JP3336386B2 - Insect skin incision apparatus and insect skin incision method - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an insect incision apparatus and an insect incision method for incising and / or perforating the outer skin of insects such as silkworms.

[0002]

2. Description of the Related Art In general, in the field of biotechnology, the outer skin of an insect is incised and / or pierced to remove the contents such as body fluids of the insect, or to infect a perforated portion with a recombinant virus such as baculovirus. Measures have been taken. In particular, among insects, silkworms have long been used as production hosts for useful substances, and are subjected to such incision and / or perforation.

As a method for incising silkworms, Japanese Patent Laid-Open No.
Japanese Patent Application Laid-Open No. 90778/90778 discloses a method for incising and perforating the silkworm hull using a high-pressure liquid.
As disclosed in Japanese Patent No. 9238, a method of cutting the epidermis or body of the silkworm by contacting a blade that emits ultrasonic waves, or as described in Japanese Patent Application Laid-Open No. 9-70241, There is known a method of incising the outer skin by contact.

However, it is difficult to cut and / or pierce only the outer skin of the silkworm using any of these techniques, and there is a problem that the silkworm is seriously damaged. In addition, since it is difficult to incise and / or pierce only the outer skin of the silkworm using any of these techniques, the body fluid of the silkworm cannot be taken out in a pure state, and a large amount of There is a problem that impurities are mixed.

[0005] In other words, conventional insect incision techniques such as those disclosed in the above-mentioned publications do not allow accurate incision and / or perforation of insects and cannot extract pure body fluid. Is the current situation.

[0006]

SUMMARY OF THE INVENTION Accordingly, the present invention has been made in view of the above-mentioned problems, and does not unnecessarily damage the insect to be treated, and accurately incises only the outer coat of the insect. It is an object of the present invention to provide an insect incision apparatus and an insect incision method that can perform perforation.

[0007]

An insect incision apparatus according to the present invention, which has achieved the above-mentioned object, comprises a mounting table for fixing and holding an insect to be treated and a mounting table for the insect held on the mounting table. Laser irradiation means for irradiating infrared laser light, wherein the infrared laser light emitted from the laser irradiation means cuts and / or pierces the outer skin of the insect held on the mounting table. is there.

[0008] The insect skin incision apparatus according to the present invention configured as described above irradiates an infrared laser beam to the insect skin, thereby reliably incising and / or piercing the skin to be treated. Since the energy of the infrared laser light is easily absorbed by moisture, it is attenuated by the body fluid inside the outer skin after incising and / or piercing the outer skin. Thus, according to the insect skin incising apparatus, even if the outer skin is incised and / or pierced using infrared laser light, it is possible to maintain the internal tissues and organs covered by the outer skin without damage.

Further, the insect incision apparatus according to the present invention comprises:
A moving means for controlling the movement of the mounting table in a predetermined direction; and a moving means for moving the mounting table moved by the moving means, which is disposed on a stage preceding the laser irradiation means and held by the mounting table. And a position detection means for detecting the position of the insect, which is disposed to face the insect.
It is preferable that the position signal of the insect detected by the position detection means is input to the laser irradiation means, and the insect detected by the laser irradiation means based on the position signal is irradiated with infrared laser light.

[0010] In this case, since the insect incision apparatus includes the position detecting means, the insect laser to be treated can be irradiated with infrared laser light with high accuracy. Therefore,
In this case, according to the insect husk incision device, a desired position of the insect husk can be accurately incised and / or pierced, and
Damage to internal tissues and organs covered by the outer skin can be prevented.

Further, the insect skin incision apparatus according to the present invention preferably has a body fluid extracting means for extracting a body fluid of the insect cut and / or pierced by the laser irradiation means.

Furthermore, an insect incision apparatus according to the present invention is provided in front of the above-mentioned laser irradiating means, for supplying insects to be treated to the mounting table, Preferably, a collecting means is provided for collecting the insects disposed and held on the mounting table.

Further, in the insect incising apparatus according to the present invention, the mounting table has negative pressure generating means for generating a negative pressure for holding the insects, and the negative pressure generated by the negative pressure generating means is provided. It is preferable that the laser irradiation means irradiates the insect with infrared laser light in a state where the insect is suspended vertically downward by holding the insect on the mounting table by pressure.

On the other hand, the insect incision method according to the present invention comprises:
Insects to be treated are held on a mounting table, and the insects held on the mounting table are irradiated with infrared laser light to cut and / or pierce the outer skin of the insects. .

The insect skin incision method according to the present invention configured as described above ensures that the outer skin of the treatment target is incised and / or pierced by irradiating the outer skin of the insect with infrared laser light. In this method, the energy of the infrared laser light is easily absorbed by moisture, so that after the outer skin is incised and / or pierced, the infrared laser light is attenuated by a body fluid or the like inside the outer skin. Thus, according to this insect skin incision method, even if the skin is incised and / or pierced using infrared laser light, the internal tissues and organs covered by the skin can be maintained without damage.

Further, the insect incision method according to the present invention comprises:
It is preferable that the position of the insect held on the mounting table is detected, and the detected insect is irradiated with infrared laser light based on the position information of the insect.

In this case, according to the present method, the insect laser to be treated can be irradiated with infrared laser light with high accuracy. Therefore, in this case, according to the insect incision incision method, a desired position of the insect integument can be accurately incised and / or pierced, and damage to internal tissues and organs covered by the integument can be prevented.

Further, the insect incision method according to the present invention is characterized in that the insect to be treated is held in a vertically suspended state by applying a negative pressure to the insect to be treated, and the insect suspended in the vertically downward direction is held. Irradiation of infrared laser light in the midline direction is preferred.

[0019]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, specific embodiments of an insect incision apparatus and an insect incision method according to the present invention will be described in detail with reference to the drawings. In the following examples, the larva or pupa of the silkworm and the larva of the silkworm that has been frozen or cold anesthetized will be described as examples of the insects to be incised and perforated. In the method, the insects are not limited to silkworms, but may be other insects.

As shown in FIG. 1, the insect incision apparatus to which the present invention is applied includes a plurality of mounting tables 3 fixedly arranged on one main surface of a belt portion 2 of a belt conveyor 1;
The belt portion 2 is opposed to one main surface side of the belt conveyor 1.
A silkworm supply unit 4, a position detection sensor 5, a laser irradiation device 6, and a silkworm collection unit 7, which are sequentially arranged along the moving direction of
The belt portion 2 faces the other main surface side of the belt conveyor 1.
A mounting table cleaning device 8 and a mounting table drying device 9 are sequentially arranged along the moving direction of the mounting table.

The belt conveyor 1 has a pair of driving rollers 10a, 10b at both ends of a belt portion 2 formed in an annular shape.
have. The pair of drive rollers 10a, 10b
, The belt portion 2 can be driven in the direction indicated by the arrow a in FIG. Thus, the mounting table 3 is sequentially transported in the direction indicated by the arrow a in FIG.

The plurality of mounting tables 3 are provided side by side at predetermined intervals, and each has a holding means for holding the silkworm 11. Examples of the holding means for holding the silkworm 11 include an adhesive tape for holding the silkworm 11 horizontally by an adhesive force, and a transparent flat plate or sheet for holding a plurality of the silkworms 11 arranged horizontally. According to these holding means, the movement of the silkworm 11 can be suppressed, and the silkworm 11 can be fixed at a predetermined position on the mounting table 3. Furthermore, by using a frozen silkworm 11 or a low-temperature anesthetized silkworm 11 as the silkworm 11 to be treated, the movement of the silkworm 11 is more reliably suppressed.

The silkworm supply unit 4 holds the silkworm 11 to be treated, and places the silkworm 11 on the mounting table 3. The silkworm supply unit 4 supplies the silkworm 11 to a predetermined position of the mounting table 3 in a horizontal direction, and fixes the silkworm 11 by the holding means of the mounting table 3.

The position detecting sensor 5 is, for example, a CCD for detecting the position of the silkworm 11 held on the mounting table 3.
It has a camera and an optical sensor. When detecting the position of the silkworm 11, the position detection sensor 5 outputs a position information signal to the controller 12, and outputs a movement speed signal of the silkworm 11 corresponding to the movement speed of the belt 2 to the controller 12. Note that the moving speed signal of the silkworm 11 may be input to the controller 12 in advance.

The controller 12 has a position detecting sensor 5
It outputs a control signal to the laser irradiation device 6 based on the position information and the moving speed signal input from the. This control signal is a signal for controlling the irradiation timing and irradiation position of the infrared laser light emitted from the laser irradiation device 6. That is, the controller 12 controls the position at which the silkworm 11 is incised and / or pierced, and controls the irradiation time of the infrared laser light defined at a predetermined output and irradiation speed based on the moving speed of the silkworm 11.

As the laser irradiation device 6, for example, a commercially available laser marker or the like can be used. Further, the laser irradiation device 6 includes a laser displacement device for displacing the position of the emitted infrared laser light and an output control unit for controlling the output of the laser. The laser displacement device and the output control unit are controlled by a control signal from the controller 12. Therefore, the infrared laser light is irradiated to a desired position by the laser displacement device based on the control signal, and the intensity control of the irradiation energy and the irradiation time are turned on / off by the output control unit based on the control signal. Becomes

The operation of the laser irradiation device 6 is controlled by the control signal as described above, so that the silkworm 11 moving at a predetermined speed can be irradiated with infrared laser light for a predetermined time. Thereby, the silkworm 11
Only the outer skin at the predetermined site will be incised and / or perforated.

Further, the insect incision apparatus has a container 13 disposed below the belt section 2 for collecting body fluid spouting from an incision or perforation site. That is,
The bodily fluid spouting from the silkworm 11 cut or pierced by the laser irradiation device 6 is dropped from the opening formed in the belt portion 2, and the dropped bodily fluid can be stored in the container 13.

The silkworm collecting unit 7 is disposed near the last end of the belt unit 2 in the moving direction, and can house the silkworm 11 therein. That is, the mounting table 3 conveyed by the belt conveyor 1 becomes vertical with the movement of the belt unit 2,
After that, a silkworm collecting unit 7 is provided at a position where the silkworm held on the mounting table 3 peels off and falls. In the silkworm collecting section 7, the treated silkworm 11 is collected and the container 1
The remaining body fluid not collected in step 3 can be eluted into the collection solution and collected.

The silkworm collecting section 7 is not limited to the above, and may be, for example, one that sucks and collects the treated silkworms 11 with a suction device, or the mounting table 3.
May be vibrated to separate the treated silkworm 11 from the mounting table 3. In addition, in the silkworm recovery section 7,
The body fluid may be positively collected from the collected silkworm 11 by a suction unit, or the body fluid remaining on the mounting table 3 may be collected by the suction unit. When the body fluid remaining on the mounting table 3 is collected by the suction unit, the collection rate of the body fluid to be collected can be improved.

On the other hand, the mounting table cleaning device 8 is provided to face the other main surface of the belt conveyor 1 and has a nozzle for spraying a cleaning liquid into the mounting table 3 at a predetermined pressure. The mounting table cleaning device 8 includes the silkworm 1 held on the mounting table 3.
The body fluid of the silkworm 11, which has adhered, can be washed out by performing a treatment such as incision on the sample 1.

The mounting table drying device 9 includes the belt conveyor 1
And a dryer for drying the mounting table 3 that has been cleaned by the mounting table cleaning device 8 and that is disposed to face the other main surface of the mounting table. The mounting table drying device 9 can wash the mounting table 3 and then dry the cleaning liquid attached to the mounting table 3 so that the mounting table 3 can be used again.

In the insect skin incision apparatus configured as described above, first, the silkworm 11 to be treated is supplied from the silkworm supply unit 4 to the mounting table 3, and the silkworm 11 is fixed on the mounting table 3. Then, the mounting table 3 is conveyed by the belt conveyor 1 so that the mounting table 3 faces the position detection sensor 5. At this time, the position detection sensor 5
The accurate position of the silkworm 11 fixed to the mounting table 3 is detected and the moving speed of the silkworm 11 is detected, and a position information signal and a moving speed signal of the silkworm 11 to be treated are output to the controller 12.

Next, in the insect incision apparatus, the mounting table 3 is transported by the belt conveyor 1 and the laser irradiation apparatus 6 is used.
To face. At this time, the laser irradiation device 6 receives a control signal output from the controller 12 based on the position information signal and the moving speed signal. Then, the laser irradiation device 6 determines
A predetermined position in the silkworm 11 to be treated is irradiated with infrared laser light having a predetermined intensity at a high speed. Specifically, the irradiation position of the infrared laser light is controlled by the laser displacement device of the laser irradiation device 6, and the output and output timing are controlled by the output control unit of the laser irradiation device 6. However, the output of the infrared laser light may be set in advance.

As described above, by irradiating a desired position of the silkworm 11 with the infrared laser beam, a desired position of the silkworm 11 can be incised or a desired position of the silkworm 11 can be perforated. This infrared racer has a property of burning off the outer skin at the irradiated position and attenuating due to moisture contained in the contents covered by the outer skin. For this reason, the infrared laser light can cut and / or pierce only the outer skin without damaging the contents covered by the outer skin.

Therefore, according to the insect incision apparatus, when the outer skin of the silkworm 11 is incised and the body fluid is collected, the outer fluid, body tissue, midgut fluid and the like are not mixed into the collected body fluid, and only the body fluid is collected. Can be collected with high purity. At the same time, according to this insect incision apparatus, only the outer skin can be incised without lowering the turgor pressure of the silkworm 11, so that the spouting amount of the silkworm 11 can be kept high. Therefore, by using this insect incision apparatus, the useful substance can be obtained with excellent purification efficiency without deteriorating the useful substance in the body fluid.

Further, according to this insect incision apparatus, since infrared laser light is used, when the baculovirus or the like is transmitted to the hole formed by perforating the outer skin of the silkworm 11, the silkworm 11 is not affected. Injury and pain can be significantly reduced. For this reason, it is possible to suppress the weakness of the silkworm 11 that has been perforated and infected with the baculovirus, and it is possible to maintain the useful substance producing ability of the silkworm 11 at a high level.

In particular, in the insect incision apparatus, the incision and / or perforation is performed based on the position information signal and the moving speed signal of the silkworm 11 to be treated. For this reason,
According to this insect skin incision apparatus, a large amount of silkworms 11 can be incised and / or pierced in a short period of time by irradiating infrared laser light at a high speed with an irradiation position device that operates at a high speed.

Also, in this insect incision apparatus, since the silkworm 11 is fixed on the mounting table 3, the silkworm 11 to be treated can be prevented from moving, and the accuracy of the irradiation position of the infrared laser beam can be reduced. It can be further improved. As a result, erroneous irradiation of infrared laser light can be reliably prevented, and the treatment accuracy of the insect incision apparatus can be greatly improved.

Further, in the insect incision apparatus, the treated silkworm 11 is recovered by the silkworm recovery unit 7 and
The mounting table 3 after the silkworms have been collected can be washed and dried, and the mounting table 3 can be reused a plurality of times. That is, by rotating the mounting table 3 along the belt portion 2 of the belt conveyor 1, the incision and / or perforation of the silkworm 11 can be repeatedly performed, and a large amount of the silkworm 11 can be automatically treated. it can.

By the way, the present invention is not limited to the above-described embodiment, and may be, for example, an insect incision apparatus as shown in FIG. That is, as shown in FIG. 2, the insect incision incision apparatus to which the present invention is applied includes a mounting table 20 on which a plurality of silkworms 11 are fixed so as not to overlap with each other, and supplies the silkworms 11 on the mounting table 20. The apparatus includes a silkworm supply and position detecting device 21 for detecting the position of the silkworm 11, and a laser irradiating device 6 provided at a subsequent stage of the silkworm supply and position detecting device 21 to emit infrared laser light.

In the insect incision apparatus, the silkworm supply and position detecting device 21 fixes the plurality of silkworms 11 on the mounting table 20 and determines the positions of the plurality of silkworms 11 fixed on the mounting table 20. Detects planarly. Then, the silkworm supply and position detecting device 21 detects the silkworm 1 in the surface of the mounting table 20.
1 is output to the controller 12.

The controller 12 outputs a control signal for controlling the irradiation position of the infrared laser light to the laser irradiation device 6 based on the position information signal. At this time, the control signal controls the laser irradiation device 6 so that the plurality of silkworms 11 fixed within the surface of the mounting table 20 are sequentially irradiated with the infrared laser light. Therefore, the laser irradiation device 6 sequentially irradiates the plurality of silkworms 11 with infrared laser light based on the control signal output from the controller 12.

As described above, since the insect incision apparatus detects the positions of the plurality of silkworms 11 fixed in the surface of the mounting table 20 at a time, the plurality of silkworms 11 can be treated at a very high speed. Can be applied. In addition, it is particularly preferable that this insect incisor is used when the outer skin of the silkworm 11 is pierced to form a hole.

Further, according to the insect incision apparatus, since the infrared laser beam is used, only the outer skin is incised and / or cut without damaging the internal tissues and organs covered by the outer skin.
Or it can be perforated. For this reason, according to this insect husk incision apparatus, when incising the husk of the silkworm 11 and collecting body fluid, the husk, body tissue, midgut fluid, etc. are not mixed into the collected body fluid, and only the body fluid is highly purified. Can be collected. At the same time, according to this insect incision apparatus, silkworm 11
Since only the outer skin can be incised without lowering the turgor pressure of the silkworm, the spouting amount of the silkworm 11 can be kept high. Therefore, by using this insect incision apparatus, the useful substance can be obtained with excellent purification efficiency without deteriorating the useful substance in the body fluid.

Further, the present invention is not limited to the insect incision apparatus as shown in FIG. 1 or FIG. 2, but is also applicable to the insect incision apparatus as shown in FIG. The insect skin incision apparatus shown in FIG. 3 uses the belt conveyor 1 as the mounting table 3 and arranges a negative pressure generator (not shown) in the belt conveyor 1, and performs treatment by the negative pressure generator. The target silkworm 11 is sucked and held, and a plurality of silkworms 11 to be treated are treated.
Is suspended vertically below the other main surface of the belt portion 2 with the head up and the tail down. In this state, the insect incision device includes the position detection sensor 5 and the controller 1
Incision and / or perforation is performed on the silkworm 11 to be treated by the laser irradiation device 2 and the laser irradiation device 6 as in the above-described embodiment.

In addition, the insect incision apparatus is provided with a container 30 for collecting bodily fluids on the rear side of the laser irradiation apparatus 6.
And a cleaning nozzle sampling device 31. This container 3
No. 0 is disposed at a position facing the silkworm 11 conveyed by the belt conveyor 1 after the incision and / or perforation treatment is performed, and a body fluid extracted by gravity can be collected. Further, the washing nozzle collecting device 31 includes a nozzle unit 32 that sprays a collected liquid for extracting body fluid onto the silkworm 11,
A collection container 33 that collects the body fluid collected by spraying the collected liquid together with the collected liquid. According to the washing nozzle collecting device 31, the bodily fluid that has not been extracted due to gravity can be almost completely collected and collected using the collected liquid.

Further, this insect incision apparatus has a silkworm collecting container 34 disposed at the subsequent stage of the washing nozzle collecting device 31. In this insect skin incision apparatus, the silkworm 11 is dropped into the silkworm collecting container 34 and collected by releasing the suction force applied to the silkworm 11 from which the body fluid has been collected.

In the insect skin incision apparatus configured as described above, by using infrared laser light, it is possible to incise and / or perforate only the outer skin without damaging the internal tissues and organs covered by the outer skin. it can. For this reason, according to this insect incision apparatus, when incising the integument of the silkworm 11 and collecting bodily fluid, the integument or body tissue is contained in the collected bodily fluid.
Only the body fluid can be collected with high purity without the intestinal fluid and the like mixed. At the same time, according to this insect incision apparatus, only the outer skin can be incised without lowering the turgor pressure of the silkworm 11, so that the spouting amount of the silkworm 11 can be kept high. Therefore, by using this insect incision apparatus, the useful substance can be obtained with excellent purification efficiency without deteriorating the useful substance in the body fluid.

In particular, in this insect incisor, the silkworm 11
In a state in which the body fluid is suspended vertically downward, the body fluid is collected almost completely, so that the body fluid is not lost and the collection efficiency of the body fluid is excellent. In addition, since a mounting table cleaning device, a mounting table drying device, and the like are not required for collecting body fluid, the configuration of the entire device can be simplified, and the cost can be reduced.

In each of the above-described examples, the irradiation position of the infrared laser light is adjusted by controlling the laser irradiation device 6 with a control signal. However, the present invention is not limited to such a configuration. That is, the present invention
A driving means for movably driving the mounting table 3 is provided, and a control signal from the controller 12 is input to the driving means to move the mounting table 3 and irradiate a predetermined position with infrared laser light. It may be. That is, in this case, only the output and the irradiation timing of the infrared laser light are controlled. Then, by moving the mounting table 3 itself, a predetermined position in the silkworm 11 to be treated is irradiated with the infrared laser light. Even in this case, the infrared laser light cuts only the outer skin of the silkworm 11 accurately and / or
Or it can be perforated.

[0052]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, an embodiment to which an insect incision apparatus and an insect incision method according to the present invention are applied will be described.
The present invention is not limited to the embodiments described below. [Example 1] In Example 1, an experiment was performed in which the outer skin of the silkworm was incised to collect body fluid. The silkworm used in this experiment was
Male and female silkworms, 5 days old and 4 days old, and 10 males and 5 days old, 5 days old and 5 days old, were used. These silkworms are both "Shin Asagiri".

First, these silkworms were irradiated with an infrared laser beam at an output of 5 W and an irradiation time of 0.5 seconds to perform an incision treatment. At this time, the irradiation relative speed of the infrared laser light was set to 2.5 cm / sec. As a result, an incision could be made with an incision length of 11 mm and an incision width of 0.7 mm.

One minute after the incision treatment, the weight of the body fluid flowing out of the silkworm was measured and examined as the outflow rate. Table 1 shows the results. Here, the outflow rate was calculated as 100 × (body fluid outflow [g]) / (whole silkworm weight before treatment [g]).

[0055]

[Table 1]

As can be seen from Table 1, both the 5th and 4th instar silkworms and the 5th and 5th instar silkworms show high outflow rates. From this, it can be seen that bodily fluids can be efficiently collected by incising silkworms with infrared laser light.

On the other hand, when the treatment is performed using a high-pressure liquid, the tissue of the body of the silkworm penetrates, so that the internal tissues and organs are mixed into the body fluid, and the high-purity body fluid is used. Could not get. In addition, when the treatment is performed using a blade that emits ultrasonic waves, the body fluid comes out in a mist state due to the vibration of the cutting edge caused by the ultrasonic waves (20,000 Hz), so that it is difficult to collect the body fluid. However, it was difficult to incise only the skin, and internal tissues and organs were mixed in the body fluid. Therefore, when a blade that emits ultrasonic waves is used, a complicated apparatus is required to recover silkworm body fluid with high purity. Furthermore, when the treatment was performed with the rotating blade in contact, it was difficult to incise only the outer skin of the silkworm, and internal tissues and organs were mixed into the body fluid. Therefore, when the rotary blade was used, very high-precision control for incising only the outer skin of the silkworm was required.

As described above, when the treatment is performed using the infrared laser beam, it is clear that a highly pure body fluid can be collected with excellent recovery efficiency as compared with any of the above-mentioned methods. Was.

Example 2 In Example 2, an experiment was performed in which bodily fluid was collected by perforating the outer skin of the silkworm. The silkworms used in this experiment were “male shin Asagiri” and “female shin Asagiri” of 5 years old and 5 days old. In Example 2, 11 silkworms were used.

First, these silkworms were subjected to a perforation treatment with infrared laser outputs of 5 W, 7 W, 9 W and 10 W, and irradiation times of 0.1, 0.2 and 0.3 seconds at the respective outputs. gave. The perforation location was one point in the center of the star print.

After the perforation treatment, the weight of the bodily fluid flowing out of the silkworm was measured and examined as the outflow rate. Table 2 shows the results. Here, the outflow rate is 100 × (body fluid outflow amount)
[g]) / (whole weight of silkworm before treatment [g]).

[0062]

[Table 2]

As can be seen from Table 2, a high outflow rate is shown under any conditions. From this, it is understood that bodily fluids can be efficiently collected by piercing the silkworm with infrared laser light. However, when the infrared laser light was irradiated under a strong condition such as 0.3 seconds at 10 W, the outflow of the body fluid was reduced, and even when the output of the infrared laser light was the same, it was 0.3 seconds. Even when irradiation is performed for a relatively long time, the outflow of bodily fluid is reduced. For this reason, it is preferable to appropriately control the output and irradiation time of the infrared laser beam and perform the perforation treatment under the condition that the body fluid can be collected most efficiently.

On the other hand, in comparison with this, when a mini drill having a diameter of 0.7 mm was rotated at 7000 rpm to pierce the star-shaped pattern of the silkworm, the outflow rate was 0.73 g on average for 6 animals and 1
2.7%. However, the time required for perforation is 1 second or more, and it is necessary to remove tissues or the like clogged in the groove of the mini-drill after treating two or three silkworms. Reaching frequently also occurred. For this reason, in the technique of mechanically piercing using a mini-drill, the treatment efficiency is significantly reduced, and it is also difficult to collect only pure body fluid.

Example 3 In Example 3, silkworm nuclear polyhedrosis virus (hereinafter referred to as NPV) was obtained after perforating silkworms.
Was inoculated, and an experiment was conducted to examine the incidence of NPV. The silkworm used in this experiment was a 5-year-old silkworm called "BCSL
1 × BNL1 ”and pupa“ Shin Asagiri ”. The inoculated NPV was -logLD _{50 in} the centrifuged supernatant of the diseased silkworm body fluid.
The value is 5.50. In this experiment, the output of the infrared laser light was 3 W, and the irradiation time was 0.1 second. Also,
At the time of inoculation of NPV, a method of coating with absorbent cotton impregnated with an NPV solution and a method of immersing in NPV solution for 3 minutes were adopted. Table 3 shows the results.

[0066]

[Table 3]

As shown in Table 3, regardless of the number of times of irradiation of the infrared laser beam, once or twice, each variety shows a high disease incidence. In particular, in the case of pupae, the disease incidence is high.

[0068]

As described above in detail, the insect incision cutting apparatus according to the present invention cuts and / or pierces the outer skin of an insect using infrared laser light by means of infrared irradiation means. Therefore, according to the present invention, it is possible to accurately incise and / or pierce only the outer coat of the insect without unnecessarily damaging the insect to be treated.

In the insect skin incision method according to the present invention, the insect skin is cut and / or pierced by irradiating the insect skin with infrared laser light. For this reason, according to the present invention, it is possible to quickly and accurately incise and / or pierce only the outer coat of the insect without unnecessarily damaging the insect to be treated.

[Brief description of the drawings]

FIG. 1 is a schematic configuration diagram of an insect incision apparatus shown as an example of the present invention.

FIG. 2 is a schematic structural diagram of an insect incision apparatus shown as another example of the present invention.

FIG. 3 is a schematic configuration diagram of an insect incision apparatus shown as another example of the present invention.

[Explanation of symbols]

1 belt conveyor, 2 belt section, 3 mounting table, 4
Silkworm supply unit, 5 position detection sensor, 6 laser irradiation device, 7 silkworm recovery unit

Claims (4)

(57) [Claims] 1. A mounting table for fixing and holding an insect to be treated, laser irradiation means for irradiating an infrared laser beam to the insect held on the mounting table, and moving the mounting table in a predetermined direction. A moving means for controlling, and a position for detecting a position of an insect held on the mounting table, which is disposed on a stage preceding the laser irradiation means with respect to a moving direction of the mounting table moved by the moving means. Detecting means, and inputting the position signal of the insect detected by the position detecting means to the laser irradiating means, and irradiating infrared laser light to the insect detected by the laser irradiating means based on the position signal. An insect skin incision apparatus characterized by incising and / or piercing an insect skin held by the mounting table. 2. A mounting table for holding an insect to be treated fixedly having negative pressure generating means for generating a negative pressure for holding the insect, and an infrared laser for the insect held on the mounting table. Laser irradiation means for irradiating light, by holding the insects on the mounting table by the negative pressure generated by the negative pressure generating means, in a state where the insects are suspended vertically downward, the laser irradiation means An insect skin incision apparatus, wherein the insect skin is incised and / or pierced by irradiating the insect with infrared laser light. 3. An insect to be treated is held on a mounting table, the position of the insect held on the mounting table is detected, and the detected insect is irradiated with infrared laser light based on the position information of the insect. Incising the hull of the insect and / or
Or a method for insect skin incision, characterized by perforating. 4. A negative pressure is applied to an insect to be treated, whereby the insect is suspended vertically and held on a mounting table, and an infrared laser beam is applied to the insect suspended vertically. A method for insect skin incision, characterized by incising and / or perforating the outer skin of the insect by irradiating in the midline direction.