JP2000028578A - Plate-like electrode for electric transfer, and electric transfer method therefor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To realize an electric transfer being convenient and also advantageous in a cost by utilizing an electrolytic device and using a simple tool comprising a plurality of plate-like electrodes, particularly a plate-like electrode having an L-shaped form. SOLUTION: At transfer, two sheets of L-shaped plate-like electrode 7 is installed on an electrolyte container 1 at an arrangement overlapped in parallel. Next, a gel 8 separated by electrolyting a substance and a film which is an object to be transferred are closely adhered and it is clamped between both plate-like electrodes 7 of a platform 2. Moreover, a buffer solution 10 is accumulated in the electrolytic container, however, the buffer solution 10 does not arrive at the platform 2 which is different from the time of an electrolysis. Namely, it is important that both plate-like electrode 7, a gel 8 and a film 9 are not impregnated. Then, the electrolytic device is activated and an electric transfer is carried out by energizing the electrolytic container 1.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for electrically transferring an interelectrode substance and a plate-like electrode used in the method. More specifically, in order to expand its operability and simplicity, an electrode is characterized by using a plate-shaped electrode, particularly a simple instrument comprising two L-shaped plate-shaped electrodes, using an electrophoresis apparatus. The present invention relates to a method for electrically transferring an intermediary substance and a plate-like electrode for electrical transfer, and particularly to an apparatus for electrical transfer comprising two L-shaped plate-like electrodes.

[0002]

2. Description of the Related Art Electrophoresis is a universal technique used in a wide range of industrial fields, such as basic research in biochemistry and various tests. In particular, electrophoresis devices used for nucleic acid analysis have laid the foundations of today's molecular biology in conjunction with the development of agarose gels. On the other hand, as an analysis means after electrophoresis, a substance on a gel, particularly a nucleic acid, is transferred onto a membrane such as a nylon membrane, and operations such as hybridization and antigen-antibody reaction are performed on the membrane.

As a method for transferring a substance on a gel, a method in which a film and a gel are brought into close contact with each other and transferred using a capillary phenomenon, and an electric transfer in which a substance is electrically transferred to a film using electric charge of the substance. There is a law. Among them, the electric transfer method has an advantage that the transfer speed is faster than the method utilizing the capillary phenomenon, but requires an expensive dedicated energizing device.

[0004]

SUMMARY OF THE INVENTION An object of the present invention is to develop an electric transfer method which is simple in terms of the apparatus necessary for the electric transfer of a substance on a gel as described above and which is advantageous in terms of cost. is there. That is, it is expensive as before,
By making it possible to use the electrophoresis apparatus used for analysis earlier as it is, instead of using an electric transfer device dedicated to electrical transfer that requires some technology in terms of operation, An object of the present invention is to provide a greatly simplified electric transfer method and a plate-like electrode used therefor.

[0005]

Means for Solving the Problems The present inventors have made intensive studies in view of the above circumstances, and as a result, have made use of the electrophoresis apparatus used for analysis to form two plate-shaped electrodes, particularly an L-shaped electrode. The present inventors have found that the use of a simple instrument including two plate-shaped electrodes can perform simple and convenient cost-effective electrical transfer, and have reached the present invention.

That is, the present invention relates to a plate-like electrode used for electrically transferring an interelectrode substance, wherein the plate-like electrode is provided on a platform for holding a gel for electrophoresis. 2 electrodes in parallel
A plate-like electrode for electrical transfer characterized in that it is installed and used to sandwich the gel in an overlapping arrangement.
The shape of the plate-like electrode is not particularly limited as long as the gel can be sandwiched between and energized.
The shape may be a letter shape, a simple flat shape, or the like. Particularly preferably, it is L-shaped because a convex platform of a horizontal submarine electrophoresis apparatus can be used.

[0007] The present invention also provides a horizontal submarine electrophoresis apparatus having an arrangement arranged in parallel on a platform.
This is a method for electrically transferring an interelectrode substance, comprising installing two L-shaped plate-like electrodes sandwiching an electrophoresed gel, and energizing the L-shaped plate-like electrodes. It is particularly useful when the interelectrode substance is a nucleic acid.

In the present invention, the interelectrode substance refers to a biomolecule having a charge. Specific examples thereof include nucleic acids and proteins that are biopolymers. The electric transfer method of the present invention is particularly useful when the interelectrode substance is a nucleic acid. Further, in the present invention, the electric transfer refers to an electric transfer of a substance between electrodes to a target, and specifically refers to a transfer step in an operation such as membrane blotting or hybridization. .

The electrophoresis apparatus that can be used in the present invention is not particularly limited as long as it has a so-called platform for holding a carrier for electrophoresis such as agarose gel or polyacrylamide gel.
In particular, it is preferable to use a horizontal submarine electrophoresis apparatus. Specifically, the horizontal type submarine electrophoresis apparatus is provided with an electrophoresis tank which is an electrophoresis tank portion, a convex platform formed almost at the bottom of the electrophoresis tank, and near both ends of the bottom of the electrophoresis tank. Buffer during electrophoresis, a gel is placed on the convex platform during electrophoresis, and immersed in the buffer. Has been
At the time of electrical transfer, it is preferable that two L-shaped plate-shaped electrodes sandwiching the gel are provided in an arrangement overlapping in parallel on the platform, and the L-shaped plate-shaped electrodes are energized.

[0010] The material of the plate-like electrode in the present invention is not particularly limited as long as it has conductivity, and specific examples thereof include a platinum-deposited plate, a titanium alloy plate, and a conductive ceramic. Among them, a platinum vapor-deposited plate is particularly preferable because a platinum wire is usually used for electrophoresis. In addition, even when a non-conductive material is used, it can be used if surface treatment such as plating is performed, or if conductivity is imparted by treatment such as placing a conductive wire on the surface or inside. is there. However, the problem of corrosion must be considered in terms of the life of the equipment.

[0011] According to the electric transfer method of the present invention, an expensive dedicated energizing device conventionally used is not required.
Using the electrophoresis device used for analysis as it is and simply preparing a new simple instrument consisting of two plate electrodes,
Electrical transfer of inter-electrode substances such as nucleic acids becomes possible.

[0012]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Preferred embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a schematic view of an embodiment of an apparatus used in the electric transfer method of the present invention. Note that the present invention is not particularly limited to FIG.

In FIG. 1A, an electrophoresis tank 1 is a part of an electrophoresis tank of a horizontal type submarine electrophoresis apparatus, and a convex portion formed substantially at the center of a bottom serves as a platform 2.
At the time of electrophoresis, the gel 3 is placed on the platform 2. During electrophoresis, a buffer 4 for electrophoresis is stored in the electrophoresis tank 1 and the gel is immersed. A negative electrode 5 and a positive electrode 6 made of platinum wire are provided near both ends in the electrophoresis tank 1 at the bottom in the electrophoresis direction.

Further, at the time of transfer, two L-shaped plate-like electrodes 7 are placed in the electrophoresis tank 1 so as to overlap in parallel on the platform 2 as shown in FIG. Next, the gel 8 from which the substance is electrophoresed and separated and the film 9 to be transferred are brought into close contact with each other and sandwiched between the two plate-shaped electrodes 7 of the platform 2. Further, the buffer 10 is stored in the electrophoresis tank 1, but unlike the electrophoresis, the buffer 10 does not reach the platform 2, that is, the two-plate electrode 7, the gel 8, and the membrane 9 are not immersed. is important. Then, the electrophoresis apparatus is activated and the electrophoresis tank 1 is energized to perform the electric transfer.

[0015]

As described above, according to the plate electrode for electric transfer and the electric transfer method of the present invention, it is possible to realize electric transfer that is simple and advantageous in cost. That is, the present invention provides two plate-shaped electrodes,
By using a plate-shaped electrode having a letter-shaped shape, an electrophoresis device used in a stage before electrical transfer can be used as it is as a current-carrying device.

[Brief description of the drawings]

FIG. 1 is a schematic view of an embodiment of an apparatus used for an electric transfer method of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Electrophoresis tank 2 Platform 3 Gel 4 Buffer solution 5 Negative electrode 6 Positive electrode 7 L-shaped plate electrode 8 Gel after electrophoresis 9 Membrane 10 Buffer solution

Claims (4)

[Claims] 1. An electrophoretic device having a platform for holding a gel for electrophoresis, wherein the plate-like electrodes are used in parallel to form a plate-like electrode used for electrically transferring an interelectrode substance. A plate-like electrode for electrical transfer, wherein the plate-like electrode is used to sandwich and hold the gel in an overlapping arrangement of two sheets. 2. The plate electrode for electrical transfer according to claim 1, wherein the plate electrode has an L-shape. 3. An L-shaped plate-like electrode for sandwiching an electrophoresed gel is provided in an arrangement overlapping in parallel on a platform in a horizontal type submarine electrophoresis apparatus, and a current is supplied to the L-shaped plate-like electrode. A method for electrically transferring an inter-electrode material, comprising the steps of: 4. The method according to claim 3, wherein the interelectrode substance is a nucleic acid.