JP3806819B2 - Cosmetic liquid and its manufacturing method - Google Patents

Description

  The present invention relates to a cosmetic liquid that effectively contains hyaluronic acid, heat-denatured collagen, arginine, and ascorbic acid, and a method for producing the same.

  In addition to fibronectin extracted from the living body, the cosmetic composition disclosed in Patent Document 1 further contains an alkali-treated collagen type III or a mixture of an alkali-treated collagen type I and type III Is.

  The cosmetic disclosed in Patent Document 2 contains sesame oil and one or more compounds selected from hyaluronic acid or a salt thereof, collagen and elastin.

  Patent Document 3 discloses a cosmetic containing eicosapentaenoic acid and / or docosahexaenoic acid or an oil containing the same, and one or more compounds selected from hyaluronic acid or a salt thereof, collagen, and elastin. .

  Patent Document 4 states that cosmetics contain natural melanin that is readily soluble in alkali and one or more components selected from a biological moisturizing component, a plant extract, a tissue extract component, an antioxidant, and a whitening agent. Disclose. Further, it is disclosed that the biological moisturizing component is composed of one or more selected from hyaluronic acid and a salt thereof, collagen and elastin.

Patent Document 5 discloses a skin cell active substance that activates skin cells by internal use and contains at least three components of type I collagen peptide, hyaluronic acid, and elastin.
Japanese Patent Laid-Open No. 7-17844 Japanese Patent Laid-Open No. 7-145031 JP-A-5-279240 JP-A-6-128138 JP 2001-226286 A

  Hyaluronic acid is a polysaccharide in which glucuronic acid and acetylglucosamine are alternately bound, and has a characteristic of binding a large amount of water molecules. It is an important component that promotes cell migration and exists between cells in the tissue. It is also an important component of the synovial fluid and the vitreous body of the eye. Along with this, it has the function of making cells easier to divide and move. High molecular weight hyaluronic acid provides a moisturizing environment for the skin surface, but does not pass through the epidermis due to its high molecular weight.

  Collagen is an important protein that is present in the dermis layer below the epidermis and forms the skeleton of the tissue. Collagen molecules are those in which three polypeptides (protein molecules) form a triple helix structure.

  Arginine suppresses the expression of excessive cytokines (stimulating physiologically active substances) that are released along with the inflammatory reaction of tissues. Arginine is a typical basic amino acid and is a main component that produces NO (nitrogen monoxide), and this NO has a function of attacking tumor cells and cells accompanied by infection. In addition, arginine plays an important role in T-lymphocyte maturation. Thus, arginine is an important amino acid that promotes wound healing.

Ascorbic acid forms a semiquinone called monodehydroascorbic acid at an intermediate stage of oxidation, and this high reactivity causes a redox reaction. As a physiological action of ascorbic acid, the reaction of prolyl hydroxylase that changes proline in the collagen precursor to hydroxyproline is promoted together with iron during collagen synthesis. Thus, ascorbic acid is an important component that promotes collagen production to maintain the youthfulness of the skin.

  In the description of conventional products and the above-mentioned literature, hyaluronic acid and collagen are described as having a moisturizing effect, but the main reason is that both hyaluronic acid and collagen have high ability to bind water molecules. That's just a phenomenon on the surface of the skin. As for the true skin regeneration effect, it is apparent that improvement of the temporary moisturizing state of the skin surface is insufficient, and improvement of collagen regeneration of the upper dermis layer and improvement of the cell activity state of the epidermis are necessary.

  For this reason, the keratinocytes existing on the basement membrane at the boundary between the epidermis and the dermis will smoothly divide and move to the upper layer to become the stratum corneum, and the process of moving the dead cell debris away smoothly. Thus, the pigment component remaining in the epidermis is gradually eliminated, and it becomes possible to regenerate the skin with vigorous and moisturization.

  Accordingly, an object of the present invention is to provide a cosmetic liquid that can prepare an environment in which the movement of keratinocytes and fibroblasts can be promoted.

Thus, essence of the present invention, the hyaluronic acid and / or its acid derivatives, hyaluronic acid and / or derivatives thereof of the low molecular weight obtained by hydrolyzing hyaluronic acid and / or a derivative thereof, and a neutral collagen, It is obtained by heat-treating a mixed solution containing at least arginine.

  The collagen is preferably atelocollagen. Usually, at both ends of the triple helix structure of collagen, there is a portion called telopeptide that causes allergic reaction, and atelocollagen is obtained by removing this telopeptide.

  The cosmetic liquid includes a first preparation step of preparing a first hyaluronic acid aqueous solution by dissolving hyaluronic acid and / or a derivative thereof in distilled water; first dissolving hyaluronic acid and / or a derivative thereof in distilled water; A second preparation step of preparing a second hyaluronic acid aqueous solution by heat treatment; a third preparation step of preparing a collagen gel by neutralizing an acidic aqueous solution obtained by dissolving collagen in distilled water; arginine 4th preparation process which prepares basic aqueous solution obtained by melt | dissolving in distilled water to neutral arginine aqueous solution; to said 1st hyaluronic acid aqueous solution prepared at said 1st preparation process, said 2nd A first mixing step in which the second hyaluronic acid aqueous solution prepared in the preparation step is mixed to produce a first mixed solution; the first mixed solution produced in the first mixing step; Preparation A second mixing step in which the collagen gel prepared in step 2 is mixed to prepare a second mixed solution; the second mixed solution prepared in the second mixing step is prepared in the fourth preparing step. A third mixing step of preparing a third mixed solution by mixing the arginine aqueous solution prepared; and a heat treatment step of performing a second heat treatment on the third mixed solution prepared in the third mixing step It is. Also, a fourth mixing step is provided in which phenoxyethanol is added to the third mixed solution prepared in the third mixing step to prepare a fourth mixed solution, and in the heat treatment step, the fourth mixed solution is added to the fourth mixed solution. The second heat treatment may be performed.

  In the second preparation step, hyaluronic acid is hydrolyzed to reduce the molecular weight, and by mixing this, low molecular weight hyaluronic acid passes through the aging stratum corneum and into the deep layer of the epidermis and the upper layer of the dermis. Since the cell migration is promoted and the division and migration of epidermal cells can be improved, the removal of the aging stratum corneum can be promoted.

Moreover, by heat-treating the third or fourth liquid mixture, the triple helix structure peculiar to collagen collapses to form a chain polypeptide molecule (heat-denatured collagen), which can form a state entangled with hyaluronic acid molecules. . Thereby, the excessive slimy feeling seen in the aqueous solution of hyaluronic acid alone can be suppressed, and an appropriate smooth feeling can be expressed.

Furthermore, arginine with the property of enhancing immunity is mixed with hyaluronic acid aqueous solution, so that the basic amino acid arginine molecule is salt-bonded with the hyaluronic acid molecule having a carboxyl group. At this time, since the arginine molecule gradually dissociates from the hyaluronic acid molecule and penetrates into the skin, it is possible to obtain a continuous effect of arginine.

  Therefore, according to the said invention, since the synergistic effect of the hyaluronic acid which adjusted molecular size, the polypeptide derived from collagen, and arginine is expressed, the function as a basic cosmetics can fully be exhibited.

  Hereinafter, this embodiment will be described.

  The cosmetic liquid according to the present invention comprises hyaluronic acid and / or a derivative thereof, low molecular weight hyaluronic acid and / or a derivative thereof obtained by hydrolyzing hyaluronic acid and / or a derivative thereof, neutral collagen gel, and arginine. It is obtained by heat-treating a mixed solution containing at least. The collagen is atelocollagen from which the telopeptide causing allergic reaction has been removed.

  And the cosmetic liquid mentioned above is manufactured by the method shown by the 1st Example shown, for example in FIG.

  First, the hyaluronic acid aqueous solution (1) is prepared in the process of step 100 in FIG. In this step, sodium hyaluronate powder is dissolved in distilled water to prepare a neutral aqueous solution in the range of 0.5% to 2.0%. Specifically, 80 g to 280 g of sodium hyaluronate is prepared by dissolving in distilled water in the range of 14000 ml to 16000 ml.

  Next, in the step 110, a hyaluronic acid aqueous solution (2) is prepared. In this step, sodium hyaluronate powder is dissolved in distilled water to prepare a neutral aqueous solution of about 0.5% to 2.0%. Specifically, 11 g to 32 g of sodium hyaluronate is prepared by dissolving in 1600 ml to 2200 ml of distilled water. This aqueous hyaluronic acid solution (2) is hydrolyzed by the first heat treatment step shown in step 120 to become low molecular weight hyaluronic acid. Specifically, the heat treatment step of Step 120 is performed in a autoclave at a temperature of 105 to 121 ° C. for 20 minutes to 120 minutes. In the mixing step of Step 130, the heat-treated hyaluronic acid aqueous solution (2) is mixed with the hyaluronic acid aqueous solution (1) to prepare a mixed solution (1).

  Further, in step 140, an aqueous collagen solution is prepared. In this step, collagen powder is dissolved in distilled water to prepare an acidic aqueous solution of collagen, and this aqueous collagen solution is neutralized to form a gel. The mixture (2) is prepared by mixing with the mixture (1). Specifically, in the process of Step 140, 1.8 to 14 g of collagen powder is dissolved in distilled water in a range of 1400 ml to 1800 ml to prepare a 0.1% to 1.0% aqueous solution. In addition, since the basic amino acid is in the form of hydrochloride during the production process of collagen powder, this aqueous solution exhibits an acidity of pH 3 to 3.5. And by adjusting this aqueous solution to neutrality, collagen becomes insoluble and an aqueous solution will be in a gel state. Collagen becomes water-soluble in an acidic state of pH 4 or lower.

  In step 160, an arginine aqueous solution is prepared. In this step, a 2% to 10% arginine aqueous solution is prepared. Specifically, 5 to 15 g of arginine is dissolved in 150 to 250 ml of distilled water to prepare an arginine aqueous solution. Since this arginine aqueous solution shows basicity, it is adjusted to a neutral aqueous solution. And in the mixing process of step 170, it mixes with the said liquid mixture (2), and prepares a liquid mixture (3). At this time, the arginine molecule, which is a basic amino acid, forms a salt bond with a hyaluronic acid molecule having a carboxyl group. Thereby, when this cosmetic liquid is applied to the skin, an effect is obtained in which the arginine molecules gradually dissociate from the hyaluronic acid molecules and penetrate into the skin.

  The mixed solution (3) is subjected to a second heat treatment in the second heat treatment step of Step 200, and the triple helix structure of collagen collapses to become polypeptide molecules (heat-denatured collagen). It takes a state of being entangled with acid molecules. Thereby, the excessive slimy feeling seen in the aqueous solution of hyaluronic acid alone is suppressed, and an appropriate smooth feeling is expressed. The second heat treatment in step 200 is performed in a condition of a predetermined time between 5 minutes and 30 minutes at a temperature in the range of 105 ° C. to 121 ° C. in an autoclave.

  After that, the product is packed with a pump and a cap which are packed in a cleaned, dried and sterilized bottle, cleaned, dried and sterilized.

  In the second embodiment shown in FIG. 2, a mixing step of step 180 is added, and phenoxyethanol is added to the mixture (3) as a preservative at a predetermined ratio in the range of 0.1% to 1.0%. The mixed liquid (4) is prepared, and the mixed liquid (4) is heated in step 200 to be commercialized, which is different from the first embodiment. By adding a preservative, the quality can be maintained for a long time.

  Further, as shown in FIG. 1 and FIG. 2, Example 3 is a physiologically active substance such as ascorbic acid and its derivatives, which is preliminarily filtered and sterilized by providing the cosmetic liquid heat-treated in Step 200 with the mixing step in Step 210. It is characterized by adding an aqueous solution of

  Since the cosmetic liquid of the present invention effectively contains hyaluronic acid, heat-denatured collagen, arginine and ascorbic acid, it is effective as a basic cosmetic and can be used as aftercare for treatment of wounds and the like. Incidentally, collagen that retains the triple helix structure cannot pass through the epidermis, but the polypeptide molecule of heat-denatured collagen is easy to pass through the epidermis, and it is degraded by proteolytic enzymes present in the skin tissue. It becomes a small molecule that is easily received and moves into the dermal tissue. The collagen-derived polypeptide molecule that has been subjected to enzymatic degradation in this way expresses the effect of promoting migration of fibroblasts in the dermal tissue. It works as a so-called chemotactic factor.

It is explanatory drawing which showed the manufacturing method of the cosmetic liquid of this invention (Example 1). It is explanatory drawing which showed the manufacturing method of the cosmetic liquid of this invention (Example 2).

Claims (6)

Hyaluronic acid and / or its acid derivatives, hyaluronic acid and / or derivatives thereof of the low molecular weight obtained by hydrolyzing hyaluronic acid and / or a derivative thereof, and the neutral collagen, the mixture containing at least an arginine A essence obtained by heat treatment.   The cosmetic liquid according to claim 1, wherein the collagen is atelocollagen.   3. The cosmetic liquid according to claim 1 or 2, wherein the cosmetic liquid is obtained by mixing an aqueous solution of ascorbic acid and / or a derivative thereof sterilized by filtration in advance with the liquid mixture sterilized by the heat treatment. A first preparation step of preparing a first aqueous hyaluronic acid solution by dissolving hyaluronic acid and / or a derivative thereof in distilled water;
A second preparation step of preparing a second aqueous hyaluronic acid solution by dissolving hyaluronic acid and / or a derivative thereof in distilled water and performing a first heat treatment;
A third preparation step of preparing a collagen gel by neutralizing an acidic aqueous solution obtained by dissolving collagen in distilled water;
A fourth preparation step of preparing a basic aqueous solution obtained by dissolving arginine in distilled water into a neutral aqueous arginine solution;
First mixing for preparing the first mixed solution by mixing the first hyaluronic acid aqueous solution prepared in the first preparation step with the second hyaluronic acid aqueous solution prepared in the second preparation step Process;
A second mixing step of preparing a second mixed solution by mixing the collagen gel prepared in the third preparing step with the first mixed solution prepared in the first mixing step;
A third mixing step of preparing a third mixed solution by mixing the second mixed solution prepared in the second mixing step with the arginine aqueous solution prepared in the fourth preparing step; and The manufacturing method of the cosmetic liquid of Claim 1 or 2 which consists of a heat processing process which performs 2nd heat processing to the 3rd liquid mixture produced at the mixing process of this. A fourth mixing step is provided in which phenoxyethanol is added to the third mixed solution prepared in the third mixing step to prepare a fourth mixed solution;
The manufacturing method according to claim 4, wherein in the heat treatment step, a second heat treatment is performed on the fourth liquid mixture. A sterile mixed solution obtained by mixing an aqueous solution of ascorbic acid and / or a derivative thereof preliminarily filtered and sterilized with the sterile mixed solution obtained from the step of performing the second heat treatment on the third or fourth mixed solution. The method for producing a cosmetic liquid according to claim 4 or 5, wherein:

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