JP2745047B2 - Apparatus and method for separating magnetotactic bacteria - Google Patents
Apparatus and method for separating magnetotactic bacteriaInfo
- Publication number
- JP2745047B2 JP2745047B2 JP62081912A JP8191287A JP2745047B2 JP 2745047 B2 JP2745047 B2 JP 2745047B2 JP 62081912 A JP62081912 A JP 62081912A JP 8191287 A JP8191287 A JP 8191287A JP 2745047 B2 JP2745047 B2 JP 2745047B2
- Authority
- JP
- Japan
- Prior art keywords
- magnetotactic
- bacteria
- magnetotactic bacteria
- separating
- sediment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、水底沈殿物中に生息する走磁性細菌の効率
的な分離装置及び方法に関する。
〔従来の技術とその問題点〕
微好気性細菌の一種である走磁性細菌は淡水や海水中
に生息し、地磁気を感じ磁力線に沿って泳ぐ性質を有す
る細菌として知られている（Science,190,337〜379（19
75））。この走磁性細菌は、磁鉄鉱からなる磁気微粒子
を鎖状に配列された状態で菌体内に含んでいるが、この
磁気微粒子は寸法が500〜1500Å程度と微小で単磁区構
造を有するものである。このような磁気微粒子は、磁気
記録材料、医療分野における薬剤キャリア等として期待
されているが、反面人工的に合成することは不可能に近
い。そこで、走磁性細菌を効率良く培養し、そして分離
する装置及び方法が求められている。
走磁性細菌の効率的な培養方法としては、本発明者ら
が特願昭61-273619号で提案した方法がある。走磁性細
菌の培養は一般に水底沈殿物、即ち泥の中で行なわれる
ので、培養、増殖させた走磁性細菌は水底沈殿物から分
離、濃縮する必要がある。従来、水底沈殿物からの走磁
性細菌の分離方法又は装置としては、例えば、特開昭61
-56074号、同61-81778号及び同61-81779号の記載のもの
が知られている。
〔発明が解決しようとする問題点〕
しかし、上記の従来知られている走磁性細菌の分離装
置及び方法は、使用する装置が複雑であるわりには分離
効率が低いという欠点を有している。
そこで、本発明の目的は、簡単な装置で効率よく走磁
性細菌を分離できる装置及び方法を提供することにあ
る。
〔問題点を解決するための手段〕
本発明は、前記の目的を達成するものとして、水性沈
殿物及び走磁性細菌を含む水から走磁性細菌を分離する
ための装置であって、
上部に前記の水性沈殿物及び走磁性細菌を含む水を投
入する入口を有し、下部にその出口を有し、その内部に
ほぼ鉛直方向に延びる流路が形成されている、非磁性材
料からなる容器と、
該容器内に鉛直方向に設けられた、走磁性細菌は通過
することができるが水底沈殿物は通過することができな
い多孔質隔膜とからなり、
前記容器内は該隔膜により前記の流路と、該隔膜を通
過した走磁性細菌を含む水を収容する走磁性細菌回収室
とに仕切られており、さらに該走磁性細菌回収室の下部
には細菌回収口が設けられており、
さらに、前記容器の外側に、該容器をはさむようにか
つ前記多孔質隔膜に対してほぼ垂直な方向に磁場が加え
られるように配置された一対の磁石を備えてなる、走磁
性細菌分離装置を提供するものである。
さらに、本発明は、上記の装置を用いて、前記一対の
磁石により前記多孔質隔膜に対してほぼ垂直な方向に磁
場を加えながら前記の流路に水底沈殿物及び走磁性細菌
を含む水を流し、これにより前記走磁性細菌回収室に走
磁性細菌を分離する工程を含む走磁性細菌の分離方法を
提供する。
本発明の装置及び方法において、前記の流路を仕切る
多孔質隔膜としては、例えば、濾紙、ガラスフィルタ
ー、セラミックフィルター等の非金属材料からなる隔
膜；アルミニウム等の非磁性材料からなる隔膜を用いる
ことができ、孔径が水底沈殿物の通過は妨げるが走磁性
細菌を通過させる大きさであることが必要で、通常約１
〜10μｍの範囲のものでよい。
本発明で多孔質隔膜に垂直な方向に磁場を加えるに
は、例えば、ヘルムホルツコイル、ソレノイド等の電磁
石；あるいはその他のフェライト、金属等でできた永久
磁石を使用することができる。
〔実施例〕
以下、本発明の装置及び方法を実施例に即して具体的
に説明する。
第１図に斜視図を示す装置を用いて本発明の方法を実
施する例を説明する。
第１図の装置は、分離器１とこの分離器１をはさむ位
置に配置された一対のヘルムホルツコイル2,2′とから
構成されている。第２図は分離器１の平面図である。
分離器１は、大体直方体状の容器からなり、上部から
走磁性細菌（以下、「細菌」という）及び水底沈殿物を
含む水（以下、「泥水」という）を注入すると、内部で
細菌が分離され、その後の泥水が下部の排出口３から出
てくる。したがって、分離器１内では、上方から下方へ
垂直方向の流路が形成される。分離器１の内部は流路の
方向に沿って（即ち縦方向に）設けられたナイロンメッ
シュ４及び濾紙５により３室に分かれている。泥水はナ
イロンメッシュ４の左側の室６に注入される。泥水中の
水底沈殿物（泥）はナイロンメッシュ４及び濾紙５によ
り右側の室（走磁性細菌回収室）７へは移行できない
が、細菌はヘルムホルツコイル2,2′により形成された
磁場の方向（濾紙５に垂直な方向で北指向性菌の場合回
収室７側がＳ極となる方向）へ移動し、水とともに濾紙
５を通過して回収室７に入る。ナイロンメッシュ４は必
ずしも必要ないが、多孔質隔膜として濾紙を用いる場合
には濾紙のみでは泥の負荷に対し耐えられないことがあ
るのでナイロンメッシュなどを併用することが望まし
い。こうして、磁場を加えながら泥水を連続的に注入す
ると、細菌は次々に回収室７に分離され、濃縮されるこ
とになる。回収室７に分離される細菌は分離され次第、
回収口８から取出してもよいし、一定濃度に濃縮された
段階で回収口８から取出してもよい。
実施例２
第３図は、本発明の方法を実施するのに用いる別の装
置例の斜視図であり、分離器11とそれをはさむ一対のヘ
ルムホルツコイル12、12′とから構成されている。第４
図は分離器11の水平断面図である。
分離器11は長さ40cm、直径5cmの大体円筒状であって
一方のヘルムホルツコイル12側の部分が方状に突出して
内部に細菌回収室13が形成されている。分離器11の中央
にナイロンメッシュ製円筒14が配され、その回りは液不
透性材料からなる円筒体15で囲まれているが、この円筒
体15の細菌回収室13側の部分は欠けており、泥水が通過
可能である。そして、ナイロンメッシュ製円筒14と細菌
回収室13との間には、ほぼ半円筒状の濾紙16が設けられ
ている。この装置でもナイロンメッシュ製円筒14は必ず
しも必要ではないが、濾紙16の強度を補強する上で望ま
しいものである。
本発明の方法にしたがって、一対のヘルムホルムコイ
ル12、12′間に磁場が加えられる状態で、細菌を含む泥
水が上部からナイロンメッシュ製円筒14内に注入される
と、泥はナイロンメッシュにより一部、そして濾紙によ
って完全に外周への移動が阻止されるが、細菌は磁場を
感じて濾紙16の方向へ移動し、濾紙16を通過して回収室
13に入る。細菌を分離された泥水は分離器の下部17から
排出される。細菌を含む泥水は分離器11に連続的に注入
し、通過させることによって、回収室13内に分離された
細菌は次第に濃縮されることになる。回収室13に分離さ
れた細菌は分離され次第回収口（図示略）から取出して
もよいし、一定濃度に達した段階で取出してもよい。
この第３図及び第４図に示す装置を用いて、走磁性細
菌を105cells/cm2（泥）含む泥水を種々の流量（ml/mi
n）で処理したところ、第５図に示す結果が得られた。
流量4mm/min以下では約40％以上の分離効率が達成され
た。
〔発明の効果〕
本発明の装置及び方法によると、水底沈殿物中の走磁
性細菌を簡単な装置を用い効率よく分離することができ
る。
本発明の装置及び方法によると、走磁性細菌を分離後
の水底沈殿物の回収も容易であり、再び走磁性細菌の培
養に再利用することができる。Description: TECHNICAL FIELD The present invention relates to an apparatus and a method for efficiently separating magnetotactic bacteria living in water bottom sediments. [Prior art and its problems] Microaerobic bacteria, a kind of microaerobic bacteria, inhabit freshwater or seawater, and are known as bacteria having the property of sensing geomagnetism and swimming along magnetic lines of force (Science, 190 , 337-379 (19
75)). The magnetotactic bacterium contains magnetic particles made of magnetite in a state of being arranged in a chain in the cells. The magnetic particles have a small size of about 500 to 1500 ° and have a single magnetic domain structure. Such magnetic fine particles are expected as magnetic recording materials, drug carriers in the medical field, and the like, but it is almost impossible to synthesize them artificially. Thus, there is a need for an apparatus and method for efficiently cultivating and separating magnetotactic bacteria. As an efficient culture method for magnetotactic bacteria, there is a method proposed by the present inventors in Japanese Patent Application No. 61-273619. Since the culture of magnetotactic bacteria is generally carried out in a submerged sediment, that is, in mud, it is necessary to separate and concentrate the cultured and propagated magnetotactic bacteria from the submerged sediment. Conventionally, as a method or an apparatus for separating magnetotactic bacteria from submerged sediments, for example,
-56074, 61-81778 and 61-81779 are known. [Problems to be Solved by the Invention] However, the above-described conventionally known apparatus and method for separating magnetotactic bacteria have a drawback that the apparatus used is complicated but the separation efficiency is low. Therefore, an object of the present invention is to provide an apparatus and a method capable of efficiently separating magnetotactic bacteria with a simple apparatus. [Means for Solving the Problems] The present invention provides an apparatus for separating magnetotactic bacteria from water containing an aqueous sediment and magnetotactic bacteria, in order to achieve the above object. A container made of a nonmagnetic material, having an inlet for introducing water containing an aqueous sediment and a magnetotactic bacterium, having an outlet at a lower portion, and having a flow passage extending in a substantially vertical direction therein, A porous diaphragm provided in the container in a vertical direction, through which magnetotactic bacteria can pass, but water bottom sediments cannot pass through, and the inside of the container is connected to the flow path by the diaphragm. A compartment for collecting water containing magnetotactic bacteria passing through the diaphragm, and a compartment for collecting bacteria containing magnetotactic bacteria, wherein a compartment for collecting bacteria is provided at a lower portion of the compartment for collecting magnetotactic bacteria. Outside of the container, sandwich the container One said composed with the placed pair of magnets so that the magnetic field is applied in a direction substantially perpendicular with respect to the porous membrane, there is provided a run magnetic bacterium separator. Furthermore, the present invention uses the above-described apparatus, and applies water containing bottom sediment and magnetotactic bacteria to the flow path while applying a magnetic field in a direction substantially perpendicular to the porous diaphragm by the pair of magnets. A method for separating magnetotactic bacteria, comprising the step of flowing and thereby separating the magnetotactic bacteria into the magnetotactic bacteria collection chamber. In the apparatus and method of the present invention, as the porous diaphragm that partitions the flow path, for example, a diaphragm made of a non-metallic material such as filter paper, a glass filter, a ceramic filter, or the like; a diaphragm made of a non-magnetic material such as aluminum is used. The pore size must be large enough to prevent the passage of sediment, but pass through magnetotactic bacteria.
It may be in the range of 1010 μm. To apply a magnetic field in a direction perpendicular to the porous diaphragm in the present invention, for example, an electromagnet such as a Helmholtz coil or a solenoid; or other permanent magnets made of ferrite, metal, or the like can be used. [Examples] Hereinafter, the apparatus and method of the present invention will be specifically described with reference to examples. An example of carrying out the method of the present invention using an apparatus whose perspective view is shown in FIG. 1 will be described. The apparatus shown in FIG. 1 comprises a separator 1 and a pair of Helmholtz coils 2, 2 'arranged at a position sandwiching the separator 1. FIG. 2 is a plan view of the separator 1. The separator 1 is formed of a substantially rectangular parallelepiped container. When water containing magnetotactic bacteria (hereinafter, referred to as “bacteria”) and bottom sediment (hereinafter, referred to as “muddy water”) is injected from above, the bacteria are separated inside. Then, muddy water comes out from the lower outlet 3. Therefore, in the separator 1, a vertical flow path is formed from above to below. The inside of the separator 1 is divided into three chambers by a nylon mesh 4 and a filter paper 5 provided along the direction of the flow path (that is, in the vertical direction). The mud is injected into the left chamber 6 of the nylon mesh 4. The bottom sediment (mud) in the muddy water cannot move to the right side chamber (magnetotactic bacterium recovery chamber) 7 by the nylon mesh 4 and the filter paper 5, but the germs are directed to the direction of the magnetic field formed by the Helmholtz coils 2, 2 ′ ( In the case of north directional bacteria in a direction perpendicular to the filter paper 5, the collection chamber 7 moves to the direction of the south pole, and passes through the filter paper 5 with water and enters the collection chamber 7. The nylon mesh 4 is not necessarily required, but when filter paper is used as the porous membrane, it is desirable to use a nylon mesh or the like in combination since the filter paper alone cannot withstand the load of mud. Thus, when muddy water is continuously injected while applying a magnetic field, bacteria are successively separated into the collection chamber 7 and concentrated. As soon as the bacteria separated in the collection chamber 7 are separated,
It may be taken out from the collecting port 8 or may be taken out from the collecting port 8 at a stage where the concentration is made constant. Embodiment 2 FIG. 3 is a perspective view of another example of an apparatus used to carry out the method of the present invention, which comprises a separator 11 and a pair of Helmholtz coils 12 and 12 'sandwiching the separator. 4th
The figure is a horizontal sectional view of the separator 11. The separator 11 has a substantially cylindrical shape with a length of 40 cm and a diameter of 5 cm, and a portion on the side of one Helmholtz coil 12 protrudes in a square shape, and a bacteria collection chamber 13 is formed inside. A nylon mesh cylinder 14 is disposed at the center of the separator 11, and the periphery thereof is surrounded by a cylinder 15 made of a liquid impermeable material. And muddy water can pass through. A substantially semi-cylindrical filter paper 16 is provided between the nylon mesh cylinder 14 and the bacteria collection chamber 13. Even in this apparatus, the nylon mesh cylinder 14 is not always necessary, but is desirable for reinforcing the strength of the filter paper 16. According to the method of the present invention, when muddy water containing bacteria is injected into the nylon mesh cylinder 14 from above with a magnetic field applied between the pair of Helmholm coils 12, 12 ', the mud is removed by the nylon mesh. And the filter paper completely prevents its movement to the outer periphery, but the bacteria feel the magnetic field and move in the direction of the filter paper 16 and pass through the filter paper 16 to the collection chamber.
Enter 13. The mud from which the bacteria have been separated is discharged from the lower part 17 of the separator. By continuously injecting and passing the mud containing bacteria into the separator 11, the bacteria separated in the collection chamber 13 are gradually concentrated. The bacteria separated in the collection chamber 13 may be taken out from the collection port (not shown) as soon as they are separated, or may be taken out when the concentration reaches a certain concentration. Using the apparatus shown in FIGS. 3 and 4, muddy water containing 10 5 cells / cm 2 (mud) of magnetotactic bacteria at various flow rates (ml / mi
After processing in n), the results shown in FIG. 5 were obtained.
At a flow rate of 4 mm / min or less, a separation efficiency of about 40% or more was achieved. [Effects of the Invention] According to the apparatus and method of the present invention, magnetotactic bacteria in water bottom sediments can be efficiently separated using a simple apparatus. ADVANTAGE OF THE INVENTION According to the apparatus and method of this invention, collection | recovery of the water bottom sediment after isolation | separation of magnetotactic bacteria is also easy, and can be reused for culture | cultivation of magnetotactic bacteria again.
【図面の簡単な説明】
第１図は本発明の装置例の斜視図で第図は同装置の分離
器の平面図である。
第３図は、別の実施例の斜視図で、第４図は同装置の水
平断面図である。
第５図は、第３図の装置を用いて走磁性細菌を105cells
/cm2（泥）含む泥水を処理した際の流量と細菌分離度と
の関係を示す。
１、11……分離器:2、２′、12、12′……ヘルムホルツ
コイル;5、16……濾紙、７、13……走磁性細菌回収室BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a perspective view of an example of the apparatus of the present invention, and FIG. 1 is a plan view of a separator of the apparatus. FIG. 3 is a perspective view of another embodiment, and FIG. 4 is a horizontal sectional view of the apparatus. Fig. 5 shows 10 5 cells of magnetotactic bacteria using the device of Fig. 3.
Fig. 4 shows the relationship between the flow rate and the bacterial separation when muddy water containing / cm 2 (mud) is treated. 1, 11: Separator: 2, 2 ', 12, 12' ... Helmholtz coil; 5, 16 ... Filter paper, 7, 13 ... Magnetic bacteria recovery chamber
───────────────────────────────────────────────────── フロントページの続き (56)参考文献 特開 昭61−81778（ＪＰ，Ａ) 特開 昭62−61599（ＪＰ，Ａ) ────────────────────────────────────────────────── ─── Continuation of front page (56) References JP-A-61-81778 (JP, A) JP-A-62-61599 (JP, A)
Claims (1)
を分離するための装置であって、 上部に前記の水性沈殿物及び走磁性細菌を含む水を投入
する入口を有し、下部にその出口を有し、その内部にほ
ぼ鉛直方向に延びる流路が形成されている、非磁性材料
からなる容器と、 該容器内に鉛直方向に設けられた、走磁性細菌は通過す
ることができるが水底沈殿物は通過することができない
多孔質隔膜とからなり、 前記容器内は該隔膜により前記の流路と、該隔膜を通過
した走磁性細菌を含む水を収容する走磁性細菌回収室と
に仕切られており、さらに該走磁性細菌回収室の下部に
は細菌回収口が設けられており、 さらに、前記容器の外側に、該容器をはさむようにかつ
前記多孔質隔膜に対してほぼ垂直な方向に磁場が加えら
れるように配置された一対の磁石を備えてなる、走磁性
細菌分離装置。 ２．前記請求項１に記載の装置を用い、前記一対の磁石
により前記多孔質隔膜に対してほぼ垂直な方向に磁場を
加えながら前記の流路に水底沈殿物及び走磁性細菌を含
む水を流し、これにより前記走磁性細菌回収室に走磁性
細菌を分離する工程を含む走磁性細菌の分離方法。(57) [Claims] An apparatus for separating magnetotactic bacteria from water containing an aqueous sediment and a magnetotactic bacterium, comprising an inlet at an upper portion for charging the water containing the aqueous sediment and a magnetotactic bacterium, and an outlet at a lower portion thereof A container made of a non-magnetic material, in which a flow path extending in a substantially vertical direction is formed, and a container provided in the container in a vertical direction, through which magnetotactic bacteria can pass but the water bottom The sediment comprises a porous membrane that cannot pass therethrough, and the inside of the vessel is partitioned by the membrane into the above-mentioned flow path and a magnetotactic bacteria collection chamber containing water containing magnetotactic bacteria passing through the membrane. And a bacterium recovery port is provided at a lower portion of the magnetotactic bacterium recovery chamber. Further, a direction substantially perpendicular to the porous membrane so as to sandwich the container outside the container. Arranged so that a magnetic field can be applied to A device for separating magnetotactic bacteria, comprising a pair of magnets. 2. Using the apparatus according to claim 1, flowing water containing a sediment with a bottom sediment and magnetotactic bacteria in the flow path while applying a magnetic field in a direction substantially perpendicular to the porous diaphragm by the pair of magnets, Thus, a method for separating magnetotactic bacteria, comprising the step of separating magnetotactic bacteria in the magnetotactic bacteria collection chamber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62081912A JP2745047B2 (en) | 1987-04-02 | 1987-04-02 | Apparatus and method for separating magnetotactic bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62081912A JP2745047B2 (en) | 1987-04-02 | 1987-04-02 | Apparatus and method for separating magnetotactic bacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63248384A JPS63248384A (en) | 1988-10-14 |
| JP2745047B2 true JP2745047B2 (en) | 1998-04-28 |
Family
ID=13759656
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62081912A Expired - Fee Related JP2745047B2 (en) | 1987-04-02 | 1987-04-02 | Apparatus and method for separating magnetotactic bacteria |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2745047B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103184151A (en) * | 2011-12-30 | 2013-07-03 | 西交利物浦大学 | Device for high-efficient separation of magnetotactic bacteria in high gradient magnetic field |
| CN107287115A (en) * | 2017-08-11 | 2017-10-24 | 兰州理工大学 | A kind of denitrification magnetotactic bacteria is separately cultured device |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6181778A (en) * | 1984-09-28 | 1986-04-25 | Hitachi Ltd | Separation and recover of aquatic microorganism |
| JPH064023B2 (en) * | 1985-09-13 | 1994-01-19 | ティーディーケイ株式会社 | Method and device for collecting magnetotactic bacteria |
-
1987
- 1987-04-02 JP JP62081912A patent/JP2745047B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPS63248384A (en) | 1988-10-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4904391A (en) | Method and apparatus for removal of cells from bone marrow | |
| TWI588262B (en) | Methods and compositions for separating or enriching cells | |
| US12012591B2 (en) | Apparatus and method for immunomagnetic cell separation | |
| AU686257B2 (en) | Method for mammalian cell separation from a mixture of cell populations | |
| US4666595A (en) | Apparatus for acoustically removing particles from a magnetic separation matrix | |
| US20160271314A1 (en) | Device and method for combined microfluidic-micromagnetic separation of material in continuous flow | |
| US5980479A (en) | Method and system for correcting a biological fluid | |
| US20090152176A1 (en) | Magnetic separation of fine particles from compositions | |
| WO1999019071A1 (en) | High gradient magnetic device and method for cell separation or purification | |
| Hirschbein et al. | Magnetic separations in chemistry and biochemistry | |
| Moffat et al. | Selective separations in environmental and industrial processes using magnetic carrier technology | |
| JP2745047B2 (en) | Apparatus and method for separating magnetotactic bacteria | |
| US20190126288A1 (en) | Magnetic separation system and devices | |
| EP0348551A1 (en) | Device and method for the modification of activity of microorganisms contained in liquids without the addition of chemical reagents | |
| US20210170423A1 (en) | Magnetic separation system and devices | |
| CN106477691A (en) | A kind of magnetic suspension fluid bed for water process | |
| Chen et al. | A comprehensive in vitro investigation of a portable magnetic separator device for human blood detoxification | |
| JPH0479614B2 (en) | ||
| JP3460126B2 (en) | Water quality activation device | |
| US20230201831A1 (en) | Method and flow cell for separating biomolecules from liquid medium | |
| JPS6261599A (en) | Collection of magnetotactic bacteria and collecting tool | |
| JPS6181778A (en) | Separation and recover of aquatic microorganism | |
| JP4656830B2 (en) | Solid-liquid separation method and apparatus | |
| JPS634820A (en) | Filtrating apparatus using laminated magnet | |
| JPS63116797A (en) | Device for treating red water in water storage tank |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| S533 | Written request for registration of change of name |
Free format text: JAPANESE INTERMEDIATE CODE: R313533 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| LAPS | Cancellation because of no payment of annual fees |