reference liquid are in contact with each other A substrate thin film having micropores formed with a membrane and a mechanism for discharging a lipid solution to form a lipid bilayer, and measuring the potential difference between the sample solution and the reference solution to dissolve the solution in the sample solution The gist of the present invention is a sample solution measuring device for detecting a sample solution, comprising a mechanism for heating the sample solution and the reference solution to a temperature higher than the dissolution temperature of the lipid. Here, the “mechanism for heating the sample solution and the reference solution to a temperature equal to or higher than the dissolution temperature of the lipid” refers to, for example, a heater provided at the sample solution inlet or a heater provided at the sample solution storage unit.

  According to the sample measuring apparatus according to the fourth feature, unnecessary lipid liquid or lipid existing in the water tank including the sample liquid storage part and the reference liquid storage part can be removed, and the stability and reproduction of the lipid bilayer membrane. Sex can be secured.

  The fifth feature of the present invention is that the sample liquid container, the reference liquid container, and the lipid bimolecule that is located between the sample liquid container and the reference liquid container and in which both the sample liquid and the reference liquid are in contact with each other A substrate thin film having micropores formed with a membrane and a mechanism for discharging a lipid solution to form a lipid bilayer, and measuring the potential difference between the sample solution and the reference solution to dissolve the solution in the sample solution The gist of the present invention is a sample liquid measuring device for detecting a sample liquid, comprising a mechanism for stirring the sample liquid or the reference liquid.

Here, the “mechanism for stirring the sample liquid or the reference liquid” refers to, for example, a stirrer disposed in the sample liquid storage part or the reference liquid storage part.

  According to the sample measuring apparatus according to the fifth feature, unnecessary lipid solution or lipid existing in the water tank including the sample solution storage unit and the reference solution storage unit can be removed, and the stability and reproduction of the lipid bilayer membrane can be achieved. Sex can be secured.

  According to the present invention, an object is to provide a sample liquid measuring device capable of ensuring the stability and reproducibility of a lipid bilayer by removing unnecessary lipid liquid or lipid.

  Next, first to fourth embodiments of the present invention will be described with reference to the drawings. In the following description of the drawings, the same or similar parts are denoted by the same or similar reference numerals. However, it should be noted that the drawings are schematic.

(First embodiment)
In the first embodiment, a sample measurement device that removes excess lipid solution or lipid attached around the micropores of the substrate thin film will be described. In the first embodiment, a cleaning liquid is discharged into the micropores to remove unnecessary lipids.

  As shown in FIG. 1, the sample liquid measuring device according to the first embodiment is positioned between the sample liquid storage unit 21, the reference liquid storage unit 22, and the sample liquid storage unit 21 and the reference liquid storage unit 22. And a substrate thin film 3 having micropores 4 formed with a lipid bimolecular film in contact with both the sample liquid and the reference liquid, a lipid ejection nozzle 2 and a cleaning nozzle 1.

  An electrode 6 is provided inside the sample solution storage unit 21, and this electrode 6 is inserted into the sample solution. Similarly, an electrode 7 is installed inside the reference liquid storage unit 22, and this electrode is inserted into the reference liquid. With these electrodes 6 and 7, the potential difference between the sample solution and the reference solution separated by the lipid bilayer membrane (that is, the membrane potential) can be measured by a measuring device 8 such as an electrometer. It has become. Data obtained by the measuring device 8 can be recorded by the recorder 9.

  The sample liquid storage unit 21 is configured so that sample liquid can be injected and discharged. The sample liquid is injected and discharged through, for example, at least one flow port provided in the wall surface of the sample liquid storage unit 21. After injecting the sample liquid from the flow port into the sample liquid storage unit 21 and measuring the sample liquid, it is possible to discharge the measured sample liquid from this flow port or a different flow port. In the sample liquid measuring apparatus according to the first embodiment, by providing a plurality of flow ports and providing a sample liquid injection port and a discharge port, injection and discharge of the sample liquid into and from the sample liquid storage unit 21 are performed simultaneously. It is preferable to carry out continuously. As shown in FIG. 1, if a sample liquid inlet 15 is provided in the lower part of the sample liquid container 21 and a sample liquid outlet 16 is provided in the upper part, a liquid flow of the sample liquid is generated around the lipid bilayer. Thus, the sample liquid comes into continuous contact with the lipid bilayer membrane. In this way, it is possible to continuously detect the lysate in the sample liquid by continuously contacting the lipid bilayer membrane with the sample liquid as a liquid flow and measuring the potential difference between the sample liquid and the reference liquid. become.

  On the other hand, the reference liquid storage unit 22 can also be configured so that the reference liquid can be injected and discharged. Preferably, as shown in FIG. 1, if a reference liquid inlet 25 is provided in the lower part of the reference liquid container 22 and a reference liquid outlet 26 is provided in the upper part, a liquid flow in the reference liquid container 22 is generated. Can do.

The substrate thin film 3 is located between the sample liquid storage part 21 and the reference liquid storage part 22, for example, Teflon (registered trademark, poly-tetra-fluoro-ethylene (—CF ₂ —CF ₂ —) having a thickness of about 25 μm. n). The substrate thin film 3 has one or more micropores 4, and a lipid bimolecular film is formed in the micropores 4.

  The lipid ejection nozzle 2 is an inkjet device, and ejects a lipid liquid to form a lipid bilayer film in the micropores 4. At this time, the substrate thin film 3 on which the lipid bimolecular film is formed becomes a target of lipid droplets by the ink jet mechanism and is therefore contaminated with lipid. It has been confirmed by experiments that the lipid bimolecular film becomes difficult to form when the substrate thin film 3 is soiled with lipid. Therefore, it is necessary to clean the substrate thin film 3 just before forming the lipid bilayer.

  The cleaning nozzle 1 shown in FIGS. 1 and 2 is configured to wash away dirt due to a lipid liquid adhering to the substrate thin film 3 by a jet water flow using the cleaning liquid. It is desirable to wash the lipid solution at regular intervals or immediately before measuring the sample solution. Moreover, the washing | cleaning liquid to be used can use arbitrary things, as long as it can wash away a lipid bilayer. Suitable cleaning liquids include water, warm water and the like, and the solvent of the sample liquid and the sample liquid or the reference liquid can be used as the cleaning liquid in some cases.

  Moreover, generally the lipid used with an ecosensor melt | dissolves at 20-30 degreeC. For this reason, if the temperature of the jet water flow when reaching the substrate thin film 3 is equal to or higher than the melting temperature, it is clear that the cleaning effect is further increased. In order to make the cleaning liquid have a lipid dissolution temperature, for example, the cleaning liquid nozzle 1 can be provided with a heating device such as a heating wire.

  In addition, it is clear that the jet water flow that flows at the time of washing increases the washing effect of attached lipids as the momentum increases. In FIG. 3, the level of the sample liquid in the water tank is lowered during cleaning. By washing the micropores 4 in the air in this way, the jet water flow from the washing nozzle 1 can wash the lipid while maintaining its momentum without losing kinetic energy to the sample liquid in the water tank. it can.

  It is preferable that the cleaning nozzle 1 is installed in the rising liquid flow in the sample liquid storage unit 21 or the reference liquid storage unit 22 so that the cleaning of dirt by lipids is performed in the rising liquid flow. Usually, the lipid bilayer membrane formation solution has a specific gravity lower than that of the sample solution. However, in this case, the lipid bilayer membrane washed away, excess membrane formation solution generated during film formation or dirty washing solution is contained. This is because it is promoted to float to the liquid surface of the part, and it is easy to discharge them outside the system.

  The rising liquid flow can be generated by any suitable method. For example, as shown in FIG. 1, the sample liquid inlet 15 is provided at the lower part of the sample liquid container 21 and the sample liquid outlet 16 is provided at the upper part to raise the sample liquid around the micropores 4 with dirt. Can be done. In addition, a flow path can be provided inside the sample liquid storage portion 21 with a plate or the like to generate a liquid flow that rises around the contaminated micropores 4. Thereby, the cleanliness of the substrate thin film 3 is ensured.

  In FIG. 1, excess lipid bilayer membrane formation liquid and dirty cleaning liquid floating on the surface of the sample liquid are discharged from the sample liquid discharge port 16. The discharged liquid can be collected by a separating means such as a filter 14 as necessary.

  It should be noted that the lipid bilayer membrane 18 according to the first embodiment has some changes in the lipid bilayer membrane caused by the action of dissolved substances in the sample solution (for example, membrane potential, electric capacity, ion permeability, luminescence, heat generation). , Changes in endotherm, etc.) to detect the presence and concentration of dissolved substances in the sample liquid. Examples of such lipid bilayer membranes include lipid bilayer membranes substantially composed only of lipids and lipid bilayer membranes in which molecules such as various proteins and sugars are attached or blended. By appropriately selecting the type and amount of lipid, protein, and sugar, and the method for producing the lipid bilayer, various sensors can be manufactured according to the specific purpose of the measurement purpose or sample solution. Examples of the lipid bilayer membrane suitable for the sample measuring apparatus according to the first embodiment include those obtained by attaching or blending a protein or sugar such as an antibody to a pseudolipid such as monoolein or triolein or a phospholipid. .

  According to the sample liquid measuring apparatus according to the first embodiment, unnecessary lipid liquid or lipid attached to the micropores 4 can be removed using the cleaning nozzle 1. As a result, the stability and reproducibility of the lipid bilayer membrane can be ensured, and maintenance-free and real-time wide-range poison detection and quantification is possible.

(Second Embodiment)
In the second embodiment, as in the first embodiment, a sample measurement device that removes excess lipid solution attached around the micropores of the substrate thin film will be described. In the second embodiment, unnecessary lipids are removed by heating or vibrating the substrate thin film.

  The substrate thin film 3 of the sample liquid measuring apparatus according to the second embodiment includes a thin film heater 11 around the microhole 4 as shown in FIG. The thin film heater 11 is made of metal or ceramics. In addition, since the substrate thin film 3, the lipid ejection nozzle 2 and the like are the same as those in the first embodiment, description thereof is omitted here. The thin film heater 11 is energized by the power source 12 to heat the substrate thin film 3 and dissolve the dirt 10 due to the lipid liquid adhering to the substrate thin film 3. Thereby, unnecessary lipids around the micropores 4 can be removed. As described in the first embodiment, excess lipid bilayer membrane formation liquid and dirty cleaning liquid floating on the surface of the sample liquid are discharged from the sample liquid discharge port. The discharged liquid can be collected by a separating means such as a filter as necessary.

  The sample liquid measuring apparatus according to the second embodiment shown in FIG. 4 includes the cleaning nozzle 1 described in the first embodiment in addition to the thin film heater 11, and uses both the dissolution temperature and the jet water flow. Unnecessary lipid solution or lipid may be removed.

  Further, the substrate thin film 3 of the sample liquid measuring apparatus according to the second embodiment may include a thin film vibrating body 13 around the microhole 4 as shown in FIG. The thin-film vibrating body 13 may be formed by attaching a vibrating body such as a piezoelectric element to the substrate thin film 3 or forming a microhole in the thin vibrating body itself. In addition, ultrasonic waves may be used. The thin film vibrating body 13 can release the dirt 10 due to the lipid liquid adhering to the substrate thin film 3 by vibrating the substrate thin film 3.

  The sample liquid measuring apparatus according to the second embodiment shown in FIG. 5 includes the cleaning nozzle 1 described in the first embodiment in addition to the thin-film vibrating body 13, and uses both vibration and jet water flow. Unnecessary lipid solution or lipid may be removed.

  Furthermore, it is needless to say that the substrate thin film 3 of the sample liquid measuring device according to the second embodiment may include both the substrate thin film 3 thin film heater 11 and the thin film vibrating body 13.

  According to the sample liquid measuring apparatus according to the second embodiment, unnecessary lipid liquid or lipid adhering to the periphery of the micropore 4 can be removed using the thin film heater 11 or the thin film vibrating body 13. As a result, the stability and reproducibility of the lipid bilayer membrane can be ensured, and maintenance-free and real-time wide-range poison detection and quantification is possible.

(Third embodiment)
In the third embodiment, a sample measurement device that removes excess lipid liquid adhering not only to the vicinity of the micropores of the substrate thin film but also to the entire water tank will be described. In the third embodiment, unnecessary lipid is removed by heating the sample solution or the reference solution to a temperature higher than the dissolution temperature of the lipid.

  As shown in FIG. 6, the sample liquid measuring apparatus according to the third embodiment includes a heater 19 a in the sample liquid injection port 15, heats the sample liquid introduced during cleaning with the heater 19 a, and introduces it into the water tank 17. To do. The reference liquid inlet 25 is provided with a heater 19b, and the reference liquid introduced at the time of cleaning is heated by the heater 19b and introduced into the water tank 17.

In addition, since the lipid ejection nozzle 2, the substrate thin film 3, the sample liquid discharge port 16, the sample liquid storage part 21, the reference liquid storage part 22 and the like are the same as those in the first embodiment, description thereof is omitted here. The heater 19a heats the sample solution above the lipid dissolution temperature, and the heater 19b heats the reference solution above the lipid dissolution temperature. As described above, the lipid dissolves when the temperature rises above a certain temperature, so that the dirt of the lipid liquid existing in the water tank 17 is dissolved, and unnecessary lipid can be removed. As described in the first embodiment, the excess lipid bilayer membrane formation liquid and the dirty cleaning liquid floating on the surface of the sample liquid are discharged from the sample liquid discharge port 16. The discharged liquid can be collected by a separating means such as a filter as necessary. Similarly, the discharged liquid discharged from the reference liquid discharge port 26 can also be collected by a separating means such as a filter. In FIG. 6, the heaters 19a and 19b are provided in both the sample liquid inlet 15 and the reference liquid inlet 25. However, only one of the heaters may be provided.

  Further, as shown in FIG. 7, the heater 19 may be arranged in the sample liquid and heat the sample liquid and the reference liquid in the water tank 17.

  The sample liquid measuring device according to the third embodiment includes the cleaning nozzle 1 described in the first embodiment in addition to the heaters 19, 19a, 19b, and uses both the dissolution temperature and the jet water flow. Unnecessary lipid solution or lipid may be removed. Furthermore, the substrate thin film 3 of the sample liquid measuring apparatus according to the third embodiment includes the thin film heater 11 or the thin film vibrating body 13 described in the second embodiment, and the whole water tank and the substrate thin film 3 are heated or vibrated. May be used.

  According to the sample solution measuring apparatus according to the third embodiment, unnecessary lipid solution or lipid existing in the water tank 17 is dissolved and removed by heating the sample solution and the reference solution with the heaters 19, 19a, 19b. can do. As a result, the stability and reproducibility of the lipid bilayer membrane can be ensured, and maintenance-free and real-time wide-range poison detection and quantification is possible.

(Fourth embodiment)
In the fourth embodiment, as in the third embodiment, a sample measurement device that removes excess lipid liquid adhering not only to the vicinity of the micropores of the substrate thin film but also to the entire water tank will be described. In the fourth embodiment, unnecessary lipids are removed by stirring the sample solution or the like with a stirrer.

  As shown in FIG. 8, the sample liquid measurement device according to the fourth embodiment includes a stirrer 20 inside a sample liquid storage unit 21. In addition, since the substrate thin film 3 and the like are the same as those in the first embodiment, the description thereof is omitted here. The stirrer 20 stirs the sample liquid in the water tank 17 to cause convection to release unnecessary lipid liquid or lipid. At this time, not only the lipid on the substrate thin film 3 but also the effect of washing out the whole lipid in the water tank 17 can be expected. As described in the first embodiment, excess lipid bilayer membrane formation liquid and dirty cleaning liquid floating on the surface of the sample liquid are discharged from the sample liquid discharge port. The discharged liquid can be collected by a separating means such as a filter as necessary. In FIG. 8, the stirrer is arranged inside the sample liquid storage unit 21, but it goes without saying that the stirrer may be arranged inside the reference liquid storage unit 22.

  The sample liquid measurement device according to the fourth embodiment includes the cleaning nozzle 1 described in the first embodiment in addition to the stirrer 20, and uses both convection and jet water flow to remove unnecessary lipid liquid or Lipid removal may be performed. Further, the substrate thin film 3 of the sample solution measuring apparatus according to the fourth embodiment includes the thin film heater 11 or the thin film vibrating body 13 described in the second embodiment, and not only the convection but also the melting temperature or vibration. May be used. Moreover, the sample liquid measuring device according to the fourth embodiment includes the heaters 19, 19a, 19b described in the third embodiment, and may use not only convection but also heating of the entire water tank 17. .

  According to the sample solution measuring apparatus according to the fourth embodiment, unnecessary lipid solution or lipid existing in the water tank 17 can be removed by stirring the sample solution or the reference solution with the stirrer 20. As a result, the stability and reproducibility of the lipid bilayer membrane can be ensured, and maintenance-free and real-time wide-range poison detection and quantification is possible.

(Other embodiments)
Although the present invention has been described according to the above-described embodiments, it should not be understood that the descriptions and drawings constituting a part of this disclosure limit the present invention. From this disclosure, various alternative embodiments, examples and operational techniques will be apparent to those skilled in the art.

  For example, in the first to fourth embodiments, the cleaning nozzle 1, the lipid ejection nozzle 2, the heater 19, the stirrer 20, and the like have been described with reference to the drawings disposed in the sample liquid storage unit 21. Of course, these may be arranged in the reference liquid storage unit 22.

  In addition, the sample measurement devices according to the first to fourth embodiments may include a combination of the elements described in the respective embodiments. For example, the cleaning nozzle 1, the thin film vibrator 13, and the heaters 19, 19a, 19b may be selected and provided. Each element described in the first to fourth embodiments can be appropriately selected according to the type of sample liquid and the actual situation of measurement.

  As described above, the present invention naturally includes various embodiments not described herein. Therefore, the technical scope of the present invention is defined only by the invention specifying matters according to the scope of claims reasonable from the above description.

It is a schematic diagram of the sample liquid measuring apparatus which concerns on 1st Embodiment. It is a schematic diagram of the nozzle for washing | cleaning of the sample liquid measuring device which concerns on 1st Embodiment. It is a figure at the time of lowering the water level of the sample liquid measuring device concerning a 1st embodiment. It is an enlarged view of the substrate thin film of the sample liquid measuring device concerning a 2nd embodiment (the 1). It is an enlarged view of the substrate thin film of the sample liquid measuring device concerning a 2nd embodiment (the 2). It is a schematic diagram of the sample liquid measuring apparatus which concerns on 3rd Embodiment (the 1). It is a schematic diagram of the sample liquid measuring apparatus which concerns on 3rd Embodiment (the 2). It is a schematic diagram of the sample liquid measuring device which concerns on 4th Embodiment. It is a schematic diagram of the conventional sample liquid measuring apparatus. FIG. 9 is an enlarged view around the micropore in FIG. 8.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Cleaning nozzle 2 Lipid discharge nozzle 3 Substrate thin film 4 Micropore 5 Lipid liquid 6, 7 Electrode 8 Measuring device 9 Recorder 10 Dirt by lipid liquid 11 Thin film heater 12 Power supply 13 Thin film vibrator 14 Filter 15 Sample liquid injection port 16 Sample liquid outlet 17 Water tank 18 Lipid bilayer membrane 19, 19a, 19b Heater 20 Stirrer 21 Sample liquid container 22 Reference liquid container 25 Reference liquid inlet 26 Reference liquid outlet 103 Substrate thin film 104 Micropore 105 Lipid liquid 106 Lipid Dirt due to liquid 117 Water tank 121 Sample liquid storage part 122 Reference liquid storage part 130 Discharge part 131 Storage tank 132 Controller 133 Electrode

Claims (4)

A micropore in which a sample liquid storage part, a reference liquid storage part, and a lipid bilayer membrane located between the sample liquid storage part and the reference liquid storage part and in which both the sample liquid and the reference liquid are in contact are formed And a mechanism for ejecting a lipid solution to form the lipid bilayer, and measuring a potential difference between the sample solution and the reference solution to detect a lysate in the sample solution A sample liquid measuring device for
Sample liquid measuring device, characterized in that it comprises a machine structure provided on the substrate film for heating the microporous above the melting temperature of the lipid. Sample liquid measurement apparatus according to claim 1, further comprising a mechanism provided in the substrate on a thin film for vibrating the front Symbol micropores. Before SL sample liquid and the sample liquid measuring apparatus according to the reference liquid to claim 1 or claim 2, further comprising a mechanism for heating above the melting temperature of the lipid. Before SL sample liquid or sample liquid measurement apparatus according to any one of claims 1 to 3, further comprising a mechanism for stirring the reference solution.

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