JP3162273U - Centrifugal filter - Google Patents

Abstract

Disclosed is a centrifugal filter capable of easily and reliably separating a liquid or solid from a dispersed or suspended solution in a minitube set in a centrifuge. A centrifugal filter used by being fitted between an upper portion of a mini tube and a lower portion of the mini tube set in a centrifuge, wherein the centrifugal filter is disposed between the upper portion of the mini tube and the lower portion of the mini tube. A thin filter paper that allows only liquid to pass in the direction and an inner peripheral surface for supporting the filter paper are constituted by a cylindrical housing unit, and the filter paper is provided between an upper end portion and a lower end portion of the housing unit. The upper end portion and the lower end portion of the housing unit have the same shape and size having a circular opening. [Selection] Figure 2

Description

  The present invention relates to a centrifugal filter for separating liquid and solid by centrifugation when it is necessary to separate liquid and solid in a solution in a minitube.

Conventionally, in order to separate a liquid and a solid in a solution dispersed or suspended in a minitube by filtration, the following method is performed.
First, in order to perform filtration, a filter paper and a funnel are separately prepared, and the dispersion or suspension solution in the minitube is taken out of the system to perform filtration.
Second, a so-called coma filter containing a membrane filter is attached to a syringe, and the suspension solution is transferred into the syringe, followed by filtration by pressurization or decompression.
However, these methods have a problem that the dispersion or suspension solution in the minitube needs to be taken out once.

Therefore, a method of separating the liquid phase from the solid phase is performed by centrifuging by setting a mini tube in a centrifuge, which is a device that separates liquid and solid using centrifugal force, and rotating at high speed. It has been broken.
As the minitube set in the centrifuge in this case, for example, a minitube having a shallow cylindrical recess provided on the bottom surface of the minitube and a two-dimensional barcode attached to the ceiling surface of the recess (see Patent Document 1) ) And a test suitable for a small centrifuge that has a smaller inner diameter than the inner body diameter, a narrow bottom, and a thread on the outer periphery of the mouth so that the cap can be screwed on. Tubes (see Patent Document 2) are provided.

Japanese Patent No. 4168091 Utility Model Registration No. 3070056

  However, in these minitubes, although the liquid and solid can be separated once in the minitube, in order to actually take the liquid phase and / or solid phase (or both) required for the subsequent operation, usually the upper layer is used. A liquid phase needs to be taken out by using a pipette or the like. Further, at that time, the interface between the liquid phase and the solid phase is often disturbed, and there is a problem that the separation of the liquid and the solid is incomplete as compared with the above-described filtration method.

  In order to solve the above problems, a centrifugal filtration filter according to the present invention is a centrifugal filtration filter that is used by being fitted between a minitube upper part and a minitube lower part set in a centrifuge, The centrifugal filter is composed of a thin filter paper that allows only liquid to pass from the top of the minitube to the bottom of the minitube, and an inner peripheral surface for supporting the filter paper, and a cylindrical housing unit. The filter paper is disposed between the upper end portion and the lower end portion of the housing unit, and the upper end portion and the lower end portion of the housing unit have the same shape and size having a circular opening.

  A housing unit of the centrifugal filter is composed of an upper part and a lower part, and the upper part and the lower part are welded with the filter paper interposed therebetween.

  A housing unit of the centrifugal filter includes an upper part and a lower part, and the upper part and the lower part are fitted with the filter paper interposed therebetween.

  The housing unit of the centrifugal filter and the filter paper have an integral structure by insert molding.

  A part of the lower part of the housing unit has a circular flat plate-like structure so as to contact the lower side of the filter paper, and a plurality of through holes for allowing liquid to pass through the circular flat plate-like structure portion. It is provided.

  A part of each of the upper part and the lower part of the housing unit has a circular flat plate structure so as to contact the upper side and the lower side of the filter paper, and each of the circular flat plate structure parts has the same position. A plurality of through holes for allowing liquid to pass therethrough are provided.

  A detachable circular flat support member having a plurality of through holes for allowing liquid to pass is attached between the filter paper and the lower part of the housing unit.

  Removable circular flat support parts having a plurality of through holes for allowing liquid to pass through the same position are attached between the filter paper and the lower part of the housing unit and between the filter paper and the upper part of the housing unit, respectively. It is characterized by being.

  A part of the housing unit has a circular flat plate structure so as to contact the lower side of the filter paper, and the circular flat plate structure portion is provided with a plurality of through holes for allowing liquid to pass. It is characterized by.

  A part of the housing unit has a circular flat plate-like structure so as to contact the upper side and the lower side of the filter paper, and each of the circular flat plate-like structural parts is used for passing liquid to the same position. A plurality of through holes are provided.

First, according to the present invention, a dispersed or suspended solution can be easily and reliably separated into a liquid and a solid while still in a minitube set in a centrifuge.
Secondly, according to the present invention, the filtration operation can be performed in a closed system, so that exposure to the experimenter can be reduced when handling harmful environmental pollutants or microbial suspensions. It becomes possible.
Third, the mini-tube used in the upper part and lower part of the mini-tube of the present invention is not limited to the one dedicated to the present invention, and the existing mini-tube with the lid part removed can be used as it is. Therefore, it can be implemented at low cost.

It is explanatory drawing of the minitube of the state to which the centrifugal filter concerning this application was attached. 1 is a longitudinal sectional view of a centrifugal filter formed by welding in Example 1. FIG. 1 is a longitudinal sectional view of a centrifugal filtration filter formed by fitting in Example 1. FIG. 1 is a longitudinal sectional view of a centrifugal filter formed by integral molding in Example 1. FIG. It is a longitudinal cross-sectional view of the centrifugal filter in which the lower part of the housing unit in Example 2 has a circular flat plate-like structure. It is a longitudinal cross-sectional view of the centrifugal filter in which the upper part and the lower part of the housing unit in Example 2 have a circular flat plate-like structure. 6 is a plan view of a centrifugal filter in Example 2. FIG. It is a longitudinal cross-sectional view of a centrifugal filtration filter when one supporting component having a through hole in Example 3 is attached. It is a longitudinal cross-sectional view of the centrifugal filtration filter when the two support components which have a through-hole in Example 3 are attached.

  The objective of the present invention is to provide a centrifugal filter that can easily and reliably separate a dispersion or suspension solution into a liquid and a solid in a minitube set in a centrifuge. This was realized by a centrifugal filtration filter that was fitted between the upper part and the lower part and included a filter paper that allowed only liquid to pass from the upper part to the lower part of the minitube.

Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.
FIG. 1 is an explanatory diagram of a minitube set in a centrifuge with a centrifugal filter 3 attached thereto.
As described above, the centrifugal filter 3 according to the present invention is located between the minitube upper portion 1 and the minitube lower portion 2 and is used by being fitted to the minitube upper portion 1 and the minitube lower portion 2. And the centrifugal filter 3 is comprised from the thin plate-shaped filter paper 4 and the housing unit which includes the said filter paper like FIG. 2, The upper end part and lower end part of a housing unit are the same shape which has a circular opening part. And size. The filter paper 4 is disposed between the upper end portion and the lower end portion of the housing unit.

The mini-tube is usually composed of a main body that is a thin bottomed cylindrical container and a lid that is fitted into an opening at the top of the main body. Both the mini-tube upper part 1 and the mini-tube lower part 2 used in the present invention can use the main body part excluding the cover part of a normal mini-tube as it is. Thus, the minitube used for the minitube upper portion 1 and the minitube lower portion 2 is not limited to the one dedicated to the present invention, and can be used as it is without the lid portion of the existing minitube. Therefore, it can be carried out at a low cost.
Further, the mini-tube upper part 1 and the mini-tube lower part 2 do not have to have the same shape. It is sufficient if the inner diameter matches.

  The maximum outer diameter of the centrifugal filter 3 is not limited to the same as the maximum outer diameter of the minitube, and may be larger than the maximum outer diameter of the minitube. Thereby, it becomes easy to fit the centrifugal filtration filter 3 to the mini-tube upper part 1 and the mini-tube lower part 2 and to detach from the mini-tube upper part 1 and the mini-tube lower part 2.

A method of filtering using the centrifugal filter 3 according to the present invention will be described.
For example, when separating a liquid and a solid in a solution dispersed or suspended in a 2 mL volume mini-tube, first prepare an upper portion 1 of the mini-tube containing the solution with the opening facing upward, according to the present invention. The centrifugal filter 3 is fitted. Next, the mini-tube lower part 2 is fitted to the centrifugal filter 3 with the opening part facing down. Then, with the top and bottom turned upside down, the minitube upper part 1 is placed on the top and the minitube lower part 2 is placed on the bottom. As a result, the solid remains in the upper portion 1 of the minitube, and the liquid is filtered out in the lower portion 2 of the minitube.

  Various materials such as glass, polypropylene, and polystyrene can be used as the material of the minitube.

  The material of the housing unit of the centrifugal filter 3 may be any material that can be securely fitted to the minitube, and various materials such as polyethylene, polypropylene, and polystyrene are possible.

  The filter paper 4 can be made of various materials such as cotton fiber and glass fiber, and a paper with an appropriate fineness is selected according to the purpose of filtration.

  Next, the structure of the centrifugal filter 3 according to the present invention will be described. The housing unit may be in any case, such as a case where the housing unit containing the filter paper 4 is welded, a case where the housing unit is fitted, or a case where the housing unit is formed by integral molding.

  First, FIG. 2 is a longitudinal sectional view of a centrifugal filter 3 in which a housing unit is formed by welding. As described above, the housing unit of the centrifugal filter 3 is divided into the upper part 6 and the lower part 7, and the upper part 6 and the lower part 7 are welded with the filter paper 4 interposed therebetween. Yes.

  Next, FIG. 3 is a longitudinal sectional view of the centrifugal filter 3 in which the housing unit is formed by fitting. As described above, the housing unit of the centrifugal filter 3 is divided into the upper part 6 and the lower part 7, and the upper part 6 and the lower part 7 are fitted with the filter paper 4 interposed therebetween. ing.

  FIG. 4 is a longitudinal sectional view of the filtration filter 3 in which the housing unit and the filter paper 4 have an integral structure formed by insert molding. As described above, the housing unit of the centrifugal filter 3 is not limited to the one divided into the upper part 6 and the lower part 7, and the housing unit 5 having an integral structure including the filter paper 4 as shown in FIG. 4. It may be.

  In the case of the present embodiment, any of the welding in FIG. 2, the fitting in FIG. 3, and the integrated structure in FIG. Furthermore, in the case of the fitting shown in FIG. 3, it is possible to replace only the filter paper 4 by separating the upper part 6 and the lower part 7 of the housing unit.

FIG. 5 shows the centrifugal filter 3 used for the minitube set in the centrifuge of the second embodiment. In this embodiment, unlike the first embodiment, in order to prevent the filter paper 4 from falling out without resisting the weight of solid or liquid during centrifugation, the lower portion 7 of the housing unit is brought into contact with the lower side of the filter paper 4. A part of has a circular flat plate-like structure. The circular flat plate-shaped structural portion is provided with a plurality of through holes 8 for allowing liquid to pass therethrough, and the circular flat plate structure other than the through holes 8 allows the liquid to pass through the through holes 8. The portion prevents the filter paper 4 from falling off. Further, the upper part 6 and the lower part 7 of the housing unit are welded in the same manner as in the case of FIG.
It is not limited to the case where a part of the lower part 7 of the housing unit has a circular flat plate structure, and further, a part of the upper part 6 of the housing unit is located above the filter paper 4 as shown in FIG. It may be a case where a circular flat plate-like structure is provided so as to abut against each other, and a plurality of through holes 8 for allowing liquid to pass through are provided in the circular flat plate-like structure portion. In this case, it is assumed that a plurality of through holes 8 are provided in each of the circular flat plate-like structural portions of the upper part 6 and the lower part 7 of the housing unit for allowing liquid to pass through the same position.
FIG. 7 is a plan view of FIG. A circular flat plate-like structure is formed in the upper part 6 of the housing unit and abuts against the filter paper 4, and a through-hole 8 for passing a liquid is provided in the circular flat plate-like structure portion. The number of through holes 8 is not limited to three as shown in FIG.
In the case of this welding, when the portions abutting on the lower side and the upper side of the filter paper 4 each have a circular flat plate structure, the same operation can be performed even if the centrifugal filter 3 is turned upside down.

Further, the structure of the housing unit is not limited to the case of welding as shown in FIGS. 5 and 6, and may be a case of fitting. The housing unit of the centrifugal filter 3 in this case is also divided into an upper part 6 and a lower part 7, and a part of the lower part 7 of the housing unit is circular so as to contact the lower side of the filter paper 4. A flat plate-like structure is formed, and a plurality of through holes 8 for allowing liquid to pass therethrough are provided in the circular flat plate-like structure portion. Then, the upper part 6 and the lower part 7 of the housing unit are structured to be fitted with the filter paper 4 interposed therebetween. Then, the liquid is passed through the through-hole 8 and the circular flat plate-like structural portion other than the through-hole 8 prevents the filter paper 4 from falling out without resisting the weight of the solid or liquid during centrifugation. .
It is not limited to the case where a circular flat plate-like structure is formed in the lower part 7 of the housing unit. Furthermore, a circular flat plate-like structure is also formed in the upper part 6 of the housing unit, The case where the some through-hole 8 for letting a liquid pass to a circular flat plate-shaped structure part may be provided. In this case, it is assumed that a plurality of through holes 8 are provided in each of the circular flat plate-like structural portions of the upper part 6 and the lower part 7 of the housing unit for allowing liquid to pass through the same position. In this case, it is possible to replace only the filter paper 4 by separating the upper part 6 and the lower part 7 of the housing unit. Further, when both the lower and upper portions of the filter paper 4 have a circular flat plate structure, the same operation can be performed even if the centrifugal filter 3 is turned upside down.

Furthermore, the structure of the housing unit is not limited to the case where the housing unit is divided into the upper part 6 and the lower part 7, and the housing unit and the filter paper 4 may be an integral structure by insert molding. For example, even if the housing unit 5 is integrally molded and has a circular plate-like structure in which a plurality of through holes 8 for allowing liquid to pass therethrough so as to contact the lower side of the filter paper 4. good. Then, the liquid is passed through the through-hole 8 and the circular flat plate-like structural portion other than the through-hole 8 prevents the filter paper 4 from falling out without resisting the weight of the solid or liquid during centrifugation. .
Further, the present invention is not limited to the case where the circular flat plate-like structure is formed so as to be in contact with the lower side of the filter paper 4, and the circular flat plate-like structure is also provided so as to be in contact with the upper side of the filter paper 4. May be formed. In this case, it is assumed that each of the circular flat plate-like structural portions is provided with a plurality of through holes 8 through which liquid passes at the same position.
In the case of this integrated structure, if both the lower and upper portions of the filter paper 4 are in a circular flat plate structure, the centrifugal filter 3 can be similarly turned upside down.

FIG. 8 shows the centrifugal filter 3 used in the minitube set in the centrifuge of the third embodiment. The housing unit is divided into an upper part 6 and a lower part 7, and the upper part 6 and the lower part 7 have a structure in which the filter paper 4 and the support component 9 are sandwiched therebetween. The support component 9 is a circular plate-shaped component, and a plurality of through holes 8 for allowing liquid to pass therethrough are provided. As described above, in the present embodiment, unlike the second embodiment, the plurality of through holes 8 through which the liquid passes are provided not in the housing unit but in the support component 9 that can be detached from the housing unit. A support component 9 is attached between the filter paper 4 and the lower part 7 of the housing unit, and is in contact with the lower side of the filter paper 4. Then, the liquid is passed through the through-hole 8 and the part of the support component 9 other than the through-hole 8 prevents the filter paper 4 from falling out without resisting the weight of the solid or liquid during centrifugation.
However, the support component 9 is not limited to the case where it is attached between the filter paper 4 and the lower part 7 of the housing unit as shown in FIG. It may be attached in between and may be in contact with the upper side of the filter paper 4. In this case, it is assumed that a plurality of through-holes 8 are provided in each of the support components 9 that are in contact with the upper side and the lower side of the filter paper 4 to allow liquid to pass through the same position.
The support component 9 may be made of any material as long as the filter paper 4 is strong enough to prevent the filter paper 4 from falling out without resisting the weight of the solid or liquid during the centrifugation, and may be polyethylene, polypropylene, polystyrene, metal, glass, and the like. Various things are possible.

  In the case of this embodiment, it is possible to replace only the filter paper 4 by separating the upper part 6 and the lower part 7 of the housing unit. Further, when the support component 9 is disposed above and below the filter paper 4, it can be similarly implemented even if the centrifugal filter 3 is turned upside down.

DESCRIPTION OF SYMBOLS 1 Minitube upper part 2 Minitube lower part 3 Filtration filter 4 Filter paper 5 Housing unit 6 Upper part of housing unit 7 Lower part of housing unit 8 Through-hole 9 Supporting parts

Claims (10)

A centrifugal filter used by being fitted between an upper part of a mini-tube and a lower part of a mini-tube set in a centrifuge,
The centrifugal filter is
A thin plate-like filter paper that allows only liquid to pass from the upper part of the minitube to the lower part of the minitube and an inner peripheral surface for supporting the filter paper are constituted by a cylindrical housing unit,
The filter paper is disposed between an upper end portion and a lower end portion of the housing unit,
The centrifugal filter according to claim 1, wherein the upper end portion and the lower end portion of the housing unit have the same shape and size having a circular opening. 2. The centrifugal filter according to claim 1, wherein the housing unit of the centrifugal filter comprises an upper part and a lower part, and the upper part and the lower part are welded with the filter paper interposed therebetween. The centrifugal filter according to claim 1, wherein the housing unit of the centrifugal filter comprises an upper part and a lower part, and the upper part and the lower part are fitted with the filter paper interposed therebetween. The centrifugal filter according to claim 1, wherein the housing unit of the centrifugal filter and the filter paper have an integral structure formed by insert molding. A part of the lower part of the housing unit has a circular flat plate-like structure so as to contact the lower side of the filter paper, and a plurality of through holes for allowing liquid to pass through the circular flat plate-like structure portion. The centrifugal filter according to any one of claims 1 to 3, wherein the centrifugal filter is provided. A part of each of the upper part and the lower part of the housing unit has a circular flat plate-like structure so as to contact the upper side and the lower side of the filter paper, and the circular flat plate-like structural part has the same position. The centrifugal filtration filter according to any one of claims 1 to 3, wherein a plurality of through holes for allowing liquid to pass therethrough are provided. The centrifugal plate according to claim 3, wherein a detachable circular flat support member having a plurality of through holes for passing a liquid is attached between the filter paper and the lower part of the housing unit. Filtration filter. Removable circular plate-like support parts having a plurality of through holes for allowing liquid to pass through the same position are attached between the filter paper and the lower part of the housing unit and between the filter paper and the upper part of the housing unit, respectively. The centrifugal filter according to claim 3, wherein the centrifugal filter is provided. A part of the housing unit has a circular flat plate structure so as to contact the lower side of the filter paper, and the circular flat plate structure portion is provided with a plurality of through holes for allowing liquid to pass. The centrifugal filter according to claim 4, wherein A part of the housing unit has a circular flat plate-like structure so as to contact the upper side and the lower side of the filter paper, and each of the circular flat plate-like structural parts is used for passing liquid to the same position. The centrifugal filter according to claim 4, wherein a plurality of through holes are provided.

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