CN210646468U - Expandable ultrafiltration centrifuge tube using AAO porous membrane - Google Patents
Expandable ultrafiltration centrifuge tube using AAO porous membrane Download PDFInfo
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- CN210646468U CN210646468U CN201921145962.0U CN201921145962U CN210646468U CN 210646468 U CN210646468 U CN 210646468U CN 201921145962 U CN201921145962 U CN 201921145962U CN 210646468 U CN210646468 U CN 210646468U
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Abstract
The utility model discloses an extensible ultrafiltration centrifuge tube using AAO porous membrane, which comprises a cover, a filtrate cylinder, a plurality of filter layers and a bottom cylinder which are connected in sequence, wherein the filter layers comprise a sealing ring, the AAO porous membrane, a supporting sheet and a cascade piece which are tightly attached in sequence, and the filtrate cylinder is tightly attached to the sealing ring in the adjacent filter layer; the cascade part of the filter layer is tightly attached to the sealing ring in the adjacent filter layer; the bottom cylinder is connected with the cascade piece in the filter layer adjacent to the bottom cylinder. The utility model discloses utilize the AAO porous membrane as the milipore filter, the aperture is even, and the filter effect is accurate reliable, simultaneously, the utility model provides a filter layer is expanded, can realize the layer upon layer separation to different particle diameter granule, can reach the while and realize multistage filtration, can obtain the granule of a certain middle particle diameter again accurately. Additionally, the utility model discloses simple structure, simple to operate, each part is inseparable through connecting, can prevent effectively that the filtrate from revealing.
Description
Technical Field
The utility model relates to an ultrafiltration centrifuging tube preparation technical field, especially use the expanded ultrafiltration centrifuging tube of AAO (anodic aluminum oxide) porous membrane.
Background
Centrifugation techniques have found wide application in the field of biological science, particularly in the field of biochemical and molecular biological research. The centrifugal technology is mainly used for separating and preparing various biological samples, a biological sample suspension is placed in a centrifugal tube and rotates at a high speed, and suspended micro particles (such as organelles, the sediment of biological macromolecules and the like) are settled at a certain speed under the action of huge centrifugal force, so that the suspended micro particles and a solution are separated. Centrifuge tubes are important devices in centrifugal technology and are typically tubular containers with lids. The ultrafiltration centrifugal tube is provided with an ultrafiltration membrane in the centrifugal tube, and the ultrafiltration membrane can play a role in intercepting particles, so that the particles and solution can be quickly separated. At present, ultrafiltration membranes in ultrafiltration centrifuge tubes are mainly of four types, namely polyether sulfone, cellulose triacetate, regenerated cellulose and Hydrosart (an improved regenerated cellulose), and all of the four types have the problem of low aperture uniformity, particles with different particle sizes cannot be effectively separated in an ultrafiltration centrifugation process, so that the rejection rate of nanoparticles is not high, and the particles are difficult to be accurately separated.
In addition, in the field of fine filtration, a sample is often mixed with nanoparticles (such as 50 nanometers, 100 nanometers and 200 nanometers) with multiple sizes, and some applications require high-precision separation of particles within a certain middle size range. Therefore, it is necessary to develop an ultrafiltration centrifugal tube which can perform precise filtration and can perform multi-layer filtration.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an use expanded ultrafiltration centrifuging tube of AAO porous membrane, improve the filter effect of ultrafiltration centrifuging tube, effectively separate the granule of different particle diameters to can separate out certain intermediate size's granule from the solution that contains multiple size granule, can carry out the multi-stage filtration separation simultaneously.
In order to solve the technical problem, the utility model discloses a following technical scheme: use expanded ultrafiltration centrifuging tube of AAO porous membrane, including lid, a filtrate section of thick bamboo, a plurality of filter layer and the end section of thick bamboo that connects in order, the filter layer is including the sealing washer, the AAO porous membrane, the backing sheet and the cascade piece of closely laminating in order, the sealing washer in a filtrate section of thick bamboo and the filter layer adjacent with it closely laminates. The cascade piece of the filter layer is tightly attached to the sealing ring in the adjacent filter layer. The bottom cylinder is connected with the cascade piece in the filtering layer adjacent to the bottom cylinder.
The cover comprises a cover plate and a boss which are integrally formed and coaxially arranged, the cover plate is a circular thin plate, the boss is cylindrical, and an external thread is formed on the outer side surface of the boss.
The filtrate cylinder comprises a filtrate containing cavity and a first connecting head which are integrally formed and coaxially arranged, the filtrate containing cavity is a hollow cylinder, the inner diameter of the filtrate containing cavity is equal to the diameter of the boss, the inner side of one end, far away from the first connecting head, of the filtrate containing cavity is provided with an internal thread matched with the external thread of the outer side surface of the boss, and the filtrate containing cavity is in threaded connection with the boss. The first connecting head is a hollow cylinder, the outer diameter of the first connecting head is smaller than that of the filtrate containing cavity, and external threads are formed in the outer side of the first connecting head.
The utility model provides a cascade member includes connecting seat and the second connector of integrated into one piece and coaxial arrangement, the connecting seat is hollow cylinder, and the internal diameter of connecting seat equals with the external diameter of first connector, and the internal thread with first connector outside external screw thread assorted is seted up to the connecting seat inboard. The second connector is a hollow cylinder, the outer diameter of the second connector is equal to the inner diameter of the connecting seat, the outer side of the second connector is provided with an outer thread matched with the inner thread of the connecting seat, and the cascade parts in the adjacent filter layers are connected through threads.
The utility model provides a bottom cylinder is one end closed, and other end open-ended is cylindrical, and the inside circular cavity of having seted up of bottom cylinder for store through filterable solution, the internal diameter of bottom cylinder equals with the external diameter of second connector, and the inboard of bottom cylinder is seted up with second connector outside external screw thread assorted internal thread, bottom cylinder and adjacent filter layer threaded connection with it.
In many centrifugal experiments, the cleanliness requirement of centrifuge tubes is very high, and even disposable use is required, so that cross contamination is prevented. The utility model discloses the volume of cavity is greater than the volume of cavity in the filtrating section of thick bamboo in the end section of thick bamboo, can add same kind of filtrate in to the filtrating section of thick bamboo many times, can save the washing cost or the replacement cost of centrifuging tube to a certain extent.
The sealing ring is annular, the AAO porous membrane and the supporting sheet are circular, the diameters of the AAO porous membrane and the supporting sheet are equal to the inner diameter of the connecting seat, and the sealing ring, the AAO porous membrane and the supporting sheet are tightly attached to each other and arranged in the connecting seat of the cascade piece. The support sheet is a rigid wafer and plays a role in supporting and protecting, and the AAO porous membrane is clamped between the seal ring and the support sheet, so that the fragile AAO porous membrane can be protected, and the AAO porous membrane is prevented from being broken to cause filtration failure. The effect of backing sheet is protection AAO porous membrane, and simultaneously, the backing sheet can not block the filtrate circulation, consequently, the utility model provides a backing sheet middle part all is equipped with circular through-hole array, and the internal diameter within range of second connector is located to the border of seting up of circular through-hole array, and the backing sheet can enough provide the support for AAO porous membrane, can pass through the circular through-hole array in the middle of the backing sheet again, guarantees that the filtrate passes through smoothly.
The utility model provides an inside through-hole of a filtrating section of thick bamboo is the reducing through-hole, and the internal diameter that a filtrating section of thick bamboo is close to lid one end is the biggest, and a filtrating section of thick bamboo is close to the internal diameter of filter layer one end and is minimum, and a filtrating section of thick bamboo is close to the internal diameter of filter layer one end and is less than the internal diameter of sealing washer. One side of the axial section of the reducing through hole, which is close to the cover, is rectangular, one side of the axial section of the reducing through hole, which is close to the filter layer, is inverted trapezoidal, and the inner diameter of the reducing through hole is uniformly reduced. The internal diameter of reducing through-hole reduces in the filtrating section of thick bamboo to one end that is close to the filter layer at a filtrating section of thick bamboo reaches the minimum, is favorable to the filtrate to get into the filter layer smoothly, prevents between filtrate infiltration filtrating section of thick bamboo and the filter layer, and simultaneously, the internal diameter uniform variation of reducing through-hole in the filtrating section of thick bamboo can prevent that the filtrate from being detained in the reducing department of reducing through-hole, influences the filter effect.
The utility model provides a filter layer can set up a plurality ofly, connects through cascading between a plurality of filter layers. The pore diameters of the AAO porous membranes in the plurality of filtering layers are different, the pore diameter of the AAO porous membrane in the filtering layer close to the cover is large, the pore diameter of the AAO porous membrane in the filtering layer close to the bottom barrel is small, and the filtering liquid sequentially passes through the plurality of filtering layers to realize multistage filtering and obtain particles with required particle sizes.
Compared with the prior art, the utility model discloses an useful part lies in: the utility model provides an use expanded ultrafiltration centrifuging tube of AAO porous membrane utilizes the AAO porous membrane as the milipore filter, and the aperture is even, and the filter effect is accurate reliable, simultaneously, the utility model provides a filter layer is expandable, can realize the layer upon layer separation to different particle diameter granules, can enough realize multistage filtration simultaneously, can obtain the granule of a certain middle particle diameter again accurately. The utility model discloses simple structure, simple to operate, each part passes through screw thread zonulae occludens to set up the sealing washer, can prevent effectively that the filtrate from revealing, in addition, the utility model discloses can also save the washing cost or the replacement cost of centrifuging tube.
Drawings
Fig. 1 is a schematic structural diagram of a top view of the present invention;
fig. 2 is a schematic structural view of the bottom view of the present invention;
fig. 3 is a schematic view of the installation of the present invention;
fig. 4 is an axial cross-sectional view of the present invention;
FIG. 5 is a first electron micrograph of the AAO porous membrane filter particles in the filter layer adjacent to the lid of example 3;
FIG. 6 is a second electron micrograph of the AAO porous membrane filtrate particles in the filter layer adjacent to the lid of example 3;
FIG. 7 is a photograph III of an electron microscope showing the filter particles of the AAO porous membrane in the filter layer near the lid in example 3;
FIG. 8 is a fourth electron micrograph of AAO porous membrane filter particles in the filter layer near the lid of example 3;
FIG. 9 is a fifth electron micrograph of AAO porous membrane filter particles in the filter layer near the lid of example 3;
FIG. 10 is a photograph of a sixth electron microscope showing the AAO porous membrane filtrate particles in the filtration layer near the lid in example 3;
FIG. 11 is a first photograph of an electron microscope showing the filter particles of the AAO porous membrane in the filter layer near the base cartridge in example 3;
FIG. 12 is a second electron micrograph of the AAO porous membrane filter particles in the filtration layer near the base cartridge of example 3;
FIG. 13 is a photograph III of an electron microscope showing the filter particles of the AAO porous membrane in the filter layer near the base cartridge in example 3;
FIG. 14 is a fourth electron micrograph of AAO porous membrane filter particles in the filtration layer near the base cartridge of example 3.
The meaning of the reference numerals: 1-cover, 2-filtrate cylinder, 3-filter layer, 4-bottom cylinder, 5-sealing ring, 6-AAO porous membrane, 7-support sheet, 8-cascade piece, 9-cover plate, 10-boss, 11-filtrate cavity, 12-first connector, 13-connecting seat, 14-second connector.
The present invention will be further described with reference to the accompanying drawings and the detailed description.
Detailed Description
The cover 1 comprises a cover plate 9 and a boss 10 which are integrally formed and coaxially arranged, the cover plate 9 is a circular thin plate, the boss 10 is cylindrical, and an external thread is formed on the outer side surface of the boss 10 and is used for being connected with the filtrate cylinder 2.
Filtrate section of thick bamboo 2 includes that integrated into one piece and coaxial arrangement's filtrating holds chamber 11 and first connector 12, filtrating holds chamber 11 and is hollow cylinder, and the internal diameter that filtrating held chamber 11 equals with the diameter of boss 10, and filtrating holds the inboard of the one end that first connector 12 was kept away from in chamber 11 and offers the internal thread with boss 10 outside external screw thread assorted, and the filtrating in filtrate section of thick bamboo 2 holds chamber 11 and the boss 10 threaded connection in lid 1. The first connecting head 12 is a hollow cylinder, the outer diameter of the first connecting head 12 is smaller than that of the filtrate containing cavity 11, and the outer side of the first connecting head 12 is provided with an external thread for connecting with the filter layer 3.
The filter layer 3 comprises a sealing ring 5, an AAO porous membrane 6, a support sheet 7 and a cascade piece 8 which are tightly attached in sequence. The cascade piece 8 includes connecting seat 13 and the second connector 14 of integrated into one piece and coaxial arrangement, connecting seat 13 is hollow cylinder, and the internal diameter of connecting seat 13 equals with the external diameter of first connector 12, and connecting seat 13 inboard is seted up with first connector 12 outside external screw thread assorted internal thread, and connecting seat 13 of cascade piece 8 and the first connector 12 threaded connection in the filtrating section of thick bamboo 2. The second connector 14 is a hollow cylinder, and external threads are formed in the outer side of the second connector 14. The sealing ring 5 is annular, and AAO porous membrane 6 and backing sheet 7 are circular, and the diameter of AAO porous membrane 6 and backing sheet 7 equals the internal diameter of connecting seat 13, and sealing ring 5, AAO porous membrane 6 and backing sheet 7 closely laminate install in the connecting seat 13 of cascade 8, and sealing ring 5 can prevent that the filtrate from revealing, can also restrict AAO porous membrane 6, and sealing ring 5 and backing sheet 7 cooperate, protect AAO porous membrane 6. The support sheet 7 is required to support the AAO porous membrane 6 to prevent the AAO porous membrane 6 from being broken under pressure, and to ensure smooth passage of the filtrate, so that a circular through hole array is arranged in the middle of the support sheet 7 in this embodiment, an opening boundary of the circular through hole array is within an inner diameter range of the second connector 14, the support sheet 7 is made of a rigid material to protect the AAO porous membrane 6, and the circular through hole array in the middle of the support sheet 7 can be used for discharging the filtrate.
The end section of thick bamboo 4 in this embodiment is one end and seals, and other end open-ended is cylindrical, and the circular cavity has been seted up to end section of thick bamboo 4 is inside, and the internal diameter of end section of thick bamboo 4 equals with the external diameter of second connector 14, and the internal thread that matches with the external screw thread in the second connector 14 outside is seted up to the inboard of end section of thick bamboo 4, and end section of thick bamboo 4 and the second connector 14 threaded connection in the cascade member 8. In this embodiment, the sealing rings 5 of the filtrate cartridge 2 and the filter layer 3 are tightly attached to each other and are in threaded connection with the cascade member 8. The bottom tube 4 and the cascade 8 in the filter layer 3 are in this embodiment screwed together.
The cover 1 comprises a cover plate 9 and a boss 10 which are integrally formed and coaxially arranged, the cover plate 9 is a circular thin plate, the boss 10 is cylindrical, and an external thread is formed on the outer side surface of the boss 10 and is used for being connected with the filtrate cylinder 2.
Filtrate section of thick bamboo 2 includes that integrated into one piece and coaxial arrangement's filtrating holds chamber 11 and first connector 12, filtrating holds chamber 11 and is hollow cylinder, and the internal diameter that filtrating held chamber 11 equals with the diameter of boss 10, and filtrating holds the inboard of the one end that first connector 12 was kept away from in chamber 11 and offers the internal thread with boss 10 outside external screw thread assorted, and the filtrating in filtrate section of thick bamboo 2 holds chamber 11 and the boss 10 threaded connection in lid 1. The first connecting head 12 is a hollow cylinder, the outer diameter of the first connecting head 12 is smaller than that of the filtrate containing cavity 11, and the outer side of the first connecting head 12 is provided with an external thread for connecting with the filter layer 3.
As shown in fig. 3, the filter layer 3 includes a seal ring 5, an AAO porous membrane 6, a support sheet 7, and a cascade member 8, which are closely attached in this order. The cascade piece 8 includes connecting seat 13 and the second connector 14 of integrated into one piece and coaxial arrangement, connecting seat 13 is hollow cylinder, and the internal diameter of connecting seat 13 equals with the external diameter of first connector 12, and connecting seat 13 inboard is seted up with first connector 12 outside external screw thread assorted internal thread, and connecting seat 13 of cascade piece 8 and the first connector 12 threaded connection in the filtrating section of thick bamboo 2. Second connector 14 is hollow cylinder, and the external diameter of second connector 14 equals with the internal diameter of connecting seat 13, and the outside of second connector 14 is seted up with the inboard internal thread assorted external screw thread of connecting seat 13, and two filter layers 3 in this embodiment can be through cascade 8 threaded connection.
The sealing ring 5 in the filter layer 3 is annular, the AAO porous membrane 6 and the support sheet 7 are circular, the diameters of the AAO porous membrane 6 and the support sheet 7 are equal to the inner diameter of the connecting seat 13, and the sealing ring 5, the AAO porous membrane 6 and the support sheet 7 are tightly attached to each other and are arranged in the connecting seat 13 of the cascade piece 8. In this example, the pore diameters of the AAO porous membranes 6 in the two filter layers 3 are different, the pore diameter of the AAO porous membrane 6 in the filter layer 3 connected to the lid 1 is 120 nm, the pore diameter of the AAO porous membrane 6 in the filter layer 3 connected to the bottom cylinder 4 is 30 nm, and the pore diameter of the AAO porous membrane in the filter layer 3 located between the two filter layers 3 is 70 nm. The seal ring 5 can prevent the leakage of the filtrate and can restrict the matching of the AAO porous membrane 6, the seal ring 5 and the support sheet 7 to protect the AAO porous membrane 6.
The support sheet 7 in the filter layer 3 needs to support the AAO porous membrane 6 to prevent the AAO porous membrane 6 from being broken under the pressure, and further needs to ensure that the filtrate smoothly passes through, so the middle of the support sheet 7 in this embodiment is provided with a circular through hole array, the boundary of the circular through hole array is arranged in the inner diameter range of the second connector 14, the support sheet 7 is made of a rigid material, the AAO porous membrane 6 can be protected, and the circular through hole array in the middle of the support sheet 7 can be used for discharging the filtrate.
The end section of thick bamboo 4 in this embodiment is one end and seals, and other end open-ended is cylindrical, and the circular cavity has been seted up to end section of thick bamboo 4 is inside, and the internal diameter of end section of thick bamboo 4 equals with the external diameter of second connector 14, and the internal thread that matches with the external screw thread in the second connector 14 outside is seted up to the inboard of end section of thick bamboo 4, and end section of thick bamboo 4 and the second connector 14 threaded connection in the cascade member 8. In this embodiment, the volume of end section of thick bamboo 4 is greater than the volume of filtrate section of thick bamboo 2, can save the washing cost or the replacement cost of centrifuging tube to adding same kind of filtrate in the filtrate section of thick bamboo 2 many times. In this embodiment, the sealing rings 5 of the filtrate cartridge 2 and the filter layer 3 are tightly attached to each other and are in threaded connection with the cascade member 8. The bottom tube 4 and the cascade 8 in the filter layer 3 are in this embodiment screwed together.
Filtrate section of thick bamboo 2 includes that integrated into one piece and coaxial arrangement's filtrating holds chamber 11 and first connector 12, filtrating holds chamber 11 and is hollow cylinder, and the internal diameter that filtrating held chamber 11 equals with the diameter of boss 10, and filtrating holds the inboard of the one end that first connector 12 was kept away from in chamber 11 and offers the internal thread with boss 10 outside external screw thread assorted, and the filtrating in filtrate section of thick bamboo 2 holds chamber 11 and the boss 10 threaded connection in lid 1. The first connecting head 12 is a hollow cylinder, the outer diameter of the first connecting head 12 is smaller than that of the filtrate containing cavity 11, and the outer side of the first connecting head 12 is provided with an external thread for connecting with the filter layer 3.
As shown in fig. 3, the filter layer 3 includes a seal ring 5, an AAO porous membrane 6, a support sheet 7, and a cascade member 8, which are closely attached in this order. The cascade piece 8 includes connecting seat 13 and the second connector 14 of integrated into one piece and coaxial arrangement, connecting seat 13 is hollow cylinder, and the internal diameter of connecting seat 13 equals with the external diameter of first connector 12, and connecting seat 13 inboard is seted up with first connector 12 outside external screw thread assorted internal thread, and connecting seat 13 of cascade piece 8 and the first connector 12 threaded connection in the filtrating section of thick bamboo 2. Second connector 14 is hollow cylinder, and the external diameter of second connector 14 equals with the internal diameter of connecting seat 13, and the outside of second connector 14 is seted up with the inboard internal thread assorted external screw thread of connecting seat 13, and two filter layers 3 in this embodiment can be through cascade 8 threaded connection.
The sealing ring 5 in the filter layer 3 is annular, the AAO porous membrane 6 and the support sheet 7 are circular, the diameters of the AAO porous membrane 6 and the support sheet 7 are equal to the inner diameter of the connecting seat 13, and the sealing ring 5, the AAO porous membrane 6 and the support sheet 7 are tightly attached to each other and are arranged in the connecting seat 13 of the cascade piece 8. In this example, the pore diameters of the AAO porous membranes 6 in the two filter layers 3 are different, the pore diameter of the AAO porous membrane 6 in the filter layer 3 connected to the lid 1 is 150 nm, and the pore diameter of the AAO porous membrane 6 in the filter layer 3 connected to the bottom tube 4 is 30 nm. The seal ring 5 can prevent the leakage of the filtrate and can restrict the matching of the AAO porous membrane 6, the seal ring 5 and the support sheet 7 to protect the AAO porous membrane 6.
As shown in fig. 5 to 10, blood cells in blood having a large diameter, generally greater than 1000 nm, are intercepted by the filter layer 3 near the cap 1 and cannot pass through the AAO porous membrane 6 having a pore diameter of 150 nm. As shown in fig. 11 to 14, the diameter of free particles such as liposome, protein, DNA, etc. in blood is small, the diameter of liposome in blood is generally less than 30 nm, the diameter of hemoglobin is about 25 nm, the diameter of DNA is 2 nm, these particles with fine particle size will pass through AAO porous membrane 6 with pore size of 30 nm, and the diameter of exosome in blood is about 30 nm to 150 nm, and will be intercepted and collected by filter layer 3 near bottom tube 4, this embodiment can obtain exosome in blood by one-time centrifugal filtration.
The support sheet 7 in the filter layer 3 needs to support the AAO porous membrane 6 to prevent the AAO porous membrane 6 from being broken under the pressure, and further needs to ensure that the filtrate smoothly passes through, so the middle of the support sheet 7 in this embodiment is provided with a circular through hole array, the boundary of the circular through hole array is arranged in the inner diameter range of the second connector 14, the support sheet 7 is made of a rigid material, the AAO porous membrane 6 can be protected, and the circular through hole array in the middle of the support sheet 7 can be used for discharging the filtrate.
As shown in fig. 4, in this embodiment, the through hole inside the filtrate cylinder 2 is a diameter-variable through hole, the inner diameter of the filtrate cylinder 2 near one end of the cover 1 is the largest, the inner diameter of the filtrate cylinder 2 near one end of the filter layer 3 is the smallest, and the inner diameter of the filtrate cylinder 2 near one end of the filter layer 3 is smaller than the inner diameter of the seal ring 5; one side of the axial section of the reducing through hole, which is close to the cover 1, is rectangular, one side of the axial section of the reducing through hole, which is close to the filter layer 3, is inverted trapezoidal, and the inner diameter of the reducing through hole is uniformly reduced. The internal diameter of reducing through-hole reduces in the filtrating section of thick bamboo to one end that is close to the filter layer at a filtrating section of thick bamboo reaches the minimum, is favorable to the filtrate to get into the filter layer smoothly, prevents between filtrate infiltration filtrating section of thick bamboo and the filter layer, and simultaneously, the internal diameter uniform variation of reducing through-hole in the filtrating section of thick bamboo can prevent that the filtrate from being detained in the reducing department of reducing through-hole, influences the filter effect.
The end section of thick bamboo 4 in this embodiment is one end and seals, and other end open-ended is cylindrical, and the circular cavity has been seted up to end section of thick bamboo 4 is inside, and the internal diameter of end section of thick bamboo 4 equals with the external diameter of second connector 14, and the internal thread that matches with the external screw thread in the second connector 14 outside is seted up to the inboard of end section of thick bamboo 4, and end section of thick bamboo 4 and the second connector 14 threaded connection in the cascade member 8. In this embodiment, the volume of end section of thick bamboo 4 is greater than the volume of filtrating section of thick bamboo 2, can add twice the same kind blood sample to filtrating section of thick bamboo 2, and needn't change the centrifuging tube, has practiced thrift the cost. In this embodiment, the sealing rings 5 of the filtrate cartridge 2 and the filter layer 3 are tightly attached to each other and are in threaded connection with the cascade member 8. The bottom tube 4 and the cascade 8 in the filter layer 3 are in this embodiment screwed together.
The utility model discloses a theory of operation: compared with the ultrafiltration membrane in the traditional ultrafiltration centrifugal tube, the AAO porous membrane 6 has the advantage of uniform pore diameter, can accurately and effectively control the filtering effect, and improves the accuracy of the ultrafiltration centrifugal tube. However, the AAO porous membrane 6 is a brittle material and may be broken and broken during high-speed centrifugation, resulting in failure of filtration. The utility model discloses increased sealing washer 5 and backing sheet 7 and protected AAO porous membrane 6, backing sheet 7 sets up the reverse side in 6 pressurized one sides of AAO porous membrane, and backing sheet 7 that forms through the rigid material preparation can provide effective support to circular through-hole array has been seted up to the centre of backing sheet 7, in protection AAO porous membrane 6, can make the filtrate pass through smoothly.
In the centrifugal technology, one kind of particles with the particle size in the middle range or different particles with different particle sizes are required to be obtained respectively, for example, a solution containing four kinds of particles with the ABCD particle size is reduced gradually, if the aim of separating the four kinds of particles can be achieved by at least three times of centrifugal filtration by depending on the traditional ultrafiltration centrifugal tube, the three filter layers 3 can be arranged, the middle value of the four kinds of particle sizes is selected by the aperture of the AAO porous membrane 6 in each filter layer 3, the aim of separating the four kinds of particles can be achieved by one-time centrifugal filtration, the A particles are intercepted above the filter layer 3 on the uppermost layer, the B particles are positioned between the filter layer 3 on the uppermost layer and the second filter layer 3, the C particles are positioned between the second filter layer 3 and the third filter layer 3, and the D particles are collected in the bottom barrel 4, the operation process is simplified, the error caused by multiple times of centrifugation is avoided.
Claims (10)
1. Use scalable ultrafiltration centrifuging tube of AAO porous membrane, its characterized in that: the filter comprises a cover (1), a filtrate cylinder (2), a plurality of filter layers (3) and a bottom cylinder (4) which are connected in sequence, wherein the filter layers (3) comprise sealing rings (5), AAO porous membranes (6), supporting sheets (7) and cascade pieces (8) which are tightly attached in sequence, and the filtrate cylinder (2) is tightly attached to the sealing rings (5) in the adjacent filter layers (3); the cascade piece (8) of the filter layer (3) is tightly attached to the sealing ring (5) in the adjacent filter layer (3); the bottom cylinder (4) is connected with a cascade part (8) in the filtering layer (3) adjacent to the bottom cylinder.
2. The expandable ultrafiltration centrifuge tube using an AAO porous membrane of claim 1, wherein: the cover (1) comprises a cover plate (9) and a boss (10) which are integrally formed and coaxially arranged, the cover plate (9) is a circular thin plate, the boss (10) is cylindrical, and an external thread is formed on the outer side surface of the boss (10).
3. The expandable ultrafiltration centrifuge tube using an AAO porous membrane of claim 2, wherein: the filtrate cylinder (2) comprises a filtrate containing cavity (11) and a first connecting head (12) which are integrally formed and coaxially arranged, the filtrate containing cavity (11) is a hollow cylinder, the inner diameter of the filtrate containing cavity (11) is equal to the diameter of the boss (10), and the inner side of one end, away from the first connecting head (12), of the filtrate containing cavity (11) is provided with an internal thread matched with the external thread on the outer side surface of the boss (10); the first connecting head (12) is a hollow cylinder, the outer diameter of the first connecting head (12) is smaller than that of the filtrate containing cavity (11), and an external thread is formed in the outer side of the first connecting head (12).
4. The expandable ultrafiltration centrifuge tube using an AAO porous membrane of claim 3, wherein: the cascade part (8) comprises a connecting seat (13) and a second connecting head (14) which are integrally formed and coaxially arranged, the connecting seat (13) is a hollow cylinder, the inner diameter of the connecting seat (13) is equal to the outer diameter of the first connecting head (12), and the inner side of the connecting seat (13) is provided with an internal thread matched with the external thread on the outer side of the first connecting head (12); the second connector (14) is a hollow cylinder, the outer diameter of the second connector (14) is equal to the inner diameter of the connecting seat (13), and the outer side of the second connector (14) is provided with an outer thread matched with the inner side inner thread of the connecting seat (13).
5. The expandable ultrafiltration centrifuge tube using an AAO porous membrane of claim 4, wherein: the bottom cylinder (4) is in a cylindrical shape with one end closed and the other end opened; a round cavity is formed in the bottom barrel (4), the inner diameter of the bottom barrel (4) is equal to the outer diameter of the second connector (14), and an internal thread matched with an external thread on the outer side of the second connector (14) is formed in the inner side of the bottom barrel (4).
6. The expandable ultrafiltration centrifuge tube using an AAO porous membrane of claim 4, wherein: the sealing ring (5) is annular, the AAO porous membrane (6) and the support sheet (7) are circular, and the diameters of the AAO porous membrane (6) and the support sheet (7) are equal to the inner diameter of the connecting seat (13).
7. The expandable ultrafiltration centrifuge tube using an AAO porous membrane of claim 6, wherein: the through hole in the filtrate cylinder (2) is a reducing through hole, the inner diameter of one end, close to the cover (1), of the filtrate cylinder (2) is the largest, the inner diameter of one end, close to the filter layer (3), of the filtrate cylinder (2) is the smallest, and the inner diameter of one end, close to the filter layer (3), of the filtrate cylinder (2) is smaller than that of the seal ring (5); one side of the axial section of the reducing through hole, which is close to the cover (1), is rectangular, one side of the axial section of the reducing through hole, which is close to the filter layer (3), is inverted trapezoidal, and the inner diameter of the reducing through hole is uniformly reduced.
8. The expandable ultrafiltration centrifuge tube using an AAO porous membrane of claim 5, wherein: the volume of the cavity in the bottom cylinder (4) is larger than that of the cavity in the filtrate cylinder (2).
9. The expandable ultrafiltration centrifuge tube using an AAO porous membrane of claim 6, wherein: the middle of the support sheet (7) is provided with a circular through hole array, and the opening boundary of the circular through hole array is within the inner diameter range of the second connector (14).
10. The expandable ultrafiltration centrifuge tube using an AAO porous membrane of claim 1, wherein: the pore diameter of the AAO porous membrane (6) in the filter layer (3) close to the cover (1) is larger than that of the AAO porous membrane (6) in the filter layer (3) close to the bottom cylinder (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921145962.0U CN210646468U (en) | 2019-07-22 | 2019-07-22 | Expandable ultrafiltration centrifuge tube using AAO porous membrane |
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