JP3157973U - Optical microscope illumination device - Google Patents

Optical microscope illumination device Download PDF

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JP3157973U
JP3157973U JP2009006538U JP2009006538U JP3157973U JP 3157973 U JP3157973 U JP 3157973U JP 2009006538 U JP2009006538 U JP 2009006538U JP 2009006538 U JP2009006538 U JP 2009006538U JP 3157973 U JP3157973 U JP 3157973U
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observation
illumination
observation sample
optical microscope
objective lens
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久幸 高島
久幸 高島
朋美 高島
朋美 高島
優花 高島
優花 高島
彩 高島
彩 高島
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久幸 高島
久幸 高島
朋美 高島
朋美 高島
優花 高島
優花 高島
彩 高島
彩 高島
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Abstract

【課題】光学顕微鏡において、安価でかつ、もともと暗視野観察用照明あるいは実体観察用照明のための構成をもたない光学顕微鏡に後付けが可能な照明装置で、観察試料の近傍に移動可能な状態で配置した簡易な構成により明視野照明および暗視野照明が実施できるとともに、明視野光学系と暗視野光学系のいずれにも切り換えても照明が得られ、しかも軽量、小型化および省電力化が実現できる透過型顕微鏡および実体顕微鏡の照明装置を提供する。【解決手段】本照明装置を、もともと暗視野照明あるいは実体観察用照明のための構成をもたない既存の透過型光学顕微鏡および実体顕微鏡に装着することにより、明視野系透過および暗視野系透過観察、並びに実体観察が可能となり、必要に応じ3次元的に配置された複数個の光源を選択的に点灯させる。また、光源の色種を替えることにより、フィルターを交換して観察する事と同程度の効果となる。【選択図】図1In an optical microscope, an illumination device that can be retrofitted to an optical microscope that is inexpensive and does not originally have a configuration for dark field observation illumination or entity observation illumination, and is movable in the vicinity of an observation sample With the simple configuration arranged in, bright-field illumination and dark-field illumination can be implemented, and illumination can be obtained by switching to either the bright-field optical system or the dark-field optical system, and it is lightweight, compact, and power-saving Provided is an illumination device for a transmission microscope and a stereomicroscope that can be realized. The illumination apparatus is mounted on an existing transmission optical microscope and a stereoscopic microscope that are not originally configured for dark field illumination or stereoscopic observation illumination, thereby transmitting bright field transmission and dark field transmission. Observation and entity observation are possible, and a plurality of light sources arranged three-dimensionally are selectively turned on as necessary. In addition, changing the color type of the light source has the same effect as observing with the filter replaced. [Selection] Figure 1

Description

本考案は、光学顕微鏡に用いられる明視野照明および暗視野照明、並びに実体観察用照明に関するものである。   The present invention relates to bright-field illumination and dark-field illumination used for an optical microscope, and entity observation illumination.

従来、光学顕微鏡には、明視野では見えづらい観察試料(標本)の傷等を観察するために暗視野観察を行えるようにした暗視野顕微鏡がある。このような暗視野観察が行える光学顕微鏡は、観察試料(標本)を照明するための暗視野照明装置を有している。   2. Description of the Related Art Conventionally, optical microscopes include dark field microscopes that can perform dark field observation in order to observe scratches or the like on an observation sample (specimen) that is difficult to see in a bright field. An optical microscope capable of performing such dark field observation has a dark field illumination device for illuminating an observation sample (specimen).

従来、暗視野照明装置として、例えば、特許文献1、2および3に示されるように、照明光源から観察試料(標本)に至る途中の光路にリング状のファイバを挿入し、このファイバによるリング照明により暗視野照明を行う装置が多く提案されている。   Conventionally, as a dark field illumination device, for example, as shown in Patent Documents 1, 2, and 3, a ring-shaped fiber is inserted into an optical path from an illumination light source to an observation sample (specimen), and ring illumination using this fiber is performed. Many devices for performing dark field illumination have been proposed.

特許文献1の暗視野照明装置は、対物レンズを囲む暗視野照明用光路と、前記暗視野照明光路によって導かれた照明光で観察試料(標本)を照明する光学顕微鏡の落射照明装置である。この特許文献1の暗視野照明装置は、暗視野照明に照明光を供給する環状光源をレボルバ内に設けている。   The dark field illumination device of Patent Document 1 is an epi-illumination device for an optical microscope that illuminates an observation sample (specimen) with an illumination light path surrounding an objective lens and illumination light guided by the dark field illumination light path. In the dark field illumination device of Patent Document 1, an annular light source that supplies illumination light to dark field illumination is provided in a revolver.

特許文献2の暗視野照明装置は、落射照明光を導入するハーフミラーと、対物レンズの間に環状ファイバの出射端とを有し、落射照明系中の光源と共役な位置にファイバの入射端または開口絞りを切り換え可能に配置している。   The dark field illumination device of Patent Document 2 has a half mirror for introducing epi-illumination light and an exit end of an annular fiber between objective lenses, and an incident end of the fiber at a position conjugate with the light source in the epi-illumination system. Or the aperture stop is arranged to be switchable.

特許文献3の暗視野照明装置は、観察光路中に選択的に配置される対物レンズを備えた光学顕微鏡に設けられている。この特許文献3の暗視野照明装置は、対物レンズに脱着可能に設けられ光入射部と光入射部からの光を対象物へ照射する光照射部とを有する光伝達手段を備えた照明手段と、照明手段とは機械的に分離され照明手段の光入射部に向けて光りを照射する光源と、を有している。   The dark field illumination device of Patent Document 3 is provided in an optical microscope including an objective lens that is selectively disposed in an observation optical path. The dark field illumination device of Patent Document 3 is provided with a light transmission means that is detachably attached to an objective lens and includes a light incident part and a light irradiation part that irradiates an object with light from the light incident part; The light source has a light source that is mechanically separated from the light unit and emits light toward the light incident portion of the light unit.

特許第3080393号公報「落射照明装置」Japanese Patent No. 3080393 “Epi-illumination device” 特開2003−5082号公報「顕微鏡システム」Japanese Patent Laid-Open No. 2003-5082 “Microscope System” 特許第3321260号公報「外観検査装置」Japanese Patent No. 3322260 “Appearance Inspection Device”

特許文献1の暗視野照明装置は、暗視野照明するために、対物レンズを囲む暗視野照明用光路と、光学顕微鏡本体に対して脱着可能なレボルバとが必要である。暗視野照明用光路を有する対物レンズは、一般的な対物レンズとは取り付けネジ径が異なる。具体的には、暗視野照明用光路を有する対物レンズは、暗視野照明光路を持たない対物レンズに比べて、ネジ径が大きい。このように、暗視野照明光路を持つ対物レンズは、特殊な形状であるため、製造コストが高くなってしまう。また、脱着可能なレボルバは、レボルバ自体が高価であるとともに、レボルバが脱着できるように光学顕微鏡本体を構成する事により、光学顕微鏡本体自体の製造コストも上昇してしまう。   The dark field illumination device of Patent Document 1 requires a dark field illumination optical path that surrounds the objective lens and a revolver that can be attached to and detached from the optical microscope body in order to perform dark field illumination. An objective lens having an optical path for dark field illumination has a different mounting screw diameter from a general objective lens. Specifically, an objective lens having a dark field illumination optical path has a larger screw diameter than an objective lens not having a dark field illumination optical path. Thus, since the objective lens having the dark field illumination optical path has a special shape, the manufacturing cost is increased. Moreover, the detachable revolver is expensive in itself, and the manufacturing cost of the optical microscope body itself is increased by configuring the optical microscope body so that the revolver can be detached.

このように、特許文献1の暗視野照明装置は、通常の安価な光学顕微鏡には適用し難いという問題がある。   Thus, the dark field illumination device of Patent Document 1 has a problem that it is difficult to apply to a normal inexpensive optical microscope.

また、特許文献2の暗視野照明装置は、特許文献1と同様に暗視野照明光路を有する対物レンズを採用しているため、対物レンズの価格が高くなってしまう。また、落射照明系中の光源と共役な位置に、ファイバの入射端または開口絞りを切り換え可能にするためにはかなりのスペースが必要であり、顕微鏡本体内部に落射照明装置が内蔵される事が多い倒立型の光学顕微鏡においては、顕微鏡本体そのものが大型化してしまうという欠点がある。また、切り換え機構等を配置する事により、大幅な製造コストの上昇が予想される。このため、特許文献2の暗視野照明装置も、やはり通常の安価な光学顕微鏡には適用し難いという問題を有する。   Moreover, since the dark field illumination device of Patent Document 2 employs an objective lens having a dark field illumination optical path as in Patent Document 1, the price of the objective lens is increased. In addition, a considerable space is required to switch the incident end of the fiber or the aperture stop at a position conjugate with the light source in the epi-illumination system, and the epi-illumination device may be built in the microscope body. In many inverted optical microscopes, there is a drawback that the microscope body itself becomes large. In addition, a significant increase in manufacturing cost is expected by arranging a switching mechanism and the like. For this reason, the dark field illumination device of Patent Document 2 also has a problem that it is difficult to apply to a normal inexpensive optical microscope.

また、特許文献3の暗視野照明装置は、対物レンズを囲む暗視野照明用光路と顕微鏡本体に対して脱着可能なレボルバ等を必要としないため、対物レンズと顕微鏡本体の製造コストの上昇という問題は回避される。しかしながら、特許文献3の暗視野照明装置における照明手段は、光入射部と光出射部との間をファイバ等により光を伝達する構成であるため、この構成による照明手段の製造コストも高価なものとなってしまう。さらに、前記照明手段は、暗視野観察を実施したい対物レンズの本数だけ用意する必要がある。このため、特許文献3の暗視野照明装置を用いた光学顕微鏡システムは、システム全体としての製造コストが高くなるという欠点がある。   Further, the dark field illumination device of Patent Document 3 does not require a dark field illumination optical path that surrounds the objective lens and a revolver that can be attached to and detached from the microscope main body, which increases the manufacturing cost of the objective lens and the microscope main body. Is avoided. However, since the illumination means in the dark field illumination device of Patent Document 3 is configured to transmit light between the light incident portion and the light emission portion by a fiber or the like, the manufacturing cost of the illumination means with this configuration is also expensive. End up. Further, it is necessary to prepare as many illumination means as the number of objective lenses for which dark field observation is desired. For this reason, the optical microscope system using the dark field illumination device of Patent Document 3 has a drawback that the manufacturing cost of the entire system increases.

上記課題を鑑みて、本発明の目的は、光学顕微鏡の観察において、安価でかつ、もともと暗視野照明のための構成をもたない光学顕微鏡に後付けが可能な照明装置で、さらに観察試料(標本)の近傍に配置した簡易な構成により、安価で明視野照明および暗視野照明を行えるとともに、明視野光学系と暗視野光学系のいずれにも切り換えても照明が得られ、しかも軽量、小型化および省電力化が実現できる光学顕微鏡の照明装置を提供する。   In view of the above problems, an object of the present invention is to provide an illumination device that is inexpensive and can be retrofitted to an optical microscope that does not originally have a configuration for dark field illumination. ) Can be used at low cost for bright-field illumination and dark-field illumination, and can be switched to either bright-field optical system or dark-field optical system, and is lightweight and compact. An illumination device for an optical microscope capable of realizing power saving is provided.

本考案の光学顕微鏡の照明装置は、上記課題を鑑みて、以下の構成を有している。   The illumination device for an optical microscope of the present invention has the following configuration in view of the above problems.

本考案の一態様の顕微鏡用照明装置は、対物レンズと、観察時に観察試料が配置される着脱可能な観察試料支持部(ステージ)とを有する光学顕微鏡に用いられる。この照明装置は、電源(例えば、乾電池)、スイッチおよび照明光を出力する光出射部を有し、光出射部は前記観察試料支持部(ステージ)の観察試料の近傍に配置される。暗視野系観察として使用する場合には、照明装置は対物レンズの光軸において、前記対物レンズに対して観察試料支持部(ステージ)を挟んで反対側に配置される。この照明装置は、前記照明光を前記観察試料(標本)に向けて、前記観察試料支持部(ステージ)の斜め下方から照射すると、前記観察試料(標本)からの反射光は、前記対物レンズの光軸に入射するように配置されている。観察に必要に応じ、3次元的に配置された複数個の光源(例えば、超高輝度発光ダイオード)を選択的に点灯させる。   The illumination device for a microscope according to one embodiment of the present invention is used for an optical microscope having an objective lens and a removable observation sample support (stage) on which an observation sample is arranged at the time of observation. This illuminating device has a power source (for example, a dry cell), a switch, and a light emitting part that outputs illumination light, and the light emitting part is disposed in the vicinity of the observation sample on the observation sample support part (stage). When used for dark field system observation, the illuminating device is disposed on the opposite side of the objective lens with the observation sample support (stage) in between on the optical axis of the objective lens. When this illumination device irradiates the illumination light toward the observation sample (specimen) from obliquely below the observation sample support (stage), reflected light from the observation sample (specimen) is reflected by the objective lens. It arrange | positions so that it may inject into an optical axis. As necessary for observation, a plurality of light sources (for example, ultra-bright light emitting diodes) arranged three-dimensionally are selectively turned on.

本考案の他の様の顕微鏡用照明装置は、対物レンズと、観察時に観察試料が配置される着脱可能な観察試料支持部(ステージ)とを有する光学顕微鏡に用いられる。この照明装置は、電源(例えば、乾電池)、スイッチおよび照明光を出力する光出射部を有し、光出射部は前記観察試料支持部(ステージ)の観察試料の近傍に配置される。明視野系観察として使用する場合には、照明装置は対物レンズの光軸において、前記対物レンズに対して観察試料支持部(ステージ)を挟んで反対側に配置される。この照明装置は、前記照明光を前記観察試料に向けて、下方から照射すると、前記観察試料(標本)からの透過光は、前記対物レンズの光軸に入射するように配置されている。観察に必要に応じ3次元的に配置された複数個の光源(例えば、超高輝度発光ダイオード)を選択的に点灯させる。   The microscope illumination device according to another aspect of the present invention is used in an optical microscope having an objective lens and a removable observation sample support (stage) on which an observation sample is arranged during observation. This illuminating device has a power source (for example, a dry cell), a switch, and a light emitting part that outputs illumination light, and the light emitting part is disposed in the vicinity of the observation sample on the observation sample support part (stage). When used for bright field system observation, the illuminating device is arranged on the opposite side of the objective lens with the observation sample support (stage) in between on the optical axis of the objective lens. This illuminating device is arranged so that transmitted light from the observation sample (specimen) is incident on the optical axis of the objective lens when the illumination light is directed toward the observation sample from below. A plurality of light sources (for example, ultra-bright light emitting diodes) arranged three-dimensionally as needed for observation are selectively turned on.

本考案のさらに他の様の顕微鏡用照明装置は、対物レンズと、観察時に観察試料が配置される着脱可能な観察試料支持部(ステージ)とを有する光学顕微鏡に用いられる。この照明装置は、電源(例えば、乾電池)、スイッチおよび照明光を出力する光出射部を有し、光出射部は前記観察試料支持部(ステージ)の観察試料の近傍に配置される。通常の透過型光学顕微鏡を実体顕微鏡として使用する場合には、観察試料支持部に対して対物レンズと同じ側に配置される。この照明装置は、前記照明光を前記観察試料に向けて、斜め上方から照射すると、前記観察試料(標本)からの反射光は、前記対物レンズの光軸に入射するように配置されている。観察に必要に応じ3次元的に配置された複数個の光源(例えば、超高輝度発光ダイオード)を選択的に点灯させる。   The illumination device for a microscope according to still another aspect of the present invention is used in an optical microscope having an objective lens and a removable observation sample support (stage) on which an observation sample is arranged at the time of observation. This illuminating device has a power source (for example, a dry cell), a switch, and a light emitting part that outputs illumination light, and the light emitting part is disposed in the vicinity of the observation sample on the observation sample support part (stage). When a normal transmission optical microscope is used as a stereomicroscope, it is arranged on the same side as the objective lens with respect to the observation sample support. This illuminating device is arranged so that the reflected light from the observation sample (specimen) is incident on the optical axis of the objective lens when the illumination light is directed toward the observation sample from obliquely above. A plurality of light sources (for example, ultra-bright light emitting diodes) arranged three-dimensionally as needed for observation are selectively turned on.

本考案は、光学顕微鏡の観察において、安価でかつ、もともと暗視野照明あるいは実体観察用照明のための構成をもたない透過型光学顕微鏡に後付けが可能な照明装置で、さらに試料の近傍に配置した簡易な構成により明視野照明および暗視野照明を行えるとともに、明視野光学系と暗視野光学系のいずれにも切り換えても照明が得られ、しかも軽量、小型化および省電力化が実現できる顕微鏡の照明装置を提供し得る。   The present invention is an illumination device that can be retrofitted to a transmission type optical microscope that is inexpensive and does not originally have a configuration for dark field illumination or entity observation illumination, and is arranged near the sample. A microscope that can perform bright-field illumination and dark-field illumination with a simple configuration, and can obtain illumination by switching to either a bright-field optical system or a dark-field optical system, and can achieve light weight, miniaturization, and power saving. Can be provided.

図1は、光学顕微鏡の照明装置の側面図である。FIG. 1 is a side view of an illumination device of an optical microscope. 図2は、実施例1の形態に従った暗視野系観察として、透過型光学顕微鏡に照明装置が適用された概略的な側面図である。FIG. 2 is a schematic side view in which an illuminating device is applied to a transmission optical microscope as dark field system observation according to the mode of the first embodiment. 図3は、実施例2の形態に従った明視野系観察として、透過型光学顕微鏡に照明装置が適用された概略的な側面図である。FIG. 3 is a schematic side view in which an illuminating device is applied to a transmission optical microscope as a bright field system observation according to the mode of the second embodiment. 図4は、実施例3の形態に従った実体観察として、透過型光学顕微鏡に照明装置が適用された概略的な側面図である。FIG. 4 is a schematic side view in which an illuminating device is applied to a transmission type optical microscope as entity observation according to the form of Example 3.

以下、本考案の実施の形態を図に基づいて説明する。   Hereinafter, embodiments of the present invention will be described with reference to the drawings.

図1は本考案装置の側面図であって、図2、図3および図4は本考案の実施形態について透過型光学顕微鏡に適用した場合の全体構成図であり、図2は暗視野照明、図3は明視野照明、図4は実体観察用照明をそれぞれ示す。   FIG. 1 is a side view of the device of the present invention, and FIGS. 2, 3 and 4 are overall configuration diagrams when the embodiment of the present invention is applied to a transmission optical microscope. FIG. FIG. 3 shows bright field illumination, and FIG. 4 shows entity observation illumination.

図2は、本考案装置の実施例1の側面図であって、暗視野系観察として使用する場合、透過型光学顕微鏡に照明装置が適用された概略的な構造を示す。   FIG. 2 is a side view of Example 1 of the device of the present invention, and shows a schematic structure in which an illumination device is applied to a transmission optical microscope when used as dark field system observation.

本考案の照明装置は、光学顕微鏡の観察において、安価でかつ、もともと暗視野照明のための構成をもたない光学顕微鏡に後付けが可能な照明装置で、さらに観察試料(標本)の近傍に配置した簡易な構成により暗視野照明を得る事ができ、しかも軽量、小型化および省電力化が実現できる光学顕微鏡の照明装置である。   The illuminating device of the present invention is an illuminating device that is inexpensive and can be retrofitted to an optical microscope that does not originally have a configuration for dark field illumination, and is arranged near the observation sample (specimen). This is an illumination device for an optical microscope that can provide dark field illumination with a simple configuration and that can achieve light weight, downsizing, and power saving.

対物レンズと、観察時に観察試料が配置される着脱可能な観察試料支持部(ステージ)とを有する光学顕微鏡に用いる照明装置であって、照明光を出力する光出射部を有し、前記観察試料支持部の観察試料の近傍に配置される。暗視野系観察として使用する場合には、照明装置は対物レンズの光軸において、前記対物レンズに対して観察試料支持部(ステージ)を挟んで反対側に配置される。前記照明光を前記観察試料に向けて、前記観察試料支持部(ステージ)の斜め下方から照射すると、反射光は対物レンズに入射する。もともと暗視野照明のための構成をもたない既存の光学顕微鏡に後付けする事により、暗視野系観察が容易に可能となる。観察に必要に応じ3次元的に配置された複数個の光源(例えば、超高輝度発光ダイオード)を選択的に点灯させる。また、光源(例えば、超高輝度発光ダイオード)の色種(白色、青色、赤色、電球色等)を替える事により、フィルターを交換して観察する事と同程度の効果となる。   An illumination device used in an optical microscope having an objective lens and a detachable observation sample support (stage) on which an observation sample is arranged at the time of observation, the illumination device including a light emitting unit that outputs illumination light, and the observation sample It arrange | positions in the vicinity of the observation sample of a support part. When used for dark field system observation, the illuminating device is disposed on the opposite side of the objective lens with the observation sample support (stage) in between on the optical axis of the objective lens. When the illumination light is directed toward the observation sample and irradiated from obliquely below the observation sample support portion (stage), the reflected light enters the objective lens. By retrofitting an existing optical microscope that originally has no configuration for dark field illumination, dark field observation can be easily performed. A plurality of light sources (for example, ultra-bright light emitting diodes) arranged three-dimensionally as needed for observation are selectively turned on. Further, by changing the color type (white, blue, red, light bulb color, etc.) of the light source (for example, ultra-high luminance light emitting diode), the effect is the same as that obtained by exchanging the filter.

図3は、本考案装置の実施例2の側面図であって、明視野系観察として使用する場合、
透過型光学顕微鏡に照明装置が適用された概略的な構造を示す。
FIG. 3 is a side view of Example 2 of the device of the present invention, and when used for bright field system observation,
1 shows a schematic structure in which an illumination device is applied to a transmission optical microscope.

本考案は、光学顕微鏡の観察において、安価でかつ、透過照明装置等、もともと明視野照明のための構成をもたない顕微鏡に後付けが可能な照明装置で、さらに観察試料(標本)の近傍に配置した簡易な構成により明視野照明を得る事ができ、しかも軽量、小型化および省電力化が実現できる光学顕微鏡の照明装置である。   The present invention is an illumination device that can be retrofitted to a microscope that does not have a configuration for bright-field illumination, such as a transmission illumination device, for observation with an optical microscope, and in the vicinity of an observation sample (specimen). It is an illumination device for an optical microscope that can obtain bright-field illumination with a simple arrangement and that can achieve light weight, downsizing, and power saving.

対物レンズと、観察時に観察試料が配置される着脱可能な観察試料支持部(ステージ)とを有する光学顕微鏡に用いる照明装置であって、照明光を出力する光出射部を有し、前記観察試料支持部(ステージ)の観察試料の近傍に配置される。明視野系観察として使用する場合には、照明装置は対物レンズの光軸において、前記対物レンズに対して観察試料支持部(ステージ)を挟んで反対側に配置される。前記照明光を前記観察試料に向けて、前記観察試料支持部(ステージ)の下方から照射すると、透過光は対物レンズに入射する。透過照明装置等、もともと明視野照明のための構成をもたない既存の簡易な透過型光学顕微鏡に後付けする事により、明視野系観察が容易に可能となる。観察に必要に応じ3次元的に配置された複数個の光源(例えば、超高輝度発光ダイオード)を選択的に点灯させる。また、光源(例えば、超高輝度発光ダイオード)の色種(白色、青色、赤色、電球色等)を替える事により、フィルターを交換して観察する事と同程度の効果となる。   An illumination device used in an optical microscope having an objective lens and a detachable observation sample support (stage) on which an observation sample is arranged at the time of observation, the illumination device including a light emitting unit that outputs illumination light, and the observation sample It arrange | positions in the vicinity of the observation sample of a support part (stage). When used for bright field system observation, the illuminating device is arranged on the opposite side of the objective lens with the observation sample support (stage) in between on the optical axis of the objective lens. When the illumination light is directed toward the observation sample and irradiated from below the observation sample support (stage), the transmitted light enters the objective lens. By retrofitting an existing simple transmission optical microscope that does not originally have a configuration for bright field illumination such as a transmission illumination device, bright field system observation can be easily performed. A plurality of light sources (for example, ultra-bright light emitting diodes) arranged three-dimensionally as needed for observation are selectively turned on. Further, by changing the color type (white, blue, red, light bulb color, etc.) of the light source (for example, ultra-high luminance light emitting diode), the effect is the same as that obtained by exchanging the filter.

図4は、本考案装置の実施例3の側面図であって、実体観察として使用する場合、透過型光学顕微鏡に照明装置が適用された概略的な構造を示す。   FIG. 4 is a side view of Embodiment 3 of the device of the present invention, and shows a schematic structure in which an illuminating device is applied to a transmission type optical microscope when used as entity observation.

本考案は、光学顕微鏡の観察において、安価でかつ、もともと実体観察用照明のための構成をもたない顕微鏡に後付けが可能な照明装置で、さらに観察試料(標本)の近傍に配置した簡易な構成により実体観察用照明を得る事ができ、しかも軽量、小型化および省電力化が実現できる光学顕微鏡の照明装置である。   The present invention is an illumination device that is inexpensive and can be retrofitted to a microscope that does not originally have a configuration for observing an optical microscope. Further, the present invention is a simple device arranged near the observation sample (specimen). It is an illumination device for an optical microscope that can obtain illumination for entity observation according to the configuration, and can achieve light weight, downsizing, and power saving.

対物レンズと、観察時に観察試料が配置される着脱可能な観察試料支持部(ステージ)とを有する光学顕微鏡に用いる照明装置であって、照明光を出力する光出射部を有し、前記観察試料支持部(ステージ)の観察試料(標本)の近傍に配置される。通常の透過型光学顕微鏡を実体顕微鏡として使用する場合には、照明装置は観察試料支持部(ステージ)に対して対物レンズと同じ側に配置される。前記照明光を前記観察試料に向けて、斜め上方から照射すると、反射光は対物レンズに入射する。実体顕微鏡用照明の構成をもたない既存の透過型光学顕微鏡に後付けする事により、実体観察が容易に可能になる。観察に必要に応じ3次元的に配置された複数個の光源(例えば、超高輝度発光ダイオード)を選択的に点灯させる。また、光源(例えば、超高輝度発光ダイオード)の色種(白色、青色、赤色、電球色等)を替える事により、フィルターを交換して観察する事と同程度の効果となる。   An illumination device used in an optical microscope having an objective lens and a detachable observation sample support (stage) on which an observation sample is arranged at the time of observation, the illumination device including a light emitting unit that outputs illumination light, and the observation sample It arrange | positions in the vicinity of the observation sample (specimen) of a support part (stage). When a normal transmission optical microscope is used as a stereomicroscope, the illumination device is arranged on the same side as the objective lens with respect to the observation sample support (stage). When the illumination light is directed toward the observation sample from obliquely above, the reflected light enters the objective lens. By retrofitting an existing transmission optical microscope that does not have a stereo microscope illumination configuration, it is possible to easily perform stereo observation. A plurality of light sources (for example, ultra-bright light emitting diodes) arranged three-dimensionally as needed for observation are selectively turned on. Further, by changing the color type (white, blue, red, light bulb color, etc.) of the light source (for example, ultra-high luminance light emitting diode), the effect is the same as that obtained by exchanging the filter.

以上述べたように本考案の照明装置によれば、もともと暗視野照明のための構成をもたない既存の透過型光学顕微鏡および実体顕微鏡に装着する事により、明視野系透過および暗視野系透過観察、並びに実体観察が容易に可能となるとともに、安価、軽量、小型、長寿命および省電力な照明装置を提供できる。   As described above, according to the illumination device of the present invention, bright field transmission and dark field transmission can be achieved by mounting on existing transmission optical microscopes and stereomicroscopes that are not originally configured for dark field illumination. Observation and entity observation can be easily performed, and an inexpensive, lightweight, compact, long-life and power-saving lighting device can be provided.

1 発光部
2 カバー
3 電源(乾電池)
4 メインスイッチ
5 切り換えスイッチ
6 可動型アーム
7 ベース部
8 支持部
9 導線
10 照明光
11 反射光
12 透過光
100 対物レンズの光軸
101 対物レンズ
102 観察試料支持部
103 観察試料
104 レボルバ
105 絞り
1 Light Emitting Unit 2 Cover 3 Power Supply (Battery)
4 Main switch 5 Changeover switch 6 Movable arm 7 Base part 8 Support part 9 Conductor 10 Illumination light 11 Reflected light 12 Transmitted light 100 Optical axis 101 of objective lens Objective lens 102 Observation sample support part 103 Observation sample 104 Revolver 105 Diaphragm

Claims (4)

光学顕微鏡において観察試料を照明するための発光部を有する照明装置で、対物レンズと、観察試料支持部とを有する前記光学顕微鏡に用いる照明装置であって、ベース部、電源、スイッチ、可動型アームおよび発光部を有し、前記発光部は前記観察試料支持部の前記観察試料の近傍に移動可能な状態で配置され、暗視野系観察として使用する場合には、前記発光部は前記対物レンズの光軸において、前記対物レンズに対して前記観察試料支持部を挟んで反対側に配置され、前記発光部からの照明光を前記観察試料に向けて、前記観察試料支持部の斜め下方から照射すると、前記観察試料からの反射光は前記対物レンズの光軸に入射され、暗視野系観察が容易にできる事を特徴とする光学顕微鏡の照明装置。   An illuminating device having a light emitting unit for illuminating an observation sample in an optical microscope, the illuminating device used for the optical microscope having an objective lens and an observation sample supporting unit, comprising a base unit, a power source, a switch, and a movable arm And the light emitting unit is arranged in a movable state in the vicinity of the observation sample of the observation sample support unit, and when used for dark field system observation, the light emitting unit is provided on the objective lens. The optical axis is disposed on the opposite side of the observation sample support portion with respect to the objective lens, and the illumination light from the light emitting portion is directed toward the observation sample from obliquely below the observation sample support portion. An illumination apparatus for an optical microscope characterized in that reflected light from the observation sample is incident on the optical axis of the objective lens, and dark field system observation can be easily performed. 光学顕微鏡において観察試料を照明するための発光部を有する照明装置で、対物レンズと、観察試料支持部とを有する前記光学顕微鏡に用いる照明装置であって、ベース部、電源、スイッチ、可動型アームおよび発光部を有し、前記発光部は前記観察試料支持部の前記観察試料の近傍に移動可能な状態で配置され、明視野系観察として使用する場合には、前記発光部は前記対物レンズの光軸において、前記対物レンズに対して前記観察試料支持部を挟んで反対側に配置され、前記発光部からの照明光を前記観察試料に向けて、前記観察試料支持部の下方から照射すると、前記観察試料からの透過光は前記対物レンズの光軸に入射され、明視野系観察が容易にできる事を特徴とする光学顕微鏡の照明装置。   An illuminating device having a light emitting unit for illuminating an observation sample in an optical microscope, the illuminating device used for the optical microscope having an objective lens and an observation sample supporting unit, comprising a base unit, a power source, a switch, and a movable arm And the light emitting unit is arranged in a movable state in the vicinity of the observation sample of the observation sample support unit, and when used for bright field system observation, the light emitting unit of the objective lens In the optical axis, arranged on the opposite side of the objective lens with respect to the objective sample support part, and when illuminating the illumination light from the light emitting part toward the observation sample, from below the observation sample support part, An illumination device for an optical microscope, wherein transmitted light from the observation sample is incident on the optical axis of the objective lens and facilitates bright field observation. 光学顕微鏡において観察試料を照明するための発光部を有する照明装置で、対物レンズと、観察試料支持部とを有する前記光学顕微鏡に用いる照明装置であって、ベース部、電源、スイッチ、可動型アームおよび発光部を有し、前記発光部は前記観察試料支持部の前記観察試料の近傍に移動可能な状態で配置され、通常の透過型光学顕微鏡を実体顕微鏡として使用する場合には、前記発光部は前記観察試料支持部に対して前記対物レンズと同じ側に配置され、前記発光部からの照明光を前記観察試料に向けて、前記観察試料支持部の斜め上方から照射すると、前記観察試料からの反射光は前記対物レンズの光軸に入射され、実体観察が容易にできる事を特徴とする光学顕微鏡の照明装置。   An illuminating device having a light emitting unit for illuminating an observation sample in an optical microscope, the illuminating device used for the optical microscope having an objective lens and an observation sample supporting unit, comprising a base unit, a power source, a switch, and a movable arm And the light emitting part is arranged in a movable state in the vicinity of the observation sample of the observation sample support part, and when using a normal transmission optical microscope as a stereomicroscope, the light emitting part Is arranged on the same side as the objective lens with respect to the observation sample support part, and when the illumination light from the light emitting part is directed to the observation sample from obliquely above the observation sample support part, The optical microscope illumination device is characterized in that the reflected light is incident on the optical axis of the objective lens and can be easily observed. 光学顕微鏡のための照明装置が、請求項1、請求項2および請求項3に記載の装置を有し、スイッチの切り替えにより、選択的に、暗視野系、明視野系および実体観察系照明の点灯が容易にできる事を特徴とする光学顕微鏡の照明装置。   An illumination apparatus for an optical microscope has the apparatus according to claim 1, claim 2, and claim 3, and selectively switches dark field system, bright field system and entity observation system illumination by switching a switch. An illumination device for an optical microscope, which can be easily turned on.
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JPWO2013094434A1 (en) * 2011-12-22 2015-04-27 パナソニックヘルスケアホールディングス株式会社 Observation system, control method and program for observation system

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2013094434A1 (en) * 2011-12-22 2015-04-27 パナソニックヘルスケアホールディングス株式会社 Observation system, control method and program for observation system
US9025246B2 (en) 2011-12-22 2015-05-05 Panasonic Healthcare Holdings Co., Ltd. Observation system and method of controlling observation system

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