CN103184151A - Device for high-efficient separation of magnetotactic bacteria in high gradient magnetic field - Google Patents
Device for high-efficient separation of magnetotactic bacteria in high gradient magnetic field Download PDFInfo
- Publication number
- CN103184151A CN103184151A CN2011104515107A CN201110451510A CN103184151A CN 103184151 A CN103184151 A CN 103184151A CN 2011104515107 A CN2011104515107 A CN 2011104515107A CN 201110451510 A CN201110451510 A CN 201110451510A CN 103184151 A CN103184151 A CN 103184151A
- Authority
- CN
- China
- Prior art keywords
- magnetotactic bacteria
- gradient magnetic
- magnetic
- match
- steel wool
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention discloses a device for high-efficient separation of magnetotactic bacteria in a high gradient magnetic field, which comprises a container for accommodating liquid containing magnetotactic bacteria, and is characterized in that the container is disposed in a solenoid; the solenoid is connected with a low frequency power supply; a steel wool with magnetic conductibility is disposed in the container. The device of the invention has a high magnetic field gradient; the magnetic field force applied to the magnetotactic bacteria is hundred times higher than that applied by a permanent magnet, and thus the magnetotactic bacteria can be captured easily, and are not washed away with solutions. The adsorption efficiency is improved greatly. The high gradient magnetic field can be removed at any time by turning off the electromagnetic field, and thus the magnetotactic bacteria are easily washed off from the steel wool, realizing the purpose of separation or recovery. The device is low in technical cost, high in efficiency, and free of secondary pollution.
Description
Technical field
The invention belongs to microorganism separation method and sewage sludge process field, be specifically related to a kind of High-gradient Magnetic device of high efficiency separation magnetotactic bacteria after the match.
Background technology
But magnetotactic bacteria is the microorganism that a class self is made magnetic corpusculum (Z 250).But this bacterioid perception terrestrial magnetic field or foreign field, thereby identification direction; Simultaneously, can be induced by foreign field.Based on this character, can control, lead and catch magnetotactic bacteria by magnetic field.Magnetotactic bacteria can be separated from solution by adding foreign field, be a technology that can have wide application prospects in microorganism and other fields.In addition, discover that magnetotactic bacteria is as microorganism, cell can or adsorb metallics in conjunction with certain metallic particles, and radioelement; Like this, if magnetotactic bacteria can be adsorbed heavy metal earlier, then with the magnetic field guiding, capture magnetotactic bacteria, can remove heavy metal or radioelement in the solution, perhaps precious metal, radioactive metal are carried out recycling.But, present some adopt the method for permanent magnet can't high efficiencyly induce and catch magnetotactic bacteria, Chinese patent application as has been publicly, publication number CN1693223A, induce and catch magnetotactic bacteria with regard to the method that is to use permanent magnet, capture rate is low, makes a lot of bacterium to be taken away by solution, has a strong impact on the separation efficiency of magnetotactic bacteria.
Summary of the invention
The present invention seeks to: a kind of high efficiency magnetotactic bacteria tripping device is provided, even realizing magnetotactic bacteria can both realize high efficiencyly catching and separating under higher solution flow rate, thereby heavy metal, radioactive metal are handled, and fields such as precious metal recovery provide effective means.
Technical scheme of the present invention is: adopt low frequency electromagnetic field in conjunction with the magnetic diffusivity steel wool of vertical direction, realize high-gradient magnetic field, thereby thereby to magnetotactic bacteria realize high-intensity magnetic field power attraction, catch it separated from solution.Electromagnetic field realizes by a solenoid coil that twines container, and magnetic line of force is along the long axis direction of cylindrical chamber, and solid matter steel wool on perpendicular to long axis direction is realized high magnetic field gradients.
Further, cylindrical vessel has fluid inlet and liquid outlet.The solution that contains magnetotactic bacteria will be entered by fluid inlet, and behind the high-gradient magnetic field in the container, the overwhelming majority will be caught and be adsorbed on the steel wool by the high-gradient magnetic field around the steel wool.
Further, cylindrical vessel is formed a solenoid coil by the copper cash compact winding, and magnetic line of force is along the cylindrical chamber long axis direction.
Further, solenoidal copper cash connects low-frequency power, comes controlling magnetic field intensity by regulating size of current, and design magnetic field is 0.01 to 0.1 tesla.
Further, the magnetic diffusivity steel wool is closely placed in container, filling ratio 5% to 10%.Vertical with the cylindrical chamber long axis direction, the relative magnetic permeability of magnetic diffusivity steel wool is 2500 to 5000.
Further, the gradient that forms of the high-gradient magnetic field of being made up of solenoidal inductor and steel wool is every meter of 10 to 1500 tesla (T/m).
During working order, when the magnetotactic bacteria adsorption step finishes, electric current is disconnected, magnetic field disappears, and can the magnetotactic bacteria that adsorb on the steel wool easily be washed away by current, realizes the effect of separating; Steel wool can continue to utilize.
Advantage of the present invention is:
The field gradient height, the suffered magnetic field force of magnetotactic bacteria can be more than hundred times of permanent magnet, can be hunted down easily, and can not washed away by solution.Adsorption efficiency improves greatly.And by closing electromagnetic field, can remove high-gradient magnetic field at any time, make magnetotactic bacteria be easy to be cleaned disengaging from steel wool, realize the purpose of separating or reclaiming.This installs and technical costs is low, efficient is high, non-secondary pollution.
Description of drawings
Below in conjunction with drawings and Examples the present invention is further described:
Fig. 1 is the High-gradient Magnetic of the present invention structure drawing of device of high efficiency separation magnetotactic bacteria after the match.
Fig. 2 is High-gradient Magnetic of the present invention device section and the interior view of high efficiency separation magnetotactic bacteria after the match.
Wherein: 1-1 is cylindrical vessel, 1-2 is that copper cash twines shell of tank formation solenoid coil, 1-3 is the external low-frequency current of copper cash source, 1-4 is fluid inlet, and 1-5 is liquid outlet, and 2-1 is cylindrical vessel, 2-2 is for twining the copper cash section of container, 2-3 is the magnetic diffusivity steel wool, and 2-4 is fluid inlet, and 2-5 is liquid outlet.
Embodiment
Examples of implementation 1:
Device concrete steps and the parameter of making:
Adopt a cylindrical vessel 1-1 as the space of magnetotactic bacteria in the high-gradient magnetic field separation solution.Adopt copper cash compact winding cylindrical vessel to form solenoid coil 1-2.Place magnetic diffusivity steel wool 2-3 in the container, direction is vertical with the body length direction of principal axis.Cylindrical chamber adopts synthetic glass or glass.Cylinder length 400mm-2000mm.Internal diameter is 100mm to 500mm.Keeping length-to-diameter ratio is 4 to 1.Two ends are established fluid inlet (the container left end is 1/4 diameter place apart from the lower edge), liquid outlet (the container right-hand member is 1/4 diameter place apart from the upper edge) respectively.Fluid inlet, liquid outlet internal diameter are 20mm.The specification of copper cash determines according to the size of low-frequency power outward current.
The number of turn that copper cash twines is estimated by rough and ready formula: N=B/ (μ I).Wherein, N is the number of turn on the unit length of required copper cash.The magnetic field size (0.01 to 0.1 tesla) of B for needing.μ is the permeability of vacuum constant.I is the size of current of low-frequency power.For example, under 5 amperes in the electric current, obtain the magnetic field of 0.01 tesla, the number of turn density that needs is about 2000 circle/rice.
Magnetic diffusivity steel wool fiber: 20 microns of diameters; Relative magnetic permeability 2500 to 5000; Coercive force is little, can wash easily after removing electromagnetic field; The vertical long axis direction is placed in container, filling ratio 10%.
Near the magnetic diffusivity steel wool, can produce the high magnetic field gradients of 10 to 1500 teslas every meter (T/m).
Embodiment 2:
Adopt contrive equipment high efficiency separation magnetotactic bacteria from solution.
With pending magnetotactic bacteria (magnetic spirillum AMB-1 (Magnetospirillum sp.StrainAMB-I, available from ATCC, bacterial classification number: ATCC700264)) 10 liters of bacterium liquid slowly inject the made container (length 500mm, diameter 100mm, the copper cash number of turn 1000 circles) of embodiment 1 from fluid inlet.Power-on electric current (5 amperes), axis magnetic field is about 0.01 tesla in the container thereby make.Under near the catching of the High-gradient Magnetic field force the steel wool, most of magnetotactic bacteria will be adhered on the very big steel wool of specific surface area.
Solution is analyzed after the liquid outlet collection and treatment, finds that magnetotactic bacteria all caught by device more than 98%, thereby has realized the purpose of high efficiency separation magnetotactic bacteria in the solution.
Behind powered-down, because magnetic field disappears, the steel wool in the container can be washed, magnetotactic bacteria will be easy to desorption.Steel wool after the cleaning can continue to use.
Embodiment 3:
Adopt contrive equipment separating beavy metal from solution.
With magnetotactic bacteria (magnetic spirillum AMB-1 (Magnetospirillum sp.Strain AMB-I, available from ATCC, bacterial classification number: ATCC700264)) in 10 liters of the bacterium liquid, add 100 milligrams of Ni2+, Cu2+, Pb2+, Cr2+ respectively, fully mix, magnetotactic bacteria will become the magnetotactic bacteria solution of band heavy metal ion in conjunction with heavy metal ion.
To handle in the mode injection device of this solution according to embodiment 2.The magnetotactic bacteria of most of absorption heavy metal will be caught by steel wool.
Solution after the liquid outlet collection and treatment detects concentration of heavy metal ion with atomic absorption spectrum, finds for different ions, can both reach the removal efficient more than 92%.Wherein, Ni2+ is 94%, Cu2+ is 92.5%, Pb2+ is 92%, Cr2+ is 93.5%.
Behind powered-down, because magnetic field disappears, the steel wool in the container can be washed, magnetotactic bacteria will be easy to desorption.Heavy metal ion can the enrichment by collecting magnetotactic bacteria.Steel wool after the cleaning can continue to use.
Above embodiment only is the present invention's a kind of embodiment wherein, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to claim of the present invention.Should be pointed out that for the person of ordinary skill of the art without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (8)
1. the High-gradient Magnetic device of high efficiency separation magnetotactic bacteria after the match comprises the container that contains magnetotactic bacteria liquid for placement, it is characterized in that described container places solenoid coil, and solenoid coil links to each other with low-frequency power, is provided with the magnetic diffusivity steel wool in the described container.
2. the High-gradient Magnetic according to claim 1 device of high efficiency separation magnetotactic bacteria after the match, it is characterized in that described container is cylinder shape, have fluid inlet and liquid outlet, on the described solenoidal copper cash compact winding cylindrical container, magnetic line of force is along the cylindrical chamber long axis direction.
3. the High-gradient Magnetic according to claim 2 device of high efficiency separation magnetotactic bacteria after the match is characterized in that the direction of described magnetic diffusivity steel wool is vertical with solenoidal magnetic line of force direction.
4. the High-gradient Magnetic according to claim 3 device of high efficiency separation magnetotactic bacteria after the match is characterized in that the filling ratio of described magnetic diffusivity steel wool is 5% to 10%.
5. the High-gradient Magnetic according to claim 3 device of high efficiency separation magnetotactic bacteria after the match is characterized in that described solenoidal inductor is 0.01 to 0.1 tesla.
6. the High-gradient Magnetic according to claim 3 device of high efficiency separation magnetotactic bacteria after the match is characterized in that, the field gradient that described solenoid coil and magnetic diffusivity steel wool form is every meter of 10 to 1500 tesla (T/m).
7. the High-gradient Magnetic according to claim 2 device of high efficiency separation magnetotactic bacteria after the match is characterized in that, described cylindrical container adopts synthetic glass or glass to make.
8. the High-gradient Magnetic according to claim 3 device of high efficiency separation magnetotactic bacteria after the match is characterized in that the relative magnetic permeability of described magnetic diffusivity steel wool is 2500 to 5000.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104515107A CN103184151A (en) | 2011-12-30 | 2011-12-30 | Device for high-efficient separation of magnetotactic bacteria in high gradient magnetic field |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104515107A CN103184151A (en) | 2011-12-30 | 2011-12-30 | Device for high-efficient separation of magnetotactic bacteria in high gradient magnetic field |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103184151A true CN103184151A (en) | 2013-07-03 |
Family
ID=48675602
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104515107A Pending CN103184151A (en) | 2011-12-30 | 2011-12-30 | Device for high-efficient separation of magnetotactic bacteria in high gradient magnetic field |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103184151A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105170316A (en) * | 2015-10-09 | 2015-12-23 | 北京科技大学 | Device and method for achieving molecule/ion-level separation of homogeneous mixing liquid through magnetizing force |
| CN108850787A (en) * | 2018-08-31 | 2018-11-23 | 北京中电聚能新技术有限公司 | A kind of solution sterilization algae removal device |
| CN109283326A (en) * | 2018-12-21 | 2019-01-29 | 湖南华腾制药有限公司 | Coupling has the magnetic corpusculum and bio-separation, immunologic detection method of Streptavidin |
| CN108850787B (en) * | 2018-08-31 | 2024-04-30 | 北京中电聚能新技术有限公司 | Solution sterilization algae removal device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63248384A (en) * | 1987-04-02 | 1988-10-14 | Tdk Corp | Separation of magnetotactic bacteria |
| CN101254968A (en) * | 2008-04-10 | 2008-09-03 | 内蒙古科技大学 | Low-frequency high-gradient magnetic field scale inhibiting cleaner |
| US20130203145A1 (en) * | 2010-03-08 | 2013-08-08 | Christopher R. Lambert | Continuous Flow Bioreactor for Magnetically Stabilized Three-Dimensional Tissue Culture |
-
2011
- 2011-12-30 CN CN2011104515107A patent/CN103184151A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63248384A (en) * | 1987-04-02 | 1988-10-14 | Tdk Corp | Separation of magnetotactic bacteria |
| CN101254968A (en) * | 2008-04-10 | 2008-09-03 | 内蒙古科技大学 | Low-frequency high-gradient magnetic field scale inhibiting cleaner |
| US20130203145A1 (en) * | 2010-03-08 | 2013-08-08 | Christopher R. Lambert | Continuous Flow Bioreactor for Magnetically Stabilized Three-Dimensional Tissue Culture |
Non-Patent Citations (1)
| Title |
|---|
| 郭宁: "磁性细菌分离仪的开发与应用", 《中国优秀硕士学位论文全文数据库基础科学辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105170316A (en) * | 2015-10-09 | 2015-12-23 | 北京科技大学 | Device and method for achieving molecule/ion-level separation of homogeneous mixing liquid through magnetizing force |
| CN108850787A (en) * | 2018-08-31 | 2018-11-23 | 北京中电聚能新技术有限公司 | A kind of solution sterilization algae removal device |
| CN108850787B (en) * | 2018-08-31 | 2024-04-30 | 北京中电聚能新技术有限公司 | Solution sterilization algae removal device |
| CN109283326A (en) * | 2018-12-21 | 2019-01-29 | 湖南华腾制药有限公司 | Coupling has the magnetic corpusculum and bio-separation, immunologic detection method of Streptavidin |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Muliwa et al. | Magnetic adsorption separation (MAS) process: An alternative method of extracting Cr (VI) from aqueous solution using polypyrrole coated Fe3O4 nanocomposites | |
| Merino-Martos et al. | Setting up high gradient magnetic separation for combating eutrophication of inland waters | |
| CN102327810B (en) | Pollution-free tube-bundle separation-type permanent-magnet separating device being suitable for separating superfine magnetic particles | |
| CN105439272B (en) | Ferrite MFe2O4Magnetic nanoparticle is for method of removal waste water containing tellurium and application thereof | |
| CN107376838A (en) | A kind of preparation method and application of magnetic active carbon fiber | |
| CN103184151A (en) | Device for high-efficient separation of magnetotactic bacteria in high gradient magnetic field | |
| CN101671631B (en) | Magnetic bacteria separometer | |
| CN103041777A (en) | Fe3O4/CS magnetic material adsorbent, preparation method of Fe3O4/CS magnetic material adsorbent, and sewage treatment method | |
| CN210815695U (en) | High gradient magnetic separator and magnetic separation system | |
| Kheshti et al. | Study and optimization of a high-gradient magnetic separator using flat and lattice plates | |
| CN205761715U (en) | A kind of camellia oleosa seed decorticator with magnetic separation structure | |
| CN204588859U (en) | Circular form sewage filter device | |
| KR102062141B1 (en) | Magnetic Separation Apparatus and Method Using Magnetic Graphene Oxide | |
| CN201815376U (en) | Vertical ring-type pulsating high-gradient superconducting magnetic separator | |
| CN205313310U (en) | Contain graphite industrial wastewater treatment equipment | |
| CN113019689B (en) | Cutting fluid filtering device capable of recycling scrap iron and method thereof | |
| CN204769102U (en) | Magnesite is permanent magnetism high gradient magnetic separator for deironing | |
| CN203540195U (en) | Permanent magnet high gradient filter | |
| CN110180677B (en) | Energy-saving transformation method for high-gradient magnetic separator for mineral separation | |
| CN204324964U (en) | A kind of filter plant based on electromagnetic water treatment | |
| CN102923899A (en) | Method for separating scrap irons and filtrating water through electromagnetic spiral pipe | |
| CN110116048B (en) | Low-power-consumption energy-saving high-gradient magnetic separator for mineral separation | |
| CN209322649U (en) | A kind of sewage flocculation treatment device | |
| KR200239768Y1 (en) | Removal apparatus of magnetic a corpuscle using magnet | |
| KR200469288Y1 (en) | Magnetization filter device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130703 |