JP3093205B1 - Method and apparatus for producing mycelial agglomerate extract of Agaricus blazei muril - Google Patents

Abstract

Abstract: PROBLEM TO BE SOLVED: To produce a high-quality Agaricus blazei muril mycelium mass extract in a considerably shorter number of days than in the past. SOLUTION: A granular mycelium A is stirred and mixed by a stirring blade 17 while being subjected to aeration for a predetermined number of days together with water at room temperature in a basket container 12 having a filter 13 fixed at an upper part in a culture tank 10 while being aerated. , Agaricus brazei muril mycelium mass extract is extracted while being cultured,
Next, it is introduced into the sterilizing tank 22, heated and sterilized while being aerated, and then introduced into the storage tank 36 and stored until aerated while being shipped.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to agaricus mushrooms (Japanese name is agaricaceae, agaricus genus Kawariharatake).
And the scientific name is Agaricus blazei Murir (Aga
ricus blazei Murill) and an apparatus for producing the same.

[0002]

2. Description of the Related Art Agaricus mushrooms are known to have an anticancer effect, an anticancer effect, a cancer prevention effect, a blood sugar lowering effect, a blood pressure lowering effect, a decholesterolizing effect and an improving effect on arteriosclerosis. In recent years, it has been used as a health and nutritional food. Conventionally, agaricus mushrooms did not remain artificially cultivated, but as a result of extensive research and trial and error, cultivation was successful and mass production became possible.

[0003] In other words, Agaricus mushrooms are prepared by first fermenting Bacus, mixing it with soil to form a bacterial bed, then heat-treating the bacterial bed by heating at a high temperature, and inoculating the bacteria in a sterile state. The mycelium is cultured to generate Agaricus mushrooms. The agaricus mushrooms are dried immediately after harvesting, and the dried agaricus mushrooms are processed into health foods such as extracts and granules in a food factory.

[0004] Since dried agaricus mushrooms are very expensive and difficult to obtain, the mycelium of Agaricus brazei muril is directly cultured without using the dried agaricus mushrooms and produced as an extract. The method is adopted.

The method for producing the mycelium extract of Agaricus blazei muril includes the following methods:
Culturing the mycelium of muril and developing the mycelium of Agaricus blazei Murrill, Agaricus blazei
After the mycelium of muril starts to separate from the medium, the stage of growing the mycelium of Agaricus blazei muril, adjusting the mycelium of propagated Agaricus brazei muril, adjust the extract in the desired state A method including a step is known (for example, see Japanese Patent No. 2887756).

[0006]

However, the manufacturing method according to Japanese Patent No. 2887756 requires not only a considerably long time (about 12 to 23 days) but also practically Agaricus brazei Murrill at the time of manufacturing. The mycelium is put into a cloth net bag, and the agaricus brazei muril mycelium extract is extracted while aerating while hanging it in a tank.Therefore, oxygen is sufficiently introduced into the mycelium even by aeration. If not supplied, fermentation and spoilage occur, and the produced liquid (extract) has an unpleasant odor, and there is a problem that the number of living bacteria varies and the quality is poor.

The present invention has been made in view of the above-mentioned problems of the prior art, and has as its object to be able to produce it quickly (about 6 to 7 days) and to reduce the number of viable bacteria. It is an object of the present invention to provide a method and a device capable of producing a mycelial agglomerate of Agaricus blazei muril, which is high in quality with no variation and without unpleasant odor and which is optimal as a health nutritional food.

[0008]

Means for Solving the Problems To achieve this object, the invention according to claim 1 is characterized in that fine granules placed in a fixed basket with a filter are fixed in the upper part of the culture tank into which the normal temperature water is introduced. Alternatively, the granular Agaricus blazei muril mycelial mass is stirred and mixed with Myosui for 3 days while being aerated from the bottom of the culture tank in a closed basket with a filter of the culture tank. A first step in which the Muryl mycelium mass extract is extracted while being cultured, and the liquid obtained in the first step is introduced into a sterilization tank while being filtered, and the liquid temperature is 100 ° C. in the sterilization tank. At this temperature
A second step in which the bacteria in the liquid are sterilized by holding for 5 minutes, aeration is continued until the temperature reaches room temperature to remove suspended matters such as bubbles and dead bacteria from the liquid, and A third step in which the temperature is raised to 100 ° C., held at this temperature for 15 minutes and sterilized again, aeration is continued until the temperature reaches room temperature, and suspended matter from the liquid is removed again, and the third step is obtained. The liquid is introduced into the storage tank while being filtered and stored in the storage tank while being subjected to aeration, whereby a mycelial mass extract of Agaricus blazei muril is obtained. It is a feature.

[0009] The invention according to claim 2 is characterized in that the sterilizing tank has a volume approximately half that of the culture tank and is connected to and arranged in parallel with the culture tank. It is characterized in that the second and third steps are performed.

[0010] The invention according to claim 3 is the second and third inventions.
In the process, aeration is repeated for one hour at intervals of 5 minutes, and the temperature of the solution is raised to 100 ° C.

According to a fourth aspect of the present invention, there is provided a basket container with a filter, which is internally provided at an upper portion, a stirring means which rotates in the basket container with a filter under the rotational force of a driving source, and which is provided near the bottom. Aeration means, and a closed lid for opening and closing the top opening, and the fine-grained or granular Agaricus blazei muril mycelium mass put into the basket with a filter is subjected to aeration by the aeration means. The culture tank is stirred and mixed by the stirring means while receiving, and a culture tank from which the Agaricus blazei muril mycelial mass extract is extracted while being cultured, is connected to the culture tank via a pipe and a suction pump, and has an upper opening. A sealing lid that opens and closes, and a heating unit and an aeration unit inside, and the aeration unit in which the liquid temperature of the liquid introduced after being filtered from the culture tank is intermittently repeated. While receiving the aeration, the sterilization tank is heated to 100 ° C. by the heating means to sterilize various bacteria in the liquid. The sterilization tank is connected to the sterilization tank via piping and a suction pump, and opens and closes an upper opening. A sealing lid,
A storage tank having an aeration means therein, and a storage tank for temporarily storing liquid filtered and introduced from the inside of the sterilization tank until it is shipped as a product while being aerated by the aeration means. It is a feature.

According to a fifth aspect of the present invention, the sterilization tank has a volume approximately half that of the culture tank, and two of the sterilization tanks are connected to the culture tank in parallel via a pipe and a suction pump. Is characterized by the following configuration.

[0013]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Embodiments of the present invention will be described with reference to the drawings and the operation thereof. FIG. 1 is a system diagram of an example of the production apparatus according to the present invention. First, as a first step, Agaricus blazei muril mycelium mass is made as fine as possible, finely granular or Granular (hereinafter referred to as granular mycelium),
This granular mycelium A is stirred and mixed with normal water at room temperature in the culture tank 10 to extract the Agaricus blazei muril mycelial mass extract while culturing.

More specifically, the culture tank 10 is a cylindrical body having excellent corrosion resistance and made of a metal such as stainless steel and having a circular bottom at the bottom.
It is erected at a location via a welding support leg (not shown). At the upper part of the inner peripheral wall of the culture tank 10, 4
One of the receiving trays 11 is horizontally fixed inward, and on each of these four receiving trays 11 is placed a basket container 12 having a cylindrical shape and a filter 13 having an upper surface opening. The filter 13 has a fine mesh and is stretched on the container peripheral surface and the bottom surface excluding the upper opening surface, and is detachable so that it can be washed and replaced.

At the center of the basket container 12, a rotating shaft 16 is provided via a bearing 15 from a sealing lid 14 for opening and closing the tank upper surface, and a stirring blade 17 and a sealing lid connected to the lower end of the rotating shaft 16. Drive motor 1 for rotating shaft 16 disposed on
8 is provided.

Into the culture tank 10, about 10 liters of water at room temperature obtained by a Myosui water activating machine (not shown) is introduced through an upper inlet of the culture tank 10. About 1.1 to 1.2 kg of granular mycelium A is charged therein. Next, the mycelium A was sealed with the sealing lid 14 and the granular mycelium A together with Myosui was placed in the basket 1 with a filter.
2, the agitation blade 17 is rotated by the rotation force of the motor 18 and agitated and mixed at a speed of about 30 rotations per minute for 3 days while being aerated from the central portion close to the tank bottom. The extract of Brassii muril mycelium mass is extracted while being cultured.

As shown in FIG. 2, the aeration means includes a pipe 19 fixedly inserted through the side wall so that the downward blow-off port 20 is located at a central portion close to the inside of the tank bottom. It includes a compressor 21 connected to the pipe 19, and the compressed air is blown downward from the outlet 20 through the pipe 19 by the operation of the compressor 21 to be aerated.

The liquid in the culture tank 10 is introduced into the sterilization tank 22 while being filtered on the fourth day, and is shifted to the second step.

The sterilizing tank 22 is a cylindrical body having excellent corrosion resistance, for example, made of metal such as stainless steel and having an arc-shaped bottom, and has welding support legs (not shown) on four outer walls. It is erected through. The sterilization tank 22 may be one having substantially the same volume as the culture tank 10, but is preferably two as shown in FIG. 1, having approximately half the volume of the culture tank 10. The sterilization tanks 22 are preferably connected and arranged in parallel. This is to increase the efficiency of heating by a heater described later to reduce the processing time.

The structure of the two sterilizing tanks 22 is the same, each has a sealing lid 23 for opening and closing the upper opening, and has a downward blowing port 20 equivalent to the aeration means in the culture tank 10 inside. A piping 19 having the aeration means including a compressor 21 and a heater 24 as a heating means are additionally provided. As illustrated in FIG. 3, the heater 24 penetrates a tank outer wall near the tank bottom, and a socket 25 is fixed to the heater 24, and a heater member 26 including a plurality of heater rods is fixed to the socket 25. The liquid is heated to a predetermined temperature by the heater 24 by being screwed in such a manner that the liquid is horizontal and the front end is positioned substantially at the center of the tank.

As described above, the liquid from the culture tank 10 is introduced into the sterilization tank 22.
As shown in FIG. 4, the liquid in the suction pipe 27 is opened at a position slightly higher than the bottom of the tank, bent into a substantially L-shape, and welded through the outer wall surface of the tank. The suction at the bottom of the tank is sucked by the operation of the suction pump 29 via the pipe 28 connected to the pipe 27 so as not to suck up the sediment.
0, two sterilization tanks 22 via an upright pipe 31 connected to the pipe 30 and an upright pipe 32 branched from the pipe 30
The same amount is introduced into each. The liquid outlet 33 of the upright pipes 31 and 32 in the two sterilization tanks 22 has
As illustrated in FIG. 5, a cartridge type filter 34 is screwed, and a ball valve 35 is attached to each of the pipes 28 and 30 and the upright pipe 32. By opening and closing these valves 35, The liquid from the culture tank 10 passes through each pipe and is introduced into the two sterilization tanks 22 while being filtered by the filter 34.

More specifically, in the second step, the liquid in the two sterilization tanks 22 that has been filtered and transferred from the culture tank 10 on the fourth day is supplied to the heater switch with timer in the closed tank. The liquid temperature is raised to 100 ° C. over about 16 hours by the heater 24 by being turned on, and the liquid temperature of 100 ° C. is maintained for about 15 minutes to sterilize various bacteria in the liquid. In addition, the liquid temperature is 100 ° C.
When the air is raised, the compressed air obtained by the compressor 21 as the aeration means is blown out from the air outlet 20 to about 1
By repeating the aeration for about 5 minutes at time intervals, the room temperature layer and the heating layer in the liquid are mixed, the temperature distribution in the liquid is made uniform, and the temperature rise can be measured quickly. When the liquid temperature rises to 100 ° C, foreign bodies such as dead corpses of dead bacteria and bubbles float, so these suspended matters are frequently removed and aeration is continued for about 24 hours until the liquid temperature reaches room temperature. During this time, suspended matter is frequently removed.

Next, as a third step, in the same manner as in the second step, in the two sterilization tanks 22, the aeration of the liquid temperature at room temperature is repeated by the heater 24 at intervals of about 1 hour for about 5 minutes. 100 hours again for about 14 hours
℃, and at this liquid temperature of 100 ℃, about 1
After being held for 5 minutes, re-sterilization is performed, and then aeration is continued for about 24 hours until the room temperature is reached by turning off the heater 24, during which the suspended matter is removed again.

Next, as a fourth step, the liquid in the two sterilization tanks 22 is introduced into the storage tank 36.

The storage tank 36 is a cylindrical body having excellent corrosion resistance and made of a metal such as stainless steel and having an arcuate bottom, and has welding support legs (not shown) on four outer walls. The storage tank 36 has a closed lid 37 for opening and closing the upper surface opening, and has therein a pipe 19 having a downward outlet 20 equivalent to the aeration means in the culture tank 10. An aeration unit including a compressor 21 connected to the pipe 19 and a preliminary heater 24 serving as a preliminary heating unit are additionally provided.

As in the case of the culture tank 10, the liquid in the two sterilization tanks 22 is opened slightly above the bottom of the tank, and is bent and formed into a substantially L-shape to form an outer wall surface of the tank. The suction pipe 27, which is welded through the pipe 27, is connected to a common pipe 38 via a pipe 28 connected to the pipe 27, and the suction pump 39 connected to the common pipe 38 is operated to remove the sediment at the bottom of the sterilization tank. It is sucked upward from below so as not to suck up.
9 and a storage tank 36 while being filtered by a cartridge type filter 34 mounted on an upper liquid inlet 42 through a pipe 40 connected to the pipe and an upright pipe 41 from the pipe.
And is temporarily stored while being aerated by the aeration means and, if necessary, heated by the preliminary heater 24 until it is shipped as a product.

When the product is shipped from the storage tank 36, the product is opened slightly above the bottom of the storage tank and bent into a substantially L-shape, similarly to the transfer from the sterilization tank 22 to the storage tank 36. A suction pipe 27 formed and welded through the outer wall surface of the tank and a pipe 28 connected to the pipe 27 are operated upward by a suction pump 42 so as not to suck up sediment at the bottom of the storage tank. It is aspirated and sent to a bottling room where it is bottled and shipped as a product.

A drain port at the center of the bottom of the culture tank 10, the two sterilizing tanks 22 and the storage tank 36 is connected to a common drain pipe 44 through each pipe 43 and a ball valve 35, and the residual water in each tank is removed. The liquid, washing water in the tank, and the like are drained to the outside by opening and closing each ball valve 35.

As described above, the mycelial mass extract of Agaricus blazei muril is produced through the first to fourth steps, and all the steps are performed in a clean room in about 6 to 7 days. Also, bottling work is also performed in a clean room.

In the examples described above, the raw materials for extract extraction were all made of Agaricus blazei muril mycelium mass. The mycelial mass was mainly used, and the raw material was subjected to raw or drying treatment. Agaricus mushrooms may be minced and mixed.

[0031]

Thus, according to the present invention, fine or granular Agaricus blazei spores are placed in a fixed filter basket at the top of the culture tank into which the normal temperature of Myosui is introduced. The mycelial agglomerates of Agaricus blazei muril are stirred and mixed with myowater for 3 days while being aerated from the bottom of the culture tank in a closed basket with a filter of the culture tank. Since it is extracted while being cultured, the cultivation of the mycelial mass extract of Agaricus blazei muril is quick, and because it is aerated while stirring and mixing, oxygen is sufficient in the mycelium, and High quality agar with good reliability, no fermentation and decay of mycelium as in the past, with a large number of viable bacteria, no variation, and no unpleasant odor. Mycelial masses extract of the box blazei Murill can be produced in a short number of days of only 6-7 days.

[Brief description of the drawings]

FIG. 1 is a system diagram of an example of an apparatus for producing a mycelium mass extract of Agaricus blazei muril according to the present invention.

FIG. 2 is a partially omitted longitudinal sectional view showing a configuration of an aeration unit.

FIG. 3 is a partially omitted longitudinal sectional view showing a configuration of a heating unit.

FIG. 4 is a partially omitted longitudinal cross-sectional view showing a liquid suction and lead-out structure in a culture tank, two sterilization tanks, and a storage tank.

FIG. 5 is a partially omitted longitudinal sectional view showing a filtration structure of a liquid inlet in two sterilization tanks and a storage tank.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 10 Culture tank 11 Cradle 12 Basket container 13 Filter 14 Sealing lid 15 Bearing 16 Rotating shaft 17 Stirring blade 18 Drive motor 19 Piping 20 Blow outlet 21 Compressor 22 Sterilization tank 23 Sealing lid 24 Heater 27, 28, 30, 38, 40 Piping 29, 39, 42 Suction pump 31, 32, 41 Standing pipe 33 Liquid outlet 34 Filter 35 Valve 36 Storage tank 37 Sealing lid A Granular mycelium

Claims (5)

(57) [Claims] Claims 1. A fine or granular Agaricus blazei muril mycelial mass put into a stationary basket with a filter in the upper part of a culture tank into which a normal temperature water is introduced is sealed and cultured. A first step in which the agaricus brazei muril mycelial mass extract is extracted while being cultured while being stirred and mixed with myowater for 3 days while being aerated from the bottom of the culture tank in a filter basket container of the tank; The liquid obtained in the first step is introduced into a sterilization tank while being filtered, and in the sterilization tank, the liquid temperature is raised to 100 ° C. and maintained at this temperature for 15 minutes to remove various bacteria in the liquid. A second step in which sterilization is performed and aeration is continued until the temperature reaches room temperature to remove suspended matters such as bubbles and dead bacteria from the liquid, and the liquid temperature is raised to 100 ° C. again. A third step in which the liquid is held at this temperature for 15 minutes to be sterilized again, aeration is continued until the temperature reaches room temperature to remove suspended matters from the liquid again, and the liquid obtained in the third step is stored while being filtered. A fourth step in which the mycelial mass extract of Agaricus blazei muril is obtained by being introduced into the tank and stored in the storage tank while being subjected to aeration, thereby obtaining an Agaricus blazei. A method for producing a myllium mass extract of muril. 2. The sterilization tank has a volume approximately half that of the culture tank and is connected in parallel with the culture tank. Two of the sterilization tanks are disposed in the two sterilization tanks. 2. The method for producing a mycelial agglomerate extract of Agaricus blazei muril according to claim 1, wherein the step (a) is performed. 3. The Agaricus blazei according to claim 1, wherein in the second and third steps, aeration is repeated for one hour at intervals of 5 minutes to raise the liquid temperature to 100 ° C. -A method for producing a mycelial mass extract of muril. 4. A basket container with a filter, which is internally fixed at an upper portion, a stirring means which rotates by receiving a rotational force of a driving source in the basket container with a filter, aeration means provided near the bottom, and an upper surface. A closed lid that opens and closes the opening, and the fine-grained or granular Agaricus blazei muril mycelium mass put in the basket with filter is subjected to the aeration by the aeration means while being aerated by the aeration means. A culture tank that is stirred and mixed to extract the Agaricus blazei muril mycelial mass extract while being cultured, and a closed lid that is connected to the culture tank via piping and a suction pump, and that opens and closes an upper opening. Inside, it has a heating means and an aeration means, and while the liquid temperature of the liquid filtered and introduced from the culture tank is aerated by the aeration means which is intermittently repeated. 100 ° C. by the heating means
A sterilization tank that is raised to and sterilizes germs in the liquid; a sterilization tank connected to the sterilization tank and piping, a suction pump, and a closed lid that opens and closes an upper opening; and an aeration unit inside, Liquid introduced by filtration from the sterilization tank,
An apparatus for producing an Agaricus blazei muril mycelium mass extract, comprising: a storage tank that is temporarily stored until it is shipped as a product while being aerated by the aeration means. 5. The agaricus according to claim 4, wherein the sterilization tank has a volume approximately half that of the culture tank, and two of the sterilization tanks are connected in parallel to the culture tank via a pipe and a suction pump.・ Brazy Muryl mycelial mass extract manufacturing equipment.