JP3349729B2 - Tonaka leaf extract-containing skin cosmetics. - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION The present invention relates to a skin cosmetic comprising an extract of Tochu leaves (provided that it is applied to the scalp).
And can be used in, for example, pharmaceuticals, quasi-drugs, cosmetics (various preparations used for humans and other animals) and the like.

[0002]

2. Description of the Related Art Eucommia ulmoides Oliv. Is a dried leaf of Eucommia ulmoides Oliv. Tochu is a plant native to central China and is cultivated in various parts of China, including Sichuan, Guizhou, Yunnan, Shaanxi, Hubei, Guangxi, Jiangxi and Henan. It was introduced to Japan in 1923, and is still cultivated in Nagano Prefecture and elsewhere.

[0003] In general, Tonaka as a medicinal substance uses bark.瀝 瀝 久 久 久 あ り あ り 、 、 あ り あ り あ り あ り あ り 、 あ り 、 あ り あ り 、 、 、 、 、 薬 、 、 薬 、 、 薬 薬 薬 薬 薬 薬 、 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 、 、 薬 、 薬 薬 薬 、 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬 薬. It is used as a fertility agent.

[0004] As the main components of the forest bark, iridoids, lignans, cyclopentanoids, gutta perchas and the like have been reported.

[0005] On the other hand, reports on the use of Tochuha have recently been made, and their publications include (1)
Mainly diuresis, antihypertensive (Japanese and Chinese Pharmaceutical Society journal 3, 89-97, 1986),
(2) Enhancement of liver reserve (Journal of the Japanese Society of Pharmaceutical Sciences 4, p.26-34,
1987), (3) Brain and NG108-15 cell membrane adenylate cyclase activity inhibition (Wakaku Pharmaceutical Society Journal 3, p. 328-, 1987),
(4) angiotensin converting enzyme inhibitor (JP-A-4-12413)
7), (5) Growth inhibition of human colon cancer cells (Korean. J. Bioch
em. Vol. 20, No. 2, p. 49-55, 1988), (6) a preventive and therapeutic agent for coronavirus infection in animals (JP-A-4-128237),
(7) Preventive and therapeutic agent for pseudorabies in animals (JP-A-2-5872)
5), (8) the action of reducing excess fat in the subcutaneous area of pets and around organs (Japanese Patent Laid-Open No. 4-16150), and (9) Meat quality improver for cultured fish (Japanese Patent Laid-open No.
3-30638), (10) Meat improver for poultry raising (JP-A-3-19545)
9), (11) Improving the meat and cortex of livestock (Japanese Patent Laid-Open No. 4-1614)
8) has been reported.

However, these pharmacological effects of Tonaka leaf are oral or intraperitoneal administration, and at present, they have not escaped from the area of alternative use of Tonaka bark, and are useful in the field of cosmetics and the like. There is no. Thus, the present inventors have conducted intensive research by examining and pursuing a new and useful method of use and action unique to Tonaka.

As a result, the present inventors have found that the extract of Tochu leaf has a cell activating action, a lipid peroxide production inhibitory action, and an active oxygen supplement removal action, and it is very effective to apply it to skin cosmetics. The inventors have discovered that the present invention has been completed. Hereinafter, the process leading to the present invention will be described.

[0008] The skin covers the surface of the living body and protects the living body from the adverse environment such as ultraviolet rays, active oxygen, bacteria, dust, etc. In other words, the skin is constantly exposed to these environmental factors. It has been hurt.

[0009] Therefore, researches on cosmetics and the like that protect skin from environmental factors that have a bad influence on the inside and outside of a living body and promote regeneration of damaged skin have been conducted for a long time. Furthermore, in recent years, research aimed at beauty has been actively conducted in the background of the psychology of always wanting to be beautiful in addition to the purpose of health.

[0010] A wide variety of pharmaceuticals, quasi-drugs, and cosmetics are currently on the market for the purpose of removing and eliminating factors that have an adverse effect on the inside and outside of a living body. Among them, a cell activating action, a lipid peroxide production suppressing action, an active oxygen supplement removing action, and the like are known.

For example, the cell activating action is an action that literally activates cells and activates the metabolism of the epidermis. In other words, especially in human skin, unlike lower animals, which form hard shells, scales and mucous membranes, and protect internal organs, epidermal cells themselves are continuously renewed and old cells are By having the function of desquashing, it has a protection function. Therefore, the body is not physically protected by the strong outer skin, but the invasion of foreign substances is dealt with and repaired from the outside by its rapid repairing ability. Thus, once its metabolism stagnates, the organism is at risk,
Cell activation plays an important role for healthy skin and thus for the safety of living organisms.

The action of inhibiting the production of lipid peroxide is an action of preventing lipids (oils) present in a living body from being oxidized. In other words, lipid peroxides formed by oxidation of lipids damage cell membranes in vivo, inactivate various enzymes, and denature biological proteins, and adversely affect biological tissues.
These are considered to lead to diseases such as aging and cancerous changes, and in recent years, suppression of lipid peroxidation has been regarded as important in dermatology.

[0013] Further, the action of supplementing and removing active oxygen refers to active oxygen (Oxyg) generated excessively in living tissues such as skin.
en: radicals): OR (O - 2, H 2 O 2, OH ·, a function of removing and erasing chemiluminescence), active oxygen initially generation of the aforementioned lipid peroxide, stains, such as freckles It is a problem as a cause of abnormal pigmentation, inflammation, wrinkles, etc., and the removal / elimination of active oxygen leads to the prevention of skin disorders.

That is, the present inventors have sought a new use of Tochu leaf and have been conducting intensive research, and in the course of the study, Tochu leaf extract showed the above-mentioned cell activating action, lipid peroxide production inhibitory action, active oxygen supplementation. The present inventors have found that they have a removing effect, and have found that it is effective to use the Tonaka leaf extract in skin cosmetics, and have completed the present invention.

[0015]

SUMMARY OF THE INVENTION The present invention relates to a skin cosmetic containing a Tonaka leaf extract. Hereinafter, the present invention will be described in detail.

[0016]

Means for Solving the Problems The present inventors screened the Tochu leaf extract from various aspects, examined its application method and action, and tried to pursue it. The inventors have found that a lipid peroxide production inhibitory action and an active oxygen supplement removal action exist, and the present invention has been successfully accomplished.

Hereinafter, extraction examples will be disclosed and described in more detail. However, the method described below is an extraction example used in a confirmation test of the operation and the like described below, and the method is not limited thereto.

[0018]

[Extraction example 1]

[Extraction with water] Water (1 kg) is added to Tonaka leaf (50 g) or its crushed product, and the mixture is refluxed and extracted at 90 ° C. for 3 hours. This is filtered, and water is added to the filtrate to make 1 L, which is used as a Tonaka leaf extract.

[0019]

[Extraction example 2]

[Extraction with aqueous ethanol] To Nakano leaf (50 g) or its crushed product, a 40% aqueous ethanol solution (1 kg) is added, and the mixture is refluxed and extracted at 60 ° C for 3 hours. This is filtered, and a 40% aqueous ethanol solution is added to the filtrate to make up to 1 L, and this is used as a Tonaka leaf extract.

[0020]

[Extraction example 3]

[Extraction with water-containing polyhydric alcohol] To Tonaka leaf (50 g) or its pulverized product, a 50% aqueous solution of butylene glycol (1 kg) is added, and the mixture is extracted with stirring at 60 ° C for 3 hours. This was filtered, and a 50% aqueous solution of butylene glycol was added to the filtrate.
1 L, which is used as a leaf extract in the forest.

[0021]

[Test Example 1] Cell activation activity test of Tonaka leaf extract

With respect to the Tochu leaf extract obtained by the method shown in each extraction example, the action of promoting the oxygen consumption of rat liver homogenate was examined by a modified method of Warburg.

(A) Test method The liver of a guinea pig was taken out, washed well with purified water, and then added with the same amount of cold Krebs-Ringer bicarbonate buffer (KRB) as the liver, and homogenized with a glass homogenizer. Stored in ice water until time. TAIYO O ₂ UP-TESTER (manufactured by Taiyo Kagaku Kogyo Co., Ltd.)
1 mL of the sample, 1 mL of KRB, and 0.5 mL of the liver homogenate are placed in the reaction vessel, and a filter paper moistened with 2N NaOH (CO ₂ absorbent) is attached to the lid of the reaction vessel, and the vessel is tightly closed. Next, the holder is put in a thermostat at 37 ° C., and after 5 minutes, the air purge pin is pushed to draw water into the capillary. After an additional 5 minutes, the air purge pin is pushed, water is brought to a predetermined position, and measurement is started. By 90 minutes after the start of measurement, the amount of oxygen consumed is determined from the amount of water that has moved through the capillary. In addition, the oxygen consumption (μm
L) is a, oxygen consumption (μL) for 90 minutes from the start of measurement when purified water is added instead of the sample is b, and oxygen consumption (μL) when neither the sample nor the liver homogenate is added is c, The oxygen consumption promotion rate was determined by Equation 1.

[0024]

(Equation 1)

[0025]

[Table 1]

(B) Test results The results are shown in Table 1, as shown in Table 1.
In 3), stronger promotion of oxygen consumption was obtained than in the case where purified water was set to 100.0%, and a cell activating effect was confirmed.

[0027]

[Test Example 2] Test of lipid-peroxide-suppressing action of Tonaka leaf extract

For the Tonaka leaf extract obtained by the method shown in each extraction example, the peroxide of linolenic acid was determined by the TBA method (Analytical, Biochemistry, Vol. 95, p. 351-358, 1979). The production inhibitory effect was examined.

(A) Test method 0.1% linolenic acid in 0.8% aqueous sodium lauryl sulfate solution
And dissolve, and transfer 3.9 mL of this solution to a 10 mL screw bottle. 0.1 ml of the sample solution is added to this, and ultraviolet light (Toshiba F
Three L-20SE lamps and three FL-20SBLB lamps are irradiated in parallel at a distance of 15 cm. ) For 1 hour, take 1 mL of this solution, and add 1.5 mL of 0.8% thiobarbituric acid aqueous solution and 20%
Add 1.5 mL of acetic acid (pH 3.5) and heat at 95 ° C for 1 hour. After cooling, 1 mL of purified water and n-butanol: pyridine (1
5: 1) Add 5 mL, shake well, and centrifuge. This n
-The absorbance at 532 nm of the butanol layer was measured to determine the amount of lipid peroxide. In addition, the amount of lipid peroxide when the sample is added and irradiated with ultraviolet light is a, the amount of lipid peroxide when the sample is not irradiated with ultraviolet light is b, and when the sample is irradiated with ultraviolet light without adding the sample. The lipid peroxide suppression rate was determined from Equation 2 as the lipid peroxide amount a ′ of Example 2 and the lipid peroxide amount b ′ when no ultraviolet light was irradiated without adding a sample.

[0030]

(Equation 2)

[0031]

[Table 2]

(B) Test results The results are shown in Table 2, as shown in Table 2.
3) was confirmed to have a strong lipid peroxide production inhibitory action.

[0033]

[Test Example 3] Test for removal of active oxygen by Tonaka leaf extract The Tonaka leaf extract obtained by the method shown in each extraction example was subjected to the NBT method of Imana et al.
144, Medical and Dental Medicine Publishing Co., Ltd.), and studied the effect of removing active oxygen.

(A) Experimental method 2.5 mL of 0.05 M Na ₂ CO ₃ buffer (pH 10.2) was added to a small test tube.
75 mM NBT (Nitrofluorotetrasodium), 3.0 mM Xanthine, 3.0 mM EDT
A, 0.15 w / v% BSA (Bobin serum albumih), and 0.1 mL of each sample were added, and the mixture was kept at 25 ° C for 10 minutes. Xanthine oxyterase (the absorbance change of NBT when purified water was added instead of the sample was approximately 0.
(Prepared to be 3/20 minutes) Add 0.1 mL to start the reaction. After 20 minutes, 0.1 mL of 6 mM CuCl ₂ was added to stop the reaction, and the absorbance at 560 nm was measured. The number of active units per 1 mL of the sample was determined by defining the active oxygen removing action for suppressing the absorbance by 50% when purified water was added instead of the sample as 1 unit.

[0035]

[Table 3]

(B) Test results The results are shown in Table 3, as shown in Table 3.
In 3), a strong active oxygen supplement removal effect was obtained.

Hereinafter, the use of the present invention will be described in more detail with reference to a few examples. However, the present invention is not limited to the following examples, and cosmetics in the form of external use on various skins It can be contained and blended in products, drugs, quasi-drugs and the like. In addition, each Example may be what was manufactured by the usual method in the manufacture of each product, and shows only the compounding amount, and the Tochu leaf extract used was one manufactured by a conventionally known method.

The starting material of the present invention is
commiaceae Plant Eucommia (Eucommia ulmoides Oli)
v.) The leaves are dried, but it is also possible to use dried eucommia stem and bark.

Further, the amount of the Tonaka leaf extract of the present invention is 0.001% by weight or more (hereinafter, expressed as% by weight unless otherwise specified), preferably 0.1% by weight or more. The extract is used in various forms such as a solution, a powder, a sol, a gel, an emulsion, and a wax, which are applied to the skin, rubbed, and sprayed (aerosol). In addition, the Tonaka leaf extract is blended with a known skin cosmetic or a base for external preparation of skin by a conventional method, lotion, skin cream, milky lotion,
Drugs such as cosmetics, pharmaceuticals, quasi-drugs (including various preparations used for humans and other animals and plants) for external use on the skin, skin external preparations such as ointments (however,
Excluding those that apply) .
Tochu leaf extract contains vitamins, plant and animal extracts, skin function enhancers, various surfactants, solvents, flavors, coloring agents, fungicides,
It is stable against preservatives, antioxidants, humectants, other various additives, and various cosmetic bases, and can be blended with these and used in combination.

[0040]

Example-1

[Prescription example of peel-off pack]

 Purified water 66.5% Glycerin 5.0 Propylene glycol 4.0 Polyvinyl alcohol 15.0 Ethanol 8.0 Polyoxyethylene glycol 1.0 Methyl parahydroxybenzoate 0.2 Fragrance 0.2 Tonaka leaf extract 0.1 Above Was produced.

[0041]

Example-2

[Prescription example of cold cream] Beeswax
11.0% Liquid paraffin 22.0 Lanolin 10.0 Almond oil 15.0 Borax 0.5 Methyl paraoxybenzoate 0.2 Purified water 40.9 Fragrance 0.3 Tonaka leaf extract 0.1 Cold cream was produced.

[0042]

EFFECT OF THE INVENTION The extract obtained from Tochu leaf of the present invention is
It has a prominent cell activating action, lipid peroxide production inhibitory action, and active oxygen supplement removal action. Therefore, the Tonaka leaf extract of the present invention is applied to skin cosmetics (however, those applied to the scalp).
) Is very effective. In other fields of use, it can be used as an external preparation for pharmaceuticals, quasi-drugs, cosmetics, etc. in the form for external use on the skin. Furthermore, it can be collected and supplied more easily than the traditionally used Chinese traditional medicine (use bark).
It can also be used in place of Tochu (use area: bark).

────────────────────────────────────────────────── ─── Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI A61P 43/00 107 A61P 43/00 107 (58) Fields investigated (Int.Cl. ⁷ , DB name) A61K 7/00-7 / 50 A61K 35/78-35/84 CA (STN)

Claims (1)

(57) [Claims] 1. A skin cosmetic (excluding those applied to the scalp) , which contains an extract of Tochu leaf.