JP3097642U - Diamond knife for optical microscope and electron microscope - Google Patents

Abstract

An object of the present invention is to easily and reliably remove section waste and unnecessary sections from the water surface of a knife boat.
A step 2 for fixing a suction pipe is provided above a diamond knife.
[Selection diagram] Fig. 1

Description

[0001]
[Technical field to which the invention belongs]
The present invention relates to a diamond knife used for an optical microscope and an electron microscope sample, which is used when a biological sample is fixed, dehydrated, and embedded, and then a section is prepared with an ultramicrotome.
[0002]
[Prior art]
The tissues and cells that make up living organisms contain a large amount of water.When trying to observe the microstructure of a sample collected by cell function observation, etc. with an electron microscope, ethanol and other materials are used to fix the sample and remove water. And then immersed in a resin and embedded in the resin to produce ultra-thin sections with an ultramicrotome.
[0003]
In the slicing operation, a thin slice is prepared by cutting with an ultramicrotome using a diamond knife or a glass knife.
The diamond knife is provided with a boat so that the cut pieces can be temporarily stocked while floating on the surface of the boat.
At this time, section waste and unnecessary sections that cannot be used for electron microscopic observation are generated.
[0004]
[Problems to be solved by the invention]
Conventionally, section waste and unnecessary sections that cannot be used for electron microscopic observation have been removed by suction with a knife boat water level controller equipped with a pump, or unnecessary sections have been removed manually one by one with a pine wool probe. The knife boat water level adjustment device is expensive and requires a power supply, and the removal work with a pine hair probe has the problem that it is very troublesome because unnecessary pieces are removed one by one while looking through the binocular loupe of the ultramicrotome. .
[0005]
The present invention has been made in view of the above-mentioned circumstances, and an object thereof is to provide a diamond for an optical microscope and an electron microscope which can easily and efficiently remove section waste and unnecessary sections on a knife boat water surface. There is a knife to offer.
[0006]
Means and action for solving the problem
This invention is characterized in that in order to achieve the above-mentioned object, a fixed step for a suction pipe is provided on a side surface inside the boat above the knife, so that it is possible to easily remove section waste and unnecessary sections on the boat water surface.
[0007]
According to the above configuration, a suction pipe such as a thin section removing tool can be fixed to the step on the side surface in the knife upper boat, and the section waste and unnecessary sections floating on the water surface by sucking only the boat water surface. Can be removed.
[0008]
[Embodiment of the invention]
Hereinafter, embodiments of the present invention will be described with reference to the drawings.
[0009]
FIG. 1 is a perspective view showing a diamond knife for an optical microscope and an electron microscope, and FIG. 2 is a perspective view showing a state in which a section floating on the water surface is removed by a suction pipe.
At the upper part of the knife body 1, there is provided a boat section 4 capable of floating sliced slices on the surface of distilled water 7 and temporarily stocking them. A knife portion 3 for cutting is formed at the top of the boat portion 4. A suction pipe fixing step 2 is formed on both sides and the inside rear part in the boat.
Next, the operation will be described. When the end portion of the resin-embedded sample is pressed against the knife portion 3 with an ultramicrotome and pressed downward, a slice 6 that is sliced and floated on the water surface is obtained. At this time, section scraps and unnecessary sections that cannot be observed with a microscope are generated. The cut piece and the unnecessary piece floating on the water surface are fixed to the suction pipe fixing step 2 with the suction pipe 5, and the piece piece and the unnecessary piece on the water surface are removed by suction, and then a section for observation is prepared.
Further, the step 2 for fixing the suction pipe is not limited to being provided in almost the entire area above the boat, and a local step 8 may be provided as shown in FIG. Further, it may be provided on a boat for a glass knife.
[0011]
[Effect of the invention]
As described above, according to the present invention, by providing the step for fixing the suction pipe, it is possible to easily and efficiently remove the piece debris and unnecessary pieces floating on the water surface of the knife boat, thereby improving operability and economy. There is an effect that can be improved.
[Brief description of the drawings]
FIG. 1 is a perspective view of a diamond knife for an optical microscope and an electron microscope. FIG. 2 is a perspective view showing a state in which a section floating on the water surface is removed by a suction pipe. FIG. 3 shows another embodiment of the present invention. Perspective view showing [Description of symbols]
1: Knife body 2: Pipe fixing step

Claims (1)

The knife body consisting of a metal block, the entire upper part of this knife body is a boat for holding distilled water, and the section cutting knife section provided on the top of this boat, the side water inside the boat is filled with surface water A diamond knife for an optical microscope and an electron microscope, which has a step for fixing a suction pipe at a position one step lower than the water surface so as to be easily sucked by a suction pipe or the like.

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