JP2780334B2 - Electrophoresis device - Google Patents
Electrophoresis deviceInfo
- Publication number
- JP2780334B2 JP2780334B2 JP1130586A JP13058689A JP2780334B2 JP 2780334 B2 JP2780334 B2 JP 2780334B2 JP 1130586 A JP1130586 A JP 1130586A JP 13058689 A JP13058689 A JP 13058689A JP 2780334 B2 JP2780334 B2 JP 2780334B2
- Authority
- JP
- Japan
- Prior art keywords
- electrode
- electrophoresis
- support
- tanks
- present
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Peptides Or Proteins (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Description
【発明の詳細な説明】 （イ） 産業上の利用分野 本発明は、生化学及び臨床分野においてタンパク、ア
ミノ酸などの分析に利用される電気泳動装置に関する。The present invention relates to an electrophoresis apparatus used for analyzing proteins, amino acids, and the like in the biochemistry and clinical fields.
（ロ） 従来技術 生化学物質の多くは分子内に多数の解離基を有する両
性電解質であり、pHの変化に応じてそれぞれに固有な電
荷の変化を示す。(B) Prior art Many biochemical substances are amphoteric electrolytes having a large number of dissociating groups in the molecule, and each of them exhibits a unique change in charge according to a change in pH.
この性質に基づいた電場のもとでの挙動の差によって
各成分の分離を行うのが電気泳動法でこれに使用する装
置として第２図のものがある。FIG. 2 shows an apparatus used for electrophoresis in which each component is separated by a difference in behavior under an electric field based on this property.
図中11は、ゲル支持体で、セルロースアセテート膜，
ろ紙，寒天，アガロース，デンプン，ポリアクリルアミ
ドゲルなどが支持される。In the figure, reference numeral 11 denotes a gel support, a cellulose acetate membrane,
Filter paper, agar, agarose, starch, polyacrylamide gel, etc. are supported.
12,13は、電極液入り電極槽で、槽内には各々電極
（図示せず）が挿入され、また電極槽とゲル支持体とは
ろ紙14,14′により電気連絡される。Reference numerals 12 and 13 denote electrode tanks containing an electrode solution. Electrodes (not shown) are inserted into the tanks, and the electrode tanks and the gel support are electrically connected to each other by filter papers 14 and 14 '.
かかる構成により、ゲル支持体上に塗布された試料イ
オンは通電によって対応する電極槽側に泳動され、移動
度，分子ふるい，吸着，電子浸透などの諸要因により分
離される。With this configuration, the sample ions applied on the gel support are electrophoresed on the corresponding electrode tank side by energization and separated by various factors such as mobility, molecular sieve, adsorption, and electron penetration.
（ハ） 発明が解決しようとする課題 しかしながら、上記装置では泳動中にろ紙が乾燥して
しまい、電流が流れなくなってしまったり、電解液がろ
紙より過剰にしみ出して泳動分離されるバンドが波打っ
たり歪んだりした。(C) Problems to be Solved by the Invention However, in the above-described apparatus, the filter paper dries during the electrophoresis, and the current stops flowing. Hammered and distorted.
また、ろ紙による電極槽とゲル支持体との架橋には位
置的な再現性がなかった。In addition, there was no positional reproducibility in the cross-linking between the electrode tank and the gel support by the filter paper.
そこで本発明は、上記課題を解決し、電極液とゲル支
持体との電気的接続を簡便に、かつ再現性良くする装置
を提供することを目的とする。Therefore, an object of the present invention is to solve the above-mentioned problems and to provide an apparatus for easily and reproducibly making an electrical connection between an electrode solution and a gel support.
（ニ）課題を解決するための手段 本発明は上記課題を解決するため、 試料を泳動分離させる泳動用支持体と、該泳動用支持
体上に設置される一対の電極槽と、該電極槽を泳動用支
持体上に位置決めする部材と、電極槽内に貫通して設け
られ、電極槽内の電極液と泳動用支持体とを電気的に接
続する多孔性の材料とを備えてなる。(D) Means for Solving the Problems In order to solve the above problems, the present invention provides a support for electrophoresis for separating a sample by electrophoresis, a pair of electrode vessels installed on the support for electrophoresis, and the electrode vessel. And a porous material that penetrates through the electrode tank and electrically connects the electrode solution in the electrode tank and the support for electrophoresis.
ここで、多孔性のフィルタとしては、例えば空孔径が
0.5〜２μm,空孔率30〜40％のセラミックフィルタが適
している。空孔径，空孔率が前記範囲をはずれると、電
解液の浸食が過小，過大となり、泳動に支障を来たすか
らである。Here, as a porous filter, for example, the pore diameter is
A ceramic filter with 0.5 to 2 μm and porosity of 30 to 40% is suitable. If the pore diameter and the porosity are out of the above ranges, the erosion of the electrolytic solution becomes too small or too large, which hinders the electrophoresis.
また、多孔性のフィルタの形状は、ゲル支持体への接
触を均一にするため断面がアール状になっているのが好
ましい。The shape of the porous filter is preferably round in order to make the contact with the gel support uniform.
（ホ） 作用 本発明は、ゲル支持体と電極液との接液の際余分なに
じみ出しがなく、しかも均一に接触させることができ
る。(E) Function In the present invention, when the gel support and the electrode solution come into contact with each other, there is no extra bleeding and uniform contact can be achieved.
（ヘ） 実施例 本発明の実施例を図面に基づいて説明する。(F) Example An example of the present invention will be described with reference to the drawings.
第１図（ａ）は、本発明に係る装置の全体斜視図，第
１図（ｂ）は横断面図である。FIG. 1 (a) is an overall perspective view of an apparatus according to the present invention, and FIG. 1 (b) is a cross-sectional view.
図中１は、温度調節可能なプレート（基板）であり、
支柱２が４本４隅に立っている。3a,3bは各々プラス，
マイナス用電極槽で槽内には電極液７（例えばトリス−
酢酸緩衝液）が充たされ、電極4a,4bが挿入される（な
お、第１図（ａ）では便宜上電極を図示していない）。In the figure, reference numeral 1 denotes a temperature-adjustable plate (substrate),
Four pillars 2 stand in four corners. 3a and 3b are each plus,
In the negative electrode tank, an electrode solution 7 (for example, Tris-
(Acetate buffer) and the electrodes 4a and 4b are inserted (the electrodes are not shown in FIG. 1 (a) for convenience).
また、電極槽3a,3b内底部には、気密性を保持しつつ
槽底部を貫通する形で多孔性フィルタ５（例えばセラミ
ックフィルタ）が固定されている。多孔性フィルタ５
は、電極液７がにじみ出ない程度の空孔径，空孔率のも
のが使用される。A porous filter 5 (for example, a ceramic filter) is fixed to the inner bottoms of the electrode tanks 3a and 3b so as to penetrate the bottoms of the tanks while maintaining airtightness. Porous filter 5
For the electrode, one having a pore diameter and a porosity that does not allow the electrode solution 7 to exude is used.
なお６は泳動用スラブゲルを示す。 Reference numeral 6 denotes a slab gel for electrophoresis.
以上の構成において本発明の動作を説明する。 The operation of the present invention in the above configuration will be described.
まず電極槽3a,3bを取り除いた状態でプレート（基
板）１上にスラブゲルをおく。プレート１は、外部温調
装置にて温調された循環水をプレート中に循環させた
り、ペルチエ素子などを利用して泳動中の温度コントロ
ールを行う。First, a slab gel is placed on a plate (substrate) 1 with the electrode tanks 3a and 3b removed. The plate 1 circulates circulating water whose temperature is controlled by an external temperature control device through the plate, or controls the temperature during electrophoresis using a Peltier element or the like.
次に、電極槽3a,3bを支柱２に差し込んでセットす
る。この支柱２により常にプレート１に対して多孔性フ
ィルタ５の位置は再現される。そして、電極液を槽内に
充たして電極間に電圧を印加して泳動を行う。電極槽3
a,3bの自重によるゲル６と多孔性フィルタ５は適度な押
さえつけの状態となり、均一な接触も確保される。Next, the electrode tanks 3a and 3b are inserted into the support 2 and set. The position of the porous filter 5 with respect to the plate 1 is always reproduced by the columns 2. Then, the electrophoresis is performed by filling the tank with the electrode solution and applying a voltage between the electrodes. Electrode tank 3
The gel 6 and the porous filter 5 due to their own weights a and 3b are in a state of being appropriately pressed, and uniform contact is ensured.
なお、以上の説明では、電極槽3a,3bは、支柱に人力
で差し込む構成であったが、モータなどを使って自動的
に槽が上下するようにしても良い。In the above description, the electrode tanks 3a and 3b are configured to be manually inserted into the columns, but the tanks may be automatically moved up and down using a motor or the like.
また、本発明は、垂直式泳動装置への適用も可能であ
る。Further, the present invention can be applied to a vertical electrophoresis apparatus.
（ト） 効果 本発明によれば、泳動中にろ紙が乾燥するなどの弊害
がなくなるので、分解能が向上する。(G) Effect According to the present invention, the adverse effects such as drying of the filter paper during electrophoresis are eliminated, so that the resolution is improved.
また、ろ紙の架橋などの操作がなく、取り扱いが非常
に容易になると共に泳動分離バンドの再現性も向上す
る。Further, there is no operation such as cross-linking of the filter paper, so that the handling becomes very easy and the reproducibility of the electrophoresis separation band is improved.
更に、電気泳動装置の自動化にも対応できる。 Furthermore, it can respond to automation of the electrophoresis apparatus.
第１図（ａ）は、本発明に係る装置の全体概略図第１図
（ｂ）は、本発明に係る装置の横断面図を示す。第２図
は，従来図である。 １……プレート（基板）、２……支柱 3a,3b……電極槽、５……多孔性フィルタ ６……泳動用スラブゲルFIG. 1 (a) is an overall schematic view of the device according to the present invention. FIG. 1 (b) is a cross-sectional view of the device according to the present invention. FIG. 2 is a conventional diagram. DESCRIPTION OF SYMBOLS 1 ... Plate (substrate), 2 ... Support 3a, 3b ... Electrode tank, 5 ... Porous filter 6 ... Slab gel for electrophoresis
Claims (1)
泳動用支持体上に設置される一対の電極槽と、該電極槽
を泳動用支持体上に位置決めする部材と、電極槽内に貫
通して設けられ電極槽内の電極液と泳動用支持体とを電
気的に接続する多孔性の材料とを備えてなる電気泳動装
置。An electrophoresis support for electrophoretically separating a sample, a pair of electrode tanks provided on the electrophoresis support, a member for positioning the electrode tank on the electrophoresis support, An electrophoresis apparatus, comprising: a porous material that is provided so as to penetrate the substrate and electrically connects the electrode solution in the electrode tank and the electrophoresis support.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1130586A JP2780334B2 (en) | 1989-05-24 | 1989-05-24 | Electrophoresis device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1130586A JP2780334B2 (en) | 1989-05-24 | 1989-05-24 | Electrophoresis device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH02309248A JPH02309248A (en) | 1990-12-25 |
| JP2780334B2 true JP2780334B2 (en) | 1998-07-30 |
Family
ID=15037753
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1130586A Expired - Fee Related JP2780334B2 (en) | 1989-05-24 | 1989-05-24 | Electrophoresis device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2780334B2 (en) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE452853B (en) * | 1986-02-13 | 1987-12-21 | Pharmacia Ab | WAY TO SUPPLY BUFFER SOLUTIONS FOR ELECTROPHORETIC SEPARATION |
-
1989
- 1989-05-24 JP JP1130586A patent/JP2780334B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH02309248A (en) | 1990-12-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |