CN219409719U

CN219409719U - Natural separation and filtration device for micromonospora criminalis

Info

Publication number: CN219409719U
Application number: CN202320673767.5U
Authority: CN
Inventors: 时圣凤; 叶晖; 毛宁; 刘洪鹏; 孟晓妍; 林雪峰; 曲晏永; 张文浩
Original assignee: Fu'an Pharmaceutical Group Yantai Justawore Pharmaceutical Co ltd
Current assignee: Fu'an Pharmaceutical Group Yantai Justawore Pharmaceutical Co ltd
Priority date: 2023-03-30
Filing date: 2023-03-30
Publication date: 2023-07-25
Anticipated expiration: 2033-03-30

Abstract

The utility model discloses a natural separation and filtration device for micromonospora criminalis, which comprises a culture dish cover, a funnel, a filter paper body, a centrifuge tube and a sterilization bag, wherein the culture dish cover is covered on the upper opening of the funnel, and the filter paper body is formed by overlapping a plurality of layers of mirror wiping paper; the filter paper body is placed on the inner side of the funnel and is attached to the inner wall of the funnel; the funnel is inserted into the centrifuge tube, and the lower part of the funnel is coated with a gauze layer; the gauze layer is movably embedded into the upper part of the inner side of the centrifuge tube; the assembled culture dish cover, the funnel and the centrifuge tube are coated with double-layer gauze, and are fastened by tying ropes or rubber bands, and the lower part of the centrifuge tube protrudes out of the double-layer gauze; the outer part of the double-layer gauze is sleeved with two layers of sterilizing bags. The device for naturally separating and filtering the micromonospora criminalis can filter large-particle impurities such as a culture medium, is not easy to block by multi-layer mirror wiping paper filtration, and can be repeatedly used.

Description

Natural separation and filtration device for micromonospora criminalis

Technical Field

The utility model particularly relates to a natural separation and filtration device for micromonospora criminalis, and belongs to the technical field of micromonospora criminalis separation.

Background

In a laboratory, the strain is often required to be naturally separated, a proper filtering membrane is difficult to purchase, the pore diameter of the filtering membrane is too small, and the filtering membrane is particularly easy to block, so that the test cannot be performed; if the pore diameter of the membrane is too large, impurities cannot be effectively removed, which is disadvantageous to the later operation; for example, the separation and filtration of the micromonospora criminalis are carried out, and the micromonospora criminalis needs to be separated in the culture solution, and the hypha diameter of the micromonospora criminalis is 0.4-0.6 um; the spore diameter is 0.8-1.2 um, the natural separation difficulty of the micromonospora criminalis is relatively high, the existing filtering device is complex in the separation operation process of the strain, and the consumable is relatively high.

Disclosure of Invention

In order to solve the problems, the utility model provides a natural separation and filtration device for micromonospora criminalis, which is simple to operate, can be repeatedly used, reduces consumable materials and saves cost.

The utility model relates to a device for naturally separating and filtering micromonospora criminalis, a culture dish cover,

a funnel, the culture dish cover is covered on the upper opening of the funnel,

the filter paper body is formed by overlapping a plurality of layers of mirror wiping paper; the filter paper body is placed on the inner side of the funnel and is attached to the inner wall of the funnel;

the funnel is inserted into the centrifuge tube, and the lower part of the funnel is coated with a gauze layer; the gauze layer is movably embedded into the upper part of the inner side of the centrifuge tube;

the assembled culture dish cover, the funnel and the centrifuge tube are coated with double-layer gauze, and are fastened by tying ropes or rubber bands, and the lower part of the centrifuge tube protrudes out of the double-layer gauze;

and the outer part of the double-layer gauze is sleeved with two layers of sterilization bags.

Further, filter paper attached to the top surface of the funnel is arranged on the inner side of the culture dish cover, the upper opening of the funnel is covered by a layer of filter paper, the culture dish cover and the funnel opening are convenient to be matched better, and meanwhile operation sundry fungus pollution can be prevented.

Further, the inner diameter of the culture dish cover is 80mm, the diameter of the upper opening of the funnel is 75mm, the diameter of the filter paper is 78mm, and the diameter of the filter paper body is 60mm; the filter paper body adopts four layers of mirror wiping paper, the mirror wiping paper is sheared into pieces with the diameter of 60mm, folded in half, folded into a conical cavity, embedded into the inner side of the funnel and tightly attached to the lower opening of the inner side of the funnel.

Further, the outer edge of the filter paper body is of a lace or edge structure with equal size; the filter paper body is better attached to the inner wall of the funnel through the lace or the edge.

Compared with the prior art, the natural separation and filtration device for the micromonospora criminalis can be used for filtering large-particle impurities such as a culture medium in the natural separation process of the micromonospora criminalis, is not easy to block by multi-layer mirror wiping paper filtration, has a simple structure, is convenient to operate, can be repeatedly used, reduces consumable materials, saves economic cost, and can also be used for filtering strains with characteristics similar to the micromonospora criminalis, and the application range is wide.

Drawings

Fig. 1 is a schematic diagram of the overall structure of the present utility model.

FIG. 2 is a schematic view of the structure of the culture dish cover of the utility model.

FIG. 3 is a schematic diagram of the structure of a filter according to the present utility model.

Detailed Description

Examples

The micromonospora magenta natural separation filter device as shown in figures 1 and 2,

the culture dish cover 1 is provided with a plurality of culture dish holes,

a funnel 2, the culture dish cover 1 is covered on the upper opening of the funnel 2,

the filter paper body 3 is formed by overlapping a plurality of layers of mirror wiping paper; the filter paper body 3 is placed on the inner side of the funnel 2 and is attached to the inner wall of the funnel 2;

the funnel 2 is inserted into the centrifuge tube 4, and a gauze layer 5 is coated on the lower part of the funnel 2; the gauze layer 5 is movably embedded into the upper part of the inner side of the centrifuge tube 4;

the assembled culture dish cover 1, the funnel 2 and the centrifuge tube 4 are coated with double-layer gauze 6, and are fastened by tying ropes 7 or rubber bands, and the lower part of the centrifuge tube 4 protrudes out of the double-layer gauze 6;

and a sterilizing bag 8, wherein two layers of sterilizing bags 8 are sleeved outside the double-layer gauze 6.

The inboard filter paper 9 that is provided with the laminating of funnel top surface of culture dish lid 1, the funnel upper mouth covers with one deck filter paper, and the culture dish lid of being convenient for better coincide with the funnel mouth, can prevent simultaneously that the operation from miscellaneous fungus from polluting.

The inner diameter of the culture dish cover 1 is 80mm, the diameter of the upper opening of the funnel 2 is 75mm, the diameter of the filter paper 9 is 78mm, and the diameter of the filter paper body 3 is 60mm; the filter paper body 3 adopts four layers of mirror wiping paper, the mirror wiping paper is sheared into pieces with the diameter of 60mm, folded in half, folded into a conical cavity, embedded into the inner side of the funnel 2 and tightly attached to the lower opening of the inner side of the funnel 2; the selection of the centrifuge tube 4 is determined according to the diameter and the length of the lower port of the funnel, the diameter is 15mm, the length is 100mm, and a commonly used 10ml centrifuge tube in a laboratory just meets the requirements; proper amount of gauze is wound on the funnel neck, so that the funnel neck and the funnel neck are convenient to fix.

The outer edge of the filter paper body 3 is of a lace or edge structure with equal size; the filter paper body is better attached to the inner wall of the funnel through the lace or the edge.

The utility model relates to a natural separation and filtration device for micromonospora criminalis, which comprises the following assembly method: cutting a piece of round filter paper with the diameter of 78mm, paving the round filter paper in a culture dish cover with the diameter of 80mm, cutting four layers of mirror wiping paper with the diameter of 60mm, folding the round filter paper in half, folding the round filter paper into a cone, putting the four layers of mirror wiping paper into a funnel with the diameter of 75mm, reversely buckling the funnel into the culture dish cover paved with the filter paper, winding a plurality of circles of gauze at the joint of the upper opening and the lower opening of the funnel, embedding the funnel into a centrifuge tube, wrapping the culture dish cover, the funnel and the centrifuge tube which are fixedly assembled by using double-layer gauze, and putting the device into a sterilizing bag, and putting two layers of sterilizing bags into the sterilizing bag for sterilization.

The utility model relates to a natural separation and filtration device for micromonospora criminalis, which comprises the following steps: before the test starts, one layer of sterilization bag of the device is taken down, the device is placed on an ultra-clean workbench, self-cleaning is carried out for 5min, a second layer of sterilization bag is taken down, a centrifuge tube rack is placed at the lower part of a centrifuge tube of the device, when the device is used, double-layer gauze is taken down, a culture dish cover is gently taken down, filter paper above a funnel opening is gently taken down by using sterilized tweezers, bacterial liquid is poured into a filter funnel of four layers of lens wiping paper, the filter paper is gently covered by using the sterilized tweezers, the contamination of bacteria is prevented, the filtration is finished, filter paper at the upper opening of the funnel is taken down, the upper opening of the funnel is held by one hand, gauze at the centrifuge tube opening is clamped by the other hand, the funnel is taken down, a centrifuge tube cover is covered, the bacterial liquid is ready for use, and the filtration operation is finished.

The above embodiments are merely preferred embodiments of the present utility model, and all changes and modifications that come within the meaning and range of equivalency of the structures, features and principles of the utility model are therefore intended to be embraced therein.

Claims

1. A kind of red micromonospora natural separation filter equipment, characterized in that:

a cover of the culture dish,

a funnel, the culture dish cover is covered on the upper opening of the funnel,

2. The device for naturally separating and filtering micromonospora criminalis according to claim 1, characterized in that: the inboard of culture dish lid is provided with the filter paper of laminating with the funnel top surface.

3. The device for naturally separating and filtering micromonospora criminalis according to claim 1, characterized in that: the inner diameter of the culture dish cover is 80mm, the diameter of the upper opening of the funnel is 75mm, the diameter of the filter paper is 78mm, and the diameter of the filter paper body is 60mm.

4. The device for naturally separating and filtering micromonospora criminalis according to claim 1, characterized in that: the outer edge of the filter paper body is of a lace or edge structure with equal size.