JP3504612B2 - Dietary supplement - Google Patents

Description

Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a dietary supplement, and more particularly to a dietary supplement comprising a combination of cabbage, agaricus and licorice and having a synergistic anticancer effect. [0002] Health foods have become a boom, and various health foods have been developed to improve lifestyle-related diseases, but most of them have been molded by adding excipients to a single material. Things. In recent years, a combination of a plurality of food materials has been developed to promote synergy, and has been marketed. For example, it is said that a health food containing bifidobacteria, dietary fiber and oligosaccharide synergistically exerts a bowel-regulating effect and has a colorectal cancer-preventive effect that cannot be achieved by itself. [0003] However, when a plurality of food materials are combined in this manner, the effects of each other may be offset in reverse. Therefore, when combining food ingredients, for each combination, determine whether the effects are offset and whether a synergistic effect is obtained.
Confirmation by experiment is required. On the other hand, cancer, which is a modern adult disease, still has a large number of patients, and research on cancer treatment and prevention is being actively conducted. In particular, even among the general public, there is a high awareness of cancer prevention, and there is a demand for the development of nutritional supplements that are inexpensive and have high anticancer activity. [0004] In view of the above circumstances, an object of the present invention is to combine a plurality of food materials having an anti-cancer effect so that the anti-cancer effects of each food material influence each other. Accordingly, it is an object of the present invention to provide a dietary supplement having a synergistic anticancer effect which cannot be obtained by using these materials alone. Means for Solving the Problems As a result of intensive studies, the present inventors have found that a food material prepared using cabbage, a food material prepared using mycelium of Agaricus, and licorice are used. The present inventors have found that a dietary supplement excellent in anticancer activity can be obtained by combining food materials prepared using the present invention, and have completed the present invention. Further, the present inventor has further studied the functionality of the dietary supplement and found that the dietary supplement also exerts an anti-stress effect, thereby completing the present invention. [0006] The object of the present invention is to provide a food supplement prepared using cabbage, a food material prepared using mycelium of agaricus , and licorice. It is solved by including a food material prepared by utilizing. As described above, a food material prepared using cabbage containing isothiocyanates having a carcinogenesis preventing action and a mycelium of Agaricus containing β-glucans having an immunostimulating action are prepared. Food material and a food material prepared using licorice containing chalcone with a cancer cell growth inhibitory effect, by inactivating carcinogenic substances each of these food materials has It is possible to obtain a dietary supplement having a carcinogenic preventive action, an immunostimulatory action, and a cancer cell growth inhibitory action. Further, in the present invention, a food material prepared by using cabbage containing isothiocyanates, and a mycelium of Agaricus containing β-glucans
And the food material prepared using licorice containing chalcones, the anticancer effects of these food materials are mutually amplified. Therefore, it is possible to obtain a dietary supplement having a synergistic anticancer effect that cannot be obtained by using these food materials alone. Furthermore, the dietary supplement of claim 1 has an anti-stress effect. [0007] Hereinafter, a dietary supplement according to an embodiment of the present invention will be described. The configuration described below does not limit the present invention, and can be variously modified within the scope of the present invention. The dietary supplement according to the present embodiment is a food material prepared using cabbage containing isothiocyanates, β-
A food material prepared using agaricus mycelium containing glucans and a food material prepared using licorice containing chalcones are blended. [0008] In this embodiment, the food processed cabbage
Use materials. Ground cabbage tissue, or
Cabbage seeds are used by adding water and grinding.
Grinding cabbage tissue and seeds in this way
Miroshi, an endogenous enzyme contained in tissues and seeds
The enzyme acts on glucosinolate to produce isothiocyanate.
Foods containing isothiocyanates for conversion to
It is possible to obtain the material. Incidentally, food Material containing isothiocyanates, for example, processed cabbage, cauliflower, broccoli, kale, cabbage, radish (radish), radish sprouts, radish, Chinese cabbage, turnip, mustard spinach, mustard, a cruciferous plant such as Brussels sprouts It is also possible to use food materials. Specifically, those that have been ground tissue of the Brassicaceae, or Rukoto used <br/> is possible what was triturated by the addition of seeds to the water of the Brassicaceae. [0009] For example, when the tissue, seeds and the like of the cruciferous plant are finely ground or ground, glucosinolate actively reacts in the presence of myrosinase catalyst. Can be increased in isothiocyanate content. After the step of grinding and processing the cruciferous plant tissue and seeds, vitamin C (ascorbic acid) or a salt thereof is added to 0.1%.
Vitamin C added to a concentration of 001 to 1 mM
Either by performing the addition step, or by performing the step of allowing the above-mentioned cruciferous plant tissue, the ground material of the seed to react at 20 to 50 ° C. for a predetermined period of time, to process the cruciferous plant in the food material It is also possible to increase the isothiocyanate content. Conversely, if the concentration of ascorbic acid in the above-mentioned cruciferous plant tissues and ground seeds is higher than 1 mM, the activity of myrosinase is inhibited,
Vitamin C in the above-mentioned cruciferous plant tissues and ground seeds
Adjust so that the (ascorbic acid) content does not exceed 1 mM. [0010] isothiocyanates (R-N = C = S )
Is a component generated by the cleavage of the saccharide portion of glucosinolate by the enzyme myrosinase.
Phenylethyl-isothiocyanate, various sulforaphane (1-isothiocyanato- (4R)-(methylsulfinyl) buta
ne), benzyl isothiocyanate and the like.
Isothiocyanates are known to have anticancer activity. It is thought that it induces the expression of quinone reductase (QR) and glutathione S-transtransferase (GST), which are enzymes involved in inactivating carcinogens, to prevent cancer. In addition, the cruciferous plant is a high nutritional value plant containing vitamins such as vitamins U and C, minerals, and dietary fiber in addition to containing isothiocyanate. Are suitable. The food material prepared using cabbage is blended with other food materials in the form of a squeezed powder of a ground material of the above-mentioned cruciferous plant or the like. Further, it may be blended with other food materials in the form of a crushed product, a crushed product, a crushed product, a squeezed product, a dried product, a powder, an extract, a concentrate, or the like. In addition, as a food material prepared using cabbage, a cabbage fermented extract powder as described in JP-A-2000-245391 may be used. The cabbage fermented extract powder is obtained by pulverizing the washed cabbage with a cutter mixer, under predetermined conditions, for example, 3 kg of cabbage, 1 L of water,
Dried lactic acid bacteria (Lactobacillus plantum) powder 0.5g
Is added, and the mixture is stirred for a predetermined temperature and time (for example, 3
After fermentation at 0 ° C for 2 days, the resulting cabbage fermented product is centrifuged to collect the supernatant, which is heated, concentrated, heat-sterilized, and spray-dried with 200 g of dextrin per 3 kg of cabbage. I do. Instead of the cabbage fermented extract powder, a cabbage fermented extract or a cabbage fermented extract paste obtained by heating, concentrating, heat sterilizing, or adding dextrin to the supernatant of the above cabbage fermented product may be used. In the present embodiment, generally referred to as agaricus
Using agaricus mushrooms belonging to the genus Agaricus
The Agaricus is suitable because of its high β-glucan content.
is there. Agaricus contains not only β- (1,3) glucan but also β- (1,6) glucan, and these polysaccharides act in combination to activate lymphocytes such as macrophages, T cells, and NK cells. It exerts an immunostimulatory effect.
Incidentally, food-containing material containing β- glucans, e.g., himematsutake belonging to agaricaceae (Agaricus blazei), Agaricus (Agaricus campestris), shiitake belonging to Kinshimeji family (Lentinus edodes), reishi belonging to Polyporaceae (Ganoderma Lucidum) , Choreimaitake (Po
lyprous umbellatus, agaric mushroom (Coriolus versicol)
or), Matsuhod (Poria cocos), Maitake (Glifola)
frondosa) Tricholoma matsutak
e), oyster mushroom (Pleurotus ostreatus), enokitake (Flammulina velutipes), hon-shimeji (Lyophyllum a)
ggregatum), which belongs to the family Amanitaceae
It is also possible to use basidiomycetes such as parassis crispa)
There is . Laminaran (laminara), a type of β-glucan
n; laminarin), a food material prepared using a brown algae of the family Laminariaceae, a baker's yeast having a linear glucan having β-1,3 bonds and β-1,6 bonds, or the like may be used. Fungi and algae may be natural products or cultures. [0013] Further , a purified polysaccharide in which glucose has a β-1,3 bond or β-1,6 bond may be used. Further, β-1,3-branched β-1,6 glucan, β-
A 1,6-branched β-1,3 glucan, or a heteroglycan or protein complex mainly composed of these glucans may be used. For example, Pori, a purified product of lentinan isolated from the fruit body of shiitake mushroom, Pori
a cocos) isolated pachyman
And purified products obtained by removing side chains from these purified products to enhance antitumor properties . Food materials containing mycelium of Agaricus,
It is blended with other food materials in the form of mycelium powder of a raw material such as agaricus. Moreover, you may mix | blend with other food materials in the form of a mycelium extract, such as agaricus, a fruiting body extract, and a fruiting body powder. Moreover, you may mix | blend in the form of the refined product of (beta) -glucans. In addition, among basidiomycetes, in addition to β-glucan, those containing vitamins, and those exhibiting blood pressure lowering effect, blood sugar suppressing effect, anticholesterolemia improving effect, antiallergic effect, etc. Basidiomycetes are also suitable as a food material to be added to dietary supplements because they have other functions. In the present embodiment, the licorice is used for adjustment.
Food materials used. Chalcone (chalcone) Foods containing, including the material, for example, leguminous of licorice, including Iisoliquiritigenin, 4-Hydroxyderricin of Umbelliferae, including Ashitaba, of the lily family shallot, Asteraceae of safflower, from Thailand ginger family plant Kurachai (finger Routes), cars (galanga), propolis and the like can also be used. Also, Iisoli, a glycoside of Iisoliquiritigenin contained in licorice
quiritin, Licochalcone A, Licochalcone B, Echinati
Chalcones such as n, Echinadion, Xanthoangenol, and Ashitabchalcone may be used. Licorice is a medicinal herb that has been used since ancient times in the prescription of herbal medicines, Siberia, Mongolia,
There are many varieties, including Ural licorice from Northeast China and those from Japan, a lily family wild plant. Glycyrrhizin contained in licorice has anti-cancer, anti-gastric ulcer action,
It is widely known that it has an anti-inflammatory effect and a liver function improving effect. The chalcone is malonyl-acetyl-Co.
It is a C6-C3-C6 type compound formed by combining A with p-cromile or caffeoyl CoA derived from shikimic acid. Chalcone is considered a biosynthetic precursor of flavonoids, isoflavonoids. Chalcones are known to have antibacterial, antifungal, and cancer cell growth inhibitory effects. The growth inhibitory action of cancer cells is obtained by chalcones inhibiting cyclooxygenase specifically expressed in cancer cells. Sweet
Food materials prepared using grass and containing chalcones are blended with other food materials in the form of an extract powder of a raw material such as licorice. Moreover, you may mix | blend with other food materials in the form of the pulverized material, powder, extract, concentrate, etc. of these raw materials. Also,
You may mix | blend in the form of the purified product of chalcone. The dietary supplement according to the present embodiment comprises a food material prepared using the cabbage and an agaricus.
And a food material prepared by using licorice at the following ratio. A food material prepared using cabbage is blended in a dietary supplement such that the solid content is 10 to 80% by weight, preferably 20 to 50% by weight. Adults can use isothiocyanate and glucosinolates which can be converted to isothiocyanate daily, for a total of 1-10
The amount that can be taken is 00 μmol. The food material prepared using Agaricus mycelium contains β-glucan in a dietary supplement,
It is blended so as to contain 0.5% by weight or more. When a dry powder of Agaricus mycelium having a β-glucan content of 25% is used, the dry powder is blended in a dietary supplement at 2% by weight or more, preferably 5 to 70% by weight. Adults are given β-glucan in an amount of 50 to 1000 mg per day as a guide. The amount may be appropriately changed depending on the condition of the target layer of the dietary supplement. The food material prepared using licorice is blended in a dietary supplement so that chalcones are contained in an amount of 0.1% by weight or more. When licorice extract powder is used as a food material prepared using licorice, this licorice extract powder is incorporated in a dietary supplement at 1 to 50% by weight. The amount of chalcones that can be taken by an adult per day is 50 to 500 mg. The daily intake of each of the above-mentioned food materials is a guide and may be appropriately changed depending on the purpose. Alternatively, the intake may be divided into two or three times a day, so that the total intake per day may be ingested. In addition, the amount of each of the food ingredients is not limited to the above-mentioned amount, but may be a condition of a target layer of a dietary supplement,
It can be changed as appropriate depending on the age group, gender, and the like. Also,
In this embodiment, a food material prepared using cabbage, a food material prepared using agaricus mycelium, and a food material prepared using licorice are blended. are doing. The dietary supplement according to the present embodiment comprises a food material prepared using the cabbage and an agaricus.
A food material prepared using mycelium and a food material prepared using licorice are blended, excipients and the like are added, and the mixture is shaped into a tablet or pill. .
If necessary, capsules such as hard capsules and soft capsules, powders, granules, tea, tea bags, candy, liquids, pastes and the like may be used. And further, for example, royal jelly,
Nutrients such as vitamins, proteins, calcium such as eggshell calcium, chitosan, lecithin, chlorella powder, ashitaba powder, and moroheiya powder can also be added. Stevia powder, matcha powder, lemon powder, honey,
The taste may be adjusted by adding reduced maltose, lactose, sugar solution, seasonings and the like. And the dietary supplement according to the present embodiment may be eaten as it is, depending on its shape or taste,
Alternatively, it may be dissolved in water, hot water, milk, or the like, or may be drinkable after leaching the components. The dietary supplement of the present embodiment, it contains a isothiocyanates having anticancer activity, and β- glucans having immunopotentiating activity, and chalcones having antiproliferative activity of cancer cells Have a synergistic anti-cancer effect. Thus, having a synergistic anti-cancer effect is due to different pathways derived from each component, such as a carcinogenic preventive effect by inactivating a carcinogen, an immunostimulatory effect, and a cancer cell growth inhibitory effect. It is considered that a synergistic and comprehensive anti-cancer effect works. The present invention will be described below with reference to examples.
It goes without saying that the present invention is not limited by this embodiment. (Example 1) As described below, Test Example 1 which is an example of the present invention
And foods according to Comparative Examples 1 to 3, which are conventional examples, were prepared, and an experiment was performed to compare the anticancer effects of the foods according to Test Example 1 and Comparative Examples 1 to 3. 1. Preparation of Test Example 1 First, as a food material prepared using cabbage ,
Calibration <br/> betulinic juice powder isothiocyanates are including A Vrana family plant was prepared. After 500 g of raw cabbage was roughly pulverized with a cutter and a slicer, the cabbage was finely pulverized while being ground using a grinding-type fine pulverizer (trade name: Mascolloider, manufactured by Masuko Sangyo) to form a slurry. In addition,
At the time of this grinding, a sodium ascorbate solution was added so that the concentration in the ground material obtained after the grinding was 0.05 mM. Next, the obtained cabbage ground product was pressed to obtain cabbage juice. Thereafter, the cabbage juice was freeze-dried to prepare cabbage juice powder. Then, the prepared cabbage juice powder
0 g and 1.0 g of Agaricus mycelium powder (amount of β-glucan: 25%), which is a basidiomycete containing β-glucan as a food material prepared using the mycelium of Agaricus
Licorice extract powder as a food material prepared using licorice containing chalcone (based on Japanese Pharmacopoeia) 1.0
g was blended to prepare a dietary supplement according to Test Example 1. 2. Preparation of Comparative Example 1 3.0 g of cabbage juice powder prepared by the same procedure as in the preparation of Test Example 1 was used as a food according to Comparative Example 1. 3. Preparation of Comparative Example 2 3.0 g of Agaricus mycelia powder (β-glucan content: 25%) which is a basidiomycete containing β-glucan as a food material prepared by using the mycelium of Agaricus was compared with Comparative Example 2. The food according to item 2. 4. Licorice extract powder (based on Japanese Pharmacopoeia) 3.0 g as a food material prepared using licorice containing chalcones prepared in Comparative Example 3
Was defined as a food according to Comparative Example 3. The dietary supplement according to Test Example 1 and the foods according to Comparative Examples 1 to 3 thus prepared were administered to rats, and the dietary supplement of Test Example 1, which is an example of the present invention, was used as an anticancer drug. An experiment to confirm the effect was performed. 7 each consisting of 4 animals
Each week-old rat group was gavage-administered once a day for a total of 25 weeks, with 50 mg / kg · body weight of a feed consisting of Test Example 1 and Comparative Examples 1-3. At this time, from the 3rd week after administration of the feed, NMBA (N-nitros) which is a carcinogen was administered while administering the feed consisting of Test Example 1 and Comparative Examples 1 to 3.
obenzylmethylamine) 0.5mg / kg
Administration for 15 consecutive weeks. For the next eight weeks, the first two
As with the weeks, only each feed was given. The control group of rats was fed only a common diet containing neither cabbage juice powder, Agaricus mycelium powder, or licorice extract powder for 25 weeks, and NMBA was administered for 3 weeks as in the other rat groups. The administration was continued for 15 weeks from the week. After a total of 25 weeks of breeding, rats were dissected and observed for cancer incidence and frequency. Table 1 shows the results of this observation. [ Table 1] As shown in Table 1, the control rat group had a cancer prevention rate of 0%, and the rat groups of Comparative Examples 1 to 3 had a cancer prevention rate of 25 to 50%. In one group of rats, the cancer inhibition rate was 100%. In the control rat group, the occurrence of cancer was 14.5 ± 4.
2. In the rat groups of Comparative Examples 1 to 3, the number of cancer occurrences was 3 to 4, whereas in the rat group of Test Example 1, the number of cancer occurrences was 0. From the above results, it was found that the dietary supplement of Test Example 1, which is one example of the present invention, had an excellent anticancer effect. That is, the food material prepared by utilizing the key Yabetsu containing isothiocyanate, beta-
By combining the food material prepared using the mycelium of Agaricus containing glucan and the food material prepared using licorice containing chalcone, as in Comparative Examples 1-3, each alone It was found that the compound showed a synergistic anticancer effect that could not be obtained when administered. Further, a material containing β-glucan such as Agaricus is very expensive, but in Test Example 1, an isothiocyanate was added to an appropriate amount of β-glucan-containing material without using a large amount of expensive β-glucan-containing material. Inexpensive dietary supplements with excellent anti-cancer effects can be produced by combining glycerides and chalcones, so that the dietary supplement of Test Example 1, which is one embodiment of the present invention, is also excellent in cost. Things. [0028] Example 2 In this example, the dietary supplement according to Test Example 1 which is an embodiment of the present invention, the food was prepared according to the comparison example 1-3 is a conventional example, Test Example 1, pair An experiment was performed to compare the anti-stress effects of the foods in proportions 1 to 3. First, a dietary supplement according to Test Example 1 as an example of the present invention and foods according to Comparative Examples 1 to 3 as conventional examples were prepared in the same procedure as in Example 1 above. The prepared dietary supplement according to Test Example 1 and the foods according to Comparative Examples 1 to 3 were administered to rats, and the anti-stress effect of the dietary supplement of Test Example 1, which is an example of the present invention, was confirmed. An experiment was performed. To each group of 6-week-old Wistar (male) rats, 50 mg / kg of body weight consisting of Test Example 1 and Comparative Examples 1 to 3 was administered once a day for 10 times each.
After gavage administration using a gastric tube for one day, the animals were fasted for 24 hours. Then, water immersion restraint stress (23 ± 1 ° C, 7
Hours). The same procedure was performed for the control group of rats, except that only a general feed that did not contain any of cabbage juice powder, Agaricus mycelium powder, and licorice extract powder was given. Then, the stomach of the stressed rat was removed and fixed, and then the length of the ulcer was measured to calculate the total. Table 2 shows the sum of the major diameters of the ulcer. [ Table 2] As shown in Table 2, the total length of ulcers in the control rat group was about 42.5 mm, and the total length of ulcers in the rat groups of Comparative Examples 1 to 3 was about 30 to 43 mm. In the rat group of Test Example 1, the total length of the major axis of the ulcer was extremely small at 6.8 mm. From the above results, it was found that the dietary supplement of Test Example 1, which is one example of the present invention, had an excellent anti-stress effect. When the adrenal gland of each rat was also excised and weighed, the weight of the adrenal gland was proportional to the sum of the major axes of the ulcers. This also indicates that the dietary supplement of Test Example 1, which is one example of the present invention, has an anti-stress effect. As described above, the dietary supplement according to the present invention comprises isothiocyanates having a carcinogen-preventing action by inactivating carcinogenic substances and β having an immunostimulatory action.
- and glucans, because it contains the chalcones having a growth inhibition of cancer cells, carcinogenesis preventive action by inactivation of carcinogens which these substances have, immunopotentiating activity, antiproliferative activity of cancer cells It is possible to obtain a dietary supplement comprising Further, in the present invention, since the combination of the isothiocyanates and β- glucans and chalcones, anticancer effect each of these materials have found with each other to amplify each other, it is obtained with these materials alone It is possible to obtain a dietary supplement having a synergistic anticancer effect that cannot be achieved. Furthermore, the dietary supplement according to the present invention has an anti-stress effect.

────────────────────────────────────────────────── ─── Continuation of the front page (56) References JP-A-50-69267 (JP, A) JP-A-10-70 (JP, A) JP-A-6-3033941 (JP, A) (58) Field (Int.Cl. ⁷ , DB name) A23L 1/29-1/308 A23L 1/212 A61K 35/78

Claims (1)

(57) [Claims] [Claim 1] A food material prepared using cabbage, a food material prepared using agaricus mycelium, and a food prepared using licorice A dietary supplement characterized by containing ingredients.