JP3028280B2 - Propolis-containing dispersion composition and method for producing the same - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

FIELD OF THE INVENTION The present invention relates to a stable dispersion in oil containing bee-produced propolis.

[0002]

2. Description of the Related Art Propolis is a substance produced by a worker bee that collects sap such as young shoots of a plant and mixes it with its own saliva, and contains resin components, aromatic oil, wax, volatile oil, pollen, minerals, and the like. Substance. This propolis has a function as a defense fence to prevent invasion of foreign enemies into the beehive, as well as a function as a reinforcing agent when closing the crack in the nest,
It is known that it is also used to cover corpses such as small animals and insects to prevent decay. Propolis having such various functions has antibacterial action even in humans,
It has been recognized that it has a wide range of physiological activities such as antiviral action, macrophage activation, and antiulcer action, and the use of propolis in the treatment or prevention of diseases such as infectious diseases and ulcers has been proposed (Toshiyuki Sugimoto) , Food Industry, 199
3 , page 65). Normally, propolis is collected as a dark green-brown resinous material used by worker bees to close cracks in hive boxes, and in Japan, Brazil, Australia,
Those originating from New Zealand, China, etc. are available. The original mass is evaluated based on the flavonoid content and the like, but the content of the component varies depending on the production area. In terms of quality, those from Brazil, especially from the State of Minas, are mainly made of eucalyptus sap and are considered to be of high quality.

[0003] Propolis uses the original mass as it is
But not alcoholic, such as 90-99.5% ethanol
Used as a solution (tincture). Propolis tincture
To manufacture, propolis raw mass is ground or crushed to an appropriate size.
Immersed in 90-99.5% ethanol for a few months
Thereafter, a method of removing solid matter is employed. Manufacturing in a short time
To make a small piece of raw mass, add 90 to 99.5% ethanol
Medium, with stirring at a temperature of 40-60 ° C for 6-24 hours to extract
After cooling, further cooling to remove the wax component
Alternatively, water may be added to remove insoluble components.
Provolis tincture is used on soils, stings
It has a strong odor and cannot be taken as it is.
I always take a few drops in water.

[0004]

Conventionally, propolis tincture has been
Since there is no effective method of ingestion other than taking the drug in water, it is necessary to use a container with a lid attached to the lid, which is troublesome to handle. In addition, even if the propolis is dispersed in water in a container such as a cup, the original irritating taste and odor still remain, and cannot be easily taken.
It was difficult for old people and children. In addition, there is a problem that wax adheres to the inner wall of the cup or odor remains, and it is difficult to remove the cup by washing. Therefore, anywhere
The development of propolis products that can easily consume propolis has been awaited. It is thought that this problem can be solved by encapsulating propolis in soft gelatin capsules or the like.However, it is not possible to form a capsule having a normal shape due to the influence of alcohol or water in the tincture. Because of the volatilization of water and the inability to maintain its shape, soft encapsulation of propolis tincture was not possible.

[0005]

Means for Solving the Problems The present invention has been made to solve the above problems, there is provided an oil dispersion compositions of the charging Ten possible propolis soft capsule. The composition of the present invention has a residual ethanol of 1% or less,
Propolis alcohol extraction component (hereinafter simply referred to as propolis
It may be referred to) 5 to 80% by weight, fat 0.1 to 50
% By weight, 0.1 to 50% by weight of an emulsifier, and 0.1% of a dispersion stabilizer.
1 to 10% by weight . In the compositions of the present invention, propolis is the active ingredient in the tincture is in a state of being uniformly dispersed in the dispersion medium, which can be held stably by <br/> charge TEN soft capsule.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION The fats and oils used in the composition of the present invention are as follows:
It can be arbitrarily selected from fats and oils used for food and pharmaceuticals. Animal fats, vegetable fats, fish oils and the like can be generally used. Examples of animal oils and fats include beef tallow, lard, chicken oil and the like, vegetable oils and oils include soybean oil, safflower oil, rapeseed oil, olive oil and the like, and fish oils include sardine oil and tuna oil. In addition, oils that are not glycerides such as squalene can also be used. It is desirable that these fats and oils do not dissolve the propolis and are low-viscosity liquids that can be filled in soft capsules in a dispersed state.Preferred fats and oils are vegetable oils, for example, soybean white oil, egoma Oil, safflower oil and the like.

As emulsifiers, fatty acid monoglycerides,
Fatty acid sorbitan esters, fatty acid sugar esters,
Fatty acid polyglycerin esters, lecithin, egg yolk oil and the like can be mentioned. Egg yolk oil, sugar esters of stearic acid or polyglycerol esters are preferred. The composition of the present invention may use one or more emulsifiers in combination. Examples of dispersion stabilizers include casein, albumin, pectin, guar gum, sodium CMC, and beeswax, with beeswax being most preferred.

In the composition of the present invention, the ratio of propolis, oil and fat, emulsifier and dispersion stabilizer in which propolis is stably dispersed is 10 to 50% by weight of a propolis alcohol extract component and 5 to 50% by weight of oil and fat. %, Emulsifier 5-5
0% by weight and 0.1 to 5% by weight of a dispersion stabilizer, preferably 20 to 50% by weight of a propolis alcohol extract, 10 to 35% by weight of an oil or fat, and 20 to 50% by weight of an emulsifier.
And 0.5 to 3% by weight of the dispersion stabilizer. As specific examples of the dispersion composition of the present invention, propolis alcohol extract component 20%, soybean white squeezed oil 39%, extracted egg yolk oil (phospholipid content 25%) 39%, polyglycerin stearate (HLB11.5) 1% , Dense wax 1
% Composition or propolis alcohol extract 1
3%, perilla oil 32%, extracted egg yolk oil (phospholipid content 30
%) 52% and stearic acid sugar ester (HLB)
11.0) 3%.

In order to produce the composition of the present invention, first, alcohol and water are removed from the propolis tincture, and the mixture is dispersed in the above-mentioned dispersion medium comprising fats and oils, an emulsifier and a stabilizer. However, it is difficult to obtain a stable dispersion because the powder particles are coarse even when dispersed. However, according to the following method of the present invention, efficient production can be achieved.

Propolis tincture (propolis content 2.
0.001 to 5 parts by weight, preferably 0.001 to 2 parts by weight, 0.001 to 2 parts by weight of emulsifier,
1 to 5 parts by weight, preferably 0.001 to 2 parts by weight, more preferably 0.01 to 1 part by weight, dispersion stabilizer 0.001
To 0.5 parts by weight, preferably 0.03 to 0.3 parts by weight. Two or more emulsifiers may be used in combination. However, when using egg yolk oil as the emulsifier, it is preferable to increase the blending ratio, and 0.1 to 1.5 parts by weight per 1 part by weight of propolis tincture, Preferably 0.5 to 1.5
Use parts by weight. When using egg yolk oil with other emulsifiers,
Other emulsifiers may be used in the range of 0.005 to 0.05 parts by weight.

When a mixture containing propolis tincture, oil and fat, emulsifier and stabilizer in the above ratio is stirred at 10 to 50 ° C., preferably 20 to 40 ° C. for 1 to 5 hours, a uniform dispersion can be obtained. When the fats and oils are not completely liquid at normal temperature, it is desirable to heat to 30 to 40 ° C. Mixing is
Stirring is performed in a mixing tank equipped with a usual impeller stirrer, or using a mixer such as a homogenizer. Then, by removing the alcohol and, if desired, water according to a conventional method, the composition of the present invention as defined above, in which propolis is uniformly dispersed, can be obtained. The alcohol may be removed under normal pressure and under heating, but about 1 to prevent the change of the active ingredient.
It is preferable to heat and distill under reduced pressure of not more than 00 mmHg by using a rotary evaporator, a centrifugal molecular distillation apparatus or the like while stirring as required.

The thus-obtained dispersion of propolis in oil can be used as it is for the purpose of treatment or the like, but if it is filled into soft gelatin capsules according to the usual method of formulation, it is easy to take and easy to handle. This is advantageous in terms of stability and stability of components. Soft encapsulation can be performed by methods known in the art, and the rotary die method is most common, but may be a flat plate type or a seamless type. The capsule shell is commonly used in the fields of pharmaceuticals and food processing, and is not particularly limited as long as it can maintain a stable shape when filled with the composition of the present invention. , Glycerin, sorbitol and the like are suitable. The filling amount of the composition of the present invention into the soft capsule is usually in the range of 100 mg to 1000 mg / capsule, preferably 2 mg / capsule.
The range is 50-300 mg / capsule. The shape of the capsule is also arbitrary, and may be an oval type, a Suppo type, a triangle, or the like.

[0013]

The present invention will be described in more detail with reference to the following examples, which should not be construed as limiting the invention. Example 1 Commercially available propolis tincture (solid content 25%, 95% ethanol solution) 10 kg, soybean white squeezed oil 5 kg, extracted egg yolk oil (phospholipid content 25%) 5 kg and polyglycerin stearate (HLB 11.5) 0.1 kg .
1 kg is charged into a mixer equipped with stirring blades, and mixed by stirring for 2 hours. The mixture is charged into a rotary evaporator, and ethanol is distilled off at 60 ° C. under a reduced pressure of 20 mmHg. When the distilled ethanol is no longer observed, it is taken out to obtain a uniform dispersion. Residual ethanol was 1% or less.

Example 2 10 kg of commercial propolis tincture (solid content 25%, 95% ethanol solution), perilla oil 6 kg, extracted egg yolk oil (phospholipid content 30%) 10 kg, stearic acid sugar ester (HLB 11.0) 0.5 kg Into a mixer equipped with stirring blades, and stirred for 2 hours to mix. The mixture is charged into a centrifugal molecular distillation apparatus, and ethanol is distilled off under reduced pressure of 70 ° C. and 10 ⁻³ mmHg. After circulating four times, remove. Residual ethanol was 10 ppm or less. Since partial separation was observed, the mixture was dispersed again with a homogenizer to obtain a uniform dispersion.

Example 3 Preparation of Soft Capsules The dispersion composition obtained in Example 1, the dispersion composition obtained in Example 2, and commercially available propolis tincture (solid content: 25%, 95%
% Ethanol solution), respectively. 5
A soft capsule was prepared. The rind is made by adding glycerin to gelatin.
The capsules filled with 0 mg were checked for encapsulation, and the capsules were allowed to stand at 30 ° C. for 6 months to check the contents. The results are as follows.

【table】 1): No capsule was formed, and gelatin of the outer skin was dissolved. 2): The appearance of the capsule was unchanged, and the dispersed state of the contents was stable.

[0016]

According to the present invention, since propolis, which was conventionally available only in the form of an alcoholic solution, is provided in the form of a stable oil-dispersed composition, handling becomes extremely easy. Further, by preparing the composition of the present invention into a soft capsule, propolis having excellent physiological activity can be widely used for promoting human health or treating diseases.

────────────────────────────────────────────────── ─── Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI B01F 17/34 B01F 17/34 17/56 17/56 (56) References JP-A-6-70706 (JP, A) JP Hei 7-75534 (JP, A) JP-A-61-197523 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) A23L 1/05-1/09 A61K 35/64 B01F 17 / 00 B01F 17/14 B01F 17/30 B01F 17/34 B01F 17/56

Claims (9)

(57) [Claims] 1. The method according to claim 1, wherein the residual ethanol is 1% or less, 5 to 80% by weight of a propolis alcohol extract component, and 0.
A dispersion composition in oil comprising 1 to 50% by weight, 0.1 to 50% by weight of an emulsifier and 0.1 to 10% by weight of a dispersion stabilizer. 2. The composition according to claim 1, wherein the fat is selected from animal fat, vegetable fat or fish oil. 3. The composition according to claim 1, wherein the emulsifier is selected from fatty acid monoglyceride, fatty acid sorbitan ester, fatty acid sugar ester, fatty acid polyglycerin ester, lecithin and egg yolk oil. 4. The composition according to claim 1, wherein the dispersion stabilizer is selected from casein, albumin, pectin, guar gum, sodium CMC, and beeswax. 5. The composition according to claim 1, which is filled in a soft capsule. 6. An oil or fat containing 0.001 to 5 parts by weight per part by weight of an alcohol solution containing 2.5 to 40% of propolis.
Parts, an emulsifier 0.001 parts by weight and the dispersion stabilizer 0.001 parts by weight, then and removing the alcohol, residual ethanol of 1% or
Below, in an oil containing 5 to 80% by weight of a propolis alcohol extract component, 0.1 to 50% by weight of fat and oil, 0.1 to 50% by weight of an emulsifier and 0.1 to 10% by weight of a dispersion stabilizer A method for producing a dispersion composition. 7. The method according to claim 6, wherein the fat is selected from animal fat, vegetable fat or fish oil. 8. The method according to claim 6, wherein the emulsifier is selected from fatty acid monoglyceride, fatty acid sorbitan ester, fatty acid sugar ester, fatty acid polyglycerin ester, lecithin and egg yolk oil. 9. The method according to claim 6, wherein the dispersion stabilizer is selected from casein, albumin, pectin, guar gum, sodium CMC, and beeswax.