JP3565468B2 - Photosynthetic culture device - Google Patents

Photosynthetic culture device Download PDF

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Publication number
JP3565468B2
JP3565468B2 JP01452197A JP1452197A JP3565468B2 JP 3565468 B2 JP3565468 B2 JP 3565468B2 JP 01452197 A JP01452197 A JP 01452197A JP 1452197 A JP1452197 A JP 1452197A JP 3565468 B2 JP3565468 B2 JP 3565468B2
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Japan
Prior art keywords
light
culture
photosynthetic
sunlight
culture solution
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JPH10191956A (en
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健治 山村
文一 末広
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Research Institute of Innovative Technology for Earth
Sumitomo Heavy Industries Ltd
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Research Institute of Innovative Technology for Earth
Sumitomo Heavy Industries Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/08Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors

Description

【0001】
【発明の属する技術分野】
本発明は、光合成によりCOを生物的に固定し、大気中のCOを低減するための、藻類などの光合成微生物の培養装置(以下、光合成培養装置)に関し、太陽光あるいは人工光を効率良く利用して、培養液中の光合成微生物に光を供給し、該光合成微生物を効率的に増殖させる装置に関する。
【0002】
【従来の技術】
化石燃料の燃焼などによる二酸化炭素(以下、CO)の排出量は日本国内で年間約12億トンといわれ膨大である。近年、地球温暖化の原因の一つとして大気中におけるCOの増加が問題となっており、COの低減技術が要望されている。このCOを低減する種々の技術が研究開発されているが、実用化を図るにはエネルギーの消費量を最小限に抑え、かつ大量のCOを低減できる技術が必要である。
【0003】
光合成により生物的にCOを固定し低減する技術は、エネルギーとして太陽光が利用でき、しかも環境を汚染するような副産物を生じないため環境調和型の技術として期待されている。太陽光を利用する光合成微生物の培養法としては、太陽照射下の屋外で培養するオープンポンド式が太陽光を直接利用する方式としてよく知られている。しかし、この方式において大量培養する場合、光の透過距離が液面から僅かなため液深を大きく取ることができず、結果として広大な敷地面積を必要とする大きな欠点を有する。
【0004】
一方、光合成を利用した生物的CO固定には太陽光あるいは人工光を高効率に利用でき、液深が大きくとれる光合成培養装置として次に示す培養技術などが知られている。例えば、特開昭50−157581号公報のように光を透過するガラスなどの材質を外壁として用いた培養槽の外部から光供給を行う外部照射型の光合成培養装置や、特開昭57−102181号公報、特開昭61−139382号公報などのように外部の光源から光ファイバーなどの光伝送媒体により光を培養槽内に導いて光供給を行う内部照射型の光合成培養装置などがある。また、特開平6−165669号公報のように、培養槽の深さ方向に太陽光を直接照射する培養区域と伝送した光を照射する培養区域を設けることにより、深さ方向を考慮した光を効率的に利用した内部照射型の光合成培養装置がある。
【0005】
【発明が解決しようとする課題】
前記従来の外部照射型の光合成培養装置において、その照射面積を大きくするにはガラス等の光透過性の材質でできた槽壁部などを大きくする必要があり、構造上、強度上の面から培養槽の大型化は極めて困難であるという問題を有する。また、この外部照射型の光合成培養装置では、光合成微生物の濃度が高くなると、光の到達する距離が短くなり、供給される光量が不足して光合成微生物の増殖が抑えられるという問題を有している。
【0006】
一方、前記内部照射型の光合成培養装置において、光放射面積を大きくするには槽内部に取り付ける光散乱体の数を増やすことで可能であり、前記外部照射型のスケールアップや培養槽の光透過距離の問題を回避できる。
【0007】
しかしながら、前記内部照射型の光合成培養装置は基本的に太陽光あるいは人工光を集光して培養槽内部へ伝送する必要がある。内部照射型の光合成培養装置において、太陽光を利用する場合、集光するための装置としては、集光レンズによるものと集光ミラーによるものがあるが、いずれも地表に到達する太陽光のうち平行光線として入射する直達光しか利用できない。大気や雲の反射によって生ずる散乱光については入射させて利用することができないという太陽光の利用効率上問題がある。
【0008】
また、前記内部照射型の光合成培養装置に供給するための光を集光するには、直達光に対し集光器の受光面が一定の角度(例えば、垂直)を保つ必要があり、そのために太陽を追尾しなければならないという面倒がある。さらに、これら集光装置で太陽光(直達光)を100%集光し、伝送することは現状では困難である。また人工光を利用する場合でも、基本的には、太陽光と同様に、集光して培養槽内へ伝送し、放射する必要があり、したがって、集光器、光伝送媒体、及び光放射媒体が必須となる。
【0009】
このように前記従来の集光機能を有する内部照射型の光合成培養装置では、光利用効率が低く、集光、伝送、放射からなる光利用システムに大きなコストがかかることが問題である。
【0010】
上記した問題点に鑑み、本発明は、光の利用効率が高く、簡便な装置で単位受光面積あたりの光合成能、つまりCO固定能、の高い光合成培養装置を提供し、地球温暖化防止に寄与することを目的とする。
【0011】
【課題を解決するための手段】
上記の問題点を解決するために、本発明は、光合成微生物を培養するための光合成培養装置において、培養槽、該培養槽内に収容されている培養液、及び光を該培養液内部に供給するための該培養液に浸漬されている集光レンズを具備しない平板状光散乱体を有し、該光合成培養装置の上部は、太陽光の受光部を形成し、該受光部は、太陽光の直達光及び散乱光が入射可能な培養液面と、太陽光の直達光及び散乱光が入射可能な該光散乱体の光入射端面より構成されることを特徴とする光合成培養装置である。
【0012】
前記光合成培養装置において、光散乱体は、入射した光を光入射端面以外の表面から散乱放射できる機能を有する平板状の光散乱体とすることができる。前記光合成培養装置において、光散乱体は、光入射端面に対して反対側の端部が培養槽底部に位置するように垂直に配置され、且つ同一平面上に間隔を空けて複数列配置されていることを特徴とする。
【0013】
前記光合成培養装置において、二酸化炭素を含むガスを培養槽底部から気泡として吐出可能で、吐出される気泡が複数列の光散乱体間及び該光散乱体の表面上で上昇することができ、該気泡の上昇により培養液の上下方向の循環流動を生じさせ、該循環流動により培養液面の受光部付近の培養液が常時更新されることが可能な機能を有し、通気配管を培養槽底部で且つ該光散乱体の近傍に設けることができる。
【0014】
本発明の光合成培養装置に使用される光散乱体は、光の入射端面は集光レンズなどを具備しない構造であるため直達光と散乱光のどちらも入射可能である。しかも、受光部は、培養液面と、該培養液面より突出した一定の間隔を保った複数列の光散乱体の光入射端面とから構成されており、該受光部のいずれの面も直達光と散乱光のどちらも入射可能であるため、太陽光及び人工光のいずれも効率良く入射利用することができる。
【0015】
本発明の光合成培養装置に使用される光散乱体は、入射した光を光入射端面以外の全ての表面において散乱放射することが可能であるため、光散乱体は入射した太陽光或いは人工光を培養槽内の培養液中に効率よく供給することができる。本発明の光合成培養装置は、液面からの光供給ができない槽深部に光散乱体が浸漬されているので、培養槽のどの部位においても光供給が可能であり、従来のオープンポンドなどの直接受光型の光合成培養装置に比較して単位受光面積あたりの光合成能が高い。
【0016】
本発明の光合成培養装置においては、培養槽の底部よりCOを含むガスを気泡として吐出し、気泡の上昇によるリフト効果で培養液の上下方向の流動を生じるように攪拌混合を行うことができるので、光供給量が最も多い培養槽上部液面付近の培養液の更新を積極的に促進することができ、したがって、光合成微生物の培養を促進し、単位受光面積あたりの光合成能、即ち、CO固定能を高める。
【0017】
また平板状光散乱体の平板表面で相対する他の平板表面により仕切られたような空間となり、この空間で培養液の上下循環流動部分が形成されるので、該培養液の上下循環流動がさらに円滑となるように光散乱体の配置を工夫して前記の培養液更新効果を高めることもできる。
【0018】
本発明の光合成培養装置は、培養槽内に一定間隔に保った各々独立した複数列の光散乱体を有し、培養液内に均一な光供給密度で光を供給することができるため、培養のスケールアップをする際においても、容易に光供給密度を一定にすることができ、光合成培養装置の大型化が可能である。
【0019】
【発明の実施の形態】
本発明の実施の態様を図面に基づき説明する。図1は、本発明による光合成培養装置の全体概要の一例を示した図である。また図2は図1のA〜A断面である。図中1は培養槽、2は培養槽1内に収容された培養液8に浸漬されている平板状光散乱体である。平板状光散乱体2は、その一方の端部に光入射端面3を有し、該光入射端面3から入った光を光入射端面3以外の平板状光散乱体2の表面全体から放射できる構造体である。
【0020】
本発明の光合成培養装置における平板状光散乱体2には、例えば、特開平8−262232号公報に記載の光散乱体が利用できる。該公報に記載の光散乱体は、光散乱面を形成し、端面から入射された光を透明板全体から均一に散乱できる構成となったものである。
【0021】
本発明の光合成培養装置における平板状光散乱体2は、一方の端面がそのまま光入射端面となっており、集光機能を持たせない構造であるので、直達光及び散乱光の両方を同時に入射できる機能を有する。平板状光散乱体2は縦長の形状をしており、該縦長方向が培養槽1の深さ方向になるように同一平面上に一定の間隔を持って複数列、培養槽1内に垂直に配置されており、且つ光入射端面3に対して反対側の端部が培養槽内底部に位置するように配置されている。
【0022】
しかしながら、本発明の光合成培養装置における平板状光散乱体2の形状は縦長形状に限られるものではない。また、一端面から光を入射でき表面全体から入射光を散乱放射できる構造体であれば、前記特開平8−262232号公報に記載の光散乱体に限られるものではない。
【0023】
4は培養液面であり、平板状光散乱体2の光入射端面3は、直接的に光を受光するために培養液面4より上部に突出するように配置される。5は太陽光あるいは人工光である。6は光合成に必要なCOを供給するための通気用配管であり、該通気用配管6は、COを含むガスを吐出するためのスパージャ10に通じている。該スパージャ10は、ガスの吐出に適した微細な透口を多数有し、培養槽1内の底部に、且つ各平板状光散乱体2付近に配置される。
【0024】
培養液面4及び平板状光散乱体2の光入射端面3に太陽光又は人工光5を供給する。供給する光は直達光及び散乱光の何れでも利用可能である。平板状光散乱体2の光入射端面3から入った光は、平板状光散乱体2の表面全体から散乱放射され、培養槽1の深部に至るまで供給される。
【0025】
通気用配管6を通してCOを含む空気7が、図2に示すスパージャ10によって培養槽1内の底部より上部に向けて吐出される。吐出されたCOを含む空気7は光合成微生物にCOを供給するとともに、ガスリフト効果により図2中の矢印で示す培養液流動パターン11のように培養液の上下方向の循環流動を生じさせる。この循環流動は、光供給量の多い培養液面4付近の培養液8の更新を促進し、光合成微生物に対して効率的な光合成を行なわせることができる。本光合成培養装置の上部は開放されていてもよく、また必要に応じて、例えば、ガス管理、コンタミネーション防止等の目的のために光透過性の蓋を設けてもよい。
【0026】
平板状光散乱体2は、図1及び図2に示すように、その平板表面で一部隙間を持った壁が形成されるように同一平面上に複数列配置され、各平板状光散乱体2の壁の間の培養槽1内の底部から、COを含むガス7を吐出することにより、平板状光散乱体2で形成された壁の方向に、培養液8の二次元的でスムーズな上下循環流動が生じる。
【0027】
図1中8は供給される培養液であり、培養槽1内の光合成微生物に微量栄養源などを供給する。また9は抜き出し培養液であり、この抜き出し培養液9からCO固定産物である光合成微生物などを分離回収して有効利用することができる。
【0028】
【発明の効果】
本発明の光合成培養装置によれば、培養液面から培養液に太陽光あるいは人工光を直接供給できるとともに、光散乱体の光入射端部から培養槽深部にまで光供給ができるため、単位受光面積あたりの光合成能が高い。
【0029】
また、本発明の光合成培養装置によれば、直達光と散乱光の両方が利用できるので、太陽光の利用効率が高い。特に、内部照射用の光散乱体の光入射端面も直達光、散乱光とも入射可能なので、従来の直達光のみ受光の内部照射装置に比べて太陽光利用効率が高い。
【0030】
また、本発明の光合成培養装置によれば、太陽光あるいは人工光が直接照射される培養液面付近の受光量の大きい培養液の更新が図れるように培養液を循環流動させる機能を有するので、光合成を効率よく行わせることができる。
【0031】
また、本発明の光合成培養装置によれば、光散乱体には集光装置、光伝送用ファイバーなどを具備しないため、従来の集光型の光合成培養装置に比較して建設費が大幅に削減できる効果を有する。
【0032】
本発明による光合成培養装置を用いることにより、地球温暖化の原因物質の一つであるCOを効率よく生物的に固定でき、地球環境保全に寄与できる。
【図面の簡単な説明】
【図1】本発明の光合成培養装置の全体概要を示す。
【図2】図1のA〜A断面を示す。
【符号の説明】
1 培養槽
2 平板状光散乱体
3 光入射端面
4 培養液面
5 太陽光又は人工光
6 通気用配管
7 COを含む空気
8 培養液
9 抜き出し培養液
10 スパージャ
11 培養液流動パターン
[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention relates to an apparatus for culturing photosynthetic microorganisms such as algae (hereinafter referred to as a photosynthetic culture apparatus) for biologically fixing CO 2 by photosynthesis and reducing CO 2 in the atmosphere. BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an apparatus which, when used well, supplies light to photosynthetic microorganisms in a culture solution to efficiently grow the photosynthetic microorganisms.
[0002]
[Prior art]
Emissions of carbon dioxide (hereinafter referred to as CO 2 ) due to combustion of fossil fuels are enormous in Japan, amounting to about 1.2 billion tons per year. In recent years, as one of the causes of global warming, an increase in CO 2 in the atmosphere has become a problem, and a technology for reducing CO 2 has been demanded. Various technologies for reducing this CO 2 have been researched and developed, but for practical use, a technology capable of minimizing energy consumption and reducing a large amount of CO 2 is required.
[0003]
The technology for biologically fixing and reducing CO 2 by photosynthesis is expected to be an environmentally friendly technology because sunlight can be used as energy and no by-products that pollute the environment are produced. As a method of cultivating photosynthetic microorganisms using sunlight, an open pond method of cultivating outdoors under sun irradiation is well known as a method of directly using sunlight. However, when a large amount of culture is performed in this method, the light transmission distance is short from the liquid level, so that a large liquid depth cannot be obtained, and as a result, there is a large disadvantage that a large site area is required.
[0004]
On the other hand, for biological CO 2 fixation using photosynthesis, the following culturing techniques and the like are known as photosynthetic culturing devices capable of utilizing sunlight or artificial light with high efficiency and having a large liquid depth. For example, as disclosed in JP-A-50-157581, an external irradiation type photosynthetic culture apparatus that supplies light from the outside of a culture tank using a material such as glass that transmits light as an outer wall, and JP-A-57-102181. There is an internal irradiation type photosynthetic culture apparatus which supplies light by guiding light into a culture tank from an external light source by an optical transmission medium such as an optical fiber as disclosed in Japanese Patent Application Laid-Open No. 61-139382. Further, as disclosed in Japanese Patent Application Laid-Open No. 6-165669, by providing a culture area for directly irradiating sunlight and a culture area for irradiating transmitted light in the depth direction of the culture tank, light considering the depth direction is provided. There is an internal irradiation type photosynthetic culture device that is used efficiently.
[0005]
[Problems to be solved by the invention]
In the conventional external irradiation type photosynthetic culture apparatus, it is necessary to enlarge a tank wall or the like made of a light-transmitting material such as glass in order to increase the irradiation area. There is a problem that it is extremely difficult to increase the size of the culture tank. In addition, in this external irradiation type photosynthetic culture device, when the concentration of the photosynthetic microorganisms is high, the distance that the light reaches is short, and there is a problem that the amount of supplied light is insufficient and the growth of the photosynthetic microorganisms is suppressed. I have.
[0006]
On the other hand, in the internal irradiation type photosynthetic culture apparatus, it is possible to increase the light emission area by increasing the number of light scatterers attached to the inside of the tank. Avoid distance problems.
[0007]
However, the internal irradiation type photosynthetic culture device basically needs to condense sunlight or artificial light and transmit it to the inside of the culture tank. When using sunlight in an internal irradiation type photosynthetic culture device, as a device for condensing, there are a condensing lens and a condensing mirror. Only direct light incident as parallel rays can be used. There is a problem on the efficiency of use of sunlight that scattered light generated by the reflection of the atmosphere or clouds cannot be incident and used.
[0008]
Further, in order to condense the light to be supplied to the internal irradiation type photosynthetic culture device, the light receiving surface of the condenser needs to keep a certain angle (for example, vertical) with respect to the direct light, and for that purpose The trouble is that you have to track the sun. Furthermore, it is difficult at present to condense and transmit 100% of sunlight (direct light) with these condensing devices. Also, when using artificial light, basically, like sunlight, it is necessary to collect light, transmit it into the culture tank, and radiate it. Media is required.
[0009]
As described above, the internal irradiation type photosynthetic culture device having the conventional light-collecting function has a problem in that the light use efficiency is low, and a large cost is required for a light use system including light collection, transmission, and radiation.
[0010]
In view of the above-mentioned problems, the present invention provides a photosynthetic culture device having a high light utilization efficiency and a high photosynthetic ability per unit light receiving area, that is, a CO 2 fixing ability, with a simple device, to prevent global warming. The purpose is to contribute.
[0011]
[Means for Solving the Problems]
In order to solve the above problems, the present invention provides a photosynthetic culture device for culturing photosynthetic microorganisms, wherein a culture tank, a culture solution contained in the culture tank, and light are supplied to the inside of the culture solution. has not provided a condenser lens which is immersed in the culture liquid for planar optical scatterer, the top of the optical synthetic culture device forms a light receiving portion of the solar light, the light receiving unit, sunlight A photosynthetic culturing apparatus, comprising: a culture solution surface to which direct light and scattered light can be incident; and a light incident end face of the light scatterer to which direct light and scattered light of sunlight can be incident.
[0012]
In the photosynthetic culture device, the light scatterer may be a flat light scatterer having a function of scattering and emitting incident light from a surface other than the light incident end face. In the photosynthetic culture apparatus, the light scatterers are vertically arranged such that the end opposite to the light incident end face is located at the bottom of the culture tank, and are arranged in a plurality of rows at intervals on the same plane. It is characterized by having.
[0013]
In the photosynthetic culture apparatus, a gas containing carbon dioxide can be discharged as bubbles from the bottom of the culture tank, and the discharged bubbles can rise between a plurality of rows of light scatterers and on the surface of the light scatterers, The rising of the air bubbles causes a vertical circulating flow of the culture solution, and the circulating flow has a function of constantly updating the culture solution near the light receiving portion on the surface of the culture solution. And in the vicinity of the light scatterer.
[0014]
The light scatterer used in the photosynthetic culture device of the present invention has a structure in which the light incident end face is not provided with a condenser lens or the like, so that both direct light and scattered light can be incident. In addition, the light receiving section is composed of a culture solution surface and light incident end faces of a plurality of rows of light scatterers that maintain a certain interval and protrude from the culture solution surface, and any surface of the light receiving portion is directly reached. Since both light and scattered light can be incident, both sunlight and artificial light can be efficiently incident and used.
[0015]
Since the light scatterer used in the photosynthetic culture apparatus of the present invention can scatter and radiate the incident light on all surfaces except the light incident end face, the light scatterer converts the incident sunlight or artificial light. It can be efficiently supplied into the culture solution in the culture tank. In the photosynthetic culture apparatus of the present invention, since the light scatterer is immersed in the deep part of the tank where light cannot be supplied from the liquid surface, light can be supplied to any part of the culture tank, and the direct light such as a conventional open pond can be used. The photosynthetic ability per unit light receiving area is higher than that of the photosynthetic culture device of the light receiving type.
[0016]
In the photosynthetic culture apparatus of the present invention, gas containing CO 2 is discharged as bubbles from the bottom of the culture tank, and the agitation and mixing can be performed so as to generate a vertical flow of the culture solution by a lift effect due to the rise of the bubbles. Therefore, it is possible to positively promote the renewal of the culture solution in the vicinity of the upper liquid surface of the culture tank where the light supply amount is the largest, and therefore, promote the cultivation of photosynthetic microorganisms, and photosynthetic ability per unit light receiving area, 2 Improve fixability.
[0017]
Further, the plate surface of the plate-shaped light scatterer becomes a space partitioned by other opposing plate surfaces, and a vertical circulation flow portion of the culture solution is formed in this space, so that the vertical circulation flow of the culture solution is further increased. The above-mentioned renewal effect of the culture solution can be enhanced by devising the arrangement of the light scatterers so as to be smooth.
[0018]
The photosynthetic culturing apparatus of the present invention has a plurality of rows of independent light scatterers kept at a constant interval in the culture tank, and can supply light at a uniform light supply density into the culture solution. Even when the scale-up is performed, the light supply density can be easily made constant, and the photosynthetic culture apparatus can be increased in size.
[0019]
BEST MODE FOR CARRYING OUT THE INVENTION
An embodiment of the present invention will be described with reference to the drawings. FIG. 1 is a diagram showing an example of the overall outline of a photosynthetic culture device according to the present invention. FIG. 2 is a sectional view taken along the line A-A in FIG. In the figure, reference numeral 1 denotes a culture tank, and 2 denotes a flat light scatterer immersed in a culture solution 8 contained in the culture tank 1. The flat light scatterer 2 has a light incident end face 3 at one end thereof, and can radiate light entering from the light incident end face 3 from the entire surface of the flat light scatterer 2 other than the light incident end face 3. It is a structure.
[0020]
As the flat light scatterer 2 in the photosynthetic culture device of the present invention, for example, a light scatterer described in JP-A-8-262232 can be used. The light scatterer described in the publication has a structure in which a light scattering surface is formed and light incident from an end face can be uniformly scattered from the entire transparent plate.
[0021]
The flat light scatterer 2 in the photosynthetic culture apparatus of the present invention has one end face as it is as a light incident end face, and has a structure that does not have a condensing function, so that both direct light and scattered light are simultaneously incident. Has the ability to do. The plate-shaped light scatterer 2 has a vertically long shape, and a plurality of rows are vertically arranged in the culture tank 1 at regular intervals on the same plane so that the vertical direction is the depth direction of the culture tank 1. It is arranged so that the end opposite to the light incident end face 3 is located at the bottom in the culture tank.
[0022]
However, the shape of the flat light scatterer 2 in the photosynthetic culture device of the present invention is not limited to a vertically long shape. In addition, the structure is not limited to the light scatterer described in Japanese Patent Application Laid-Open No. 8-262232, as long as the structure allows light to enter from one end surface and scatter and radiate the incident light from the entire surface.
[0023]
Reference numeral 4 denotes a culture solution surface, and the light incident end face 3 of the flat light scatterer 2 is disposed so as to protrude above the culture solution surface 4 in order to directly receive light. 5 is sunlight or artificial light. Reference numeral 6 denotes a ventilation pipe for supplying CO 2 required for photosynthesis, and the ventilation pipe 6 communicates with a sparger 10 for discharging a gas containing CO 2 . The sparger 10 has many fine openings suitable for discharging gas, and is disposed at the bottom in the culture tank 1 and in the vicinity of each flat light scatterer 2.
[0024]
Sunlight or artificial light 5 is supplied to the culture solution surface 4 and the light incident end surface 3 of the flat light scatterer 2. The light to be supplied can be either direct light or scattered light. The light entering from the light incident end face 3 of the flat light scatterer 2 is scattered and radiated from the entire surface of the flat light scatterer 2 and supplied to the deep part of the culture tank 1.
[0025]
The air 7 containing CO 2 is discharged from the bottom in the culture tank 1 upward through the sparger 10 shown in FIG. Air 7 including the exhaled CO 2 supplies the CO 2 to photosynthetic microorganisms, causing the vertical circulating flow of broth as culture medium flow pattern 11 shown by an arrow in FIG. 2 by gas lift effect. This circulating flow promotes the renewal of the culture solution 8 near the culture solution surface 4 with a large amount of supplied light, and enables photosynthetic microorganisms to perform efficient photosynthesis. The upper part of the photosynthetic culture apparatus may be open, and if necessary, for example, a light-transmissive lid may be provided for the purpose of gas management, prevention of contamination, and the like.
[0026]
As shown in FIGS. 1 and 2, the flat light scatterers 2 are arranged in a plurality of rows on the same plane so that a wall having a gap is formed on the flat surface thereof. By discharging the gas 7 containing CO 2 from the bottom of the culture tank 1 between the two walls, the culture solution 8 is smoothly and two-dimensionally moved toward the wall formed by the flat light scatterers 2. The vertical up-and-down circulation flow occurs.
[0027]
Reference numeral 8 in FIG. 1 denotes a culture solution to be supplied, which supplies a micronutrient source or the like to the photosynthetic microorganisms in the culture tank 1. The 9 is withdrawn culture, can be effectively utilized, such as the separated collected photosynthetic microorganism is a CO 2 fixation product from the withdrawn culture 9.
[0028]
【The invention's effect】
According to the photosynthetic culturing apparatus of the present invention, sunlight or artificial light can be directly supplied to the culture solution from the culture solution surface, and light can be supplied from the light incident end of the light scatterer to the deep portion of the culture tank. High photosynthetic capacity per area.
[0029]
In addition, according to the photosynthetic culture device of the present invention, since both direct light and scattered light can be used, the efficiency of using sunlight is high. In particular, since the light incident end face of the light scatterer for internal irradiation can also enter the direct light and the scattered light, the sunlight use efficiency is higher than that of the conventional internal irradiation device that receives only direct light.
[0030]
Further, according to the photosynthetic culturing apparatus of the present invention, since it has a function of circulating and flowing the culture solution such that the culture solution having a large amount of received light near the surface of the culture solution that is directly irradiated with sunlight or artificial light can be renewed, Photosynthesis can be performed efficiently.
[0031]
In addition, according to the photosynthetic culture device of the present invention, since the light scatterer is not provided with a condensing device, a light transmission fiber, and the like, the construction cost is significantly reduced as compared with the conventional condensing type photosynthetic culture device. Has an effect that can be.
[0032]
By using the photosynthetic culture device according to the present invention, CO 2 , which is one of the substances causing global warming, can be biologically fixed efficiently, and can contribute to global environmental conservation.
[Brief description of the drawings]
FIG. 1 shows an overall outline of a photosynthetic culture apparatus of the present invention.
FIG. 2 shows an A-A cross section of FIG.
[Explanation of symbols]
Culture 10 spargers 11 culture flow pattern extracting air 8 cultures 9 containing 1 fermenter 2 planar optical scatterers 3 light incident end face 4 culture surface 5 sunlight or artificial light 6 vent pipe 7 CO 2

Claims (2)

光合成微生物を培養するための光合成培養装置において、
培養槽、該培養槽内に収容されている培養液、及び光を該培養液内部に供給するための該培養液に浸漬されている集光レンズを具備しない平板状光散乱体を有し、
該光合成培養装置の上部は、太陽光の受光部を形成し、
該受光部は、太陽光の直達光及び散乱光が入射可能な培養液面と、太陽光の直達光及び散乱光が入射可能な該光散乱体の光入射端面より構成されることを特徴とする光合成培養装置。
In a photosynthetic culture device for culturing photosynthetic microorganisms,
A culture tank, a culture solution contained in the culture tank, and a flat light scatterer without a condenser lens immersed in the culture solution for supplying light into the culture solution,
The upper part of the photosynthetic culture device forms a light receiving part for sunlight ,
Light receiving unit includes a feature the direct light and the scattered light and the culture liquid surface possible incidence of sunlight, that the direct light and the scattered light of the sunlight is composed of the light incident end face of the possible incident light scatterer Photosynthetic culture device.
前記集光レンズを具備しない平板状光散乱体は、太陽光の光入射端面に対して反対側の端部が培養槽底部に位置するように垂直に配置され、且つ同一平面上に間隔を空けて複数列配置されており、且つ二酸化炭素を含むガスを培養槽底部から気泡として吐出可能で、吐出される気泡が複数列の光散乱体間及び該光散乱体の表面上で上昇することができ、該気泡の上昇により培養液の上下方向の循環流動を生じさせ、該循環流動により培養液面の受光部付近の培養液が常時更新されることが可能な機能を有する通気配管を培養槽底部で且つ該光散乱体の近傍に設けることを特徴とする請求項1記載の光合成培養装置。The flat light scatterer without the condensing lens is vertically arranged such that the end opposite to the light incident end face of sunlight is located at the bottom of the culture tank, and is spaced on the same plane. Are arranged in a plurality of rows, and a gas containing carbon dioxide can be discharged from the bottom of the culture tank as bubbles, and the discharged bubbles can rise between the plurality of rows of light scatterers and on the surface of the light scatterers. The rising of the bubbles causes a vertical circulating flow of the culture solution, and the ventilation flow has a function of constantly updating the culture solution near the light receiving portion on the culture solution surface by the circulating flow. 2. The photosynthetic culture device according to claim 1, wherein the photosynthetic culture device is provided at the bottom and near the light scatterer.
JP01452197A 1997-01-10 1997-01-10 Photosynthetic culture device Expired - Fee Related JP3565468B2 (en)

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