JP3082912B2 - Livestock egg maturation, fertilization, developmental incubator - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a transportable mature incubator which matures, fertilizes, and develops eggs of livestock such as cattle, horses, pigs, etc., and produces transplantable embryos.

[0002]

2. Description of the Related Art Conventionally, ova used for producing in vitro fertilized embryos of cattle are obtained by removing ovaries from cattle slaughtered in a slaughterhouse, placing the ovaries in a preservative solution, and bringing them back to a processing room where the immature ova are extracted from the ovaries. It is a fact that maturation, in vitro fertilization and extracorporeal development occur at a temperature of 38.5 ° C. and 5% CO 2 gas phase.

[0003] The produced in vitro fertilized eggs are transported in a state of being stored in a preservative solution, sealed in a transport container, a straw or the like. As a culture transporter used at this time, a battery and a heater are built in a container having an insulated structure, and livestock embryos are adjusted in a processing room equipped with instruments and equipment and stored in a straw tube. To transport.

[0004]

However, eggs of livestock are easily affected by environmental changes, and in the above-mentioned conventional method, the longer the transport distance from the slaughterhouse to the processing room, the more immature the ovaries will be. Eggs will lose their ability to survive and grow, and in the worst case, they will die during and after transportation, or will have a problem that they will not be able to be used for their intended purpose, even if they do not die. .

[0005] The present invention has been made in view of the above-mentioned problems of the prior art.
It is an object of the present invention to provide a simple incubator capable of carrying ovum collected from the ovaries of livestock taken out at a slaughterhouse while being cultured, fertilizing, developing and culturing in a vibrant state, and transporting the ovary to a destination. Another object is to provide a simple incubator which can be provided at a low cost with a very simple structure.

[0006]

Means for Solving the Problems The technical measures taken by the present invention to solve the above-mentioned problems include a petri dish containing an immature egg (hereinafter referred to as an egg) taken out of the ovary of a domestic animal together with a preservative solution. A valve provided with a check valve for connecting a suction device that makes the inside of the container body a negative pressure is attached to a portable container body having a closed lid that can be taken in and out, and the inside of the container body is carbonated. by injecting Gasupauda chromatography, characterized in that the predetermined carbon dioxide concentration.

The above-mentioned container body comprises a container and a lid provided with a sealable seal mechanism. The container and the lid may be made of synthetic resin or metal. When made of synthetic resin, the inside of the container body is made transparent or translucent so that a chemical for generating carbon dioxide (for example, carbon dioxide powder manufactured by Horii Pharmaceutical Co., Ltd.) is formed in the container body. The operation of dropping and storing in a petri dish can be reliably performed.

The above-mentioned chemical for generating carbon dioxide may be introduced into a petri dish inside the container by removing the closed lid. However, in order to minimize the leakage of the generated carbon dioxide, the lid of the container body is required. The body may be provided with a through-hole through which carbon dioxide powder is charged into a petri dish in the container body, and a plug may be removably attached to the through-hole. Further, the means for adjusting the inside of the container body to a predetermined carbon dioxide gas concentration (5% concentration) is not limited to a mode in which a chemical (carbon dioxide gas powder) is reacted with water in a petri dish to generate the same. It may be directly injected from a carbon dioxide gas cylinder or the like, or a valve for the injection may be attached.

[0009]

Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a perspective view showing a simple incubator according to the present invention. In the figure, A is a small portable container main body formed of a synthetic resin material, and accommodates a petri dish B containing an egg or an embryo together with a preservative solution. A container 1 having a certain depth and a lid 2 provided with a sealable seal mechanism for closing an upper opening of the container 1.
The lid 2 is provided with latch means 3 for latching and holding the lid 2 in a sealed state. The lid 2 constituting the container body A is transparent or translucent so that the inside can be seen through, and a chemical for generating carbon dioxide gas (for example, Horii Pharmaceutical Co., Ltd.) is provided at an appropriate position on the lid 2. A through hole 4 for introducing a carbon dioxide gas powder (made by a company) into a petri dish B ′ containing water in the container body is opened, and the through hole 4 is configured to be openable and closable by a removable plug 5.

A valve 6 for connecting a base 8 at the end of a hose connected to a suction device C for making the inside of the sealed container body A a predetermined negative pressure is provided on the peripheral wall of the container 1 in the container body A. And a valve for connecting a hose base 9 connected to a carbon dioxide gas cylinder (not shown) for directly injecting carbon dioxide into the container so as to inject a carbon dioxide gas into the container into a gaseous phase having a predetermined carbon dioxide concentration. 7 is attached.

Each of the valves 6 and 7 has a check valve. The check valves are opened when a hose of a suction device or a hose of a carbon dioxide gas cylinder is connected, and the check is performed by removing the connected hose. The valve closes well known today. Note that the valves 6 and 7 may be valves provided with cocks for manual switching. In short, it is only necessary that the inside of the container body can be adjusted and maintained at a predetermined gas phase (pressure and carbon dioxide concentration).

Next, the use of the above-described incubator will be described. 2 to 4 show an embodiment in which a chemical is used as a means for adjusting the inside of the container body A to a predetermined carbon dioxide concentration.
As shown in the figure, a Petri dish B containing the eggs taken out of the ovaries of the livestock together with the preservative solution and a Petri dish B 'containing water are accommodated in the container body A, and the Petri dish B' containing water is covered with the lid 2. Is located below the through-hole 4 opened in the container D, and a chemical D that generates carbon dioxide gas from the through-hole 4 into the petri dish B ′ (for example,
Carbon dioxide powder manufactured by Horii Pharmaceutical Co., Ltd.).

After the introduction of the chemical D into the petri dish B ', the through holes 4
Is closed with the stopper 5 to generate carbon dioxide gas in the container body A. The carbon dioxide is generated by reacting the water contained in the petri dish B 'with the chemical D to generate carbon dioxide. The dosage of the chemical D is, for example, 5 ml of water in a Petri dish B 'and 0.5 to 1.0 g of carbon dioxide powder. And FIG.
As shown in (1), a suction device C is connected to the valve 6 via a hose mouthpiece 8 in order to make the inside of the container body A a predetermined negative pressure, and the suction device C is operated to make the inside of the container body A about 20 ml. .
By making the inside of the container body A negative pressure with a suction device, the container body A
The oxygen content in the cell drops from 21% to 15%, and this oxygen content and the degree of tension caused by temperature (38.5 ° C.) and negative pressure increase the incidence of embryo development. Then, when the petri dish B is taken out under the above conditions, the negative pressure is released by injecting air into the container body, and the lid 2 is set in a removable state. As a method for adjusting the inside of the container body to normal pressure, a check valve of the valve is opened by connecting a hose to the valve 7 or the like, and the container is communicated with the atmosphere.

FIGS. 5 and 6 show an embodiment in which carbon dioxide gas is directly injected into the container as a means for bringing the inside of the container body A to a predetermined carbon dioxide concentration. In this case, the valve 7 provided in the container body A is supplied with carbon dioxide gas. Connect the cylinder and inject. Then, after injecting the carbon dioxide gas, the valve 6 is similar to the above-described embodiment.
To a predetermined negative pressure inside the container body.

[0015]

According to the present invention, the incubator for maturation, fertilization and development of domestic eggs can provide a simple incubator which can be transported with a simple configuration while maintaining favorable conditions for embryo development. . Further, according to the configuration of the second aspect, the generation of carbon dioxide using a chemical (carbon dioxide powder) can be performed quickly and reliably with the lid kept. Furthermore, according to the configuration of the third aspect, the carbon dioxide gas can be directly injected into the container main body, so that it can be used widely.

[Brief description of the drawings]

FIG. 1 is a perspective view showing an example of a mature incubator according to the present invention.

FIG. 2 is a cross-sectional view showing a state in which carbon dioxide powder is charged into a petri dish in a container body.

FIG. 3 is a cross-sectional view showing a state in which a suction device is connected to a valve of the container main body to make a predetermined negative pressure inside the container.

FIG. 4 is a sectional view showing a state in which the inside of the container body is returned to normal pressure.

FIG. 5 is a partially cutaway plan view showing a state in which carbon dioxide gas is directly injected into the container main body and a predetermined negative pressure is applied to the inside.

FIG. 6 is a longitudinal sectional view of the same.

[Explanation of symbols]

A: Container body B, B ': Petri dish 1 ... Container 2 ... Lid 4 ... Through-hole 5 ... Plug body 6 ... Valve for connecting suction device 7 ... Valve for connecting carbon dioxide gas cylinder

Claims (3)

(57) [Claims] 1. A suction device for applying a negative pressure to a portable container body having a closed lid for accommodating a petri dish for accommodating an immature ovum taken out from an ovary of a domestic animal together with a preservation solution so as to be able to be taken in and out. attaching a valve with a check valve for connecting the vessel and the vessel body is carbonate Gasupauda over
Culturing, fertilizing, and developing incubating livestock ova, wherein a predetermined concentration of carbon dioxide gas is injected into the cultivator. 2. A through hole for introducing carbon dioxide powder into a petri dish filled with water in the container body is formed in the lid of the container body, and a stopper is detachably attached to the through hole. Maturation, fertilization, developmental incubator of livestock ova according to 1. 3. The incubator for maturation, fertilization and development of livestock eggs according to claim 1 or 2, wherein a valve for injecting carbon dioxide gas is attached to said container body.