JP2931744B2 - Method and apparatus for compensating for twist in experimental animal leads, tubes, optical fibers, etc. - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for compensating twisting of experimental animal lead wires, tubes, optical fibers, etc., which enables collection of data during breeding of experimental animals in a cage in a free state for a long time without any trouble. Regarding the device.

[0002]

2. Description of the Related Art Conventionally, slip rings have been used for compensating for torsion of a tube for measuring a myocardium (electrocardiogram) and an electroencephalogram of an experimental animal, and for injecting a drug solution into the experimental animal and collecting blood and body fluid. There is a sebel.

[0003] Slip rings using a rotating electrode for transmitting an electric signal and a current collecting shoe have satisfactory performance, but a fluorocarbon resin is used for a rotary seal of a tube for a sebel for a chemical solution or the like. There is only a seal type.

It is well known that an optical fiber is used to obtain an optical signal of a living body at an arbitrary site in an experimental animal. However, there is no suitable optical fiber for compensating the twist of the optical fiber.

[0005]

The conventional seal type sebel rotates with the force of an animal, so that it cannot be tightly fitted, leaks liquid, and causes a liquid mixture. Difficult, limited to 4 channels.

Further, since the seal is formed concentrically, a dead volume which is inconvenient for accurate microinjection and body fluid collection is generated for each channel, the volume increases, occupies extra space, and observes experimental animals. There are drawbacks such as obstructing the visibility at the time.

Further, a seal of a sebel using a fluororesin has a life and has a drawback that maintenance is required.

In order to obtain an optical signal of a living body at an arbitrary site in an experimental animal, the twist of the optical fiber cannot be compensated.
There was a drawback that this kind of experiment was restricted.

According to the present invention, a highly reliable torsion compensation for a laboratory animal lead wire, a tube, an optical fiber, etc., which does not use a conventional sebel, can be multi-channeled, has no dead volume, and can compensate for optical fiber torsion. It is an object to provide a method and its device.

[0010]

Means for Solving the Problems A circular floor is made rotatable with a roller on a base, and an open bottom of a cage for breeding experimental animals is arranged on the floor, and the cage is fixed on the base. Then, a hole is made in the center of the top surface of the cage.

The hole is provided with a tube or an optical fiber connected to the experimental animal, or a rotating shaft having a through-hole through which the lead wire and the tube are inserted, and a slip ring for compensating the twist of the lead wire by the movement of the experimental animal. When,
A sensing device for sensing the amount and direction of twisting of the tube or optical fiber or for sensing the amount and direction of twisting of the lead wire and the tube is provided above the hole.

Alternatively, only a similar detector is provided which senses the amount and direction of torsion between the lead wire and the tube due to the movement of the experimental animal.

Further, a motor is provided on the outer periphery of the circular floor to receive a signal from the detection device, and a rotation drive unit is provided between the motor and the outer periphery of the floor for rotating the motor in a direction opposite to the twisting direction.

[0014]

The outer peripheral portion 4b of the circular floor surface 4 having the hole 4a in the shape of a sword is supported by the frame 5 by four rollers 2 rotatably supported by the shaft 3 on the base 1. The floor 4 is rotatable by guiding the frame 5 with the respective rollers 2.

Then, the bottom of a cylindrical cage 6 for breeding laboratory animals having an open bottom is placed on the floor 4 and the cage 6
The four mounting brackets 8 fixed to the outer periphery at the lower end are inserted into the male screws 3a at the ends of the shafts 3 of the respective rollers 2, so that the cage 6 can be fixed by inserting the mounting screws 8a into the shafts 3.

A hole 7 is provided at the center of the upper surface of the cage 6,
Equipped with a tube 10 or an optical fiber 10a connected to the experimental animal above the hole 7 or a rotating shaft 12a having a lead wire 9 and a through hole 11 through which the tube 10 is inserted, and detects the amount and direction of the twist. An apparatus 12 is provided.

At this time, when inserting only the tube 10 or the optical fiber 10a into the through hole 11 of the rotating shaft 12a, the lead wire 9 is connected to a slip ring 13 fixed to the lower end of the detecting device 12, or When the lead wire 9 is not connected to the slip ring 13 and only the lead wire 9 is connected to the slip ring 13, the tube 10 may not be inserted into the through hole 11. It is not necessary to provide the slip ring 13.

A motor 14 is provided for rotating upon input of a signal from the detection device 12, and an outer peripheral portion 4b of the floor 4 is provided.
Is rotated in a direction opposite to the twisting direction via the rotation drive unit 15.

Therefore, only the lead wire 9 twisted by the rotational movement of the experimental animal 27 is compensated for twist by the slip ring 13, and the amount and direction of the twist between the tube 10 or the optical fiber 10 a or the lead wire 9 and the tube 10 are detected by the detector 12. Upon sensing, motors 14 rotate floors 4 in opposite directions of their twist.

That is, when the lead wire 9, the tube 10, and the optical fiber 10a are twisted by the movement of the experimental animal 27,
The twist of the lead wire 9 is compensated by the slip ring 13, the twist of the tube 10 or the optical fiber 10 a or the twist of the lead wire 9 and the tube 10 is detected by the detecting device 12, and the motor 14 rotates the floor 4 in the opposite direction of the twist. Therefore, those twists are compensated by the rotation of the floor 4.

Further, since the twist of the optical fiber 10a can be compensated, the optical fiber 10a can be connected to the experimental animal 27, and it is possible to obtain an optical signal of a living body at an arbitrary part of the experimental animal 27.

[0022]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Laboratory animal leads used in the practice of the present invention,
The accompanying drawings showing an example of a torsion compensator such as a tube and an optical fiber will be described in detail.

FIG. 1 is an overall perspective view, FIG. 2 is a plan view of the same, FIG. 3 is a view of a floor, FIG. 4 shows a rotation drive unit, and FIG. 5 is a view of a rotation detection device and a slip ring. Things.

FIG. 6 shows an example in which a lead wire for transmitting an electric signal, a tube for flowing a chemical solution, and an optical fiber for transmitting an optical signal are inserted into a slip ring and a rotation detecting device.

FIG. 7 shows a rotation detecting device, and FIG. 8 is a wiring diagram showing a connection state between the rotation detecting device and the motor.

Reference numeral 1 denotes a base, in which a through hole 1a having the same size as the bottom of a cage 6 to be described later is formed at the center, and is made by processing a stainless steel plate into a square base.

Reference numeral 2 denotes a plastic grooved roller, which is rotatably supported on the four corners of the base 1 by bearings 3 via bearings, and the base end of each shaft 3 is attached to the base 1 as shown in FIG. It is fixed as shown, and has a male screw 3a cut at the tip.

Reference numeral 4 denotes a floor surface having the same size as the bottom of the cage 6 to be described later. A through-hole 4a is formed in the center, and a stainless steel bar 29 is formed in the hole 4a in the shape of a cage. 4
b.

Reference numeral 5 denotes a frame body having a stepped hole 5a for inserting and supporting an outer peripheral portion 4b of the floor surface 4. The outer periphery of the frame body 5 is formed by grooves 2a of the four grooved rollers 2. And the floor surface 4 is made rotatable.

Reference numeral 6 denotes a cylindrical cage for freely raising the experimental animal 27 for an arbitrary period of time, for example, one week or more, and is made of a transparent acrylic resin or the like with an open top and a closed bottom. Then, a hole 7 is provided in the center of the upper surface.

Reference numeral 8 denotes an L-shaped mounting bracket fixed to the four lower portions of the cylindrical cage 6. The lower side protrudes in the outer circumferential direction of the cage 6, and a bolt hole 8 b for inserting the screw 3 a of the shaft 3 is provided. At 8a, it is fixed to the tip of the shaft 3.

Reference numeral 9 denotes a lead wire for measuring a muscle (cardiogram), an electroencephalogram, and the like of the experimental animal 27, 10 denotes a tube for injecting a drug solution into the experimental animal 27, and collects blood and body fluid, and 10a denotes an experimental animal. 27 are optical fibers for transmitting optical signals of specific portions, each of which has one end connected to an experimental animal 27.

Reference numeral 12 denotes a tube 10 or an optical fiber 10
a or a rotation detecting device as a detecting device for detecting the amount and direction of the twist between the lead wire 9 and the tube 10;
Photosensors 17, 17 provided with an infrared light emitting diode light emitter and a phototransistor light receiver opposed to each other above and below a meniscus 16 fixed to 2a, are rotated by 90 ° so as to generate two-phase electric signals of A and B. Place at an angle.

Then, the two-phase electric signals are input to the microcomputer 18, and the direction of rotation (torsion) and the number of rotations (the amount of twist) are detected by software of the computer 18.

A tube 10 or an optical fiber 10a or a lead wire 9 from an experimental animal 27 is attached to the rotating shaft 12a.
And a through hole 11 through which the tube 10 is inserted.

Then, the rotating shaft 12a is rotated together with the tube 10 or the optical fiber 10a or the lead wire 9 and the tube 10 which are twisted by the rotational movement of the experimental animal 27, and the direction and amount of the twist are detected.

Numeral 13 denotes a slip ring, which is a rotation detecting device 1
2, a current collecting shoe mounting base 19 is fixed to a lower end, and a protrusion 20 is provided at a lower end of the mounting base 19;
A hollow rotating electrode mounting shaft 21 is fixed to the lower end of the second rotating shaft 12a.

The noble metal ring 22 as a rotating electrode
Are inserted into the rotating electrode mounting shaft 21 by the required number of lead wires 9, and the mounting shaft 21 and each ring 22 are insulated and fixed.

Reference numeral 23 denotes a current collecting shoe for transmitting an electric signal by contacting the tip with each of the noble metal rings 22. The current collecting shoe is attached to a current collecting shoe mounting portion 24 on one surface of the projecting portion 20, and an electroencephalogram, a muscle (heart) ) Attach the lead wire 9 to the recorder 25 such as an electrogram.

Then, the lead wire 9 connected to the experimental animal (rat) 27 is attached to the lead wire take-out portion 26 connected to the outer surface of the rotating electrode mounting shaft 21 and connected to each noble metal ring 22.

Finally, the cylindrical slip ring cover 28 is inserted into the current collecting shoe mounting base 19, fixed with screws 28a, and a lead wire from the upper end of the cover 28 to the recorder 25 connected to the base of the current collecting shoe 23. 9 is provided with a lead wire extraction groove 28b.

Reference numeral 14 denotes a motor composed of a stepping motor or a servo motor, which receives a pulse from the rotation detecting device 12 and rotates through a motor driver circuit 29 in the torsion direction by the number of pulses proportional to the amount of torsion.

Numeral 15 denotes a rotary drive unit, which comprises a rubber roller inserted into a rotary shaft 14a of a motor 14 through a spacer 15a, and an outer peripheral portion of a frame 5 into which an outer peripheral portion 4b of the floor 4 of the cage 6 is inserted. Be attached to

The rotation drive unit 15 is driven by the motor 14.
Is rotated by the number of pulses proportional to the amount of torsion, and the floor surface 4 is turned into a tube 10 or an optical fiber 10 a or a lead wire 9.
And the tube 10 is rotated by the amount of twist in the direction opposite to the twist.

Reference numeral 30 denotes a rotation detecting device mounting base, and a mounting plate 31 on which the detecting device 12 is mounted is mounted on a screw 3 with a handle.
2. Rotatably fix with 2.

Then, columns 33, 33 standing on the upper surface of the cage 6 are inserted into the through holes 30a, 30a on both sides, and are fixed from both ends by screws 32, 32 with handles so as to be movable up and down.

Reference numeral 34 denotes an arm for easily rotating the rotating shaft 12a of the rotation detecting device 12 by the experimental animal 27.
The hollow rotary electrode mounting shaft 21 fixed to the lower end of the rotary shaft 12a is inserted and fixed to the lower end.

The lead wire 9 via the slip ring 13 and the through hole 11 of the rotating shaft 12a of the rotation detecting device 12
And tube 1 inserted through hollow rotating electrode mounting shaft 21
The optical fiber 10a or the optical fiber 10a or the lead wire 9 and the tube 10 are connected to the experimental animal 27. At this time, only the lead wire 9 is tied to the arm 34 with the thread 35, and the tube 10 or the optical fiber 10a is Attach it to tape 9 or the like.

Reference numeral 36 denotes a device for injecting and recovering a drug solution, blood, etc.
A device for injecting and collecting with a syringe installed in a pump, a chemical tank and an analytical instrument (both not shown)
Connect to

Reference numeral 37 denotes an experimental animal 2 attached to the outer periphery of the cage 6.
7 is a known feeding device, in which granular feed and medicine are given, and the feed amount is recorded by an infrared sensor.

Reference numeral 38 denotes a similar water supply device. When the experimental animal 27 puts a mouth on the water supply port, 50 μl of liquid droplets are supplied, and the amount of water supply is recorded by an infrared sensor.

Reference numeral 39 denotes an optical signal processing device which processes optical data obtained by using light to enable monitoring and measurement of blood flow, images, etc., and which is connected to the other end of an optical fiber. .

In using this, an experimental animal 27 such as a rat to which the necessary lead wire 9, tube 10, and optical fiber 10a are connected is accommodated in the cage 6, and the lead wire 9 is tied to the arm 34 with a thread 35. The tube 10 and the optical fiber 10 a are attached to the lead wire 9, and the respective tips are taken out above the hole 7 at the upper end of the cage 6.

FIG. 6 shows a tube 10 and an optical fiber 10.
Although a combination of "a" and "a" is shown, such a combination may be used, or each of them may be used alone.

Next, the lead wire 9 is connected to the slip ring 13.
Then, the tube 10 or the optical fiber 10a (or the lead wire 9 and the tube 10) is passed through the through-hole 11 of the rotation detecting device 12, and a recorder 25 for electroencephalogram, electromyogram, etc., a device for injecting and recovering a drug solution 36, an optical signal, respectively. It is connected to the processing device 39.

When the experimental animal 27 rotates, the twist of the lead wire 9 is compensated by the slip ring 13.

On the other hand, the tube 10 or the optical fiber 10
The torsion of the a (or the lead wire 9 and the tube 10) is detected by the rotation detecting device 12 in the amount and direction of the torsion, and the floor 4 is rotated in the direction opposite to the torsion direction by the rotation drive unit 15 via the motor 14. Compensation.

[0058]

According to the present invention having the above-described structure, it is not necessary to use a conventional sebel, and it is possible to increase the number of channels of the drug solution tube.

For example, a specific site in the brain is left and right bilateral so that one tube for supplying a chemical solution (two channels in total), one tube for sampling a substance in the brain (two channels in total), a vein, A complicated combination such as one (one channel) for a total of five channels for injecting an internal chemical solution is possible.

Since the present invention does not use a sebel, the measuring instrument and the experimental animal 27 can be directly connected, there is no dead volume, no extra space is occupied, and the visibility is improved.

Further, since the connection is made directly, there is no need to worry about liquid leakage and the like, and the reliability of the experiment can be improved.

Furthermore, it is possible to monitor and measure an optical signal at a specific site of the experimental animal 27, which has been impossible so far, so that the accuracy of the animal experiment can be further improved and its reliability can be improved. .

[Brief description of the drawings]

FIG. 1 is a perspective view showing the entirety of a torsion compensator for a laboratory animal lead wire, a tube, an optical fiber and the like of the present invention.

FIG. 2 is a plan view of the torsion compensator.

FIG. 3 is a perspective view of a floor surface of the torsion compensator with a cage removed.

FIG. 4 is an enlarged side view of a part of the rotation drive unit of the torsion compensator, showing the rotation drive unit in section;

FIG. 5 is a perspective view of a rotation detecting device and a slip ring showing an exploded state of a slip ring portion of the torsion compensating device.

FIG. 6 is a schematic explanatory diagram showing an example of a state of wiring and piping to the torsion compensator.

FIG. 7 is an exploded perspective view schematically showing a rotation detecting device of the torsion compensating device.

FIG. 8 is a block diagram showing wiring of a rotation detection device and a motor of the torsion compensator.

[Explanation of symbols]

 Reference Signs List 1 base 1a through hole 2 grooved roller 2a groove 3 shaft 3a male screw 4 floor surface 4a through hole 4b outer peripheral part 5 frame 5a stepped hole 6 cage 7 hole 8 mounting bracket 8a mounting screw 8b bolt hole 9 lead wire 10 tube DESCRIPTION OF SYMBOLS 10a Optical fiber 11 Through-hole 12 Rotation detection device 12a Rotation axis 13 Slip ring 14 Motor 14a Rotation axis 15 Rotation drive part 15a Spacer 16 Meniscus 17 Photosensor 18 Microcomputer 19 Current collecting shoe mounting base 20 Projection 21 Rotation electrode mounting axis 22 Noble metal ring 23 Current collecting shoe 24 Current collecting shoe mounting part 25 Recorder for electroencephalogram, electromyogram, etc. 26 Lead wire taking out part 27 Experimental animal 28 Slip ring cover 28a Screw 28b Lead taking out groove 29 Stainless steel rod 30 Rotation detection Equipment mounting base 30a Through-hole 31 Mounting plate 32 Screw with handle 33 Post 34 Arm 35 Thread 36 Injection and recovery device 37 Feeding device 38 Water supply device 39 Optical signal processing device

Continuation of the front page (56) References JP-A-62-222105 (JP, A) JP-A-62-97964 (JP, A) Actually open JP-A-62-57673 (JP, U) (58) Fields investigated (Int) .Cl. ⁶ , DB name) A01K 67/00 A61D 7/00 G01B 11/26

Claims (9)

(57) [Claims] 1. Detecting torsion of a tube connected to an experimental animal, detecting the amount and direction of the torsion, and rotating the cage floor for breeding the experimental animal in the opposite direction to the torsion direction to compensate for the torsion of the tube. A method for compensating for a torsion of a tube for a laboratory animal. 2. The twist of an optical fiber connected to a laboratory animal is sensed, the amount and direction of the twist is detected, and the cage floor for breeding the laboratory animal is rotated in a direction opposite to the twist direction to compensate for the twist of the optical fiber. A method for compensating for twisting of an optical fiber for a laboratory animal. 3. A cage floor for breeding an experimental animal by compensating for a twist of a lead wire connected to the experimental animal by a slip ring, detecting a twist of a tube connected to the experimental animal, detecting an amount and a direction of the twist, and breeding the experimental animal. A method for compensating a tube and a tube for torsion, comprising: rotating the tubes in directions opposite to the respective torsional directions to compensate for tube torsion. 4. Detecting torsion of a lead wire or a tube connected to an experimental animal, detecting the amount and direction of the torsion, and rotating the cage floor for breeding the experimental animal in a direction opposite to the torsion direction. A method for compensating for the twist of a laboratory animal lead wire or tube, wherein the twist of the wire or tube is compensated. 5. A base is provided with a rotatable floor surface, an open bottom of a cage for breeding experimental animals is arranged on the floor, and the cage is provided on the base, and a central portion of the upper surface of the cage is provided. A detector is provided to detect the torsion and direction of the tube or optical fiber by inserting a tube or optical fiber connected to the experimental animal into the hole, and a motor that rotates by inputting a signal from the detector is provided. A twist compensation device for a tube or an optical fiber for an experimental animal, wherein a rotation drive section is provided between the motor and the cage floor surface, and the cage floor surface is rotated in a direction opposite to a twist direction of the tube or the optical fiber. 6. A base is provided with a rotatable floor surface, an open bottom of a cage for breeding experimental animals is arranged on the floor, and the cage is provided on the base, and a central portion of the upper surface of the cage is provided. A hole is provided in the hole, a lead wire connected to the experimental animal in the hole, a slip ring that penetrates the tube and compensates for the twist of the lead wire, and a detection device that senses the twist and direction of the tube is provided. A laboratory motor, which is provided with a motor that rotates upon input of a signal from the motor, a rotation drive unit is provided between the motor and the cage floor, and the cage floor is rotated in the direction opposite to the tube twisting direction. Lead,
Tube twist compensator. 7. A base is provided with a rotatable floor surface, an open bottom of a cage for breeding experimental animals is disposed on the floor, and the cage is provided on the base, and a central portion of the upper surface of the cage is provided. A hole is provided in the hole, a lead wire connected to the experimental animal in the hole, a rotating shaft with a through hole penetrating the tube is equipped, and a detection device that senses the amount and direction of the twist of the lead wire and the tube is provided. A motor is provided to rotate by inputting a signal from the detection device, a rotation drive unit is provided between the motor and the cage floor, and the cage floor is rotated in a direction opposite to the twist direction of the lead wire and the tube. A device for compensating for the twist of a lead wire and a tube for a laboratory animal. 8. A rotary encoder equipped with a two-phase photosensor as a detecting device for detecting the amount and direction of the tube or optical fiber or lead wire and the tube twist, and a motor for rotating the cage floor is a stepping motor or a servo motor. The torsion compensator according to any one of claims 5 to 7, wherein the torsion compensation device is a lead wire for a laboratory animal, a tube, an optical fiber, or the like. 9. A rotation roller from the motor to the cage floor is fixed to a drive shaft of the motor, and a rubber roller whose outer periphery is in contact with a frame on an outer periphery of the cage floor is formed in a direction opposite to the twisting direction. 6. The method according to claim 5, wherein
The torsion compensator for laboratory animal lead wire, tube, optical fiber or the like according to any one of claims 8 to 8.