JP3299832B2 - Analysis equipment - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an analyzer, and more particularly, to an analyzer for sampling a liquid sample such as blood contained in a sample container and performing a predetermined analysis. .

[0002]

2. Description of the Related Art Conventionally, this type of analyzer has a so-called disposable type for the purpose of preventing cross-contamination of a sample such as serum contained in a sample container when the sample is sampled by a pipette for aspirating a sample. (Disposable) pipettes are widely used.

[0003] This disposable pipette is usually
Made from plastics such as polypropylene. For this reason, in consideration of moldability and the like while securing a certain strength, the diameter and the wall pressure are made larger than those of a metal pipette. Also, the inside of the disposable pipette is empty, and the sample will be aspirated through its large volume of air.

[0004]

When a trace amount (for example, several microliters) of a sample is to be determined using the disposable pipette as described above, as described above, a large diameter or a large wall pressure or a large air space is required. Therefore, there was a problem that it was not possible to secure sufficiently high precision for the quantification.

The present invention has been made in view of the above circumstances, and has as its object to prevent contamination of a sample by a pipette at the time of sampling and to ensure sufficiently high precision in trace amount determination of a sample. It is an object of the present invention to provide an analyzer capable of performing the following.

[0006]

SUMMARY OF THE INVENTION An analyzer according to the present invention comprises a sample container for storing a liquid sample such as blood (serum, plasma, etc.) or body fluid such as urine to be analyzed, and a sample container for storing the sample. A disposable pipette that discharges the sample that has been suctioned in a larger amount than the analysis required, and then discharges the sample that has been suctioned and dispensed, and temporarily stores the sample that is discharged from the disposable pipette and temporarily stores it. The storage unit, the sample stored in the temporary storage unit is aspirated and collected by a required amount for analysis, and then the pipette for trace quantification that discharges the sample collected by suction and the sample discharged from the pipette for trace quantification are collected. And an analysis container for receiving the sample and performing a predetermined analysis.

As a sample container, for example, a test tubular container is used. As the disposable pipette, for example, a pipe made of a plastic such as polypropylene is used. For example, pipettes made of stainless steel, other alloys, and metals are used as microtiter pipettes. As the analysis container, for example, a test tube-shaped container is used.

As the temporary storage section, for example, a reaction container or a film or sheet arranged in a belt shape is preferably used. It is preferable to use a disposable type reaction vessel, film or sheet. Examples of films or sheets include plastic films,
A single sheet of paper, cloth, or the like, or a composite sheet obtained by appropriately laminating them is used. The material of the surface of the film or sheet that receives the sample can absorb the sample,
A sample that does not react with the sample is selected. Such a film or sheet is, for example, gradually pulled out from a roll and supplied.

[0009]

The disposable pipette aspirates and dispenses a sample contained in a sample container in a larger amount than required for a predetermined analysis. Next, the sample sampled by suction is discharged to the temporary storage unit. The temporary storage unit receives the sample discharged from the disposable pipette and temporarily stores the sample. The microquantitative pipette aspirates and dispenses the sample stored in the temporary storage unit with high accuracy by an amount necessary for a predetermined analysis.
Next, the sample sampled by suction is discharged into an analysis container.

[0010]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail based on three embodiments shown in the drawings. The invention is not limited by these.

[0011] In Example 1 1, the analysis apparatuses D ₁ according to Example 1, a sample container 1, and disposable pipette 2, the reaction vessel 3 as the temporary storage unit, a microtiter pipette 4, analysis container 5 is provided.

The sample container 1 is a test tube-shaped transparent container made of polyethylene. This sample container 1 contains blood for analysis (for example, serum) s as a liquid sample. The disposable pipette 2 is a translucent body made of polypropylene, aspirates blood s stored in the sample container 1 in a larger amount than necessary for a predetermined analysis, and then reacts the blood s aspirated and collected. Discharge into container 3.

The reaction container 3 is a transparent container made of polyethylene, and receives the blood s discharged from the disposable pipette 2 and temporarily stores it. The microquantitative pipette 4 is a hollow needle-like body made of stainless steel, and aspirates and dispenses the blood s stored in the reaction container 3 with a high precision necessary for a predetermined analysis. Blood s
Is discharged into the analysis container 5. The analysis container 5 receives the blood s discharged from the microquantitative pipette 4 and provides it for analysis.

[0014] Next, the analysis device D _1, the procedures up for analysis will be described. First, the collected blood for analysis s is stored in the sample container 1. And the sample container 1
The blood s stored in the sample is aspirated and collected by the disposable pipette 2 in an amount larger than that required for a predetermined analysis. Next, the blood s sucked and collected by the disposable pipette 2 is discharged to the reaction container 3. Blood s is reaction container 3
Is temporarily stored. When dispensing serum from another sample container, the disposable pipette 2 is replaced.

Thereafter, the microquantitative pipette 4 aspirates and dispenses the blood s stored in the reaction vessel 3 by an amount necessary for a predetermined analysis and a necessary number of times with high precision. Next, the aspirated and collected micro blood s is discharged to one or more analysis containers 5. Also, by using the microquantitative pipette 4 (of course, another microquantitative pipette may be used),
The reagent collected in a trace amount is discharged to the analysis container 5. The mixed solution having undergone a predetermined reaction in the analysis container 5 is subjected to a predetermined analysis.

In FIG. 1, a black arrow indicates suction of blood s by the disposable pipette 2 or the microquantitative pipette 4, and a white arrow indicates discharge of blood s by the disposable pipette 2 or the microquantitative pipette 4. .

The analyzer D ₁ aspirates and dispenses a sample container 1 in which blood s as a sample to be analyzed is stored and blood s stored in the sample container 1 in a larger amount than required for analysis.
Next, a disposable pipette 2 for discharging the blood s collected by suction, a reaction container 3 as a temporary storage unit for receiving and temporarily storing the blood s discharged from the disposable pipette 2, and a reaction container 3 The blood s stored in the pipette is aspirated and collected by a required amount, and then the pipette 4 for microquantitative discharge which discharges the blood s collected and aspirated and the blood s discharged from the pipette 4 for microquantitative receive and receive it. And an analysis container 5 for performing a predetermined analysis. Therefore, according to the analysis device D _1, so replace the disposable pipette 2 for each sample container 1 is performing the sampling of blood s, causing cross-contamination of samples other samples is mixed into the sample container 1 There is no such thing. Further, since the microquantitative pipette 4 is used for microquantification of blood s, sufficiently high precision can be secured.

Example 2 In FIG. 2, an analyzer D _{2 according} to Example ₂ comprises a sample container 1, a disposable pipette 2, a pipette 4 for trace quantification, and an analyzer container, similarly to the analyzer D _{1 according} to Example 1. 5 (both not shown), and a sheet 13 arranged in a belt shape as a temporary storage unit.

The sheet 13 is wound in a roll shape, is rotatably mounted on two left and right parallel rotating shafts, is disposed substantially horizontally, and is gradually drawn from left to right. I have. The surface on the front side (upper surface) of the sheet 13 is provided with a predetermined coating so that there is no possibility of absorbing the blood s as a sample to be analyzed or reacting with the blood s. Further, a plurality of convex portions 14 are provided so that the liquid sample can be suitably held at a predetermined position. A concave portion may be provided instead of the convex portion 14.

Next, a description will be given of a procedure until the analyzer D ₂ performs analysis. First, the blood for analysis s is collected and stored in the sample container 1. And the sample container 1
The blood s stored in the sample is aspirated and collected by the disposable pipette 2 in an amount larger than that required for a predetermined analysis. Next, the blood s sucked and collected by the disposable pipette 2 is discharged onto the sheet 13. Blood s sheet 13
Is temporarily stored on the upper surface of the

Thereafter, the microquantitative pipette 4 aspirates and dispenses the blood s stored on the upper surface of the sheet 13 only in an amount required for a predetermined analysis and a required number of times with high precision. Next, the blood s that has been suctioned and collected is discharged to the analysis container 5. A trace amount of the reagent is also dispensed into the analysis container 5.
The mixed solution is subjected to a predetermined analysis by the analysis container 5 and the microquantitative pipette 4.

The analyzer D ₂ aspirates and dispenses a sample container 1 containing blood s as a sample to be analyzed and blood s contained in the sample container 1 in a larger amount than required for analysis.
Next, a disposable pipette 2 for discharging the blood s collected by suction, a sheet 13 as a temporary storage unit for receiving and temporarily storing the blood s discharged from the disposable pipette 2, and an upper surface of the sheet 13. A small amount of pipette 4 for aspirating and collecting the blood s stored in the chamber by a required amount for analysis and then discharging the blood s collected and aspirated.
And an analysis container 5 for receiving the blood s discharged from the microquantitative pipette 4 and subjecting it to a predetermined analysis. Therefore, according to the analyzer D ₂ , it is possible to prevent the cross-contamination of the sample in the sample container 1, and it is possible to secure a sufficiently high precision for the trace amount determination of the blood s.

Third Embodiment In FIG. 3, an analyzer D _{3 according} to a _third embodiment includes a sample container 1, a disposable pipette 2, a microquantitative pipette 4, and an analysis container similarly to the analyzer D _{1 according} to the first embodiment. 5 (both not shown), and additionally, a belt-like film 23 as a temporary storage unit.

The film 23 is made of a plastic film wound in a roll shape, is rotatably mounted on two left and right parallel rotating shafts, is disposed substantially horizontally, and is gradually pulled out from left to right. It has become. Under the substantially horizontal portion of the film 23, a support 24 is disposed substantially horizontally. The support 24 has a film 23 on its upper surface.
I support.

As shown in FIG. 4, the support 24 has a rectangular shape whose plan shape is long on the left and right. In the left part of the support 24, a circular recess 24a is provided on the upper surface. The center of the recess 24a communicates with a through hole 24b penetrating from the upper surface to the lower surface of the support 24.

An air suction tube 25 is provided below the support 24. That is, the tube 25 is attached to the joint member 26 attached to the lower opening of the through hole 24 b of the support 24. The tube 25 is connected to an air suction pump (not shown). When the pump operates, a negative pressure (a pressure lower than the atmospheric pressure) is generated in the tube 25, the joint member 26, and the through hole 24b. Then, in the film 23 supported by the support 24, a substantially circular recess 23a is formed at a position corresponding to the recess 24a of the support 24. Thereby, the blood s as a sample to be analyzed can be reliably stored in the concave portion 23a of the film 23.

Next, a description will be given of a procedure until the analyzer D ₃ performs analysis. First, the blood for analysis s is collected and stored in the sample container 1. And the sample container 1
The blood s stored in the sample is aspirated and collected by the disposable pipette 2 in an amount larger than that required for a predetermined analysis. Next, the blood s sucked and collected by the disposable pipette 2 is discharged into the concave portion 23 a of the film 23. Blood s is temporarily stored in recess 23 a of film 23.
Then, a predetermined reagent is put into the blood s in the concave portion 23a of the film 23 and reacted with the blood s.

Thereafter, the microquantitative pipette 4 aspirates and dispenses the blood s stored in the concave portion 23a of the film 23 by an amount necessary for a predetermined analysis with high precision. Next, the blood s that has been suctioned and collected is discharged to the analysis container 5. Analysis container 5
Receives the blood s discharged from the microquantitative pipette 4 and supplies it to a predetermined analysis.

The analyzer D ₃ aspirates and dispenses a sample container 1 containing blood s as a sample to be analyzed and blood s contained in the sample container 1 in a larger amount than required for analysis.
Next, a disposable pipette 2 for discharging the blood s collected by suction, a film 23 as a temporary storage unit for receiving and temporarily storing the blood s discharged from the disposable pipette 2, and a concave portion of the film 23. The blood s stored in 23a is aspirated and collected by an amount required for analysis, and then the micro quantitation pipette 4 for discharging the aspirated blood s and the blood s discharged from the micro quantification pipette 4 are received. And an analysis container 5 for performing a predetermined analysis. Therefore, according to the analyzer D ₃ , it is possible to prevent cross-contamination of the sample in the sample container 1, and to secure sufficiently high precision for the trace amount determination of the blood s.

[0030]

Since the analyzer according to the present invention is configured as described above, it is possible to prevent contamination of the sample by the pipette at the time of sampling, and to secure sufficiently high precision in the trace amount determination of blood s. be able to.

[Brief description of the drawings]

FIG. 1 is an explanatory diagram of a main part configuration of an analyzer according to a first embodiment of the present invention.

FIG. 2 is an explanatory diagram of a main part configuration of an analyzer according to a second embodiment of the present invention.

FIG. 3 is an explanatory diagram of a main part configuration of an analyzer according to a third embodiment of the present invention.

FIG. 4 is a plan view of a main part of an analyzer according to a third embodiment.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Sample container 2 Disposable pipette 3 Reaction container (temporary storage part) 4 Pipette for trace amount determination 5 Analysis container 13 Sheet (temporary storage part) 23 Film (temporary storage part)

──────────────────────────────────────────────────続 き Continued on the front page (58) Field surveyed (Int.Cl. ⁷ , DB name) G01N 35/10 G01N 1/00 101 G01N 1/10 G01N 1/14 G01N 33/48

Claims (3)

(57) [Claims] 1. A sample container in which a liquid sample such as blood to be analyzed is stored, and a sample stored in the sample container is aspirated and collected in a larger amount than required for analysis. A disposable pipette to be discharged, a temporary storage unit for temporarily storing a sample discharged from the disposable pipette and temporarily storing the sample, and aspirating and collecting a required amount of the sample stored in the temporary storage unit, Next, an analysis device comprising: a microquantitative pipette for discharging the sample aspirated and dispensed; and an analysis container for receiving the sample discharged from the microquantitative pipette and subjecting it to a predetermined analysis. 2. The analyzer according to claim 1, wherein the temporary storage section is a reaction vessel. 3. The analyzer according to claim 1, wherein the temporary storage section is a film or a sheet arranged in a belt shape.