JP3665014B2 - Spectrofluorometer - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a spectrofluorometer, and more particularly to a spectrofluorometer suitable for measuring the fluorescence of trace substances in a solution.
[0002]
[Prior art]
In the prior art, a spectrofluorometer mainly includes a light source that generates excitation light, a spectroscope, a sample cell that houses a sample to be measured, a spectroscope for detecting fluorescence emitted by the sample, and optical detection It consists of a container.
[0003]
In a spectrofluorometer, light generated from a light source is converted into monochromatic light having a predetermined wavelength by an excitation-side spectrograph, and this monochromatic light is irradiated as excitation light onto a sample housed in a sample cell.
[0004]
Then, the fluorescence component is extracted from the emission light from the sample cell, that is, the emission light of the sample with the fluorescence side spectroscope, and the transition state of the component contained in the sample is detected by detecting the fluorescence with the photoelectric detector. The quantitative analysis of the components in the sample is performed from the intensity.
[0005]
Since the fluorescence emitted from the sample has no directivity, it is necessary to collect the fluorescence in order to efficiently introduce it into the fluorescence side spectroscope.
[0006]
In addition, since the excitation light is generally diffused when extracted from the excitation-side spectroscope, it is necessary to collect the excitation light in order to efficiently irradiate the sample.
[0007]
For this reason, in the prior art, as shown in FIG. 9, it is common to place a condensing lens between the sample cell, the fluorescence side spectroscope, and the excitation side spectroscope.
[0008]
That is, in FIG. 9, the light from the light source 12 is irradiated to the excitation side diffraction grating 14 through the slit 13. The light reflected from the excitation-side diffraction grating 14 is applied to the convex lens 15 through the slit 13, condensed, and applied to the sample in the flow cell (sample cell) 11.
[0009]
From the sample, reflected light is emitted with no directivity, but a part of the emitted light is condensed by the condenser lens 15 and only the light having the necessary wavelength is selected. After being irradiated and reflected on the fluorescence side diffraction grating 16, it is irradiated on the photodetector 17 through the slit 13.
[0010]
In this way, the condensing lens 15 is arranged between the sample cell 11 and the fluorescence side spectroscope 14 and between the sample cell 11 and the excitation side spectroscope 16, respectively, and effective use of irradiation light and reflected light is achieved. ing.
[0011]
[Problems to be solved by the invention]
However, the above prior art has the following problems because it uses a lens to collect fluorescence and excitation light.
[0012]
First, since the lens itself needs to be manufactured using an expensive material such as synthetic quartz glass, it is very expensive, which is an impediment to the price reduction of the spectrofluorometer.
[0013]
Secondly, it is necessary to accurately focus each lens on the center of the sample cell and the center of the slit of each spectroscope, and precise adjustment work occurs when the apparatus is assembled. In addition, a position error may occur due to a temperature change after the adjustment work.
[0014]
Third, since chromatic aberration due to the difference in refractive index with respect to the wavelength is unavoidable in the lens, the light collection efficiency may be insufficient depending on the wavelength of light.
[0015]
Due to the above-described problems, there is a technique related to a spectrofluorometer without the lens condensing system.
[0016]
For example, in the technique described in Japanese Patent Laid-Open No. Hei 6-273333, an incident slit of a fluorescence side spectroscope is formed in a sample cell, so that a sufficient angle of view (of the emitted light from the sample can be obtained even though the condensing system is omitted. This is an example in which the radiation angle of light that can be irradiated on the detector side is ensured.
[0017]
However, in the technique described in this publication, since the prospective angle is determined by the dimensions of the sample cell, it is difficult to greatly increase the prospective angle in the future, and it is difficult to improve the effective use of emitted light. It was.
[0018]
In addition, the technology described in the above publication can eliminate the excitation-side lens condensing system by making the exit slit of the excitation-side spectrometer into the sample cell and using a concave diffraction grating as the dispersion element. However, if the viewing angle is extremely wide, aberrations as a spherical mirror of a concave diffraction grating and image blur due to defocusing cannot be ignored, so there is a limit to expanding the viewing angle.
[0019]
On the other hand, conventionally, a method of increasing the fluorescence introduced into the fluorescence side spectroscope by using the end surface of the sample cell as a mirror surface has been widely used.
[0020]
Specifically, the end face directly facing the fluorescence extraction surface of the sample cell is a back mirror, so that fluorescence in the direction opposite to the daylighting window, which has been wasted in the past, can be introduced into the fluorescence side spectroscope.
[0021]
However, this forms a plane mirror in the sample cell. Therefore, the light that can be introduced into the fluorescence side spectroscope is limited to the component in the direction opposite to the daylighting window, and a large increase in the amount of light cannot be expected.
[0022]
On the other hand, an example of using a concave mirror is not a spectrofluorometer but a light scattering type particle detection device, which is described in JP-A-4-369464. In the technique described in this publication, light from the light source is irradiated onto the flow path in the flow cell, and a part of the light scattered in this flow path is on the side opposite to the photodetector with the flow path in between. Is reflected by the concave mirror arranged at the center of the channel.
[0023]
The light reflected by the concave mirror and converged on the flow path is diffused toward the photodetector together with other scattered light scattered from the flow path, but is collected in the light detector via the condenser lens. It is configured to be illuminated.
[0024]
Therefore, it is conceivable to apply the technique described in the above publication to a spectrofluorometer. In other words, the light reflected by the concave mirror and other scattered light scattered from the flow path are irradiated to the diffraction grating instead of the condenser lens, and the light reflected from the diffraction grating is collected on the detector. It is possible to make it.
[0025]
However, in the case of a spectrofluorometer, unlike the particle detector, a slit for selecting only light having a required wavelength out of the light scattered from the flow path is provided between the flow path and the diffraction grating. Need to be placed between.
[0026]
For this reason, when the technique described in the above publication is applied to a spectrofluorometer, a slit must be disposed between the diffraction grating and the flow path, so that the scattered light is allowed to pass through the slit. It is necessary to arrange the optical lens between the flow path and the slit.
[0027]
Therefore, a condensing lens for irradiating light to the flow path and a condensing lens for allowing scattered light from the flow path to pass through the slit are still required.
[0028]
An object of the present invention is to realize a fluorescence spectrophotometer capable of reducing the number of necessary condensing lenses, ensuring a prospective angle capable of meeting the demand for higher sensitivity, and simultaneously reducing cost and increasing sensitivity. It is.
[0029]
[Means for Solving the Problems]
In order to achieve the above object, the present invention is configured as follows.
(1) A sample cell in which a flow channel through which a measurement sample flows is formed , a light source unit that generates excitation light for irradiating the sample cell , and an excitation light side spectrometer that splits the excitation light from the light source unit A spectrofluorimetric spectrophotometer including: a fluorescence side spectroscope for spectroscopically analyzing fluorescence generated from the sample irradiated with light from the excitation light side spectroscope; and an optical detector for detecting light from the fluorescence side spectroscope in total, the sample cell, the first side of the sample cell, film functioning emission from the flow path as a fluorescent side entrance slit for emitting to the fluorescence side spectroscope is formed, the entrance slit There second side opposite the first side is formed, when the light emission from the flow path is reflected, is shaped to focused on the center of the entrance slit, the second side A film is formed in which the cell side is a mirror .
[0030]
(2) Preferably, in the above (1) , the sample cell is an excitation light side exit slit for allowing light from the excitation light side spectrometer to enter the sample cell on the third side surface of the sample cell. functional film is formed, a fourth side surface opposite to the third aspect of the exit slit is formed, light emitted from the exit slit is formed in a shape such that focused on the flow path, A film having the cell side as a mirror is formed on the fourth side surface.
[0031]
(3) In addition, preferably, in the above (1), said first side said entrance slit is formed is formed in a cylindrical surface shape centered axis the flow path within the sample cell.
[0032]
(4) Further, preferably, in the above (1), said first side said entrance slit is formed, is formed in a spherical shape centered on the flow path within the sample cell.
[0034]
( 5 ) The excitation light generated from the light source part is dispersed by the excitation light side spectroscope and irradiated to the measurement sample, and the fluorescence generated from the measurement sample is spectrally separated by the fluorescence side spectroscope. In a sample cell used in a spectrofluorophotometer for detecting light and having a flow channel through which the measurement sample flows , light emitted from the flow channel is emitted from the flow channel to the first side surface of the sample cell . A film functioning as a fluorescent side entrance slit for emitting light is formed, and the second side surface opposite to the first side surface on which the entrance slit is formed is A film is formed so as to be condensed at the center of the entrance slit, and a film with the cell side serving as a mirror is formed on the second side surface .
[0035]
(6) Preferably, in the above (5), to a third aspect of the sample cell, film functioning light from the excitation light side spectroscope as an excitation light side exit slit for causing incident on the sample cell is formed is, the fourth side surface opposite to the third aspect of the exit slit is formed, light emitted from the exit slit is formed in a shape such that focused on the flow path, the fourth aspect of A film is formed in which the cell side is a mirror.
[0036]
(7) In addition, preferably, in the above (5), said first side said entrance slit is formed is formed in a cylindrical surface shape centered axis the flow path within the sample cell.
[0037]
(8) Further, preferably, in the above (5), said first side said entrance slit is formed, is formed in a spherical shape centered on the flow path within the sample cell.
[0039]
Since the backside boundary, the part where the sample is located (light emission point), and the slit are integrated into the sample cell, the light emission point and the back mirror can be brought close to each other, and the angle of view is made larger than before. And the sensitivity of the photometer can be improved by increasing the amount of light introduced into the fluorescence spectrometer.
[0040]
In addition, when the slit is configured separately from the sample cell, a condensing lens is required between the light emitting point and the slit, but the slit is integrated with the sample cell. The condenser lens can be omitted, and the cost can be reduced.
[0041]
DETAILED DESCRIPTION OF THE INVENTION
Embodiments of the present invention will be described below with reference to the accompanying drawings.
FIG. 1 is a schematic cross-sectional view of a flow cell (sample cell) 11 in a spectrofluorophotometer according to the first embodiment of the present invention, and FIG. 2 is a spectrofluorophotometer according to the first embodiment of the present invention. FIG. The example shown in FIGS. 1 and 2 is an example in which the present invention is applied to a spectrofluorometer for liquid chromatography.
[0042]
In FIG. 1, this flow cell 11 has four side surfaces extending in the longitudinal direction, and a cylindrical sample channel 4 is formed in the central portion of the flow cell main body 1 so that the sample passes in the longitudinal direction. ing.
[0043]
For example, a slit 2 is formed on one side surface of the flow cell main body 1 by an Inconel vapor deposition film, and the slit 2 is opposed to the fluorescence side diffraction grating (fluorescence side spectroscope) 16 as shown in FIG. 11 is disposed (the flow cell main body 1 is a state in which the slit 2 and the rear-view mirror 3 described later are not formed, and the flow cell 11 is formed in which the slits 2 and the like are formed).
[0044]
In addition, the flow cell 11 has a side face directly facing the formation surface of the slit 2 formed into an elliptic cylindrical surface having two focal points at the center point of the flow path 4 and the center point of the slit, and on the back surface (the inner surface side of the flow cell 1). By performing Inconel vapor deposition, an elliptical cylindrical surface back mirror (elliptical cylindrical surface focusing mirror) 3 is formed. The angle at which the center point of the sample channel 4 looks at the back mirror 3 is the prospective angle.
[0045]
In the flow cell 11 configured as described above, when the sample channel 4 is irradiated with excitation light from one side of the side surface other than the side surface where the back mirror 3 is formed and the side surface where the slit 2 is formed, the sample channel 4 The fluorescence generated from the inner sample is emitted toward the entire periphery of the flow path 4.
[0046]
Of the fluorescence emitted toward the entire circumference of the flow path 4, the light that looks into the back mirror 3 that is an elliptic cylindrical surface is condensed at the center of the entrance slit 2 due to the optical characteristics of the elliptic cylindrical surface, The light that has passed through the slit is introduced into the fluorescent side diffraction grating 16.
[0047]
That is, as shown in FIG. 2, the light reflected from the back mirror 3 becomes light having a required wavelength by the entrance slit 2 formed in the flow cell 11, and thus the light emitted from the flow cell 11. It is not necessary to collect the light with a condenser lens and pass through the slit, and the condenser lens between the flow cell 11 and the fluorescent diffraction grating 16 can be omitted.
[0048]
Moreover, in the flow cell 11 shown in FIG. 1, since the condensing mirror (back surface mirror 3) is formed on the side surface of the flow cell, the light emitting point (the center point of the flow path 4) and the condensing mirror are close to each other. In this embodiment, the prospective angle can be made wider than that of the conventional angle. This is more than twice the value of a conventional spectrofluorometer.
[0049]
Therefore, the amount of fluorescence introduced into the fluorescence side spectroscope 17 is also doubled or more, and the detection sensitivity of the apparatus can be improved.
[0050]
In addition, since the light emitting point, the condensing mirror 3 and the entrance slit (incident slit of the fluorescence side spectroscope) 2 are built in the flow cell 11 alone, the positional relationship between them is set with the dimensional accuracy required when manufacturing the cell. In this case, optical adjustment of the condensing system is unnecessary, which is advantageous in terms of assembly cost.
[0051]
Further, in the prior art, after adjusting the positional relationship among the fluorescent-side condenser lens, the flow cell 11 and the slit, the positional relationship may be different from that initially set due to the difference in the respective thermal expansion coefficients. However, in the first embodiment of the present invention, since the light emitting point, the condensing mirror, and the entrance slit are formed in the flow cell 11 alone, the fluorescent side condensing lens is unnecessary and the positional relationship is initial. It is unlikely to be different from the one set to.
[0052]
Further, since a mirror is used for condensing, there is no problem of chromatic aberration in the case of lens condensing.
[0053]
In addition, since all of the light condensing system is built in the cell 11 made of quartz glass, the thermal expansion due to the temperature change of the apparatus is extremely small, and the light of the optical system that has conventionally caused the temperature drift. Axial distortion can be eliminated for this part.
[0054]
As described above, according to the first embodiment of the present invention, the number of necessary condensing lenses is reduced, the prospective angle that can meet the demand for higher sensitivity is increased, and low cost and high sensitivity are achieved at the same time. A possible fluorescence spectrophotometer can be realized.
[0055]
In the example shown in FIG. 1, the condensing mirror (back mirror 3) of the sample cell 1 is an elliptical cylindrical surface, but it can be replaced with a cylindrical mirror centered on the center point of the slit 2. .
[0056]
In this case, the aberration becomes larger than the normal case due to the size of the prospective angle. However, if the performance that can be practically used is obtained, the processing is easier than the aspherical mirror and the cost is advantageous.
[0057]
In addition, the slit width of the slit 2 can be set to a dimension that can be appropriately designed from the resolution of the applied spectrophotometer.
[0058]
FIG. 3 is an overall schematic configuration diagram of a spectrofluorometer which is the second embodiment of the present invention. In the second embodiment, the configuration of the flow cell itself is the same as that of the first embodiment.
[0059]
The difference between the first embodiment and the second embodiment is that the flow cell shown in FIG. 1 is arranged so that the slit 2 faces the fluorescent diffraction grating 16, but the second In the embodiment, the slit 2 is disposed so as to face the excitation light side diffraction grating (excitation light side spectroscope) 14.
[0060]
That is, in FIG. 3, the light from the light source 12 is irradiated to the excitation side diffraction grating 14 through the slit 13. Then, the light reflected from the excitation light side diffraction grating 14 is irradiated to the sample in the flow path 4 through the slit 2 formed in the flow cell 11 (in this case, the exit slit of the excitation light side spectrometer). At the same time, the light is reflected by the back mirror 3, collected, and irradiated on the sample.
[0061]
Then, the reflected light from the sample in the flow cell 11 is collected by the condenser lens 15, irradiated to the fluorescent side diffraction grating 16 through the slit 13, reflected, and then the photodetector through the slit 13. 17 is irradiated.
[0062]
According to the second embodiment of the present invention, since the light emitting point, the condensing mirror 3 and the entrance slit 2 are formed in the flow cell 11 alone, the light having the required wavelength is transmitted through the slit 2 by the slit 2. Therefore, the condenser lens between the flow cell 11 and the excitation-side diffraction grating 14 can be omitted.
[0063]
FIG. 4 is a schematic cross-sectional view of the flow cell 11 in the spectrofluorometer that is the third embodiment of the present invention, and FIG. 5 is an overall schematic diagram of the spectrofluorometer that is the third embodiment of the present invention. It is a block diagram.
[0064]
In FIG. 4, the flow cell 11 has four side surfaces extending in the longitudinal direction, and a sample channel 4 is formed in the central portion of the flow cell main body 1 so that the sample passes in the longitudinal direction.
[0065]
On the two side surfaces of the flow cell body 1 that are not opposite to each other (adjacent to each other), for example, slits 2 are formed by an Inconel vapor deposition film, and the slits 2 on one side surface, as shown in FIG. The flow cell 11 is arranged so that the slit 2 on the other side faces the excitation side diffraction grating 16.
[0066]
In addition, the flow cell 11 is formed such that a side surface directly opposite to each side surface where the slit 2 is formed is formed into an elliptic cylindrical surface having two focal points at the center point of the flow path 4 and the slit center point. The back mirror 3 having an elliptical cylindrical surface is formed by performing Inconel vapor deposition on the inner surface side of the surface. The angle at which the center point of the sample channel 4 looks at the back mirror 3 is the prospective angle.
[0067]
Next, in FIG. 5, the light from the light source 12 is irradiated to the excitation side diffraction grating 14 through the slit 13. And the light reflected from this excitation side diffraction grating 14 is irradiated to the sample in the flow path 4 through the slit 2 formed in the flow cell 11, and is reflected and condensed by the back mirror 3. The sample is irradiated.
[0068]
The fluorescence emitted from the sample is reflected by the back mirror 3 which is an elliptical cylindrical surface, collected at the center of the entrance slit 2, and the light passing through the slit 2 is introduced into the fluorescence side diffraction grating 16.
[0069]
The light irradiated on the fluorescent side diffraction grating 16 is reflected by the fluorescent side diffraction grating 16 and then irradiated on the photodetector 17 through the slit 13.
[0070]
Therefore, according to the third embodiment of the present invention, the condenser lens between the flow cell 11 and the excitation-side diffraction grating 14 and the condenser lens between the flow cell 11 and the fluorescence-side diffraction grating 16 are omitted. be able to.
[0071]
FIG. 6 is a schematic cross-sectional view of the flow cell 11 in the spectrofluorometer according to the fourth embodiment of the present invention. In the fourth embodiment, the shape of the flow path 4 is a prismatic shape in the first embodiment, and other configurations are the same as those in the first embodiment.
Also in the fourth embodiment, the same effect as in the first embodiment can be obtained.
[0072]
FIG. 7 is a schematic cross-sectional view of the flow cell 11 in the spectrofluorometer that is the fifth embodiment of the present invention.
[0073]
In the example shown in FIG. 7, the cylindrical mirror 6 having the central axis of the flow cell 11 as the axis is formed on the side surface of the flow cell 11 shown in FIG. This is an example.
[0074]
In the example shown in FIG. 1, only the component that directly hits the collector mirror 3 among the fluorescence emitted from the sample emission center that is the center of the flow path 4 is collected, and the other portions are not used. In the fifth embodiment of the present invention shown in FIG. 7, all or part of the light that does not directly hit the condenser mirror 3 is introduced into the condenser mirror 3 by returning the light in the opposite direction by the cylindrical mirror 6. Accordingly, the amount of fluorescence introduced into the fluorescence side diffraction grating 16 can be further increased.
[0075]
Therefore, according to the fifth embodiment, the same effects as those of the first embodiment can be obtained, and the amount of fluorescence introduced into the fluorescence side diffraction grating 16 can be further increased.
[0076]
FIG. 8 is a sixth embodiment of the present invention, and is an example in which the cylindrical mirror 6 in the example shown in FIG. 7 is replaced with a spherical mirror 7 centering on the emission center of the sample cell 11, and other configurations are as follows. It is the same as FIG.
[0077]
In the sixth embodiment, the same effect as that of the example shown in FIG. 7 can be obtained.
[0078]
【The invention's effect】
According to the present invention, a fluorescent spectrophotometer capable of reducing the number of necessary condensing lenses, ensuring a prospective angle capable of responding to a demand for high sensitivity, and simultaneously realizing cost reduction and high sensitivity can be realized. Can do.
[0079]
In other words, since the collector mirror (backside boundary), the light emitting point, and the slit are integrated with the flow cell, the light emitting point and the collector mirror can be brought close to each other, and the prospective angle should be larger than before. And the sensitivity of the apparatus can be improved by increasing the amount of light introduced into the fluorescence side spectroscope.
[0080]
In addition, when the slit is configured separately from the flow cell, a condensing lens is required between the light emitting point and the slit. However, since the slit is integrated with the flow cell, the light condensing between them is integrated. The lens can be omitted, and the cost can be reduced.
[0081]
In addition, since the light collecting system is built in the flow cell which is one part, the optical adjustment process at the time of assembling the fluorescence spectrophotometer can be reduced, and the assembling cost can be reduced.
[0082]
Further, since the mirror is focused, there is no problem of chromatic aberration in the case of lens focusing, and the same focusing efficiency can be realized at all wavelengths.
[0083]
Further, since the light condensing system is entirely built in the flow cell made of quartz glass, there is almost no distortion of the optical axis due to thermal expansion, and the temperature drift of the apparatus can be improved.
[0084]
Further, when the present invention is applied to the excitation light collection, the excitation light introduced from the slit is collected at the center of the cell, so that the sample can be irradiated with the excitation light very efficiently.
[0085]
In addition, when a cylindrical mirror or spherical mirror is added to the side facing the side on which the collector mirror is formed, all or part of the light that does not directly hit the collector mirror is also collected by the cylindrical mirror or spherical mirror. The amount of fluorescent light introduced into the fluorescent side diffraction grating can be increased.
[Brief description of the drawings]
FIG. 1 is a schematic cross-sectional view of a flow cell in a spectrofluorometer according to a first embodiment of the present invention.
FIG. 2 is an overall schematic configuration diagram of a spectrofluorometer which is a first embodiment of the present invention.
FIG. 3 is an overall schematic configuration diagram of a spectrofluorometer according to a second embodiment of the present invention.
FIG. 4 is a schematic cross-sectional view of a flow cell in a spectrofluorometer according to a third embodiment of the present invention.
FIG. 5 is an overall schematic configuration diagram of a spectrofluorometer according to a third embodiment of the present invention.
FIG. 6 is a schematic sectional view of a flow cell in a spectrofluorometer according to a fourth embodiment of the present invention.
FIG. 7 is a schematic cross-sectional view of a flow cell in a spectrofluorometer that is a fifth embodiment of the present invention.
FIG. 8 is a schematic cross-sectional view of a flow cell in a spectrofluorometer that is a sixth embodiment of the present invention.
FIG. 9 is an overall schematic configuration diagram of a spectrofluorometer in the prior art.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 1 Flow cell main body 2 Deposition film slit 3 Elliptical cylindrical surface condensing mirror 4 Sample flow path 5 Focal point of elliptic cylindrical surface condensing mirror 6 Deposition film slit / cylindrical mirror 7 Deposition film slit / spherical mirror 11 Flow cell 12 Light source 13 Slit 14 Excitation Side diffraction grating 15 Condensing lens 16 Fluorescence side diffraction grating 17 Photodetector

Claims (8)

A sample cell in which a flow path for a measurement sample is formed , a light source unit that generates excitation light for irradiating the sample cell , an excitation light side spectrometer that splits excitation light from the light source unit, and In a spectrofluorometer having a fluorescence side spectrometer that splits fluorescence generated from the sample irradiated with light from the excitation light side spectrometer and an optical detector that detects light from the fluorescence side spectrometer,
The sample cell is
The first side of the sample cell, film functioning emission from the flow path as a fluorescent side entrance slit for emitting to the fluorescence side spectroscope is formed,
The second side surface opposite to the first side surface on which the incident slit is formed is formed in a shape that condenses at the center of the incident slit when light emitted from the flow path is reflected . 2. A spectrofluorometer characterized in that a film having a mirror on the cell side is formed on the side surface of 2 . The spectrofluorometer according to claim 1 , wherein
In the sample cell, a film functioning as an excitation light side exit slit for allowing light from the excitation light side spectroscope to enter the sample cell is formed on the third side surface of the sample cell ,
Fourth side opposite the third side the exit slit is formed, light emitted from the exit slit is formed in a shape such that focused on the flow path, above the fourth side A spectrofluorometer characterized in that a film is formed on the cell side as a mirror. 2. The spectrofluorometer according to claim 1, wherein the first side surface on which the entrance slit is formed is formed in a cylindrical surface shape having a flow path in the sample cell as a central axis. Fluorometer. In spectrofluorometer according to claim 1, wherein said entrance slit is formed above first aspect, spectrofluorometer, characterized in that it is formed in a spherical shape centered on the flow path within the sample cell Total. The excitation light generated from the light source is dispersed by the excitation light side spectrometer and irradiated to the measurement sample, and the fluorescence generated from the measurement sample is dispersed by the fluorescence side spectrometer to detect the dispersed light. In a sample cell in which a flow path through which the measurement sample flows is formed ,
On the first side surface of the sample cell, a film functioning as a fluorescence side entrance slit for emitting light from the flow path to the fluorescence side spectrometer is formed,
The second side surface opposite to the first side surface on which the incident slit is formed is formed in a shape that condenses at the center of the incident slit when light emitted from the flow path is reflected . 2. A sample cell, wherein a film having the cell side as a mirror is formed on a side surface of the sample cell. The sample cell of claim 5 ,
On the third side surface of the sample cell, a film functioning as an excitation light side exit slit for allowing light from the excitation light side spectroscope to enter the sample cell is formed,
The fourth side surface facing the third side surface where the exit slit is formed is formed in a shape that condenses the light emitted from the exit slit in the flow path, and the fourth side surface A sample cell in which a film that forms a mirror on the cell side is formed . 6. The sample cell according to claim 5, wherein the first side surface on which the entrance slit is formed is formed in a cylindrical surface shape having a flow path in the sample cell as a central axis. In sample cell according to claim 5, wherein said entrance slit is formed above first aspect, a sample cell, characterized in that formed in spherical surface around the flow path in the sample cell.

Images