CN109085008A - A kind of culture dish free-hand section method of dry vine tea leaf - Google Patents
A kind of culture dish free-hand section method of dry vine tea leaf Download PDFInfo
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- CN109085008A CN109085008A CN201811030580.3A CN201811030580A CN109085008A CN 109085008 A CN109085008 A CN 109085008A CN 201811030580 A CN201811030580 A CN 201811030580A CN 109085008 A CN109085008 A CN 109085008A
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- tea leaf
- blade
- vine tea
- culture dish
- water
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of culture dish free-hand section method of dry vine tea leaf, the method will be placed in culture dish by the vine tea leaf of immersion, sofening treatment, and is immersed in water；Vine tea leaf is cut in the horizontal direction using blade again, finally uses the micro- sem observation of aqueous film-making.The method of the present invention is easy to operate, at low cost, time-consuming short, and blade can be made to fix well, to cut out complete chip-proof purpose thin slice.
Description
Technical field
The present invention relates to field of biotechnology, and in particular to a kind of culture dish free-hand section method of dry vine tea leaf.
Background technique
Free-hand section technology is common basic experiment technology during Botany Experiment, can cut fresh material, observe
To plant tissue natural colour and living structures, using wide hair in Botany Experiment teaching and scientific research.This method is usually used in studying
Plant anatomy structure, Histochemistry stain ingredient, plant resources identification etc..Generally for small, soft, excess moisture and heavily fortified point
Hard material, it is difficult to free-hand section.Vine tea blade belongs to wherein softer a kind of material, has document report aobvious to vine tea leaf
Microstructure observation, mainly using paraffin section and the dicing method that slips；The paraffin section period is longer, through chemistry in slicing processes
Certain chemical components of loss cell or tissue are comprehended in fixed, dehydration, transparent, embedding etc.；And microtomy of slipping, it is mainly used for
Harder material is cut, blade need to be clamped with mechanical device in cutting, then cut to object, be easy when cutting vine tea leaf
Leaf is cut bad.
Summary of the invention
In order to solve problems in the prior art, the purpose of the present invention is to provide a kind of cultures of dry vine tea leaf by the present invention
Ware free-hand section method, the dicing method is simple, strong operability, and the vine tea leaf blade construction tissue cut out is complete, convenient for seeing
It examines.
To solve the above problems, the present invention provides a kind of culture dish free-hand section method of dry vine tea leaf, the method
The following steps are included:
1) the vine tea leaf of several dryings is taken；
2) it impregnates through water, sofening treatment, is trimmed with scissors along master pulse two sides after taking out blade；
3) by the above-mentioned blade material handled well, tiling is put into culture dish, adds clear water until clear water submerges culture dish bottom；
4) upper plastic cement fingerstall is covered on finger, and pins the vine tea leaf with the finger with plastic cement fingerstall；
5) blade is held with another to be close to pin the finger of the vine tea leaf, blade parallel is grasped in vine tea leaf direction, from
Blade hand one end is slowly tangentially held in pressing hand one end, continuously cuts 3-5 times, blade is slided in clear water, make to be sticked to blade blade
Slice is immersed in clear water；
6) clear water is dripped in the center of glass slide with rubber head dropper；
7) it is selected using the tip of dissecting needle and swims in the culture dish water surface, transparent leaf sections, be placed in the water of the glass slide
In drop, puts and carry out microexamination on coverslip.
Further, vine tea leaf is impregnated using 90-98 DEG C of water in step 2, sofening treatment.
Further, in step 3), the vine tea leaf after cutting trimming retains 2-4mm along master pulse two sides respectively, and blade is long
1.5-3cm。
Further, the blade in cutting on the outside of the pressing finger at 2-3mm cut.
Beneficial effects of the present invention: the production method of vine tea leaf sections of the invention, it is easy to operate, at low cost, time-consuming
It is short, blade can be made to fix well, so that the vine tea leaf blade texture cut out is not crushed completely, convenient for observation.
Specific embodiment
The present invention will be described in detail combined with specific embodiments below.
The culture dish free-hand section method of dry vine tea leaf provided by the invention the following steps are included:
1) the vine tea leaf of several dryings is taken；
2) it impregnates through water, sofening treatment, is trimmed with scissors along master pulse two sides after taking out blade；
3) by the above-mentioned blade material handled well, tiling is put into culture dish, adds clear water until clear water submerges culture dish bottom；
4) upper plastic cement fingerstall is covered on finger, and pins the vine tea leaf with the finger with plastic cement fingerstall；
5) blade is held with another to be close to pin the finger of the vine tea leaf, blade parallel is grasped in vine tea leaf direction, from
Blade hand one end is slowly tangentially held in pressing hand one end, continuously cuts 3-5 times, blade is slided in clear water, make to be sticked to blade blade
Slice is immersed in clear water；
6) clear water is dripped in the center of glass slide with rubber head dropper；
7) it is selected using the tip of dissecting needle and swims in the culture dish water surface, transparent leaf sections, be placed in the water of the glass slide
In drop, puts and carry out microexamination on coverslip.
Rattan tealeaves blade is fixed with hand using the culture dish free-hand section method of dry vine tea leaf provided in this embodiment
In the culture dish bottom being filled with water, blade is made to be well fixed, the organization of vine tea leaf will not be destroyed, it can be to greatest extent
Holding vine tea leaf integrity degree.
It prevents material when slice from pasting on blade with the operation of water immersion vine tea leaf, guarantees the validity of cutting, improve
The efficiency of cutting.
The dicing method, it is easy to operate, at low cost, time-consuming short, blade can be made to fix well, the rattan tealeaves blade cut out
Institutional framework is not crushed completely, convenient for observation.
Embodiment 1
During further experiment is with adjustment, inventors have found that the above method is advanced optimized as following procedure, it can
It saves rattan tealeaves blade more preferable, is more convenient cutting:
A kind of culture dish free-hand section method of dry vine tea leaf, comprising the following steps:
1) dry vine tea leaf is taken；
2) it is impregnated with 95 DEG C of water, appropriate sofening treatment, softening degree is cut with knife to be thinly sliced and section is completely advisable；Take out leaf
It is trimmed with scissors along master pulse two sides after piece, master pulse two sides retain 2mm, the long 2cm of blade respectively；
3) by the above-mentioned blade material handled well, tiling is put into the culture dish of diameter 9cm, adds suitable clear water, to submerge training
Supporting ware bottom is advisable；
4) blade is pinned with the left index finger with disposable plastic cement fingerstall；
5) right hand holds double-edged razor blade and is close to index finger with parallel direction from left side one end slowly tangential the right one end, continuously cuts 3 times, will
Blade slides in clear water, makes to be sticked to blade leaf sections submerged in water；
6) clear water is dripped in the center of glass slide with rubber head dropper；
7) it is selected using the tip of dissecting needle and swims in the culture dish water surface, transparent leaf sections, be placed in the water of above-mentioned glass slide
It is put in drop, microexamination is carried out in covered.
Embodiment 2
The present embodiment is distinguished as with embodiment 1, blade in cutting on the outside of the pressing finger at 2-3mm cut.In this way
The benefit done is firmly uniformly, and to cut uniform in size.
In the above method, one hand of operator presses vine tea leaf, and another holds blade cutting, with the strong hand of operator
Subject to, it is not limited to the mode of operation that left hand presses right hand-held knife.
There are many concrete application approach of the present invention, the above is only a preferred embodiment of the present invention, it is noted that for
For those skilled in the art, without departing from the principle of the present invention, it can also make several improvements, this
A little improve also should be regarded as protection scope of the present invention.
Claims (4)
1. a kind of culture dish free-hand section method of dry vine tea leaf, which is characterized in that the described method comprises the following steps:
1) the vine tea leaf of several dryings is taken；
2) it impregnates through water, sofening treatment, is trimmed with scissors along master pulse two sides after taking out blade；
3) by the above-mentioned blade material handled well, tiling is put into culture dish, adds clear water until clear water submerges culture dish bottom；
4) upper plastic cement fingerstall is covered on finger, and pins the vine tea leaf with the finger with plastic cement fingerstall；
5) blade is held with another to be close to pin the finger of the vine tea leaf, blade parallel is grasped in vine tea leaf direction, from
Blade hand one end is slowly tangentially held in pressing hand one end, continuously cuts 3-5 times, blade is slided in clear water, make to be sticked to blade blade
Slice is immersed in clear water；
6) clear water is dripped in the center of glass slide with rubber head dropper；
7) it is selected using the tip of dissecting needle and swims in the culture dish water surface, transparent leaf sections, be placed in the water of the glass slide
In drop, puts and carry out microexamination on coverslip.
2. a kind of culture dish free-hand section method of dry vine tea leaf according to claim 1, which is characterized in that step 2
In vine tea leaf is impregnated using 90-98 DEG C of water, sofening treatment.
3. a kind of culture dish free-hand section method of dry vine tea leaf according to claim 1, which is characterized in that step 3)
In, the vine tea leaf after cutting trimming retains 2-4mm, the long 1.5-3cm of blade along master pulse two sides respectively.
4. a kind of culture dish free-hand section method of dry vine tea leaf according to any one of claims 1 to 3, feature exist
When, the blade is in cutting on the outside of the pressing finger at 2-3mm cut.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811030580.3A CN109085008A (en) | 2018-09-05 | 2018-09-05 | A kind of culture dish free-hand section method of dry vine tea leaf |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811030580.3A CN109085008A (en) | 2018-09-05 | 2018-09-05 | A kind of culture dish free-hand section method of dry vine tea leaf |
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| CN109085008A true CN109085008A (en) | 2018-12-25 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112881112A (en) * | 2021-01-07 | 2021-06-01 | 河南科技大学 | In-situ microdissection method for sweet potato fruits |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107014809A (en) * | 2017-04-19 | 2017-08-04 | 安徽科技学院 | The quick free-hand section method of master pulse support blade |
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2018
- 2018-09-05 CN CN201811030580.3A patent/CN109085008A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107014809A (en) * | 2017-04-19 | 2017-08-04 | 安徽科技学院 | The quick free-hand section method of master pulse support blade |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112881112A (en) * | 2021-01-07 | 2021-06-01 | 河南科技大学 | In-situ microdissection method for sweet potato fruits |
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Application publication date: 20181225 |
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| RJ01 | Rejection of invention patent application after publication |