JP2770119B2 - Pest control material - Google Patents
Pest control materialInfo
- Publication number
- JP2770119B2 JP2770119B2 JP5341443A JP34144393A JP2770119B2 JP 2770119 B2 JP2770119 B2 JP 2770119B2 JP 5341443 A JP5341443 A JP 5341443A JP 34144393 A JP34144393 A JP 34144393A JP 2770119 B2 JP2770119 B2 JP 2770119B2
- Authority
- JP
- Japan
- Prior art keywords
- soil
- bacterial wilt
- present
- control material
- pseudomonas fluorescens
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明はカニ殻とシュウドモナス
・フルオレッセンスとの混合物からなる青枯病防除材に
関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a material for controlling bacterial wilt comprising a mixture of crab shell and Pseudomonas fluorescens.
【０００２】[0002]
【従来の技術】連作によりナス科作物はしばしば青枯病
にかかり、農家に多大な損失を与えている。BACKGROUND OF THE INVENTION Successful cropping often causes solanaceous crops to wilt, causing great losses to farmers.
【０００３】青枯病の防除方法としてはいくつかの方法
が試みられている。例えば、その方法としてクロルピク
リンや臭化メチルなどの薬剤による土壌殺菌があげられ
る。しかし、これら薬剤による土壌殺菌は、有益な菌ま
で無差別に殺し、土壌微生物の構成を崩し他の病害を誘
発し、作物の健全な生育を阻害することがある。また、
これら薬剤は刺激臭があり、コスト高であるばかりでな
く、繰り返し施用による殺菌剤の土壌残汚染の問題があ
る。[0003] Several methods have been tried as a method for controlling bacterial wilt. For example, the method includes soil sterilization with a drug such as chlorpicrin or methyl bromide. However, soil disinfection with these agents indiscriminately kills even beneficial bacteria, disrupts the composition of soil microorganisms, induces other diseases, and can hinder the healthy growth of crops. Also,
These chemicals have an irritating odor, are not only costly, but also have the problem of soil contamination of the fungicide in the soil due to repeated application.
【０００４】他の防除方法としては、青枯病菌であるシ
ュウドモナス・ソラナシラム(Pseudomonas solanacearu
m)の拮抗菌を増殖させるために畜産糞、おがくず、稲藁
等の有機物を施用する方法や、青枯病に対する耐病性を
高めるために、病害に強い苗に接ぎ木をする方法も行わ
れている。しかし有機物の施用は一定した効果が期待で
きず、接ぎ木においても、効果はあるが手間がかかりコ
スト高になる。また、微生物を利用して連作障害を抑
制、防除する方法もいくつか報告されている。[0004] As another control method, Pseudomonas solanacearu, a bacterial wilt fungus, is used.
m) The method of applying organic matter such as livestock droppings, sawdust, rice straw to multiply the antagonistic antimicrobial activity, and the method of grafting to disease-resistant seedlings to increase disease resistance against bacterial wilt have also been carried out. I have. However, the application of organic substances cannot be expected to have a certain effect, and even when grafting is used, it is effective but takes time and costs. In addition, several methods have been reported for controlling and controlling continuous cropping disorders using microorganisms.
【０００５】例えば特開昭61-200193号公報には各種の
微生物の生菌体または胞子を使用した根圏土壌改良剤が
開示され、その中の一つにシュウドモナス・フルオレッ
センス(Pseudomonas fluorescens)が例示され、微生物
の生育促進資材としてカニ殻が例示されているが後述す
る本発明を開示するものではない。また、特開平5-8044
4号公報にはシュウドモナス属の新菌株を用いて、立枯
病、青枯病を防除する方法が開示されている。しかし、
一般に拮抗菌を利用した場合土壌中での生存率が低いこ
とや、失活等により充分な効果が得られていないため実
用化には至っていない。For example, Japanese Patent Application Laid-Open No. 61-200193 discloses a rhizosphere soil conditioner using viable cells or spores of various microorganisms, one of which is Pseudomonas fluorescens. As an example, a crab shell is exemplified as a material for promoting the growth of microorganisms, but does not disclose the present invention described later. Also, JP-A-5-8044
No. 4 discloses a method for controlling damping-off and bacterial wilt using a new strain of the genus Pseudomonas. But,
In general, when an antibacterial agent is used, it has not been put to practical use because its survival rate in soil is low and sufficient effects have not been obtained due to inactivation.
【０００６】[0006]
【発明が解決しようとする課題】本発明はかかる現状に
鑑みなされたものであって、本発明の目的は青枯病に対
して抑制効果が高く、低コストで繰り返して施用しても
環境に影響のない防除材を提供することにある。DISCLOSURE OF THE INVENTION The present invention has been made in view of such circumstances, and it is an object of the present invention to provide a highly effective control against bacterial wilt and to reduce environmental costs even when repeatedly applied at low cost. An object of the present invention is to provide a control material having no influence.
【０００７】[0007]
【課題を解決するための手段】即ち本発明はカニ殻とシ
ュウドモナス・フルオレッセンスとの混合物からなる青
枯病防除材に関する。That is, the present invention relates to a material for controlling bacterial wilt comprising a mixture of crab shell and Pseudomonas fluorescens.
【０００８】本発明者らはこのシュウドモナス・フルオ
レッセンスの青枯病菌に対する拮抗性をin vitroで調べ
た結果、拮抗性が認められたが、このシュウドモナス・
フルオレッセンスを青枯病で汚染されていた土壌に施用
しても作物の青枯病を防除することはできなかった。The present inventors have examined in vitro the antagonism of Pseudomonas fluorescens against bacterial wilt, and found that the antagonism was confirmed.
Application of fluorescence to soil contaminated with blight did not control the blight of the crop.
【０００９】そこでこのin vitroで認められているシュ
ウドモナス・フルオレッセンスの青枯病に対する拮抗性
を青枯病汚染土壌で発現させる方法について検討し本発
明を完成したものである。Accordingly, the present invention was completed by studying a method for expressing the antagonisticity of Pseudomonas fluorescens against bacterial wilt observed in vitro in bacterial wilt contaminated soil.
【００１０】[0010]
【作用】以下に本発明を詳記する。本発明の青枯病防除
剤の製造方法としては、あらかじめカニ殻を1〜5mm程度
に砕き、このカニ殻にシュウドモナス・フルオレッセン
スの培養物を混合するのが最も安価である。The present invention will be described below in detail. As a method for producing the agent for controlling bacterial wilt of the present invention, it is most inexpensive to crush crab shells in advance to about 1 to 5 mm and mix a culture of Pseudomonas fluorescens with the crab shells.
【００１１】シュウドモナス・フルオレッセンスの培養
物は、肉エキス、ペプトン、酵母エキス、グルコース、
しょ糖、リン酸カリウム、硫酸マグネシウム等の栄養源
を含んだ培地で、pHを7付近に調製し、温度約30℃で培
養、増殖することにより製造する。The culture of Pseudomonas fluorescens comprises meat extract, peptone, yeast extract, glucose,
It is manufactured by culturing and growing at a temperature of about 30 ° C. in a medium containing nutrients such as sucrose, potassium phosphate, and magnesium sulfate at a pH of about 7.
【００１２】本発明防除材はシュウドモナス・フルオレ
ッセンスとカニ殻との混合物の菌濃度が104CFU/g以上で
あることが効果発現の点から好ましい。[0012] The control material of the present invention is preferably a mixture of Pseudomonas fluorescens and crab shells having a bacterial concentration of 10 4 CFU / g or more from the viewpoint of achieving the effect.
【００１３】本発明防除材の製造に際し、炭素源、窒素
源、リン酸カリ等の栄養源を加えることが出来ることは
勿論、ピートモス、バーミキュライト等の微生物着生材
兼土壌改良材を加えることは取り扱いあるいは効果の点
で更に好ましい。In producing the control material of the present invention, it is possible to add a nutrient source such as a carbon source, a nitrogen source and potassium phosphate, and also to add a microorganism-implanting material and a soil improving material such as peat moss and vermiculite. It is more preferable in terms of handling and effects.
【００１４】このようにして調製した本発明防除材の使
用方法としては、作物植え付け前に土壌に施用混合する
ことが望ましい。施用量は防除材の菌濃度により異なる
が一般的には200Kg/10a以上が好ましい。また、別法と
して青枯病初期の作物に施用することもできるが効果は
作物植え付け前の施用に比べて劣る。As a method of using the thus-prepared controlling agent of the present invention, it is desirable to apply and mix the soil before soil planting. The application rate varies depending on the bacterial concentration of the control material, but is generally preferably 200 kg / 10a or more. Alternatively, it can be applied to crops in the early stage of bacterial wilt, but the effect is inferior to that before crop planting.
【００１５】本発明防除材は拮抗性細菌及びカニ殻を主
材として使用する物であるため、堆肥あるいは肥料と全
く同様に取り扱うことができ、繰り返し施用しても農薬
等のような土壌汚染もなく人畜に対する有害作用もな
い。Since the control material of the present invention mainly uses antagonistic bacteria and crab shells, it can be handled in exactly the same manner as compost or fertilizer. Even if it is applied repeatedly, soil contamination such as pesticides can be prevented. No adverse effects on humans and animals.
【００１６】[0016]
【実施例】以下に本発明を実施例により更に説明する
が、本発明はこれらに限定されるものではない。また、
％は特に断らない限り全て重量％を示す。EXAMPLES The present invention will be further described below with reference to examples, but the present invention is not limited to these examples. Also,
All percentages are by weight unless otherwise specified.
【００１７】（使用材料） 青枯病菌---青枯病で枯死したトマト茎内から分離し
たシュウドモナス・ソラナシラム 青枯病菌の培養物---の青枯病菌をＰＰＧＢ培地
（ジャガイモ200ｇからのポテト煎汁液にペプトン５
ｇ、グルコ−ス５ｇ、燐酸２ナトリウム12水塩３ｇ、燐
酸一カリウム0.5ｇ、食塩３ｇを溶かした液を蒸留水で1
000mlにしたもの)で培養したもの。 青枯病含有土壌---風乾し滅菌した加古川沖積土壌に
滅菌水とを加え、水分20％になるよう混合したもの。 カニ殻---1〜5mmに粉砕したもの。 本発明防除材１---シュウドモナス・フルオレッセン
スIFO-13922をＰＰＧＢ培地で培養し、この培養物を各
種割合でカニ殻と混合したもの。 本発明防除材２---シュウドモナス・フルオレッセン
スIFO-14808をＰＰＧＢ培地で培養し、この培養物を各
種割合でカニ殻と混合したもの。(Materials used) Bacterial wilt fungus --- a culture of Pseudomonas solanaciram B. wilt isolated from the tomato stems that have died due to the wilt disease was transferred to a PPGB medium (potato from 200 g of potato). Peptone 5 in the decoction
g, glucose 5 g, disodium phosphate 12 hydrate 3 g, monopotassium phosphate 0.5 g, and sodium chloride 3 g were dissolved in distilled water.
000 ml). Bacterial blight-containing soil --- Sterile water added to air-dried and sterilized alluvial soil of Kakogawa and mixed to a water content of 20%. Crab shell --- crushed to 1-5mm. Inventive control material 1 --- Pseudomonas fluorescens IFO-13922 is cultured in a PPGB medium, and this culture is mixed with crab shells in various ratios. Inventive control material 2 --- Pseudomonas fluorescens IFO-14808 cultured in PPGB medium, and this culture is mixed with crab shells in various ratios.
【００１８】 [0018]
【００１９】（実施例１）の青枯病含有土壌125gを15
8mmの大型シャーレに採り、これに表１に示す資材を表
２の使用割合で加えて混合した。この土壌を30℃で14日
間培養し、青枯病菌数を調べた。その結果を表２に示
す。尚、青枯病菌の検出には原・小野培地を用い30℃で
培養２日目のコロニー数を計測した。[0025] 125 g of the soil containing bacterial wilt of Example 1
The mixture was taken into a large petri dish of 8 mm, and the materials shown in Table 1 were added thereto at the usage ratios shown in Table 2 and mixed. This soil was cultured at 30 ° C. for 14 days, and the number of bacterial wilts was examined. Table 2 shows the results. For the detection of bacterial wilt, the number of colonies on the second day of culture at 30 ° C. was counted using the original Ono medium.
【００２０】[0020]
【表１】 上表から明らかなように本発明防除材を用いた場合、著
しく青枯病菌が減少することが判る。[Table 1] As is clear from the above table, it can be seen that the use of the control material of the present invention significantly reduces bacterial wilt.
【００２１】（実施例２）実施例１に於ける試験後の試
験区の土壌100gをポリビーカーに移し、本葉2〜3枚のト
マト幼苗を各３本ずつ移植し、30℃温度75％の人工気象
器中で栽培した。14日後の状態を表３に示す。(Example 2) 100 g of the soil in the test plot after the test in Example 1 was transferred to a polybeaker, and 2-3 tomato seedlings each having 2-3 true leaves were transplanted at 30 ° C and 75% temperature. Cultivated in an artificial weather vessel. Table 3 shows the condition after 14 days.
【００２２】 [0022]
【００２３】（実施例３）滅菌した加古川沖積土壌40Kg
を63.5cm×37.5cm×22.5cmのプランターに入れトマト10
本を植えた。このプランターをハウス内に置き、の培
養物500ml(8.4×108CFU/ml)をトマトの根元に罐注し
た。ハウス内の夜温は20℃以上、日中は20〜35℃に保ち
感染発病させ、この土壌でトマトの植え付けを繰り返
し、土壌に生息している青枯病菌による連作障害がでる
状態にした。こうして作成した病土の青枯病菌数は実施
例１と同様に測定して5.5×105CFU/g乾土であった。こ
の病土を良く混ぜ６等分し、2.5Kgを分取し1/5000aワグ
ネルポットにいれ、化成肥料(N-P-Kの成分が各10％含
有)10gを施用混合した。表４に示す資材を添加混合し上
記ハウス内に14日静置した後、本葉2〜3枚のトマト幼苗
を各４本ずつ移植し、夜温は20℃以上、日中は20〜35℃
に保ち栽培した。移植14日後の状態を表５に示した。(Example 3) 40 kg of sterilized alluvial soil of Kakogawa
Into a 63.5cm x 37.5cm x 22.5cm planter
I planted a book. The planter was placed in a house, and 500 ml of the culture (8.4 × 10 8 CFU / ml) was poured into the root of the tomato. Infection was caused by keeping the night temperature in the house at 20 ° C or higher and 20-35 ° C during the day, and tomato planting was repeated in this soil, so that continuous cropping failure caused by the bacterial wilt fungus in the soil occurred. The bacterial wilt count of the diseased soil thus prepared was 5.5 × 10 5 CFU / g dry soil as measured in the same manner as in Example 1. The diseased soil was mixed well and divided into 6 equal portions, 2.5 kg was collected and placed in a 1 / 5000a Wagner pot, and 10 g of a chemical fertilizer (containing 10% of each component of NPK) was applied and mixed. After the materials shown in Table 4 were added and mixed and allowed to stand in the above house for 14 days, four tomato seedlings each having 2-3 leaves were transplanted, the night temperature was 20 ° C or higher, and the daytime was 20 to 35 days. ° C
And cultivated. The state 14 days after the transplantation is shown in Table 5.
【００２４】 [0024]
【００２５】 [0025]
【００２６】[0026]
【発明の効果】本発明は青枯病に対して抑制効果が高く
低コストで繰り返して施用しても環境に影響のない優れ
た防除材である。Industrial Applicability The present invention is an excellent control material which has a high control effect on bacterial wilt and does not affect the environment even when repeatedly applied at low cost.
Claims (1)
ンスとの混合物からなる青枯病防除材。1. A bacterial wilt control material comprising a mixture of crab shell and Pseudomonas fluorescens.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5341443A JP2770119B2 (en) | 1993-12-09 | 1993-12-09 | Pest control material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5341443A JP2770119B2 (en) | 1993-12-09 | 1993-12-09 | Pest control material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH07165523A JPH07165523A (en) | 1995-06-27 |
| JP2770119B2 true JP2770119B2 (en) | 1998-06-25 |
Family
ID=18346127
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5341443A Expired - Fee Related JP2770119B2 (en) | 1993-12-09 | 1993-12-09 | Pest control material |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2770119B2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108713461A (en) * | 2018-04-24 | 2018-10-30 | 奚正华 | A kind of solanaceous vegetable bacterial wilt control method |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2614913B2 (en) * | 1989-02-14 | 1997-05-28 | 農林水産省 | Pseudomonas bacterium P-4 strain, soil disease controlling agent and soil disease controlling method |
| JP2915960B2 (en) * | 1990-04-19 | 1999-07-05 | コープケミカル株式会社 | How to control Fusarium disease |
-
1993
- 1993-12-09 JP JP5341443A patent/JP2770119B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH07165523A (en) | 1995-06-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Jambhulkar et al. | Delivery systems for introduction of microbial inoculants in the field | |
| Gül et al. | Rhizobacteria promoted yield of cucumber plants grown in perlite under Fusarium wilt stress | |
| Yogev et al. | Suppression of bacterial canker of tomato by composts | |
| CN107325821A (en) | A kind of biomass carbon base microbe microbial inoculum for preventing and treating watermelon blight and preparation method and application | |
| US11674118B2 (en) | PGPR compositions and methods for improved cultivation of tomato and potato species | |
| Leite et al. | Acclimatization of Pouteria gardeneriana Radlk micropropagated plantlets: Role of in vitro rooting and plant growth–promoting bacteria | |
| Sheikh et al. | Efficacy of Bacillus thuringiensis and Rhizobium meliloti with nursery fertilizers in the control of root infecting fungi on mung bean and okra plants | |
| KR100566264B1 (en) | 3101 Biocontrol of plant diseases using novel endophytic isolate of Pseudomonas fluorescence MC3101 | |
| JP3192577B2 (en) | Pest control material | |
| JP3132195B2 (en) | New microorganism and plant disease control agent | |
| WO1991000012A1 (en) | Microbes for controlling pests | |
| WO2002087344A1 (en) | Biological control of soil dwelling pests | |
| JP2019165676A (en) | Plant disease control agent and method of controlling plant disease | |
| JP2009096721A (en) | Method of controlling plant insect pest derived from plant parasitic nematode and agent for controlling the plant insect pest | |
| KR20050034000A (en) | Biocontrol of plant diseases using novel endophytic isolate of burkholderia vietnamensis mc1404 | |
| JP2770119B2 (en) | Pest control material | |
| US5676726A (en) | Matrix and agricultural materials | |
| CN102599196A (en) | Tall fescue growth adjusting method of employing bacillus thuringiensis fermentation broth | |
| KR20050033999A (en) | Biocontrol of plant diseases using novel epiphytic isolate of pseudomonas putida cb11 | |
| CN102647910A (en) | Plant-rearing agent, plant disease resistance inducer, and plant disease control method | |
| CN111995472A (en) | Disease-resistant growth-promoting type composite slow-release fertilizer and preparation method thereof | |
| Pastor et al. | Inoculation of tomato roots with single and mixed suspensions of Trichoderma harzianum ITEM 3636 conidia and Pseudomonas putida PCI2 cells | |
| JPH09194316A (en) | Agent for controlling soil blight of plant of family solanaceae and controlling method | |
| JP3540155B2 (en) | Brassicaceae clubroot control material | |
| KR100616289B1 (en) | Serratia marcescens THR-2 isolated from plants and a destroying method of mite using the strain |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |