CN109022264A - The auxiliary device of ovum survival rate can be improved - Google Patents
The auxiliary device of ovum survival rate can be improved Download PDFInfo
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- CN109022264A CN109022264A CN201810336747.2A CN201810336747A CN109022264A CN 109022264 A CN109022264 A CN 109022264A CN 201810336747 A CN201810336747 A CN 201810336747A CN 109022264 A CN109022264 A CN 109022264A
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- ovum
- survival rate
- vessel box
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- auxiliary device
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- 210000004681 ovum Anatomy 0.000 title claims abstract description 98
- 102000002322 Egg Proteins Human genes 0.000 title claims abstract description 94
- 108010000912 Egg Proteins Proteins 0.000 title claims abstract description 94
- 230000004083 survival effect Effects 0.000 title claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 238000010438 heat treatment Methods 0.000 claims abstract description 24
- 238000000605 extraction Methods 0.000 claims abstract description 10
- 239000010902 straw Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 8
- 239000012780 transparent material Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 abstract description 20
- 230000008569 process Effects 0.000 abstract description 16
- 238000005516 engineering process Methods 0.000 abstract description 10
- 230000004720 fertilization Effects 0.000 abstract description 7
- 210000001733 follicular fluid Anatomy 0.000 description 12
- 230000001850 reproductive effect Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 210000000349 chromosome Anatomy 0.000 description 4
- 208000021267 infertility disease Diseases 0.000 description 4
- 230000006378 damage Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000002159 abnormal effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 210000000287 oocyte Anatomy 0.000 description 2
- 210000002394 ovarian follicle Anatomy 0.000 description 2
- 210000001672 ovary Anatomy 0.000 description 2
- 230000035935 pregnancy Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241001439211 Almeida Species 0.000 description 1
- 208000034657 Convalescence Diseases 0.000 description 1
- 108010034145 Helminth Proteins Proteins 0.000 description 1
- 208000035752 Live birth Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 210000000625 blastula Anatomy 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002651 drug therapy Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000008011 embryonic death Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 244000000013 helminth Species 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000011810 insulating material Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000031864 metaphase Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000025090 microtubule depolymerization Effects 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 238000010837 poor prognosis Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
Abstract
The invention belongs to the field of medical instrument technology, more particularly, to a kind of auxiliary device that ovum survival rate can be improved.Including shell, it is provided with vessel box on shell, big culture dish and small culture dish are provided in vessel box, circulating water line is installed on vessel box, circulating water pipe road is provided with water pump and heating device, and vessel box is connected with ovum extraction system.Advantage is: covering takes ovum and picks up ovum overall process, takes ovum by the realization of ovum extraction system and picks up ovum, steady temperature range is in front of system, overall process, comprehensive holding ovum fertilization;Big culture dish and small culture dish are placed in vessel box always, rather than it is exposed in the environment, heat loss is less during taking ovum and picking up ovum, and makes vessel box be maintained at a stationary temperature range by heating device, to improve the survival rate of ovum.
Description
Technical field
The invention belongs to the field of medical instrument technology, more particularly, to a kind of auxiliary device that ovum survival rate can be improved.
Background technique
Treating infertile main method has drug therapy, operative treatment and assisted reproductive therapy, wherein auxiliary life
Growing is that treatment is one of the important means of infertile, has higher pregnancy rate compared to other two kinds for the treatment of methods.Supplementary reproduction skill
The main category of art includes artificial insemination AI and vitro fertilization-embryo implanting IVF-ET and its deriving technology two major classes, clinical number
It shows according to statistics, at least 20% Mr. and Mrs in infertile patients, it is necessary to fertility is just able to achieve by supplementary reproduction.Supplementary reproduction is
The Last Resort of infertile treatment does not obtain effect by conventional medicine and operative treatment, is likely to give birth to by auxiliary
Plantation technology is treated to realize.The assisted reproductive technology in China is very mature at present, and success rate can reach 60% or so, in the whole world
It stands out.
Ovum is taken in reproductive technology and picks up ovum operating process:
1, take ovum: clinician takes out the liquor folliculi containing ovum using ovum system such as Fig. 1 is taken out of her ovaries.Institute
The egg-taking tube road used is up to 110cm, outer diameter 5mm such as Fig. 2.Due to the individual difference of the ovarian follicle quantity of patient, from different patients
For the ovarian follicle liquid pipe number taken out in ovary between 2-10 pipe, every pipe liquor folliculi is about 10ml.According to the skilled journey of clinician
Difference is spent, is 60 seconds/pipe or so the time required to every pipe.Liquor folliculi is directly loadable into test tube, is placed on constant temp test tube rack such as Fig. 3,
Guarantee 37 DEG C of constant temperature.
2, pick up ovum: laboratory embryologist takes off test tube from constant temp test tube rack, gently by invisible spectro liquor folliculi
It pours into the culture dish of 10cm diameter, liquor folliculi will be laid in culture dish surface, and liquid thickness is generally 5mm.Culture dish is put
It is placed on the matched constant temperature objective table of disecting microscope such as Fig. 4, embryologist identifies ovum using 10 times of mirrors, manually controls bar
Single oocyte, is sucked suction pipe by the red rubber part of family name's suction pipe such as Fig. 5, then is moved into the small culture dish of 5cm diameter, Zhu Shouzai
Small culture dish is put into rapidly in the desktop incubator for having set 37 DEG C of constant temperature.This process because of embryologist's proficiency not
Together, at 90 seconds or so the time required to operation.
The technologies such as supplementary reproduction and cell biological engineering, to sperm, ovum, fertilized eggs, body early embryo, culture cell with
And in the treatment process of samples such as in vitro living tissue, the small helminth of work and microorganism, has the characteristics that its is common, that is, leaving work
In the case where body, it is both needed to be placed under microscope and is observed and handled in 35~37 DEG C of certain temperature range, can just make by sample
Product maintain normal physiological activity, so that obtain observation is appropriately determined result.
In the collection and identification process of assisted reproductive technology ovum, embryologist will take the liquor folliculi of ovum personnel collection gently
It pours into 10cm culture dish, liquor folliculi will be laid in culture dish surface, and liquid thickness is generally 5mm.Culture dish is placed in solution
It cuts open on the matched constant temperature objective table of microscope, identifies ovum using 10 times of mirrors.It cannot be covered on liquor folliculi during this operation
Mineral oil can not cover culture ware lid.Liquor folliculi at this time, which is equivalent to, to be exposed in external environment, and environmental factor such as temperature becomes
Change, pH change or light irradiation etc. have damage to ovum, influence the fertility of ovum and the developmental potentiality of after fertilization.
Pickering etc. finds that the ovum of people in room temperature in transfer process, and since room temperature is lower than 37 DEG C, spindle can become in ovum
It is small, or even the microtubule depolymerization of spindle is caused, finally result in spindle disappearance.Almeida etc. has studied 18 people's ovums, discovery
Have within 5 minutes at room temperature and spindle disruption occurs in 72% ovum, 56% occurs the depolymerization separation of chromosome, damages after rewarming
Wound can not be restored., it was also found that when temperature is cooled to room temperature only 5 minutes, ovum changes for the research such as Simerly, or
Spindle become smaller or spindle in micro-pipe disintegration even disappear completely, metaphase chromosome is also in disorder distribution.5 will be placed at room temperature for
Minute or 15 minutes people's ovums put back in 37 DEG C of incubators again 1 or 4 hour, less than half of ovum restore to not by
The control ovum handled that cools down is similar.Within convalescence, if there is the variation of cell cycle, it will will lead to chromosome number
It is abnormal.Since fertilization process is the process that ovum completes second meiotic division, chromosome number purpose is abnormal, can all cause non-whole
The generation of times body embryo tire.
Therefore the existing mating constant temperature objective table of microscope is for ensuring that the temperature in culture dish, avoids occurring in ovum
The a series of poor prognosis such as spindle disruption.According to the requirement of assisted reproductive technology standard criterion program, the mating constant temperature of microscope
Objective table temperature should be set as 37 DEG C, and environment temperature is generally located between 23~25 DEG C, and liquor folliculi spreads out the big training in 10cm
It supports in ware, liquid heat loss is too fast, and the temperature of actual measurement liquor folliculi can be lower than set 5~6 DEG C of temperature.If set
It sets and is higher than 37 DEG C, and it is excessively high to will lead to local temperature in culture dish.It is special according to Human in vitro Fertilization-embryo transfer laboratory operation
It is pointed out in family's guide 2016, liquor folliculi temperature exceeds 36.4~36.9 DEG C of this ranges when taking ovum, all will affect embryonic blastula shape
At rate, implantation rate and live birth rate.Temperature of people's ovum in vitro in operating process has to be maintained at a more constant model
Within enclosing, the fluctuation of temperature is reduced to the greatest extent in operation.Research also found that slight temperature raising will increase more micro-
Pipe forms spindle, but 40 DEG C of higher temperature can destroy spindle.And this high temperature is irreversible to the destruction of spindle
, it can also inhibit cell division in the research discovery cultivation temperature raising of other animals or lead to embryonic death.The discovery such as Wang
If the temperature in culture drop is maintained at 37 DEG C, the presence of spindle can be observed in more ovums.These ovums by
After essence, rate of fertilization and pregnancy rate have to be improved significantly.It can be seen that development of the stabilization of temperature to ovum when Microfertilization
It is considerable.
Assisted reproductive technology is developed so far, and is not occurred both at home and abroad constant before system, overall process, comprehensive ovum fertilization
Temperature control equipment covering takes ovum and picks up ovum overall process.Existing constant temp test tube rack and constant temperature objective table, which solve only, to be taken ovum and picks up
During ovum, portion link and local temperature control method are unable to reach system, overall process and in all directions in a stable temperature
Spend range.
Summary of the invention
Regarding the issue above, the present invention provides a kind of auxiliary is in steady temperature range before keeping ovum fertilization
The auxiliary device that ovum survival rate can be improved.
In order to achieve the above objectives, present invention employs following technical proposals: the auxiliary device of ovum survival rate originally can be improved,
Including shell, it is provided with vessel box on shell, big culture dish and small culture dish are provided in vessel box, is equipped with and follows on vessel box
Ring water lines, circulating water pipe road are provided with water pump and heating device, and vessel box is connected with ovum extraction system.
In the above-mentioned auxiliary device that ovum survival rate can be improved, the vessel box includes bottom plate and upper frame-type lid, bottom
Plate is equipped with several first positioning protrusion around big culture dish and several second positioning protrusion around small culture dish.
In the above-mentioned auxiliary device that ovum survival rate can be improved, the bottom plate is equipped with detent chimb, upper frame-type lid
Inner wall be equipped with inner raised edge, detent chimb matches with inner raised edge, and several ventholes are offered on the bottom plate.
In the above-mentioned auxiliary device that ovum survival rate can be improved, the bottom plate and upper frame-type lid are transparent material system
At.
In the above-mentioned auxiliary device that ovum survival rate can be improved, the ovum extraction system includes egg-taking tube road component
And negative pressure straw, egg-taking tube road component penetrate the vessel box, slidably connect Mobile base on the vessel box, Mobile base and negative
Press the universal connection of suction pipe.
In the above-mentioned auxiliary device that ovum survival rate can be improved, egg-taking tube road component includes egg-taking tube and negative pressure
Device, one end that egg-taking tube penetrates the vessel box is outlet end, and one end that egg-taking tube does not penetrate vessel box is liquid feeding end.
In the above-mentioned auxiliary device that ovum survival rate can be improved, it is equipped between the Mobile base and the negative pressure straw
Minitype universal joint.
In the above-mentioned auxiliary device that ovum survival rate can be improved, the circulating water line is heat-dissipating pipe, and radiate pipe dish
Song is in the bottom of vessel box.
In the above-mentioned auxiliary device that ovum survival rate can be improved, the heating device be heating tube, the one of heating tube
End is connected with the water inlet of heat-dissipating pipe, and the other end of heating tube is connected with the water outlet of heat-dissipating pipe.
In the above-mentioned auxiliary device that ovum survival rate can be improved, temperature-detecting device is provided in the vessel box.
Compared with prior art, the advantages of auxiliary device of ovum survival rate originally can be improved is: covering takes ovum and picks up
Ovum overall process takes ovum by the realization of ovum extraction system and picks up ovum, is in front of system, overall process, comprehensive holding ovum fertilization
Steady temperature range.Big culture dish and small culture dish are placed in vessel box always, rather than it is exposed in the environment, take ovum and pick up
Heat loss is less during ovum, and makes vessel box be maintained at a stationary temperature range by heating device, to mention
The survival rate of high ovum.
Detailed description of the invention
Fig. 1 is the cross-sectional view of provided by the invention auxiliary device.
Fig. 2 is the cross-sectional view of vessel box provided by the invention.
Fig. 3 is the top view of vessel box provided by the invention.
Fig. 4 is partial enlarged view provided by the invention.
Fig. 5 is the structural schematic diagram of egg-taking tube provided by the invention.
In figure, shell 1, vessel box 2, big culture dish 3, small culture dish 4, circulating water line 5, water pump 6, heating device 7, ovum
Sub- extraction system 8, bottom plate 9, upper frame-type lid 10, the first positioning protrusion 11, the second positioning protrusion 12, detent chimb 13, inner raised edge
14, venthole 15, egg-taking tube road component 16, negative pressure straw 17, Mobile base 18, egg-taking tube 19, negative pressure device 20, outlet end 21,
Liquid feeding end 22, minitype universal joint 23, heat-dissipating pipe 24, heating tube 25, temperature-detecting device 26.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
As shown in Figs. 1-5, the auxiliary device that ovum survival rate originally can be improved includes shell 1, is provided with vessel box on shell 1
2, big culture dish 3 and small culture dish 4 are provided in vessel box 2, and circulating water line 5, circulating water line 5 are installed on vessel box 2
On be provided with water pump 6 and heating device 7, vessel box 2 is connected with ovum extraction system 8.
Wherein, ovum extraction system 8 includes egg-taking tube road component 16 and negative pressure straw 17, and egg-taking tube road component 16 penetrates device
Ware box 2 slidably connects Mobile base 18, Mobile base 18 and the universal connection of negative pressure straw 17 on vessel box 2.Egg-taking tube road component 16
Including egg-taking tube 19 and negative pressure device 20, one end that egg-taking tube 19 penetrates vessel box 2 is outlet end 21, and egg-taking tube 19 does not penetrate
One end of vessel box 2 is liquid feeding end 22.Minitype universal joint 23 is equipped between Mobile base 18 and negative pressure straw 17.
Covering takes ovum and picks up ovum overall process, takes ovum by the realization of ovum extraction system 8 and picks up ovum, more specifically, passes through
Egg-taking tube 19 takes out ovum from human body, and direct negative pressure is drawn into big culture dish 3, without being put into test tube after taking ovum
Then this step identifies ovum using 10 times of mirrors, manually control the red rubber part of negative pressure straw 17, Single oocyte is inhaled
Enter negative pressure straw 17, then moves into small culture dish 4.
Big culture dish 3 and small culture dish 4 are placed on always in vessel box 2, rather than it is exposed in the environment, reduce heat
Scatter and disappear, take ovum and heat loss be less during picking up ovum, and heating device 7 heats circulating water line 5, circulating water line 5
Even heat can be passed to vessel box 2, so that vessel box 2 is maintained at a stationary temperature range, to improve ovum
Survival rate.
More specifically, vessel box 2 includes bottom plate 9 and upper frame-type lid 10, if bottom plate 9 is equipped with around big culture dish 3
Dry first positioning protrusion 11 and several second positioning protrusion 12 around small culture dish 4.First positioning protrusion 11 is in order to big
The positioning of culture dish 3 avoids big culture dish 3 from sliding in vessel box 2, and the second positioning protrusion 12 is avoided for small culture dish 4
Small culture dish 4 slides in vessel box 2.
Bottom plate 9 is equipped with detent chimb 13, and the inner wall of upper frame-type lid 10 is equipped with inner raised edge 14, detent chimb 13 and convex
Side 14 matches, and several ventholes 15 are offered on bottom plate 9.Upper frame-type lid 10 covers when on bottom plate 9, and inner raised edge 14 is resisted against card
On the chimb 13 of position, and the side wall of detent chimb 13 and the inner wall of upper frame-type lid 10 fit, upper frame-type lid 10 and upper frame-type lid 10
Fixation, venthole 15 are then to avoid the problem that anoxic in order to which inner air and outer air can exchange.
Bottom plate 9 and upper frame-type lid 10 are that transparent material is made, and transparent material is glass, be conducive to inside vessel box 2 into
Row observation.Further, it is also covered with transparent heat insulating material on bottom plate 9 and upper frame-type lid 10, improves heat insulation effect, is conducive to temperature
It is maintained at constant range.
Circulating water line 5 is heat-dissipating pipe 24, the tortuous bottom in vessel box 2 of heat-dissipating pipe 24.Heating device 7 is heating tube
25, one end of heating tube 25 is connected with the water inlet of heat-dissipating pipe 24, the other end of heating tube 25 and the water outlet of heat-dissipating pipe 24
It is connected.Temperature-detecting device 26 is provided in vessel box 2.The temperature of vessel box 2 is able to detect by temperature-detecting device 26,
When the temperature is too low, then start heating tube 25 to be heated, when the temperature is excessively high, then stop the heating of heating tube 25.Temperature inspection
Survey device 26 is thermometer.
Specific embodiment described herein is only to give an example to the present invention.The technical field of the invention
Technical staff can make various modifications or additions to the described embodiments or be substituted in a similar manner, but
Without departing from the present invention or beyond the scope of the appended claims.
Although term is used more herein, it does not exclude the possibility of using other terms.Use these terms
It is only for the convenience of describing and explaining the nature of the invention;Be construed as any additional limitation all and be with
What the present invention disagreed.
Claims (10)
1. a kind of auxiliary device that ovum survival rate can be improved, which is characterized in that including shell (1), shell is provided with device on (1)
Ware box (2), vessel box (2) is interior to be provided with big culture dish (3) and small culture dish (4), and vessel box is equipped with circulating water line on (2)
(5), water pump (6) and heating device (7), vessel box (2) and ovum extraction system (8) phase are provided on circulating water line (5)
Connection.
2. the auxiliary device according to claim 1 that ovum survival rate can be improved, which is characterized in that the vessel box (2)
Including bottom plate (9) and upper frame-type lid (10), bottom plate (9) is equipped with several first positioning protrusion (11) around big culture dish (3)
And several second positioning protrusion (12) around small culture dish (4).
3. the auxiliary device according to claim 2 that ovum survival rate can be improved, which is characterized in that on the bottom plate (9)
Equipped with detent chimb (13), the inner wall of upper frame-type lid (10) is equipped with inner raised edge (14), detent chimb (13) and inner raised edge (14)
It matches, offers several ventholes (15) on the bottom plate (9).
4. the auxiliary device according to claim 3 that ovum survival rate can be improved, which is characterized in that the bottom plate (9) and
Upper frame-type lid (10) is that transparent material is made.
5. the auxiliary device according to claim 2 that ovum survival rate can be improved, which is characterized in that the ovum draws system
System (8) includes egg-taking tube road component (16) and negative pressure straw (17), and egg-taking tube road component (16) penetrates the vessel box (2), institute
It states and is slidably connected on vessel box (2) Mobile base (18), Mobile base (18) and negative pressure straw (17) universal connection.
6. the auxiliary device according to claim 5 that ovum survival rate can be improved, which is characterized in that egg-taking tube road group
Part (16) includes egg-taking tube (19) and negative pressure device (20), and one end that egg-taking tube (19) penetrates the vessel box (2) is outlet end
(21), it is liquid feeding end (22) that egg-taking tube (19), which does not penetrate one end of vessel box (2),.
7. the auxiliary device according to claim 5 that ovum survival rate can be improved, which is characterized in that the Mobile base (18)
Minitype universal joint (23) are equipped between the negative pressure straw (17).
8. the auxiliary device of ovum survival rate can be improved described according to claim 1 or 2 or 3 or 4 or 5, which is characterized in that institute
Stating circulating water line (5) is heat-dissipating pipe (24), the tortuous bottom in vessel box (2) of heat-dissipating pipe (24).
9. the auxiliary device according to claim 8 that ovum survival rate can be improved, which is characterized in that the heating device
It (7) is heating tube (25) that one end of heating tube (25) is connected with the water inlet of heat-dissipating pipe (24), the other end of heating tube (25)
It is connected with the water outlet of heat-dissipating pipe (24).
10. the auxiliary device of ovum survival rate can be improved described according to claim 1 or 2 or 3 or 4 or 5, which is characterized in that
Temperature-detecting device (26) are provided in the vessel box (2).
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN112273293A (en) * | 2020-10-25 | 2021-01-29 | 马博 | Ovum collecting device with supplementary structure of distinguishing |
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CN104115782A (en) * | 2013-04-23 | 2014-10-29 | 旌德县鸿雁养殖有限公司 | Incubation method for improving yolk absorption rate of wild goose eggs |
CN104403944A (en) * | 2014-11-06 | 2015-03-11 | 徐小杨 | Automatic device and method for egg cell in-vitro fertilization and cleavage culture |
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